CN111903701A - A kind of veterinary fly-killing premix and its preparation method and application - Google Patents

Abstract

The invention discloses a veterinary fly-killing premix and a preparation method and application thereof. Based on the total weight of the veterinary fly-killing premix, the veterinary fly-killing premix comprises: diflubenzuron 5- 10wt%, EM bacteria powder 0.5-1.0wt%, diluent 10-30wt%, flavoring agent 2-7wt%, carrier 40-70wt%, binder 5-10wt%, wetting agent 1-5wt% and lubricant agent 2 to 8wt%. The premix prepared by the invention has stable curative effect, good medicinal effect, high uniformity and good stability.

Description

A kind of veterinary fly-killing premix and its preparation method and application

technical field

The invention belongs to the technical field of insecticides, and more particularly, relates to a veterinary fly-killing premix and its preparation method and application.

Background technique

People have higher and higher requirements for the livestock and poultry breeding environment, but flies and fly maggots have always been a major problem that plagued farms. Flies are one of the four pests. They not only pollute the environment, but also are carriers of viruses and bacteria. Many human diseases are directly related to flies such as flies. In farms, the prevention and control of flies is a arduous and complicated task, and drug control is an important means, but most of the flyicides are highly toxic, and the residual drugs after use cause serious environmental pollution and even change the ecology. system. Moreover, most flyicides are harmful to human body and seriously threaten human health.

At present, the drugs used to control fly maggots in livestock and poultry manure mainly include traditional insecticides such as organophosphorus, amitamidine and pyrethroid insecticides, as well as the insect growth regulator cyromazine. However, the use of traditional pesticides to control fly maggots in livestock and poultry manure has some drawbacks: for example, organophosphorus pesticides and amitimidine are highly toxic, with high drug residues, and there is a risk of poisoning when livestock and poultry manure is reused; pyrethroids The relative toxicity of the drug is low, but the residual effect is poor and the drug resistance of fly maggots develops quickly. The only special anti-fly maggot drug in veterinary medicine is the insect growth regulator cyromazine. Although the effect of killing flies is very good, it has been used for decades. As the use of cyromazine is getting longer and longer , it is very likely that cyromazine-resistant maggots will appear, which will inevitably lead to an increasing amount of cyromazine added to the feed. Residues of melamine, a metabolite of cyromazine, also increased. At present, Japan and Europe have banned the addition of cyromazine in the feed, and it is urgent to find an anti-fly maggot drug that can be used alternately with cyromazine, is safe for humans and animals, has low toxicity, and is environmentally friendly.

SUMMARY OF THE INVENTION

The object of the present invention is to overcome the defects of the prior art, and to provide a novel veterinary fly-killing premix and a preparation method thereof. The mixture has a good effect of killing flies and maggots, and has high efficiency and low toxicity.

In order to achieve the above object, a first aspect of the present invention provides a veterinary fly-killing premix, which, based on the total weight of the veterinary fly-killing premix, comprises: diflubenzuron 5～10wt%, EM fungus powder 0.5～1.0wt%, diluent 10～30wt%, flavoring agent 2～7wt%, carrier 40～70wt%, binder 5～10wt%, wetting agent 1～5wt% and lubricant 2-8wt%.

In the present invention, EM bacteria is a kind of microbial bacteria preparation composed of more than 80 microorganisms in 10 genera, mainly including photosynthetic bacteria, lactic acid bacteria, yeast bacteria and actinomycetes, generally including photosynthetic bacteria, yeast bacteria, lactic acid bacteria, etc. Beneficial bacteria. It can be used for food addition, breeding disease control, soil improvement, rooting and strong seedlings, sewage treatment and so on.

Diflubenzuron is a specific low-toxicity insecticide, belonging to the benzoic acid-based phenylurea class of insecticides. It has high safety, and the oral LD ₅₀ of rats is more than 5000mg/kg. Its insecticidal mechanism is completely different from that of conventional insecticides in the past. It is neither a nerve agent nor a cholinesterase inhibitor. Its main function is to inhibit the synthesis of chitin in the epidermis of insects, and it also affects the fat body and pharyngeal body. And other endocrine and glands have damage and destruction, thus hindering the smooth molting of insects metamorphosis. The main mode of action is stomach poisoning and contact killing. Accumulated poisoning caused by insects feeding. Due to the lack of chitin, larvae cannot form new epidermis, molting is difficult, and pupation is blocked; adults are difficult to emerge and lay eggs; eggs cannot develop normally, and the hatched larvae's epidermis lack hardness and die, thus It affects the entire generation of pests, which is the advantage of diflubenzuron.

In the veterinary fly-killing premix of the present invention, EM bacteria powder and diflubenzuron are used together, and the two play a role together. Adding a certain amount of EM bacteria powder while using diflubenzuron can greatly improve the control of flies. The effect of diflubenzuron can reduce the impact of the use of diflubenzuron on the animal body and the environment, which greatly improves the fly control effect of the flyicide, effectively reduces the use of diflubenzuron, reduces drug pollution and residues, and has Enhance animal immunity, disease resistance, improve animal health, effectively control and prevent dysentery, coccidia, Escherichia coli and respiratory diseases, promote animal growth, increase daily weight gain, shorten feeding time, reduce or even eliminate antibiotics, and produce green The advantages of livestock and poultry products have broad application prospects in the animal and plant breeding industry.

Premix is a kind of formulation that is convenient for clinical application and easy to be used by livestock workers. It is especially suitable for the preventive treatment of whole animals. If the pesticide is made into premix and added directly to the feed, it will not only save time It is labor-saving and can avoid the stress caused to the animal when the drug is administered.

In veterinary drug premixes, the safety and efficacy of the drug are closely related to the homogeneity. The homogeneity of the premix product is an important factor to ensure product quality, and the homogeneity of the product is closely related to the selection and preparation of excipients. In addition to excipients, acting as carriers, and improving stability, excipients are additions to pharmaceutical preparations. They also have important functions such as solubilization, solubilization, and sustained and controlled release. Adverse reactions have a great relationship, and the selection of suitable excipients is not only related to the homogeneity, fluidity and stability of the premix, but also to the safety of the premix and the efficacy of the drug.

According to the present invention, preferably, the diluent is microcrystalline cellulose and/or lactose.

In general, the diluent is used to fill the weight and volume, and can also play the role of a binder at the same time; the specific diluent used in the present invention can ensure that the fluidity of the premixed material is moderate, and if it is too smooth, it cannot be adsorbed on the carrier. , too rough will lead to uneven dispersion.

According to the present invention, preferably, the flavoring agent is an organic acid and/or aspartame, and the organic acid is preferably citric acid.

On the one hand, the flavoring agent of the present invention is the medicinal adjuvant in order to improve or shield the peculiar smell of medicine, and on the other hand, the dissolubility of diflubenzuron can be increased to a certain extent by adopting the organic acid flavoring agent.

According to the present invention, preferably, the carrier is corn meal and calcium carbonate, and the weight ratio of the corn meal and calcium carbonate is 1-3:1.

The carrier refers to the feedable material that can receive and carry the active ingredient, and the carrier of the present invention can play the role of dilution and improve the fluidity, so that the active ingredient can be easily distributed into the feed.

According to the present invention, preferably, the binder is sodium carboxymethyl cellulose and/or polyvinyl alcohol.

Binders are viscous solid powders or viscous liquids that aggregate and bond non-viscous or low-viscosity materials into granules or compression molding. Due to the poor solubility of diflubenzuron, it cannot be used after being dissolved in water, which limits the way of administration. In the present invention, by adding binder and other auxiliary materials, the diflubenzuron premix can be mixed into feed for administration and also can be mixed into drinking water for administration.

According to the present invention, preferably, the wetting agent is polysorbate 80 and/or absolute ethanol.

According to the present invention, preferably, the lubricant is magnesium stearate.

A second aspect of the present invention provides the preparation method of the above-mentioned veterinary fly-killing premix, the preparation method comprising:

(1) Diflubenzuron, EM bacteria powder, diluent, flavoring agent, carrier, adhesive and lubricant are respectively ground and sieved;

(2) diflubenzuron and the diluent after described grinding, sieving are pulverized and mixed to obtain the first mixed powder;

(3) the first mixed powder is pulverized and mixed with the flavoring agent after the grinding and sieving to obtain the second mixed powder;

(4) pulverizing and mixing the second mixed powder and the ground and sieved carrier to obtain a third mixed powder;

(5) the third mixed powder and the EM bacteria powder are mixed uniformly according to the equal increment method, and then mixed uniformly with the wetting agent, the lubricant and the binder after the grinding and sieving to obtain the pre- mixture.

In the process of mixing the active ingredients and excipients of veterinary drug premixes, on the one hand, the stirring machine plays a role in mixing the materials; There must also be automatic grading, which destroys the uniform state of the material. Therefore, the present invention further limits the mixing method and mixing sequence of the material, which greatly improves the uniformity, stability and safety of the mixed material. sex.

According to the present invention, preferably, in step (1), the time of described grinding is 5-10min, and the particle diameter after described grinding and sieving is not more than 80 meshes;

In step (2), the pulverization and mixing are carried out in a jet mill, the feed speed of the jet mill is 3.0-5.0kg/h, and the air pressure is 2.5×10 ⁵ -4.5×10 ⁵ Pa; The particle size of the mixed powder is D90≤30μm;

In step (3), described pulverization and mixing are carried out in a ball mill, and the time of described pulverization and mixing is 10-15min;

In step (4), the pulverization and mixing are carried out in a ball mill, and the time for pulverization and mixing is 15-25min;

In step (5), mixing is performed in a granulator.

In the production process of premix, the preparation process directly affects the uniformity of the product. Not only accurate ingredients are required, but also effective ingredients must be uniformly distributed in the whole batch of premix. Equipment, material properties, feeding sequence, mixing Time is an important factor.

The third aspect of the present invention provides the application of the above-mentioned veterinary fly-killing premix in animal feed.

The technical scheme of the present invention has the following beneficial effects:

(1) Premix of the present invention contains EM bacterium powder and diflubenzuron, and it uses EM bacterium powder and diflubenzuron in combination, and both play a role together, and add a certain amount of EM bacterium powder while using diflubenzuron It can greatly improve the effect of fly control and fly control, and can reduce the impact of the use of diflubenzuron on the animal body and the environment, which greatly improves the fly control effect of the fly control agent, effectively reduces the use of diflubenzuron, and reduces the use of drugs. Pollution and residues, but also enhance animal immunity, disease resistance, improve animal health, effectively control and prevent dysentery, coccidia, Escherichia coli and respiratory diseases, promote animal growth, increase daily weight gain, shorten feeding time, reduce , Even without antibiotics, the advantages of producing green livestock and poultry products have broad application prospects in the animal and plant breeding industry.

(2) binder and organic acid are added in the premix of the present invention, and the solubility of diflubenzuron is increased.

(3) In the pretreatment process of the preparation method of the present invention, the conditions are mild and belong to physical changes, and there is no chemical reaction, and the yield of diflubenzuron can be maintained at 100% without any loss.

(4) The micronization technology is adopted in the preparation process of the premix of the present invention, the particle size of diflubenzuron is reduced, and the solubility of diflubenzuron is increased, so that the premix of diflubenzuron can be mixed into the feed for administration, and also Can be mixed with drinking water for administration.

(5) The production and preparation process of the present invention is simple and feasible, the quality is controllable, the operation is simple, the cost is low, and the pollution is small.

(6) The premix prepared by the present invention has stable curative effect, good efficacy, high uniformity and good stability.

Other features and advantages of the present invention will be described in detail in the detailed description that follows.

Detailed ways

Preferred embodiments of the present invention will be described in more detail below. While preferred embodiments of the present invention are described below, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.

The EM bacteria powder used in each example and the comparative example was purchased from Cangzhou Dongfang Veterinary Medicine Co., Ltd.

Example 1

This embodiment provides a veterinary fly-killing premix, which is composed of the following components: diflubenzuron 67g, EM bacterial powder 6.7g, microcrystalline cellulose 200g, citric acid 50g, Corn flour 300g, calcium carbonate 226.3g, sodium carboxymethyl cellulose 75g, polysorbate 80 25g and magnesium stearate 50g;

The specific preparation method is: (1) Diflubenzuron, EM bacterial powder, microcrystalline cellulose, citric acid, corn flour, calcium carbonate, sodium carboxymethyl cellulose and magnesium stearate of the recipe quantity are respectively ground for 10min After passing through an 80-mesh sieve, it is ready for use;

(2) The diflubenzuron and microcrystalline cellulose after grinding and sieving are input into the jet mill through dry nitrogen (the feed rate of the jet mill is 5.0kg/h, the air pressure is 4.5×10 ⁵ Pa), and the pellets are obtained The first mixed powder with diameter D90≤30μm;

(3) the citric acid after grinding, sieving is added in the described first mixed powder and pulverized and mixed 15min in a ball mill to obtain the second mixed powder;

(4) the corn flour after grinding, sieving and calcium carbonate are added to the second mixed powder and pulverized and mixed 25min in a ball mill to obtain the third mixed powder;

5) The third mixed powder and EM bacteria powder are dropped into the granulator according to the equal increment method and mixed, add the magnesium stearate and sodium carboxymethyl cellulose after grinding and sieving, and spray into the polysorbate After ester 80, mix evenly to obtain veterinary fly-killing premix.

Example 2

This embodiment provides a veterinary fly-killing premix, which is composed of the following components: diflubenzuron 67g, EM bacterial powder 6.7g, microcrystalline cellulose 250g, citric acid 50g, 250g corn flour, 226.3g calcium carbonate, 75g sodium carboxymethyl cellulose, 25g absolute ethanol and 50g magnesium stearate;

The specific preparation method is: (1) Diflubenzuron, EM bacterial powder, microcrystalline cellulose, citric acid, corn flour, calcium carbonate, sodium carboxymethyl cellulose and magnesium stearate of the recipe quantity are respectively ground for 10min After passing through an 80-mesh sieve, it is ready for use;

(2) The diflubenzuron and microcrystalline cellulose after grinding and sieving are input into the jet mill through dry nitrogen (the feed rate of the jet mill is 5.0kg/h, the air pressure is 4.5×10 ⁵ Pa), and the pellets are obtained The first mixed powder with diameter D90≤30μm;

(3) the citric acid after grinding, sieving is added in the described first mixed powder and pulverized and mixed 15min in a ball mill to obtain the second mixed powder;

(4) the corn flour after grinding, sieving and calcium carbonate are added to the second mixed powder and pulverized and mixed 25min in a ball mill to obtain the third mixed powder;

5) The third mixed powder and EM bacteria powder are dropped into the granulator according to the equal increment method and mixed, add the magnesium stearate and sodium carboxymethyl cellulose after grinding and sieving, and spray into dehydrated alcohol After mixing evenly, the veterinary fly-killing premix is obtained.

Example 3

This embodiment provides a veterinary fly-killing premix, which is composed of the following components: diflubenzuron 67g, EM bacterial powder 6.7g, microcrystalline cellulose 200g, aspartame 50g , corn flour 300g, calcium carbonate 226.3g, sodium carboxymethyl cellulose 75g, polysorbate 80 25g and magnesium stearate 50g;

The concrete preparation method is as follows: (1) Diflubenzuron, EM bacterial powder, microcrystalline cellulose, aspartame, corn flour, calcium carbonate, sodium carboxymethyl cellulose and magnesium stearate of recipe quantity are ground respectively After 10 minutes, pass through an 80-mesh sieve for use;

(2) The diflubenzuron and microcrystalline cellulose after grinding and sieving are input into the jet mill through dry nitrogen (the feed rate of the jet mill is 5.0kg/h, the air pressure is 4.5×10 ⁵ Pa), and the pellets are obtained The first mixed powder with diameter D90≤30μm;

(3) the aspartame after grinding, sieving is added in the described first mixed powder and pulverized and mixed 15min in a ball mill to obtain the second mixed powder;

(4) the corn flour after grinding, sieving and calcium carbonate are added to the second mixed powder and pulverized and mixed 25min in a ball mill to obtain the third mixed powder;

5) The third mixed powder and EM bacteria powder are dropped into the granulator according to the equal increment method and mixed, add the magnesium stearate and sodium carboxymethyl cellulose after grinding and sieving, and spray into the polysorbate After ester 80, mix evenly to obtain veterinary fly-killing premix.

Example 4

This embodiment provides a veterinary fly-killing premix, which is composed of the following components: diflubenzuron 67g, EM bacteria powder 6.7g, lactose 200g, citric acid 50g, corn flour 300g , calcium carbonate 226.3g, sodium carboxymethyl cellulose 75g, polysorbate 80 25g and magnesium stearate 50g;

The concrete preparation method is: (1) Diflubenzuron, EM bacterial powder, lactose, citric acid, corn flour, calcium carbonate, sodium carboxymethyl cellulose and magnesium stearate of the recipe quantity are respectively ground for 10min and passed 80 minutes. Mesh sieve spare;

(2) The diflubenzuron and lactose after grinding and sieving are input into the jet mill through dry nitrogen (the feed rate of the jet mill is 5.0kg/h, the air pressure is 4.5×10 ⁵ Pa), and the particle size D90≤ 30μm first mixed powder;

(3) the citric acid after grinding, sieving is added in the described first mixed powder and pulverized and mixed 15min in a ball mill to obtain the second mixed powder;

(4) the corn flour after grinding, sieving and calcium carbonate are added to the second mixed powder and pulverized and mixed 25min in a ball mill to obtain the third mixed powder;

5) The third mixed powder and EM bacteria powder are dropped into the granulator according to the equal increment method and mixed, add the magnesium stearate and sodium carboxymethyl cellulose after grinding and sieving, and spray into the polysorbate After ester 80, mix evenly to obtain veterinary fly-killing premix.

Example 5

This embodiment provides a veterinary fly-killing premix, which is composed of the following components: diflubenzuron 67g, EM bacterial powder 6.7g, microcrystalline cellulose 200g, citric acid 50g, Corn flour 300g, calcium carbonate 226.3g, polyvinyl alcohol 75g, polysorbate 80 25g and magnesium stearate 50g;

The concrete preparation method is: (1) Diflubenzuron, EM bacterial powder, microcrystalline cellulose, citric acid, corn flour, calcium carbonate, polyvinyl alcohol and magnesium stearate of the recipe quantity are respectively ground for 10min and then pass through 80 meshes. sieve spare;

(2) The diflubenzuron and microcrystalline cellulose after grinding and sieving are input into the jet mill through dry nitrogen (the feed rate of the jet mill is 5.0kg/h, the air pressure is 4.5×10 ⁵ Pa), and the pellets are obtained The first mixed powder with diameter D90≤30μm;

(3) the citric acid after grinding, sieving is added in the described first mixed powder and pulverized and mixed 15min in a ball mill to obtain the second mixed powder;

(4) the corn flour after grinding, sieving and calcium carbonate are added to the second mixed powder and pulverized and mixed 25min in a ball mill to obtain the third mixed powder;

5) The third mixed powder and EM bacteria powder are dropped into the granulator according to the equal increment method and mixed, add the magnesium stearate and polyvinyl alcohol after grinding and sieving, and spray into the polysorbate 80 after Mix evenly to obtain veterinary fly control premix.

Example 6

This embodiment provides a veterinary fly-killing premix, which is composed of the following components: diflubenzuron 67g, EM bacterial powder 6.7g, microcrystalline cellulose 200g, citric acid 50g, Corn flour 300g, calcium carbonate 226.3g, polyvinyl alcohol 75g, anhydrous ethanol 25g and magnesium stearate 50g;

The concrete preparation method is: (1) Diflubenzuron, EM bacteria powder, microcrystalline cellulose, citric acid, corn flour, calcium carbonate, polyvinyl alcohol and magnesium stearate of the recipe quantity are respectively ground for 7min to pass 80 meshes. sieve spare;

(2) The diflubenzuron and microcrystalline cellulose after grinding and sieving are input into the jet mill through dry nitrogen (the feed speed of the jet mill is 4.0kg/h, the air pressure is 3.0×10 ⁵ Pa), and the pellets are obtained The first mixed powder with diameter D90≤30μm;

(3) the citric acid after grinding, sieving is added in the described first mixed powder and pulverized and mixed 7min in a ball mill to obtain the second mixed powder;

(4) corn flour after grinding, sieving and calcium carbonate are added to the second mixed powder and pulverized and mixed 20min in a ball mill to obtain the third mixed powder;

5) The third mixed powder and EM bacteria powder are dropped into the granulator according to the equal increment method and mixed, add the magnesium stearate and polyvinyl alcohol after grinding and sieving, and mix after being sprayed into dehydrated alcohol. , that is, veterinary fly control premix.

Comparative Example 1

The formula of the premix of this comparative example is the same as that of Example 1, and the difference is only the preparation method. The preparation method of this comparative example is: diflubenzuron, EM bacteria powder, microcrystalline cellulose, citric acid, corn flour , calcium carbonate, sodium carboxymethyl cellulose and magnesium stearate are respectively sieved with 80 meshes, then the above-mentioned components after sieving are mixed with polysorbate 80 to obtain a premix.

Comparative Example 2

The difference between this comparative example and Example 1 is only to remove the EM bacteria powder of 6.7g, and the consumption of diflubenzuron is adjusted to 73.7g, and others are the same as in Example 1.

Comparative Example 3

The difference between this comparative example and Example 1 is only that the EM bacteria powder of 6.7g and the diflubenzuron of 67g are replaced by the cyromazine of 73.7g, and others are all the same as in Example 1.

Test Example 1

According to "Chinese Pharmacopoeia" 2015 edition appendix granules (granules) general rule--0104 quality requirements of granules, the premixes prepared in Examples 1-6 of the present invention and Comparative Examples 1-2 were respectively tested for appearance, hardness, The granulation rate and content are tested, and the results are shown in Table 1 below;

Table 1 Quality test results of different premixes

group Exterior hardness Granulation rate (%) Indicated content (%) Example 1 meet the requirements meet the requirements 90 101.5 Example 2 meet the requirements meet the requirements 89 100.7 Example 3 meet the requirements meet the requirements 90 100.2 Example 4 meet the requirements meet the requirements 88 99.8 Example 5 meet the requirements meet the requirements 89 101.8 Example 6 meet the requirements meet the requirements 90 99.6 Comparative Example 1 local agglomeration have broken 72 99.1

As can be seen from Table 1, the contents of the premixes prepared in Examples 1-6 of the present invention all meet the quality requirements, and the contents are 99.0 to 102.0% of the indicated amount. The appearance, hardness and granulation rate of the premixes prepared in Examples 1-6 all meet the requirements, and the granulation rate of the premixes prepared in Example 1, Example 3 and Example 6 is the highest, all reaching 90% . In Comparative Example 1, the granulation rate decreased significantly because of poor mixing.

Test case 2

1. Test materials: the premix prepared in Example 1; the premix prepared in Example 2; the premix prepared in Comparative Example 2; the premix prepared in Comparative Example 3.

2. Test method: Select 100 commercial pigs from a pig farm and divide them into 5 groups, with 20 pigs each as a group. Group A is the premix feeding group prepared in Example 1, Group B is the premix feeding group prepared in Example 2, Group C is the premix feeding group prepared in Comparative Example 2, and Group D is the comparative example 3 The prepared premix feeding group and E group were blank control group. Animal grouping and treatment are shown in Table 2 below.

Table 2 Grouping and treatment of experimental animals

group Quantity (head) deal with Group A 20 Add 30g of the premix prepared in Example 1 per 1000Kg of feed to feed Group B 20 Add 30g of the premix prepared in Example 2 per 1000Kg of feed to feed Group C 20 Add 30g of the premix prepared in Comparative Example 2 to feed per 1000Kg of feed Group D 20 Add 30g of the premix prepared in Comparative Example 3 to feed per 1000Kg of feed Group E 20 No drugs are added to the feed

Group A, Group B, Group C and Group D were fed the premix prepared in Example 1, the premix prepared in Example 2, the premix prepared in Comparative Example 2, and the premix prepared in Comparative Example 3 from the sixth day, respectively. premix for 7 consecutive days.

Pig feces were collected in each group on the 1st, 3rd, and 5th days (drug-free), 6th, 8th, 10th, and 12th days (during drug use, 13th, 15th, 17th, and 19th days (after drug withdrawal). The collection points should be evenly distributed. Mix the feces from different sampling points in each group and mix well. The total sampling volume of each group is 1L.

3. Evaluation method Divide each sample into 6 parts and put them into 6 200ml beakers respectively.

The mouth of the cup was closed with gauze, and cultured in an environment with a temperature of 25° C. and a humidity of about 60% for 3 weeks. During this period, live and dead fly maggots were counted, and the mean mortality and standard deviation of the fly maggots in the 6 beakers per sample were calculated.

4. Results and discussion

The average mortality of fly maggots in different treatments of pig manure is shown in Table 3. Statistical analysis of variance was performed on these data.

Table 3 Mortality (%) of fly maggots in feces

1 day 3 days 5 days 6 days 8 days 10 days 12 days 13 days 15 days 17 days 19 days Group A 30a 50a 75a 100a 100a 100a 100a 100a 100c 75a 50a Group B 30a 50a 50a 100a 100a 100a 100a 100a 100c 75a 30a Group C 20a 30a 50a 100a 100a 100a 100a 100a 75c 30a 15a Group D 10a 25a 50a 100a 100a 100a 100a 100a 25a 10a 5a Group E 5a 5a 2a 10b 20b 5b 25b 5b 5a 5a 5a

Note: If the lowercase letters of each column of test data in groups A-E are the same (all a, b or c), the difference is not significant (P>0.05), and if the lowercase letters are different, the difference is significant (P<0.05).

The results showed that the test groups of group A and group B showed 100% death in pig feces from the day after administration to 3 days after drug withdrawal, and from the day after administration to 1 day after drug withdrawal in groups C and D. There was a significant difference between it and the blank control group (P<0.05).

Compared with group A, group B, group C and group D for 7 days, they have the same clinical effect of controlling fly maggots, and the difference is not significant (P>0.05). Group A and B had longer duration of drug effect than group C and D. Therefore, feeding the premix containing EM bacteria powder and diflubenzuron had the best effect. Moreover, it is more convenient and economical to use the premix of the present invention. More importantly, feeding cyromazine to pigs has the risk of residual melamine metabolite, but the use of the premix containing EM bacteria powder and diflubenzuron according to the present invention can completely solve the residual melamine in foods such as pork. The problem.

Test case 3

This test example tests the effect of the premix of the present invention on pig feed intake.

Select 8 pens of weaned piglets raised in the same nursery, with 30 pigs in each pen, and randomly divide them into 8 groups, which are blank group, control group and feeding group for animal feed intake test: the daily diet of blank group is Common nursery pig feed, the control group is the premix prepared by adding about 500ppm in the common nursery pig feed in Comparative Example 2, and the feeding group adds about 500ppm in the common nursery pig feed The product prepared by the present invention contains EM bacteria powder and diflubenzuron premix. Wherein the feeding groups 1-6 are respectively the premix containing EM fungus powder and diflubenzuron prepared according to the method described in Examples 1-6 of the present invention.

The pig house was sterilized according to the routine, and the pigs were raised on a high bed with slats so that they could eat and drink freely. Continue feeding for 14 days according to the above-mentioned scheme, and before morning feeding for 14 days, carry out fasting weighing, and calculate daily weight gain. In this test, the common nursery pig feed is based on the total weight of the common nursery pig feed, and the common nursery pig feed is composed of the following components: 65wt% corn, 8wt% puffed soybean, 4wt% fish meal, 5wt% whey powder, milk replacer powder 4wt%, fermented soybean meal 4wt%, soybean meal 4wt%, premix 6wt%.

The influence of table 4 premix of the present invention on pig feed intake

Initial weight (kg) Final weight (kg) Average feed intake (g/d) Daily weight gain (g/d) blank group 5.92 soil 0.52 7.48 soil 0.58 285.26 soil 24.61 110.42 soil 13.58 control group 5.65 soil 0.74 8.18 soil 0.77 192.30 soil 35.69 179.79 soil 40.74 Feeding group 1 5.84 soil 0.70 8.87 soil 0.53 344.43 soil 19.52 305.03 soil 17.51 Feeding group 2 5.93 soil 0.62 9.25 soil 0.74 267.41 soil 40.66 236.03 soil 35.33 Feeding group 3 5.98 soil 1.20 8.92 soil 1.00 230.84 soil 40.41 208.56 soil 37.43 Feeding group 4 5.76 soil 0.44 8.71 soil 0.76 244.86 soil 34.82 209.26 soil 24.61 Feeding group 5 5.85 soil 0.58 9.31 soil 0.95 250.38 soil 36.62 246.35 soil 15.75 Feeding group 6 5.98 soil 1.04 9.17 soil 1.15 238.58 soil 40.69 223.67 soil 23.16

In Table 4, the initial weight is the weight before this feeding test is performed, and the final weight is the weight measured on an empty stomach before morning feeding for 14 days.

Compared with the blank group and the control group, the average feed intake of the feeding group is greatly improved, so that the daily gain is also significantly improved, and the data has a significant difference, and the feeding group 1 is the best embodiment of the present invention.

Various embodiments of the present invention have been described above, and the foregoing descriptions are exemplary, not exhaustive, and not limiting of the disclosed embodiments. Numerous modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the described embodiments.

Claims (10)

1. a veterinary fly-killing premix is characterized in that, in the gross weight of described veterinary fly-killing premix, this veterinary fly-killing premix comprises: diflubenzuron 5～10wt%, EM Bacteria powder 0.5-1.0wt%, diluent 10-30wt%, flavoring agent 2-7wt%, carrier 40-70wt%, binder 5-10wt%, wetting agent 1-5wt% and lubricant 2-8wt% %. 2. The veterinary fly-killing premix according to claim 1, wherein the diluent is microcrystalline cellulose and/or lactose. 3. The veterinary fly-killing premix according to claim 1, wherein the flavoring agent is an organic acid and/or aspartame, and the organic acid is preferably citric acid. 4. veterinary fly-killing premix according to claim 1, wherein, described carrier is corn meal and calcium carbonate, and the weight ratio of described corn meal and calcium carbonate is 1-3:1. 5. The veterinary fly-killing premix according to claim 1, wherein the binder is sodium carboxymethyl cellulose and/or polyvinyl alcohol. 6. The veterinary fly-killing premix according to claim 1, wherein the wetting agent is polysorbate 80 and/or absolute ethanol. 7. The veterinary fly-killing premix according to claim 1, wherein the lubricant is magnesium stearate. 8. the preparation method of the veterinary fly-killing premix described in any one in claim 1-7, is characterized in that, this preparation method comprises: (1) Diflubenzuron, EM bacteria powder, diluent, flavoring agent, carrier, adhesive and lubricant are respectively ground and sieved; (2) diflubenzuron and the diluent after described grinding and sieving are pulverized and mixed to obtain the first mixed powder; (3) pulverizing and mixing the first mixed powder and the flavoring agent after the grinding and sieving to obtain the second mixed powder; (4) pulverizing and mixing the second mixed powder and the ground and sieved carrier to obtain a third mixed powder; (5) the third mixed powder and the EM bacteria powder are mixed uniformly according to the equal increment method, and then mixed uniformly with the wetting agent, the lubricant and the binder after the grinding and sieving to obtain the pre- mixture. 9. preparation method according to claim 1, wherein, in step (1), the time of described grinding is 5-10min, and the particle diameter after described grinding and sieving is not more than 80 meshes; In step (2), the pulverization and mixing are carried out in a jet mill, the feed speed of the jet mill is 3.0-5.0kg/h, and the air pressure is 2.5×10 ⁵ -4.5×10 ⁵ Pa; The particle size of the mixed powder is D90≤30μm; In step (3), the pulverization and mixing are carried out in a ball mill, and the time for pulverization and mixing is 10-15min; In step (4), the pulverizing and mixing are carried out in a ball mill, and the time for pulverizing and mixing is 15-25 min; In step (5), mixing is performed in a granulator. 10. The application of the veterinary fly-killing premix described in any one of claims 1-7 in animal feed.