CN111943999A - DNA synthesizer - Google Patents
DNA synthesizer Download PDFInfo
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- CN111943999A CN111943999A CN202010759233.5A CN202010759233A CN111943999A CN 111943999 A CN111943999 A CN 111943999A CN 202010759233 A CN202010759233 A CN 202010759233A CN 111943999 A CN111943999 A CN 111943999A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
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Abstract
The invention relates to the field of DNA equipment, and relates to a DNA synthesizer. The DNA synthesizer comprises a synthesizer body, wherein a blowing assembly, a plurality of reagent bottles and a cleaning electromagnetic valve group are arranged on the synthesizer body; inert gas is stored in the gas storage tank; the main air path is connected between the synthesizer body and the air storage tank; the first gas path is connected with the main gas path through a first tee joint and is connected with the synthesizer body to control the ejection of the piston; the second air path is connected with the main air path through a second three-way joint and is connected with an air outlet of a piston on the air blowing assembly; the third gas path is connected with the main gas path through a third three-way joint and is connected with the cleaning solenoid valve group; the fourth gas path is connected with the main gas path through a fourth three-way joint and is connected with the plurality of reagent bottles so as to lead the inert gas into the plurality of reagent bottles; the fifth gas path is connected to the main gas path through a fourth three-way joint and is connected with the synthesis chamber in the synthesizer body so as to introduce inert gas into the synthesis chamber. The DNA synthesizer has the advantages of high automation degree and complete gas-liquid path.
Description
Technical Field
The invention relates to the field of DNA equipment, in particular to a DNA synthesizer.
Background
At present, the artificial synthesis of DNA is the only way for the known directional modification of gene sequences, and is widely applied to the fields of protein modification, life science and the like, such as nucleic acid medicine, enzyme engineering, gene detection, gene therapy and the like.
The DNA synthesizer needs deprotection, activation linking, capping, oxidation and other steps in the synthesis process, different reagents need to be injected into the synthesis column under the protection of inert gas in the synthesis process, and the reagents in the synthesis column are emptied after the reaction is completed. In the synthesis process, various reagents, operations such as cleaning liquid cleaning, inert gas cleaning and the like are often involved, the system is complex and inflexible to operate, and users cannot make proper adjustment according to the requirements.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art. Therefore, the invention provides the DNA synthesizer, which can provide a complete gas-liquid path system for the DNA synthesizer and improve the automation degree of the DNA synthesizer.
The present invention provides a DNA synthesizer, comprising:
the automatic reagent bottle cleaning device comprises a synthesizer body, wherein a blowing assembly, a plurality of reagent bottles and a cleaning electromagnetic valve group are arranged on the synthesizer body;
the gas storage tank is internally stored with inert gas;
the main gas circuit is connected between the synthesizer body and the gas storage tank;
the first gas path is connected to the main gas path through a first three-way joint and is connected with the synthesizer body so as to control the ejection of a piston on the blowing assembly;
the second air passage is connected to the main air passage through a second tee joint and is connected with an air outlet of the piston on the air blowing assembly;
the third gas path is connected to the main gas path through a third three-way joint and is connected with the cleaning solenoid valve group;
the fourth gas path is connected to the main gas path through a fourth three-way joint and is connected with the plurality of reagent bottles so as to lead the inert gas into the plurality of reagent bottles;
and the fifth gas path is connected to the main gas path through the fourth three-way joint and is connected with the synthesis chamber in the synthesizer body so as to introduce the inert gas into the synthesis chamber.
According to the DNA synthesizer provided by the embodiment of the invention, the first air path to the fifth air path are respectively arranged on the main air path, the first air path control piston is ejected in a targeted manner, the second air path control piston is blown, the third air path supplies air to the cleaning solenoid valve set, the fourth air path fills inert gas into the reagent bottle, the fifth air path feeds the inert gas into the synthesizer body, so that the five different air paths can independently act and can act in a mutual correlation manner, and the automation degree of the DNA synthesizer is improved.
According to an embodiment of the present invention, a first two-position three-way electromagnetic valve is connected to the first air path, two outlets of the first two-position three-way electromagnetic valve are respectively connected to the synthesizer body and an inlet of a fifth three-way joint, and two outlets of the fifth three-way joint are respectively connected to the piston to control the ejection of the piston.
According to one embodiment of the present invention, a first two-position two-way solenoid valve is further connected between one of the outlets of the fifth three-way joint and one of the pistons.
According to one embodiment of the invention, a sixth three-way joint is connected to the second gas path, and two outlets of the sixth three-way joint are respectively connected with the second two-position three-way electromagnetic valve and the third two-position three-way electromagnetic valve;
one outlet of the second two-position three-way electromagnetic valve is connected with the synthesizer body, and the other outlet of the second two-position three-way electromagnetic valve is connected with one outlet of the third two-position three-way electromagnetic valve through a seventh three-way joint;
the other outlet of the third two-position three-way electromagnetic valve is connected with the synthesizer body;
and an outlet of the seventh three-way joint is connected with an inlet of an eighth three-way joint, and two outlets of the eighth three-way joint are respectively connected with the piston so as to control the piston to blow air.
According to an embodiment of the present invention, a second two-position two-way solenoid valve is further connected between one of the outlets of the eighth three-way joint and one of the pistons.
According to one embodiment of the present invention, the reagent bottles are divided into two groups, and the first group of the reagent bottles and the second group of the reagent bottles are respectively connected to the fourth air path through a ninth three-way joint;
a first two-way ball valve and a first multi-way shunting block are sequentially connected between the ninth three-way joint and the first group of reagent bottles;
and a first three-way ball valve and a second multi-way shunting block are sequentially connected between the ninth three-way joint and the second group of reagent bottles.
According to one embodiment of the present invention, a first group of the reagent bottles contains at least four different reagents, and a second group of the reagent bottles contains at least five different reagents and a cleaning solution;
the first group of reagent bottles and the second group of reagent bottles are connected with an inlet of the cleaning electromagnetic valve group through pipelines, and an outlet of the cleaning electromagnetic valve group is connected with an inlet of a dispensing unit, so that the dispensing unit can inject reagents and/or cleaning liquid into the synthetic plate.
According to an embodiment of the present invention, a thirteenth through joint is further connected to a pipeline between the reagent bottle containing the cleaning solution and the cleaning solenoid valve set, and an outlet of the thirteenth through joint is connected to an inlet of the dispensing unit.
According to one embodiment of the invention, the synthesizer body is further provided with a movable tray;
the DNA synthesizer further comprises a waste discharge system, wherein the waste discharge system comprises:
the air pump is connected to the second multi-way flow splitting block through a second three-way ball valve;
and the waste liquid barrel is connected with the air pump through the other end of the second three-way ball valve and is communicated with the movable tray through a second two-way ball valve.
According to one embodiment of the invention, a third two-position two-way electromagnetic valve and a speed regulating valve are sequentially connected to the synthesizer body through the fourth three-way joint and the fifth gas path;
and a fourth two-position three-way electromagnetic valve is connected in parallel between the speed regulating valve and the synthesizer body.
One or more technical solutions in the embodiments of the present invention have at least one of the following technical effects:
according to the DNA synthesizer provided by the embodiment of the invention, the first air path to the fifth air path are respectively arranged on the main air path, the first air path control piston is ejected in a targeted manner, the second air path control piston is blown, the third air path controls the electromagnetic valve group to be cleaned, the fourth air path fills inert gas into the reagent bottle, the fifth air path feeds the inert gas into the synthesizer body, so that the five different air paths can independently act and can act in a mutual correlation manner, and the automation degree of the DNA synthesizer is improved.
Drawings
FIG. 1 is a schematic structural view of a DNA synthesizer according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a gas path and a liquid path of a DNA synthesizer according to an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a main gas circuit and first to fifth gas circuits of the DNA synthesizer according to the embodiment of the present invention;
fig. 4 is a schematic structural diagram of a first air path provided in the embodiment of the present invention;
fig. 5 is a schematic structural diagram of a second air path provided in the embodiment of the present invention;
fig. 6 is a schematic structural diagram of a fourth air path provided in the embodiment of the present invention.
The reference numbers illustrate:
100. a synthesizer body; 102. a blowing assembly; 104. a reagent bottle; 106. cleaning the electromagnetic valve group; 108. a gas storage tank; 110. a main gas path; 112. a first gas path; 114. a first three-way joint; 116. a second gas path; 118. a second three-way joint; 120. a third gas path; 122. a third three-way joint; 124. a fourth gas path; 126. a fourth three-way joint; 128. a fifth gas path; 130. a first two-position three-way solenoid valve; 132. a fifth three-way joint; 134. a first two-position two-way solenoid valve; 136. a sixth three-way joint; 138. a second two-position three-way electromagnetic valve; 140. a third two-position three-way electromagnetic valve; 142. a seventh three-way joint; 144. an eighth three-way joint; 146. a second two-position two-way solenoid valve; 148. a ninth three-way joint; 150. a first two-way ball valve; 152. a first multi-pass diverter block; 154. a first three-way ball valve; 156. a second multi-pass diverter block; 158. a dispensing unit; 160. a thirteenth joint; 162. an air pump; 164. a waste liquid barrel; 166. a third two-position two-way electromagnetic valve; 168. a speed regulating valve; 170. a fourth two-position three-way electromagnetic valve; 172. a second three-way ball valve; 174. a second two-way ball valve.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described below with reference to the accompanying drawings, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the embodiments of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the embodiments of the present invention and simplifying the description, but do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the embodiments of the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the embodiments of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "connected" and "connected" are to be interpreted broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. Specific meanings of the above terms in the embodiments of the present invention can be understood in specific cases by those of ordinary skill in the art.
In embodiments of the invention, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through intervening media. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of an embodiment of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
As shown in fig. 1 to 6, the present invention provides a DNA synthesizer, which includes a synthesizer body 100, an air storage tank 108, a main air channel 110, a first air channel 112, a second air channel 116, a third air channel 120, a fourth air channel 124 and a fifth air channel 128; wherein, the synthesizer body 100 is provided with a blowing assembly 102, a plurality of reagent bottles 104 and a cleaning solenoid valve set 106; the gas storage tank 108 stores inert gas; the main gas path 110 is connected between the synthesizer body 100 and the gas storage tank 108; the first air passage 112 is connected to the main air passage 110 through a first three-way joint 114 and is connected to the synthesizer body 100 to control ejection of the piston on the blowing assembly 102; the second air passage 116 is connected to the main air passage 110 through a second three-way joint 118 and is connected to an air outlet of a piston on the air blowing assembly 102; the third air path 120 is connected to the main air path 110 through a third three-way joint 122 and is connected to the cleaning solenoid valve set 106; the fourth gas path 124 is connected to the main gas path 110 through a fourth three-way joint 126 and is connected to the plurality of reagent bottles 104 to introduce the inert gas into the plurality of reagent bottles 104; a fifth gas line 128 is connected to the main gas line 110 through a fourth three-way joint 126 and is connected to the synthesis chamber in the synthesizer body 100 to introduce an inert gas into the synthesis chamber.
According to the DNA synthesizer provided by the embodiment of the invention, the first air path 112 to the fifth air path 128 are respectively arranged on the main air path 110, the piston of the first air path 112 is controlled to eject in a targeted manner, the piston of the second air path 116 is controlled to blow air, the third air path 120 supplies air to the cleaning electromagnetic valve set 106, the fourth air path 124 fills inert gas into the reagent bottle 104, the fifth air path 128 feeds the inert gas into the synthesizer body 100, so that the five different air paths can independently act and can act in a mutual correlation manner, and the automation degree of the DNA synthesizer is improved.
Specifically, the synthesizer body 100 includes a housing main body, a blowing assembly 102, and a moving tray. The front end of the shell main body is provided with a synthesis chamber, the movable tray is placed in the synthesis chamber, and the pressing and sealing device is fixed at the upper left of the synthesis chamber.
Two synthetic plates are placed on the movable tray, and M multiplied by N hollow synthetic columns (M is more than or equal to 2, and N is more than or equal to 2) are arranged on the synthetic plates. The movable tray can move to the position right below the air blowing assembly 102, the air blowing assembly 102 is provided with four air inlets and two ejection pistons, each of the two ejection pistons is provided with an air outlet, the two air inlets respectively control ejection of the left ejection piston and the right ejection piston, and the other two air inlets are respectively connected with the air outlets of the left ejection piston and the right ejection piston. When the air inlet for controlling the ejection of the ejection piston is used for air inlet, the ejection piston is ejected out to tightly press the synthetic plate on the movable tray, a sealed space is formed between the upper part of the synthetic plate and the ejection piston, and when the air inlet communicated with the air outlet of the ejection piston is used for air inlet, the synthetic plate is inflated.
A plurality of reagent bottles 104 and a cleaning solenoid valve set 106 are further arranged on the synthesizer body 100, wherein a plurality of different reagents are contained in the plurality of reagent bottles 104 to meet different synthesis requirements; the solenoid valve set 106 is cleaned to prevent the steel needle in the DNA synthesizer from being clogged.
The gas tank 108 is provided beside the DNA synthesizer and stores therein inert gas. The gas tank 108 is connected to the DNA synthesizer via a main gas line 110.
The main air passage 110 is connected to a first air passage 112, a second air passage 116, a third air passage 120, a fourth air passage 124, and a fifth air passage 128 in this order.
Specifically, the first air passage 112 is connected to the main air passage 110 through a first three-way joint 114, and is connected to the synthesizer body 100 to control the ejection of the piston on the synthesizer body 100.
According to an embodiment of the present invention, a first two-position three-way solenoid valve 130 is connected to the first air path 112, two outlets of the first two-position three-way solenoid valve 130 are respectively connected to the synthesizer body 100 and an inlet of a fifth three-way joint 132, and two outlets of the fifth three-way joint 132 are respectively connected to a piston to control the ejection of the piston; a first two-position two-way solenoid valve 134 is also connected between one of the outlets of the fifth three-way joint 132 and one of the pistons.
The first air path 112 enters a port 1 of the first two-position three-way solenoid valve 130 after passing through a pressure regulating valve, the pressure regulating valve is installed on a left side panel of the housing main body, the first two-position three-way solenoid valve 130 is fixed on a rear bottom plate of the housing main body, a port 2 of the first two-position three-way solenoid valve 130 is connected to a three-way joint, one interface of the three-way joint is connected to an air inlet of the air blowing assembly 102, and the other interface of the three-way joint is connected to.
The pressure gauge is fixed on a front upper left corner panel of the shell main body, a No. 3 port of the first two-position three-way electromagnetic valve 130 is connected into a fifth three-way joint 132, one end of the fifth three-way joint 132 is connected into one of left air inlets which are arranged on the air blowing assembly 102 and used for controlling ejection of a left ejection piston, and the other end of the fifth three-way joint 132 is connected into a P end of the first two-position two-way electromagnetic valve 134. The first two-position two-way solenoid valve 134 is a normally open solenoid valve, the port 2 and the port 3 of the first two-position three-way solenoid valve 130 are normally open, when power is on, the port 1 and the port 3 are communicated, the port 2 and the port 3 are disconnected, inert gas enters the blowing assembly 102, when the first two-position three-way solenoid valve 130 is powered off, the port 1 and the port 3 are disconnected, the port 2 and the port 3 are communicated, and gas in the compression sealing and inflation device enters the synthesis chamber; when the first two-position two-way solenoid valve 134 is not powered and the first two-position three-way solenoid valve 130 is powered, the left ejection piston and the right ejection piston of the air blowing assembly 102 are ejected simultaneously, and when the first two-position two-way solenoid valve 134 and the first two-position three-way solenoid valve 130 are powered simultaneously, only the left ejection piston on the air blowing assembly 102 is ejected, and the right ejection piston is not ejected, so that independent control is realized.
The second air passage 116 is connected to the main air passage 110 through a second three-way joint 118 and is connected to the synthesizer body 100 to control the piston blow air on the synthesizer body 100.
According to an embodiment of the present invention, a sixth three-way joint 136 is connected to the second air path 116, and two outlets of the sixth three-way joint 136 are respectively connected to a second two-position three-way solenoid valve 138 and a third two-position three-way solenoid valve 140; one outlet of the second two-position three-way solenoid valve 138 is connected with the synthesizer body 100, and the other outlet of the second two-position three-way solenoid valve 138 is connected with one outlet of the third two-position three-way solenoid valve 140 through a seventh three-way joint 142; the other outlet of the third two-position three-way solenoid valve 140 is connected with the synthesizer body 100; an outlet of the seventh three-way joint 142 is connected with an inlet of the eighth three-way joint 144, and two outlets of the eighth three-way joint 144 are respectively connected with the piston to control the piston to blow air; a second two-position two-way solenoid valve 146 is also connected between one of the outlets of the eighth three-way joint 144 and one of the pistons.
The second air path is connected to a sixth three-way joint 136 after passing through a pressure regulating valve, the pressure regulating valve is installed on the left side panel of the shell main body, two interfaces of the sixth three-way joint 136 respectively enter inlets of a second two-position three-way electromagnetic valve 138 and a third two-position three-way electromagnetic valve 140, and the second two-position three-way electromagnetic valve 138 and the third two-position three-way electromagnetic valve 140 are fixed on the rear bottom plate of the shell main body.
One outlet of the second two-position three-way solenoid valve 138 is connected to one end of the one-way speed regulating valve 168, the third two-position three-way solenoid valve 140 is connected to the other end of the one-way speed regulating valve 168 through a seventh three-way joint 142, meanwhile, the remaining interface of the seventh three-way joint 142 is connected to another three-way joint, one end of the three-way joint is connected to the eighth three-way joint 144, and the other end is connected to the pressure gauge.
One end of the eighth three-way joint 144 is directly connected to the air outlet of the air blowing assembly 102 for controlling the air blowing of the left ejection piston, the other end of the eighth three-way joint is connected to the P end of the second two-position two-way solenoid valve 146, the second two-position two-way solenoid valve 146 is a normally open solenoid valve, and the Q end of the second two-position two-way solenoid valve 146 is connected to the air outlet of the air blowing assembly 102 for controlling the air blowing of the right ejection piston. When the second two-position three-way solenoid valve 138 is powered on, the inert gas directly enters the air outlets of the ejection pistons on the left side and the right side of the air blowing assembly 102 without speed regulation, and simultaneously blows the atmosphere (when the second two-position two-way solenoid valve 146 is powered on, only the ejection piston on the left side blows the atmosphere); when the power is cut off, the air blowing is stopped; when the third two-position three-way solenoid valve 140 is powered on, the air enters the air outlets of the ejection pistons on the left side and the right side of the air blowing assembly 102 to blow small air after the air quantity is adjusted by the one-way speed adjusting valve 168 (when the second two-position two-way solenoid valve 146 is powered on, only the ejection piston on the left side blows small air), and when the power is off, the small air blowing is stopped. When the second two-position three-way solenoid valve 138 is energized and the third two-position three-way solenoid valve 140 is not energized, the inert gas enters the outlet of the third two-position three-way solenoid valve 140 connected with the synthesizer body 100, and then directly enters the synthesis cavity.
The fourth gas path 124 is connected to the main gas path 110 through a fourth three-way joint 126 and is connected to the plurality of reagent bottles 104 to introduce the inert gas into the plurality of reagent bottles 104.
According to one embodiment of the present invention, the reagent bottles 104 are divided into two groups, and the first group of reagent bottles 104 and the second group of reagent bottles 104 are respectively connected to the fourth air path 124 through the ninth three-way joint 148; a first two-way ball valve 150 and a first multi-way flow splitting block 152 are sequentially connected between the ninth three-way joint 148 and the first group of reagent bottles 104; a first three-way ball valve 154 and a second multi-way flow splitting block 156 are sequentially connected between the ninth three-way joint 148 and the second group of reagent bottles 104; the first set of reagent bottles 104 contains at least four different reagents, and the second set of reagent bottles 104 contains at least five different reagents and cleaning solutions; the first group of reagent bottles 104 and the second group of reagent bottles 104 are connected with the inlet of the cleaning solenoid valve set 106 through a pipeline, and the outlet of the cleaning solenoid valve set 106 is connected with the inlet of the dispensing unit 158, so that the dispensing unit 158 injects the reagent and/or the cleaning solution into the synthetic plate; a thirteenth connection joint 160 is connected to a pipe between the reagent bottle 104 containing the cleaning liquid and the cleaning solenoid valve block 106, and an outlet of the thirteenth connection joint 160 is connected to an inlet of the dispensing unit 158.
Specifically, a check valve is connected to the fourth air path after passing through the pressure regulating valve, the check valve prevents the inert gas from flowing back, the pressure regulating valve is installed on a left panel of the shell main body, a three-way joint is connected to the back of the check valve, one interface of the three-way joint is connected to a pressure gauge, the pressure gauge is installed on a front upper left corner panel of the shell main body, and the other interface of the three-way joint is connected to a ninth three-way joint 148. One end of a ninth three-way joint 148 is connected with a first two-way ball valve 150, the first two-way ball valve 150 is installed on the left rear side panel of the shell main body, the other end of the first two-way ball valve 150 is connected with the P3 end of the first three-way ball valve 154, the first two-way ball valve 150 is connected into one interface of the first multi-way flow splitting block 152, and the P2 end of the first three-way ball valve 154 is connected into one interface of the second multi-way flow splitting block 156; when the knob of the first three-way ball valve 154 points to the end P3, the end P3 is communicated with the end P2, and the inert gas enters the first multi-way diverter block 152; when the first three-way ball valve 154 is turned with the handle pointing to the end P1, the inert gas is interrupted from entering the second manifold block 156 and the gas in the second manifold block 156 is vented to the atmosphere.
The remaining ports of the first multi-way flow splitting block 152 are respectively connected with the air inlets of the first group of reagent bottles 104 which are connected in pairs, wherein the first group of reagent bottles 104 comprises 8 reagent bottles 104 which are connected in pairs, 4 reagent bottles 104 are arranged on the left side of the housing main body, and the other 4 reagent bottles 104 are arranged on the right side of the housing main body. Two liang of 4 reagent bottles 104 on left side and 4 reagent bottles 104 on right side link to each other, in two reagent bottles 104 that two liang link to each other, every reagent bottle 104 all has an air inlet, a liquid outlet, the air inlet of installing on the left reagent bottle 104 of shell main part links to each other with the interface on the first many-way flow distribution piece 152, the liquid outlet links to each other with the air inlet of installing on the reagent bottle 104 on shell main part right side, the liquid outlet of installing on the reagent bottle 104 on shell main part right side links to each other with the inlet of wasing solenoid valve group 106, so, total 4 interfaces on the first many-way flow distribution piece 152 link to each other with the air inlet of first group reagent bottle 104 respectively, remaining interface passes through the. Of course, the number of the reagent bottles 104 may be increased according to actual needs, the liquid outlets of the reagent bottles 104 installed on the right side of the housing main body in the 4 groups of reagent bottles 104 are respectively connected to 4 liquid inlets of the cleaning solenoid valve set 106, and the 4 groups of reagent bottles 104 respectively store A, B, C, D four reagents.
The interface of the second multi-way flow splitting block 156 is connected with another 6 groups of reagent bottles 104 which are connected pairwise, each reagent bottle 104 in the group of reagent bottles 104 also has an air inlet and a liquid outlet, the air inlet of one reagent bottle 104 is connected with the interface of the second multi-way flow splitting block 156, the liquid outlet is connected with the air inlet of another reagent bottle 104, the liquid outlet of another reagent bottle 104 is connected with the liquid inlet of the cleaning electromagnetic valve group 106, one of the interfaces of the second multi-way flow splitting block 156 is connected with the discharge system, and the rest interfaces are blocked by plugs. The 6 groups of reagent bottles 104 connected two by two respectively store E, F, G, H, I and six kinds of cleaning solution. The liquid outlet of the reagent bottle 104 containing the reagent E to I is correspondingly connected to the liquid inlet of the cleaning electromagnetic valve set 106, the liquid outlet of the reagent bottle 104 containing the cleaning liquid is divided into two paths by a thirteenth joint 160, one path is connected to the cleaning liquid inlet of the cleaning electromagnetic valve set 106, and the other path is directly connected to the liquid inlet of the cleaning liquid electromagnetic valve set of the dispensing unit 158. Thus, the reagent storage capacity can be increased, the number of times of replacing the reagent bottle 104 can be reduced, and the reagent replacement by interrupting the synthesis procedure in the synthesis process can be avoided.
In the embodiment of the invention, the cleaning electromagnetic valve mainly comprises an electromagnetic valve seat, ten two-position three-way electromagnetic valves and an inner hexagonal plug. Wherein, the electromagnetic valve seat is provided with a main channel, the liquid inlet at the end part is blocked by a hexagonal plug, and two side surfaces of the electromagnetic valve seat are respectively provided with 10 liquid inlets of A-I, cleaning liquid and the like, 9 liquid outlets of A-I and the like and an inert gas inlet. The 9 reagents A to I are respectively fed into the liquid inlets A to I and flow out of the liquid outlets A to I, and respectively and correspondingly enter the 9 groups of liquid pumping electromagnetic valve groups A to I of the separate injection unit 158.
The dispensing unit 158 includes 10 sets of a dispensing solenoid valve set, a dispensing needle plate and a dispensing capillary tube, i.e., a to I and a cleaning liquid set. Wherein, every group beats liquid solenoid valve group and contains a base, and N two lead to solenoid valve (N is greater than or equal to 2), have a hickey on the base minor face terminal surface, and this hickey directly links to each other with a main entrance, and there is N hickey (N is greater than or equal to 2) a base side, and every two lead to solenoid valve control main entrance communicates with each other with a hickey of side, and when corresponding two lead to solenoid valve was electrified, the main entrance communicates with the screw hole of side, and when cutting off the power supply, the main entrance does not communicate with the screw hole of side.
The liquid injection needle plate is provided with 10 rows of threaded holes, each row is provided with N threaded holes (N is more than or equal to 2), and the bottom of each threaded hole is inserted with a liquid injection needle head. And the threaded interface on the side surface of each group of the liquid injection solenoid valve set fixes one end of a liquid outlet capillary tube through a clamping sleeve joint, the other end of the liquid outlet capillary tube is fixed in the threaded hole of each row of the liquid injection needle plate through a clamping sleeve joint, and the N threaded interfaces on the side surface of the liquid injection solenoid valve set are communicated with the N liquid injection needles on each row of the liquid injection needle plate.
According to an embodiment of the present invention, the synthesizer body 100 is further provided with a movable tray; the DNA synthesizer further comprises a waste discharge system, wherein the waste discharge system comprises an air pump 162 and a waste liquid barrel 164; wherein, the air pump 162 is connected to the second multi-way diverter block 156 through a second three-way ball valve 172; the waste liquid barrel 164 is connected to the air pump 162 through the other end of the second three-way ball valve 172, and the waste liquid barrel 164 is communicated with the movable tray through the second two-way ball valve 174.
Specifically, the waste discharge system includes an air pump 162 and a waste liquid tank 164, the air pump 162 mainly provides suction and discharges waste gas, and the waste liquid tank 164 has a liquid inlet, a suction opening and a liquid outlet. The air pump 162 is connected to the second manifold block 156 via a second three-way ball valve 172, and the waste tank 164 is connected to the movable tray via a second two-way ball valve 174. During normal operation, P2 and the end switch-on of P3 of second three-way ball valve 172, second two-way ball valve 174 opens, and another two-way ball valve of connection on waste liquid bucket 164 closes, and air pump 162 circular telegram extracts the air in waste liquid bucket 164, and waste liquid bucket 164 produces certain negative pressure to waste gas, the waste liquid that will remove in tray and the synthetic cavity are inhaled in waste liquid bucket 164, and the waste liquid is stayed in waste liquid bucket 164, and waste gas passes through air pump 162 and discharges. When the waste liquid barrel 164 is fully loaded, the second two-way ball valve 174 is closed, the second three-way ball valves 172 to P1 are switched to be communicated with the P2, the air pump 162 is opened, the other two-way ball valve on the waste liquid barrel 164 is opened, the air inlet end of the whole system inflates air to the waste liquid barrel 164, and positive pressure is generated to discharge waste liquid.
A fifth gas line 128 is connected to the main gas line 110 through a fourth three-way joint 126 and is connected to the synthesis chamber in the synthesizer body 100 to introduce an inert gas into the synthesis chamber.
According to an embodiment of the present invention, a third two-position two-way solenoid valve 166 and a speed regulating valve 168 are sequentially connected to the synthesizer body 100 through a fourth three-way joint 126 and the fifth air path 128; a fourth two-position three-way solenoid valve 170 is also connected in parallel between the speed valve 168 and the synthesizer body 100.
The fifth gas path is directly connected to the gas inlet end of the third two-position two-way solenoid valve 166, the gas outlet end is connected to the a end of the one-way speed control valve 168, the b end of the one-way speed control valve 168 is connected to the port 3 of the fourth two-position three-way solenoid valve 170, the port 2 of the fourth two-position three-way solenoid valve 170 is connected to the a end of the one-way speed control valve 168, the b end of the one-way speed control valve 168 is connected to a three-way joint, the other interface of the three-way joint is connected to the port 1 of the fourth two-position three-way solenoid valve 170, and the remaining interface. When the third two-position two-way solenoid valve 166 is electrified and the fourth two-position three-way solenoid valve 170 is not electrified, a small amount of protective gas is filled in the synthesis chamber; when the third two-position two-way solenoid valve 166 and the fourth two-position three-way solenoid valve 170 are simultaneously electrified, a large amount of protective gas is filled in the synthesis chamber; when the third two-position two-way solenoid 166 is de-energized, the charging stops.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A DNA synthesizer, comprising:
the device comprises a synthesizer body (100), wherein a blowing assembly (102), a plurality of reagent bottles (104) and a cleaning solenoid valve set (106) are arranged on the synthesizer body (100);
a gas tank (108), wherein inert gas is stored in the gas tank (108);
a main gas path (110) connected between the synthesizer body (100) and the gas tank (108);
the first air path (112) is connected to the main air path (110) through a first three-way joint (114) and is connected with the synthesizer body (100) so as to control ejection of a piston on the blowing assembly (102);
the second air path (116) is connected to the main air path (110) through a second three-way joint (118) and is connected with an air outlet of the piston on the air blowing assembly (102);
the third air path (120) is connected to the main air path (110) through a third three-way joint (122) and is connected with the cleaning solenoid valve group (106);
a fourth gas path (124) connected to the main gas path (110) through a fourth three-way joint (126) and connected to the plurality of reagent bottles (104) to introduce the inert gas into the plurality of reagent bottles (104);
and the fifth gas path (128) is connected to the main gas path (110) through the fourth three-way joint (126) and is connected with a synthesis chamber in the synthesizer body (100) so as to lead the inert gas into the synthesis chamber.
2. The DNA synthesizer of claim 1, wherein a first two-position three-way solenoid valve (130) is connected to the first air channel (112), two outlets of the first two-position three-way solenoid valve (130) are respectively connected to the synthesizer body (100) and an inlet of a fifth three-way joint (132), and two outlets of the fifth three-way joint (132) are respectively connected to the piston to control the ejection of the piston.
3. The DNA synthesizer according to claim 2, characterized in that a first two-position two-way solenoid valve (134) is further connected between one of the outlets of the fifth three-way connection (132) and one of the pistons.
4. The DNA synthesizer according to claim 1, wherein a sixth three-way joint (136) is connected to the second gas path (116), and two outlets of the sixth three-way joint (136) are respectively connected to a second two-position three-way solenoid valve (138) and a third two-position three-way solenoid valve (140);
one outlet of the second two-position three-way solenoid valve (138) is connected with the synthesizer body (100), and the other outlet of the second two-position three-way solenoid valve (138) is connected with one outlet of the third two-position three-way solenoid valve (140) through a seventh three-way joint (142);
the other outlet of the third two-position three-way electromagnetic valve (140) is connected with the synthesizer body (100);
an outlet of the seventh three-way joint (142) is connected with an inlet of an eighth three-way joint (144), and two outlets of the eighth three-way joint (144) are respectively connected with the piston so as to control the piston to blow air.
5. The DNA synthesizer according to claim 4, characterized in that a second two-position two-way solenoid valve (146) is further connected between one of the outlets of the eighth three-way joint (144) and one of the pistons.
6. The DNA synthesizer according to claim 1, wherein the reagent bottles (104) are divided into two groups, and the first group of the reagent bottles (104) and the second group of the reagent bottles (104) are connected to the fourth air path (124) through a ninth three-way joint (148), respectively;
a first two-way ball valve (150) and a first multi-way flow splitting block (152) are sequentially connected between the ninth three-way joint (148) and the first group of reagent bottles (104);
and a first three-way ball valve (154) and a second multi-way flow splitting block (156) are sequentially connected between the ninth three-way joint (148) and the second group of reagent bottles (104).
7. The DNA synthesizer according to claim 6, wherein a first group of the reagent bottles (104) contains at least four different reagents, and a second group of the reagent bottles (104) contains at least five different reagents and a cleaning solution;
the first group of reagent bottles (104) and the second group of reagent bottles (104) are connected with the inlet of the cleaning electromagnetic valve group (106) through pipelines, the outlet of the cleaning electromagnetic valve group (106) is connected with the inlet of a dispensing unit (158), so that the dispensing unit (158) injects the reagent and/or the cleaning solution into the synthetic plate.
8. The DNA synthesizer according to claim 7, wherein a thirteenth connection joint (160) is further connected to a pipeline between the reagent bottle (104) containing the cleaning solution and the cleaning solenoid valve block (106), and an outlet of the thirteenth connection joint (160) is connected to an inlet of the dispensing unit (158).
9. The DNA synthesizer according to claim 7, wherein a moving tray is further provided on the synthesizer body (100);
the DNA synthesizer also comprises a waste discharge system,
the waste discharge system comprises:
an air pump (162) connected to the second manifold block (156) by a second three-way ball valve (172);
and the waste liquid barrel (164) is connected with the air pump (162) through the other end of the second three-way ball valve (172), and the waste liquid barrel (164) is communicated with the movable tray through a second two-way ball valve (174).
10. The DNA synthesizer according to claim 1, characterized in that a third two-position two-way solenoid valve (166) and a speed regulating valve (168) are connected to the fifth air channel (128) from the fourth three-way joint (126) to the synthesizer body (100) in sequence;
and a fourth two-position three-way electromagnetic valve (170) is connected in parallel between the speed regulating valve (168) and the synthesizer body (100).
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