CN111955538B - Pseudosciaena crocea preservative and using method thereof - Google Patents

Abstract

The invention relates to an antistaling agent for large yellow croaker and a method for using the same. The antistaling agent for large yellow croaker comprises L-phenylalanine and D-phenylalanine. The preservative can be applied on the surface to prolong the shelf life of large yellow croaker at low temperature, and has the effect of inhibiting the growth of Listeria monocytogenes, Shewanella spoilage and Pseudomonas fluorescens among harmful microorganisms in large yellow croaker. , and the generation of volatile base nitrogen during storage and preservation all has a good inhibitory effect, ensuring the freshness of large yellow croaker, and L-phenylalanine and D-phenylalanine in the preservative are amino acids. , is a nutrient that does not harm the human body, nor does it destroy the texture of the food itself.

Description

A kind of antistaling agent for large yellow croaker and using method thereof

technical field

The invention relates to the technical field of fresh-keeping of aquatic products, in particular to a fresh-keeping agent for large yellow croaker and a method for using the same.

Background technique

As a major aquatic country, my country's aquatic product output accounts for more than one third of the global output, ranking first in the world, and the total import and export volume is increasing. Among them, the export species mainly include shrimp, tilapia, large yellow croaker and other varieties. As one of the four traditional marine products in my country, large yellow croaker is mainly distributed in the middle and lower waters from the South China Sea to the east of Leizhou Peninsula, the Taiwan Strait, the East China Sea, and the southern part of the Yellow Sea. It is the main economic fish in my country. With the rapid development of large yellow croaker fishing technology, its consumption has also increased year by year, and it has gradually become one of the fish with the highest yield of a single species in seawater cages in my country.

Large yellow croaker has high protein content, so it is a natural medium for microbial growth. Coupled with the influence of some factors during storage and transportation, there will be some harmful microorganisms on the surface of the fish that are suitable for cold, such as: Listeria monocytogenes, spoilage Shiva Bacteria and Pseudomonas fluorescens breed in large quantities. Shewanella spoilage and Pseudomonas fluorescens will destroy the protein of large yellow croaker and produce a large amount of volatile basic nitrogen and trimethylamine oxide, which greatly reduces the quality of large yellow croaker. Listeria monocytogenes will produce some toxic substances, which will greatly increase the risk of eating large yellow croaker. Therefore, controlling the growth of these microorganisms, inhibiting their production of harmful substances and preventing quality degradation has important social and economic value. Traditional preservatives will increase the acidity of the body and lead to excessive consumption and loss of iodine, iron, calcium and other substances in the human body. Excessive intake may cause gastroenteritis, and may also cause harm to vital organs such as the liver and kidneys. It may also induce symptoms such as allergies. At present, consumers have higher and higher requirements for food safety, and it is easier for consumers to use natural substances to improve the quality and shelf life of food.

SUMMARY OF THE INVENTION

The present invention aims to solve one of the technical problems in the related art at least to a certain extent. For this reason, the object of the present invention is to provide a kind of antistaling agent for large yellow croaker. The preservative can effectively reduce the content of harmful microorganisms in large yellow croaker at low temperature, and significantly improve the quality of large yellow croaker.

To this end, in one aspect of the present invention, the present invention proposes a preservative for large yellow croaker, comprising: L-phenylalanine and D-phenylalanine.

According to the anti-staling agent for large yellow croaker according to the embodiment of the present invention, the actual test results show that the anti-staling agent can extend the shelf life of large yellow croaker in low temperature by smearing on the surface of fish meat, and can prevent Listeria monocytogenes, spoilage, etc. in harmful microorganisms in large yellow croaker. Shewanella, Pseudomonas fluorescens, etc. all have the effect of inhibiting their growth, and the production of volatile base nitrogen during storage and preservation has a good inhibitory effect, ensuring the freshness of large yellow croaker, and the preservation agent The L-phenylalanine and D-phenylalanine in it are amino acids, which are a kind of nutrients, which do not cause harm to the human body, nor damage the texture of the food itself.

In addition, the antistaling agent for large yellow croaker proposed according to the above-mentioned embodiments of the present invention can also have the following additional technical features:

According to an embodiment of the present invention, in terms of mass percentage, the large yellow croaker preservative includes 38-43% of L-phenylalanine and 57-62% of D-phenylalanine.

According to an embodiment of the present invention, in terms of mass percentage, the preservative for large yellow croaker includes 40% of L-phenylalanine and 60% of D-phenylalanine.

According to an embodiment of the present invention, both the L-phenylalanine and the D-phenylalanine are food grade.

In another aspect of the present invention, the present invention proposes a kind of using method of antistaling agent for large yellow croaker, which comprises the following steps:

(1) take by weighing L-phenylalanine and, D-phenylalanine and mix, add water and configure into mixed solution;

(2) After cleaning the fresh large yellow croaker, apply the above mixed solution on the surface of the large yellow croaker, vacuum-pack, and refrigerate at 4°C.

According to the method of using the large yellow croaker preservative according to the embodiment of the present invention, the above-mentioned preservative is smeared on the surface of the large yellow croaker, which can effectively reduce the total number of colonies in the large yellow croaker during storage, reduce the generation of volatile base nitrogen, and slow down spoilage, improve product quality. In addition, the fresh-keeping method has a simple process, does not require fermentation and other technological processes, has relatively low cost, is environmentally friendly, has very broad application prospects, and has great promotion significance.

According to an embodiment of the present invention, the concentration of the mixed solution is 10 mg/mL. Among them, too many amino acids can cause other adverse reactions.

Additional aspects and advantages of the present invention will be set forth, in part, from the following description, and in part will be apparent from the following description, or may be learned by practice of the invention.

Description of drawings

Fig. 1 is the effect that antistaling agent of large yellow croaker inhibits the growth of Shewanella large yellow croaker according to the embodiment of the present invention;

Fig. 2 is the effect that antistaling agent of large yellow croaker suppresses the growth of Pseudomonas large yellow croaker according to the embodiment of the present invention;

Fig. 3 is the effect that large yellow croaker antistaling agent inhibits the growth of large yellow croaker Listeria according to the embodiment of the present invention;

Figure 4 shows the effect of the large yellow croaker preservative on the yield of volatile basic nitrogen in the large yellow croaker according to the embodiment of the present invention.

Detailed ways

The technical solutions of the present invention are described below through specific specific examples. It should be understood that the one or more method steps mentioned in the present invention do not exclude that there are other method steps before and after the combination step or other method steps may be inserted between these explicitly mentioned steps; it should also be understood that these The examples are only used to illustrate the present invention and not to limit the scope of the present invention. Moreover, unless otherwise specified, the numbering of each method step is only a convenient tool for identifying each method step, rather than limiting the arrangement order of each method step or limiting the scope of the present invention. In the case where the technical content is not substantially changed, it should also be regarded as the scope in which the present invention can be implemented.

In order to better understand the above technical solutions, exemplary embodiments of the present invention are described in more detail below. While exemplary embodiments of the present invention have been shown, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that the present invention will be more thoroughly understood, and will fully convey the scope of the present invention to those skilled in the art.

The test materials used in the present invention are all common commercial products and can be purchased in the market.

Instrument: electronic balance.

Reagent: L-phenylalanine, food grade, purchased from Shandong Zhongsen Biotechnology Co., Ltd.;

D-phenylalanine, food grade, was purchased from Shandong Zhongsen Biotechnology Co., Ltd.

The present invention will be described below with reference to specific embodiments. It should be noted that these embodiments are merely illustrative and do not limit the present invention in any way.

Example 1

According to the mass percentage, 40% L-phenylalanine and 60% D-phenylalanine were placed in a dry beaker, stirred with a glass rod for 5 minutes and mixed evenly, and deionized water was added to prepare a concentration of 10 mg/mL mixed solution.

Example 2

According to the mass percentage, 38% L-phenylalanine and 62% D-phenylalanine were placed in a dry beaker, stirred with a glass rod for 5 minutes and mixed well, and deionized water was added to prepare a concentration of 10 mg/mL mixed solution.

Example 3

According to the mass percentage, 41% L-phenylalanine and 59% D-phenylalanine were placed in a dry beaker, stirred with a glass rod for 5 minutes and mixed well, and deionized water was added to prepare a concentration of 10 mg/mL mixed solution.

Example 4

According to the mass percentage, 43% L-phenylalanine and 57% D-phenylalanine were placed in a dry beaker, stirred with a glass rod for 5 minutes and mixed well, and deionized water was added to prepare a concentration of 10 mg/mL mixed solution.

test case

1. Experiment on inhibiting harmful bacteria of large yellow croaker during storage and cold chain transportation

After 24 hours, 48 hours, 72 hours, and 96 hours after smearing the mixed solutions prepared in Examples 1 to 4 on the surface of the large yellow croaker stored at 4°C, the harmful strains of the large yellow croaker were counted (respectively using Iron agar: for enumeration of Shewanella; CFC medium: for enumeration of Pseudomonas; Oxford agar: for enumeration of Listeria). Among them, the control group was smeared with deionized water.

The results are shown in Figure 1 (the effect of anti-staling agent for large yellow croaker on inhibiting the growth of Shewanella large yellow croaker (n=6)), Figure 2 (the effect of anti-staling agent for large yellow croaker on inhibiting the growth of Pseudomonas large yellow croaker (n=6)), Figure 3 (the effect of antistaling agent for large yellow croaker in inhibiting the growth of Listeria large yellow croaker (n=6)). The test results showed that the preservative for large yellow croaker had a significant inhibitory effect on the harmful strains of Shewanella, Pseudomonas and Listeria during low temperature storage, and could prolong the shelf life of large yellow croaker. In addition, compared with Examples 2-4, the preservative for large yellow croaker prepared with the content of L-phenylalanine and D-phenylalanine in Example 1 can make the total number of colonies in the large yellow croaker less.

2. Determination experiment of volatile basic nitrogen content (TVB-N) in large yellow croaker during storage and cold chain transportation

After 24 hours, 48 hours, 72 hours, and 96 hours after the mixed solution prepared in Examples 1 to 4 was evenly spread on the surface of the large yellow croaker stored at 4°C, the content of volatile basic nitrogen in the large yellow croaker was determined. The determination of TVB-N refers to the automatic Kjeldahl method of GB 5009.228-2016, and the unit of data obtained is mg N/100g fish meat. Weigh 10 g of fish meat sample, accurate to 0.001 g, use a tissue homogenizer to crush it, place it in a distillation tube, add 75 mL of ultrapure water, shake to make the sample evenly dispersed in the sample solution, and immerse it for 30 min. Add 1g of MgO, quickly put it into the automatic Kjeldahl nitrogen analyzer, carry out distillation, use 0.01mol/L hydrochloric acid for titration in the program, and calculate according to the following formula.

V2-V1, representing the volume (mL) of hydrochloric acid consumed in the titration;

C, the concentration of hydrochloric acid (mol/L);

V, sample volume (mL);

X, TVB-N content (mg N/100g).

Kjeldahl nitrogen analyzer parameter setting: turn off the functions of automatic waste discharge, automatic alkali addition and automatic water addition, and set the volume of alkali addition and water addition to 0mL. The boric acid receiving solution (20 g/mL) was added and set to 30 mL. Set the distillation time to 180s or the distillation volume to 200mL, whichever comes first. Use the color method to judge the end point, use the mixed indicator solution, add 10 drops of the mixed indicator solution (mixed indicator solution: 1 part of methyl red ethanol solution (1g/mL) and 5 parts of bromocresol green ethanol solution ( 1 g/mL) mixed immediately before use).

The results are shown in Figure 4, during the low-temperature storage process, the volatile base nitrogen content in the large yellow croaker meat of Examples 1 to 4 is equivalent, and all are less than the volatile base nitrogen in the fish meat of the control group, indicating that the large yellow croaker preservative of the present invention is Good preservation effect on protein denaturation. In addition, compared with Examples 2 to 4, the large yellow croaker preservative prepared by the content of L-phenylalanine and D-phenylalanine in Example 1 can make the content of volatile base nitrogen in large yellow croaker meat less. .

To sum up, the large yellow croaker preservative of the present application can effectively reduce the total number of colonies in the large yellow croaker during storage, reduce the generation of volatile base nitrogen, slow down spoilage, and improve product quality; and the amino acid that this large yellow croaker adopts is a nutrient substance , will not destroy the texture of the food itself.

In the description of this specification, description with reference to the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples", etc., mean specific features described in connection with the embodiment or example , structure, material or feature is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms should not be construed as necessarily referring to the same embodiment or example. Furthermore, the particular features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, those skilled in the art may combine and combine the different embodiments or examples described in this specification.

Although the embodiments of the present invention have been shown and described above, it should be understood that the above-mentioned embodiments are exemplary and should not be construed as limiting the present invention. Embodiments are subject to variations, modifications, substitutions and variations.

Claims (2)

1. The use method of the large yellow croaker preservative is characterized by comprising the following steps:

(1) weighing 40% of L-phenylalanine and 60% of D-phenylalanine, uniformly mixing, and adding water to prepare a mixed solution;

(2) cleaning fresh Pseudosciaena crocea, smearing the mixed solution on the surface of Pseudosciaena crocea, vacuum packaging, and refrigerating at 4 deg.C.

2. The use method of the large yellow croaker preservative according to claim 1, wherein the concentration of the mixed solution is 10 mg/mL.

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