CN112088880B - Application of apigenin in preparation of preparations for promoting plant growth and improving plant salt tolerance - Google Patents
Application of apigenin in preparation of preparations for promoting plant growth and improving plant salt tolerance Download PDFInfo
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- CN112088880B CN112088880B CN202010854231.4A CN202010854231A CN112088880B CN 112088880 B CN112088880 B CN 112088880B CN 202010854231 A CN202010854231 A CN 202010854231A CN 112088880 B CN112088880 B CN 112088880B
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- XADJWCRESPGUTB-UHFFFAOYSA-N apigenin Natural products C1=CC(O)=CC=C1C1=CC(=O)C2=CC(O)=C(O)C=C2O1 XADJWCRESPGUTB-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 235000008714 apigenin Nutrition 0.000 title claims abstract description 51
- KZNIFHPLKGYRTM-UHFFFAOYSA-N apigenin Chemical compound C1=CC(O)=CC=C1C1=CC(=O)C2=C(O)C=C(O)C=C2O1 KZNIFHPLKGYRTM-UHFFFAOYSA-N 0.000 title claims abstract description 51
- 229940117893 apigenin Drugs 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 230000001737 promoting effect Effects 0.000 title claims abstract description 21
- 230000015784 hyperosmotic salinity response Effects 0.000 title claims abstract description 19
- 230000008635 plant growth Effects 0.000 title claims abstract description 13
- 230000007226 seed germination Effects 0.000 claims abstract description 7
- 230000004083 survival effect Effects 0.000 claims abstract description 5
- 241000196324 Embryophyta Species 0.000 claims description 38
- 241000208125 Nicotiana Species 0.000 claims description 24
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 24
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000007864 aqueous solution Substances 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 6
- 239000008223 sterile water Substances 0.000 claims description 6
- 150000003839 salts Chemical class 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 4
- 239000002207 metabolite Substances 0.000 abstract description 3
- 238000005728 strengthening Methods 0.000 abstract description 2
- 230000035882 stress Effects 0.000 abstract 4
- 230000007123 defense Effects 0.000 abstract 1
- 230000036542 oxidative stress Effects 0.000 abstract 1
- LJRGBERXYNQPJI-UHFFFAOYSA-M sodium;3-nitrobenzenesulfonate Chemical compound [Na+].[O-][N+](=O)C1=CC=CC(S([O-])(=O)=O)=C1 LJRGBERXYNQPJI-UHFFFAOYSA-M 0.000 abstract 1
- 230000035784 germination Effects 0.000 description 33
- 244000062793 Sorghum vulgare Species 0.000 description 22
- 241000209140 Triticum Species 0.000 description 22
- 235000021307 Triticum Nutrition 0.000 description 22
- 235000019713 millet Nutrition 0.000 description 22
- 230000012010 growth Effects 0.000 description 16
- 239000000243 solution Substances 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 7
- 239000011780 sodium chloride Substances 0.000 description 4
- 239000011521 glass Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 241000628997 Flos Species 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000195975 Selaginellaceae Species 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 241001534930 Thymelaeaceae Species 0.000 description 1
- 241001073567 Verbenaceae Species 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002936 tranquilizing effect Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Of Plants (AREA)
Abstract
本发明公开了芹菜素在制备促进植物生长、提高植物耐盐性的制剂中的应用。芹菜素作为一种植物代谢物，能够在植物抵御氧化胁迫过程中发挥功能。本发明所述芹菜素施用于受到盐胁迫的植物种子，能够显著提高种子在盐胁迫下的萌发率，和萌发种子速度；能够显著提高盐胁迫下植物幼苗的株高、根长、鲜重或存活率，提高植物抵御盐胁迫的能力；正常条件下施加芹菜素，也能够促进植物生长，增加芽长、根长和鲜重。因此，芹菜素在保苗、壮苗、提高盐胁迫方面具有重要作用。本发明的应用方法使用简单、效果显著。
The invention discloses the application of apigenin in preparing a preparation for promoting plant growth and improving plant salt tolerance. As a plant metabolite, apigenin can play a role in plant defense against oxidative stress. The apigenin of the present invention is applied to the plant seeds under salt stress, can significantly improve the seed germination rate and seed germination speed under salt stress; can significantly improve the plant height, root length, fresh weight or Survival rate, improve the ability of plants to resist salt stress; application of apigenin under normal conditions can also promote plant growth, increase shoot length, root length and fresh weight. Therefore, apigenin plays an important role in protecting seedlings, strengthening seedlings and improving salt stress. The application method of the present invention is simple to use and has remarkable effects.
Description
Technical Field
The invention belongs to the field of biology, and particularly relates to application of apigenin in preparation of a preparation for promoting plant growth and improving plant salt tolerance.
Background
The preparation method of the apigenin comprises the following steps of,
belongs to flavone, and mainly exists in Thymelaeaceae, Verbenaceae and Selaginellaceae plants, such as flos Genkwa and herba Selaginellae with high content. Has carcinogenic activity of carcinogenic substance inhibiting effect, and can be used as antiviral drug for treating HIV and other virus infection; can be used for treating various inflammations; can resist oxidation; has effects of tranquilizing mind; and (4) the efficacy of reducing blood pressure. As a metabolite endogenous to the plant, against the plantIn the process of resisting salt stress, the content of apigenin is greatly changed, which indicates that the apigenin possibly participates in the process of resisting salt stress of plants.
Soil salinization seriously affects the growth and quality of crops, and becomes one of the main environmental factors limiting the yield of the current crops. High salt ultimately leads to a reduction in economic crop yield by affecting seed germination, seedling growth and vigor, flowering and fruiting of plants, etc. (Sairam & Tyagi, 2004). Therefore, the efficient utilization of saline soil and the improvement of the salt resistance of crops have become global and widely concerned problems.
Disclosure of Invention
In view of the situations of the prior art, the invention aims to provide the application of apigenin in preparing a preparation for promoting plant growth and improving plant salt tolerance.
In order to realize the purpose of the invention, the invention is realized by adopting the following technical scheme:
the invention provides application of apigenin in preparation of a preparation for promoting plant growth.
The invention also provides application of the apigenin in preparation of a preparation for improving the salt tolerance of plants.
Further, the specific application method is as follows: applying an aqueous solution of the formulation to plant seeds or seedlings.
Further, the application comprises the following specific steps:
sterilizing the surface of the plant with ethanol, washing with sterile water, and soaking the plant seed in the water solution of the preparation; or soaking the roots of young plants in the aqueous solution of the preparation.
Furthermore, the concentration of the apigenin in the aqueous solution of the preparation is 0.4-0.7 mu mol/L.
Further, the plant seeds are soaked for 12-16 h; the soaking time of the roots of the plant seedlings is 4 to 7 days.
Further, the plant seeds or seedlings are seeds or seedlings of wheat, millet and tobacco.
Furthermore, the apigenin can improve the germination rate of seeds, the germination potential of the seeds, the bud length of seedlings, the root length of seedlings, the fresh weight of the seedlings and the survival rate of the seedlings.
Compared with the prior art, the invention has the advantages and positive effects that: the invention provides application of apigenin in promoting plant growth and improving plant salt tolerance, and the apigenin serving as a plant metabolite can play a role in plants. The growth can be promoted by applying apigenin aqueous solution under normal conditions; the application of the fertilizer under the condition of salt stress can improve the germination rate and the germination vigor of seeds and reduce salt damage; can also improve the height, the root length, the fresh weight and the survival rate of the plants, and can achieve the purposes of protecting and strengthening the seedlings and improving the salt stress resistance in production. The invention provides the application of apigenin in promoting plant growth and improving plant salt tolerance, and the application is simple and has obvious effect.
Drawings
Fig. 1 is a growth promoting effect of apigenin on wheat, millet and tobacco seeds, wherein A: growth promoting effect of apigenin on wheat seeds, B: growth promoting effect of apigenin on millet seeds, C: the growth promotion effect of the apigenin on tobacco seeds is realized;
FIG. 2 shows the growth promoting effect of apigenin on wheat seedlings;
FIG. 3 shows the growth promoting effect of apigenin on millet seedlings;
FIG. 4 shows the growth promoting effect of apigenin on tobacco seedlings;
FIG. 5 shows the salt tolerance promoting effect of apigenin on wheat seeds, millet seeds and tobacco seeds; wherein, A: promoting effect of apigenin on salt tolerance of wheat seeds, wherein B: promoting effect of apigenin on salt tolerance of millet seeds, wherein the ratio of apigenin to salt is as follows: promoting the salt tolerance of tobacco seeds by using apigenin;
FIG. 6 shows the effect of apigenin on the promotion of salt tolerance of wheat seedlings;
FIG. 7 shows the effect of apigenin on the promotion of salt tolerance of millet seedlings;
FIG. 8 shows the effect of apigenin on the promotion of salt tolerance of tobacco seedlings.
Detailed Description
The technical solution of the present invention will be described in further detail with reference to the accompanying drawings and the detailed description.
Example 1 apigenin can promote plant growth: seed germination and seedling growth
1. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. And (3) soaking the seeds of the control group and the experimental group in corresponding treatment solutions for 12 hours respectively for pretreatment. Three replicates of each treatment, each 50 seeds in parallel.
The soaked seeds were spread on a 9 cm glass petri dish previously filled with sterilized filter paper and cultured at 25 ℃.5 ml of the corresponding treatment solution was added to each dish. And counting germination data every day, and counting bud length, root length and fresh weight. The germination rate of the seeds is the germination rate of wheat on the 2 nd day, the germination rate of the millet on the 3 rd day and the germination rate of the tobacco on the 5 th day; the germination vigor of wheat is the germination vigor of 4 days, the germination vigor of millet is the germination vigor of 5 days, and the germination vigor of tobacco is the germination vigor of 7 days; seedling height, root length and fresh weight, wheat is the plant height, root length and fresh weight at the 4 th day after treatment, millet is the plant height, root length and fresh weight at the 5 th day after treatment, and tobacco is the plant height, root length and fresh weight at the 7 th day after treatment.
TABLE 1 growth promoting effect of apigenin on wheat, millet and tobacco seeds
Note:＊＊＊P＜0.001，＊＊P＜0.01，＊P＜0.05。
in the table, CK is distilled water and CK + 0.5. mu. MAP is an aqueous solution containing 0.5. mu.M apigenin.
Germination rate is 100% of the number of germinated seeds/total number of seeds.
Germination vigor is 100% of the number of normally germinated seeds/total number of test seeds within the number of days of germination.
As can be seen from table 1 and A, B of fig. 1, exogenous application of apigenin significantly promoted plant seed germination and post-germination growth compared to controls.
2. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. After wheat sprouts for 2 days, millet sprouts for 3 days and tobacco sprouts for 5 days, selecting seedlings with consistent growth vigor, and carrying out corresponding solution treatment. And (5) observing the phenotype. And counting the bud length, the root length and the fresh weight. As can be seen from fig. 2, 3 and 4, exogenous application of apigenin under normal conditions significantly promoted the growth of plants compared to controls.
Example 2 apigenin can improve salt tolerance of plant seeds
1. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. The seeds of the control group and the experimental group are respectively soaked in the treatment solution for 12 hours for pretreatment. Three replicates of each treatment, 50 seeds per replicate.
The soaked seeds were spread on a 9 cm glass petri dish previously filled with sterilized filter paper and cultured at 25 ℃.5 ml of the corresponding treatment solution was added to each dish. Germination data were counted daily. The germination rate of the seeds is the germination rate of wheat on the 2 nd day, the germination rate of the millet on the 3 rd day and the germination rate of the tobacco on the 5 th day; the germination vigor of wheat is the germination vigor of 4 days, the germination vigor of millet is the germination vigor of 5 days, and the germination vigor of tobacco is the germination vigor of 7 days; seedling height, root length and fresh weight, wheat is the plant height, root length and fresh weight at the 4 th day after treatment, millet is the plant height, root length and fresh weight at the 5 th day after treatment, and tobacco is the plant height, root length and fresh weight at the 7 th day after treatment.
TABLE 2 promoting effect of apigenin on salt tolerance of wheat, millet and tobacco seeds
Note:＊＊＊P＜0.001，＊＊P＜0.01，＊P＜0.05
the concentration of NaCl solution in the table was 150mmol/L, and NaCl + 0.5. mu.M AP was an aqueous solution containing 150mmol/L NaCl solution and 0.5. mu. mol/L apigenin. Germination rate is 100% of the number of germinated seeds/total number of seeds. Germination vigor is 100% of the number of normally germinated seeds/total number of test seeds within the number of days of germination.
It is clear from table 2 and A, B, C of fig. 5 that exogenous application of apigenin significantly promoted germination and growth of wheat, millet and tobacco seeds under salt stress, as compared to the control.
Example 3 apigenin can improve salt tolerance of young plants
1. And selecting full and healthy wheat seeds, millet seeds and tobacco seeds. The seed surface was first sterilized with 70% ethanol for 20 seconds and then washed 5-6 times with sterile water. After wheat sprouts for 2 days, millet sprouts for 3 days and tobacco sprouts for 5 days, selecting seedlings with consistent growth vigor, and carrying out corresponding solution treatment. And (5) observing the phenotype. And counting the bud length, the root length, the fresh weight and the survival rate.
As can be seen from fig. 6, 7 and 8, exogenous application of apigenin under salt stress conditions significantly promoted the growth of plants, compared to the control.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (5)
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106211857A (en) * | 2011-09-08 | 2016-12-14 | 诺维信生物农业公司 | Method for treating seeds and compositions |
| CN106386871A (en) * | 2016-08-31 | 2017-02-15 | 山东胜伟园林科技有限公司 | Rooting agent containing celery extract and preparation method thereof |
| CN108174872A (en) * | 2018-01-11 | 2018-06-19 | 和县农丰蔬菜种子专业合作社 | A kind of vegetable seeds sterilization and activation inorganic agent and its preparation and application |
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- 2020-08-21 CN CN202010854231.4A patent/CN112088880B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106211857A (en) * | 2011-09-08 | 2016-12-14 | 诺维信生物农业公司 | Method for treating seeds and compositions |
| CN106386871A (en) * | 2016-08-31 | 2017-02-15 | 山东胜伟园林科技有限公司 | Rooting agent containing celery extract and preparation method thereof |
| CN108174872A (en) * | 2018-01-11 | 2018-06-19 | 和县农丰蔬菜种子专业合作社 | A kind of vegetable seeds sterilization and activation inorganic agent and its preparation and application |
Non-Patent Citations (2)
| Title |
|---|
| Ahmad Mohammad M. Mkeawy etl.Apigenin pretreatment enhances growth and salinity tolerance of rice Seedlings.《Plant physiology and Biochemistry》.2018, * |
| Apigenin pretreatment enhances growth and salinity tolerance of rice Seedlings;Ahmad Mohammad M. Mkeawy etl;《Plant physiology and Biochemistry》;20180630;摘要、2材料和方法、3结果部分、图表1A-C * |
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