CN112098182A - Deep sea biological sample normal position chemistry fixing device - Google Patents

Abstract

The invention discloses an in-situ chemical fixing device for deep-sea biological samples. , the two ends of the rubber rope located in the bottle body are respectively connected with the inner sides of the two card caps, and the outer sides of the card caps are connected by a release unit; two fixing frames are arranged in the bottle body, and a mother liquor is arranged between the two fixing frames The fixing frame is provided with a bayonet limit hole, the bayonet limit holes of the two fixing frames are both provided with a bayonet, the pointed end of the bayonet is opposite to the mother liquor bag, and the blunt end of the bayonet extends to the opening of the bottle body place. The invention can be widely applied to various application scenarios, including the application in a high-pressure simulation chamber, has a simple structure, is convenient to use, and improves the efficiency of chemical fixation of biological samples.

Description

An in-situ chemical fixation device for deep-sea biological samples

technical field

The invention belongs to the technical field of deep-sea sampling equipment, in particular to an in-situ chemical fixation device for deep-sea biological samples, which can be applied to the collection and chemical fixation scenarios of biological samples in different water bodies.

Background technique

With the gradual refinement of life science exploration, it is usually necessary to detect the gene expression pattern or chromatin three-dimensional configuration information presented by biological samples in the original environment, which requires in situ chemical fixation while collecting samples. . In the past, the simple and direct sample collection method was to fish biological samples from the deep sea to the deck, and then add chemical reagents for fixation.

The main problems and deficiencies in the prior art include:

Since most of the deep-sea biological samples have been dead for a long time when they are caught to the sea surface, the gene expression in the cells and the three-dimensional structure of chromatin have been greatly changed, which makes it impossible to obtain relatively scientifically meaningful biological information. In recent years, there have been some technical solutions to release chemical fixatives through the extrusion of submersible manipulators. However, the number of submersible submersibles with a diving depth of more than 1000 meters and a manipulator is very limited, so the application of this solution is greatly limited. The high-pressure simulation chamber can simulate the high water pressure environment of the deep sea, and is often used to study the adaptability of deep-sea organisms to the high-pressure environment. In order to obtain scientifically meaningful biological information, in-situ chemical fixation devices are also required. With limited capacity, chemical fixtures that rely on robotic arms or rely on submersibles are clearly unusable.

The significance of solving the above technical problems:

There is a need for an in-situ chemical fixation device for deep-sea biological samples that can independently release fixatives, so that biological samples are collected in the original environment and also fixed by chemical reagents, maximize the retention of scientifically meaningful biological information, and can It is widely suitable for a variety of application scenarios, such as independent use, use in high-pressure chambers, use on water collection racks, and use on manned or unmanned submersibles.

SUMMARY OF THE INVENTION

In view of the above problems and deficiencies in the prior art, the present invention provides an in-situ chemical fixation device for deep-sea biological samples, which can be widely used in various application scenarios, including applications in high-pressure simulation chambers, with simple structure and convenient use. , which improves the efficiency of chemical fixation of biological samples.

For this reason, the present invention adopts the following technical solutions:

An in-situ chemical fixing device for deep-sea biological samples, comprising a bottle body, a clamping cover, a releaser unit and a fixing frame; the bottle body is open at both ends, and the clamping cover is located at the two openings of the bottle body and is located in the bottle body The two ends of the rubber rope are respectively connected with the inner sides of the two card covers, and the outside of the card covers are connected by a release unit; two fixing frames are arranged in the bottle body, and a mother liquid bag is arranged between the two fixing frames; the The fixing frame is provided with a bayonet limit hole, the bayonet limit holes of the two fixing frames are both provided with a bayonet, the pointed end of the bayonet is opposite to the mother liquor bag, and the blunt end of the bayonet extends to the opening of the bottle body.

Preferably, the snap cover includes a brim plate, one side of the brim plate is provided with an outer handle, the other side is provided with an inner plug, the inner plug is connected with the inner handle, the outer periphery of the inner plug is sleeved with an outer sealing ring, and the opening of the bottle body The inner wall is provided with an inner sealing ring adapted to the outer sealing ring; the outer handle is provided with an outer handle hole, the outer handle hole is connected with the release unit, the inner handle is provided with an inner handle hole, and the inner handle hole is connected with the rubber rope connection.

Preferably, the fixing frame includes two porous disks, the porous disks are connected by a support column, the porous disk is provided with a bayonet limit hole, and the positions of the bayonet limit holes of the two porous disks correspond one-to-one, The bayonet penetrates two bayonet limit holes corresponding to one-to-one positions on the two perforated discs, the two perforated discs are provided with long holes corresponding to the positions, and the mother liquid bag is provided with a mother liquid bag corresponding to the position of the long holes Through hole, the rubber rope passes through the elongated hole and the through hole of the mother liquid bag.

Preferably, a magnetic ring is fixed on the bayonet, and the magnetic ring is arranged on the side of the limiting hole of the bayonet facing the mother liquor bag.

Preferably, the porous disc is made of magnetically conductive material.

Preferably, the porous disc is stainless steel.

Preferably, the mother liquor bags include a plurality of them, which are arranged in parallel.

Preferably, the mother solution bag is a disposable product and adopts elastic material.

Preferably, the bottle side of the bottle body is provided with a water outlet nozzle, and the water outlet nozzle is blocked with a nozzle cap; the bottle side of the bottle body is also provided with a handle.

Preferably, the mother solution bag is provided with a fixative. After the mother solution bag is punctured by a bayonet, the fixative in the mother solution bag is mixed with the seawater in the bottle to form a fixative working solution, and the expected chemical reaction occurs with the biological sample to complete the biological In situ chemical fixation of samples.

Compared with the prior art, the beneficial effects of the present invention are:

(1) Relying on the interlocking transmission of the sample bottle's own release unit, bottle cap, built-in fixing frame, bayonet and mother liquid bag, the release of the fixative is realized, so that the device can independently complete the task of releasing the chemical fixative, so it can be widely used It can be used in a variety of application scenarios, including applications in high-pressure simulation chambers.

(2) The release of the fixative in the mother solution bag is realized by the impact of the bayonet on the cap, and the closure of the bottle body and the release of the fixative are almost synchronized, the transmission mechanism is simple, and the success rate is high; at the same time, a closed chamber is formed, which is beneficial to chemical reagents Play a role.

(3) Relying on the rupture of the mother liquid bag to release the fixative, the opening is huge and the release speed is fast, which is conducive to the realization of fast in-situ fixation.

(4) The fixative is stored in the mother liquid bag of soft material, which is conducive to the discharge of air in advance, and is more suitable for deep sea high pressure environment.

(5) Multiple mother liquid bags can be used in parallel, which can avoid early reaction between certain chemical reagents and is conducive to preserving the activity of chemical reagents.

(6) The mother liquid bag is made of elastic material. After the destruction of the bayonet, the crack is larger and the reagent is released more quickly.

(7) The mother liquid bag is a disposable product, which can avoid mutual contamination.

(8) The fixing frame can not only be used to limit the sliding direction of the bayonet, but also be used to fix the bait, attract the aggregation of organisms, and improve the efficiency of chemical fixation of biological samples.

Description of drawings

FIG. 1 is a schematic view of the structure of a right side view of an in-situ chemical fixation device for a deep-sea biological sample provided by the present invention.

FIG. 2 is a schematic top-view structural diagram of a deep-sea biological sample in-situ chemical fixation device provided by the present invention.

Figure 3 is a schematic view of the development of the bottle body.

Figure 4 is a schematic diagram of the structure of the bayonet.

FIG. 5 is a schematic view of the structure of the fixing frame.

Description of reference numerals: 1, bottle body; 2, snap cover; 3, release unit; 4, fixing frame; 201, brim plate; 202, inner plug; 203, inner handle; 204, inner handle hole; 205, rubber rope; 206, outer sealing ring; 207, outer handle; 208, outer handle hole; 401, porous disc; 402, support column; 403, bayonet limit hole; 404, bayonet; 405, long hole; 406, magnetic ring ; 407, mother liquid bag; 408, mother liquid bag through hole; 4041, blunt end; 4042, pointed end.

Detailed ways

The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments, wherein the specific embodiments and descriptions are only used to explain the present invention, but are not intended to limit the present invention.

As shown in Figures 1 and 2, the present invention discloses an in-situ chemical fixing device for deep-sea biological samples, comprising a bottle body 1, a snap cover 2, a releaser unit 3 and a fixing frame 4; the two ends of the bottle body 1 The card cover 2 is located at the two openings of the bottle body 1, the two ends of the rubber rope 205 located in the bottle body 1 are respectively connected with the inner sides of the two card covers 2, and the outside of the card covers 2 passes through the release unit 3. connection; two fixing frames 4 are arranged in the bottle body 1, and a mother liquor bag 407 is arranged between the two fixing frames 4; Each of the limiting holes 403 is provided with a bayonet 404 , the pointed end 4042 of the bayonet 404 is opposite to the mother liquor bag 407 , and the blunt end 4041 of the bayonet 404 extends to the opening of the bottle body 1 .

Specifically, the card cover 2 includes a brim plate 201, one side of the brim plate 201 is provided with an outer handle 207, the other side is provided with an inner plug 202, the inner plug 202 is connected with the inner handle 203, and the outer circumference of the inner plug 202 is sleeved with a The outer sealing ring 206, the inner wall of the opening of the bottle body 1 is provided with an inner sealing ring adapted to the outer sealing ring 206; the outer handle 207 is provided with an outer handle hole 208, and the outer handle hole 208 is connected with the release unit 3, so The inner handle 203 is provided with an inner handle hole 204 , and the inner handle hole 204 is connected with the rubber cord 205 .

Specifically, as shown in FIG. 3 and FIG. 5 , the fixing frame 4 includes two porous discs 401, the porous discs 401 are connected by a support column 402, and a bayonet limiting hole 403 is provided on the porous disc 401, The positions of the bayonet limit holes 403 of the two porous discs 401 correspond one-to-one, the bayonet 404 penetrates the two bayonet limit holes 403 corresponding to the positions of the two porous discs 401 , and the two porous discs 401 are provided with The position corresponding to the elongated hole 405, the mother solution bag 407 is provided with a mother solution bag through hole 408 corresponding to the position of the elongated hole 405, and the rubber rope 205 passes through the elongated hole 405 and the mother solution bag through hole 408.

Specifically, as shown in FIG. 4 , a magnetic ring 406 is fixed on the bayonet 404 , and the magnetic ring 406 is arranged on the side of the bayonet limiting hole 403 facing the mother solution bag 407 .

Specifically, the porous disk 401 is made of a magnetic conductive material.

Specifically, the porous disc 401 is stainless steel.

Specifically, the mother solution bags 407 include a plurality of them, which are arranged in parallel.

Specifically, the mother liquid bag 407 is a disposable product, and is made of elastic material.

Specifically, the bottle side of the bottle body 1 is provided with a water outlet nozzle, and the water outlet nozzle is blocked with a nozzle cap; the bottle side of the bottle body 1 is also provided with a handle.

Specifically, the mother solution bag 407 is provided with a fixative. After the mother solution bag 407 is punctured by the bayonet 404, the fixative in the mother solution bag 407 is mixed with the seawater in the bottle body 1 to form a fixative working solution, which is expected to occur with biological samples. chemical reaction to complete the in situ chemical immobilization of biological samples.

Example

An in-situ chemical fixing device for deep-sea biological samples, comprising a bottle body 1 with openings at both ends, and a snap cover 2 arranged at the two openings of the bottle body 1, and two ends of a rubber rope 205 located in the bottle body 1 are respectively Connect with the inside of the two card covers 2.

Two fixing frames 4 are arranged in the bottle body 1, a mother liquor bag 407 is arranged between the two fixing frames 4, and a bayonet limiting hole 403 is arranged on the fixing frame 4. A bayonet 404 is provided, the pointed end 4042 of the bayonet 404 is opposite to the mother liquid bag 407, and the blunt end 4041 of the bayonet 404 extends to the opening of the bottle body 1; on the holder 4.

When the cap 2 is closed, the cap 2 hits the blunt end 4041 of the bayonet 404, and the bayonet 404 slides along the bayonet limiting hole 403 to the middle part of the bottle body 1, so that the pointed end 4042 of the bayonet 404 penetrates into the bottle body. 1. The mother solution bag 407 in the middle position breaks the mother solution bag 407, and the fixative in the mother solution bag 407 is rapidly released into the bottle body 1 through the break, and is mixed with the seawater in the bottle body 1 to form a fixative working solution, thereby The expected chemical reaction occurs with the biological sample, and the in-situ chemical immobilization of the biological sample is completed.

The fixing frame 4 includes two porous discs 401, the porous discs 401 are connected by a support column 402, a bayonet limiting hole 403 is provided on the porous disc 401, and the bayonet limiting holes 403 of the two porous discs 401 are located at the same position. Correspondingly, the bayonet 404 penetrates the two bayonet limiting holes 403 on the two perforated discs 401 in one-to-one correspondence, the two perforated discs 401 are provided with elongated holes 405 corresponding to their positions, and the mother solution bag 407 is provided with The mother solution bag through-hole 408 corresponding to the position of the elongated hole 405, the rubber rope 205 passes through the elongated hole 405 and the mother solution bag through-hole 408.

A magnetic ring 406 is fixed on the bayonet 404 , the porous disc 401 is made of a magnetically conductive material, and the magnetic ring 406 is arranged on the side of the bayonet limiting hole 403 facing the mother liquid bag 407 .

The bayonet limit hole 403 has a limiting effect on the bayonet 404. The bayonet 404 can only move along the axial direction of the bayonet limit hole 403, so that when the card cover 2 hits the blunt end 4041 of the bayonet 404, the bayonet 404 moves along the bayonet limit hole. When the 403 moves in the axial direction, the pointed end 4042 of the bayonet 404 will pierce the mother solution bag 407 . When the bayonet 404 is not hit by the card cover 2 , the bayonet 404 can maintain the relative positional relationship with the fixing frame 4 through the magnetic attraction between the magnetic ring 406 and the inner wall of the bayonet limiting hole 403 . After the bayonet 404 is hit by the card cover 2 , the impact force is greater than the magnetic attraction force, and the pointed end 4042 of the bayonet 404 pierces the mother solution bag 407 .

Depending on the actual situation, the use methods are also different, and can be divided into three different application forms: manual form, timing form and trigger form.

The manual form means that the releaser unit 3 is connected to the control module of the ship or shore or manned submersible, and the in-situ chemical fixation of the biological sample is realized by manually controlling the releaser unit 3 .

The timing form means that by writing a time control program in the releaser unit 3 in advance, it works at the set time, closes the card cover 2, and releases the chemical fixative. If some organisms have accumulated in the bottle body 1 at this time, in-situ chemical immobilization can be achieved. This form of use is relatively independent, and can also be used in high-voltage simulation chambers.

The trigger form refers to the specific signal received by the sensor in the releaser unit 3, such as the signal that the organism enters the bottle body 1, etc., the operation of the releaser unit 3 is automatically triggered, so as to realize the in-situ chemical immobilization of the biological sample.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and scope of the present invention shall be included in the scope of the present invention. within the scope of protection.

Claims (10)

1. The in-situ chemical fixing device for the deep sea biological sample is characterized in that: comprises a bottle body (1), a clamping cover (2), a releaser unit (3) and a fixed frame (4); the two ends of the bottle body (1) are opened, the clamping covers (2) are positioned at the two openings of the bottle body (1), the two ends of the rubber rope (205) positioned in the bottle body (1) are respectively connected with the inner sides of the two clamping covers (2), and the outer sides of the clamping covers (2) are connected through the releaser unit (3); two fixing frames (4) are arranged in the bottle body (1), and a mother liquid bag (407) is arranged between the two fixing frames (4); the bottle is characterized in that the fixing frames (4) are provided with bayonet limiting holes (403), the bayonet limiting holes (403) of the two fixing frames (4) are internally provided with the bayonet (404), the pointed end (4042) of the bayonet (404) is opposite to the mother liquor bag (407), and the blunt end (4041) of the bayonet (404) extends to the opening of the bottle body (1).

2. The deep sea biological sample in-situ chemical fixing device according to claim 1, characterized in that: the clamping cover (2) comprises a brim plate (201), one side surface of the brim plate (201) is provided with an outer handle (207), the other side surface of the brim plate is provided with an inner plug (202), the inner plug (202) is connected with the inner handle (203), the outer periphery of the inner plug (202) is sleeved with an outer sealing ring (206), and the inner wall of an opening of the bottle body (1) is provided with an inner sealing ring matched with the outer sealing ring (206); an outer handle hole (208) is formed in the outer handle (207), the outer handle hole (208) is connected with the releaser unit (3), an inner handle hole (204) is formed in the inner handle (203), and the inner handle hole (204) is connected with the rubber rope (205).

3. The deep sea biological sample in-situ chemical fixing device according to claim 1, characterized in that: the fixing frame (4) comprises two porous discs (401), the porous discs (401) are connected through a support column (402), the porous discs (401) are provided with bayonet limiting holes (403), the bayonet limiting holes (403) of the two porous discs (401) are in one-to-one correspondence with each other in position, the bayonet (404) penetrates through the two bayonet limiting holes (403) in the two porous discs (401) in one-to-one correspondence with each other in position, the two porous discs (401) are provided with elongated holes (405) in corresponding positions, mother liquor bag through holes (408) corresponding to the elongated holes (405) in position are formed in the mother liquor bag (407), and the rubber rope (205) penetrates through the elongated holes (405) and the mother liquor bag through holes (408).

4. The deep sea biological sample in-situ chemical fixing device according to claim 3, characterized in that: a magnetic ring (406) is fixed on the bayonet (404), and the magnetic ring (406) is arranged on one side of the bayonet limiting hole (403) facing the mother liquid bag (407).

5. The deep sea biological sample in-situ chemical fixing device according to claim 4, characterized in that: the porous disc (401) is made of magnetic conductive materials.

6. The deep sea biological sample in-situ chemical fixing device according to claim 5, characterized in that: the porous disc (401) is stainless steel.

7. The deep sea biological sample in-situ chemical fixing device according to claim 1, characterized in that: the mother liquid bag (407) comprises a plurality of mother liquid bags which are arranged in a parallel mode.

8. The deep sea biological sample in-situ chemical fixing device according to claim 1, characterized in that: the mother liquor bag (407) is a disposable article and is made of elastic materials.

9. The deep sea biological sample in situ chemical fixing device according to any one of claims 1 to 8, characterized in that: a water outlet nozzle is arranged at the bottle side of the bottle body (1), and a nozzle cap is arranged on the water outlet nozzle in a plugging manner; the bottle side of the bottle body (1) is also provided with a handle.

10. The deep sea biological sample in situ chemical fixing device according to any one of claims 1 to 8, characterized in that: a fixing agent is arranged in the mother liquid bag (407), the fixing agent in the mother liquid bag (407) is mixed with seawater in the bottle body (1) after the mother liquid bag (407) is punctured by the puncture knife (404) to form a fixing agent working solution, and the fixing agent working solution and the biological sample undergo an expected chemical reaction to complete the in-situ chemical fixation of the biological sample.

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