CN111876323A - A kind of virus sampling, preservation and detection composite tube and using method thereof - Google Patents

Abstract

The invention discloses a virus preservation and detection composite tube and a use method thereof. The composite pipe includes: the device comprises a pipe body, an upper cover and a lower cover, wherein a main cavity, an exchange cavity and a detection cavity which are communicated with an opening at the upper end of the pipe body are separately arranged in the pipe body, and the main cavity is communicated with the detection cavity through the exchange cavity; the lower cover is provided with a sealing element extending into the exchange cavity, and the lower cover is rotated to drive the sealing element to rotate in the exchange cavity, so that the exchange cavity communicated with the main cavity and the detection cavity is sealed or communicated. The invention has simple and practical structure and simple operation, can reduce the operation links of detection and save the detection cost. The invention can not only realize on-site sampling and on-site rapid detection, but also realize separate sampling and centralized detection, and simultaneously, the main cavity and the detection cavity are relatively sealed, so that the condition of cross contamination can not occur.

Description

A kind of virus sampling, preservation and detection composite tube and using method thereof

Technical field:

The invention relates to the technical field of virus preservation test tube products, in particular to a virus preservation and detection composite tube and a method of using the same.

Background technique:

At present, in the process of collecting and testing some viruses, a separate virus sampling tube or test tube is used for sampling and sealing, and then the detection reagent card or special instrument is used in a special laboratory for detection. This method uses a lot of instruments and the operation procedure is relatively It is complicated and requires specially trained personnel and specialized laboratories for testing, which is costly and inefficient, especially in places where the testing level is not high. In addition, the tested samples of the above detection methods need to be transferred between sampling tubes, detection reagent cards, or detection instruments. During this process, the samples are not in a sealed state, which is very likely to cause secondary transmission of the virus or air pollution. Dangerous. In view of this deficiency, the present invention creatively proposes a composite detection tube that integrates virus sampling, sample preservation and detection.

In view of the above situation, the inventors have made improvements and proposed the following technical solutions.

Invention content:

The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art, and to provide a virus sampling, preservation and detection composite tube capable of storing, collecting, and detecting virus samples at the same time.

In order to solve the above-mentioned technical problems, the present invention adopts the following technical scheme: a composite tube for virus sampling, preservation and detection, comprising: a tube body, an upper cover and a lower cover respectively installed on the upper and lower ends of the tube body, and the A main cavity, an exchange cavity and a detection cavity communicated with the opening of the upper end of the pipe body are separated and arranged, wherein the main cavity and the detection cavity are communicated through the exchange cavity; the lower cover has a protruding into the exchange cavity For the sealing member of the cavity, rotating the lower cover drives the sealing member to rotate in the exchange cavity, thereby realizing the sealing or communication of the exchange cavity connecting the main cavity and the detection cavity.

Further, in the above technical solution, the inner wall of the lower cover is formed with a clamping block, the outer wall of the pipe is provided with an annular flange corresponding to the clamping block, and the clamping block is connected with the annular flange through the buckle. Realize the connection between the lower cover and the tube body.

Further, in the above-mentioned technical solution, a protective pull bar is fixedly connected to the lower cover, and a positioning mechanism is formed between the protective pull bar and the tube body, and the tube body and the lower cover are kept relatively fixed by the positioning mechanism. .

Further, in the above technical solution, the positioning mechanism includes: a positioning groove arranged on the protective brace, a positioning block arranged on the pipe body, and the matching between the pipe body and the lower cover is made through the cooperation of the positioning groove and the positioning block. remain relatively fixed.

Further, in the above technical solution, a cutting groove is formed at the connection between the protective brace and the lower cover, and the protective brace has an end portion extending to the outside, and the end portion is pulled by an external force to make the protective brace Separate from the lower cover along the cutting groove.

Further, in the above technical solution, an arrow is formed on the lower cover, and a positioning slot corresponding to the arrow is formed on the outer wall of the tube body. When the lower cover is rotated at a certain angle, the arrow falls into the positioning slot. , so that the lower cover and the tube body remain relatively fixed.

Further, in the above technical solution, a partition frame is formed in the exchange chamber, and the sealing member has a sealing plate corresponding to the partition frame. When the sealing plate and the partition frame are at the same angle, the partition frame and the partition frame are at the same angle. The sealing plate seals and isolates the exchange cavity into a left space and a right space, wherein the left space is communicated with the main cavity, and the right space is communicated with the detection cavity.

Further, in the above technical solution, a partition is arranged between the main cavity and the exchange space, and a plurality of through holes are opened on the partition.

Further, in the above technical solution, the detection cavity has a label space arranged along the inner wall of the tube, and a test strip is arranged in the label space.

The invention also provides a method for using the composite tube for virus sampling, preservation and detection, which adopts the following technical scheme. The method of using the composite tube includes the following steps: in the first step, a virus preservation liquid is pre-injected into the main cavity, the collected test sample is added to the tube body of the composite tube through the upper end opening, and the upper end opening of the tube body is sealed through the upper cover. , at this time, the detection sample is mixed with the virus preservation liquid in the main cavity, and at the same time, the sealing member seals and separates the exchange cavity, so that the main cavity and the detection cavity cannot be communicated; the second step is to destroy the positioning mechanism on the lower cover, The lower cover can be rotated relative to the tube body, so that the sealing member is rotated in the exchange chamber by rotating the lower cover. During the rotation process, the sealing member no longer seals and separates the exchange chamber, and the main chamber and the detection chamber pass through the exchange chamber To achieve communication, let the sample enter the detection chamber, and after rotating a certain angle, the sealing element seals and separates the exchange chamber again, so that the main chamber and the detection chamber are sealed and isolated; the third step is to detect the mixed liquid of the sample and the virus preservation solution Enter the detection cavity, and a test strip is set in the detection cavity. After the mixed liquid soaks the test strip, a corresponding reaction occurs, causing the test strip to change accordingly. The user can observe the test strip through the tube body. changes to determine the detection results.

Compared with the prior art, the present invention has the following advantages: the present invention is simple and practical in structure and simple in operation, and can reduce the operation links of detection and save the detection cost. The invention can not only conduct on-site sampling and on-site rapid detection, but also can separate sampling and centralized detection. Because the samples are tested under sealed conditions, there is no risk of secondary transmission. It is suitable for large-scale rapid screening of infectious diseases, and can be applied to customs, airports, and schools. A crowded place such as a trading market. At the same time, the samples with positive test results of this product can be re-examined. At the same time, since the main cavity and the detection cavity are relatively sealed, there will be no cross-contamination.

Description of drawings:

Fig. 1 is the perspective view of the present invention;

Fig. 2 is the transverse sectional view of the present invention;

Fig. 3 is the longitudinal sectional view of the present invention;

Figure 4 is an exploded perspective view of the present invention;

Fig. 5 is the perspective view of the opening of the lower end of the pipe body of the present invention;

6 is a perspective view of the lower cover of the tube body of the present invention.

Detailed ways:

The present invention will be further described below with reference to specific embodiments and accompanying drawings.

As shown in FIGS. 1 to 6 , the present invention discloses a composite tube for virus sampling, preservation and detection. The composite tube includes: a tube body 1 , an upper cover 2 and a lower cover respectively installed on the upper and lower ends of the tube body 1 . 3.

The tube body 1 is made of a transparent material, such as a glass tube or a plastic tube. The inner cavity of the tube body 1 is divided into a main cavity 11 , an exchange cavity 12 and a detection cavity 13 . The upper end of the main cavity 11 is in communication with the upper end opening of the tube body 1 , and the lower end of the main cavity 11 is in communication with the exchange cavity 12 . The exchange cavity 12 is located at the lower end opening of the tube body 1 , and the main cavity 11 and the detection cavity 13 can communicate with each other through the exchange cavity 12 .

Since the main cavity 11 needs to be pre-injected with virus preservation liquid, the space of the main cavity 11 is relatively large. With reference to FIG. 2 , the upper half of the inner cavity of the tube body 1 and the space on one side of the lower half are used as the main cavity 11 . As shown in FIG. 6 , a partition 110 is provided between the main cavity 11 and the exchange cavity 12 , and a plurality of through holes 111 are formed on the partition 110 , and the main cavity 11 and the exchange cavity are realized through the through holes 111 . 12 connections.

The exchange cavity 12 is located below the main cavity 11 and the test cavity 13 , and the exchange cavity 12 communicates with the main cavity 11 and the test cavity 13 at the same time. A partition frame 120 is formed in the exchange chamber 12 . The partition frame 120 is located in the middle of the exchange cavity 12, and the exchange cavity 12 can be divided into a left space 1201 and a right space 1202 along the plane where the partition frame 120 is located, wherein the left space 1201 communicates with the main cavity 11, and the right space 1202 communicates with the detection cavity 13 .

The upper cover 2 is screw-tightly connected at the upper end opening of the pipe body 1 ; the lower cover 3 is rotatably installed at the lower end opening of the pipe body 1 . The connection between the lower cover 3 and the tube body 1 is as follows:

An annular flange 14 is provided on the outer wall of the pipe body 1 . The inner wall of the lower cover 3 is formed with clamping blocks 32 corresponding to the annular flange 14 . In this embodiment, a plurality of clamping blocks 32 are evenly distributed on the inner wall of the lower cover 3 . The snap connection realizes the connection between the lower cover 3 and the tube body 1 .

If only relying on the snap connection between the clamping block 32 and the annular flange 14, the tube body 1 and the lower cover 3 can still be rotated relative to each other. In the invention, the positioning is performed by protecting the brace 4 .

In this embodiment, the protection pull bar 4 is integrally formed with the lower cover 3 , and the protection pull bar 4 is fixedly connected to the upper edge of the lower cover 3 . The protective brace 4 forms a closed or semi-closed ring, and a positioning mechanism is formed between the protective brace 4 and the tube body 1 , through the positioning mechanism, the tube body 1 and the lower cover 3 are kept relatively fixed and cannot be opposed to each other. turn.

1 and 4 , the positioning mechanism includes: a positioning groove 42 arranged on the protective brace 4 , a positioning block 15 arranged on the pipe body 1 , and a matching order between the positioning groove 42 and the positioning block 15 The tube body 1 and the lower cover 3 remain relatively fixed and cannot rotate relative to each other.

When the lower cover 3 needs to be rotated, the protective brace 4 needs to be broken, so that the relatively fixed positioning mechanism between the tube body 1 and the lower cover 3 is broken. This embodiment adopts the following structure. As shown in FIG. 3 , a cutting groove 40 is formed at the connection between the protective brace 4 and the lower cover 3 , and the protective brace 4 has an end portion 41 extending to the outside, and the end portion 41 is pulled by an external force, The protective brace 4 is separated from the lower cover 3 along the cutting groove 40 . The design of the cutting groove 40 is to facilitate the separation of the protective pull strip 4 from the lower cover 3. Of course, other similar designs can also be used. For example, the cutting groove 40 is designed to be connected at several points. Point connection, you can separate the protection strip 4 and the lower cover 3.

The lower cover 3 has a sealing member 31 extending into the exchange cavity 12 , and rotating the lower cover 3 drives the sealing member 31 to rotate in the exchange cavity 12 , thereby realizing the exchange cavity connecting the main cavity 11 and the detection cavity 13 . Sealing or communication of the body 12 . The sealing member 31 has a sealing plate 310 corresponding to the partition frame 120. When the sealing plate 310 and the partition frame 120 are at the same angle, the left side of the cavity 12 is exchanged through the cooperation between the partition frame 120 and the sealing plate 310. The space 1201 and the right space 1202 are relatively sealed and isolated, so that the main cavity 11 communicated with the left space 1201 and the detection cavity 13 communicated with the right space 1202 are relatively sealed and isolated.

As shown in FIG. 4 , a sealing support plate 311 is formed on the inner surface of the lower cover 3 , and a layer of silicone material is wrapped on the surface of the sealing support plate 311 of the lower cover 3 by injection molding to form the sealing plate 310 .

Referring to FIG. 2 and FIG. 5 , the detection cavity 13 has a label space 131 disposed along the inner wall of the pipe body 1 , and the label space 131 is also communicated with the exchange cavity 12 . The test strip 5 is arranged in the label space 131 . The test strip 5 is arranged in the label space 131, and can be positioned to prevent its position from moving at will.

In addition, in order to ensure the angle of rotating the lower cover 3, an arrow 34 is formed on the lower cover 3, and a positioning slot 16 corresponding to the arrow 34 is formed on the outer wall of the tube body 1. When the lower cover 3 is rotated by a certain angle, the arrow 34 It falls into the positioning slot 16 to keep the lower cover 3 and the tube body 1 relatively fixed. Specifically to this embodiment, the following methods are adopted:

When the protection strip 4 is in the original state without being damaged, the sealing member 31 seals and separates the exchange cavity 12, the main cavity 11 and the detection cavity 13 cannot communicate with each other, and the position and positioning of the arrow 34 in this state The position of the card slot 16 is exactly on a straight line.

When the protection strip 4 is destroyed, the positioning mechanism between the tube body 1 and the lower cover 3 is destroyed. At this time, after the lower cover 3 is rotated 180°, the arrow 34 just falls into the positioning slot 16, and the lower cover 3 passes through the arrow 34. Coordination with the positioning slot 16 realizes positioning with the pipe body 1 . At this time, after the sealing plate 310 rotates 180° at the same time, at the same angle with the partition frame 120 again, the left space 1201 and the right space 1202 of the exchange chamber 12 are relatively sealed and isolated by the cooperation between the partition frame 120 and the sealing plate 310 . In this way, the main cavity 11 in communication with the left space 1201 and the detection cavity 13 in communication with the right space 1202 achieve relative sealing isolation again.

4, the shown positioning slot 16 includes a first positioning block 161 and a second positioning block 162 formed on the surface of the pipe body 1, between the first positioning block 161 and the second positioning block 162 The spacing is used as the groove body that can accommodate the arrow 34. In order to facilitate the rotation, the first positioning block 161 is formed with a guide slope toward the rotation direction of the lower cover 3 , through which the arrow 34 can smoothly pass over the first positioning block 161 and fall into the first positioning block 161 and the groove formed before the second positioning block 162 .

The above-mentioned first positioning block 161 and second positioning block 162 can also be used as positioning mechanisms for keeping the tube body 1 and the lower cover 3 relatively fixed. As shown in FIG. 6 , positioning grooves 421 and 422 corresponding to the first positioning locking block 161 and the second positioning locking block 162 may be formed on the protection brace 4 .

The method of using the composite pipe includes the following steps:

In the first step, the main cavity 11 is pre-injected with virus preservation liquid, the collected test samples are added to the tube body 1 of the composite tube through the upper end opening, and the upper end opening of the tube body 1 is sealed through the upper cover 2. At this time, The detection sample is mixed with the virus preservation liquid in the main cavity 11 , and at the same time, the sealing member 31 seals and separates the exchange cavity 12 , so that the main cavity 11 and the detection cavity 13 cannot communicate with each other.

In the second step, the positioning mechanism on the lower cover 3 is destroyed, that is, the end portion 41 is pulled by an external force, so that the protection strip 4 is separated from the lower cover 3 along the cutting groove 40 . In this way, the lower cover 3 loses the positioning mechanism, and can relatively rotate relative to the tube body 1 . By rotating the lower cover 3, the seal 31 is driven to rotate in the exchange cavity 12. During the rotation, the seal 31 no longer seals and separates the exchange cavity 12. The main cavity 11 and the detection cavity 13 are communicated through the exchange cavity. The mixed liquid of the sample and the virus preservation solution can enter the detection chamber 13 through the exchange chamber. After rotating for a certain angle (for example, rotating 180° as described above), the sealing member 31 seals and separates the exchange chamber 12 again, so that the main chamber 11 and the detection chamber 13 are sealed and isolated.

In the third step, the mixed liquid of the detection sample and the virus preservation solution enters the detection cavity 13, and the lower cover 3 is kept for a certain period of time (for example, 15 minutes) in the downward direction, so that the mixed liquid of the detection sample and the virus preservation solution is mixed with the detection cavity. The test strip 5 in the body 13 is fully infiltrated, and the test strip 5 produces a corresponding reaction, so that the test strip 5 produces corresponding changes, and the user can observe the change of the test strip 5 through the tube body 1, thereby Confirm the test result.

When the above second step is performed, the lower cover 3 is rotated, and when the arrow 34 rotates the positioning slot 16, the arrow just points to the position of the label space 131 where the test strip 5 is arranged.

The invention has simple and practical structure and simple operation, can reduce the operation links of detection and save the detection cost. The invention can not only conduct on-site sampling and on-site rapid detection, but also can separate sampling and centralized detection. Because the samples are tested under sealed conditions, there is no risk of secondary transmission. It is suitable for large-scale rapid screening of infectious diseases, and can be applied to customs, airports, and schools. A crowded place such as a trading market. At the same time, the samples with positive test results of this product can be re-examined. At the same time, since the main cavity and the detection cavity are relatively sealed, there will be no cross-contamination.

Of course, the above descriptions are only specific embodiments of the present invention, and are not intended to limit the scope of implementation of the present invention. Any equivalent changes or modifications made in accordance with the structures, features and principles described in the scope of the patent application of the present invention shall be included in the within the scope of the patent application of the present invention.

Claims (10)

1. A virus sampling, storing and detecting composite tube comprises: body (1), install respectively at body (1) upper and lower both ends open-ended upper cover (2) and lower cover (3), its characterized in that:

the inner cavity of the tube body (1) is provided with a main cavity (11), an exchange cavity (12) and a detection cavity (13) which are communicated with an opening at the upper end of the tube body (1) in a separated manner, wherein the main cavity (11) is communicated with the detection cavity (13) through the exchange cavity (12);

the lower cover (3) is provided with a sealing piece (31) extending into the exchange cavity (12), and the lower cover (3) is rotated to drive the sealing piece (31) to rotate in the exchange cavity (12), so that the exchange cavity (12) communicated with the main cavity (11) and the detection cavity (13) is sealed or communicated.

2. The virus sampling, storing and detecting composite tube of claim 1, wherein: the inner wall shaping of lower cover (3) have fixture block (32), body (1) outer wall on be provided with annular flange (14) that correspond with fixture block (32), be connected the realization lower cover (3) and body (1) through the buckle of fixture block (32) and annular flange (14).

3. The virus sampling, storing and detecting composite tube of claim 1, wherein: the protective device is characterized in that a protective brace (4) is fixedly connected to the lower cover (3), a positioning mechanism is formed between the protective brace (4) and the pipe body (1), and the pipe body (1) and the lower cover (3) are kept relatively fixed through the positioning mechanism.

4. The virus sampling, storing and detecting composite tube of claim 3, wherein: the positioning mechanism comprises: the positioning groove (42) is arranged on the protection brace (4), the positioning block (15) is arranged on the pipe body (1), and the pipe body (1) and the lower cover (3) are kept relatively fixed through the matching of the positioning groove (42) and the positioning block (15).

5. The virus sampling, storing and detecting composite tube of claim 3, wherein: the connection part of the protection brace (4) and the lower cover (3) is provided with a cutting groove (40), the protection brace (4) is provided with an end part (41) extending to the outside, and the end part (41) is pulled by external force to separate the protection brace (4) from the lower cover (3) along the cutting groove (40).

6. The virus sampling, storing and detecting composite tube of claim 1, wherein: an arrow (34) is formed on the lower cover (3), a positioning clamping groove (16) corresponding to the arrow (34) is formed on the outer wall of the tube body (1), and after the lower cover (3) is rotated for a certain angle, the arrow (34) falls into the positioning clamping groove (16), so that the lower cover (3) and the tube body (1) are kept relatively fixed.

7. The virus sampling, storing and detecting composite tube according to any one of claims 1 to 6, wherein: a separation frame (120) is formed in the exchange cavity (12), the sealing element (31) is provided with a sealing plate (310) corresponding to the separation frame (120), when the sealing plate (310) and the separation frame (120) are in the same angle state, the exchange cavity (12) is sealed and separated into a left space (1201) and a right space (1202) by the separation frame (120) and the sealing plate (310), wherein the left space (1201) is communicated with the main cavity (11), and the right space (1202) is communicated with the detection cavity (13).

8. The virus sampling, storing and detecting composite tube of claim 7, wherein: a partition plate (110) is arranged between the main cavity (11) and the exchange space (12), and a plurality of through holes (111) are formed in the partition plate (110).

9. The virus sampling, storing and detecting composite tube of claim 7, wherein: the detection cavity (13) is internally provided with a label space (131) arranged along the inner wall of the tube body (1), and the label space (131) is internally provided with a test strip (5).

10. The virus sampling, storing and detecting composite tube of claim 1, wherein: the use method of the composite pipe comprises the following steps:

firstly, virus preservation liquid is injected into a main cavity (11) in advance, a collected detection sample is added into a tube body (1) of the composite tube through an upper end opening, the upper end opening of the tube body (1) is sealed through an upper cover (2), at the moment, the detection sample is mixed with the virus preservation liquid in the main cavity (11), and meanwhile, a sealing element (31) seals and separates an exchange cavity (12) to ensure that the main cavity (11) cannot be communicated with a detection cavity (13);

secondly, destroying a positioning mechanism on the lower cover (3), enabling the lower cover (3) to rotate relatively to the pipe body (1), so that the lower cover (3) is rotated to drive the sealing element (31) to rotate in the exchange cavity (12), the sealing element (31) does not seal and separate the exchange cavity (12) in the rotating process, the main cavity (11) is communicated with the detection cavity (13) through the exchange cavity, a sample enters the detection cavity, and after the lower cover is rotated for a certain angle, the sealing element (31) seals and separates the exchange cavity (12) again, so that the main cavity (11) and the detection cavity (13) form sealed separation;

and thirdly, the mixed liquid of the detection sample and the virus preservation solution enters the detection cavity (13), a test strip (5) is arranged in the detection cavity (13), the test strip (5) is soaked by the mixed liquid to generate corresponding reaction, the test strip (5) generates corresponding change, and a user can observe the change of the test strip (5) through the tube body (1), so that the detection result is determined.

Images