CN112042835A - A kind of non-full nutrition special medical food with hypolipidemic effect and preparation method thereof - Google Patents

Abstract

The invention provides a non-full nutrition special medical food with hypolipidemic effect and a preparation method thereof, belonging to the technical field of special medical food. The special medical food is prepared from the following raw materials by weight: 20-70 parts of red yeast rice vinegar, 30-60 parts of fresh fruit and vegetable juice, 20-50 parts of Chinese herbal medicine extract, 3-5 parts of xylitol, 0.001-0.01 part of potassium sorbate, 0.01-0.05 part of Lactobacillus plantarum. The invention uses red yeast rice vinegar and Lactobacillus plantarum as raw materials to produce special medical food, which has health-care functions such as lowering blood pressure and blood lipid, and has strong pertinence. Using celery, onion, garlic, mushroom, yam, apple, kiwi juice and cassia seed, hawthorn, lotus leaf, honeysuckle, pueraria, chrysanthemum, green tea extract and red yeast rice vinegar stock solution for deployment, the taste is good, the flavor is special, At the same time, the lipid-lowering activity of red yeast rice vinegar was enhanced.

Description

A kind of non-full nutrition special medical food with hypolipidemic effect and preparation method thereof

technical field

The invention belongs to the technical field of special medical food, and in particular relates to a non-full nutrition special medical food with hypolipidemic effect and a preparation method thereof.

Background technique

Foods for special medical purposes, referred to as food for special medical purposes, are formula foods specially processed and prepared to meet the special needs of people with complete or partial food restriction, digestive and absorption disorders, metabolic disorders or specific disease states for nutrients or diets. Special medical food is a new type of product that is different from ordinary food, health products and medicines. It is a type of food with special food purposes that patients who need special food management take under the guidance of doctors. Non-full nutrition food for special medical purposes is a formula food for special medical purposes that can meet some of the nutritional needs of the target population, but long-term single use will cause malnutrition, so it should be used together with ordinary food or other nutritional measures.

Chinese patent document CN109294863A discloses a kind of millet monascus health-care vinegar and its preparation method. It is prepared from 15-30 servings of Mujiang Ye Ke, etc. The health-care vinegar has the functions of unique taste and flavor and high nutritional value, but its nutrition is slightly less targeted.

SUMMARY OF THE INVENTION

In order to solve the problem of high blood lipid level in hyperlipidemia patients, the present invention provides a non-full nutrition formula food for special medical purposes with blood lipid lowering effect.

The present invention proposes a kind of non-full nutrition special medical food with hypolipidemic effect, which is prepared from raw materials including the following parts by weight:

20-70 parts of red yeast rice vinegar, 30-60 parts of fresh fruit and vegetable juice, 20-50 parts of Chinese herbal medicine extract, 3-5 parts of xylitol, 0.001-0.01 part of potassium sorbate, and 0.01-0.05 part of Lactobacillus plantarum.

Further, it is prepared from the raw material comprising the following parts by weight:

30-60 parts of red yeast rice vinegar, 40-50 parts of fresh fruit and vegetable juice, 30-40 parts of Chinese herbal medicine extract, 4-5 parts of xylitol, 0.001-0.005 part of potassium sorbate, and 0.01-0.03 part of Lactobacillus plantarum.

Further, the Lactobacillus plantarum, strain number CICC 24435, was purchased from China Industrial Microorganism Culture Collection and Management Center.

Further, described fresh fruit and vegetable juice is prepared from the raw material that comprises following parts by weight:

20-30 servings of celery, 5-10 servings of onion, 1-5 servings of garlic, 25-35 servings of yam, 5-15 servings of shiitake mushroom, 25-35 servings of apple, and 10-20 servings of kiwi.

Further, described fresh fruit and vegetable juice is prepared from the raw material that comprises following parts by weight:

20-25 servings of celery, 5-8 servings of onion, 1-3 servings of garlic, 25-30 servings of yam, 5-10 servings of shiitake mushroom, 25-30 servings of apple, and 10-15 servings of kiwi.

Further, the Chinese herbal medicine extract is prepared from the raw materials comprising the following parts by weight:

15-35 parts of cassia seed, 20-50 parts of hawthorn, 10-30 parts of lotus leaf, 5-15 parts of honeysuckle, 10-40 parts of pueraria, 20-30 parts of chrysanthemum, 25-35 parts of green tea.

Further, the Chinese herbal medicine extract is prepared from the raw materials comprising the following parts by weight:

25 parts of cassia seed, 35 parts of hawthorn, 20 parts of lotus leaf, 10 parts of honeysuckle, 35 parts of pueraria, 25 parts of chrysanthemum, 30 parts of green tea.

The present invention also proposes the above-mentioned preparation method, comprising the following steps:

(1) prepare fresh fruit and vegetable juice: select fresh celery, onion, garlic, shiitake mushroom, yam, apple, kiwi after cleaning and peeling, filter and separate after squeezing the juice respectively, obtain juice and pomace, the gained juice is mixed and refrigerated for later use, The obtained pomace is dried and pulverized and sieved for subsequent use;

(2) preparation of Chinese herbal medicine extract: select cassia seed, hawthorn, lotus leaf, honeysuckle, pueraria, chrysanthemum, green tea, pick out impurities, slice and dry in the sun, after mixing, soak in boiling water, and the obtained filtrate is refrigerated for later use;

(3) processing of raw material: after red yeast rice vinegar is mixed with the pomace obtained in step (1), Lactobacillus plantarum is added for fermentation and culture to obtain fermented product;

(4) Allocate: add fresh fruit and vegetable juice, Chinese medicinal material extract and xylitol to the fermented product obtained in step (3), and deploy to obtain a product.

Further, in step (2), the temperature of the boiling water is 100°C.

Further, it also includes the following steps (5) bottling and sterilizing: quantitatively bottling, capping and sterilizing the prepared product obtained in step (4).

Further, in step (1), the drying temperature is 40-60° C.; the drying time is 15-25 h.

The present invention has the following advantages:

(1) The present invention uses red yeast rice vinegar and Lactobacillus plantarum as raw materials to produce special medical food, which has health care functions such as lowering blood pressure and blood lipid, and is highly targeted.

Using red yeast rice vinegar, Lactobacillus plantarum and other raw materials, after fermenting and culturing, adding fresh vegetables and fruits, Chinese herbal medicine extract and xylitol to prepare the special medical food with hypolipidemic effect. The special medical food with hypolipidemic effect prepared by the above steps is rich in organic acids, which can promote the metabolism of the human body and the redox of cells. Lactobacillus plantarum used in the fermentation process can produce lipid-lowering components such as cellulose pectinase, which has the effect of assisting in lowering blood lipids. Combined with traditional Chinese medicine extracts and fruit and vegetable juices that have a coordinated lipid-lowering effect, it can increase the effect of red yeast rice vinegar on hyperlipidemia. The therapeutic effect of the disease.

(2) the present invention uses the juice of celery, onion, garlic, shiitake mushroom, yam, apple, kiwi fruit, and the extract of cassia, hawthorn, lotus leaf, honeysuckle, pueraria, chrysanthemum, green tea and red yeast rice vinegar stock solution for deployment, and the taste is good , the flavor is special, and the lipid-lowering activity of red yeast rice vinegar is enhanced. Among them, the added xylitol can accelerate the synthesis of liver glycogen and the consumption of protein and fat, and has the functions of improving liver health, preventing dental caries, losing weight, and stabilizing insulin levels.

The added fruit and vegetable juice and fruit and vegetable residues are made from celery, onion, garlic, apple, kiwi, yam, and shiitake, and the extract of Chinese herbal medicine is made from cassia seeds, hawthorn, lotus leaves, honeysuckle, pueraria, chrysanthemum, green tea It is made by soaking in boiling water after drying the raw material slices. Fruits are rich in vitamin C, which can promote the metabolism of body fat; vegetables are rich in vitamins, chlorophyll and its derivatives, and a large amount of dietary fiber, which can lower cholesterol and increase high-density lipoprotein cholesterol in the blood. Prevent arteriosclerosis; green tea can reduce blood lipids, promote blood circulation, and prevent arteriosclerosis; cassia seeds contain anthracene glycosides, which can be decomposed to produce rhein, emodin, chrysophanol, glucose, etc., as well as vitamin A and other ingredients, which can lower blood pressure , The effect of lowering blood lipids; hawthorn, chrysanthemum, honeysuckle have the effects of lowering blood lipids, clearing heat and detoxifying. The extracted fruit and vegetable juice and Chinese herbal medicine extracts are added to red yeast rice vinegar, which can enhance the lipid-lowering effect of red yeast rice vinegar, and can prevent and treat hyperlipidemia.

(3) the present invention carries out fermentation culture to red yeast rice vinegar and pomace by adding Lactobacillus plantarum simultaneously through various raw materials and optimized process conditions, and the obtained lipid-lowering red yeast rice vinegar not only has the unique fragrance of red yeast rice vinegar, but also It has the fragrance of fruits and vegetables, and at the same time enhances the lipid-lowering activity of lipid-lowering red yeast rice vinegar.

Detailed ways

It should be noted that the embodiments of the present invention and the features of the embodiments may be combined with each other under the condition of no conflict.

Embodiment 1 A kind of preparation method of special medical food with hypolipidemic effect

The special medical food is prepared from the raw materials including the following parts by weight:

55 parts of red yeast rice vinegar, 45 parts of fresh fruit and vegetable juice, 35 parts of Chinese herbal medicine extract, 4.5 parts of xylitol, 0.001 part of potassium sorbate, and 0.03 part of Lactobacillus plantarum.

The preparation method of the special medical food comprises the following steps:

(1) Preparation of fresh fruit and vegetable juice: Choose 25 parts of fresh celery, 8 parts of onion, 3 parts of garlic, 30 parts of yam, 10 parts of shiitake mushrooms, 30 parts of apples, and 15 parts of kiwi fruit. After cleaning and peeling, use a juicer to separate them. After the juice is squeezed, it is separated by filtration to obtain the juice and pomace of the vegetables and fruits. The juice of the vegetables and fruits is mixed according to the proportion of raw materials and then refrigerated for later use. ;

(2) Preparation of Chinese herbal medicine extract: select 25 parts of high-quality cassia seeds, 35 parts of hawthorn, 20 parts of lotus leaves, 10 parts of honeysuckle, 35 parts of pueraria, 25 parts of chrysanthemum, and 30 parts of green tea, pick out impurities, slice and sun-dry, After mixing according to the raw material ratio of the Chinese herbal medicine extract, soak it in boiling water for 30 minutes, filter it with a filter screen, and take the filtrate to refrigerate for later use;

(3) raw material treatment: after mixing the red yeast rice vinegar and the pomace obtained in step (1) according to an appropriate ratio, adding Lactobacillus plantarum, 30 ° C, under pH 6 conditions, anaerobic culture for 15 hours for subsequent use;

(4) deployment: adding the red yeast rice vinegar obtained in step (3) into fresh fruit and vegetable juice, Chinese medicinal material extract and xylitol, and deploying;

(5) Bottling and sterilization: quantitatively bottling and capping the prepared red yeast rice vinegar, and sterilizing by pasteurization for 10 minutes.

Embodiment 2 A kind of preparation method of special medical food with hypolipidemic effect

Same as Example 1, the difference is that the addition amount of Lactobacillus plantarum is 0.02 part. In step (3), after adding Lactobacillus plantarum, anaerobic culture is carried out for 16 hours at a temperature of 36° C. and a pH of 6.5.

Embodiment 3 A kind of preparation method of special medical food with hypolipidemic effect

The same as Example 2, the difference is that 65 parts of red yeast rice vinegar, 40 parts of fresh fruit and vegetable juice, 30 parts of Chinese herbal medicine extract, 4.5 parts of xylitol, 0.001 part of potassium sorbate, and 0.02 part of Lactobacillus plantarum.

It should be pointed out that the Lactobacillus plantarum described in Examples 1-3, the strain number CICC 24435, was purchased from the China Industrial Microorganism Culture Collection and Management Center. Red yeast rice vinegar was purchased from Shandong Yutu Food Co., Ltd.

Comparative Example 1 A kind of preparation method of special medical food with hypolipidemic effect

With embodiment 1, the difference is that in step (3), Lactobacillus plantarum is not added.

Experimental example 1 Determination of product hygienic quality

The products prepared in Example 1 of the present invention were tested for quality indicators such as microorganisms and heavy metals, as well as hygienic indicators. The results are shown in Table 1, which all meet the national safety standards.

Table 1 Product sanitation quality test results

Experimental Example 2 Determination of the lipid-lowering effect of the product

Experiments were carried out with the lipid-lowering effects of the finished products prepared in Examples 1, 2, 3 and Comparative Example 1, as follows:

(1) Experimental animals and feed: healthy male SD rats, weighing 180±10 g, were purchased from Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd. Sterile bedding and feed were purchased from Beijing Keao Xieli Feed Co., Ltd. Under the standard rearing conditions of 12 hours of light and 12 hours of dark cycles, they were normally raised in the Animal Experiment Center (SPF), School of Bioengineering, Tianjin University of Science and Technology. The indoor ventilation conditions were good, the relative humidity was 55 ± 5%, and the temperature was 23 ± 2 °C . Experiments were started after one week of adaptive feeding. (Ethical statement: Male SD rats (6 weeks old, body weight 180±10 g) were purchased from Beijing Weitong Lihua Laboratory Animal Technology Co., Ltd. (Beijing, China). (License No.: SCXK (Beijing) 2014-0013) Animals Performed in accordance with National Institutes of Health guidelines, experiments were approved by the Animal Ethics Committee of Tianjin University of Science and Technology).

Regular feed: carbohydrates: 59%, protein: 21.1%, fiber: 4.9%, fat: 4.2%, ash: 8%, phosphorus: 1%, calcium: 1.8%.

High-fat feed: 2% cholesterol + 10% lard + 0.2 pig bile salt + 10% egg yolk powder + 5% sugar + conventional feed.

Products: The experimental groups are the products prepared in Examples 1, 2, 3 and Comparative Example 1.

(2) Model establishment and administration method

Blood lipid metabolism disorder-treatment animal model is established by feeding animals with high-fat diet to form experimental lipid metabolism disorders and lesions similar to the early stage of human beings, and at the same time adopting the treatment mode of administration, random grouping according to no significant difference in average body weight, blank The group was fed with conventional feed, the model group was fed with high-fat feed, and the rest of the experimental groups were fed with "high-fat feed + product" mode.

SD rats were fed regular diet for one week to adapt to the environment, during which they had free access to food and water, and adapted to a regular cycle of 12h day/12h dark night. Then they were randomly divided into 5 groups according to their body weight and were reared for 12 weeks. For grouping and dosage, see Table 2.

Blank group (Vehicle): conventional feed feeding;

Model group (Model): high-fat diet feeding;

Low-dose group (LD): 1 mL/kg/day of the product (adults 10 mL/70 kg/day) after feeding with high-fat diet for several days;

High-dose group (HD): 3 mL/kg/day of the product (adult 30 mL/70 kg/day) after feeding with high-fat feed for several days;

Positive control group (PC): Lovastatin 2 mg/kg/day (adult 20 mg/70 kg/day) after feeding with high-fat diet for several days;

Negative control group (NC): After being fed with high-fat diet for several days, 3 mL/kg/day of acetic acid was intragastrically administered, and the concentration of acetic acid was 4 g/100 mL.

Table 2

(3) Collection and determination of biological samples

During the establishment of the lipid metabolism disorder-treatment model, the body weight was weighed once a week, the changes were recorded, and orbital blood was collected every two weeks to determine the serum TC, TG, HDL-C, and LDL-C levels in each group. The experiment was carried out to the 12th week after completion of the last intragastric administration, and animals in each group were fasted for 16 hours and had free access to water. After weighing the body weight the next day, the test animals were anesthetized by intraperitoneal injection of 10% chloral hydrate, blood was collected from the heart, and the collected blood was collected with blood collection tubes. The collected serum was frozen at -80°C for future use. After the blood collection was completed, the animals were killed by decapitation, and the organs for follow-up research were quickly dissected and stripped. Immediately, the blood was removed by gentle washing with 4°C normal saline to remove the blood indicated by the organs, and frozen in aliquots for subsequent research.

Blood lipid indexes, including serum TC, TG, HDL-C and LDL-C, were measured using kits purchased by Nanjing Jiancheng Bioengineering Institute, and related operations and results were calculated according to the instructions of the kits.

(4) Data analysis

Each sample was repeated at least 3 times, and the data were expressed as mean ± standard deviation (x ± SD). Analysis of variance and correlation analysis were performed, and p<0.05 was considered a significant difference.

Table 3 Changes in body weight of rats

Note: Compared with the blank group, p < 0.01, the difference is extremely significant; compared with the control group, the experimental group and the model group, p < 0.001, the difference is extremely significant.

Table 4 Changes of blood lipid indexes in rats

Note: Compared with the blank group, p < 0.05, the difference is significant; compared with the positive control group, p < 0.05, the difference is significant; compared with the negative control group, p < 0.01, the difference is extremely significant; Compared with the model group, p < 0.01, the difference is extremely significant.

As shown in Tables 3 and 4, in Examples 1, 2, and 3, due to the addition of fruit and vegetable juice, Chinese herbal medicine extracts, Lactobacillus plantarum, etc., it was verified by animal experiments that they had obvious hypolipidemic effects. TC, TG in serum Compared with the model group, the LDL-C index was significantly reduced, and the HDL-C was significantly increased; wherein, in Example 2, due to the optimization of the Lactobacillus plantarum fermentation conditions, the lipid-lowering effect of the obtained product was better, and the body weight of the rats was significantly reduced. ; In Comparative Example 1, because Lactobacillus plantarum was not added, the lipid-lowering activity of the obtained product was low, and the body weight of the rats increased significantly.

The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention. within.

Claims (9)

1. A non-total nutrient special medical food with the function of reducing blood fat is characterized in that,

the composite material is prepared from the following raw materials in parts by weight:

20-70 parts of red yeast rice vinegar, 30-60 parts of fresh fruit and vegetable juice, 20-50 parts of a traditional Chinese medicine extracting solution, 3-5 parts of xylitol, 0.001-0.01 part of potassium sorbate and 0.01-0.05 part of lactobacillus plantarum.

2. The specialist food according to claim 1, wherein,

the composite material is prepared from the following raw materials in parts by weight:

30-60 parts of red yeast rice vinegar, 40-50 parts of fresh fruit and vegetable juice, 30-40 parts of a traditional Chinese medicine extracting solution, 4-5 parts of xylitol, 0.001-0.005 part of potassium sorbate and 0.01-0.03 part of lactobacillus plantarum.

3. The specialist food according to claim 1, wherein,

the fresh fruit and vegetable juice is prepared from the following raw materials in parts by weight:

20-30 parts of celery, 5-10 parts of onion, 1-5 parts of garlic, 25-35 parts of Chinese yam, 5-15 parts of mushroom, 25-35 parts of apple and 10-20 parts of kiwi fruit.

4. The specialist food according to claim 1, wherein,

the fresh fruit and vegetable juice is prepared from the following raw materials in parts by weight:

20-25 parts of celery, 5-8 parts of onion, 1-3 parts of garlic, 25-30 parts of Chinese yam, 5-10 parts of mushroom, 25-30 parts of apple and 10-15 parts of kiwi fruit.

5. The specialist food according to claim 1, wherein,

the traditional Chinese medicine extracting solution is prepared from the following raw materials in parts by weight:

15-35 parts of cassia seed, 20-50 parts of hawthorn, 10-30 parts of lotus leaf, 5-15 parts of honeysuckle, 10-40 parts of kudzu root, 20-30 parts of chrysanthemum and 25-35 parts of green tea.

6. The specialist food according to claim 1, wherein,

the traditional Chinese medicine extracting solution is prepared from the following raw materials in parts by weight:

25 parts of cassia seed, 35 parts of hawthorn, 20 parts of lotus leaf, 10 parts of honeysuckle, 35 parts of kudzu root, 25 parts of chrysanthemum and 30 parts of green tea.

7. The method for preparing the non-total nutrient special medical food with the hypolipidemic effect according to any one of claims 1 to 6, wherein the method comprises the following steps:

(1) preparing fresh fruit and vegetable juice: selecting fresh celery, onion, garlic, mushroom, Chinese yam, apple and kiwi fruit, cleaning, peeling, respectively juicing, filtering and separating to obtain juice and pomace, mixing the obtained juice, refrigerating for later use, drying the obtained pomace, crushing and sieving for later use;

(2) preparing a traditional Chinese medicine extracting solution: selecting semen Cassiae, fructus crataegi, folium Nelumbinis, flos Lonicerae, radix Puerariae, flos Chrysanthemi, and green tea, removing impurities, slicing, sun drying, mixing, soaking in boiling water, and refrigerating the filtrate;

(3) treatment of raw materials: mixing the red yeast rice vinegar with the pomace obtained in the step (1), adding lactobacillus plantarum, fermenting and culturing to obtain a fermented product;

(4) blending: and (4) adding fresh fruit and vegetable juice, the traditional Chinese medicine extracting solution and xylitol into the fermentation product obtained in the step (3), and blending to obtain the product.

8. The method according to claim 7,

also comprises the following steps (5) of bottling and sterilizing: quantitatively bottling the blended product obtained in the step (4), sealing, and sterilizing.

9. The method according to claim 7,

in the step (1), the drying temperature is 40-60 ℃; the drying time is 15-25 h.