CN112088979A - Mulberry leaf preservative and preparation method and application thereof - Google Patents

Abstract

The invention provides a mulberry leaf preservative, a preparation method and application thereof, and belongs to the technical field of mulberry leaf storage and preservation. In the mulberry leaf preservative provided by the invention, the effective ingredient is garlic, and the mass percentage of garlic in the mulberry leaf preservative is 5% to 10%. The results of the examples show that: using the preservative of the present invention to preserve the mulberry leaves, after 7 days of storage, the water loss rate of the mulberry leaves is within 10%, and the mulberry leaves treated with the preservative of the present invention are fed to silkworms, which promotes the growth of silkworms. Growth and development, significantly increased the cocoon production of silkworms and reduced the dead cage rate. The mulberry leaf preservative provided by the invention not only achieves the expected effect of mulberry leaf fresh-keeping, but also ensures the safety of silkworm farming and can achieve the effect of increasing silkworm cocoon production, and provides a scientific and technological theory for realizing labor-saving, standardization, scale and intensification of planting mulberry and raising silkworms. basis and technical support.

Description

A kind of mulberry leaf preservative and its preparation method and application

technical field

The invention belongs to the technical field of mulberry leaf storage and preservation, in particular to a mulberry leaf preservative and a preparation method and application thereof.

Background technique

With the development of mulberry and sericulture industry, the silage preservation technology of mulberry leaves has been paid more and more attention. At present, the commonly used mulberry leaf preservatives are mostly chemical reagents, which are unsafe for silkworms and poisonous to silkworms; only spraying water on mulberry leaves for fresh-keeping will shorten the fresh-keeping time, and the mulberry leaves are easily mildewed and deteriorated, which induces silkworm disease and reduces yield. Cocoon volume. Therefore, there is an urgent need for a preservative that is non-toxic to silkworms and has a long fresh-keeping period.

SUMMARY OF THE INVENTION

In order to solve the above problems, the present invention provides a mulberry leaf preservative and its preparation method and application. The mulberry leaf preservative provided by the invention not only has a longer mulberry leaf fresh-keeping effect, but also ensures the safety of silkworm farming and can achieve the effect of increasing the production of silkworm cocoons. Theoretical basis and scientific and technological support.

In order to achieve the above object, the present invention provides the following technical solutions:

The invention provides a mulberry leaf preservative. The effective ingredient is garlic, and the mass percentage of garlic in the mulberry leaf preservative is 5% to 10%.

Preferably, the active ingredient of the mulberry leaf preservative further includes white granulated sugar, and the mass percentage of white granulated sugar in the mulberry leaf preservative is 5% to 10%.

Preferably, the mass percentage content of garlic in the mulberry leaf preservative is 5%, and the mass volume percentage content of the white granulated sugar is 5%.

The present invention provides the preparation method of the mulberry leaf preservative in the above technical scheme, which includes the following steps: pulverizing the garlic raw material and then mixing it into water to obtain the mulberry leaf preservative.

Preferably, when the mulberry leaf preservative includes white granulated sugar, the white granulated sugar and the crushed garlic are mixed into water to obtain the mulberry leaf preservative.

Preferably, the mixing time is 5-10 min; after the mixing, it also includes clarifying the mixed solution for 1-2 hours to obtain the mulberry leaf preservative.

The present invention provides a method for using the mulberry leaf preservative in the above technical scheme, comprising the following steps: spraying the mulberry leaf preservative on the mulberry leaves, storing the mulberry leaves after spraying the preservative, and turning the mulberry leaf every day during the storage period Mulberry leaves.

Preferably, the dosage of the mulberry leaf preservative per kilogram of mulberry leaves is 0.15-0.25 kg; the humidity of the storage is 75%-90%; the temperature of the storage is 20-28°C.

Preferably, the mulberry leaves after spraying the preservative are covered with a plastic film with holes, the diameter of the holes is 1-2 mm, and the density of the holes is 30-50/m ² .

The invention provides a method for increasing the production of silkworm cocoons, comprising the following steps: feeding the mulberry leaves treated with the mulberry leaf preservative described in the above technical solution.

Beneficial effects:

The invention provides a mulberry leaf preservative. The effective ingredient is garlic, and the mass percentage of garlic in the mulberry leaf preservative is 5% to 10%. The mulberry leaf preservative provided by the invention not only achieves the expected effect of mulberry leaf fresh-keeping, but also ensures the safety of silkworm farming and can achieve the effect of increasing silkworm cocoon production, and provides a scientific and technological theory for realizing labor-saving, standardization, scale and intensification of planting mulberry and raising silkworms. basis and technical support. The results of the examples show that eating the mulberry leaves treated with the mulberry leaf preservative of the present invention can promote the growth and development of silkworms, the fifth instar is shortened by 12 to 30 hours, the full age is shortened by 6 to 30 hours, and the cocooning rate is increased by 0.39% to 1.38%. The dead cage rate is reduced by 0.11% to 0.48%, the total cocoon volume, cocoon layer volume and cocoon layer rate are increased by 2.02% to 10.23%, 1.90% to 15.22%, 0.81% to 4.48%, and the production of cocoons by Zhang seeds is increased by 4.91% to 4.91%. 15.42%; the number of eggs laid by single moth was increased by 8.23% to 18.78%.

Detailed ways

The invention provides a mulberry leaf preservative. The effective ingredient is garlic, and the mass percentage of garlic in the mulberry leaf preservative is 5% to 10%. In the present invention, the active ingredient of the mulberry leaf preservative is garlic, which has the effect of disinfection and sterilization, and has the functions of inhibiting and killing pathogenic microorganisms such as bacteria, fungi and viruses. At the same time, it can promote the silkworm body. Growth and development, increase the amount of cocoons produced by silkworms, and reduce the dead cage rate. The garlic of the present invention is preferably a garlic clove without pollution and mildew. The present invention has no special requirements on the source of garlic, and ordinary commercially available garlic is sufficient. In the present invention, the mass percentage content of garlic in the mulberry leaf preservative is more preferably 5%. In the present invention, the solvent of the mulberry leaf preservative is preferably water. The water is preferably boiled water or drinking barreled mineral water, so as to prevent the use of natural water with viruses or bacteria from affecting the fresh-keeping effect of mulberry leaves. In the present invention, the active ingredient of the mulberry leaf preservative also preferably includes white sugar, and the mass percentage of white sugar in the mulberry leaf preservative is preferably 5% to 10%; when the mulberry leaf preservative is used In the spring silkworm stage, the mass percentage content of the white sugar is more preferably 8% to 10%, more preferably 10%; when the mulberry leaf preservative is used in the summer and autumn silkworm stage, the quality of the white sugar is The percentage content is more preferably 5% to 8%, and still more preferably 5%. In the present invention, when the mulberry leaf preservative includes garlic and white sugar, the mass percentage content of garlic in the mulberry leaf preservative is preferably 5% to 10%, more preferably 5%; The percentage content is preferably 5% to 10%, more preferably 5%. The effect of extending the fresh-keeping period of white sugar plants is better than that of light salt water, liquid flower fertilizer and water. Adding food has the effect of supplementing heat and improving blood circulation in animal feed. In the present invention, adding white sugar to the mulberry leaf preservative can further promote the growth and development of silkworms and improve the yield and quality of silkworm cocoons.

The mulberry leaf preservative of the invention has good fresh-keeping effect, can ensure the safety of silkworm farming and can achieve the effect of increasing silkworm cocoon production, and provides scientific and technological theoretical basis and scientific and technological support for realizing labor-saving, standardization, scale and intensification of planting mulberry and raising silkworms .

The present invention provides the preparation method of the mulberry leaf preservative in the above technical scheme, which comprises the following steps: pulverizing garlic raw materials and mixing them into water to obtain the mulberry leaf preservative. In the present invention, the garlic raw materials are crushed and mixed into water. In the present invention, the particle size of the crushed garlic clove raw material is preferably 3-5 mm, more preferably 3 mm. In the present invention, when the mulberry leaf preservative includes white granulated sugar, the white granulated sugar and the crushed garlic are mixed into water to obtain the mulberry leaf preservative. The present invention does not have any special requirements on the mixing method, and the mixing can be done by a method known to those skilled in the art. In the present invention, stirring is preferred for mixing, and the stirring time is preferably 5 to 10 min, more preferably 10 min. After mixing, the present invention also includes clarifying the mixed solution for 1-2 hours, more preferably 1.5 hours; in the present invention, the clarification is preferably sealed and clarified, which can prevent the volatilization of allicin nutrients; after clarification, the Mulberry leaf preservative.

The present invention provides a method for using the mulberry leaf preservative in the above technical scheme, comprising the following steps: spraying the mulberry leaf preservative on the mulberry leaves, storing the mulberry leaves after spraying the preservative, and turning the mulberry leaf every day during the storage period Mulberry leaves.

In the present invention, the mulberry leaf preservative is sprayed on the mulberry leaf. In the present invention, the dosage of the mulberry leaf preservative per kilogram of mulberry leaves is preferably 0.15 to 0.25 kg, more preferably 0.2 kg, and it is advisable that the mulberry leaves do not drip water, which can not only achieve good disinfection and sterilization effects, but also will not be wasted. After spraying on the mulberry leaves, the present invention preferably covers the mulberry leaves after spraying the preservative with a perforated plastic film, and the diameter of the hole is preferably 1-2 mm, more preferably 1 mm; The density is preferably 30 to 50 pieces/m ² , more preferably 40 pieces/m ² . It is covered with a perforated plastic film to facilitate moisturizing and keep the mulberry leaves fresh; it can also isolate the poisonous dust in the air from contaminating the mulberry leaves; it can also keep the stored mulberry leaves well ventilated to prevent scorching fermentation and deterioration.

After spraying the mulberry leaf preservative of the present invention, the present invention preferably stores the mulberry leaves after spraying the preservative. The present invention is preferably stored in a low temperature and humid environment. In the present invention, the humidity of the storage is preferably 75% to 90%, more preferably 75% to 80%, and even more preferably 80%; the temperature of the storage is preferably 20 to 28°C, more preferably 20 to 24°C, more preferably 20°C. The storage conditions of the invention are suitable, bacteria are not easy to breed, and the fresh-keeping effect is the best. In the present invention, it is preferable to turn over once a day during fresh-keeping storage to prevent accumulation and burning.

The invention provides a method for increasing the production of silkworm cocoons, comprising the following steps: feeding the mulberry leaves treated with the mulberry leaf preservative described in the above technical solution.

In order to further illustrate the present invention, a kind of disinfectant for silkworms provided by the present invention and its using method are described in detail below in conjunction with the examples, but they should not be construed as limiting the protection scope of the present invention.

Example 1

A mulberry leaf preservative, the effective component is garlic, and the mass percentage of garlic is 5%.

Preparation method: Peel commercially available garlic, select non-polluting and mildew-free garlic cloves; wash the garlic cloves with clean water, and dry them; pulverize the dried garlic cloves, and the particle size after pulverization is 4 mm; The boiling water of 100 ml is used as the solvent, and the crushed garlic is added to 100 ml of water, stirred for 10 minutes, after stirring evenly, sealed and clarified for 1.5 hours, and the clear liquid can be taken.

How to use: Spray the mulberry leaf preservative on the mulberry leaves. The amount of the mulberry leaf preservative per kilogram of mulberry leaves is 0.2kg. Use a plastic film with holes to cover the mulberry leaves and cushion them. The diameter of the holes is 1mm. , the density of the holes is 40/m ² ; the mulberry leaves after spraying the preservative are stored in a low temperature, humid and clean storage room, the temperature of the storage room is 25 ℃, and the relative humidity is 80%; during the storage period, the mulberry leaves are turned once a day, Prevent accumulation of heat.

Example 2

A mulberry leaf preservative, the effective component is garlic, and the mass percentage of garlic is 10%.

The preparation method and use method are the same as those in Example 1.

Example 3

A mulberry leaf preservative, the effective component is garlic, and the mulberry leaf preservative also contains white sugar, wherein the mass percentage of garlic is 5%, and the mass percentage of white sugar is 5%.

Preparation method: commercially available garlic and white sugar; peel the commercially available garlic, and select non-polluting and mildew-free garlic cloves; wash the garlic cloves with water, and dry them; crush the garlic cloves, and the particle size after crushing is 4 mm; The cooled boiled water is used as a solvent, the crushed garlic is added to the water solvent, then white granulated sugar is added, stirred for 10 minutes, stirred evenly, clarified for 1.5 hours, and then the clear liquid can be taken.

The use method is the same as that of Example 1.

Example 4

A mulberry leaf preservative, the effective component is garlic, the mulberry leaf preservative also contains white sugar, wherein the mass percentage of garlic is 5%, and the mass percentage of white sugar is 10%.

Preparation method: commercially available garlic and white sugar; peel the commercially available garlic, and select non-polluting and mildew-free garlic cloves; wash the garlic cloves with water, and dry them; crush the garlic cloves, and the particle size after crushing is 4 mm; The cooled boiled water is used as a solvent, the crushed garlic is added to the water solvent, then white granulated sugar is added, stirred for 10 minutes, stirred evenly, clarified for 1.5 hours, and then the clear liquid can be taken.

The use method is the same as that of Example 1.

Example 5

A mulberry leaf preservative, the effective component is garlic, the mulberry leaf preservative also contains white sugar, wherein the mass percentage of garlic is 10%, and the mass percentage of white sugar is 5%.

Preparation method: commercially available garlic and white sugar; peel the commercially available garlic, and select non-polluting and mildew-free garlic cloves; wash the garlic cloves with water, and dry them; crush the garlic cloves, and the particle size after crushing is 4 mm; The cooled boiled water is used as a solvent, the crushed garlic is added to the water solvent, then white granulated sugar is added, stirred for 10 minutes, stirred evenly, clarified for 1.5 hours, and then the clear liquid can be taken.

The use method is the same as that of Example 1.

Comparative Example 1

Good preservation: Dilute the water solution with good preservation (Linyi Denong Biotechnology Co., Ltd.) 1000 times.

Usage: Same as Example 1.

Comparative Example 2

Duolingo preservative: an aqueous solution of 90% Duolingo water dispersible granules (Anhui Guangxin Agrochemical Co., Ltd.) diluted 1000 times.

Usage: Same as Example 1.

Comparative Example 3

Thiophanate-methyl preservative: an aqueous solution of 70% thiophanate-methyl wettable powder (Shandong Bainong Star Biotechnology Co., Ltd.) diluted 1000 times.

Usage: Same as Example 1.

Comparative Example 4

EM active bacteria (produced by Linyi Kangbaoxing Biological Products Co., Ltd.) were diluted into an aqueous solution of 1000 times.

Usage: Same as Example 1.

Application example 1

Effects of Mulberry Leaf Preservatives on Water Loss Rate and Mildew of Mulberry Leaves in Storage

In September 2019, the mulberry leaf preservation test was carried out in the Zunyi Base Laboratory of the Sericulture Research Institute of the Guizhou Academy of Agricultural Sciences. Under the conditions of a room temperature of 26 °C and a relative humidity of 80%, 8 treatment areas were set up, and each treatment set 3 In the repeating area, the weight of mulberry leaves in each repeating area is 1kg, and the dosage of mulberry leaves per kilogram of mulberry leaves is 0.2kg. The mulberry variety tested was Nongsang No. 14, and the mulberry leaves were the mature leaves of 3-5 instar silkworms with the same maturity.

Treatment A: Use the fresh-keeping agent of Comparative Example 1 as a preservative;

Treatment B: use the 90% doxycycline water dispersible granules of Comparative Example 2 as a preservative;

Treatment C: use the 70% thiophanate-methyl wettable powder of Comparative Example 3 as a preservative;

Process D: use the mulberry leaf preservative of Example 1 to keep fresh;

Process E: use the mulberry leaf preservative of Example 2 to keep fresh;

Process F: use the mulberry leaf preservative of Example 3 to keep fresh;

Treatment G: use the EM active bacteria of Comparative Example 4 as a preservative;

Treatment CK1: treated with water to keep fresh as control area 1;

Treatment CK2: routinely covered with plastic film and kept fresh as control area 2.

Weigh 1kg of mulberry leaves for each repetition of each test, weigh the weight after processing, and use it as the base number, and then investigate the weight and water loss rate of mulberry leaves every day. See Table 1, Table 2, and Table 3 in turn.

Table 1 Effects of different preservatives on water loss rate of mulberry leaves

It can be seen from Table 1 that under the conditions of temperature of 26°C and relative humidity of 80%, different medicines as preservatives have certain influence on the water loss weight and water loss rate of mulberry leaves. With the prolonged storage time, the water loss rate of mulberry leaves is high. On the 7th day of storage, the order of the water loss rate from small to large was treatment F < treatment D < treatment E < treatment B < treatment G < treatment A < treatment C < treatment CK1 < treatment CK2. The water loss rates of treatments F, D, E, B and G were 3.30%, 4.35%, 6.96%, 8.70% and 9.57% respectively, and the preservative of the present invention had better preservation effect.

Table 2 Mildew condition of mulberry leaves by different preservatives

deal with September 1 September 3 September 5 September 6 September 7 September 9 September 11 September 13 A no no no no no no no no B no no no no no no no Yes (5.6%) C no no no no no no no Yes (10.21%) D no no no no no Yes (3.2%) Yes (5.3%) Yes (15%) E no no no no no no Yes (4.1%) Yes (12.8%) F no no no no no Yes (2.8%) Yes (4.5%%) Yes (18%) G no no no no Yes (3.1%) No (6.5%) yes (15.4) No (28.6%) Control 1 no no no Yes (4.5%) Yes (10%) Yes (25%) Yes (50%) Yes (65%) Control 2 no no Yes (8%) Yes (15%) Yes (40%) Yes (65%) Yes (100%) Yes (100%)

Remarks: Mildew refers to the phenomenon of mildew or rot at the base of leaves or petioles.

It can be seen from Table 2 that under certain temperature and humidity conditions, different medicines are used as preservatives, and the mildew time and mildew degree of mulberry leaf storage are also different. As the storage time is longer, the degree of mildew damage is more. The order of the degree of mildew-induced damage from mild to severe is: treatment A < treatment B < treatment C < treatment E < treatment D < treatment F < treatment G < treatment CK1 < treatment CK2, the preservative of the present invention has good mold prevention and control The effect of mulberry leaf storage for seven days did not occur mildew, which was better than the anti-mildew effect of EM bacteria, water and film.

Table 3 Poisoning investigation of silkworm after eating

deal with A B C D E F G CK1 CK2 first instar silkworm 1 1 1 0 0 0 0 0 0 Second instar silkworm 1 1 1 0 0 0 0 0 0 three silkworms 1 1 1 0 0 0 0 0 0 four-year-old silkworm 1 1 1 0 0 0 0 0 0 Five-year-old silkworm 1 1 1 0 0 0 0 0 0

Remarks: non-toxic (0), toxic (1)

As can be seen from Table 3, processing A, B, C is used as a preservative, and after the silkworms eat, they are poisoned and die, which is unsafe to silkworms. for safety.

To sum up, the mulberry leaf preservative of the present invention has a relatively low water loss rate of mulberry leaves, within seven days, the water loss rate is within 10%, and the phenomenon of mildew has not yet occurred, which is safe and non-toxic to silkworm farming.

Application example 2

In September 2019, in the Zunyi Base Laboratory of the Sericulture Research Institute of the Guizhou Academy of Agricultural Sciences, the effects of silkworm feeding on the mulberry leaves treated with the mulberry leaf preservative of the present invention were carried out on their growth, development and economic traits. 5 treatment areas were set up, and each treatment was set up with 3 repeating areas. In each repeating area, 400 silkworms were randomly selected for the experiment until the fourth instar silkworms were fed for the second time. The mulberry leaf variety tested was Nongsang No. 14, and the silkworm variety was Huakang No. 2. Treatment K: the mulberry leaves treated with the mulberry leaf preservative of Example 1 were used to raise silkworms; Treatment J: the mulberry leaves treated with the mulberry leaf preservative of Example 2 were used to raise silkworms; Treatment L: the mulberry leaf preservatives of Example 3 were used to raise silkworms The treated mulberry leaves were raised for silkworms; Treatment M: the silkworms were raised with the mulberry leaves treated with the mulberry leaf preservative of Example 4; Treatment N: the silkworms were raised with the mulberry leaves treated with the mulberry leaf preservatives of Example 5; CK1: fresh-keeping with water CK2: Mulberry leaves are often stored and raised, and the growth and development characters and economic characters of the silkworms are investigated. The results are shown in Table 4.

Table 4 Bombyx mori is fed with garlic water on its growth, development and economic characters

Remarks: Cocoon production refers to the cocoon production (kg) of 25,000 silkworms.

As can be seen from Table 4, the growth and development and economic traits of silkworms are affected to a certain extent after feeding the mulberry leaves treated with the mulberry leaf preservative of the present invention.

The fifth instar elapsed (day: hour) is in the order of treatment N < treatment J < treatment L < treatment M < treatment K < CK1 < CK2, and the fifth instar elapsed in the example is 12-30 h shorter than that in the control; The order from short to long is the same as the order of the five instars, the mulberry leaves treated with the mulberry leaf preservative of the present invention can promote the growth and development of silkworms, and the full-age elapsed time of the embodiment is also shortened by 12-30h compared with the control.

The order of the weight of mature silkworms from heavier to lighter was treatment L > treatment M > treatment N > treatment K > treatment J > CK1 > CK2; the order of whole cocoon weight and cocoon layer weight from heavier to lighter was consistent with the weight order of mature silkworms. The mulberry leaves treated with the invented mulberry leaf preservative can promote the growth and development of silkworms.

The order of cocoon formation rate from high to low is treatment L > treatment M > treatment N > treatment K > treatment J > CK1 > CK2; the order of dead cage rate from low to high is treatment L < treatment M < treatment K < treatment J <treatment N < CK1 < CK2; the order of healthy pupae rate of fourth instar silkworms from high to low was treatment L > treatment M > treatment K > treatment J > treatment N > CK1 > CK2. The health of silkworms is improved by eating the mulberry leaves treated with the mulberry leaf preservative of the present invention.

The order of cocoon production of 25000 silkworms from high to low was L>treatment M>treatment N>treatment K>treatment J>CK1>CK2. The cocoon production of the silkworms of the mulberry leaves after the mulberry leaf preservative treatment in Example 3 was the highest, and the cocoon production of the mulberry seed was 44.25kg, which was 8.88% higher than that of CK1 (41.56kg) and 10.07% higher than that of CK2 (40.2kg). Highly significant differences were achieved with treatments K, M, N, J and control.

To sum up, eating the mulberry leaves treated with the mulberry leaf preservative of the present invention can promote the growth and development of silkworms, shorten the whole age by 12 to 30 hours, and can increase the amount of cocoons. After mulberry leaves were fed to silkworms, the silkworms had the shortest growth and development time, high cocooning rate, low dead cage rate, and the cocoon production of the mulberry seeds was high, which was 44.25kg, which was 8.88% higher than that of CK1 (41.56kg) and CK2 (40.2kg). ) increased production by 10.07%. The differences between the treatments were extremely significant.

Application example 3

From May to June 2020, the feeding test of the mulberry leaf preservative of the present invention was carried out at the Fukang Sericulture Farmers Professional Cooperative Base in Fenggang County, Zunyi City, Guizhou Province. Three treatment areas were set up, and each treatment was set up with three replicate areas. In each replicate area, one silkworm seed was co-bred to the third instar and the fourth instar for the silkworm experiment, with a total of 9 replicate areas. The same room rearing, other technical operations are consistent. The mulberry leaf variety tested was Nongsang No. 14, and the silkworm variety was Huakang No. 2. Treatment O: The mulberry leaves that were treated with the mulberry leaf preservative of Example 3 and stored and preserved for 3-7 days were fed. CK1: The mulberry leaves collected on the previous day or on the same day were fed with mulberry leaves treated with water; CK2: The mulberry leaves were fed routinely on the previous day or on the same day, and the growth, development and economic characters of the silkworms were investigated. The results are shown in Table 5.

Table 5 The results of the investigation of the feeding test

The order of the five instars (day: hour) from short to long is the treatment O<CK1<CK2, and the order of the whole age from short to long is the same as the five instars. The leaves can promote the growth and development of silkworms, and the whole age is shortened by 18 to 24 hours.

The order of the dead cage rate from low to high is treatment O<CK1<CK2, indicating that the mulberry leaf preservative of the present invention has the effect of disinfection and sterilization, and enhances the disease resistance of silkworms.

The order of total cocoon amount from high to low is treatment O>CK1>CK2, and the order of cocoon layer amount and cocoon layer rate from high to low is consistent with the order of total cocoon amount. The storage of mulberry leaves can improve the quality of silkworm cocoons.

The order of cocoon production of 25000 silkworms from high to low was O>CK1>CK2. The difference between treatment O and treatment of CK1 and CK2 was extremely significant. Consuming the mulberry leaves treated with the mulberry leaf preservative in Example 3 of the present invention can increase the amount of cocoons produced. The amount of cocoons produced by the mulberry seeds is 49.69kg, which is 15.42% higher than that of CK2 (43.05kg) and 15.42% higher than that of CK1 (46.13kg). 10.10%.

Application example 4

From June to July 2020, the feeding experiment of the present invention was carried out in the laboratory of the Guizhou Sericulture Research Institute. Four treatment areas were set up, and each treatment was set up with three replicate areas. Each replicate area was co-bred with 1 silkworm seed until the third instar and the fourth instar. There were 12 replicate areas in total. The same room rearing, other technical operations are consistent. The mulberry leaf variety tested was Nongsang No. 14, and the silkworm variety was Huakang No. 2. Treatment P: Trial feeding of mulberry leaves treated with the mulberry leaf preservative of Example 2; Treatment Q: Trial feeding of mulberry leaves treated with the mulberry leaf preservative of Example 3; Treatment R: Using the EM activity of Comparative Example 4 Trial feeding of mulberry leaves treated with bacteria; CK1: Trial feeding of mulberry leaves treated with water. The growth and development characters and economic characters of the silkworms were investigated, and the results are shown in Tables 6 and 7.

Table 6 Feeding test investigation

Table 7 Investigation on the number of eggs laid by a single moth in the feeding experiment

The order of the fifth instar elapsed (day: hour) from short to long is treatment Q < treatment P < treatment R < CK1. The order of the whole age from short to long is the same as that of the fifth age. The mulberry leaves treated with the mulberry leaf preservative of the present invention can make silkworms grow and develop fast, and the whole age is shortened by 12-18 hours.

The order of dead cage rate from low to high is treatment Q < treatment P < treatment R < CK1, indicating that the mulberry leaf preservative of the present invention has the effect of disinfection and sterilization, and enhances the disease resistance of silkworms.

The order of total cocoon amount from high to low is treatment Q>treatment P>treatment R>CK1, and the order of cocoon layer amount and cocoon layer rate from high to low is consistent with the order of total cocoon amount. The mulberry leaves of the leaf preservative can improve the quality of silkworm cocoons.

The order of cocoon production of 25000 silkworms from high to low was treatment Q > treatment P > treatment R > CK1. Feeding the mulberry leaves treated with the mulberry leaf preservative of Example 3 of the present invention can increase the amount of cocoons produced, and the result of treatment Q is the best, and the amount of seed cocoons is 37.82kg, which is 2.35% higher than that of treatment R (36.95kg). Compared with CK1 (35.79kg), the yield was increased by 5.67%. Secondly, the amount of cocoons in P treatment was 37.29kg, which was 0.92% higher than that of P (36.95kg) and 4.91% higher than that of CK1 (35.79kg).

The order of the total number of eggs (grains) of a single moth from more to less was treatment Q>treatment P>treatment R>CK1. The order of the hatching rate on 3 days from high to low was consistent with the order of the total egg number of a single moth. The order of dead egg rate from low to high is Q<treatment P<treatment R<CK1. It is illustrated that the mulberry leaf of the mulberry leaf preservative in Example 3 of the present invention can improve the egg laying amount and the practical hatching rate of the single moth of the original silkworm.

It can be known from the results of the examples and application examples that the mulberry leaf preservative of the present invention has a good fresh-keeping effect, and after storage for 7 days, the water loss rate of mulberry leaves is below 10%; Leaves can promote the growth and development of silkworms. After the fifth instar is shortened by 12 to 30 hours, and the full instar is shortened by 6 to 30 hours, the cocoon formation rate is increased by 0.39% to 1.38%, and the dead cage rate is reduced by 0.11% to 0.48%. The rate of cocoon layer and cocoon layer increased by 2.02%-10.23%, 1.90%-15.22%, 0.81%-4.48%, respectively, the cocoon production of Zhang seed was increased by 4.91%-15.42%;

Although the present invention has been disclosed above with preferred embodiments, it is not intended to limit the present invention. Anyone who is familiar with this technology can make various changes and modifications without departing from the spirit and scope of the present invention. Therefore, The protection scope of the present invention should be defined by the claims.

Claims (10)

1. a mulberry leaf preservative, is characterized in that, active ingredient is garlic, and the mass percentage composition of garlic in described mulberry leaf preservative is 5%～10%. 2. mulberry leaf preservative according to claim 1, is characterized in that, the active ingredient of described mulberry leaf preservative also comprises white granulated sugar, and the mass percentage of white granulated sugar in described mulberry leaf preservative is 5%～ 10%. 3. The mulberry leaf preservative according to claim 2, wherein the mass percentage of garlic in the mulberry leaf preservative is 5%, and the mass percentage of the white granulated sugar is 5%. 4. The preparation method of the mulberry leaf preservative according to any one of claims 1 to 3, comprising the steps of: pulverizing the garlic raw material and then mixing it into water to obtain the mulberry leaf preservative. 5. The preparation method according to claim 4, wherein when the mulberry leaf preservative comprises white granulated sugar, the white granulated sugar and the crushed garlic are mixed into water to obtain the mulberry leaf preservative. 6 . The preparation method according to claim 4 , wherein the mixing time is 5-10 min; after the mixing, it also includes clarifying the mixed solution for 1-2 h to obtain the mulberry leaf preservative. 7 . . 7. the using method of the described mulberry leaf preservative of any one of claim 1～3, comprises the following steps, described mulberry leaf preservative is sprayed on the mulberry leaf, the mulberry leaf after spraying the preservative is stored, during storage Turn mulberry leaves every day. 8 . The method according to claim 7 , wherein the dosage of the mulberry leaf preservative per kilogram of mulberry leaves is 0.15 to 0.25 kg; the humidity of the storage is 75% to 90%; the The storage temperature is 20-28 ℃. 9 . The method according to claim 7 , wherein the mulberry leaves after spraying the preservative are covered with a perforated plastic film, and the diameter of the hole is 1-2 mm; The density is 30 to 50 pieces/m ² . 10. A method for increasing the production of silkworm cocoons, comprising the steps of: feeding the mulberry leaves treated with the preservative according to any one of claims 1 to 3.