CN111821059B - Mouse experimental device - Google Patents

Abstract

The invention relates to a mouse experiment device, which comprises a front baffle, a rear baffle, a left side plate, a right side plate, a top plate, a bottom plate and a blocking baffle, and the rear baffle, the top plate, the bottom plate and the blocking baffle enclose The formed area is the anesthesia area, the area enclosed by the front baffle, the top plate, the bottom plate, the left plate, the right plate and the blocking baffle is the active area. The baffle is located between the anesthesia area and the active area; an anesthetic is arranged in the anesthesia area, and the blocking baffle is a porous structure, so that after the anesthetic is volatilized, it can enter the active area through the blocking baffle to anesthetize the mice. The structure of the invention can quickly anesthetize mice, and avoid experimental mistakes or accidents caused by the fear of mice.

Description

A mouse experiment device

technical field

The invention relates to a medical experiment device, in particular to a mouse experiment device.

Background technique

The mouse experimental device is a conventional device for medical experiments. The experimental device includes a cavity for accommodating mice, and the cavity height is moderate. In the sleeping state, the experimenter can operate the mouse outside the experimental device, but the existing mouse experimental device is put into the mouse and then put in the anesthetic drug, the mouse enters the anesthesia state slowly, and the mouse enters the anesthesia after the mouse enters the anesthesia. Before the state, the mice will instinctively "resist", which affects the stability of the experimental device. In addition, some experimenters are afraid of mice, which in turn increases the possibility of experimental errors and improper handling leading to experimental accidents.

Therefore, it is necessary to provide a simple, safe and reliable mouse experimental device.

SUMMARY OF THE INVENTION

In order to solve the problem of the slow anesthesia of mice in the existing mouse experimental device, the experimenter cannot experiment out of fear. The invention provides a mouse experiment device with simple structure, safety and effectiveness.

A first aspect of the present invention provides a mouse experiment device, which includes a front baffle, a rear baffle, a left side plate, a right side plate, a top plate, a bottom plate and a blocking baffle, the rear baffle, the top plate, and the bottom plate The area enclosed by the blocking baffle is an anesthesia area, the area enclosed by the front baffle, the top plate, the bottom plate, the left side plate, the right side plate and the blocking baffle is an active area, and the anesthesia area and the active area are along the length of the experimental device The direction is set forward and backward, and the blocking baffle is located between the anesthesia area and the active area; an anesthetic is arranged in the anesthesia area, and the blocking baffle is a porous structure, so that after the anesthetic is volatilized, it can enter the active area through the blocking baffle to anesthetize the mice.

The active area is provided with a crossbar along the width direction of the experimental device, the length of the active area is a, the distance from the blocking baffle to the crossbar is b, and the average length of the existing experimental mice is c, the mice are from The distance from the nose to the forelimb is d, and the position of the crossbar satisfies b/a=(0.8-1.2)d/c. After the mouse enters the active area, the forelimb will take the initiative to grab the crossbar due to instinct. When the setting position of the crossbar satisfies the above relationship, the nose of the mouse is in close contact with the blocking baffle, so that the mouse can enter the anesthesia state in the shortest time. Preferably, b/a=(0.9-1.1)d/c.

As a preferred solution, the blocking baffle can be moved back and forth along the length direction of the mouse experimental device, so as to adjust the length of the crossbar to the blocking baffle, so that the distance between the crossbar and the blocking baffle is just enough for the mice The distance from the nose to the forelimbs.

As a preferred solution, the rear baffle can be moved back and forth along the length direction of the mouse experimental device, so as to adjust the length from the crossbar to the rear baffle, so that the distance from the crossbar to the blocking baffle is just enough for the mice the distance from the forelegs to the tail;

More preferably, the moving structure of the tailgate is provided with a locking structure. When the tailgate moves to a set position, the locking structure can fix the tailgate and prevent it from continuing to move.

Preferably, the diameter of the horizontal bar is 2-5 cm, and the mouse forelimb can hold 2/3 of the horizontal bar.

The horizontal bar can be rotated around the central axis, and when the mouse forelimbs grab the horizontal bar, the mouse can grab the horizontal bar with the forelimbs and run on the horizontal bar.

Preferably, the aperture of the porous separator is 2-3 mm, and the setting area of the holes can be set on the whole board, or can be a part of the area facing the mouse's mouth and nose. More than 60% of the total area of the plate, preferably 70-80%, can satisfy the requirement that the anesthetic can be volatilized from the anesthesia area and enter the active area at the first time, so that the mice can inhale as much anesthetic as possible.

As an embodiment of the present invention, a fan is provided on the rear baffle, and the fan is used to blow air into the anesthesia area. Due to the negative pressure effect, the anesthetic agent in the anesthesia area is more easily volatilized.

As another embodiment of the present invention, the anesthesia area is provided with an upper plate and a lower plate, and a closed structure is formed between the upper plate and the lower plate and the left and right side plates, the gaseous anesthetic enters the anesthesia area through the air inlet device, and then passes through the porous partition The holes in the plate allow the gaseous anesthetic to enter the active area.

The above two methods of anesthetics are not easy to control the dosage, and because the experimenter is facing the experimental device, after the volatile anesthetic is absorbed by the mice, the remaining anesthetic will enter the air and even be inhaled by the experimenter, which not only leads to waste of resources and pollution environment, and even endanger human health, resulting in experimental accidents.

As another embodiment of the present invention, a common baffle is used instead of the porous baffle, the baffle is provided with an air outlet, the rear baffle is provided with an air inlet, and a venturi is passed between the air inlet and the air outlet. The narrow-diameter section of the venturi tube is provided with a through hole, which is connected to the anesthesia area. The liquid volatile anesthetic is placed at the bottom of the anesthesia area, and volatilization forms steam to fill the entire anesthesia area. Negative pressure is generated at the through hole connecting the venturi tube and the anesthesia area, and the anesthetic vapor generated in the anesthesia area is inhaled into the venturi tube by negative pressure, and enters the active area with air or other corresponding carrier gas. In this way, the escape of the anesthetic vapor is the most moderate, and due to the effect of the carrier gas, the proportion of the anesthetic vapor is small, which will not cause pollution to the environment.

Preferably, the air outlet is facing the nasal cavity of the mouse, and the air outlet speed of the air outlet is less than 3m/s. When the wind speed is less than 3m/s, the mouse will not feel uncomfortable, not easily irritable, and easier to be anesthetized.

Preferably, the air inlet, the air outlet and the through hole connecting the venturi tube to the anesthesia area are all provided with control valves, and the control valves are connected through a controller, and the controller can choose any known form, when the controller When all valves are controlled to be closed, the anesthesia area is in a completely closed state, and the steam in the anesthesia area will not escape.

During the actual operation, the experimenter first puts the mice into the mouse experimental device. After the mice enter the active area, they will instinctively grab the crossbar with their forelimbs, and even step on the crossbar to run. When on the crossbar, the anesthetic blown from the anesthesia area can be better absorbed by the mice, especially during the running process of the mice, with the increase of the breathing volume, the amount of anesthetic inhaled by the mice per unit time further increases, The effect of anesthesia was further improved.

Preferably, the bottom plate and the left side plate, the right side plate, the front baffle and the rear baffle are detachable structures, so that after anesthesia, the mice lie on the bottom plate, and the bottom plate can be taken out from the upper space, which is convenient operate.

Further preferably, the inside of the bottom plate is a hollow structure, the hollow structure is provided with a counterweight block, and after the bottom plate is provided with a breeding block, even if the mice run and struggle in the experimental device, they can maintain good stability without Accidents such as overturning will occur, affecting the safety of the experiment.

The present invention has no special requirements for the materials of various substances, and can use any metal materials, such as iron, stainless steel, or plastic materials. Using plastic materials makes the structure more compact, the disassembly is convenient, and the overall experimental device is more portable. However, cleaning is inconvenient, and the metal material is heavy, especially for girls, it is inconvenient to disassemble, but due to the high surface energy of the metal, it is easy to clean.

There are no special requirements for the anesthetic agent of the present invention, and any known volatile drug that has an anesthetic effect on mice can be used in the present invention, such as enflurane. The anesthetic should be liquid or solid under normal temperature and pressure, which makes the feeding of the anesthetic more convenient.

As the second invention of the present invention, an experimental bench is provided, which includes the mouse experimental device, a lifting platform, an air inlet device, an air outlet device and a table as described above. The air outlet is connected to the air outlet, and the air outlet device is connected to the air outlet of the mouse experimental device through a conveying pipe (not shown). By setting up a lifting device on the experimental table, the experimenter can adjust the height of the mouse experimental device according to their height and operation conditions. Flexible adjustment to meet the needs of experimental operation.

Description of drawings

Figure 1 - a structural diagram of an embodiment of the present application;

Figure 2-bottom view of the base plate of the present application;

Figure 3 - a structural diagram of another embodiment of the present application;

Figure 4 - a schematic diagram of the Venturi tube of the present application;

Figure 5 - a laboratory table equipped with the mouse experimental device of the present application;

In the figure, 1-left side plate; 2-right side plate; 3-front baffle; 4-rear baffle; 5-top plate; 6-bottom plate; 7-cross bar; 8-fan; 9-blocking baffle; 10-counterweight; 11-through hole; 12-air inlet; 13-air outlet; 14-venturi; 100-mouse experimental device; 200-lifting device; 300-air inlet device; 400-air outlet device ; 500-desktop.

Detailed ways

Example 1

As shown in Figures 1-2, a mouse experimental device includes a front baffle 3, a rear baffle 4, a left side plate 1, a right side plate 2, a top plate 5, a bottom plate 6 and a blocking baffle 9. The area enclosed by the baffle 4, the top plate 5, the bottom plate 6 and the blocking baffle 9 is an anesthesia area. The formed area is the active area, the anesthesia area and the active area are arranged back and forth along the length direction of the experimental device, and the blocking baffle 9 is located between the anesthesia area and the active area; an anesthetic is provided in the anesthesia area, and the blocking baffle 9 is a porous structure, After the anesthetic is volatilized, it can enter the active area through the blocking baffle to anesthetize the mouse.

The active area is provided with a cross bar 7 along the width direction of the experimental device, and the blocking baffle 9 and the rear baffle 4 can be moved along the length direction of the experimental device to adjust the blocking baffle 9 and the rear baffle 4 and the cross bar. the distance.

The experimental device is made of stainless steel, the bottom of the bottom plate is a hollow structure, and seed mating blocks 10 are respectively provided on the left and right sides.

Example 2

As shown in Figures 3-4, a mouse experimental device includes a front baffle 3, a rear baffle 4, a left side plate 1, a right side plate 2, a top plate 5, a bottom plate 6 and a blocking baffle 9. The area enclosed by the baffle 4, the top plate 5, the bottom plate 6 and the blocking baffle 9 is an anesthesia area. The formed area is the active area, the anesthesia area and the active area are arranged back and forth along the length direction of the experimental device, and the blocking baffle 9 is located between the anesthesia area and the active area; an anesthetic is provided in the anesthesia area, and the blocking baffle is provided with an air outlet 13 , the rear baffle is provided with an air inlet 12, the air outlet 13 and the air inlet 12 are connected by a venturi tube, and a through hole 11 is provided on the venturi tube.

In actual operation, the carrier gas is introduced through the air inlet 12, and the carrier gas passes through the venturi 14 to form a negative pressure near the through hole 11, and the anesthetic vapor in the anesthesia area is inhaled by the negative pressure near the through hole, along with the carrier gas. are discharged from the air outlet 13 together. By adjusting the flow velocity of the air inlet, the wind speed of the air outlet is adjusted to 3m/s.

With this structure, the anesthetic does not pollute the environment and avoids harm to the experimental staff.

Example 3

As shown in FIG. 5 , the present embodiment relates to an experimental bench including the mouse experimental device of Embodiment 2, which includes the mouse experimental device 100 of Embodiment 2, a lifting platform 200 , an air intake device 300 , an air outlet device 400 and a table top 500 , the air inlet device 300 is connected to the air inlet of the mouse experimental device through a conveying pipeline, and the air outlet device is connected to the air outlet of the mouse experimental device through a conveying pipeline (not shown). The height of the mouse experimental apparatus was controlled by a control device (not shown) as required.

The above technical solutions only represent the preferred technical solutions of the technical solutions of the present invention, and some changes that those skilled in the art may make to some parts of them all reflect the principles of the present invention and fall within the protection scope of the present invention.

Claims (3)

1. A mouse experimental device is characterized by comprising a front baffle, a rear baffle, a left side plate, a right side plate, a top plate, a bottom plate and a blocking baffle, wherein the area surrounded by the rear baffle, the top plate, the bottom plate and the blocking baffle is an anesthesia area; an anesthetic is arranged in the anesthetic area; the bottom plate is internally provided with a hollow structure, and the hollow structure is provided with a balancing weight;

the experimental device comprises an active area, a barrier baffle, a transverse rod and a transverse rod, wherein the transverse rod is arranged at a position which satisfies the condition that b/a is (0.8-1.2) d/c, the distance from the nose to forelimbs of a mouse is d, and the average length of the existing experimental mouse is c;

the blocking baffle can move back and forth along the length direction of the mouse experimental device;

the blocking baffle is provided with an air outlet, the rear baffle is provided with an air inlet, the air inlet and the air outlet are connected through a Venturi tube, and a narrow-diameter section of the Venturi tube is provided with a through hole and is connected with an anesthesia area;

the liquid volatile anesthetic is placed at the bottom of the anesthetic region, volatilizes to form steam to fill the entire anesthetic region, when the air is introduced into the air inlet pipe, negative pressure is generated at a through hole of the venturi tube connected with the anesthetic region, anesthetic steam generated in the anesthetic region is sucked into the venturi tube by the negative pressure and enters the movable region along with air;

the air outlet speed of the air outlet is less than 3 m/s;

the cross bar can rotate around the central shaft, when the front limbs of the mouse grasp the cross bar, the front limbs can grasp the cross bar, and the running can be carried out on the cross bar.

2. The mouse experiment device as claimed in claim 1, wherein the rear baffle can move back and forth along the length direction of the mouse experiment device.

3. The device according to claim 1, wherein the diameter of the cross bar is 2-5 cm.

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