CN111974475B - Blood component separator - Google Patents
Blood component separator Download PDFInfo
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- CN111974475B CN111974475B CN202010843167.XA CN202010843167A CN111974475B CN 111974475 B CN111974475 B CN 111974475B CN 202010843167 A CN202010843167 A CN 202010843167A CN 111974475 B CN111974475 B CN 111974475B
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- 210000004369 blood Anatomy 0.000 claims description 32
- 239000008280 blood Substances 0.000 claims description 32
- 238000007789 sealing Methods 0.000 claims description 20
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- 238000000926 separation method Methods 0.000 abstract description 29
- 210000002381 plasma Anatomy 0.000 description 14
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B7/00—Elements of centrifuges
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Centrifugal Separators (AREA)
- External Artificial Organs (AREA)
Abstract
本发明公开了血液成分分离器，一次离心管内设有隔板、将一次离心管沿轴向分为上部腔室I和下部腔室II；通过调节件调节腔室II的容积大小；隔板上表面同轴设有圆环形的环槽；环槽内沿环向间隔设有盲孔和贯穿隔板的通孔；盲孔内底部设有橡胶密封垫；活塞与二次离心管配合；二次离心管的封闭端端面设有安装孔；针管的一端口外壁沿周向环设有弹性橡胶圈，弹性橡胶圈的外缘下表面与二次离心管的封闭端内表面密封固定连接，针管的另一端贯穿安装孔；二次离心管的封闭端由一次离心管的上端敞口伸入腔室I内，且二次离心管与一次离心管周向转动连接。本发明通过一个分离容器就可实现二次离心分离，在分离容器内部实现血液成分转移，无需更换分离器。
The invention discloses a blood component separator. A separator is arranged in a primary centrifuge tube, and the primary centrifuge tube is axially divided into an upper chamber I and a lower chamber II; the volume of the chamber II is adjusted by an adjusting member; The surface is coaxially provided with a circular ring groove; the ring groove is provided with blind holes and through holes penetrating the baffle at intervals along the circumferential direction; the inner bottom of the blind hole is provided with a rubber gasket; the piston is matched with the secondary centrifuge tube; two The closed end face of the secondary centrifuge tube is provided with a mounting hole; the outer wall of one port of the needle tube is provided with an elastic rubber ring along the circumferential ring, and the lower surface of the outer edge of the elastic rubber ring is sealed and fixedly connected with the inner surface of the closed end of the secondary centrifuge tube. One end runs through the mounting hole; the closed end of the secondary centrifuge tube extends into the chamber I by the upper end opening of the primary centrifuge tube, and the secondary centrifuge tube is connected to the primary centrifuge tube by circumferential rotation. The invention can realize secondary centrifugal separation through one separation container, and realize the transfer of blood components inside the separation container without replacing the separator.
Description
Technical Field
The invention relates to a medical instrument, in particular to a blood component separator.
Background
Platelet-rich plasma (PRP), which is Platelet concentrate obtained by centrifugation of autologous whole blood, is used as a Platelet-rich plasma. PRP contains a large amount of growth factors and proteins and has high medical value, and platelet-rich plasma is plasma which is obtained by centrifuging whole blood of animals or humans and is rich in platelets at high concentration, and becomes a jelly after thrombin is added thereto, and thus is also called platelet-rich gel or platelet-rich leukocyte gel (PLG). PRP contains a large number of growth factors such as platelet-derived growth factor (PDGF), transforming growth factor beta (TGF- β), insulin-like growth factor 1(IGF-1), and the like.
In the prior art, in order to obtain platelet-rich plasma, whole blood is first placed in a centrifuge tube for centrifugal separation, so that the blood is basically divided into three layers: a blood cell layer, a leucocyte layer and a plasma layer; then, the upper plasma layer and the middle white membrane layer are taken out and transferred to another centrifuge tube for secondary centrifugal separation, and finally platelet-rich plasma is obtained. However, the separation method using the conventional blood separation apparatus has problems that the blood is easily contaminated and the number of processes is increased in the process of moving the blood to a different centrifugal separation container in the centrifugal process, and it is difficult to concentrate the platelets to a high concentration using the conventional blood separation apparatus.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: in the separation method using the existing blood separation device, the blood is easy to be polluted in the process of moving the blood to different centrifugal separation containers. The present invention provides a blood component separator that solves the above problems.
The invention is realized by the following technical scheme:
the blood component separator comprises a primary centrifugal tube, a secondary centrifugal tube and a piston; the axial upper end of the primary centrifugal tube is open; a partition plate is arranged in the primary centrifugal tube and divides the primary centrifugal tube into an upper cavity I and a lower cavity II along the axial direction; an adjusting piece is arranged at the axial bottom end of the primary centrifugal tube and used for adjusting the volume of the cavity II; the upper surface of the partition plate is provided with an annular ring groove, and the axis of the ring groove is superposed with the axis of the primary centrifugal tube; blind holes and through holes penetrating through the partition plate are arranged on the inner bottom surface of the annular groove at intervals along the annular direction; a rubber sealing gasket is arranged at the bottom in the blind hole; the axial bottom end of the secondary centrifugal tube is closed, the axial top end of the secondary centrifugal tube is open, one end of the piston extends into the secondary centrifugal tube from the open top end of the secondary centrifugal tube, and the piston is in linear reciprocating dynamic sealing fit with the secondary centrifugal tube; the end face of the closed end of the secondary centrifugal tube is provided with a mounting hole in a penetrating manner, the secondary centrifugal tube further comprises a needle tube, the outer wall of one port of the needle tube is circumferentially provided with an elastic rubber ring, the lower surface of the outer edge of the elastic rubber ring is fixedly connected with the inner surface of the closed end of the secondary centrifugal tube in a sealing manner, and the other end of the needle tube penetrates through the mounting hole; the closed end of the secondary centrifugal tube extends into the chamber I from the upper end opening of the primary centrifugal tube, the secondary centrifugal tube is connected with the primary centrifugal tube in a circumferential rotating mode, and the rotating axes of the secondary centrifugal tube and the primary centrifugal tube are superposed with the axis of the primary centrifugal tube and the axis of the secondary centrifugal tube; in the process of transferring part of blood components in the cavity II to the cavity I, the needle tube penetrates through the through hole of the partition plate and then extends into the cavity II, and part of the blood components in the cavity II are transferred to the cavity I through the action of the piston; in the process of collecting partial blood components in the chamber I, the secondary centrifugal tube is rotated, the needle tube slides along the annular groove until entering the blind hole, and the bottom end of the needle tube is sealed by a rubber sealing gasket.
The separation method using the conventional blood separation apparatus has problems in that the blood is easily contaminated and the number of processes is increased in the process of moving the blood to a different centrifugal separation vessel in the centrifugal process, and it is difficult to concentrate the platelets to a high concentration using the conventional blood separation apparatus. Based on this technical background, the present invention provides a blood component separator that can achieve secondary centrifugal separation by a single separation container, and that can achieve blood component transfer within the separation container without replacing the separator.
The working principle of the invention is as follows:
1. inject the whole blood of gathering into cavity II earlier, the adjustment regulating part, suitably increase cavity II's volume makes the whole blood of pouring into all be located the cavity II of baffle below in, then carries out once centrifugal separation and makes the preliminary layering of whole blood, mainly divide into the three-layer: lower red blood cell layer, middle leucocyte layer and upper plasma layer.
2. In transferring the upper plasma and middle buffy coat layers in chamber II to chamber I: the needle tube penetrates through the through hole in the partition plate, extends into the cavity II and is immersed into the upper plasma, the air pressure in the cavity I is smaller than the air pressure in the cavity II by pulling the piston outwards, the upper plasma in the cavity II is sucked into the cavity I through the needle tube, and finally the upper plasma and the middle white membranous layer in the cavity II are all transferred into the cavity I and then the piston is stopped to be pulled; in the process, the adjusting piece needs to be continuously adjusted, the solvent in the cavity II is gradually reduced, and the lower port of the needle tube can be always immersed into the upper liquid level in the cavity II.
3. Part of the blood component is collected in chamber I: after the operation of the step 2 is finished, the secondary centrifugal tube of the secondary centrifugal tube rotates in the circumferential direction relative to the primary centrifugal tube to drive the needle tube to synchronously move, the needle tube rotates, the acting force and the through hole port are blocked and is jacked upwards, the elastic rubber ring at the upper port of the needle tube is stretched and deformed until the lower port of the needle tube is jacked to the inner bottom surface of the annular groove, then the needle tube slides along the circular track of the annular groove under the rotating action until the needle tube reaches the blind hole, the elastic rubber ring is stretched and deformed to be reduced slowly, and the bottom port of the needle tube is contacted with the rubber sealing gasket in the blind hole; at the moment, the position of the lower port of the needle tube is raised relative to the needle tube at the position of the through hole, the elastic rubber ring is still in a stretched deformation state, the needle tube is pressed down by the restoring acting force of the elastic rubber ring, the bottom port of the needle tube is in pressing contact with the rubber sealing gasket, the sealing effect is achieved, and the blood component entering the cavity I is prevented from being guided back to the cavity II. Then, the second centrifugation is performed to obtain platelet-rich plasma.
Preferably, the top surface of the partition board on the side of the chamber I is of a plane structure, and the bottom surface of the partition board on the side of the chamber II is of an inclined plane structure; and the through hole penetrates through the partition plate from the position with the minimum thickness of the partition plate.
According to the invention, the partition board is arranged to be in an inclined plane structure, so that on one hand, the blind holes are ensured not to penetrate through the partition board; on the other hand, the caliber of the position close to the clapboard in the cavity II is favorably reduced, and the needle tube can conveniently absorb the upper blood of the cavity II through the through hole.
Preferably, the bottom surfaces of the ring grooves are sequentially and continuously arranged obliquely on the ring-shaped track from the through hole to the blind hole; and the height of the bottom surface of the ring groove where the blind hole is located is greater than that of the bottom surface of the ring groove where the through hole is located.
The bottom surface of the ring groove is sequentially and continuously inclined, so that the blood remained in the ring groove can be favorably refluxed into the chamber II
Preferably, the through holes and the blind holes are located at two radial ends of the ring groove, and the center of the ring groove is used as a base point, and the number of the through holes and the number of the blind holes are 180 degrees.
With the maximum interval setting of through-hole and blind hole, conveniently make the inclined plane slope of through-hole way blind hole lower, the guarantee slip process goes on gently to have the steady tensile deformation effort of exerting of transition to the elastic rubber circle.
Preferably, the pipe section of the needle tube extending into the through hole is of an inverted cone structure; and rounding structures are arranged on the circumferential surfaces of the top ports of the through hole and the blind hole.
Design like this, at the rotation secondary centrifuging tube in-process, do benefit to needle tubing and through-hole port department smooth contact.
Preferably, the outer wall of the upper end of the primary centrifugal tube is circumferentially provided with an annular baffle I, and the side wall of the secondary centrifugal tube is circumferentially provided with an annular baffle II; still including rotating the ring cover, annular spacing groove has been seted up along circumference to the inside wall that rotates the ring cover, annular baffle I and annular baffle II embedding annular spacing inslot realize rotating the mutual axial displacement of ring cover restriction primary centrifuge tube and secondary centrifuge tube.
Set up the aim at that rotates sleeve pipe, ring baffle I and ring baffle II, when the mutual circumferential direction of guarantee primary centrifuge tube and secondary centrifuge tube, restriction both take place axial displacement each other.
Preferably, the outer surface of the bottom end surface of the secondary centrifugal tube is provided with a rotating shaft; the center of a circle of the upper surface of the partition plate is provided with a rotating shaft mounting hole, and the rotating shaft is embedded into the rotating shaft mounting hole and is connected in a rotating mode.
Through pivot and pivot mounting hole adaptation, still can play radial limiting displacement between secondary centrifuging tube and the primary centrifuge tube.
Preferably, the side walls of the primary centrifugal tube and the secondary centrifugal tube are provided with air pressure balance holes; and a filtering unit is arranged in the pore passage of the air pressure balancing hole.
Further preferably, the side walls of the primary centrifugal tube and the secondary centrifugal tube are provided with blood injection and blood component output pore channels.
Preferably, the adjusting part is a sleeve, one axial end of the sleeve is closed, and the other axial end of the sleeve is open; the sleeve is sleeved at the lower end of the primary centrifugal tube from the open end, and the inner wall of the sleeve is in threaded sealing connection with the outer wall of the primary centrifugal tube.
The invention has the following advantages and beneficial effects:
the invention provides a blood component separator, which can realize secondary centrifugal separation through a separation container, realize blood component transfer in the separation container and does not need to replace the separator.
Drawings
The accompanying drawings, which are included to provide a further understanding of the embodiments of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the principles of the invention. In the drawings:
FIG. 1 is a front view of a blood component separator of the present invention;
FIG. 2 is a schematic axial cross-sectional view of the structure of FIG. 1;
FIG. 3 is a front view of the separator plate of the present invention;
FIG. 4 is a top view of a separator plate of the present invention;
FIG. 5 is a schematic view of the blood component separator of the present invention in a blood transfer state;
fig. 6 is a schematic view showing the structure of the blood component separator of the present invention after completion of blood transfer.
Reference numbers and corresponding part names in the drawings: 1-primary centrifugal tube, 2-secondary centrifugal tube, 3-piston, 4-partition plate, 5-ring groove, 6-blind hole, 7-through hole, 8-rubber sealing gasket, 9-needle tube, 10-elastic rubber ring, 11-annular baffle I, 12-annular baffle II, 13-rotating ring sleeve, 14-rotating shaft, 15-rotating shaft mounting hole and 16-sleeve.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.
Example 1
This embodiment provides a blood component separator, including primary centrifuge tube 1, still include secondary centrifuge tube 2 and piston 3, primary centrifuge tube 1 and secondary centrifuge tube all are the pipe structure, and piston 3 is cylindricly. The axial upper end of the primary centrifugal tube 1 is open; a partition plate 4 is arranged in the primary centrifugal tube 1, the radial direction of the partition plate 4 is vertical to the axial direction of the primary centrifugal tube 1, and the partition plate 4 divides the primary centrifugal tube 1 into an upper cavity I and a lower cavity II along the axial direction; the axial bottom of primary centrifuge tube 1 is equipped with the regulating part, and the regulating part is used for adjusting chamber II's volume size. The upper surface of the partition plate 4 is provided with an annular ring groove 5, the ring groove 5 does not penetrate through the partition plate 4, and the axis of the ring groove 5 is superposed with the axis of the primary centrifugal tube 1; blind holes 6 and through holes 7 penetrating through the partition plate 4 are arranged on the inner bottom surface of the annular groove 5 at intervals along the annular direction; the bottom in the blind hole 6 is provided with a rubber sealing gasket 8; in the axial direction of the primary centrifugal tube 1, the height of the inner bottom surface of the blind hole 6 is flush with that of the bottom end surface of the through hole 7, or the height of the inner bottom surface of the blind hole 6 is greater than that of the bottom end surface of the through hole 7.
The axial bottom of secondary centrifuge tube 2 is sealed, the axial top is uncovered, and the one end of piston 3 is stretched into secondary centrifuge tube 2 by the open end in top of secondary centrifuge tube 2, and the reciprocal movive seal cooperation of straight line between piston 3 and the secondary centrifuge tube 2. The end face of the closed end of the secondary centrifugal tube 2 is provided with a mounting hole in a penetrating way; the device is characterized by further comprising a needle tube 9, wherein an elastic rubber ring 10 is arranged on the outer wall of one port of the needle tube 9 in a circumferential ring mode, the lower surface of the outer edge of the elastic rubber ring 10 is fixedly connected with the inner surface of the closed end of the secondary centrifugal tube 2 in a sealing mode, and the other end of the needle tube 9 penetrates through the mounting hole; the blind end of secondary centrifuge tube 2 is opened by the upper end of primary centrifuge tube 1 and is stretched into in cavity I, and secondary centrifuge tube 2 is connected with 1 circumferential direction of primary centrifuge tube, and axis of rotation between them and the axis of primary centrifuge tube 1 and the axis coincidence of secondary centrifuge tube 2.
The working principle of the invention is as follows:
(1) inject the whole blood of gathering into cavity II earlier, the adjustment regulating part, suitably increase cavity II's volume makes the whole blood of pouring into all be located the cavity II of baffle 4 below in, then carries out once centrifugal separation and makes the preliminary layering of whole blood, mainly divide into the three-layer: lower red blood cell layer, middle leucocyte layer and upper plasma layer.
(2) In transferring the upper plasma and middle buffy coat layers in chamber II to chamber I: after penetrating through the through hole 7 on the partition board 4, the needle tube 9 extends into the chamber II and is immersed into the upper plasma, the piston 3 is pulled outwards to act, so that the air pressure in the chamber I is smaller than that in the chamber II, the upper plasma in the chamber II is sucked into the chamber I through the needle tube 9, and finally the upper plasma and the middle white membranous layer in the chamber II are completely transferred into the chamber I, and then the piston 3 is stopped being pulled; in the process, the adjusting piece needs to be continuously adjusted, the solvent in the cavity II is gradually reduced, and the lower port of the needle tube 9 can be always immersed into the upper liquid level in the cavity II.
(3) Part of the blood component is collected in chamber I: after the operation of the step (2) is finished, the secondary centrifugal tube 2 is rotated to perform circumferential rotation action relative to the primary centrifugal tube 1, the needle tube 9 is driven to perform synchronous action, the needle tube 9 is rotated to apply force and the port of the through hole 7 is blocked and is jacked upwards, the elastic rubber ring 10 at the upper port of the needle tube 9 is stretched and deformed until the lower port of the needle tube 9 is jacked to the inner bottom surface of the annular groove 5, then the needle tube 9 slides along the circular track of the annular groove 5 under the rotation action until the needle tube reaches the blind hole 6, the elastic rubber ring 10 is stretched and deformed to be gradually reduced, and the bottom port of the needle tube 9 is contacted with the rubber sealing gasket 8 in the blind hole 6; at the moment, the position of the lower port of the needle tube 9 is raised relative to the position of the needle tube 9 at the through hole 7, the elastic rubber ring 10 is still in a stretched and deformed state, the needle tube 9 is pressed down by the restoring acting force of the elastic rubber ring 9, the bottom port of the needle tube 9 can be in pressing contact with the rubber sealing gasket 8, the sealing effect is achieved, and the blood component entering the cavity I is prevented from being guided back to the cavity II. Then, the second centrifugation is performed to obtain platelet-rich plasma.
Wherein, when the needle tubing 9 is slided to the blind hole 6 along the spout 5 by the through-hole 7, because the annular groove 5 is continuous ring structure, consequently can all realize above-mentioned operation by arbitrary forward or reverse rotation secondary centrifuge tube 2.
Example 2
The structure is further improved on the basis of the embodiment 1, the top surface of the partition plate 4 on the side of the chamber I is of a plane structure, and the bottom surface of the partition plate 4 on the side of the chamber II is of an inclined plane structure; the through hole 7 penetrates through the partition plate 4 from the position with the minimum thickness of the partition plate 4, and the partition plate 4 is arranged to be in an inclined plane structure, so that on one hand, the blind hole 6 is guaranteed not to penetrate through the partition plate 4; on the other hand, the caliber of the position close to the clapboard 4 in the cavity II is favorably reduced, and the needle tube 4 can conveniently absorb the upper-layer blood of the cavity II through the through hole 7.
On the circular track from the through hole 7 to the blind hole 6, the bottom surfaces of the ring grooves 5 are sequentially and continuously arranged in an inclined manner; and the bottom surface of the ring groove 5 where the blind hole 6 is located is higher than the bottom surface of the ring groove 5 where the through hole 7 is located, and the bottom surfaces of the ring grooves 5 are sequentially and continuously inclined, so that the blood remained in the ring grooves 5 can flow back to the chamber II.
The through hole 7 and the blind hole 6 are positioned at two radial ends of the ring groove 5, and the through hole 7 and the blind hole 6 are 180 degrees by taking the circle center of the ring groove 5 as a base point. Set up through-hole 7 and the 6 biggest interval of blind hole, conveniently make through-hole 7 lower to the inclined plane slope of blind hole 6, the guarantee slip process goes on gently to and have the steady tensile deformation effort of exerting of transition to elastic rubber circle 10.
The pipe section of the needle tube 9 extending into the through hole 7 is in an inverted cone structure; the circumferential surfaces of the top ports of the through hole 7 and the blind hole 6 are both provided with a rounding structure, so that the needle tube 9 is in smooth contact with the port of the through hole 7.
Example 3
The device is further improved on the basis of the embodiment 2, the outer wall of the upper end of the primary centrifugal tube 1 is circumferentially provided with an annular baffle I11, and the side wall of the secondary centrifugal tube 2 is circumferentially provided with an annular baffle II 12; still including rotating ring cover 13, annular spacing groove has been seted up along circumference to the inside wall that rotates ring cover 13, annular baffle I11 and annular baffle II12 embedding annular spacing inslot realize rotating ring cover 13 restriction one-time centrifuging tube 1 and the mutual axial displacement of secondary centrifuging tube 2. Set up the aim at that rotates sleeve pipe 13, ring baffle I11 and ring baffle II12, when the mutual circumferential direction of guarantee centrifugal tube 1 and secondary centrifuge tube 2, restriction both take place axial displacement each other.
The outer surface of the bottom end surface of the secondary centrifugal tube 2 is provided with a rotating shaft 14; the center of the circle of the upper surface of the partition board 4 is provided with a rotating shaft mounting hole 15, and the rotating shaft 14 is embedded into the rotating shaft mounting hole 15 and is connected in a rotating mode. Through the adaptation of the rotating shaft 14 and the rotating shaft mounting hole 15, the radial limiting effect can be achieved between the secondary centrifugal tube 2 and the primary centrifugal tube 1.
Example 4
The further improvement is based on the embodiment 3, the side walls of the primary centrifugal tube 1 and the secondary centrifugal tube 2 are both provided with air pressure balance holes (not shown in the figure); a filtering unit is arranged in a pore passage of the air pressure balancing hole; the side walls of the primary centrifugal tube 1 and the secondary centrifugal tube 2 are provided with blood injection and blood component output channels (not shown).
Example 5
The separator is further improved on the basis of the separator in any one of the embodiments 1 to 4, the adjusting piece adopts a sleeve 16, one axial end of the sleeve 16 is closed, and the other axial end of the sleeve 16 is open; the sleeve 16 is sleeved at the lower end of the primary centrifugal tube 1 from the open end, and the inner wall of the sleeve 16 is in threaded sealing connection with the outer wall of the primary centrifugal tube 1.
In addition, in the process of secondary centrifugal separation, the existing blood separation gel can be injected into the chamber I, and the density of the blood separation gel is greater than that of platelet-rich plasma, so that the bottom layer, close to the partition plate 4, in the chamber I after separation is a separation gel layer, and the plasma at each corner of the bottom layer of the chamber I can be effectively separated.
The above-mentioned embodiments are intended to illustrate the objects, technical solutions and advantages of the present invention in further detail, and it should be understood that the above-mentioned embodiments are merely exemplary embodiments of the present invention, and are not intended to limit the scope of the present invention, and any modifications, equivalent substitutions, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. The blood component separator comprises a primary centrifugal tube (1) and is characterized by also comprising a secondary centrifugal tube (2) and a piston (3);
the axial upper end of the primary centrifugal tube (1) is open; a partition plate (4) is arranged in the primary centrifugal tube (1), and the partition plate (4) divides the primary centrifugal tube (1) into an upper cavity I and a lower cavity II along the axial direction; an adjusting piece is arranged at the axial bottom end of the primary centrifugal tube (1) and used for adjusting the volume of the cavity II; the upper surface of the partition plate (4) is provided with an annular ring groove (5), and the axis of the ring groove (5) is superposed with the axis of the primary centrifugal tube (1); blind holes (6) and through holes (7) penetrating through the partition plate (4) are arranged on the inner bottom surface of the annular groove (5) at intervals along the annular direction; a rubber sealing gasket (8) is arranged at the bottom in the blind hole (6);
the axial bottom end of the secondary centrifugal tube (2) is closed, the axial top end of the secondary centrifugal tube is open, one end of the piston (3) extends into the secondary centrifugal tube (2) from the open top end of the secondary centrifugal tube (2), and the piston (3) is in linear reciprocating dynamic sealing fit with the secondary centrifugal tube (2); the end face of the closed end of the secondary centrifugal tube (2) penetrates through a mounting hole, the secondary centrifugal tube further comprises a needle tube (9), an elastic rubber ring (10) is arranged on the outer wall of one port of the needle tube (9) in a circumferential ring mode, the lower surface of the outer edge of the elastic rubber ring (10) is fixedly connected with the inner surface of the closed end of the secondary centrifugal tube (2) in a sealing mode, and the other end of the needle tube (9) penetrates through the mounting hole;
the closed end of the secondary centrifugal tube (2) extends into the chamber I from an opening at the upper end of the primary centrifugal tube (1), the secondary centrifugal tube (2) is connected with the primary centrifugal tube (1) in a circumferential rotating manner, and the rotating axes of the secondary centrifugal tube and the primary centrifugal tube (1) are superposed with the axis of the secondary centrifugal tube (2);
in the process of transferring part of blood components in the cavity II to the cavity I, the needle tube (9) penetrates through the through hole (7) of the partition plate (4) and then extends into the cavity II, and the part of blood components in the cavity II are transferred to the cavity I through the action of the piston (3);
in the process of collecting partial blood components in the cavity I, the secondary centrifugal tube (2) is rotated, the needle tube (9) slides along the annular groove (5) until entering the blind hole (6), and the bottom end of the needle tube (9) is sealed through the rubber sealing gasket (8).
2. A blood component separator according to claim 1, wherein the top surface of the partition plate (4) on the chamber I side is of a planar configuration, and the bottom surface of the partition plate (4) on the chamber II side is of a slanted configuration; and the through hole (7) penetrates through the partition plate (4) from the position with the minimum thickness of the partition plate (4).
3. A blood component separator according to claim 1, wherein the bottom surfaces of the ring grooves (5) are successively inclined in sequence on a circular path from the through hole (7) to the blind hole (6); and the height of the bottom surface of the annular groove (5) where the blind hole (6) is located is greater than that of the bottom surface of the annular groove (5) where the through hole (7) is located.
4. A blood component separator according to claim 3, wherein the through hole (7) and the blind hole (6) are located at both ends of the annular groove (5) in the radial direction, and the through hole (7) and the blind hole (6) are 180 ° from the center of the annular groove (5).
5. A blood component separator according to claim 1, wherein the tube section of the needle tube (9) extending into the through hole (7) has an inverted cone-shaped configuration; and rounding structures are arranged on the circumferential surfaces of the top ports of the through hole (7) and the blind hole (6).
6. The blood component separator according to claim 1, wherein the outer wall of the upper end of the primary centrifugal tube (1) is provided with an annular baffle I (11) along the circumferential direction, and the side wall of the secondary centrifugal tube (2) is provided with an annular baffle II (12) along the circumferential direction; still including rotating ring cover (13), the annular spacing groove has been seted up along circumference to the inside wall that rotates ring cover (13), annular baffle I (11) and annular baffle II (12) embedding annular spacing inslot realize rotating ring cover (13) restriction one-time centrifuging tube (1) and the mutual axial displacement of secondary centrifuging tube (2).
7. A blood component separator according to claim 1, wherein the outer surface of the bottom end face of the secondary centrifuge tube (2) is provided with a rotating shaft (14); the upper surface centre of a circle department of baffle (4) is equipped with pivot mounting hole (15), pivot (14) embedding pivot mounting hole (15) internal rotation is connected.
8. A blood component separator according to claim 1, wherein the side walls of the primary centrifugal tube (1) and the secondary centrifugal tube (2) are provided with air pressure balancing holes; and a filtering unit is arranged in the pore passage of the air pressure balancing hole.
9. A blood component separator according to claim 1, wherein the side walls of the primary centrifuge tube (1) and the secondary centrifuge tube (2) are provided with blood injection and blood component outlet channels.
10. A blood component separator according to claim 1, wherein said regulating member is a sleeve (16), said sleeve (16) being closed at one axial end and open at the other axial end; the sleeve (16) is sleeved at the lower end of the primary centrifugal tube (1) through the open end, and the inner wall of the sleeve (16) is in threaded sealing connection with the outer wall of the primary centrifugal tube (1).
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| CN202010843167.XA CN111974475B (en) | 2020-08-20 | 2020-08-20 | Blood component separator |
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| CN202010843167.XA CN111974475B (en) | 2020-08-20 | 2020-08-20 | Blood component separator |
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| US20100093551A1 (en) * | 2008-10-09 | 2010-04-15 | Decision Biomarkers, Inc. | Liquid Transfer and Filter System |
| CN102458661B (en) * | 2009-05-15 | 2015-11-25 | 贝克顿·迪金森公司 | Density phase separation device |
| CN103619484B (en) * | 2011-05-05 | 2018-03-09 | 干细胞技术公司 | Method and insert for density gradient separation |
| CN103405818B (en) * | 2013-08-28 | 2016-07-06 | 朱悦 | Prepare the devices and methods therefor of Platelet-rich plasm |
| CN105148569B (en) * | 2015-07-02 | 2017-03-01 | 周建明 | A kind of integration autologous platelet rich gel preparation suit |
| EP3383269B1 (en) * | 2015-12-03 | 2020-02-19 | Lacerta Technologies Inc. | Apparatus for preparing blood fraction concentrate |
| CN105596014B (en) * | 2016-01-22 | 2018-05-25 | 黄鹭强 | A kind of double-jacket tube centrifugation injection device |
| CN206853188U (en) * | 2016-12-23 | 2018-01-09 | 宋东范 | A kind of platelet rich plasma separating extraction device |
| US10987672B2 (en) * | 2017-06-08 | 2021-04-27 | Emcyte Corp. | Dual piston centrifuge tube |
| EP3470142A1 (en) * | 2017-10-11 | 2019-04-17 | Orthogen AG | Device comprising a first chamber for receiving a body fluid |
| CN108654141B (en) * | 2018-06-08 | 2020-08-14 | 宁波华科润生物科技有限公司 | A device for preparing platelet-rich plasma |
| CN110856775B (en) * | 2018-08-23 | 2024-09-24 | 康膝生物医疗(深圳)有限公司 | Medical separation and extraction device and method for separating and extracting platelet-rich plasma using the same |
| CN109395434A (en) * | 2018-12-06 | 2019-03-01 | 武汉维斯第医用科技股份有限公司 | Extraction separating tool for platelet rich plasma |
| CN209885297U (en) * | 2019-03-16 | 2020-01-03 | 庄陈 | Platelet-rich plasma separation and extraction kit |
| CN111013197A (en) * | 2019-12-31 | 2020-04-17 | 山东中保康医疗器具有限公司 | Disposable platelet-rich plasma separation device |
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