CN111812320B - Detection kit of full-automatic immunoassay device - Google Patents
Detection kit of full-automatic immunoassay device Download PDFInfo
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- CN111812320B CN111812320B CN202010739083.1A CN202010739083A CN111812320B CN 111812320 B CN111812320 B CN 111812320B CN 202010739083 A CN202010739083 A CN 202010739083A CN 111812320 B CN111812320 B CN 111812320B
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- 238000001514 detection method Methods 0.000 title claims abstract description 30
- 238000003018 immunoassay Methods 0.000 title claims abstract description 22
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 62
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 230000009471 action Effects 0.000 claims description 20
- 239000011324 bead Substances 0.000 claims description 20
- 238000012360 testing method Methods 0.000 claims description 13
- 230000003287 optical effect Effects 0.000 claims description 12
- 238000004891 communication Methods 0.000 claims description 11
- 238000009423 ventilation Methods 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 9
- 239000007789 gas Substances 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 239000011261 inert gas Substances 0.000 claims description 3
- 238000012423 maintenance Methods 0.000 claims description 3
- 229910052755 nonmetal Inorganic materials 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 abstract description 4
- 238000002347 injection Methods 0.000 abstract description 4
- 239000007924 injection Substances 0.000 abstract description 4
- 238000013461 design Methods 0.000 abstract description 2
- 238000000105 evaporative light scattering detection Methods 0.000 abstract description 2
- 239000007921 spray Substances 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 description 8
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004146 energy storage Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000010339 medical test Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000010248 power generation Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
- G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The utility model relates to a detection kit of a full-automatic immunoassay device. The kit has a simple structure, does not need excessive gear transmission, and adopts magnetic force control to quantitatively discharge liquid and spray; the positive pressure control is adopted, so that the liquid in the kit can overcome the surface tension and viscous force, and the quantitative injection precision is higher; the design is ingenious, positive pressure injection can be carried out at the same time by adopting only one group of rotating parts, and quantitative distribution and stirring of the reagent in the box body can be carried out; the full-automatic detection device is provided in a matched manner, has large capacity and high detection freedom, can simultaneously meet the requirements of mass detection and independent detection, and does not generate waste.
Description
Technical Field
The utility model relates to the field of medical analysis and detection, in particular to a detection kit of a full-automatic immunoassay device.
Background
An immunoassay instrument is a medical test instrument for performing an immunoassay on a human body by detecting serum of a patient. Quantitative patient serum and horseradish peroxidase (HRP) are added into a white opaque micro-pore plate with a solid phase coated with antibodies, and molecules to be detected in the serum are specifically combined with a conjugate of horseradish peroxidase and the antibodies on a solid phase carrier.
Application number 200910066010.4 discloses a detection kit for a full-automatic immunoassay analyzer: the reaction cup holder is arranged at the upper part of the base, the reaction cup holder and the base can be relatively fixed, and the centers on the base and the reaction cup holder are correspondingly provided with positioning devices corresponding to positioning shafts on the full-automatic immunoassay analyzer; holes which are arranged for accommodating the reaction cups in a circle are formed in the reaction cup holder by taking the center as the circle center, and reaction cup caps are correspondingly arranged on the reaction cups; the base is provided with a reagent bottle base for placing a reagent bottle, and the position of the reaction cup holder corresponding to the reagent bottle base on the base is provided with an opening for accommodating the reagent bottle base.
The application number 201921639877.X discloses a kit of a full-automatic immunoassay analyzer, which comprises a main reagent bottle, a magnetic bead reagent bottle and a magnetic bead reagent bottle rack, wherein a magnetic bead reagent bottle positioning ring is arranged on the outer wall of the magnetic bead reagent bottle, a magnetic bead reagent bottle hook is arranged on the side wall of the magnetic bead reagent bottle rack, and a main reagent bottle hook matched with the magnetic bead reagent bottle hook is arranged on the side part of the main reagent bottle. The split type detachable magnetic bead reagent bottle and the main reagent bottle are arranged between the magnetic bead reagent bottle and the main reagent bottle, and a gear at the bottom of the magnetic bead reagent bottle is meshed with a transmission gear at the center of the reagent disk to drive the magnetic bead reagent bottle to rotate, so that the effect of uniformly mixing reagents in the magnetic bead reagent bottle is realized. The magnetic beads are used for stirring the reagents, but the magnetic beads also use transmission mechanisms such as gears and the like, and have complex structure and poor stability.
In summary, the existing immunoassay kit is generally complicated in structure and poor in stability, while the kit with a simple structure is generally too few in functions and cannot meet the requirement of full-automatic detection.
Disclosure of Invention
In order to solve the above problems, the detection kit of the full-automatic immunoassay device is provided and comprises a box body, a cap and a back cover.
The box body is barrel-shaped, the upper part and the lower part of the box body are both open, an air bag capable of storing gas is arranged in the cover cap, and the cover cap can rotate around the axis of the box body under the action of an external magnetic field; the bottom cover is made of three layers of materials, namely an upper bottom, a middle bottom and a lower bottom, wherein the upper bottom is provided with a liquid inlet for the reagent to enter, the middle bottom is provided with a circulating hole for the reagent to flow through, and the lower bottom is provided with a discharge hole for the reagent to discharge;
the midsole can rotate around the axis of the kit body under the action of an external magnetic field, and the upper sole is provided with a vent pipe communicated with the cap; the rotation of the midsole cooperates with the rotation of the cap to achieve dosing of the reagent.
The box body is made of transparent nonmetal, the cover cap is made of rubber material, and a flange for installing the cover cap is arranged at the top of the box body, so that the cover cap can rotate around the axis of the box body under the condition of sealing an opening on the box body;
the central axis of the cap is provided with a hole for the needle to pass through, so that the syringe can be inserted during filling practice or the air supply needle can be inserted into the positive pressure in the maintenance kit during use; the bottom edge of the cap is provided with an air vent which is connected to the air bag in the cap; a first magnetic block is arranged in the cap in the direction opposite to the air vent;
the three layers of materials of the back cover can seal the bottom surface of the box body, and the central axis of the middle bottom of the upper bottom is provided with a rotating shaft, so that the middle bottom can rotate around the axis of the box body, and the upper bottom and the lower bottom are kept motionless;
a liquid inlet hole for allowing the reagent in the box body to flow into the midsole is formed in one side of the top surface of the upper sole, and the liquid inlet hole penetrates through the upper sole; the other side opposite to the liquid inlet hole is provided with a vent pipe connected to the cap, and the vent pipe is fixed on the inner surface of the box body and can be in sealing connection with the vent hole on the cap; the outlet at the other end of the vent pipe is positioned at the bottom surface of the upper bottom;
the middle sole is provided with a flow hole at a position corresponding to the liquid inlet hole, and a second magnetic block is arranged at one side of the middle sole opposite to the flow hole;
a plurality of discharge holes are arranged at the positions of the lower bottom corresponding to the circulation holes;
the cap and the lower bottom can synchronously rotate under the action of an external magnetic field, so that when the middle bottom rotates to the state that the flow hole is communicated with the liquid inlet, the reagent in the box body enters the communication hole under the action of positive pressure; meanwhile, the air bag of the cap and the air vent hole rotate to the other side of the vent pipe, and the air vent hole inflates the air bag under the action of positive pressure in the box body;
when the midsole rotates to the communication of the flow hole and the discharge hole, the vent hole is just communicated with the vent pipe, so that the pressure in the air bag is transmitted to the lower surface of the midsole through the vent pipe and just enters the communication hole of the midsole; the reagent in the communication hole is sprayed outside the box body through the discharge hole under the action of air pressure.
The full-automatic immunoassay device is provided with a rotary drum sleeved outside the box body, and a third magnetic block and a fourth magnetic block are arranged in the rotary drum; the position of the third magnetic block corresponds to the first magnetic block, and the position of the fourth magnetic block corresponds to the second magnetic block, so that when the rotary drum rotates, the third magnetic block and the fourth magnetic block drive the first magnetic block and the second magnetic block to synchronously rotate.
A plurality of magnetic beads are arranged in the box body, and the magnetic beads can stir the reagent in the box body under the action of an external magnetic field.
A fully automatic immunoassay device matched with the detection kit for use;
the rotary electric energy storage device comprises an outer cover, a main body and a chassis, wherein the main body is cylindrical, a driving shaft is arranged on the axis and connected to the chassis, a motor for driving the driving shaft to rotate is arranged in the chassis, and the main body and the chassis can mutually rotate;
a circle of rotary drums are arranged on the inner wall of the main body, and reagent boxes can be placed in the rotary drums; and one side of each rotary drum is provided with an independent driving roller which can drive the rotary drums to rotate;
the outer cover can be connected to the main body, and an air needle is arranged at a position of the outer cover corresponding to the rotary drum; the outer cover is internally provided with a pressure container, inert gas is stored in the pressure container, and the pressure container is connected to the gas needle; when the outer cover is covered on the main body, the air needle is inserted into the cover cap of the kit to provide positive pressure in the kit;
the chassis is provided with a plurality of test paper or chip mounting stations, and the position of each mounting station corresponds to the rotating bottom surface of the main body; when the test paper or the chip is rotated, the driving roller drives the rotary drum to rotate; the dosing of the reagent is completed once every rotation of the rotary drum, and the reagent is uniformly sprayed onto the test paper or the chip; the kit can store a detection reagent or a sample required for detection.
The device is also provided with a control chip for overall control and an optical detector, wherein the control chip is used for controlling the whole detection process, the optical detector is used for carrying out optical identification on test paper or the chip, converting optical signals into electric signals and sending the electric signals to the control chip, and the control chip converts the electric signals into detected data.
The beneficial effects of the utility model are as follows: the utility model provides a kit for full-automatic immunodetection, which has a simple structure, does not need excessive gear transmission, and adopts magnetic force control to quantitatively discharge liquid and spray; the positive pressure control is adopted, so that the liquid in the kit can overcome the surface tension and viscous force, and the quantitative injection precision is higher; the design is ingenious, positive pressure injection can be carried out at the same time by adopting only one group of rotating parts, and quantitative distribution and stirring of the reagent in the box body can be carried out;
the full-automatic detection device is provided in a matched manner, has large capacity and high detection freedom, can simultaneously meet the requirements of mass detection and independent detection, and does not generate waste.
Drawings
The accompanying drawings, which are included to provide a further understanding of the disclosed subject matter, are incorporated in and constitute a part of this specification. The drawings also set forth implementations of the disclosed subject matter and, together with the detailed description, serve to explain the principles of the implementations of the disclosed subject matter. No attempt is made to show structural details of the disclosed subject matter in more detail than is necessary for a fundamental understanding of the disclosed subject matter and its various ways of practice.
FIG. 1 is a schematic diagram of a kit of the present utility model;
FIG. 2 is a schematic view of the upper cover and main body of the detecting device of the present utility model;
fig. 3 is a schematic diagram of the detection device of the present utility model.
Detailed Description
The advantages, features and manner of attaining the stated objects of the utility model will become apparent from the description to follow, and from the drawings.
Example 1:
a detection kit of a full-automatic immunoassay device comprises a box body 1, a cover cap 2 and a back cover.
The box body 1 is barrel-shaped, openings are formed in the upper and lower sides, an air bag 21 capable of storing air is arranged in the cover cap 2, and the cover cap 2 can rotate around the axis of the box body 1 under the action of an external magnetic field; the back cover is made of three layers of materials, namely an upper bottom 31, a middle bottom 32 and a lower bottom 33, wherein the upper bottom 31 is provided with a liquid inlet 34 for the reagent to enter, the middle and lower parts are provided with a circulating hole 35 for the reagent to flow through, and the lower bottom 33 is provided with a discharge hole 36 for the reagent to discharge;
the midsole 32 can rotate around the axis of the kit body 1 under the action of an external magnetic field, and the upper midsole 31 is provided with a vent pipe 37 communicated with the cap 2; the rotation of the midsole 32 cooperates with the rotation of the cap 2 to effect the dosing of the reagent.
The box body 1 is made of transparent nonmetal, the cover cap 2 is made of rubber material, and a flange for installing the cover cap 2 is arranged at the top of the box body 1, so that the cover cap 2 can rotate around the axis of the box body 1 under the condition of sealing an opening on the box body 1;
the central axis of the cap 2 is provided with a hole 22 for the needle to pass through, so that a syringe can be inserted during filling practice or an air needle 61 can be inserted into the positive pressure in the maintenance kit during use; the bottom edge of the cap 2 is provided with an air vent hole 23, and the air vent hole 23 is connected into the air bag 21 in the cap 2; a first magnet 41 is provided inside the cap 2 in a direction opposite to the ventilation hole 23;
the three layers of materials of the back cover can seal the bottom surface of the box body 1, the central axis of the lower bottom 33 of the middle bottom 32 of the upper bottom 31 is provided with a rotating shaft, so that the middle bottom 32 can rotate around the axis of the box body 1, and the upper bottom 31 and the lower bottom 33 are kept motionless;
a liquid inlet hole 34 for allowing the reagent in the box body 1 to flow into the midsole 32 is formed in one side of the top surface of the upper sole 31, and the liquid inlet hole 34 penetrates through the upper sole 31; the other side opposite to the liquid inlet 34 is provided with a vent pipe 37 connected to the cap 2, and the vent pipe 37 is fixed on the inner surface of the box body 1 and can be in sealing connection with the vent hole 23 on the cap 2; the outlet of the other end of the vent pipe 37 is positioned on the bottom surface of the upper bottom 31;
the midsole 32 is provided with a circulation hole 35 at a position corresponding to the liquid inlet hole 34, and a second magnetic block 42 is arranged at one side of the midsole 32 opposite to the circulation hole 35;
a plurality of discharge holes 36 are provided at positions of the lower bottom 33 corresponding to the flow holes 35;
the cap 2 and the lower bottom 33 can synchronously rotate under the action of an external magnetic field, so that when the middle bottom 32 rotates to the state that the flow hole 35 is communicated with the liquid inlet 34, the reagent in the box body 1 enters the communication hole under the action of positive pressure; simultaneously, the air bag 21 and the air vent hole 23 of the cover cap 2 rotate to the other side of the vent pipe 37, and the air vent hole 23 inflates the air bag 21 under the action of positive pressure in the box body 1;
when the midsole 32 rotates to the communication between the flow hole 35 and the discharge hole 36, the ventilation hole 23 is just communicated with the ventilation pipe 37, so that the pressure in the air bag 21 is transmitted to the lower surface of the midsole 31 through the ventilation pipe 37 and just enters the communication hole of the midsole 32; the reagent in the communication hole is ejected to the outside of the cartridge 1 through the discharge hole 36 by the air pressure.
The full-automatic immunoassay device is provided with a rotary drum 5 sleeved outside the box body 1, and a third magnetic block 43 and a fourth magnetic block 44 are arranged in the rotary drum; the position of the third magnetic block 43 corresponds to the first magnetic block 41, and the position of the fourth magnetic block 44 corresponds to the second magnetic block 42, so that when the rotary drum rotates, the third magnetic block 43 and the fourth magnetic block 44 drive the first magnetic block 41 and the second magnetic block 42 to synchronously rotate.
A plurality of magnetic beads are arranged in the box body 1, and the magnetic beads can stir the reagent in the box body 1 under the action of an external magnetic field.
Example 2:
a fully automatic immunoassay device matched with the detection kit for use;
the novel electric power generation device comprises an outer cover 6, a main body 7 and a chassis 8, wherein the main body 7 is cylindrical, a driving shaft 71 is arranged on the axis, the driving shaft 71 is connected to the chassis 8, a motor for driving the driving shaft 71 to rotate is arranged in the chassis 8, and the main body 7 and the chassis 8 can mutually rotate;
a circle of rotary drum 5 is arranged on the inner wall of the main body 7, and a reagent box can be placed in the rotary drum; and a separate driving roller 72 is arranged on one side of each rotary drum, so that the rotary drums can be driven to rotate;
the outer cover 6 may be connected to the main body 7, and the outer cover 6 is provided with an air needle 61 at a position corresponding to the drum; a pressure vessel 62 is arranged in the outer cover 6, inert gas is stored in the pressure vessel 62, and the pressure vessel 62 is connected to the gas needle 61; when the outer cover 6 is covered on the main body 7, the air needle 61 is inserted into the cover cap 2 of the kit to provide positive pressure in the kit;
the chassis 8 is provided with a plurality of test paper or chip mounting stations, and the position of each mounting station corresponds to the rotating bottom surface of the main body 7; when the kit is rotated above the test paper or chip 81, the driving roller 72 drives the rotary drum to rotate; dosing of the reagent is completed once each rotation of the drum and the reagent is evenly sprayed onto the test paper or chip 81; the kit can store a detection reagent or a sample required for detection.
The device is also provided with a control chip for overall control and an optical detector, wherein the control chip is used for controlling the whole detection process, the optical detector is used for carrying out optical identification on test paper or chip 81, converting optical signals into electric signals and sending the electric signals to the control chip, and the control chip converts the electric signals into detected data. .
The above description is merely of the preferred embodiments of the present utility model, but the scope of the present utility model is not limited thereto, and any person skilled in the art can easily think about the changes or substitutions within the technical scope of the present utility model, and the changes or substitutions are intended to be covered by the scope of the present utility model. Therefore, the protection scope of the present utility model shall be subject to the protection scope of the claims.
Claims (5)
1. The utility model provides a full-automatic immunoassay device's detection kit, includes box body (1), lid (2) and back cover, its characterized in that:
the box body (1) is in a barrel shape, openings are formed in the upper and lower sides, an air bag (21) capable of storing air is arranged in the cover cap (2), and the cover cap (2) can rotate around the axis of the box body (1) under the action of an external magnetic field; the back cover is made of three layers of materials, namely an upper bottom (31), a middle bottom (32) and a lower bottom (33), wherein the upper bottom (31) is provided with a liquid inlet hole (34) for the reagent to enter, the middle bottom is provided with a circulating hole (35) for the reagent to flow through, and the lower bottom (33) is provided with a discharge hole (36) for the reagent to discharge;
the midsole (32) can rotate around the axis of the kit body (1) under the action of an external magnetic field, and the upper midsole (31) is provided with a vent pipe (37) communicated with the cap (2); the rotation of the midsole (32) is matched with the rotation of the cap (2) to realize the quantitative distribution of the reagent;
the bottom edge of the cap (2) is provided with an air vent (23), and the air vent (23) is connected into an air bag (21) in the cap (2); a first magnetic block (41) is arranged in the cap (2) in the direction opposite to the air vent (23); a second magnetic block (42) is arranged on one side of the midsole (32) opposite to the flow hole (35); the vent pipe (37) is fixed on the inner surface of the box body (1) and can be connected with the vent hole (23) on the cap (2) in a sealing way; the outlet at the other end of the vent pipe (37) is positioned at the bottom surface of the upper bottom (31);
the cap (2) and the midsole (32) can synchronously rotate under the action of an external magnetic field, so that when the midsole (32) rotates to the state that the circulation hole (35) is communicated with the liquid inlet hole (34), the reagent in the box body (1) enters the communication hole under the action of positive pressure; simultaneously, the air bag (21) and the ventilation hole (23) of the cap (2) rotate to the other side of the ventilation pipe (37), and the ventilation hole (23) inflates the air bag (21) under the action of positive pressure in the box body (1);
when the midsole (32) rotates to be communicated with the circulation hole (35) and the discharge hole (36), the ventilation hole (23) is just communicated with the ventilation pipe (37), so that the pressure in the air bag (21) is conveyed to the lower surface of the midsole (31) through the ventilation pipe (37) and just enters the communication hole of the midsole (32); the reagent in the communication hole is sprayed out of the box body (1) through the discharge hole (36) under the action of air pressure;
a plurality of magnetic beads are arranged in the box body (1), and the magnetic beads can stir the reagent in the box body (1) under the action of an external magnetic field.
2. The detection kit of a fully automatic immunoassay device of claim 1, wherein:
the box body (1) is made of transparent nonmetal, the cover cap (2) is made of rubber materials, and a flange for installing the cover cap (2) is arranged at the top of the box body (1), so that the cover cap (2) can rotate around the axis of the box body (1) under the condition that an upper opening of the box body (1) is sealed;
the central axis of the cap (2) is provided with a hole (22) for a needle to pass through, so that a syringe can be inserted during filling practice or a gas supply needle (61) can be inserted into a maintenance kit during use to maintain positive pressure;
the three layers of materials of the back cover can seal the bottom surface of the box body (1), and the central axis of the middle bottom (32) and the lower bottom (33) of the upper bottom (31) is provided with a rotating shaft, so that the middle bottom (32) can rotate around the axis of the box body (1), and meanwhile, the upper bottom (31) and the lower bottom (33) are kept motionless;
a liquid inlet hole (34) for allowing the reagent in the box body (1) to flow into the midsole (32) is formed in one side of the top surface of the upper sole (31), and the liquid inlet hole (34) penetrates through the upper sole (31); the other side opposite to the liquid inlet hole (34) is provided with a vent pipe (37) connected to the cap (2),
the midsole (32) is provided with a circulation hole (35) at a position corresponding to the liquid inlet hole (34),
a plurality of discharge holes (36) are provided in the lower bottom (33) at positions corresponding to the flow holes (35).
3. The detection kit of the fully automatic immunoassay device of claim 2, wherein:
the full-automatic immunoassay device is provided with a rotary drum (5) sleeved outside the box body (1), and a third magnetic block (43) and a fourth magnetic block (44) are arranged in the rotary drum; the position of the third magnetic block (43) corresponds to the first magnetic block (41), and the position of the fourth magnetic block (44) corresponds to the second magnetic block (42), so that when the rotary drum rotates, the third magnetic block (43) and the fourth magnetic block (44) drive the first magnetic block (41) and the second magnetic block (42) to synchronously rotate.
4. A fully automatic immunoassay device for use with the test kit of claim 3, wherein:
the novel rotary electric machine comprises an outer cover (6), a main body (7) and a chassis (8), wherein the main body (7) is cylindrical, a driving shaft (71) is arranged on the axis of the main body, the driving shaft (71) is connected to the chassis (8), a motor for driving the driving shaft (71) to rotate is arranged in the chassis (8), and the main body (7) and the chassis (8) can mutually rotate;
a circle of rotary drum (5) is arranged on the inner wall of the main body (7), and a reagent box can be placed in the rotary drum; and one side of each rotary drum is provided with an independent driving roller (72) which can drive the rotary drums to rotate;
the outer cover (6) can be connected to the main body (7), and an air needle (61) is arranged at the position of the outer cover (6) corresponding to the rotary drum; a pressure container (62) is arranged in the outer cover (6), inert gas is stored in the pressure container (62), and the pressure container (62) is connected to the gas needle (61); when the outer cover (6) is covered on the main body (7), the air needle (61) is inserted into the cover cap (2) of the kit to provide positive pressure in the kit;
a plurality of test paper or chip mounting stations are arranged on the chassis (8), and the position of each mounting station corresponds to the rotating bottom surface of the main body (7); when the kit is in operation, the main body (7) rotates around the axis under the driving of the motor according to the requirement, and when the required kit rotates to the position above the test paper or the chip (81), the driving roller (72) drives the rotary drum to rotate; dosing of the reagent is completed once every rotation of the drum, and the reagent is uniformly sprayed onto the test paper or chip (81); the kit can store a detection reagent or a sample required for detection.
5. The fully automatic immunoassay device of claim 4, wherein:
the device is also provided with a control chip for overall control and an optical detector, wherein the control chip is used for controlling the whole detection flow, the optical detector is used for carrying out optical identification on test paper or a chip (81), converting an optical signal into an electric signal and sending the electric signal to the control chip, and the control chip converts the electric signal into detected data.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010739083.1A CN111812320B (en) | 2020-07-28 | 2020-07-28 | Detection kit of full-automatic immunoassay device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010739083.1A CN111812320B (en) | 2020-07-28 | 2020-07-28 | Detection kit of full-automatic immunoassay device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111812320A CN111812320A (en) | 2020-10-23 |
| CN111812320B true CN111812320B (en) | 2023-04-25 |
Family
ID=72862968
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010739083.1A Active CN111812320B (en) | 2020-07-28 | 2020-07-28 | Detection kit of full-automatic immunoassay device |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002037078A2 (en) * | 2000-10-31 | 2002-05-10 | Dpc Cirrus, Inc. | Automated immunoassay analyzer and method of using the same |
| CN101672842A (en) * | 2009-09-01 | 2010-03-17 | 郑州博赛生物技术股份有限公司 | Detection kit for fully automatic immunity analyzer |
| CN201514364U (en) * | 2009-09-28 | 2010-06-23 | 深圳市凯特生物医疗电子科技有限公司 | Device for detecting carbon dioxide content in blood serum or blood plasma |
| CN102046770A (en) * | 2008-07-01 | 2011-05-04 | 日商乐华股份有限公司 | Thermostat |
| CN103597065A (en) * | 2011-06-14 | 2014-02-19 | 日商乐华股份有限公司 | Constant-temperature device provided with rotating specimen table |
| CN107656078A (en) * | 2017-11-13 | 2018-02-02 | 山东朱氏堂医疗器械有限公司 | A kind of kit for quantitatively detecting NGAL in urine or blood |
| WO2019145415A1 (en) * | 2018-01-24 | 2019-08-01 | Insulcloud, S.L. | Monitoring device with universal adapter to drug injection pens |
| CN110237871A (en) * | 2019-07-11 | 2019-09-17 | 武汉中浩生物技术有限公司 | A kind of stomach Function detection luminescence immunoassay kit |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10360526A1 (en) * | 2003-12-22 | 2005-07-14 | Roche Diagnostics Gmbh | Reagent cassette with reagent container for particle-containing reagent for its noninvasive homogenization |
| US9797916B2 (en) * | 2014-01-10 | 2017-10-24 | Idexx Laboratories, Inc. | Chemical analyzer |
-
2020
- 2020-07-28 CN CN202010739083.1A patent/CN111812320B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002037078A2 (en) * | 2000-10-31 | 2002-05-10 | Dpc Cirrus, Inc. | Automated immunoassay analyzer and method of using the same |
| CN102046770A (en) * | 2008-07-01 | 2011-05-04 | 日商乐华股份有限公司 | Thermostat |
| CN101672842A (en) * | 2009-09-01 | 2010-03-17 | 郑州博赛生物技术股份有限公司 | Detection kit for fully automatic immunity analyzer |
| CN201514364U (en) * | 2009-09-28 | 2010-06-23 | 深圳市凯特生物医疗电子科技有限公司 | Device for detecting carbon dioxide content in blood serum or blood plasma |
| CN103597065A (en) * | 2011-06-14 | 2014-02-19 | 日商乐华股份有限公司 | Constant-temperature device provided with rotating specimen table |
| CN107656078A (en) * | 2017-11-13 | 2018-02-02 | 山东朱氏堂医疗器械有限公司 | A kind of kit for quantitatively detecting NGAL in urine or blood |
| WO2019145415A1 (en) * | 2018-01-24 | 2019-08-01 | Insulcloud, S.L. | Monitoring device with universal adapter to drug injection pens |
| CN110237871A (en) * | 2019-07-11 | 2019-09-17 | 武汉中浩生物技术有限公司 | A kind of stomach Function detection luminescence immunoassay kit |
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| CN111812320A (en) | 2020-10-23 |
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