CN111979094A - Nucleic acid detection device - Google Patents

Abstract

The present invention provides a nucleic acid detection device, comprising: a rack, with a sample placement position; an image collector, arranged on the rack and above the sample placement position; a light source assembly, arranged on the rack and positioned above the sample placement position; is located above the sample placement position; the light source assembly is used for illuminating a light source only used to excite fluorescence; the shading member is at least arranged around the light emitting surface of the light source assembly and the outer circumference of the lens of the image collector, And the bottom of the light-shielding member at least partially extends to the outer periphery of the sample placement position. With the configuration of this technical solution, a certain number of samples can be placed in the sample placement position. Under the premise of ensuring sensitivity, the detection time of conventional nucleic acid detection methods can be greatly shortened, and it is suitable for large-scale nucleic acid detection application scenarios; at the same time, the setting of light-shielding components The noise of the fluorescent signal can be reduced, the accuracy of the PCR reaction product amount reflected by the fluorescent signal is improved, and the detection accuracy of the nucleic acid detection device is improved.

Description

A nucleic acid detection device

technical field

The present invention relates to the technical field of medical detection, in particular to a nucleic acid detection device.

Background technique

With the outbreak of the global epidemic and the increase of imported cases abroad, new requirements have been put forward for my country's epidemic prevention and control situation. At the same time, the group of asymptomatic infected persons increases the difficulty of epidemic prevention and control. Efficient and reliable pathogen detection methods and devices have become important tools for the prevention and control of infectious diseases.

Routine nucleic acid testing requires a standard PCR laboratory, with professional nucleic acid testing personnel using multi-step instruments and manual operations. In the prior art, as disclosed in Chinese patent document CN210215385U, a nucleic acid detection device includes a temperature-controlled stage, a camera lens and a light source arranged above the stage. A cover plate is arranged on the top of the object stage, a sample accommodating cavity is enclosed between the cover plate and the object stage, and a plurality of detection holes are arranged on the cover plate. The camera lens and the light source are both arranged above the cover plate; the light source is arranged on the outer circumference of the camera lens through the mounting frame; the top of the camera lens is provided with a moving device, which is used to drive the camera lens to move in the X-axis, Y-axis and Z-axis directions , to change the position of the camera lens and light source.

The above-mentioned camera lens and light source move under the drive of the transfer device to align with each detection hole, the light source irradiates the sample to excite the fluorescent chemical substances added in the sample, and the camera lens collects images, and then obtains fluorescence through subsequent processing. Intensity signal curve, the higher the fluorescence signal intensity, the more accumulated PCR reaction products. At present, in order to reduce the noise of the collected fluorescence intensity signal, a filter is usually set at the light-emitting surface of the light source, so that the light source irradiating the sample is only light that can excite fluorescence, and other impurity light is filtered out.

The above-mentioned nucleic acid detection device, although a filter is set on the light-emitting surface of the light source to filter out impurity light, the surrounding of the light source is in the external environment, the light source is illuminated on the path of the detection hole, and the light in the external environment will be doped. In the light source, the sample in the detection hole is irradiated at the same time, resulting in noise in the obtained fluorescence intensity signal, which cannot more accurately reflect the amount of the product in the PCR reaction. That is, the background noise light content is high when collecting the fluorescent signal, which affects the accuracy of the fluorescence intensity signal reflecting the amount of the PCR reaction product, and ultimately leads to low detection accuracy of the nucleic acid detection device.

Therefore, it is necessary to propose an improved nucleic acid detection device.

SUMMARY OF THE INVENTION

Therefore, the technical problem to be solved by the present invention is that the background noise light content of the nucleic acid detection device in the prior art is high during the fluorescence signal collection process, which affects the accuracy of the PCR reaction product amount reflected by the fluorescence signal, resulting in the nucleic acid detection device. The detection accuracy is low.

To this end, the present invention provides a nucleic acid detection device, comprising:

Rack, with sample placement;

an image collector, arranged on the rack and above the sample placement position;

a light source assembly, arranged on the rack and above the sample placement position; the light source assembly is used for irradiating a light source only for exciting fluorescence;

The light-shielding member is arranged around at least the light-emitting surface of the light source assembly and the outer circumference of the lens of the image acquisition device, and the bottom of the light-shielding member at least partially extends to the outer circumference of the sample placement position.

Further, the shading member is a cover body with an opening at the bottom, and the top of the cover body is covered above the tops of the image collector and the light source assembly.

Further, the shading member is vertically slidably arranged on the frame through a lifting mechanism.

Further, the lifting mechanism includes a lifting member fixed on the shading member, and a first driving component that drives the lifting member to perform a lifting motion and is disposed on the frame.

Further, at least one light absorbing layer is provided on the outer wall surface and/or the inner wall surface of the light shielding member.

Further, the nucleic acid detection device further comprises a sample box arranged on the sample placement position; the sample is arranged on the sample placement position by being arranged in the sample box; the top of the sample box is provided with a sample box. One-to-one detection port corresponding to the sample in it;

The bottom of the light shielding member is at least partially wound around the outer circumference of the sample box.

Further, the sample box includes:

The lower cover has an accommodating cavity;

The upper cover is movably arranged on the top opening of the lower cover driven by the first driver, and when the upper cover is fastened on the lower cover, it is enclosed with the lower cover. The upper cover plate is provided with the detection port.

Further, at least one heating element is respectively provided on the inner wall surfaces of the upper cover plate and the lower cover plate; a temperature detector and a controller electrically connected to both the temperature detector and the heating element; and/or the upper cover At least one layer of heat-resistant coating is provided on the outer wall of the board and/or the lower cover.

Further, at least one heat-conducting plate is also provided on the side surface of the heating element facing away from the cover plate where it is located; For PCR plate embedding.

Further, the shading member is sleeved on the outer periphery of the frame; the side wall of the shading member is provided with a sample inlet that communicates with the sample placement position;

The nucleic acid detection device further includes a sample introduction mechanism, and the sample box is provided on the sample introduction mechanism and is driven by the sample introduction mechanism to slide in a first direction to extend into or out of the sample inlet through the sample introduction port. a sample placement location; and sliding at the sample placement location in a second direction perpendicular to the first direction.

Further, the sample introduction mechanism includes:

The first sliding mechanism is arranged on the frame and performs reciprocating linear sliding along the first direction;

a second sliding mechanism, which is arranged on the first sliding mechanism and performs reciprocating direct sliding along the second direction;

A stage is provided on the second sliding member of the second sliding mechanism; the sample box is mounted on the top surface of the stage.

The technical scheme of the present invention has the following advantages:

1. The nucleic acid detection device provided by the present invention comprises:

Rack, with sample placement;

an image collector, arranged on the rack and above the sample placement position;

a light source assembly, arranged on the rack and above the sample placement position; the light source assembly is used for irradiating a light source only for exciting fluorescence;

The light-shielding member is arranged around at least the light-emitting surface of the light source assembly and the outer circumference of the lens of the image acquisition device, and the bottom of the light-shielding member at least partially extends to the outer circumference of the sample placement position. Through the setting of the above technical solutions, a certain number of samples can be placed in the sample placement position, which is easy to achieve high-throughput sample detection. On the premise of ensuring sensitivity, the detection time of conventional nucleic acid detection methods can be greatly shortened, and it is suitable for large-scale nucleic acid detection. At the same time, the setting of the shading component can reduce the noise of the fluorescent signal, improve the accuracy of the amount of PCR reaction product reflected by the fluorescent signal, and improve the detection accuracy of the nucleic acid detection device.

2. In the nucleic acid detection device provided by the present invention, the light-shielding member is a cover body with an opening at the bottom, and the top of the cover body is covered above the tops of the image collector and the light source assembly. Through the arrangement of the above technical solutions, the light shielding component can shield the light emitted by the light source assembly and the image acquisition device, thereby reducing the noise of the fluorescent signal.

3. The nucleic acid detection device provided by the present invention, wherein the light-shielding member is vertically slidably arranged on the rack through a lifting mechanism. Through the arrangement of the above technical solution, when there is a sample box to be detected, a part of the light-shielding member can slide upward to facilitate the entry of the sample box.

4. In the nucleic acid detection device provided by the present invention, the lifting mechanism includes a lifting member fixed on the light-shielding member, and a first driving component that drives the lifting member to perform a lifting motion and is provided on the frame. Through the arrangement of the above technical solutions, the shading component can be automatically moved relative to the frame, which is convenient for control.

5. The nucleic acid detection device provided by the present invention, wherein at least one light-absorbing layer is provided on the outer wall surface and/or the inner wall surface of the light shielding member. Specifically, the light absorbing layer is aluminum black oxide. Through the arrangement of the above technical solutions, the light absorbing layer can absorb light and prevent the reflection of light, and can obtain lower background noise during fluorescence signal detection, reduce the noise of the fluorescence signal, and improve the detection accuracy of the nucleic acid detection device.

6. The nucleic acid detection device provided by the present invention, the nucleic acid detection device further comprises a sample box arranged on the sample placement position; the sample is arranged on the sample placement position by being arranged in the sample box; The top of the sample box is provided with a one-to-one detection port corresponding to the samples in it; the bottom of the light-shielding member is at least partially arranged around the outer circumference of the sample box. Through the arrangement of the above technical solutions, when the sample box is detected, the light-shielding member can shield the outer periphery of the sample box, further shielding the external stray light, lower background noise can be obtained when the fluorescent signal is detected, the noise of the fluorescent signal can be reduced, and the The detection accuracy of the nucleic acid detection device is improved.

7. The nucleic acid detection device provided by the present invention, the sample box comprises:

The lower cover has an accommodating cavity;

The upper cover is movably arranged on the top opening of the lower cover driven by the first driver, and when the upper cover is fastened on the lower cover, it is enclosed with the lower cover. The upper cover plate is provided with the detection port. Specifically, the upper cover can be flipped and connected to the top side of the lower cover, or the upper cover can be slid back and forth on the top of the lower cover, as long as the upper cover can be movably connected to the lower cover The purpose of the present invention can be achieved by the above-mentioned technical solutions, which facilitates the placement and detection of the sample box, and also facilitates the control of the opening and closing of the upper cover.

8. In the nucleic acid detection device provided by the present invention, at least one heating element is respectively provided on the inner wall surfaces of the upper cover plate and the lower cover plate; a temperature detector and a controller electrically connected to both the temperature detector and the heating element; And/or at least one layer of heat-resistant coating is provided on the outer wall of the upper cover plate and/or the lower cover plate. The temperature of the upper cover plate and the lower cover plate is respectively sensed by the corresponding temperature detector, and the controller controls the corresponding heating element to heat, so as to avoid the phenomenon of sample condensation, thereby ensuring the accuracy of the detection result. The heat-resistant coating is a black high polymer, such as black polyoxymethylene (POM), black nylon material, etc., which can not only prevent the side of the sample box facing away from the PCR plate from being overheated and scald the staff, and at the same time, the black coating can The light source assembly does not generate reflection when irradiating, and can obtain lower background noise during fluorescence signal detection, reduce the noise of the fluorescence signal, and improve the detection accuracy of the nucleic acid detection device.

9. In the nucleic acid detection device provided by the present invention, at least one heat conduction plate is further provided on the side surface of the heating element facing away from the cover plate where it is located; or at least one placement concave is arranged in the accommodation cavity of the lower cover plate. groove, the placement groove is used for embedding the PCR plate. Through the arrangement of the above technical solutions, the arrangement of the heat-conducting plate further facilitates heat conduction, and the placement of the groove facilitates the embedding of the PCR plate, thereby facilitating its alignment detection.

10. The nucleic acid detection device provided by the present invention, wherein the light-shielding component is sleeved on the outer periphery of the rack; the side wall of the light-shielding component is provided with a sample inlet that communicates with the sample placement position;

The nucleic acid detection device further includes a sample introduction mechanism, and the sample box is provided on the sample introduction mechanism and is driven by the sample introduction mechanism to slide in a first direction to extend into or out of the sample inlet through the sample introduction port. a sample placement location; and sliding at the sample placement location in a second direction perpendicular to the first direction. Through the arrangement of the above technical solutions, the shading member can shield most of the stray light, and at the same time, the overall sample injection of the sample box can be realized, and the alignment of the PCR plate during detection is also facilitated.

11. The nucleic acid detection device provided by the present invention, the sample introduction mechanism comprises:

The first sliding mechanism is arranged on the frame and performs reciprocating linear sliding along the first direction;

a second sliding mechanism, which is arranged on the first sliding mechanism and performs reciprocating direct sliding along the second direction;

A stage is provided on the second sliding member of the second sliding mechanism; the sample box is mounted on the top surface of the stage. Through the setting of the above technical solutions, the sample injection process of the sample box is realized, the stability of the sample injection process is ensured, and the quality of the samples and the detection results are further ensured.

Description of drawings

In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the accompanying drawings required in the description of the specific embodiments or the prior art. Obviously, the accompanying drawings in the following description The drawings are some embodiments of the present invention. For those of ordinary skill in the art, other drawings can also be obtained from these drawings without creative efforts.

Fig. 1 is the schematic diagram of the nucleic acid detection device in the present invention;

Fig. 2 is the schematic diagram after hiding the shading part in Fig. 1;

Fig. 3 is the schematic diagram that the sample box in Fig. 2 is moved under the light source assembly;

Fig. 4 is the schematic diagram of shading member in one direction;

5 is a schematic diagram of the cooperation of the sample box and the sampling mechanism in the present invention;

Fig. 6 is the schematic diagram of the sample box in Fig. 5;

7 is a schematic diagram of a drive member of the sample box in an embodiment of the present invention;

8 is a schematic diagram of a drive member of a sample box in another embodiment of the present invention;

Reference number:

In the picture: 100-nucleic acid detection device, 1-sample box, 11-detection port, 111-rotating motor, 112-reducer, 113-belt wheel, 12-upper cover, 13-lower cover, 2-injection Mechanism, 21-stage, 22-first sliding mechanism, 221-first sliding member, 23-second sliding mechanism, 231-second sliding member, 3-rack, 31-sample placement position, 4-image acquisition device, 5-light source assembly, 6-shading part, 61-hood, 62-rack, 63-gear, 64-injection port.

Detailed ways

The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present invention, but not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation or a specific orientation. construction and operation, and therefore should not be construed as limiting the invention. Furthermore, the terms "first", "second", and "third" are used for descriptive purposes only and should not be construed to indicate or imply relative importance.

In the description of the present invention, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense, unless otherwise expressly specified and limited, for example, it may be a fixed connection or a detachable connection connection, or integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal connection of two components, which can be a wireless connection or a wired connection connect. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in specific situations.

In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

Example

1 to 8, the present invention provides a nucleic acid detection device 100, including:

The rack 3 is provided with a sample placement position 31;

The image acquisition device 4 is arranged on the rack 3 and is located above the sample placement position 31;

The light source assembly 5 is arranged on the rack 3 and is located above the sample placement position 31; the light source assembly 5 is used for illuminating a light source only for exciting fluorescence;

The light-shielding member 6 is arranged at least around the light-emitting surface of the light source assembly 5 and the outer circumference of the lens of the image collector 4 , and the bottom of the light-shielding member 6 at least partially extends to the outer circumference of the sample placement position 31 . The setting of this technical solution enables the sample placement position 31 to place a certain number of samples, which is easy to achieve high-throughput sample detection, and can greatly shorten the detection time of conventional nucleic acid detection methods on the premise of ensuring sensitivity, and is suitable for large-scale nucleic acid detection methods. The application scenario of detection; meanwhile, the setting of the light shielding member 6 can reduce the noise of the fluorescent signal, improve the accuracy of the amount of PCR reaction product reflected by the fluorescent signal, and improve the detection accuracy of the nucleic acid detection device 100 .

Specifically, the light-shielding member 6 is a cover body 61 with an opening at the bottom, and the top of the cover body 61 is disposed above the tops of the image collector 4 and the light source assembly 5 , and the light-shielding member 6 can cover the light source assembly. The light emitted by 5 and the image collector 4 are shielded to reduce the noise of the fluorescent signal.

As shown in FIG. 4 , the light-shielding member 6 is vertically slidably arranged on the frame 3 through a lifting mechanism, so that when a sample box 1 needs to be detected, a part of the light-shielding member 6 can slide upward to facilitate the sample box. 1 to enter.

Further, the lifting mechanism includes a lifting member fixed on the shading member 6, and a first driving component that drives the lifting member to perform a lifting motion and is arranged on the frame 3, so that the shading member 6 can be relatively Rack 3 moves automatically for easy control.

In an embodiment of the present invention, the lifting component is a rack 62 arranged on one side of the light shielding component 6 along the vertical direction, and the first driving component is fixed on the frame 3 and is connected to the frame 3 . The gear 63 meshed with the rack 62 and the drive motor electrically connected to the gear 63 to drive the gear 63 to rotate will drive the rack 62 by driving the gear 63 to rotate, and also drive the shading member 6 to face each other. The gear 63 moves up and down, so as to realize the automatic movement of the shading member 6 .

Of course, in other embodiments, the lifting mechanism is a lifting cylinder fixed on the frame 3 , and the shading member 6 is fixedly connected to the output end of the lifting cylinder to realize the automatic movement of the shading member 6 . As long as the automatic movement of the shading member 6 can be realized, the object of the present invention can be achieved.

Preferably, at least one light absorbing layer is provided on the outer wall surface and/or the inner wall surface of the light shielding member 6 . Specifically, the light-absorbing layer is made of aluminum black oxide, so that the light-absorbing layer can absorb light and prevent the reflection of light, and can obtain lower background noise during fluorescence signal detection, reduce the noise of fluorescent signal, and improve nucleic acid. The detection accuracy of the detection device 100 .

In this embodiment, as shown in FIGS. 6 to 8 , the nucleic acid detection device 100 further includes a sample box 1 disposed on the sample placement position 31 ; the sample is disposed in the sample box 1 by being disposed in the sample box 1 The sample placement position 31; the top of the sample box 1 is provided with a detection port 11 corresponding to the samples in it; When the sample box 1 is being detected, the light-shielding member 6 can shield its outer periphery, further shielding the external stray light, lower background noise can be obtained when the fluorescent signal is detected, the noise of the fluorescent signal can be reduced, and the performance of the nucleic acid detection device 100 can be improved. Detection accuracy.

The sample box 1 includes: a lower cover 13, which has a accommodating cavity; an upper cover 12, which is movably arranged on the top opening of the lower cover 13 driven by a first driver, and the upper cover 12 is buckled. When being closed on the lower cover 13 , a accommodating cavity is enclosed with the lower cover 13 ; the upper cover 12 is provided with the detection port 11 . Specifically, the upper cover 12 can be flipped and connected to the top side of the lower cover 13 , or the upper cover 12 can be slid back and forth on the top of the lower cover 13 , as long as the upper cover 12 is reached The purpose of the present invention can be achieved by being movably connected to the lower cover 13 , and the arrangement of the above technical solutions facilitates the placement and detection of the sample box 1 , and also facilitates the control of the opening and closing of the upper cover 12 .

6 and 7, in an embodiment of the present invention, the sample box 1 is provided with a rotating motor 111 respectively connected to the upper cover 12 and the lower cover 13 to drive the upper cover 12 and the lower cover 13. The opening and closing of the lower cover plate 13 has a high overall degree of automation, and can well protect the detection sample.

6 and 8, in another embodiment of the present invention, the sample box 1 further has a reducer 112 connected to the output end of the rotating motor 111, and the output end of the reducer 112 is equipped with a belt. Wheel 113. Through the arrangement of the above technical solutions, the service life of the rotating motor 111 can be prolonged, so that the opening and closing of the upper cover plate 12 and the lower cover plate 13 are more stable.

Specifically, at least one heating element is respectively provided on the inner wall surfaces of the upper cover plate 12 and the lower cover plate 13; a temperature detector and a controller electrically connected to both the temperature detector and the heating element; and/or the At least one layer of heat-resistant coating is provided on the outer walls of the upper cover 12 and/or the lower cover 13 . By sensing the temperature of the upper cover plate 12 and the lower cover plate 13 by the corresponding temperature detectors, the controller controls the corresponding heating elements to heat, thereby avoiding the phenomenon of sample condensation, thereby ensuring the accuracy of the detection results. The heat-resistant coating is a black high polymer, such as black polyoxymethylene (POM), black nylon material, etc., which can not only prevent the side of the sample box 1 facing away from the PCR board from being overheated and scalding the staff, but also can prevent the black coating No reflection is generated when the light source assembly 5 is irradiated, lower background noise can be obtained when the fluorescent signal is detected, the noise of the fluorescent signal is reduced, and the detection accuracy of the nucleic acid detection device 100 is improved.

Further, at least one heat-conducting plate is also provided on the side surface of the heating element facing away from the cover plate where it is located; at least one placing groove is set in the accommodating cavity of the lower cover plate 13, and the placing groove is For the PCR board to be embedded, the setting of the heat-conducting plate is further conducive to heat conduction. Preferably, the heat-conducting plate is an aluminum plate; the placement groove is convenient for the PCR board to be embedded, thereby facilitating its alignment detection.

As shown in FIG. 1 to FIG. 5 , the shading member 6 is sleeved on the outer periphery of the rack 3 ; the side wall of the shading member 6 is provided with a sample inlet 64 that communicates with the sample placement position 31 . ;

The nucleic acid detection device 100 further includes a sample injection mechanism 2, and the sample box 1 is provided on the sample injection mechanism 2 and is driven by the sample injection mechanism 2 to slide in a first direction and pass through the injection port 64. Protruding into or out of the sample placement position 31; and sliding along the second direction perpendicular to the first direction at the sample placement position 31, the shading member 6 can shield most of the stray light, and at the same time can realize the sample box 1 overall injection, and also facilitates the alignment of the PCR plate during detection.

The sample feeding mechanism 2 includes: a first sliding mechanism 22, which is arranged on the frame 3 and performs reciprocating linear sliding along the first direction; a second sliding mechanism 23, which is arranged on the first sliding mechanism 22 and moves along the second direction. Do reciprocating direct sliding; the stage 21 is arranged on the second sliding member 231 of the second sliding mechanism 23 ; the sample box 1 is installed on the top surface of the stage 21 to realize the sampling process of the sample box 1 , to ensure the stability of the sampling process, and to further ensure the quality of the samples and test results.

Specifically, in one embodiment, the first sliding mechanism 22 and the second sliding mechanism 23 both include a rotary motor and a lead screw connected to the output end of the rotary motor, and the first sliding mechanism 22 has a first sliding mechanism that slides in a first direction. The sliding member 221, the second sliding mechanism 23 is fixed on the first sliding member 221, the second sliding member 231 slides along the second direction and carries the stage 21 to move along the second direction, so as to realize the sample injection process of the sample box 1 , and the injection process is stable.

Of course, in other embodiments, the first sliding mechanism 22 and the second sliding mechanism 23 may be configured as telescopic cylinders, and the first sliding mechanism 22 has a first sliding member 221 disposed at the output end of the corresponding telescopic cylinder to move along the Sliding in one direction, the second sliding mechanism 23 is fixed on the first sliding member 221, and the second sliding mechanism 23 has a second sliding member 231 disposed at the output end of the corresponding telescopic cylinder to slide in the second direction and carry the load The stage 21 moves along the second direction, so that the sample injection process of the sample box 1 is realized, and the sample injection process is stable. As long as the moving mechanism for the stage 21 can be realized, the object of the present invention can be achieved.

The detection method of the accounting detection device of the present invention includes:

Control the upper cover to open, and place the PCR plate correspondingly in the placement groove of the lower cover;

Control the rotating motor to cover the upper cover plate, and control the heating element to heat the upper cover plate and the lower cover plate, and control the heating element to heat the upper cover plate and the lower cover plate to the preset temperature according to the temperature of the temperature detector;

Control the operation of the lifting mechanism so that the shading component shields the light source assembly and the image collector;

The sample feeding mechanism is controlled to carry the sample box into the opening on the front side of the light-shielding cover, and the lifting mechanism and work are controlled so that the light-shielding component shields the sample box for fluorescence signal detection.

To sum up, the present invention provides a nucleic acid detection device 100 , comprising: a rack 3 , which is provided with a sample placement position 31 ; an image collector 4 , which is arranged on the rack 3 and located above the sample placement position 31 ; The light source assembly 5 is arranged on the rack 3 and is located above the sample placement position 31; the light source assembly 5 is used for illuminating a light source only used to excite fluorescence; the light shielding member 6 is at least arranged around the light source assembly 5 and the outer circumference of the lens of the image collector 4 , and the bottom of the light shielding member 6 at least partially extends to the outer circumference of the sample placement position 31 . With the configuration of this technical solution, the sample placement position 31 can place a certain amount of samples. On the premise of ensuring the sensitivity, the detection time of the conventional nucleic acid detection method can be greatly shortened, and it is suitable for the application scene of large-scale nucleic acid detection; The setting can reduce the noise of the fluorescent signal, improve the accuracy of the amount of PCR reaction product reflected by the fluorescent signal, and improve the detection accuracy of the nucleic acid detection device 100 .

Obviously, the above-mentioned embodiments are only examples for clear description, and are not intended to limit the implementation manner. For those of ordinary skill in the art, changes or modifications in other different forms can also be made on the basis of the above description. There is no need and cannot be exhaustive of all implementations here. And the obvious changes or changes derived from this are still within the protection scope of the present invention.

Claims (11)

1. A nucleic acid detecting apparatus, comprising:

a rack provided with a sample placing position;

the image collector is arranged on the rack and is positioned above the sample placing position;

the light source assembly is arranged on the rack and is positioned above the sample placing position; the light source assembly is used for irradiating a light source only used for exciting fluorescence;

the light shielding part at least winds the periphery of the light emitting surface of the light source component and the periphery of the lens of the image collector by one circle, and the bottom of the light shielding part at least partially extends to the periphery of the sample placing position.

2. The nucleic acid detecting device according to claim 1, wherein the light shielding member is a cover body having an opening at a bottom thereof, and a top of the cover body covers over the top of the image collector and the light source assembly.

3. The nucleic acid detecting apparatus according to claim 1, wherein the light blocking member is slidably provided on the frame in a vertical direction by an elevating mechanism.

4. The nucleic acid detecting apparatus according to claim 3, wherein the elevating mechanism includes an elevating member fixed to the light shielding member, and a first driving unit provided on the frame for driving the elevating member to move up and down.

5. The nucleic acid detecting device according to any one of claims 1 to 4, wherein at least one light absorbing layer is provided on an outer wall surface and/or an inner wall surface of the light shielding member.

6. The nucleic acid detecting apparatus according to claim 1, further comprising a sample cartridge provided at the sample placement site;

a sample is arranged on the sample placement site by being arranged in the sample cartridge; the top of the sample box is provided with detection ports which are in one-to-one correspondence with samples in the sample box;

the bottom of the light shielding part is at least partially wound on the periphery of the sample box.

7. The nucleic acid detecting apparatus according to claim 6, wherein the sample cartridge includes a lower cover plate having a housing chamber;

the upper cover plate is movably arranged on the top opening of the lower cover plate under the driving of the first driver, and when the upper cover plate is buckled on the lower cover plate, an accommodating cavity is formed by the upper cover plate and the lower cover plate; the upper cover plate is provided with the detection port.

8. The nucleic acid detecting apparatus according to claim 7, wherein at least one heating element is provided on an inner wall surface of each of the upper cover plate and the lower cover plate; the temperature detector and the controller are electrically connected with the temperature detector and the heating element; and/or

And at least one layer of heat-resistant coating is arranged on the outer wall surface of the upper cover plate and/or the lower cover plate.

9. The nucleic acid detecting device according to claim 8, wherein at least one heat conducting plate is further provided on a surface of the heating element facing away from the cover plate on which each heating element is provided; or

At least one placing groove is formed in the containing cavity of the lower cover plate, and the placing groove is used for embedding the PCR plate.

10. The nucleic acid detecting device according to any one of claims 6 to 9, wherein the light shielding member is fitted over an outer periphery of the housing; a sample inlet communicated with the sample placing position is formed in the side wall of the shading part;

the nucleic acid detection device also comprises a sample feeding mechanism, and the sample box is arranged on the sample feeding mechanism and driven by the sample feeding mechanism to slide along a first direction to extend into or withdraw from the sample placing position through the sample feeding port; and sliding at the sample placement location in a second direction perpendicular to the first direction.

11. The nucleic acid detecting device according to claim 10, wherein the sample introduction mechanism includes:

the first sliding mechanism is arranged on the rack and linearly slides in a reciprocating manner along a first direction;

the second sliding mechanism is arranged on the first sliding mechanism and directly slides in a reciprocating manner along a second direction;

a carrier arranged on a second slider of the second sliding mechanism; the sample cartridge is mounted on a top surface of the stage.

Images