CN112075424A - Solution formula capable of promoting adherence of onchocercaria and infecting dodder leaves - Google Patents
Solution formula capable of promoting adherence of onchocercaria and infecting dodder leaves Download PDFInfo
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- CN112075424A CN112075424A CN202011017110.0A CN202011017110A CN112075424A CN 112075424 A CN112075424 A CN 112075424A CN 202011017110 A CN202011017110 A CN 202011017110A CN 112075424 A CN112075424 A CN 112075424A
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- dodder
- leaves
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- adherence
- promoting
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- 230000001737 promoting effect Effects 0.000 title claims abstract description 11
- 240000007371 Cuscuta campestris Species 0.000 title abstract description 23
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 12
- 239000011734 sodium Substances 0.000 claims abstract description 12
- 229940077388 benzenesulfonate Drugs 0.000 claims abstract description 10
- -1 sodium alkyl benzene Chemical class 0.000 claims abstract description 10
- 208000015181 infectious disease Diseases 0.000 claims abstract description 9
- 239000003085 diluting agent Substances 0.000 claims abstract description 7
- 241000222199 Colletotrichum Species 0.000 claims abstract description 6
- 229930006000 Sucrose Natural products 0.000 claims abstract description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 6
- 229920004890 Triton X-100 Polymers 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 8
- 241001529387 Colletotrichum gloeosporioides Species 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 238000007865 diluting Methods 0.000 claims description 2
- 238000003672 processing method Methods 0.000 claims description 2
- 241000110637 Cuscuta chinensis Species 0.000 claims 3
- 238000009472 formulation Methods 0.000 claims 2
- 230000000694 effects Effects 0.000 abstract description 5
- 229960004793 sucrose Drugs 0.000 abstract description 4
- 241001248590 Isaria Species 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 17
- 239000000243 solution Substances 0.000 description 15
- 230000001580 bacterial effect Effects 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 241000228143 Penicillium Species 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 238000005507 spraying Methods 0.000 description 5
- 239000012895 dilution Substances 0.000 description 4
- 238000010790 dilution Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 210000000582 semen Anatomy 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 3
- 229920000053 polysorbate 80 Polymers 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000008121 dextrose Substances 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- ZPIRTVJRHUMMOI-UHFFFAOYSA-N octoxybenzene Chemical compound CCCCCCCCOC1=CC=CC=C1 ZPIRTVJRHUMMOI-UHFFFAOYSA-N 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/24—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients to enhance the sticking of the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Agronomy & Crop Science (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a formula of a solution capable of promoting the adherence of the colletotrichum tracinum and infecting the leaves of the dodder, wherein the solution comprises a diluent of the colletotrichum tracinum, and is characterized in that: also comprises sodium alkyl benzene sulfonate, TritonX100 and cane sugar. The invention well improves the effects of adherence of the isaria and the infection of the dodder and improves the removal effect of the dodder.
Description
Technical Field
The invention relates to the technical field of a solution of the strain of the Penicillium, in particular to a formula of a solution capable of promoting the adhesion of the strain of the Penicillium and the infection of the leaves of the dodder.
Background
After farmers take the bacterial liquid, hypha and spores are easy to slip off from the seed leaves of the dodder because the bacterial liquid is not sprayed well, and the hypha and the spores are less adhered to the surfaces of the leaves. In addition, hypha and spores adhered to the surface of the dodder leaf need nutrient substances for continuous propagation. In order to overcome the above problems, the present inventors developed an amellaria discoidea solution for promoting adhesion and infecting dodder, thereby achieving the effect of killing dodder.
Disclosure of Invention
The invention aims to provide a solution formula capable of promoting the adherence of the discothrix bacteria and the infection of the dodder leaves, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the solution can promote the adherence of the colletotrichum tragophyllum and infect the prescription of the dodder leaf, and the solution comprises diluent of the colletotrichum tragophyllum, sodium alkyl benzene sulfonate, Triton X100 and sucrose.
Preferably, the mass percent of the sodium alkyl benzene sulfonate in the solution is 0.05-0.1%, the mass percent of the Triton X100 in the solution is 0.01-0.05%, and the mass percent of the sucrose in the solution is 0.5-1%.
Preferably, the processing method of the colletotrichum gloeosporioides diluent comprises the steps of smashing hyphae and spores of the colletotrichum gloeosporioides, diluting according to a certain proportion, and then adding different components into the diluted colletotrichum gloeosporioides diluent.
Compared with the prior art, the invention has the beneficial effects that:
the invention well improves the effects of adherence of the isaria and the infection of the dodder and improves the removal effect of the dodder.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The invention provides a technical scheme that: the preparation method comprises adding 500ml of bacteria solution into 2L water, stirringSmashing hypha with a crusher, pouring into 198L water after smashing fully, adding 200g sodium alkyl benzene sulfonate, and stirring uniformly with a crusher for dissolving. Then added to the watering can. Then spraying the mixture on the leaves of the dodder for 1 mu of land. And randomly collecting 10 semen Cuscutae leaves on the same day, and counting viable count of the Penicillium discodermatum in each square centimeter of leaf surface by using a plate counting method. As a result, it was found that the number of viable bacteria per square centimeter of leaf surface was (2.48. + -. 0.23). 10 when 0.1% sodium alkylbenzenesulfonate was added5The number of viable bacteria on the surface of the leaf blade is obviously higher than that of the viable bacteria on the surface of the leaf blade without adding the viable bacteria per square centimeter.
Example 2
The invention provides a technical scheme that: the formula of the solution capable of promoting the adherence of the discothrix bacteria and the infection of the dodder leaves comprises the steps of pouring 500ml of bacterial liquid into 2L of water, smashing hyphae by a blender, pouring the smashed hyphae into 198L of water after full smashing, adding 100ml of Triton X100 (polyethylene glycol octyl phenyl ether), and stirring uniformly and dissolving by the blender. Then added to the watering can. Then spraying the mixture on the leaves of the dodder for 1 mu of land. And randomly collecting 10 semen Cuscutae leaves on the same day, and counting viable count of the Penicillium discodermatum in each square centimeter of leaf surface by using a plate counting method. As a result, it was found that the number of viable bacteria per square centimeter of leaf surface was (1.23. + -. 0.13). times.10 when 0.05% Triton X100 was added5The number of viable bacteria on the surface of the leaf blade is obviously higher than that of the viable bacteria on the surface of the leaf blade without adding the viable bacteria per square centimeter.
Example 3
The invention provides a technical scheme that: the solution formula can promote the adherence of the discodermospora and the infection of the dodder leaves, 500ml of bacterial liquid is poured into 2L of water, hypha is smashed by a blender, the smashed hypha is poured into 198L of water after being fully smashed, 200g of sodium alkyl benzene sulfonate and 100ml of Triton X100 are added, and the mixture is stirred uniformly and dissolved by the blender. Then added to the watering can. Then spraying the mixture on the leaves of the dodder for 1 mu of land. And randomly collecting 10 semen Cuscutae leaves on the same day, and counting viable count of the Penicillium discodermatum in each square centimeter of leaf surface by using a plate counting method. As a result, it was found that the number of viable bacteria per square centimeter of leaf surface was (1.63. + -. 0.09). 10 after adding the two components6Obviously higher than the addition of 0.1 percent of sodium alkyl benzene sulfonate per square centimeter of the surface viable bacteria of the leaves (example 1) and the addition of 0.05 percent of TritonX100 viable count per square centimeter of leaf surface and viable count per square centimeter of leaf surface without any additive.
Example 4
The invention provides a technical scheme that: the formula of the solution capable of promoting the adherence of the discodermospora and the infection of the dodder leaves comprises the steps of pouring 500ml of bacterial liquid into 2L of water, smashing hypha by a blender, pouring into 198L of water after smashing fully, adding 200g of sodium alkyl benzene sulfonate, 100ml of Triton X100 and 200g of cane sugar, and stirring uniformly and dissolving by the blender. Then added to the watering can. Then spraying the mixture on the leaves of the dodder for 1 mu of land. And randomly collecting 10 semen Cuscutae leaves on the same day, and counting viable count of the Penicillium discodermatum in each square centimeter of leaf surface by using a plate counting method. As a result, it was found that the number of viable bacteria per square centimeter of leaf surface was (1.73. + -. 0.012). times 10 after the addition of the three components6And the number of viable bacteria on the surface of the leaf per square centimeter is not different from that of the leaf surface after sodium alkyl benzene sulfonate and Triton X100 are added.
And observing the number of the yellow spots on the surface of the dodder leaves after 1 week, and finding that the number of the yellow spots is remarkably higher than that of the yellow spots on the surface of the dodder leaves after 1 week after spraying after only adding sodium alkyl benzene sulfonate and Triton X100.
The detection method of the viable count of the leaves in unit area comprises the following steps:
plates were counted on a Sabourne dextrose agar plate (chloramphenicol). Collected leaves were cut into an area of 1 square centimeter (side length: 1cm), and were put into 100ml Erlenmeyer flasks (previously sterilized and 2mm glass beads added) containing 10ml of sterile PBS (containing 0.5% Tween 80, pH7.0-7.4), followed by vigorous shaking to prepare a bacterial suspension. Then 1ml of the bacterial suspension is added into a test tube filled with 9ml of sterile PBS (containing 0.5% Tween 80, pH7.0-7.4), and the mixture is mixed evenly. Then, 1ml of the previously prepared bacterial suspension was added to a test tube containing 9ml of sterile PBS (containing 0.5% Tween 80, pH7.0-7.4), and mixed well. By analogy, 10-fold dilution was performed. 200ul of bacterial suspension with the dilution times of 109, 1010, 1011 and 1012 is taken and coated on a 90mm Sabouraud's dextrose agar culture dish, the culture is carried out for 3 to 5 days at 28 ℃, the culture dish with 25 to 100 bacterial colonies is taken as an effective culture dish, and the bacterial colonies on the culture dish are counted. And calculating according to the following formula to obtain the concentration of viable bacteria in the original bacterial liquid. 3 dishes were made for each dilution, and the mean value was taken and the standard error was calculated.
Viable bacteria per square centimeter of leaf surface (number of colonies on the effective culture dish) 50 dilution times.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. The formula of the solution capable of promoting the adherence of the colletotrichum tragophyllum and infecting the dodder leaves comprises a diluent of the colletotrichum tragophyllum, and is characterized in that: also included are sodium alkylbenzenesulfonate, Triton X100 and sucrose.
2. The solution formulation for promoting the adherence of a. disciosum and the infection of a cuscuta chinensis leaf according to claim 1, wherein: the mass percent of the sodium alkyl benzene sulfonate in the solution is 0.05-0.1%, the mass percent of the Triton X100 in the solution is 0.01-0.05%, and the mass percent of the sucrose in the solution is 0.5-1%.
3. The solution formulation for promoting the adherence of a. disciosum and the infection of a cuscuta chinensis leaf according to claim 1, wherein: the processing method of the colletotrichum gloeosporioides diluent comprises the steps of smashing hypha and spores of the colletotrichum gloeosporioides, diluting according to a certain proportion, and then adding different components into the diluted colletotrichum gloeosporioides diluent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011017110.0A CN112075424A (en) | 2020-09-24 | 2020-09-24 | Solution formula capable of promoting adherence of onchocercaria and infecting dodder leaves |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202011017110.0A CN112075424A (en) | 2020-09-24 | 2020-09-24 | Solution formula capable of promoting adherence of onchocercaria and infecting dodder leaves |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN112075424A true CN112075424A (en) | 2020-12-15 |
Family
ID=73739838
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202011017110.0A Pending CN112075424A (en) | 2020-09-24 | 2020-09-24 | Solution formula capable of promoting adherence of onchocercaria and infecting dodder leaves |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN112075424A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1211898A (en) * | 1996-12-25 | 1999-03-24 | 日本烟草产业株式会社 | Waterbourne microbial pesticides |
| CN104542609A (en) * | 2015-01-05 | 2015-04-29 | 乔建国 | Herbicide for preventing and removing semen cuscutae |
| CN105360168A (en) * | 2015-12-07 | 2016-03-02 | 井冈山大学 | Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus |
| CN107513501A (en) * | 2017-09-27 | 2017-12-26 | 上海瑞楚生物科技有限公司 | The long spore bacterium Spawn incubation of disk and technical process |
| CN112535173A (en) * | 2020-12-02 | 2021-03-23 | 武汉庆发禾盛农业发展有限公司 | Vegetable seed sterilization and activation treatment agent and preparation method thereof |
-
2020
- 2020-09-24 CN CN202011017110.0A patent/CN112075424A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1211898A (en) * | 1996-12-25 | 1999-03-24 | 日本烟草产业株式会社 | Waterbourne microbial pesticides |
| CN104542609A (en) * | 2015-01-05 | 2015-04-29 | 乔建国 | Herbicide for preventing and removing semen cuscutae |
| CN105360168A (en) * | 2015-12-07 | 2016-03-02 | 井冈山大学 | Method used for preventing alien weed Alternanthera philoxeroides with pathogenic fungus |
| CN107513501A (en) * | 2017-09-27 | 2017-12-26 | 上海瑞楚生物科技有限公司 | The long spore bacterium Spawn incubation of disk and technical process |
| CN112535173A (en) * | 2020-12-02 | 2021-03-23 | 武汉庆发禾盛农业发展有限公司 | Vegetable seed sterilization and activation treatment agent and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 张龙 等: "氯氰菊酯微乳剂在农作物叶面的润湿展布性研究", 《江苏农业科学》 * |
| 葛秀涛 等: "《物理化学》", 30 August 2014, 中国科学技术大学出版社 * |
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Application publication date: 20201215 |
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