CN112105920A - Method for testing whether grease can permeate into hair and permeation capacity - Google Patents

Abstract

The present disclosure relates to a test method comprising step S1, treating a blank hair bundle 1 with a grease a or b for no more than 10 min; s2, rubbing the hair strands with a surfactant solution, washing with water, and drying in the air; s3, taking the organic solvent soaked in the hair bundle 1 to be tested to perform a nuclear magnetic hydrogen spectrum test, and observing whether the hydrogen spectrum peaks at the comparison position; s4, if the hair bundle 1 to be measured does not peak at the comparison position, carrying out the step S5; if so, repeating the step S2 until the nuclear magnetic hydrogen spectrum in the step S3 does not generate a peak at the comparison position; s5, treating the blank hair bundle 2 with the grease a or b for at least 10min, and repeating the steps S2 and S3 by the same method; if the hair bundle 2 to be measured has a peak at the comparison position, it is judged that the grease a or b can penetrate into the hair, otherwise, it is judged that the grease a or b cannot penetrate into the hair. The method is simple and convenient to operate, low in cost and reliable and effective in test result.

Description

A method to test whether oil can penetrate into the hair and how much it penetrates

technical field

The present disclosure relates to a testing method, in particular to a method for testing whether grease can penetrate into the hair and the penetration ability.

Background technique

Oils, especially vegetable oils, have been widely used in hair care products because of their mildness and unique efficacy. According to research reports, vegetable oil can not only adhere to the surface of the hair, but also penetrate into the inside of the hair, playing a dual-effect care effect. Although the molecular weight and structure of vegetable oils that can penetrate into the hair are still inconclusive, but the vegetable oils whose main components are C8-C18 fatty acid triglycerides, such as coconut oil, have been proved theoretically or practically to penetrate into the hair. internal.

However, there are still technical difficulties in characterizing whether vegetable oils have penetrated into the hair, because the vegetable oils attached to the hair surface can cause huge interference in the analysis. A commonly used and effective characterization and analysis method is time-of-flight secondary ion mass spectrometry (TOF-SIMS), which uses a positively charged gallium ion beam to bombard a hair section to obtain a secondary ion mass spectrum. By identifying the characteristic peaks of vegetable oil in the spectrum, it can be determined whether vegetable oil has penetrated into the hair. (J. Cosmet. Sci 52, 169-184, 2001).

Considering the high cost and cumbersome operation of TOF-SIMS, it is still necessary to explore a simple, inexpensive and effective method to test whether vegetable oils can penetrate into hair.

SUMMARY OF THE INVENTION

In order to overcome the defects of the prior art, the present disclosure provides a new method for testing whether oil can penetrate into the hair. Compared with TOF-SIMS, it has the advantages of simple operation and low cost. Methods, such as the method of judging whether the oil can penetrate into the hair by testing the hair diameter change through a microscope, the test results are more reliable and effective; further, the new method can also show the ability of oil to penetrate into the hair, which can be used to screen the ability of oil to penetrate into the hair. Fast grease.

In the present disclosure, the term "blank tress" refers to tresses that have not been treated with oils, especially vegetable oils, which can be any tress as long as it has not been treated with oils, for example, it can be taken from any age group , Men's or women's hair tresses of any country or region, either healthy tresses or damaged tresses such as mechanically damaged tresses, thermally damaged tresses, chemically damaged tresses, environmentally damaged tresses Tresses, aging tresses. The term "treating" includes, but is not limited to, coating, soaking, and the like. Hair tresses that have been cumulatively treated with grease for no more than 10 minutes, especially no more than 5 minutes, and then rinsed with the surfactant solution are also within the scope of blank tresses of the present disclosure.

In the present disclosure, "capable of dissolving fats and oils" means that the solubility of fats and oils in a solvent at 20°C is greater than 1 g, preferably greater than 5 g, especially greater than 10 g. The term "solubility" refers to the mass of a substance that dissolves in a saturated state in 100 g of solvent at a certain temperature.

A test method provided by the present disclosure includes the following steps:

S1. Treat the blank tress 1 with grease a or b for no more than 10 minutes;

S2, rub the treated hair tress with a surfactant solution, rinse with water, air dry, and wait for testing;

S3, get the organic solvent immersed in the hair beam 1 to be tested and carry out the hydrogen NMR spectrum test, observe whether the obtained hydrogen NMR spectrum peaks at the comparison position, and the comparison position is located at the 1H NMR spectrum of the test solvent immersed in the blank hair beam 1. The peak area is located in the peak area of the hydrogen NMR spectrum of the grease a or b; the organic solvent for soaking the hair beam 1 to be measured and the test solvent for the hydrogen NMR spectrum are solvents that can dissolve the grease;

S4. If the hair beam 1 to be measured does not show a peak at the comparison position, then go to step S5; if there is a peak, repeat step S2 until the H NMR spectrum measured in step S3 shows no peak at the comparison position, then go to step S5 ;

S5. Treat the blank tress 2 with grease a or b for at least 10 min, and rub the tress 2 with the same method of step S2. 2; if the hair bundle 2 to be tested has a peak at the comparison position, it is determined that the oil a or b can penetrate into the hair, otherwise it is determined that the oil a or b cannot penetrate into the hair.

In the present disclosure, the oils and fats include natural oils and synthetic oils, preferably natural oils. The natural oils include animal oils and vegetable oils, preferably vegetable oils. The term "vegetable oil" refers to oils and fats extracted from the roots, stems, leaves, flowers, fruits or seeds of plants, including but not limited to palm oil, palm kernel oil, coconut oil, olive oil, jojoba oil, argan oil, black seed Grass Oil, Bearberry Oil, Crabapple Oil, Shea Butter, Almond Oil, Apricot Walnut Oil, Avocado Oil, Walnut Oil, Castor Oil, Corn Oil, Oat Oil, Cottonseed Oil, Canola Oil, Linseed Oil , Grapeseed Oil, Pomegranate Seed Oil, Citrus Seed Oil, Wheat Germ Oil, Cashew Oil, Pine Nut Oil, Macadamia Oil, Peanut Oil, Soybean Oil, Sesame Oil, Sunflower Oil, Safflower Oil, Tea Tree Oil.

In the present disclosure, grease a and grease b are different greases, wherein the letters a and b have no substantial meaning, but are only for convenience.

In the present disclosure, the blank hair bundle 1 and the blank hair bundle 2 are blank hair bundles from the same source, wherein the numbers 1 and 2 have no substantial meaning, but are only for convenience. In order to exclude the interference of the weight of the tress to the test results, the weight of the blank tress 1 and the blank tress 2 should be the same. The blank hair tresses 1 and 2 are preferably washed with a surfactant solution before use, mainly used to clean the dirt on the hair cuticle such as dust, dander, soot, grease, mineral oil, fatty acid, fatty alcohol, etc., suitable surfactant as described below.

In the present disclosure, the hair tress to be tested 1 and the tress to be tested 2 are the hair tresses to be tested obtained after being treated with grease for different times, and the operations such as scrubbing and drying are exactly the same, and the numbers 1 and 2 have no substantial meaning. Just for convenience. The hair strands to be tested obtained by treating with different oils for the same time are marked with the same reference numbers, for example, they are all marked as strands to be tested 1. It should be understood that the hair strands to be tested at this time are different due to the different types of oils to be treated, but their oils The processing time, scrubbing, drying, etc. are exactly the same.

In the present disclosure, permeable into the hair generally refers to permeable into the area of the hair cuticle near the hair cortex, the hair cortex and the medulla, especially the hair cortex and the medulla.

In the present disclosure, the method can be used to test whether the oil can penetrate into the hair, and can further judge the ability of the oil to penetrate into the hair. The peak signal intensity of the hair tress 2 to be tested treated with a treatment and the hair tress to be tested 2 treated with grease b at the comparison position, if the peak signal intensity of grease a is stronger than that of grease b, it is determined that the ability of grease a to penetrate into the hair is higher than that of grease a. Grease b is strong, and vice versa, it is judged that the penetrating ability of grease b is strong.

In step S1, the time for processing the blank hair bundle 1 is not more than 10 minutes, for example, not more than 9 minutes, 8 minutes, 7 minutes, and 6 minutes, preferably not more than 5 minutes, or even not more than 3 minutes and 1 minutes.

In step S2, the surfactant may be various surfactants commonly used in daily chemical products, including anionic surfactants, nonionic surfactants, amphoteric surfactants and cationic surfactants. The surfactant is preferably a surfactant with an HLB value of 9 or more, such as 9-13, 14-18, 19-25, 26-40.

Non-limiting examples of suitable surfactants include fatty alcohol sulfates such as sodium lauryl sulfate, triethanolamine lauryl sulfate, fatty alcohol polyoxyethylene ether sulfates such as sodium laureth sulfate, fatty Alcohol polyoxyethylene ether triethanolamine sulfate, sulfosuccinates such as disodium dodecyl sulfosuccinate, fatty alcohol polyoxyethylene ether sulfosuccinate monoester disodium, coconut oil monoethanolamide sulfosuccinic acid Monoester disodium, alkenyl sulfonate, N-acyl glutamate, alkyl phosphate (salt), fatty alcohol polyoxyethylene phosphonate, alkyl alcohol amide, fatty alcohol polyoxyethylene ether, alkane Phenol polyoxyethylene ether, ethylene oxide sorbitan monolaurate, betaine such as lauryl dimethyl betaine, cocamidopropyl betaine, hydroxyiodine betaine, ammonium oxide such as C12 -18 alkyl dimethyl amine oxide, lauryl amidopropyl amine oxide, N-alkyl amino propionate.

The surfactant solution generally refers to an aqueous solution of surfactant, and the concentration of the solution is not particularly limited.

In step S2, in order to better clean the oil on the surface of the tress, before rubbing the tress with the surfactant solution, it is preferable to wipe the surface of the tress with oil-absorbing paper.

In step S2, there is no special requirement for the water used for rinsing, and generally it can be tap water, deionized water, purified water, and distilled water. There are no special requirements for the drying operation, and it can be dried naturally.

In step S3, the time of soaking the tress to be tested with an organic solvent is preferably at least 10min, such as at least 15min, at least 30min, at least 45min, at least 1h, at least 1.5h, at least 2h, at least 2.5h, at least 3h, so that the hair inside The fat is dissolved into the organic solvent as much as possible.

Suitable organic solvents are organic solvents with a polarity parameter of less than 6, including but not limited to acetone, methyl isobutyl ketone, pyridine, n-propanol, isopropanol, n-butanol, isobutanol, ethyl acetate, butyl acetate Esters, dichloromethane, chloroform, carbon tetrachloride, benzene, toluene, n-pentane, isopentane, n-hexane, cyclohexane, n-heptane, isooctane, trimethylpentane, petroleum ether, diethyl ether , propyl ether, tetrahydrofuran.

The amount of the organic solvent is not particularly limited, as long as the hair strand to be tested can be completely immersed in the organic solvent. Even so, in order to prevent the fat content dissolved into the organic solvent from being too low, resulting in a weak H NMR signal of the subsequent test sample, it is advisable to immerse as many beams to be tested as possible in the organic solvent.

In step S3, the selection of the test solvent for NMR spectroscopy should satisfy the following conditions: ① it can dissolve the grease, ② it does not react with the grease, and ③ its resonance peak does not interfere with the signal of the grease. When the fat is vegetable oil, the test solvent is preferably not in the A hydrogen and B hydrogen of No. 1 carbon, the hydrogen of No. 2 carbon, and the A hydrogen and B hydrogen of No. 3 carbon as shown in formula (I) in the vegetable oil. The NMR peak position of at least one hydrogen in the vegetable oil is more preferably not in the NMR spectrum peak position of the B hydrogen of the No. 1 carbon or the B hydrogen of the No. 3 carbon as shown in formula (I):

Among them, the A hydrogen of carbon 1 and the A hydrogen of carbon 3 are in the same NMR peak position in many cases, the B hydrogen of carbon 1 and the B hydrogen of carbon 3 are in many cases the same NMR peak position, carbon 2 The position of the hydrogen NMR peak is different from that of the above-mentioned hydrogen.

In order to reduce the interference of hydrogen in the test solvent to the hydrogen in the grease during detection, the test solvent is preferably a deuterated organic solvent, suitably a deuterated organic solvent with a polarity parameter less than 6, preferably deuterated benzene, deuterated toluene , Deuterated chloroform.

In step S3, the organic solvent used for soaking the hair beam to be tested may be the same as or different from the test solvent used for hydrogen NMR spectroscopy. In a certain embodiment, the organic solvent is the same as the test solvent, and the organic solvent sample soaked in the hair beam to be measured is not treated, and is directly tested by hydrogen NMR. In another embodiment, the organic solvent is different from the test solvent. The organic solvent sample soaked in the hair beam to be tested is first dried to remove the organic solvent, and then the obtained substance is dissolved in the test solvent, and the NMR is carried out. Hydrogen spectrum test.

In step S3, the test conditions for the hydrogen NMR spectrum of the test solvent immersed in the blank hair bundle and the organic solvent immersed in the hair bundle to be tested, such as the magnetic field strength, the number of scans, the time for soaking the sample in the test solvent, the type of the test solvent, and the volume of the test sample They can be the same or different, preferably the same, so that when determining whether the oil can penetrate into the hair, especially the hair cortex and/or the medulla, the blank hair bundle's own signals and other information can not be interfered.

In step S4, step S2 is repeated until the hydrogen NMR spectrum measured in step S3 does not show a peak at the comparison position, and steps S2 and S3 can be repeated as a unit until the hydrogen NMR spectrum does not show a peak at the comparison position; S2 is repeated for one unit, and step S2 is repeated multiple times, such as repeated more than 3 times, and then step S3 is performed until no peak is found in the comparison position in the hydrogen nuclear magnetic spectrum.

In step S5, the time for processing the blank tress 2 is at least 10 minutes, such as at least 20 minutes, at least 30 minutes, at least 1 hour, at least 2 hours, preferably at least 3 hours, such as at least 5 hours, at least 8 hours, and at least 12 hours.

In step S5, the number of repetitions of rubbing the tress 2 is the same as the number of repetitions in step S4, if step S2 is not repeated in step S4, then the tress 2 is scrubbed once according to step S2, and if step S2 is repeated n times in step S4, then The tress 2 is scrubbed n+1 times according to step S2.

Description of drawings

Fig. 1a is the hydrogen nuclear magnetic spectrum of blank hair bundle in deuterated benzene, Fig. 1b is the hydrogen nuclear magnetic spectrum of coconut oil in deuterated benzene, Fig. 1c is the test beam 1 of Example 1 in deuterated benzene Hydrogen nuclear magnetic spectrum diagram, FIG. 1d is the hydrogen nuclear magnetic spectrum diagram of the beam 2 to be measured in Example 1 in deuterated benzene.

Fig. 2a is the hydrogen NMR spectrogram of argan oil in deuterated benzene, Fig. 2b is the hydrogen NMR spectrogram of the test beam 1 of Example 2 in deuterated benzene, Fig. 2c is the test beam 2 of Example 2 at H NMR spectrum in deuterated benzene.

Fig. 3 is the hydrogen nuclear magnetic spectrum diagram obtained by testing under the same conditions after the hair strand to be tested after being treated with the same method by coconut oil (top) and argan oil (bottom) is soaked in deuterated benzene for 3h.

FIG. 4a is a hydrogen nuclear magnetic spectrum diagram of the beam 1 to be measured in comparative example 1 in deuterated benzene, and FIG. 4b is a hydrogen nuclear magnetic spectrum diagram of the beam to be measured 2 of comparative example 1 in deuterated benzene.

Figure 5 is a schematic diagram of the diameter of the same position of hair after coconut oil treatment Oh (a), 1h (b), 2h (c), 16h (d) measured by microscope.

Detailed ways

The present invention is further described below by way of examples, but the present invention is not limited to the scope of the described examples. The experimental methods that do not specify specific conditions in the following examples are selected according to conventional methods and conditions, or according to the product description.

NMR test

Using Bruker's AscendTM 400 NMR instrument, BBO 400MHz S1 5mm with Z-gradientSP probe.

Microscope test

Performed with an Axio Scope A1 polarizing microscope from Carl Zeiss.

The substances involved in the following examples and comparative examples are all commercially available.

Blank tresses 1 and 2: Both are tresses that have not been treated with vegetable oil, and both come from the same source.

Example 1

The 1H NMR method to test whether coconut oil can penetrate into the hair involves the following steps:

S1. Take 2g of blank tress 1, apply 0.5mL of coconut oil evenly on it, and let it stand for 1min;

S2. Wipe and absorb the coconut oil on the surface of the hair tress with oil-absorbing paper, and then dropwise evenly add 0.25 mL of an aqueous solution of sodium laureth sulfate (2-3EO, HLB=30-40) with a concentration of 16 wt% to the hair tress, and rub for 30s , rinse with water for 30s; repeat the scrubbing steps of the above-mentioned sodium laureth sulfate aqueous solution, and dry the hair strands naturally;

S3. Weigh 90 mg of hair from the hair to be tested 1 dried in step S2, and immerse it into a sample bottle containing 3.6 mL of deuterated benzene. After soaking for 1 min, 10 min, 1 h, and 3 h, take 0.6 mL of hair from the sample bottle respectively. The sample is tested by hydrogen NMR; in this step, the hair beam to be tested is always completely immersed in deuterated benzene;

Weigh 90 mg of blank hair bundle 1 and immerse it into a sample bottle containing 3.6 mL of deuterated benzene. After soaking for 3 hours, take 0.6 mL of the sample from the sample bottle for hydrogen NMR spectroscopy. In this step, the blank hair bundle is always completely immersed in deuterium. in benzene;

Take 60mg of coconut oil and add it to the sample bottle containing 3.6mL of deuterated benzene. After the coconut oil is completely dissolved, take 0.6mL of the sample from the sample bottle for H NMR test;

S4. It is observed that the H NMR spectrum of blank hair bundle 1 (Fig. 1a) has no peaks around 4.2-4.3 ppm, while the H NMR spectrum of coconut oil (Fig. 1b) has peaks around 4.2-4.3 ppm, so 4.2- The position near 4.3ppm is set as the comparison position;

It is observed that the hydrogen nuclear magnetic spectrum (Fig. 1c) of the beam 1 to be measured does not show a peak at the comparison position, and then proceed to step S5;

S5. Take 2g of blank tress 2, apply 0.5 mL of coconut oil on it evenly, and let stand for 12 hours; wipe and absorb the coconut oil on the surface of the tress with oil-absorbing paper, and rub the tress 2 twice in the same way as in step S2. Air dry, and then use the same method of step S3 to carry out the hydrogen nuclear magnetic spectrum test of the hair beam 2 to be measured;

It is observed that the H NMR spectrum (Fig. 1d) of the hair bundle 2 to be tested shows a peak at the comparative position, it is determined that the coconut oil can penetrate into the hair.

In this embodiment, tresses 1 and 2 to be tested are all processed by coconut oil, but tresses to be tested 1 do not detect the signal of coconut oil, tress to be tested 2 detects the signal of coconut oil. This is because the coconut oil treatment time of the hair strand 1 to be tested is short, and the coconut oil only adheres to the surface of the hair and has not penetrated into the inside of the hair. No signal detected for coconut oil. And the hair tress 2 to be tested has a long treatment time with coconut oil, and the coconut oil not only adheres to the hair surface, but also penetrates into the inside of the hair. Although the coconut oil on the hair surface was cleaned by sodium laureth sulfate in step S5, it was not cleaned Coconut oil inside the hair, and subsequent coconut oil inside the hair dissolves into deuterated benzene, so the signal of coconut oil is detected.

Example 2

H NMR method to test whether argan oil penetrates into the hair.

The method steps of this embodiment are the same as those of embodiment 1, except that coconut oil is replaced by argan oil.

It was observed that the H NMR spectrum of argan oil (Fig. 2a) showed peaks around 4.2-4.3 ppm, while the H NMR spectrum of blank hair bundle 1 (Fig. 1a) did not show peaks around 4.2-4.3 ppm, so 4.2-4.3 ppm The nearby position is designated as the comparison position; the H NMR spectrum of the beam 1 to be tested (Fig. 2b) does not show a peak at the comparison position, and the H NMR spectrum of the beam 2 to be tested (Fig. 2c) has a peak at the comparison position, then it is determined that argan oil Penetrates into hair.

Example 3

H NMR method to test the ability of coconut oil and argan oil to penetrate into the hair.

Coconut oil in embodiment 1 and argan oil in embodiment 2 all measure that can penetrate into hair, and hair tress 2 to be tested is processed by coconut oil and argan oil in the same way and obtained and both the hydrogen NMR test conditions are the same , compare embodiment 1 to be measured hair bundle 2 and embodiment 2 to be tested hair bundle 2 after soaking in deuterated benzene for 3h, the peak signal intensity (Fig. 3) of the comparative position measured, it can be seen that the ratio of the ability of coconut oil to penetrate into hair Argan oil is strong.

Comparative Example 1

Same as Example 1, except that the following operations in steps S2 and S5 are different: after wiping and absorbing the coconut oil on the surfaces of the tresses 1 and 2 with oil-absorbing paper, the tresses 1 and 2 are immersed in 10 mL of toluene respectively, soaked for 1 min, and then used to absorb oil. The paper blotted the toluene on the surface of the hair, then put it into 10 mL of toluene, washed for 30s, took out the hair tress, and dried it naturally in a fume hood.

It was observed that the H NMR spectra of the tress 1 (Fig. 4a) and tress 2 (Fig. 4b) to be tested measured in step S3 both showed peaks at the comparative positions, because the toluene failed to remove the coconuts on the hair surface in step S2. After the oil was cleaned, the coconut oil on the hair surface of hair tress 1 to be tested and the coconut oil on the surface and inside of hair tress 2 to be tested were dissolved into deuterated benzene, so the signal of coconut oil was detected.

Comparative Example 2

The microscope test for coconut oil penetration into the hair involves the following steps:

S1. Take three blank hairs from the same source, fix them on glass slides respectively, and measure the initial diameters of the three blank hairs under microscope transmission mode;

S2. Soak three blank hairs in coconut oil for 1h, 2h and 16h respectively, observe and measure the diameter of the hair at the same position under the same mode;

S3, compare the hair diameter measured in step S2 and the initial diameter of step S1, if it is observed that the hair diameter after coconut oil treatment at different times is greater than the initial diameter, then it is judged that coconut oil can penetrate into the hair, otherwise it is judged that coconut oil cannot penetrate into the hair.

The specific test results are shown in Figure 5. It was not observed that the diameter of the hair treated with coconut oil at different times was significantly larger than the initial diameter. In theory, coconut oil can not only adhere to the surface of the hair, but also penetrate into the inside of the hair. The hair treated with coconut oil at different times will cause the hair diameter to become larger due to swelling. No penetration into the hair is not feasible.

Claims (14)

1. a test method, is characterized in that, comprises the steps: S1. Treat the blank tress 1 with grease a or b for no more than 10 minutes; S2, rub the treated hair tress with a surfactant solution, rinse with water, air dry, and wait for testing; S3, get the organic solvent immersed in the hair beam 1 to be tested and carry out the hydrogen NMR spectrum test, observe whether the obtained hydrogen NMR spectrum peaks at the comparison position, and the comparison position is located at the 1H NMR spectrum of the test solvent immersed in the blank hair beam 1. The peak area is located in the peak area of the hydrogen NMR spectrum of the grease a or b; the organic solvent for soaking the hair beam 1 to be measured and the test solvent for the hydrogen NMR spectrum are the solvents that can dissolve the grease a or b; S4. If the hair beam 1 to be measured does not show a peak at the comparison position, then go to step S5; if there is a peak, repeat step S2 until the H NMR spectrum measured in step S3 shows no peak at the comparison position, then go to step S5 ; S5. Treat the blank tress 2 with grease a or b for at least 10 min, and rub the tress 2 with the same method of step S2. 2; if the hair bundle 2 to be tested has a peak at the comparison position, it is determined that the oil a or b can penetrate into the hair, otherwise it is determined that the oil a or b cannot penetrate into the hair. 2. method as claimed in claim 1 is characterized in that, if grease a and b all can penetrate into hair in step S5, compare the tress to be tested 2 processed by grease a and the tress to be tested processed by grease b 2 When comparing the peak signal intensity at the position, if the peak signal intensity of oil a is stronger than that of oil b, it is judged that the ability of oil a to penetrate into the hair is stronger than that of oil b, and vice versa, it is determined that the penetration ability of oil b is strong. 3. The method of claim 1 or 2, wherein the permeable into the hair is permeation into the hair cortex and/or medulla of the hair. 4. The method of any one of claims 1-3, wherein the oil is vegetable oil. 5. The method according to any one of claims 1-4, wherein the time for processing the blank tress 1 in step S1 is not more than 5 minutes. 6. The method according to any one of claims 1-5, wherein in step S3, the time for soaking the hair bundle to be measured with an organic solvent is at least 10min. 7. The method according to any one of claims 1-6, wherein the time for processing the blank hair bundle 2 in step S5 is at least 3 hours. 8. the method as described in any one of claim 4-7 is characterized in that, described test solvent is not in the A hydrogen and B hydrogen of No. 1 carbon, the hydrogen of No. 2 carbon shown in vegetable oil as formula (I) , and the NMR peak position of at least one hydrogen in A hydrogen and B hydrogen of carbon 3:

9. The method of claim 8, wherein the test solvent does not appear at the NMR peak position of the B hydrogen of carbon No. 1 or the B hydrogen of carbon No. 3 of the vegetable oil as shown in formula (I). 10. The method according to any one of claims 1-9, wherein the organic solvent is the same as the test solvent, and the organic solvent sample soaked in the hair beam to be measured is untreated and directly subjected to hydrogen NMR spectroscopy test. 11. The method according to any one of claims 1-9, wherein the organic solvent is different from the test solvent, and the organic solvent sample soaked in the hair bundle to be tested is first dried to remove the organic solvent, Then, the obtained substance was dissolved in the test solvent, and the hydrogen NMR spectroscopy was carried out. 12. The method of any one of claims 1-11, wherein the test solvent is a deuterated organic solvent with a polarity parameter less than 6. 13. The method of any one of claims 1-12, wherein the deuterated organic solvent is selected from the group consisting of deuterated benzene, deuterated toluene and deuterated chloroform. 14. The method of any one of claims 1-13, wherein the HLB value of the surfactant is above 9.

Images