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CN111929431B - Fluorescence immunoassay quantitative analyzer - Google Patents

Fluorescence immunoassay quantitative analyzer Download PDF

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Publication number
CN111929431B
CN111929431B CN202011018330.5A CN202011018330A CN111929431B CN 111929431 B CN111929431 B CN 111929431B CN 202011018330 A CN202011018330 A CN 202011018330A CN 111929431 B CN111929431 B CN 111929431B
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groove
reagent
module
piece
lead screw
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CN111929431A (en
Inventor
肖扬赟
蔡志文
刘银艳
刘子龙
董燕
周华茂
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Nanchang Maote Biotechnology Co ltd
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Nanchang Maote Biotechnology Co ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302Apparatus specially adapted for immunological test procedures
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531Production of immunochemical test materials
    • G01N33/532Production of labelled immunochemicals
    • G01N33/533Production of labelled immunochemicals with fluorescent label

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The invention discloses a fluorescence immunoassay quantitative analyzer, and relates to the technical field of devices for detecting biological activity in laboratories. The problem of pollution to the inside of the fluorescence immunoassay quantitative analyzer is solved because the sample is easy to incline or drop when the sample diluent is excessively added. The utility model discloses a reagent piece detection device, including casing, ID card reading groove, cleaning roller, reagent piece detection groove, display screen, reagent piece, cleaning roller and ultraviolet lamp, the intermediate position department of casing top is provided with the display screen, one side of display screen is provided with the ID card reading groove, one side of casing is provided with the reagent piece groove, the inboard in reagent piece groove is provided with the reagent piece and detects the groove, the inside in reagent piece detection groove is provided with the cleaning roller, and the inner wall in cleaning roller and reagent piece detection groove laminates mutually, one side.

Description

Fluorescence immunoassay quantitative analyzer
Technical Field
The invention relates to a biochemical analysis and determination technology, in particular to a fluorescence immunoassay quantitative analyzer.
Background
A fluoroimmunoassay quantitative analyzer is an instrument for measuring bioactive compounds containing very low amounts of BAI. An immunofluorescence quantitative analyzer is used for carrying out rapid and accurate quantitative analysis on the content of various analytes (CRP, PCT, NT-proBNP, cTnI and the like) in human blood and urine. The light source adopts a high-power LED lamp bead, a narrow-band interference filter is adopted to filter excitation light and fluorescence, and a photoelectric conversion chip OPT101 with a built-in operational amplifier is adopted to detect fluorescence intensity. The wide-body slide block with double rows of balls is driven by a stepping motor and slides on a single precise linear guide rail in a belt transmission mode, so that scanning detection of a detection sample is realized. The result of a comparison test with a standard instrument shows that the prototype has accurate measurement result, high measurement precision and good stability on the basis of miniaturization, rapidness and low cost, and can meet the requirements of clinical application. There is therefore a great need in the market for a network-based meter display to address these problems.
Examples of the conventional fluorescence immunoassay analyzer include CN207717626U and CN 111579766A. Need to drip the reagent piece that has the sample diluent when this kind of equipment detects and place into the instrument and carry out the assay, nevertheless just easily because of the gripping slope or cause the drippage when sample diluent adds excessively to cause the pollution to the inside of fluorescence immunoassay quantitative analysis appearance, consequently urgently need fluorescence immunoassay quantitative analysis appearance to solve these problems in the market.
Disclosure of Invention
The present invention aims to provide a quantitative fluorescence immunoassay analyzer, which solves the problem of contamination to the interior of the quantitative fluorescence immunoassay analyzer caused by inclination of grasping or dropping when too much diluent is added to a sample.
In order to achieve the purpose, the invention provides the following technical scheme: the fluorescence immunoassay quantitative analyzer comprises a shell, wherein a display screen is arranged at the middle position above the shell, an ID card reading groove is arranged at one side of the display screen, a reagent sheet groove is arranged at one side of the shell, a reagent sheet detection groove is arranged at the inner side of the reagent sheet groove, a cleaning roller is arranged inside the reagent sheet detection groove and is attached to the inner wall of the reagent sheet detection groove, an ultraviolet lamp is fixedly arranged at one side of the reagent sheet detection groove, a control main board is arranged at the middle position inside the shell, a photoelectric module is arranged above the reagent sheet detection groove, a smart card reading and writing module is arranged at the upper end of the photoelectric module, an audio module is arranged at one side of the smart card reading and writing module, the output end of the control main board is electrically connected with the input end of the audio module, and a lead screw is arranged at one side of the reagent sheet detection groove, one side of lead screw is provided with lead screw motor, the below of lead screw is provided with electric telescopic handle, and electric telescopic handle and the equal fixed connection of lead screw and casing, the outside cover of lead screw is equipped with the slider, and the slider has set gradually threely, and slider and lead screw sliding connection, pass through connecting rod fixed connection between slider and the cleaning roller.
Preferably, the reagent piece groove is provided with three, and three reagent piece groove equidistance distributes, reagent piece groove and ID read card groove correspond the setting.
Preferably, one side of control mainboard is provided with printing module, one side of printing module is provided with beats printer paper and rolls up the groove, beat printer paper and roll up one side in groove and be provided with the printing apron, and print the apron and rotate with the casing and be connected, one side of control mainboard and printing module is provided with power module, and print module and power module and the equal fixed connection of casing, one side in reagent piece groove is provided with the status light, and status light and control mainboard electric connection.
Preferably, the smart card reading and writing module is located below the ID reading and writing slot, the smart card reading and writing module and the audio module are fixedly connected with the shell, and the photoelectric module is electrically connected with the control mainboard in a bidirectional mode.
Preferably, the control mainboard is fixedly connected with the shell, and the electric telescopic rod and the screw motor are electrically connected with the control mainboard.
Preferably, one side that the reagent piece detects the groove and is close to ultraviolet lamp is provided with isolation glass, and isolation glass and reagent piece detect groove sealing connection, one side that the reagent piece detects the groove and is close to the reagent piece groove is provided with the baffle, the upper end of baffle is connected through pivot and casing rotation.
Preferably, a spring piece is arranged between the upper end of the baffle and the inner wall of the reagent plate detection groove, one end of the spring piece is fixedly connected with the upper end of the baffle, and the other end of the spring piece is fixedly connected with the inner wall of the reagent plate detection groove.
Preferably, the printing module and the ID card reading slot are electrically connected with a control mainboard, and the output end of the control mainboard is electrically connected with the input end of the display screen.
Compared with the prior art, the invention has the beneficial effects that:
1. the cleaning roller is arranged in the reagent plate detection groove for effective cleaning, the cleaning roller is made of sponge materials, so that splashed sample diluent can be well absorbed, the influence on detection is avoided, the cleaning in the reagent plate detection groove can be effectively kept, dust is prevented from entering and influencing the collection work of a photoelectric module on a reagent plate, when the reagent plate detection groove is not cleaned, the insertion of the reagent plate is not influenced, meanwhile, the reagent plate groove is blocked by the baffle plate, the dust can be effectively prevented from entering when the reagent plate is not detected, the cleaning work of the cleaning roller is controlled by the matching of the lead screw and the electric telescopic rod, so that the inner wall of the reagent plate detection groove can be effectively cleaned without dead corners, and the problem that when the sample diluent drops are too much, the cleaning roller is easy to incline due to grasping or drop is solved, thereby causing a problem of contamination of the inside of the quantitative fluorescence immunoassay analyzer.
2. According to the invention, the photoelectric module is arranged to realize optical detection on the reagent sheet, the reagent sheet is sent to the control main board for result analysis after detection, the printing module is used for printing, and after printing is finished, a worker often forgets to take the ID card, so that the ID card is easy to lose.
Drawings
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a schematic structural view of the present invention;
FIG. 3 is a view showing the connection between a reagent sheet measuring well and a feed screw according to the present invention;
FIG. 4 is a view showing the connection between the reagent sheet measuring well and the housing according to the present invention;
fig. 5 is a partially enlarged view of the region a of the present invention.
In the figure: 1. a housing; 2. a display screen; 3. an ID card reading slot; 4. a reagent strip slot; 5. a control main board; 6. a printing module; 7. a power supply module; 8. a smart card read-write module; 9. an audio module; 10. printing a cover plate; 11. a photovoltaic module; 12. a lead screw; 13. an electric telescopic rod; 14. a printing paper roll slot; 15. a lead screw motor; 16. an ultraviolet lamp; 17. a slider; 18. a connecting rod; 19. cleaning the roller; 20. a reagent sheet detection tank; 21. an insulating glass; 22. a baffle plate; 23. a spring plate; 24. a status light.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Referring to fig. 1-5, an embodiment of the present invention is shown: fluorescence immunity quantitative analysis appearance, which comprises a housin 1, the intermediate position department of casing 1 top is provided with display screen 2, one side of display screen 2 is provided with ID and reads draw-in groove 3, one side of casing 1 is provided with reagent piece groove 4, the inboard of reagent piece groove 4 is provided with reagent piece detection groove 20, reagent piece detection groove 20 is used for carrying out the monitoring of reagent piece, the inside of reagent piece detection groove 20 is provided with cleaning roller 19, cleaning roller 19 is used for wiping the inner wall of reagent piece detection groove 20, and cleaning roller 19 laminates with the inner wall of reagent piece detection groove 20 mutually, one side fixed mounting of reagent piece detection groove 20 has ultraviolet lamp 16, ultraviolet lamp 16 sets up can realize the disinfection to reagent piece detection groove 20 inboard.
Further, reagent piece groove 4 is provided with threely, and 4 equidistance distributions in three reagent piece grooves, and reagent piece groove 4 corresponds the setting with ID card reading groove 3, can carry out the detection of three groups of reagent pieces simultaneously through corresponding the setting.
Further, a control mainboard 5 is arranged at the middle position inside the casing 1, a printing module 6 is arranged on one side of the control mainboard 5, the model of the printing module 6 is HDP-II, a printing paper roll groove 14 is arranged on one side of the printing module 6, a printing cover plate 10 is arranged on one side of the printing paper roll groove 14, the printing cover plate 10 is rotatably connected with the casing 1, a power supply module 7 is arranged on one side of the control mainboard 5 and one side of the printing module 6, the power supply module 7 and the casing 1 are fixedly connected, a status lamp 24 is arranged on one side of the reagent sheet groove 4, the status lamp 24 is electrically connected with the control mainboard 5, the status lamp 24 can be directly controlled through electrical setting, and whether detection work can be carried out at the moment is visually displayed.
Further, a photoelectric module 11 is arranged above the reagent sheet detection groove 20, the photoelectric module 11 is used for detection, a smart card reading and writing module 8 is arranged at the upper end of the photoelectric module 11, the type of the smart card reading and writing module 8 is B50FA-T, the smart card reading and writing module 8 is used for reading an ID card, the smart card reading and writing module 8 is located below the ID card reading and writing groove 3, an audio module 9 is arranged on one side of the smart card reading and writing module 8, the audio module 9 is used for playing an early warning sound, the smart card reading and writing module 8 and the audio module 9 are fixedly connected with the shell 1, the output end of the control mainboard 5 is electrically connected with the input end of the audio module 9, the photoelectric module 11 is electrically connected with the control mainboard 5 in a bidirectional mode, and the electrical connection realizes transmission of electrical signals.
Further, one side of reagent piece detection groove 20 is provided with lead screw 12, lead screw 12 is used for adjusting the position of cleaning roller 19, one side of lead screw 12 is provided with screw motor 15, the below of lead screw 12 is provided with electric telescopic handle 13, and electric telescopic handle 13 and lead screw 12 and the equal fixed connection of casing 1, the outside cover of lead screw 12 is equipped with slider 17, slider 17 has set gradually threely, and slider 17 and lead screw 12 sliding connection, through connecting rod 18 fixed connection between slider 17 and the cleaning roller 19, control mainboard 5 and casing 1 fixed connection, and electric telescopic handle 13 and screw motor 15 and control mainboard 5 electric connection, realized the control to electric telescopic handle 13 and screw motor 15 through electric connection.
Further, one side that reagent piece detects groove 20 and is close to ultraviolet lamp 16 is provided with isolation glass 21, and isolation glass 21 and reagent piece detect groove 20 sealing connection, and one side that reagent piece detects groove 20 and is close to reagent piece groove 4 is provided with baffle 22, and the upper end of baffle 22 is rotated through pivot and casing 1 and is connected, can effectually prevent the entering of dust through the setting of baffle 22.
Further, a spring piece 23 is provided between the upper end of the baffle plate 22 and the inner wall of the reagent piece detecting well 20. One end of the spring piece 23 is fixedly connected with the upper end of the baffle 22, the other end of the spring piece is fixedly connected with the inner wall of the reagent plate detection groove 20, and the baffle 22 can be effectively limited by the arrangement of the spring piece 23.
Further, the printing module 6 and the ID card reading slot 3 are electrically connected with the control mainboard 5, the output end of the control mainboard 5 is electrically connected with the input end of the display screen 2, and data transmission is realized through the electrical connection.
The working principle is as follows: when the intelligent card reader-writer is used, a worker inserts a reagent sheet dripped with a sample diluent into the reagent sheet groove 4, due to the insertion of the reagent sheet, when the pushing force is larger than the elastic force of the spring piece 23, the baffle plate 22 is enabled to turn towards the inside of the reagent sheet detection groove 20, after the reagent sheet enters, the intelligent card read-write module 8 below the ID card reading groove 3 reads the ID card, the control mainboard 5 limits the read content through the display screen 2, after the user determines that the read content is limited, the photoelectric module 11 reads the reagent sheet and sends the read image to the control mainboard 5, the control mainboard 5 analyzes the image, the analyzed value is displayed through the display screen 2, and simultaneously the printing module 6 prints, at the moment, the intelligent card read-write module 8 monitors the ID card in time, and controls the mainboard 5 to time, when the ID card is not taken after ten seconds, the audio module 9 sounds, therefore, loss of the ID card is prompted by a person mode, after detection is finished, the detection sheet is taken out, the control main board 5 can control the state lamp 24 to light up to prompt a worker that the reagent sheet detection groove 20 is being cleaned, at the moment, the electric telescopic rod 13 extends firstly to enable the cleaning roller 19 to move upwards along the inner wall of one side of the reagent sheet detection groove 20, when the reagent sheet detection groove is moved to a set position, the position of the electric telescopic rod 13 is locked, at the moment, the lead screw motor 15 can be driven to rotate, the sliding block 17 on the lead screw 12 is driven to slide forwards through rotation of the lead screw motor 15, so that the cleaning roller 19 is driven to move forwards to clean the top of the reagent sheet detection groove 20, when the reagent sheet detection groove is moved to the set position, the lead screw motor 15 is stopped, at the moment, contraction of the electric telescopic rod 13 is carried out, at the moment, the cleaning roller 19 can clean, electric telescopic handle 13 stops, lead screw motor 15 overturns this moment, thereby drive cleaning roller 19 backward movement, clean the bottom that detects the groove 20 inner wall of reagent piece, thereby realized detecting holistic the cleaning in groove 20 to the reagent piece, stop lead screw motor 15 after arriving the setting position, carrying out electric telescopic handle 13's shrink, accomodate one side of reagent piece detection groove 20 below back with cleaning roller 19, this moment alright disinfect to the groove 20 inner wall of reagent piece with opening ultraviolet lamp 16.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (8)

1. Fluorescence immunity quantitative analysis appearance, including casing (1), its characterized in that: the device is characterized in that a display screen (2) is arranged at the middle position above the shell (1), an ID reading slot (3) is arranged at one side of the display screen (2), a reagent sheet groove (4) is arranged at one side of the shell (1), a reagent sheet detection groove (20) is arranged at the inner side of the reagent sheet groove (4), a cleaning roller (19) is arranged in the reagent sheet detection groove (20), the cleaning roller (19) is attached to the inner wall of the reagent sheet detection groove (20), an ultraviolet lamp (16) is fixedly arranged at one side of the reagent sheet detection groove (20), a control main board (5) is arranged at the middle position inside the shell (1), a photoelectric module (11) is arranged above the reagent sheet detection groove (20), an intelligent card reading and writing module (8) is arranged at the upper end of the photoelectric module (11), an audio module (9) is arranged at one side of the intelligent card reading and writing module (8), the output of control mainboard (5) and the input electric connection of audio module (9), one side that the reagent piece detected groove (20) is provided with lead screw (12), one side of lead screw (12) is provided with lead screw motor (15), the below of lead screw (12) is provided with electric telescopic handle (13), and electric telescopic handle (13) and lead screw (12) and the equal fixed connection of casing (1), the outside cover of lead screw (12) is equipped with slider (17), and slider (17) has set gradually threely, and slider (17) and lead screw (12) sliding connection, through connecting rod (18) fixed connection between slider (17) and cleaning roller (19).
2. The fluoroimmunoassay quantitative analyzer of claim 1, wherein: reagent piece groove (4) are provided with threely, and three reagent piece groove (4) equidistance distributes, reagent piece groove (4) read draw-in groove (3) with the ID and correspond the setting.
3. The fluoroimmunoassay quantitative analyzer of claim 1, wherein: one side of control mainboard (5) is provided with printing module (6), one side of printing module (6) is provided with beats printing paper and rolls up groove (14), beat printing paper and roll up one side of groove (14) and be provided with and print apron (10), and print apron (10) and casing (1) rotation connection, one side of control mainboard (5) and printing module (6) is provided with power module (7), and just prints module (6) and power module (7) and the equal fixed connection of casing (1), one side of reagent piece groove (4) is provided with status light (24), and status light (24) and control mainboard (5) electric connection.
4. The fluoroimmunoassay quantitative analyzer of claim 3, wherein: the intelligent card reading and writing module (8) is located below the ID card reading groove (3), the intelligent card reading and writing module (8) and the audio module (9) are fixedly connected with the shell (1), and the photoelectric module (11) is electrically connected with the control mainboard (5) in a two-way mode.
5. The fluoroimmunoassay quantitative analyzer of claim 3, wherein: the control main board (5) is fixedly connected with the shell (1), and the electric telescopic rod (13) and the screw motor (15) are electrically connected with the control main board (5).
6. The fluoroimmunoassay quantitative analyzer of claim 1, wherein: one side that reagent piece detection groove (20) is close to ultraviolet lamp (16) is provided with isolation glass (21), and isolation glass (21) and reagent piece detection groove (20) sealing connection, one side that reagent piece detection groove (20) is close to reagent piece groove (4) is provided with baffle (22), the upper end of baffle (22) is connected through pivot and casing (1) rotation.
7. The fluoroimmunoassay quantitative analyzer of claim 6, wherein: a spring piece (23) is arranged between the upper end of the baffle plate (22) and the inner wall of the reagent plate detection groove (20), one end of the spring piece (23) is fixedly connected with the upper end of the baffle plate (22), and the other end of the spring piece is fixedly connected with the inner wall of the reagent plate detection groove (20).
8. The fluoroimmunoassay quantitative analyzer of claim 3, wherein: the printing module (6) and the ID reading slot (3) are electrically connected with the control mainboard (5), and the output end of the control mainboard (5) is electrically connected with the input end of the display screen (2).
CN202011018330.5A 2020-09-24 2020-09-24 Fluorescence immunoassay quantitative analyzer Active CN111929431B (en)

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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112881356B (en) * 2021-01-18 2022-06-28 上海雄图生物科技有限公司 High-flux fluorescence immunoassay quantitative POCT analysis device
CN116280627A (en) * 2023-01-16 2023-06-23 哈尔滨商业大学 Reagent kits with detection card reading function

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CN105303685A (en) * 2015-11-10 2016-02-03 昆山古鳌电子机械有限公司 Paper processing device with prompting function
CN206648858U (en) * 2017-04-13 2017-11-17 昆明和合医学检验所有限公司 A kind of portable urine capture and detection means
CN108375674A (en) * 2018-02-27 2018-08-07 李福军 A kind of clinical laboratory's blood analyser
CN108645800A (en) * 2018-05-30 2018-10-12 福州立农环保科技有限公司 A kind of novel water quality detecting device
CN209673807U (en) * 2019-03-05 2019-11-22 郑州科蒂亚生物技术有限公司 A kind of Portable fluorescence immunity analysis instrument
CN210322925U (en) * 2019-03-07 2020-04-14 山西华澈天朗环保科技有限公司 Portable anti-blocking environment detection device
CN210514259U (en) * 2019-09-10 2020-05-12 山东省源丰环保科技有限公司 Air environment detection device

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105303685A (en) * 2015-11-10 2016-02-03 昆山古鳌电子机械有限公司 Paper processing device with prompting function
CN206648858U (en) * 2017-04-13 2017-11-17 昆明和合医学检验所有限公司 A kind of portable urine capture and detection means
CN108375674A (en) * 2018-02-27 2018-08-07 李福军 A kind of clinical laboratory's blood analyser
CN108645800A (en) * 2018-05-30 2018-10-12 福州立农环保科技有限公司 A kind of novel water quality detecting device
CN209673807U (en) * 2019-03-05 2019-11-22 郑州科蒂亚生物技术有限公司 A kind of Portable fluorescence immunity analysis instrument
CN210322925U (en) * 2019-03-07 2020-04-14 山西华澈天朗环保科技有限公司 Portable anti-blocking environment detection device
CN210514259U (en) * 2019-09-10 2020-05-12 山东省源丰环保科技有限公司 Air environment detection device

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