CN112063519B - Photosynthetic bacteria continuous culture device and control method thereof - Google Patents

Abstract

The invention belongs to the fields of biotechnology and environmental protection, and relates to a continuous cultivation device for photosynthetic bacteria and a control method thereof. A feeding port and an exhaust pipe are installed on the top; a transparent sleeve with a rotating motor is installed in the middle of the culture tank. Inside the transparent sleeve is a heat dissipation pipe fixed on the culture tank and screwed with LED light strips. Several brush rods distributed along the circumference of the transparent casing are fixedly installed, and several brushes are fixedly installed on each brush rod; a side wall ruler is fixedly installed on the outer wall of the culture tank, and a number of liquid fluids are installed on the sliding card of the side wall ruler. The position detector can solve the light shadow existing in the photobioreactor in the existing photosynthetic bacteria culture technology, which causes photosynthetic bacteria to adhere to the light source or light receiving surface of the reactor to form a photobiofilm, which limits the continuous automatic cultivation of photosynthetic bacteria and increases the production cost of photosynthetic bacteria. The problem.

Description

A kind of photosynthetic bacteria continuous culture device and control method thereof

technical field

The invention belongs to the fields of biotechnology and environmental protection, and relates to a continuous cultivation device for photosynthetic bacteria and a control method thereof, in particular to a continuous cultivation device for photosynthetic bacteria for biological enhancement of wastewater and a control method thereof.

Background technique

Photosynthetic bacteria are a diverse group in terms of morphology, physiology and phylogeny, which widely exist in water, soil and activated sludge in nature. In different environments, photosynthetic bacteria show a variety of metabolic modes, and can use small molecular organics, sulfides, etc. as electron donors to grow under anaerobic light, aerobic light or aerobic dark conditions, thereby purifying water quality. Photosynthetic bacteria have various functions such as nitrogen fixation, denitrification, carbon fixation, and sulfide oxidation. Therefore, photosynthetic bacteria have been widely used in aquaculture, bio-enhanced treatment and resource utilization of black and odorous water bodies and organic wastewater.

At present, the cultivation of photosynthetic bacteria mainly adopts the method of artificial cultivation, and it is difficult to realize automatic continuous cultivation. The main reason is that the photobioreactor has the problem of "light shadow": due to the phototaxis of photosynthetic bacteria, photosynthetic bacteria are easily concentrated and attached to the light source or light receiving surface of the reactor, forming a dense photobiofilm, which hinders the penetration of light. pass further. This makes people have to frequently clean the light source or light-receiving surface of the reactor, which limits the continuous automatic cultivation of photosynthetic bacteria and increases the production cost of photosynthetic bacteria.

SUMMARY OF THE INVENTION

In view of this, the present invention solves the problem that the light shadow existing in the photobioreactor in the existing photosynthetic bacteria culture technology causes photosynthetic bacteria to adhere to the light source or light-receiving surface of the reactor to form a photobiofilm, so as to limit the continuous automatic cultivation of photosynthetic bacteria and increase the photosynthetic effect. In view of the problem of bacterial production cost, a continuous culturing device for photosynthetic bacteria with simple structure and low production cost, which can be used for large-scale cultivation, and a control method thereof are provided.

In order to achieve the above purpose, the present invention provides a continuous cultivation device for photosynthetic bacteria, which includes a culture tank and a storage tank arranged on the outside of the culture tank. The feeding pipe connected to the feeding tank and the return pipe that facilitates the circulation of the bacterial liquid in the culture tank are connected with two branch pipes, one of which is connected to the storage tank through the feeding pump, and the other branch is connected to the storage tank through the feeding pump. The water pump is connected to tap water, a drain pump is installed on the discharge pipe, a circulation pump is connected to the return pipe, and a feeding port and an exhaust pipe are installed on the top of the culture tank;

A transparent sleeve with a rotating motor is installed in the middle of the culture tank. Inside the transparent sleeve is a heat dissipation pipe fixed on the culture tank and screwed with LED light strips. A liquid sealing box is fixed at the connection between the transparent sleeve and the culture tank. , a number of brush rods distributed along the circumference of the transparent sleeve are fixedly installed on the outside of the transparent sleeve, the brush rods are fixedly connected with the culture tank, and each brush rod is fixedly installed with a number of brushes evenly distributed along the radial direction of the transparent sleeve. , the free end of the brush is in contact with the outer wall of the transparent sleeve;

A side wall scale is fixedly installed on the outer wall of the culture tank, and several liquid level detectors are slidably connected to the side wall scale. The liquid level detector, feed pump, inlet pump, circulation pump and drainage pump are connected to the same programmable controller .

The beneficial effect of the basic scheme is that the liquid sealing box plays the role of sealing the connection between the transparent sleeve and the culture tank, preventing the culture liquid in the culture tank from entering the transparent sleeve and damaging the heat dissipation pipe and the LED light strip. The rotating motor drives the transparent sleeve to rotate, so that the outer wall of the transparent sleeve rotates continuously along the brush rod, scrapes the photosynthetic bacteria remaining on the outer wall of the transparent sleeve, and prevents the photosynthetic bacteria from gathering on the outer wall of the transparent sleeve. The circulating pump continuously circulates the bacterial liquid at the conical bottom of the culture tank. According to the detection data of different liquid level detectors, the opening and closing of the feed pump, the feed pump, the circulation pump and the drain pump can be controlled in real time through the programmable controller.

Further, a support frame for supporting a heat dissipation pipe and a transparent sleeve is embedded in the culture tank, an upper cover plate is installed on the top of the culture tank, the upper cover plate is sealed and fitted with the inner wall of the cylindrical culture tank, and the liquid sealing box is installed on the upper cover. On the cover plate, the feeding port and the exhaust pipe are installed on the upper cover plate, and a circular limit hole adapted to the transparent sleeve is opened in the middle of the support frame. Beneficial effects: the upper end of the transparent sleeve is fixed on the bearing of the rotating motor, the lower end penetrates through the support frame and can be rotated within the limit hole range, and the transparent sleeve is driven by the rotating motor to rotate.

Further, there are several U-shaped card slots on the side wall scale, each U-shaped card slot is threaded with positioning bolts, and each U-shaped card slot is installed with a data cable and an infrared emission window. The detector and the liquid level detector slide on the side wall ruler through the corresponding U-shaped slot, and the infrared emission window of the liquid level detector is closely attached to the outer wall of the culture tank. Beneficial effects: The cooperation of the liquid level detector and the side wall ruler facilitates the programmable controller to accurately measure the amount of bacterial liquid in the culture tank.

Further, the upper end of the brush rod is fixed on the upper cover plate of the culture tank, and the lower end is fixed on the support frame.

Further, a number of circular holes are opened at the part of the transparent sleeve extending out of the liquid sealing box and exposed to the outside of the culture tank. Beneficial effects: the round holes on the transparent sleeve are conducive to the discharge of hot air in the transparent sleeve.

Further, the number of liquid level detection counters is 4, indicating the warning liquid level, reaction liquid level, feed liquid level and seed liquid level from top to bottom, and the ratio of the volume corresponding to the seed liquid level to the effective volume of the culture tank. The ratio of the volume corresponding to the feed liquid level to the effective volume of the culture tank is 1:10 to 1:2.

Further, an overflow pipe is installed on the outer wall of the culture tank between the reaction liquid level and the warning liquid level. Beneficial effect: It is convenient to discharge the excess bacterial liquid in the culture tank to the external system.

Further, the bottom of the culture tank is a conical bottom. Beneficial effect: The culture tank with the conical bottom facilitates the discharge of bacterial liquid.

Further, the thickness of a single brush rod is not more than 0.5cm.

Further, the heat pipe is composed of aluminum-based or copper-based materials and is hollow inside. Beneficial effect: The hollow heat pipe is convenient for the heat dissipation in the heat pipe.

Further, the materials of the culture tank and the transparent sleeve are both plexiglass or tempered glass. Beneficial effects: The culture tank and transparent casing made of plexiglass or tempered glass are convenient for real-time observation of bacterial culture.

A control method of a photosynthetic bacteria continuous culture device, comprising the following steps:

A. After the photosynthetic bacteria continuous culture device is assembled, manually add the microbial seed liquid from the feeding port on the top of the culture tank to the liquid level of the seed liquid;

B. Turn on the intelligent control system, the feed pump is turned on under the control of the programmable controller, and the culture medium is pumped from the storage tank to the culture tank. When the feeding liquid level is reached, the feed pump is automatically turned off, and the feed pump automatically Turn on, when the reaction liquid level is reached, the inlet water pump is automatically turned off, and the circulation pump is turned on automatically. After the circulation pump is turned on, the LED light strip and the rotating motor in the lighting system are automatically turned on, and the microorganisms in the culture tank begin to be cultivated;

C. After the microorganism has been cultivated for the set time, the LED light strip and the circulating pump in the lighting system are automatically turned off, the drainage pump on the discharge pipe is turned on, and the cultured bacterial liquid is put into the external system. When the liquid level is reached, the drain pump on the discharge pipe is automatically turned off;

D. The feed pump is turned on, and steps B and C are repeated to carry out the next cycle of photosynthetic bacteria cultivation, so as to realize the intelligent control of continuous cultivation and continuous delivery of photosynthetic bacteria. During the continuous cultivation of photosynthetic bacteria, the upper part of the culture tank is set Warning liquid level, when the culture tank fails so that the liquid in the tank exceeds the warning liquid level, the drainage pump will automatically open to drain water, and the drainage pump will be turned off until the liquid level drops to the reaction liquid level.

The beneficial effects of the present invention are:

1. In the device for continuous cultivation of photosynthetic bacteria disclosed by the present invention, the transparent sleeve is connected to the rotating motor, and the rotating motor drives the transparent sleeve to rotate to realize that the brush rod is continuously scraped along the photosynthetic bacteria on the inner wall of the culture tank, and the surface of the light source is removed in real time. Biofilm, avoids light attenuation caused by biofilm formation in the photobioreactor, saves the cost of manual removal, and enables the photobioreactor to operate continuously and automatically.

2. The device for continuous cultivation of photosynthetic bacteria disclosed in the present invention adopts the rotation of the central transparent sleeve instead of the rotation of the outer brush to realize the removal of the biofilm on the surface of the light source, which is beneficial to ensure the sealing effect between the rotating motor and the culture tank. The lighting system has a self-cleaning function, which can remove the biofilm on the surface of the light source at any time. Combined with the time control and liquid level control system, the continuous cultivation and release of photosynthetic bacteria is realized. The device has a simple and reliable structure, simple operation and management, and saves labor and maintenance costs.

3. The continuous cultivation device for photosynthetic bacteria disclosed in the present invention intelligently controls the operation of the lighting system and the water inlet and outlet systems through the cultivation time and liquid level detector, thereby realizing the continuous cultivation and dosing of photosynthetic bacteria and greatly reducing the operation management. The difficulty increases the effect of bio-enhancement.

Other advantages, objects, and features of the present invention will be set forth in the description that follows, and will be apparent to those skilled in the art based on a study of the following, to the extent that is taught in the practice of the present invention. The objectives and other advantages of the present invention may be realized and attained by the following description.

Description of drawings

In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be preferably described in detail below with reference to the accompanying drawings, wherein:

Fig. 1 is the structural representation of the photosynthetic bacteria continuous culture device of the present invention;

Fig. 2 is the assembly drawing of the lamp strip in the photosynthetic bacteria continuous culture device of the present invention;

3 is a schematic structural diagram of a liquid level detector in the photosynthetic bacteria continuous culture device of the present invention;

Fig. 4 is the actual cultivation effect diagram of adopting the photosynthetic bacteria continuous cultivation device of the present invention to cultivate Rhodopseudomonas bog.

Reference numerals: storage tank 1, feed pump 2, feed pump 3, drain pump 4, circulating pump 5, discharge pipe 6, feed pipe 7, culture tank 8, conical bottom 9, support frame 10, upper Cover plate 11, feeding port 12, rotating motor 13, liquid sealing box 14, heat dissipation pipe 15, brush rod 16, return pipe 17, overflow pipe 18, exhaust pipe 19, side wall scale 20, liquid level detector 21 , seed liquid level 22, feed liquid level 23, reaction liquid level 24, warning liquid level 25, programmable controller 26, brush 27, LED light strip 28, transparent sleeve 29, infrared emission window 30, data cable 31. U-shaped slot 32, positioning bolt 33.

Detailed ways

The embodiments of the present invention are described below through specific specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and various details in this specification can also be modified or changed based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the drawings provided in the following embodiments are only used to illustrate the basic idea of the present invention in a schematic manner, and the following embodiments and features in the embodiments can be combined with each other without conflict.

Among them, the accompanying drawings are only used for exemplary description, and represent only schematic diagrams, not physical drawings, and should not be construed as limitations of the present invention; in order to better illustrate the embodiments of the present invention, some parts of the accompanying drawings will be omitted, The enlargement or reduction does not represent the size of the actual product; it is understandable to those skilled in the art that some well-known structures and their descriptions in the accompanying drawings may be omitted.

The same or similar numbers in the drawings of the embodiments of the present invention correspond to the same or similar components; in the description of the present invention, it should be understood that if there are terms “upper”, “lower”, “left” and “right” , "front", "rear" and other indicated orientations or positional relationships are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the indicated device or element must be It has a specific orientation, is constructed and operated in a specific orientation, so the terms describing the positional relationship in the accompanying drawings are only used for exemplary illustration, and should not be construed as a limitation of the present invention. situation to understand the specific meaning of the above terms.

The photosynthetic bacteria continuous culture device shown in Figures 1-3 includes a culture tank 8 and a storage tank 1 arranged outside the culture tank 8. The culture tank 8 is composed of transparent materials such as plexiglass or tempered glass. The bottom of the culture tank 8 is a conical bottom 9, a discharge pipe 6 is installed in the middle of the conical bottom 9 of the culture tank 8, and a feed pipe 7 and a return pipe are respectively fixed on the conical bottom 9 on both sides of the discharge pipe 6. 17. Two branch pipes are connected to the feed pipe 7, one of which is connected to the storage tank 1 through the feed pump 2, and the other branch is connected to the tap water through the feed pump 3. A drain pump 4 is installed on the discharge pipe, and a circulation pump 5 is connected to the return pipe 17. The circulation pump 5 continuously circulates the bacterial liquid in the conical bottom 9 of the culture tank.

A transparent sleeve with a rotary motor 13 is rotatably installed in the middle of the culture tank 8, and the rotation speed of the rotary motor 13 is not greater than 60 r/min. The transparent sleeve 29 is made of transparent materials such as plexiglass or tempered glass. The heat dissipation pipe 15 fixed on the culture tank 8 is placed in the transparent sleeve. The heat dissipation pipe 15 can maintain the temperature of the bacterial liquid in the culture tank 8 . The heat pipe 15 is composed of aluminum-based or copper-based materials, and the interior is hollow. The heat pipe 15 is screwed with an LED light strip 28 , and the illuminance of the LED light strip 28 is in the range of 1500-5000 lux. The LED light strip 28 is spirally wound and adhered to the heat dissipation pipe 15, the transparent sleeve 29 is sleeved outside the heat dissipation pipe 15 with the LED light strip 28, and the liquid sealing box 14 is fixedly installed at the connection between the transparent sleeve 29 and the culture tank 8, The liquid sealing box 14 plays the role of sealing the connection between the transparent sleeve 29 and the culture tank 8 , preventing the culture liquid in the culture tank 8 from entering the transparent sleeve 29 and damaging the heat dissipation pipe 15 and the LED light strip 28 . The heat dissipation pipe 15 and the LED light strip 28 located in the transparent sleeve 29 are not connected to the rotary motor 13 and do not rotate with the rotary motor 13 . The outer side of the transparent sleeve 29 is fixedly installed with 2 to 4 brush rods 16 distributed along the circumferential direction of the transparent sleeve 29, the brush rods 16 are fixedly connected with the culture tank 8, and each brush rod 16 is fixedly installed along the transparent sleeve 29. Several brushes 27 evenly distributed in the radial direction, the free ends of the brushes 27 are in contact with the outer wall of the transparent sleeve 29, and the rotating motor 13 drives the transparent sleeve to rotate so that the outer wall of the transparent sleeve continuously rotates along the brush rod 16, and the remaining parts in the transparent sleeve The photosynthetic bacteria on the outer wall of the tube is scratched to prevent the photosynthetic bacteria from gathering on the outer wall of the transparent casing. In this embodiment, two brush rods 16 are used as an example. The central axes of the two brush rods 16 and the transparent sleeve 29 are on the same straight line, and the thickness of a single brush rod 16 is not greater than 0.5 cm.

The culture tank 8 on the upper part of the conical bottom 9 is embedded with a support frame 10 for supporting the heat dissipation pipe 15 and the transparent sleeve. The top of the culture tank 8 is provided with an upper cover plate 11. The inner wall is sealed and fitted, and the liquid sealing box 14 is installed on the upper cover plate 11 . A feeding port 12 and an exhaust pipe 19 are also installed on the upper cover plate 11 . The middle of the support frame 10 is provided with a circular limit hole that is adapted to the transparent sleeve 29. The upper end of the transparent sleeve 29 is fixed on the bearing of the rotating electrical machine 13, and the lower end penetrates through the support frame 10 and can be within the range of the limit hole. Rotating, the transparent sleeve 29 is driven by the rotating motor 13 to rotate. The transparent sleeve 29 protrudes from the liquid sealing box 14 and is exposed to the outside of the culture tank 8 with 1 to 5 circular holes, which facilitates the hot air in the transparent sleeve 29 to be discharged. The upper end of the brush rod 16 is fixed to the upper cover plate 11 of the culture tank 8 , and the lower end is fixed to the support frame 10 .

A side wall ruler 20 is fixedly installed on the outer wall of the culture tank 8, and 3 to 8 U-shaped clamping grooves 32 are slidably clamped on the side wall ruler 20. Each U-shaped clamping groove 32 is threadedly connected with positioning bolts 33. The liquid level detectors 21 with data lines 31 and infrared emission windows 30 are installed on the U-shaped card slots. The liquid level detectors 21 can slide on the side wall ruler 20 through the corresponding U-shaped card slots. The gauges 21 are all fixed on the side wall scale 20 through the positioning bolts 33 on the corresponding U-shaped grooves, and the infrared emission window 30 of the liquid level detector 21 is closely attached to the cylindrical side wall. The liquid level detector 21 , the feed pump 2 , the feed pump 3 , the circulation pump 5 and the drain pump 4 are connected to the same programmable controller 26 . In this embodiment, four liquid level detectors 21 are used as an example, and the warning liquid level 25 , the reaction liquid level 24 , the feed liquid level 23 and the seed liquid level 22 of the culture tank are sequentially marked from top to bottom. The ratio of the volume corresponding to the seed liquid level 22 to the effective volume of the culture tank 8 is 1:20 to 1:3, and the ratio of the volume corresponding to the feed liquid level 23 to the effective volume of the culture tank 8 is 1:10 to 1:2 . An overflow pipe 18 is installed on the outer wall of the culture tank 8 between the reaction liquid level 24 and the warning liquid level 25 to facilitate the discharge of excess bacterial liquid in the culture tank 8 to the external system.

The intelligent control method of the photosynthetic bacteria continuous culture device specifically includes the following steps:

A, after the photosynthetic bacteria continuous culture device is assembled, manually add the microbial seed liquid to the seed liquid level 22 from the feeding port 12 at the top of the culture tank 8;

B, turn on the intelligent control system, the feed pump 2 is turned on under the control of the programmable controller 26, and the culture medium is pumped from the storage tank 1 to the culture tank 8, and when the feeding liquid level 23 is reached, the feed pump 2 Automatically shut down, the inlet water pump 3 is automatically turned on, when the reaction liquid level 24 is reached, the inlet water pump 3 is automatically turned off, and the circulating pump 5 is automatically turned on. The microorganisms in tank 8 begin to cultivate;

C. After the microorganisms are cultivated to the set time, the LED light strip 28 and the circulation pump 5 in the lighting system are automatically turned off, the drainage pump 4 on the discharge pipe 6 is turned on, and the cultured bacterial liquid is put into the external system. When the surface drops to the seed liquid level 22, the drain pump 4 on the discharge pipe 6 is automatically turned off;

D, feed pump 2 is turned on, repeat step B and step C, carry out the cultivation of photosynthetic bacteria in the next cycle, thereby realize the intelligent control of continuous cultivation and continuous delivery of photosynthetic bacteria, in the process of continuous cultivation of photosynthetic bacteria, the culturing tank 8 A warning liquid level 25 is set on the upper part. When the culture tank 8 fails and the material liquid in it exceeds the warning liquid level 25 , the drainage pump 4 is automatically turned on to drain water, and the drainage pump 4 is turned off until the liquid level drops to the reaction liquid level 24 .

Example

Taking the cultivation of Rhodopseudomonas marsh as an example, the effective volume of the culture tank 8 is 100L, the heat pipe 15 is an aluminum base heat pipe 15, and the LED light strip 28 is a 5055 type LED warm white light strip. The transparent sleeve 29 and the main body culture tank 8 are sealed by the liquid sealing box 14, and the liquid sealing box 14 is filled with tap water. Three bundles of brushes 27 are arranged outside the transparent sleeve 29 .

The culture control method of the continuous culture device for Rhodopseudomonas swamps specifically includes the following steps:

A. After assembling the continuous culture device of Rhodopseudomonas bog, the volume corresponding to 8 liquid levels 22 of the culture tank is set to be 10L, the volume corresponding to the feed liquid level 23 is 20L, and the volume corresponding to the reaction liquid level 24 is 100L, the volume corresponding to the warning liquid level 25 is 102L, the culture time is 72h, the culture period is set to 3 cycles, the storage tank 1 is filled with the concentrated Rhodopseudomonas bog, and the feeding port at the top of the culture tank 8 is manually 12 Add Rhodopseudomonas swamps seed liquid to seed liquid level 22 (10L);

B, open the intelligent control system, the feed pump 2 is opened under the control of the programmable controller 26, and the culture medium is pumped from the storage tank 1 to the culture tank 8, and when the feed liquid level 23 (20L) is reached, the feed The feed pump 2 is automatically turned off, and the feed pump 3 is automatically turned on. When the reaction liquid level 24 (100L) is reached, the feed pump 3 is automatically turned off, and the circulating pump 5 is automatically turned on. The motor 13 is automatically turned on, and the microorganisms in the culture tank 8 begin to be cultivated;

C. After the microorganisms are cultivated to the set time for 72 hours, the LED light strip 28 and the circulation pump 5 in the lighting system are automatically turned off, the drainage pump 4 on the discharge pipe 6 is turned on, and the cultured bacterial liquid is put into the external system. When the liquid level drops to the seed liquid level 22 (10L), the drain pump 4 on the discharge pipe 6 is automatically turned off;

D, feed pump 2 is opened, repeat step B and step C (when the liquid level rises to the feed liquid level 23, the feed pump 2 stops, the feed pump 3 is turned on, and when the liquid level rises to the reaction liquid level 24, feed The water pump 3 is turned off, the circulation pump 5 and the lighting system are turned on) twice to realize the continuous cultivation of R. During the continuous culture of the bacterium, the transparent sleeve 29 is continuously rotated along the brush rod 16 under the driving of the rotating motor 13, and scrapes the Rhodopseudomonas swamps remaining on the outer wall of the transparent sleeve 29 to prevent it from gathering. on the outer wall of the transparent sleeve 29 . The upper part of the culture tank 8 is provided with a warning liquid level 25. When the culture tank 8 fails to make the material liquid in it exceed the warning liquid level 25, the drainage pump 4 is automatically opened to drain water until the liquid level drops to the reaction liquid level 24. When the drainage pump 4 is closed .

The actual culture effect of Rhodopseudomonas marsh is shown in Figure 4. The results show that the present invention can better realize the continuous culture and continuous delivery of Rhodopseudomonas swamps. 3.3 billion /ml, reaching the concentration specified in the NY 527-2002 standard.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be Modifications or equivalent replacements, without departing from the spirit and scope of the technical solution, should all be included in the scope of the claims of the present invention.

Claims (10)

1. A photosynthetic bacteria continuous culture device is characterized by comprising a culture tank and a storage tank arranged outside the culture tank, wherein the bottom of the culture tank is provided with a discharge pipe, two sides of the discharge pipe are respectively and fixedly provided with a feed pipe communicated with the storage tank and a return pipe convenient for the circulation of bacterial liquid in the culture tank, the feed pipe is connected with two branch pipes, one branch is connected with the storage tank through a feed pump, the other branch is connected with tap water through a feed pump, the discharge pipe is provided with a drainage pump, the return pipe is connected with a circulating pump, and the top of the culture tank is provided with a feed inlet and an exhaust pipe;

a transparent sleeve with a rotating motor is rotatably arranged in the middle of the culture tank, a radiating pipe which is fixed on the culture tank and is spirally sleeved with an LED lamp strip is arranged in the transparent sleeve, a liquid seal box is fixedly arranged at the joint of the transparent sleeve and the culture tank, a plurality of brush rods which are circumferentially distributed along the transparent sleeve are fixedly arranged outside the transparent sleeve, the brush rods are fixedly connected with the culture tank, a plurality of brushes which are uniformly distributed along the radial direction of the transparent sleeve are fixedly arranged on each brush rod, and the free ends of the brushes are abutted against the outer wall of the transparent sleeve;

fixed mounting has the lateral wall scale on the cultivation jar outer wall, and sliding connection has a plurality of level detection meter on the lateral wall scale, and level detection meter, charge pump, intake pump, circulating pump and drain pump are connected on same programmable controller.

2. A continuous culture apparatus for photosynthetic bacteria as claimed in claim 1 wherein the culture tank is embedded with a support frame for supporting the heat dissipation pipe and the transparent sleeve, the top of the culture tank is mounted with an upper cover plate which is sealed with the inner wall of the cylindrical culture tank, the liquid seal box is mounted on the upper cover plate, the feed inlet and the exhaust pipe are mounted on the upper cover plate, and the middle of the support frame is provided with a circular limiting hole adapted to the transparent sleeve.

3. A photosynthetic bacteria continuous culture device as claimed in claim 1, wherein the side wall scale is provided with a plurality of U-shaped slots, each U-shaped slot is connected with a positioning bolt by screw thread, each U-shaped slot is provided with a liquid level detector with a data line and an infrared emission window, the liquid level detector slides on the side wall scale through the corresponding U-shaped slot, and the infrared emission window of the liquid level detector is tightly attached to the outer wall of the culture tank.

4. A continuous culture apparatus for photosynthetic bacteria as claimed in claim 2 wherein the upper end of the brush rod is fixed to the upper cover plate of the culture tank and the lower end is fixed to the support frame.

5. A continuous culture device for photosynthetic bacteria as claimed in claim 1 wherein the transparent casing has a plurality of circular holes extending from the liquid-tight box to the outside of the culture tank.

6. A photosynthetic bacteria continuous culture apparatus according to claim 1 wherein the number of the liquid level detectors is 4, and the warning liquid level, the reaction liquid level, the feed liquid level and the liquid level are indicated in this order from top to bottom, the ratio of the volume corresponding to the liquid level to the effective volume of the culture tank is 1:20 to 1:3, and the ratio of the volume corresponding to the feed liquid level to the effective volume of the culture tank is 1:10 to 1: 2.

7. A continuous culture apparatus for photosynthetic bacteria as claimed in claim 6 wherein the overflow pipe is installed on the outer wall of the culture tank between the reaction liquid level and the warning liquid level.

8. A photosynthetic bacteria continuous culture apparatus according to claim 1, wherein the bottom of the culture tank is a tapered bottom, and the heat radiating pipe is made of an aluminum-based or copper-based material and has a hollow interior.

9. A photosynthetic bacteria continuous culture device according to claim 1, characterized in that the culture tank and the transparent casing are made of organic glass or toughened glass.

10. A method for controlling a continuous culture apparatus for photosynthetic bacteria according to any one of claims 1 to 9, comprising the steps of:

A. after the photosynthetic bacteria continuous culture device is assembled, microorganism liquid is manually added to the liquid level from a feed inlet at the top of the culture tank;

B. the intelligent control system is started, the feeding pump is started under the control of the programmable controller, the culture medium is pumped from the storage tank to the culture tank, when the liquid supplement level is reached, the feeding pump is automatically closed, the water inlet pump is automatically started, when the reaction level is reached, the water inlet pump is automatically closed, the circulating pump is automatically started, after the circulating pump is started, the LED lamp strip and the rotating motor in the lighting system are automatically started, and the microorganisms in the culture tank start to be cultured;

C. after the microorganisms are cultured for a set time, the LED lamp strip and the circulating pump in the lighting system are automatically closed, the drainage pump on the discharge pipe is opened, the cultured bacterial liquid is put into an external system, and when the liquid level of the bacterial liquid drops to the liquid level of the seed liquid, the drainage pump on the discharge pipe is automatically closed;

D. and C, starting a feeding pump, repeating the step B and the step C, and culturing the photosynthetic bacteria in the next period, so as to realize intelligent control of continuous culture and continuous feeding of the photosynthetic bacteria.

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