CN112142199A - Device and method for improving integrated partial denitrification-anaerobic ammonia oxidation coupling denitrification performance - Google Patents

Abstract

A device and a method for improving the integrated partial denitrification-anaerobic ammonia oxidation coupling denitrification performance belong to the field of sewage treatment. The bottom of the reactor is part of denitrification granular sludge, the anaerobic ammonia oxidation granular sludge is arranged in the middle of the reactor, and the anaerobic ammonia oxidation granular sludge inside the reactor is uniformly mixed with the embedded particles of the acinetobacter JQ 1004. The method comprises the steps of performing intensive starvation culture on acinetobacter JQ1004 to enable the acinetobacter JQ1004 to grow in a stable period and to secrete more Acyl Homoserine Lactone (AHLs) signal molecules, then placing cultured acinetobacter JQ1004 embedded particles and anaerobic ammonia oxidation particle sludge in the same reaction device, wherein the acinetobacter JQ1004 can consume an organic carbon source to reduce nitrate nitrogen under an anoxic condition, the influence of organic matters on anaerobic ammonia oxidation is reduced, and the acyl homoserine lactone signal molecules generated by the acinetobacter JQ1004 can improve the activity of anaerobic ammonia oxidation bacteria.

Description

A device for improving the performance of integrated partial denitrification-anammox coupled denitrification setup and method

technical field

The invention belongs to the field of sewage treatment, in particular to a sewage treatment device and an operation strategy of a sewage treatment device mainly comprising partially denitrifying sludge particles, embedding Acinetobacter JQ1004 (No. .

Background technique

Anammox is a process in which nitrite is used as an electron acceptor to oxidize ammonia to nitrogen. Biological denitrification through anammox can reduce oxygen demand. Part of ammonia can be oxidized without consuming oxygen under anoxic conditions. Therefore, it is widely regarded as a cost-effective wastewater denitrification process. To obtain nitrite as a substrate, anammox is usually combined with partial denitrification (PN), which can theoretically reduce aeration by 60%, organic carbon by 100%, and reduce sludge production by 90% . The partial denitrification/anammox (PDA) process is considered as a promising method to realize anammox in wastewater treatment plants. It should be noted that 11% of nitrogen is converted to nitrate through anammox reaction, and the presence of nitrite oxidizing bacteria (NOB) inevitably exacerbates the accumulation of nitrate in wastewater. However, the PN/anammox process was unable to remove nitrates contained in wastewater, resulting in relatively poor denitrification efficiency (51-87%). In addition, Anammox bacterial cells also have low yield (0.11gVSS/gNH4+-N), long generation period (up to 32d), strong substrate dependence, weak environmental stress resistance and difficulty in gene amplification, which lead to time-consuming reaction initiation. Features that are too long.

Acinetobacter JQ1004 is a Gram-negative strain isolated from the activated sludge sample of the BBR (Bacillus Bacteria Bioreactor) pilot plant of Beijing Gaobeidian Sewage Treatment Plant, which can secrete AHL signaling molecules. It is a kind of facultative bacteria, which can use organic carbon source for denitrification and reduce nitrate nitrogen under anoxic conditions. The suitable living conditions are: pH is about 7.33, temperature is about 31.8 ℃. It has relatively strong resistance to environmental stress, strong proliferation ability, and has great application prospects in the field of sewage treatment.

Compared with the partial denitrification-anammox process, embedding the stable Acinetobacter JQ1004 in the anammox area has the following advantages:

(1) Acinetobacter JQ1004 can reduce nitrate nitrogen under anoxic conditions, which can further improve the effluent quality of partial denitrification-anammox.

(2) The secretion of Acinetobacter JQ1004 contains acyl homoserine lactones (AHLs) signaling molecules, AHLs can promote the expression of anammox activity genes and facilitate the formation of biofilms.

(3) Acinetobacter JQ1004 has strong adaptability to the environment, and JQ1004 in the stable period of inoculation can also have a longer survival time, which can make the system run more stable in the sewage biochemical system.

SUMMARY OF THE INVENTION

Due to partial denitrification, anammox microorganisms are sensitive to environmental changes, such as weak resistance to organic interference or toxic and harmful substances in the matrix, especially when partial denitrification coupled with anammox process is applied to mainstream wastewater treatment, microorganisms The activity was inhibited by organic matter in mainstream wastewater, resulting in incomplete denitrification.

Therefore, the patent of the present invention provides a device and operation method for inoculating embedded particles of Acinetobacter JQ1004 in an integrated partial denitrification coupled anammox area for enhanced denitrification. The main innovation is to intensify the starvation culture of Acinetobacter JQ1004, so that it grows in a stable phase and can secrete more acyl homoserine lactones (AHLs) type signal molecules, and then the cultured stable phase is kept unchanged. Bacillus JQ1004 embedded particles and anammox granular sludge are placed in the same reaction device, and the two are evenly mixed. Acinetobacter JQ1004 can consume organic carbon sources to reduce nitrate nitrogen under anoxic conditions, reducing the effect of organic matter on anaerobic ammonia. At the same time, the acyl homoserine lactones (AHLs) signal molecules produced by Acinetobacter JQ1004 can also enhance the activity of anammox bacteria. The bottom of the reactor is partially denitrifying granular sludge, and the anammox granular sludge is in the middle of the reactor, where the anammox granular sludge and Acinetobacter JQ1004 embedded particles are evenly mixed.

A device for improving the denitrification performance of an integrated partial denitrification-anammox coupling process is characterized in that, comprising:

The reactor is a UASB reactor made of plexiglass. The bottom of the reactor is a part of the denitrification area, in which part of the denitrification granular sludge is inoculated, and polyethylene balls are used as fillers, and the filling ratio is 35%; The upper part is the anammox area, and part of the denitrification area and the anammox area are separated by a partition that allows the solution to pass through; the anammox area is inoculated with anammox granular sludge, and Pall rings and sponges are used. As a filler, the filling ratio is 30%, and JQ1004 embedded particles are also added in the anammox area, and the filling ratio is 15%; the top of the reactor is the three-phase separation area, and the anammox area is separated from the three-phase. The areas are separated by partitions that allow the solution to pass through, and 18 mesh screens are laid on the partitions; the three-phase separation area is equipped with a three-phase separation device and an overflow tank, and the lower part of the three-phase separation area passes through the return port. The return pump is connected to the water inlet at the bottom of part of the denitrification area, and the overflow tank is provided with a water outlet to discharge the overflowed water from the overflow tank through the water outlet;

The upper side of the anammox area is connected and communicated with the constant temperature water bath through the circulation outlet, and the constant temperature water bath is connected and communicated with the circulation inlet of the lower side of the partial denitrification area through the water circulation pump;

The water tank is connected to the water inlet at the bottom of the partial denitrification zone via the water inlet peristaltic pump.

There are pH on-line electrodes and DO on-line electrodes in the anammox zone.

The upper side and the lower side of the anammox area are provided with sampling ports, and some of the side surfaces of the denitrification area are also provided with sampling ports.

The volume ratio of the partial denitrification zone to the anammox zone was 1:2.5.

The water is fed in a continuous flow mode, and the artificial simulated wastewater containing nitrate is pumped from the bottom of the reactor through a peristaltic pump, and partial denitrification is performed to reduce the nitrate to nitrous; Nitrogen and part of nitrate, while JQ1004 bacteria can carry out denitrification to reduce the nitrate produced by the reaction of influent and anammox, and at the same time, the AHLs signal molecules secreted by itself can strengthen the anammox reaction; finally, in the three-phase separation zone , the generated gas is discharged through the exhaust pipe, a part of the effluent is returned to the water inlet of the partial denitrification area at the bottom of the reactor through the reflux port in the three-phase separation zone, and the overflowed water in the overflow tank is discharged through the water outlet.

The preparation and culture of Acinetobacter JQ1004 embedded particles:

(1) Culture and strengthening of Acinetobacter JQ1004:

1) The pure strain JQ1004 stored in 40% glycerol at -80°C was transferred to LB medium for enrichment, and activated at 30°C at 120 r/min for 12 h for later use;

2) Then transfer it to a denitrification medium with a C/N ratio of 7 (100 mL medium in a 250 mL Erlenmeyer flask) at a 5% inoculum volume, and cultivate at 180 r/min at 30 °C for 24 h separation and purification;

3) The strains were then starved for cultivation, and the isolated and purified bacteria were transferred to the denitrification medium with a C/N ratio of 4 at 20% of the inoculum for 6 hours, and then transferred to 20% of the inoculum. C/N ratio of 2.5 in denitrification medium for 6h (other culture conditions and medium components are the same).

4) Finally, take the expanded culture solution of the strain and centrifuge (for example, 100 mL in a high-speed centrifuge at 4 000 r/min for 20 min), discard the supernatant, and repeat washing with normal saline for 2 to 3 times to prepare a suspension of strain JQ1004 for later use;

(2) Production of Acinetobacter JQ1004 embedded particles:

1) Mixing polyvinyl alcohol (PVA), sodium alginate, and deionized water with volume fractions of 10%, 1%, and 89% respectively, and heating to dissolve them completely to obtain a PVA-sodium alginate mixed solution;

2) after the PVA-sodium alginate mixed solution is cooled to room temperature, then the cultured bacterial classification is added to make them evenly mixed; the concentration of the bacterial classification in the PVA-sodium alginate mixed solution is 15-20 mg L ^-1 ;

3) Then add boric acid to it, keep stirring to make the solution reach a saturated state, after the mixed solution gels into a solid state, cut it into small cubes of 4mm×4mm×4mm, and add it to the calcium chloride solution of 3-5wt% , during this period, in order to prevent the small cubes from sticking, it is necessary to continuously stir; then cross-link at 4 °C for 24 hours. After the cross-linking, take out the small cubes and wash them with deionized water for 2-3 times, and then store them in a refrigerator at 4 °C. Refrigerate for later use.

Among them, the culture of anammox bacteria:

Inoculate anammox granular sludge into a 100L UASB reactor, set the reactor temperature at 28-32°C, maintain pH at 7.1-7.3, DO less than 0.4mg·L ^-1 , use simulated wastewater for domestication and culture, anaerobic ammonia The hydraulic retention time (HRT) of the oxidative bacterial culture was 12 h.

The composition of the simulated wastewater is as follows: NH ₄ ⁺ -N (calculated as N) 45-60 mg·L ^-1 , NO ₂ ^- -N (calculated as N) 55-80 mg·L ^{-1 ,} KH ₂ PO ₄ 20 mg·L -1 ^1. MgSO ₄ ·7H ₂ O 90mg·L ^-1 , NaHCO ₃ 460mg·L ^-1 , CaCl ₂ ·2H ₂ O 25mg·L ^-1 , the content of trace element solution I 1ml/L, the content of trace element solution II 1ml/L;

The composition of trace element solution I: EDTA 5g·L ^-1 and FeSO ₄ 5g·L ^-1 ;

The composition of trace element solution II: EDTA 15g·L ^-1 , ZnSO ₄ ·7H ₂ O 0.43g·L ^-1 , MnCl2·4H ₂ O 0.99g·L ^{-1 ,} CuSO ₄ ·5H ₂ O 0.25g·L -1 ¹ , NaMoO ₄ ·2H ₂ O 0.22g·L ^-1 , NiCl ₂ ·6H ₂ O 0.19g·L ^-1 and H ₃ BO ₄ 0.014g·L ^-1 .

Some of the denitrifying bacteria were cultured:

The sludge inoculated with partial denitrification is taken from the anoxic tank of the A/A/O process, such as from the anoxic tank of the A/A/O process of the Beijing Gaobeidian Sewage Treatment Plant, and the recovered sludge is first removed by standing. The supernatant was filtered off with a fine-mesh sieve, and then it was inoculated into the SBR reactor. The MLSS concentration of the inoculated sludge in the reactor was 4740 mg·L ^-1 , and the MLVSS concentration was 3077 mg·L ^-1 . MLVSS/MLSS is 0.65, and then it starts to run under artificial water distribution, the temperature is controlled at about 28 °C, the pH is maintained at about 7.5, the drainage ratio is 50%, and the DO is less than 0.5 mg·L ^-1 . The mud settling performance is good, the appearance is gray and flocculent, and the accumulation rate of nitrous is stable above 70%.

Among them, artificial water distribution: NO ₃ ^- -N (calculated by N) content is 65-80 mg·L ^-1 , anhydrous sodium acetate (calculated by COD) 200-250 mg·L ^-1 (COD/NO ₃ ^- -N is 3 ), NH ₄ ⁺ -N (calculated as N) 20-30mg·L ^-1 , KH ₂ PO ₄ 20mg·L ^-1 , MgSO ₄ ·7H ₂ O 90mg·L ^-1 , CaCl ₂ ·2H ₂ O 25mg·L ^-1 . The content of trace element solution I is 1ml/L, the content of trace element solution II is 1ml/L,

The composition of trace element solution I: EDTA 5g·L ^-1 and FeSO ₄ 5g·L ^-1 ;

The composition of trace element solution II: EDTA 15g·L ^-1 , ZnSO ₄ ·7H ₂ O 0.43g·L ^-1 , MnCl ₂ ·4H ₂ O 0.99g·L ^-1 , CuSO ₄ ·5H ₂ O 0.25g·L -1 ⁻¹ , NaMoO ₄ ·2H ₂ O 0.22 g·L ⁻¹ , NiCl ₂ ·6H ₂ O 0.19 g·L ⁻¹ , and H ₃ BO ₄ 0.014 g·L ⁻¹ .

Operation of the reactor

The reactor is a UASB reactor made of plexiglass, and the water is fed in a continuous flow; the bottom of the reactor is a partial denitrification area, into which the partial denitrification granular sludge cultivated in the inoculation step is inoculated, and the concentration of the inoculated sludge is 3000. -4000mg·L ^-1 , with polyethylene as filler, the filling ratio is 25%-35%; followed by the anammox area containing the embedded particles of Acinetobacter JQ1004, inoculated with the cultured anammox granular sludge , the concentration of the inoculated sludge is 2000-4000 mg·L ^-1 , and the Pall ring and sponge are wrapped with polypropylene spheroids as fillers, preferably the volume ratio of the two is 1:1, and the filling ratio is 25%-30%, At the same time, the embedded particles of Acinetobacter JQ1004 were evenly added, and the filling ratio was 10%-15%; the top part was a three-phase separation area, and the areas were separated by a partition, and an 18-mesh screen was laid on the partition.

The method for improving partial denitrification-anammox coupled denitrification performance by using the above device is characterized in that, comprising the following steps:

The artificial simulated wastewater containing nitrate is pumped from the bottom of the reactor through a peristaltic pump, and partial denitrification is performed to reduce nitrate to nitrous; JQ1004 bacteria can carry out denitrification to reduce the nitrate produced by the reaction of influent and anammox, and at the same time, the AHLs signal molecules secreted by itself can strengthen the anammox reaction; finally, in the three-phase separation zone, the generated gas passes through the exhaust gas. The pipe is discharged, and a part of the effluent is returned to the water inlet of the partial denitrification area at the bottom of the reactor through the reflux port in the three-phase separation zone, and the overflowed water in the overflow tank is discharged through the water outlet.

(1) The start-up period of the reactor is divided into two stages: in the first stage, the duration is 50-60 days, and the hydraulic retention time (HRT) of the UASB reactor is 20-24h; in the second stage, the duration is 50-80 days, which promotes PDA The biomass in the reactor increased and the reactor obtained a high nitrogen removal rate (NRR), increasing the inflow rate from 6.25-7.5L·h ^-1 to 12.5L·h ^-1 , which reduced the HRT from 20-24h to 12h; The second-stage biofilm was formed, the sludge concentration in the reactor was stable, the removal effect of effluent COD, NH ₄ ⁺ -N, NO ₃ ^- -N was stable, and the reactor was successfully started.

(2) Reactor operating condition control:

According to the direction of water flow, it is a partial denitrification zone, an anaerobic ammonium oxidation zone containing particles embedded in Acinetobacter JQ1004, and a three-phase separation zone; the hydraulic retention time is 12h.

The temperature of the reactor was controlled at 28-32°C, the pH was at 7.3-7.8, the DO was controlled at 0.1-0.4 mg·L ^-1 , and the sludge reflux ratio was between 30% and 55%.

The partial denitrification/anammox treatment of low carbon nitrogen involved in the present invention has the following advantages over urban sewage:

(1) Simultaneous denitrification, carbon and nitrogen removal can be accomplished in a single-stage reactor;

(2) The pre-partial denitrification removes the organic matter in the raw water and provides a substrate for the anammox bacteria at the same time;

(3) Acinetobacter JQ1004 can perform denitrification under both anoxic and aerobic conditions, and can reduce both nitrite and nitrate under anoxic conditions. This can further improve partial denitrification- Anaerobic ammonia oxidation effluent water quality, to achieve advanced treatment.

(4) The secretion of Acinetobacter JQ1004 contains acyl homoserine lactones (AHLs) type signal molecules, AHLs can promote the expression of anammox genes and facilitate the formation of biofilms.

Description of drawings

1 is a schematic structural diagram of improving an integrated partial denitrification-anammox coupled denitrification process according to an embodiment of the present invention;

Among them: 1—manual water distribution tank; 2—water inlet peristaltic pump; 3—water inlet; 4—polyethylene packing; 5—sampling port; 6—clapboard; 7—reactor constant temperature water bath layer; Sponge adjustment filler; 9—Acinetobacter JQ1004 embedded particles; 10—pH online electrode; 11—DO online electrode; 12—three-phase separation device; 13—water outlet; 14—overflow tank; 15—return port; 16 - return pump; 17 - water bath circulation pump; 18 - constant temperature water bath;

Specific implementation method:

The present invention will be further described below with reference to the examples, but the present invention is not limited to the following examples.

The Acinetobacter JQ1004 was deposited in the General Microbiology Center (CGMCC) of the China Microorganism Culture Collection Management Committee (CGMCC) on March 06, 2018, and the deposit number is CGMCC No. 15414, and the reported patent 201811084608.1 has been published.

Example 1

The specific structure is shown in Figure 1.

(1) Medium composition of Acinetobacter JQ1004

LB medium: NaCl 10.00g·L ^-1 , tryptone 10.00g·L ^-1 , yeast extract 5.00g·L ^-1 ;

Denitrification medium (separation and purification medium): (CH ₂ COONa) ₂ ·6H ₂ O 4.136g·L ^-1 , KNO ₃ 0.72g·L ^-1 , 0.565g·L ^-1 K ₂ HPO ₄ 0.565g· L-1, MgSO ₄ ·7H ₂ O 0.20g·L ^-1 , 2.50mL trace elements, trace element solution: Na ₂ EDTA 50.00g·L ^-1 , ZnSO ₄ ·7H ₂ O 2.20g·L ^-1 ,CaCl ₂ 5.50g·L ^-1 , MnCl·4H ₂ O 5.06g·L ^-1 , FeSO4 5.00g·L ^-1 , CuSO ₄ ·5H ₂ O 1.57g·L ^-1 , CoCl ₂ ·6H ₂ O 1.60g· L ^-1 , both media should be sterilized at 121°C for 15min before use, and the pH should be adjusted between 7.0 and 7.3 by Na ₂ HPO ₄ and NaH ₂ PO ₄ buffer solutions. The solid medium needs to be added with 1.5%～ 2% agar.

(2) Culture of anammox bacteria

Using artificial simulated domestic sewage to cultivate bacteria, the water distribution components are NH ₄ ⁺ -N (calculated as N) 45-60 mg·L ^-1 , NO ₂ ^- -N (calculated as N) 55-70 mg·L ^-1 , KH ₂ PO ₄ 20mg·L ^-1 , MgSO ₄ ·7H ₂ O 90mg·L ^-1 , NaHCO ₃ 460mg·L ^-1 , CaCl ₂ ·2H ₂ O 25mg·L ^-1 ; Trace element solution I: 1ml/L , EDTA 5g·L ^-1 and FeSO ₄ 5g·L ^-1 ;

Trace element solution II: 1ml/L, EDTA 15g·L ^-1 , ZnSO ₄ ·7H ₂ O 0.43g·L ^-1 , MnCl ₂ ·4H2O 0.99g·L ^-1 , CuSO ₄ ·5H ₂ O 0.25g·L -1 ^-1 , NaMoO ₄ ·2H ₂ O 0.22g·L ^-1 , NiCl ₂ ·6H ₂ O 0.19g·L ^-1 and H ₃ BO ₄ 0.014g·L ^-1 , the hydraulic retention time (HRT) was 12h, and the temperature was 28-32℃, pH maintained at 7.1-7.3, DO less than 0.4mg·L ^-1 , lower concentration of influent and higher HRT at the initial stage of reactor start-up; after the reactor is successfully started, the concentration of influent can be increased and the HRT can be reduced . After the anaerobic ammonium oxide sludge culture is completed, the denitrification rate of the UASB reactor under stable operation is over 90%, and the sludge particle size is 2-3mm.

(3) Operation of UASB reactor

1) Reactor startup:

The reactor start-up period is divided into two phases. In the first stage (duration 50-60 days), the UASB reactor was operated at an inflow rate of 6.25–7.5 L·h ⁻¹ , resulting in a hydraulic retention time (HRT) of 20–24 h. In the second stage (duration 50-80 days), to promote the biomass growth in the PDA and obtain a high nitrogen removal rate (NRR) in the reactor, the inflow rate increased from 6.25-7.5 L·h ^-1 to 12.5 L·h ^-1 , resulting in a reduction in HRT from 20-24h to 12h. The biofilm was formed in the second stage, the sludge concentration in the reactor was stable, the removal effect of effluent COD, NH ₄ ⁺ -N and NO ₃ ^- -N was stable, and the reactor was successfully started.

2) Reactor operating condition control:

The reactor is as shown in the figure above. According to the direction of water flow, it is divided into partial denitrification zone, Acinetobacter JQ1004 embedded particle-anammox zone, and three-phase separation zone; the effective volume is 150L, the hydraulic retention time is 12h, and the partial denitrification zone and non- The volume ratio of the embedded particle-anammox zone of the accinetobacter JQ1004 was 1:2.5. The temperature of the reactor was controlled at 28-32°C, the pH was at 7.3-7.8, the DO was controlled at 0.1-0.4 mg·L ^-1 , and the sludge reflux ratio was between 30% and 55%.

The components of the simulated domestic sewage in the influent of the reactor are: NH ₄ Cl (calculated as N) 45-60 mg·L ^-1 , anhydrous sodium acetate (calculated as COD) 200-250 mg·L ^-1 , NO ₃ ^- -N (calculated as COD) 200-250 mg·L -1 N) content is 65-80mg·L ^-1 , NaHCO ₃ is 460mg·L ^-1 , CaCl ₂ 25mg·L ^-1 , KH ₂ PO ₄ 20mg·L ^-1 , MgSO ₄ 7H ₂ O 90mg·L -1 ^{- 1} .

The influent water flows in from the lower part of the reactor, ① firstly passes through the partial denitrification area: the filling ratio of polyethylene (Φ25mm×10 mm) packing is 25%-35%, and the nitrate is converted into nitrite through partial denitrification; ②then feed water Embedding particles by Acinetobacter JQ1004-anammox area: sponge filler (the material is hydrophilic polyurethane, 20mm×20mm×20mm cube) and Pall ring filler (25mm×25mm×3mm) according to 1:1 The proportion is wrapped with ionized ball (material is polypropylene, Φ100mm), the filling ratio is about 25%-30%, JQ1004 is embedded with small cubes (4mm×4mm×4mm cube), the filling ratio is 10%-15%, nitrous acid Salt and ammonia nitrogen are converted into nitrogen and a small amount of nitrate, and Acinetobacter JQ1004 can also denitrify the generated nitrate into nitrous for anaerobic ammonium oxidation reaction; The gas and the effluent are separated by a three-phase separation device.

In the first stage of the actual operation, the COD, NH ₄ ⁺ -N and NO ₃ ^- -N contents of the inlet and outlet water were regularly measured every 3 days to check the removal effect of the reactor. By adjusting the mass concentration ratio of initial COD and nitrate within the range of 2.5 to 3.5, adjusting the reflux pump to appropriately increase the reflux ratio to reduce the influent load, so that the NO ₃ ^- content in the effluent is lower than 1 mg·L ^-1 ; The COD, NH ₄ ⁺ -N and NO ₃ ^- -N contents of the effluent were measured regularly every 7 days, and the reflux ratio was reduced to increase the influent load, so that the nitrogen removal rate of the reactor remained stable.

4) Denitrification effect of coupling process:

After 140 days of operation, the reactor can achieve simultaneous denitrification, carbon and nitrogen removal, and the treatment effect is stable. The removal rates of COD, NH ₄ ⁺ -N and NO ₃ ^- -N are as high as 86.3%, 95.7% and 98.9%, respectively.

Claims (9)

1. The utility model provides an improve device of integral type partial denitrification-anaerobic ammonium oxidation coupling process denitrogenation performance which characterized in that includes:

the reactor is a UASB reactor made of organic slope glass, the bottommost part of the reactor is a partial denitrification area, partial denitrification granular sludge is inoculated, and polyethylene balls are used as fillers; an anaerobic ammonia oxidation area is arranged above part of the denitrification area, and the part of the denitrification area is separated from the anaerobic ammonia oxidation area by a partition plate which can allow a solution to pass through; inoculating anaerobic ammonium oxidation granular sludge in an anaerobic ammonium oxidation area, adopting pall rings and sponges as fillers, wherein the filling ratio is 30%, and simultaneously adding JQ1004 embedding particles into the anaerobic ammonium oxidation area; the top of the reactor is a three-phase separation area, the anaerobic ammonia oxidation area and the three-phase separation area are separated by a partition board through which the solution can pass, and a screen mesh with 18 meshes is paved on the partition board; the three-phase separation area is internally provided with a three-phase separation device and is provided with an overflow groove, the lower part of the three-phase separation area is connected with a water inlet at the bottom of part of the denitrification area through a reflux port and a reflux pump, and the overflow groove is provided with a water outlet for discharging water overflowing from the overflow groove;

the side surface of the upper part of the anaerobic ammonia oxidation area is connected and communicated with a constant-temperature water bath through a circulating outlet, and the constant-temperature water bath is connected and communicated with a circulating inlet on the side surface of the lower part of the denitrification area through a water area circulating pump;

the water tank is connected with a water inlet at the bottom of the partial denitrification area through a water inlet peristaltic pump.

2. The device for improving the denitrification performance of the integrated partial denitrification-anammox coupling process according to claim 1, wherein a pH on-line electrode and a DO on-line electrode are arranged in the anammox area.

3. The apparatus for improving the denitrification performance of the integrated partial denitrification-anammox coupling process in claim 1, wherein the upper side and the lower side of the anammox zone are provided with sampling ports, and the side of the partial denitrification zone is also provided with sampling ports.

4. The device for improving the denitrification performance of the integrated partial denitrification-anaerobic ammonia oxidation coupling process according to claim 1, wherein the volume ratio of the partial denitrification area to the anaerobic ammonia oxidation area is 1: 2.5.

5. The apparatus for improving the denitrification performance of the integrated partial denitrification-anammox coupled process according to claim 1, wherein the preparation and culture of the embedded particles of acinetobacter JQ 1004:

(1) culturing and strengthening acinetobacter JQ 1004:

1) transferring pure strain JQ1004 preserved in 40% glycerol at-80 deg.C to LB culture medium for enrichment, and activating at 30 deg.C and 120r/min for 12 h;

2) then transferring the strain to a denitrification culture medium with a C/N ratio of 7 by using an inoculum size of 5 percent, and culturing the strain at 30 ℃ for 24 hours at 180r/min for separation and purification;

3) then, starvation culture is carried out on the bacterial strain, the separated and purified bacteria are transferred to a denitrification culture medium with a C/N ratio of 4 for culture for 6 hours in an inoculation amount of 20%, and then the bacteria are transferred to a denitrification culture medium with a C/N ratio of 2.5 for culture for 6 hours in an inoculation amount of 20%;

4) finally, taking an expanded culture solution of the strain, centrifuging, removing a supernatant, and repeatedly washing with physiological saline for 2-3 times to prepare a JQ1004 suspension of the strain for later use;

(2) preparation of Acinetobacter JQ1004 embedded particles:

1) respectively mixing 10%, 1% and 89% by volume of mixed solution of polyvinyl alcohol (PVA), sodium alginate and deionized water, and heating to completely dissolve the mixed solution to obtain PVA-sodium alginate mixed solution;

2) after the PVA-sodium alginate mixed solution is cooled to room temperature, adding the cultured strains, and uniformly mixing the strains; the concentration of the strain in the PVA-sodium alginate mixed solution is 15-20 mg.L^-1；

3) Then adding boric acid into the solution, continuously stirring the solution to enable the solution to reach a saturated state, cutting the mixed solution into 4mm multiplied by 4mm small cubes after the mixed solution gel is solid, adding the small cubes into 3-5 wt% of calcium chloride solution, and continuously stirring the small cubes during the period of time to prevent the small cubes from being bonded; then crosslinking for 24h at 4 ℃, taking out the small cubes after crosslinking, washing the small cubes with deionized water for 2-3 times, and storing the small cubes in a refrigerator refrigerating chamber at 4 ℃ for later use.

6. The apparatus of claim 1, wherein the culture of anammox bacteria:

inoculating anaerobic ammonium oxidation granular sludge into a 100L UASB reactor, setting the temperature of the reactor at 28-32 ℃, keeping the pH at 7.1-7.3, and keeping the DO less than 0.4 mg.L^-1Carrying out acclimatization culture by using simulated wastewater, wherein the Hydraulic Retention Time (HRT) of the anaerobic ammonium oxidation bacteria culture is 12 h;

the simulated wastewater comprises the following components: NH (NH)₄ ⁺-N (calculated as N) 45-60 mg.L^-1、NO₂ ^–-N (calculated as N) 55-80 mg.L^-1、KH₂PO₄ 20mg·L^-1、MgSO₄·7H₂O 90mg·L^-1、NaHCO₃ 460mg·L^-1、CaCl₂·2H₂O 25mg·L^-1The content of the trace element solution I is 1ml/L, and the content of the trace element solution II is 1 ml/L;

composition of the trace element solution i: EDTA 5 g.L^-1And FeSO₄ 5g·L^-1；

Composition of microelement solution II: EDTA 15 g.L^-1、ZnSO₄·7H₂O 0.43g·L^-1、MnCl2·4H₂O 0.99g·L^-1、CuSO₄·5H₂O 0.25g·L^-1，NaMoO₄·2H₂O 0.22g·L^-1、NiCl₂·6H₂O 0.19g·L^-1And H₃BO₄ 0.014g·L^-1；

Wherein, part of denitrifying bacteria are cultured:

process for taking inoculated partial denitrification sludge from A/A/OAn anoxic tank, such as an anoxic tank of A/A/O process from sewage treatment plant of Beijing high tombstone shop, is prepared by standing the taken-back sludge, removing supernatant, filtering out dross by using a fine-mesh sieve, inoculating the scum into an SBR reactor, and inoculating the sludge in the SBR reactor to obtain MLSS with the concentration of 4740 mg.L^-1The MLVSS concentration is 3077 mg.L^-1MLVSS/MLSS is 0.65, then the operation is started under the condition of manual water distribution, the temperature is controlled to be about 28 ℃, the pH is kept to be about 7.5, the drainage ratio is 50 percent, and DO is less than 0.5 mg.L^-1After long-term operation, the sludge settling performance in the reactor is good, the appearance is in a gray floccule shape, and the accumulation rate of the nitrite is stabilized to be more than 70%;

wherein, manual water distribution: NO₃ ^-The content of-N (calculated as N) is 65-80 mg.L^-1Anhydrous sodium acetate (calculated as COD) 200-^-1(COD/NO₃ ^--N is 3), NH₄ ⁺-N (calculated as N) 20-30 mg.L^-1、KH₂PO₄ 20mg·L^-1、MgSO₄·7H₂O 90mg·L^-1、CaCl₂·2H₂O 25mg·L^-1The content of the trace element solution I is 1ml/L, the content of the trace element solution II is 1ml/L,

composition of the trace element solution i: EDTA 5 g.L^-1And FeSO₄ 5g·L^-1；

Composition of microelement solution II: EDTA 15 g.L^-1、ZnSO₄·7H₂O 0.43g·L^-1、MnCl₂·4H₂O 0.99g·L^-1、CuSO₄·5H₂O 0.25g·L^-1，NaMoO₄·2H₂O 0.22g·L^-1、NiCl₂·6H₂O 0.19g·L^-1And H₃BO₄ 0.014g·L^-1。

7. The apparatus for improving the denitrification performance of the integrated partial denitrification-anaerobic ammonia oxidation coupling process according to claim 1, wherein the reactor is an UASB reactor made of organic glass and is fed with water in a continuous flow manner; the bottom of the reactor is a partial denitrification zone to which the seeding step is appliedThe concentration of the inoculated sludge of the cultured partial denitrification granular sludge is 3000-4000 mg.L^-1Polyethylene is used as a filler, and the filling ratio is 25-35%; then an anaerobic ammonia oxidation area containing Acinetobacter JQ1004 embedded particles is arranged, cultured anaerobic ammonia oxidation granular sludge is inoculated into the anaerobic ammonia oxidation area, and the concentration of the inoculated sludge is 2000-4000 mg.L^-1And (3) wrapping pall rings and sponge by using flow separation balls made of polypropylene as fillers, wherein the volume ratio of the flow separation balls to the sponge is preferably 1: 1, the filling ratio is 25-30%, and meanwhile, acinetobacter JQ1004 embedding particles are uniformly added, wherein the filling ratio is 10-15%; the top is a three-phase separation area, the areas are separated by a partition plate, and a screen mesh with 18 meshes is laid on the partition plate.

8. The method for improving partial denitrification-anaerobic ammonium oxidation coupling denitrification performance by adopting the device of claims 1-7 is characterized by comprising the following steps:

pumping artificial simulation wastewater containing nitrate from the bottom of the reactor through a peristaltic pump, and carrying out partial denitrification to reduce nitrate nitrogen into nitrite nitrogen; then, ammonia nitrogen and nitrite are converted into nitrogen and partial nitrate through anaerobic ammonia oxidation reaction, JQ1004 bacteria can carry out denitrification to reduce the nitrate generated by the reaction of water inflow and anaerobic ammonia oxidation, and AHLs signal molecules secreted by the JQ1004 bacteria can strengthen the anaerobic ammonia oxidation reaction; and finally, in the three-phase separation area, the generated gas is discharged through an exhaust pipe, part of effluent flows back to a water inlet of a partial denitrification area at the bottom of the reactor through a return port in the three-phase separation area, and the overflow water in the overflow groove is discharged through a water outlet.

9. The method of claim 8,

the reactor start-up period is divided into two phases: in the first stage, the time is 50-60 days, and the Hydraulic Retention Time (HRT) of the UASB reactor is 20-24 h; the duration of the second stage is 50-80 days, the biomass growth in PDA is promoted, the reactor obtains high denitrification rate (NRR), and the inflow speed is increased from 6.25-7.5 L.h^-1Increased to 12.5 L.h^-1Reducing the HRT from 20-24h to 12 h; second stage biofilm formation, reactionThe sludge concentration in the device is stable, and the effluent COD and NH₄ ⁺-N、NO₃ ^-The N removal effect is stable, and the reactor is started successfully. Controlling the operating conditions of the reactor:

according to the water flow direction, a partial denitrification area, an anaerobic ammonia oxidation area containing Acinetobacter JQ1004 embedded particles and a three-phase separation area are sequentially arranged; the hydraulic retention time is 12 h;

the reactor temperature is controlled at 28-32 deg.C, pH is 7.3-7.8, and DO is controlled at 0.1-0.4 mg.L^-1The sludge reflux ratio is 30-55%.

Images