CN111887895A - A saliva collection device with a disease-enriching adsorption structure - Google Patents

Abstract

The invention provides a saliva collecting device with a disease-enriching adsorption structure, comprising a sampler and a sample storage bottle; the sampler includes a hollow package, a resin adsorption module and a negative pressure control device; the package is provided with a collection port and a negative pressure The resin adsorption module is placed in the package, and is located at the end of the package close to the collection port; the negative pressure control device is detachably connected to the negative pressure port of the package; the sample storage bottle is preset with a sample preservation solution; after sampling by the sampler , inserted in the storage bottle, to propose a saliva collection device that realizes effective adsorption and enrichment for viruses and realizes short-term preservation of viruses.

Description

A saliva collection device with a disease-enriching adsorption structure

technical field

The invention relates to the technical field of biological preservation, in particular to a saliva collecting device with a disease-enriching adsorption structure.

Background technique

Saliva is an important biological fluid and one of the commonly used test specimens in biomedical research and clinical practice. Under normal physiological conditions, in addition to the oral epithelial cells exfoliated from the donor's mouth, saliva also contains a variety of protein/enzyme molecules, extracellular vesicles/exosomes, and micronucleic acid molecules secreted from salivary glands. Microbial cells of normal flora, etc. In disease states such as viral or bacterial infections, it may also contain viral particles, bacterial or fungal pathogen cells. Studies have shown that saliva samples are more stable and reliable than samples collected by other methods such as nasopharyngeal swabs for viral nucleic acid detection after respiratory virus infection. Various existing devices for saliva collection and sampling are simple and direct, without adsorption and enrichment for the biological components to be detected, no non-toxic built-in signs of successful sampling, and no protection against related biological components; Sampling operations must also be performed by trained professionals. Therefore, the existing saliva sampling device cannot be completed by the general population itself, nor can it be used for short-term preservation of samples, so it cannot be used for remote delivery of saliva samples.

SUMMARY OF THE INVENTION

The purpose of the present invention is to propose a saliva collection device for realizing effective adsorption and enrichment of viruses and short-term preservation of viruses.

In order to achieve the above object, the present invention proposes a saliva collection device with a disease-enriching adsorption structure, including a sampler and a sample storage bottle;

The sampler includes a hollow package, a resin adsorption module and a negative pressure control device; the package is provided with a collection port and a negative pressure port; the resin adsorption module is placed in the package and is arranged in the package one end close to the collection port; the negative pressure control device is detachably connected to the negative pressure port of the package;

The sample storage bottle is preset with a sample preservation solution; the sampler is inserted into the sample storage bottle after sampling.

Preferably, the package is a hollow glass package, the package has a columnar structure, the first end of the package is the collection port, and the tail end of the package is the negative pressure port.

Preferably, the resin adsorption module is a mixture of ion exchange resin and chitosan or dextran particles with strong adsorption force for viruses and bacteria.

Preferably, the resin adsorption module is a mixture of porous ion exchange resin, sialylated ion exchange resin or/and chitosan;

Preferably, the resin adsorption module is cellulose, dextran or/and agarose gel.

Preferably, the resin adsorption module is arranged in the package in the form of a cylinder, a square cylinder, a sheet or a beaded structure.

Preferably, the negative pressure device is a rubber suction ball, and the rubber suction ball is sleeved on the negative pressure port of the sample storage bottle.

Preferably, the sample storage bottle is a glass bottle, and a bottle cap is screwed and fixed at the bottle mouth of the glass bottle;

The sample preservation solution is a physiological buffer, and the physiological buffer includes serum-free cell culture solution, Hank's buffer, PBS phosphate buffer, TRIS buffer or physiological saline; the physiological buffer is also added with phenol red indicator Antibiotics, compound broad-spectrum antibiotics, including penicillin, streptomycin, and amphotericin B.

Preferably, it also includes a filter paper strip, the filter paper strip is placed in the package, the filter paper strip is connected with the resin adsorption module, and the wet printing mark line is printed on the filter paper strip, so The wet-printed marking lines are engraved with water-colored ink.

Compared with the prior art, the advantage of the present invention lies in that: the resin adsorption module is arranged in the sampler in the saliva collection device of the present invention, which can improve the collection efficiency of pathogens and other biological components to be detected, that is, improve the extraction efficiency. concentration in the sample.

In the process of adopting, it can be judged whether the sampling is sufficient by observing the wet printing mark on the filter paper strip in the sampler, so as to ensure the accuracy of sampling and improve the sampling efficiency.

The operation is simple, and ordinary adults can operate it immediately according to the graphic instructions without professional training;

The sampler is used in combination with the storage bottle, which can facilitate the short-term preservation of samples under normal room temperature conditions, and is suitable for remote delivery, convenient sample transportation and guaranteeing the quality of sampling.

Description of drawings

1 is a schematic structural diagram of a saliva collection device in an embodiment of the present invention;

Fig. 2 is the structural representation of the sampler in the embodiment of the present invention;

FIG. 3 is a flow chart of the use of the sampler and the sample storage bottle in the embodiment of the present invention.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be further described below.

As shown in FIG. 1 , the present invention proposes a saliva collection device with a disease-enriching adsorption structure, comprising a sampler 1 and a sample storage bottle 2;

As shown in FIG. 2, the sampler 1 includes a hollow package 4, a resin adsorption module 3 and a negative pressure control device 5; the package 4 is provided with a collection port and a negative pressure port; the resin adsorption module 3 is placed in the package 4, and is provided with The negative pressure control device 5 is detachably connected to the negative pressure port of the package 4; it also includes a filter paper strip 6, and the filter paper strip 6 is placed in the package 4, and the filter paper strip 6 is adsorbed on the resin. The modules 3 are connected, and the filter paper strip 6 is printed with a wet printing mark line 7, and the wet printing mark line 7 is engraved with water-colored ink.

In this embodiment, the method of using the sampler 1 is as follows: the user generates a negative pressure inside the package 4 by operating the negative pressure control device 5 , and at this time, the virus is adsorbed on the resin adsorption module 3 . When the adsorption of the virus is close to saturation, the water will extend to the filter paper strip 6 along the resin adsorption module 3. When the wet printing mark line 7 on the filter paper strip 6 is wet, it will change color when it encounters water, indicating that the sampler 1 has collected. To the set sufficient amount of saliva sample; wherein, the water-colored ink does not have any damaging effect on the sample.

In this embodiment, the resin adsorption module 3 is a mixture of ion-exchange resin and chitosan or dextran particles solidified with a special virus receptor structure, which has strong adsorption force for various viruses and bacteria; Exchange resin, sialylated ion exchange resin or/and chitosan mixture; resin adsorption module 3 can also be cellulose, dextran or/and agarose gel; it can be selected according to practical application requirements.

In view of the high affinity of the surface groups of ion exchange resin, sialic acid and chitosan molecules to a variety of virus particles, including influenza virus and COVID-19 new coronavirus, during the sampling process, resin adsorption module 3 can capture saliva. Viral particles are trapped within the module, increasing the concentration of viral particles in the collected sample. The affinity capture activity of the adsorption module is also effective against bacterial and fungal microorganisms, exfoliated endothelial cells, and extracellular particles. Therefore, the resin adsorption module 3 will improve the collection efficiency of potential pathogens or other biological components.

The resin adsorption module 3 is a cylinder, a square cylinder, a sheet or a beaded structure and is arranged in the package 4. In this embodiment, the resin adsorption module 3 adopts a multi-layer sheet structure 12, which is convenient for the layered adsorption of viruses. .

In this embodiment, the package 4 is a hollow glass package 4, the package 4 has a columnar structure, the first end of the package 4 is a collection port, and the tail end of the package 4 is a negative pressure port. It is convenient to observe the sample collection situation in the package 4 .

In this embodiment, the sample storage bottle 2 is a glass bottle 8, a bottle cap 10 is screwed and fixed at the bottle mouth of the glass bottle, and a sealing cap 11 is provided at the bottle cap 10 and the bottle mouth; the sample storage bottle 2 is preset with a Sample preservation solution; after sampling by the sampler 1, it is inserted into the sample storage bottle 2.

In the actual application process, after sampling by the sampler 1, it can be directly placed in the storage bottle 2, and the negative pressure control device 5 can be removed for preservation. In this embodiment, the negative pressure control device 5 adopts a rubber suction ball, and the rubber suction ball is sleeved on the negative pressure port of the sample storage bottle 2. The operator squeezes the rubber suction ball to realize the negative pressure inside the sampler 1. Pressed state, so as to achieve the adsorption and removal of virus samples. When the sampler 1 is placed in the storage bottle 2, the rubber suction ball is squeezed at the same time, and part of the saliva sample is discharged into the storage bottle, then the rubber suction ball is removed from the negative pressure port of the sampler 1, and the bottle cap 10 is screwed on. The safe preservation of saliva samples can be achieved, and then before the samples are sent to the laboratory for testing, the samples can be stored for a short period of time to ensure the quality of sample collection. After being sent to the laboratory, the bottle cap 10 can be opened, and the sample can be directly drawn from the upper end opening of the sampler 1. The operation is simple and convenient, and it is safe and greatly reduces the risk of sample contamination or leakage. For some detection equipment that needs a carrier, the sample storage bottle 2 in which the sample is stored can be directly cooperated with the detection equipment to complete the detection.

In this embodiment, the sample preservation solution is a physiological buffer 9, and the physiological buffer 9 includes serum-free cell culture solution, Hank's buffer, PBS phosphate buffer, TRIS buffer or physiological saline; the physiological buffer 9 is also added with Phenol red indicator, compound broad-spectrum antibiotics, including penicillin, streptomycin, and amphotericin B. For the collection of specific virus samples such as COVID-19 novel coronavirus samples, diethylpyrocarbonate (DEPC), an RNase inhibitor that helps stabilize RNA, can also be added to the buffer. The antibiotic helps to inhibit potential microbial growth during short-term storage, and the phenol red indicator helps to indicate whether the pH of the sample buffer has changed during storage.

The above are only preferred embodiments of the present invention, and do not have any limiting effect on the present invention. Any person skilled in the art, within the scope of not departing from the technical solution of the present invention, makes any form of equivalent replacement or modification to the technical solution and technical content disclosed in the present invention, all belong to the technical solution of the present invention. content still falls within the protection scope of the present invention.

Claims (9)

1. A saliva collecting device with a disease enrichment adsorption structure is characterized by comprising a sampler and a sample storage bottle;

the sampler comprises a hollow package, a resin adsorption module and a negative pressure control device; the package is provided with a collection port and a negative pressure port; the resin adsorption module is arranged in the package and is arranged at one end, close to the acquisition port, in the package; the negative pressure control device is detachably connected to the negative pressure port of the package;

a sample preservation solution is preset in the sample storage bottle; and the sampler is inserted into the sample storage bottle after sampling.

2. The saliva collection device with a disease enrichment adsorption structure of claim 1, wherein the package is a hollow glass package, the package is a cylindrical structure, the head end of the package is the collection port, and the tail end of the package is the negative pressure port.

3. The saliva collection device with a disease enrichment adsorption structure of claim 1, wherein the resin adsorption module is a mixture of ion exchange resin and chitosan or dextran particles having strong adsorption force to viruses and bacteria.

4. The saliva collection device with a disease enrichment adsorption structure of claim 3, wherein the resin adsorption module is a porous ion exchange resin, a sialylated ion exchange resin, or/and a mixture of chitosan.

5. The saliva collection device with a disease enrichment adsorption structure of claim 1, wherein the resin adsorption module is cellulose, dextran or/and sepharose.

6. The saliva collection device with a disease enrichment adsorption structure of claim 1, wherein the resin adsorption modules are arranged in the package in a cylinder, a square cylinder, a sheet or a bead-like structure.

7. The saliva collecting device with the disease enrichment adsorption structure as claimed in claim 1, wherein the negative pressure device is a rubber suction ball which is sleeved on the negative pressure port of the sample storage bottle.

8. The saliva collecting device with the disease enrichment adsorption structure as claimed in claim 1, wherein the sample storage bottle is a glass bottle, and a bottle cap is screwed and fixed at the bottle mouth of the glass bottle;

the sample preservation solution is a physiological buffer solution, and the physiological buffer solution comprises serum-free cell culture solution, Hank's buffer solution, PBS phosphate buffer solution, TRIS buffer solution or physiological saline; the physiological buffer solution is also added with phenol red indicator and composite broad spectrum antibiotics, including penicillin, streptomycin and amphotericin B.

9. The saliva collection device with a disease enrichment adsorption structure of claim 1, further comprising a paper filter strip, wherein the paper filter strip is placed in the package and connected with the resin adsorption module, and wet-printed mark lines are printed on the paper filter strip and engraved with water-color ink.

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