CN111751558B - Fully automatic fluorescent immunoassay analyzer and sample detection method - Google Patents
Fully automatic fluorescent immunoassay analyzer and sample detection method Download PDFInfo
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- CN111751558B CN111751558B CN201910736617.2A CN201910736617A CN111751558B CN 111751558 B CN111751558 B CN 111751558B CN 201910736617 A CN201910736617 A CN 201910736617A CN 111751558 B CN111751558 B CN 111751558B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1065—Multiple transfer devices
- G01N35/1067—Multiple transfer devices for transfer to or from containers having different spacing
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- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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Abstract
The invention discloses a full-automatic fluorescence immunoassay analyzer and a sample detection method, wherein the analyzer comprises a bracket, the bracket is arranged on a base, a sample injection and discharge device is arranged on the base and used for pushing a sample tube into the sample injection for detection and pushing the sample tube out of the sample, a sample tube mixing component is arranged on the side surface of the sample injection and discharge device and used for mixing samples in the sample tube, the sample tube is pushed into the sample injection by the sample injection and discharge device for detection and pushing out of the sample, the analyzer further comprises a sampling needle movement component and used for extracting the samples in the sample tube, a reagent bin mechanism is arranged in the bracket, a plurality of reagent cards are stacked in the reagent bin mechanism, the diluted samples are added into a sample adding port of the reagent cards by a sampling needle, and a light path detection component for detecting the reagent cards is also arranged in the bracket. The analyzer has reasonable and compact overall layout and complete functions, realizes full-automatic operation, has humanized design, and is efficient and quick.
Description
Technical Field
The invention belongs to the field of fluorescence immunoassay detection, and particularly relates to a full-automatic fluorescence immunoassay analyzer and a sample detection method.
Background
The fluorescent immunodetection technology is a detection technology commonly used in biomedical detection at present, and the technology is used for qualitatively and quantitatively detecting an analyte by utilizing the characteristic that the analyte generates fluorescence under the excitation of light with a specific wavelength. Because the fluorescence immunoassay technology has a series of advantages of high sensitivity, strong specificity, high detection speed, safety, stability and the like, the fluorescence immunoassay technology is widely applied to clinical detection, and has wide application prospects in the detection fields of endocrinopathy detection, infectious disease detection, gynecological disease detection, tumor marker detection, genetic disease detection, blood and cytology detection and the like.
The traditional fluorescence immunoassay analyzer is large in size and inconvenient to use in a handheld manner, so that the development of the fluorescence immunoassay analyzer in the field of emergency detection (such as emergency laboratories, ICU, pediatrics, 120 emergency ambulances, outdoor emergency rescue, individual rescue), inconvenient transportation and underdeveloped areas is limited. Even if some commercial products exist, the defects of single detection wave band, complex structure, poor reliability, low precision and the like exist.
The invention discloses an automatic dry fluorescence detector in China patent document CN106198480A, which comprises a sample inlet and outlet mechanism, a sampling mechanism, a feeding mechanism, a swinging mechanism and a reagent feeding mechanism, wherein the sample inlet and outlet mechanism comprises a sample transmission device, a test tube rack capable of accommodating a plurality of sample tubes and a test tube rack accommodating plate, the test tube rack is arranged on the test tube rack accommodating plate, the test tube rack can convey the sample tubes from a position to be detected to the sampling position under the driving of the sample transmission device, the sampling mechanism comprises a sampling device, a liquid transferring device and a buffer zone for storing a plurality of buffer liquid boxes, the sampling device is driven by the liquid transferring device to switch back and forth among the sampling position, the buffer liquid boxes and the sampling position, the turntable mechanism comprises a turntable and a turntable driving device for driving a loading disc to rotate, one end of the turntable is provided with a plurality of carrying clamp tongues for accommodating reagent cards, the turntable extends to the edge of the turntable, the turntable is driven by the turntable driving device to rotate to enable the carrying clamp tongues to reach different angles and be positioned at least two sampling positions, the detection position, the swinging mechanism comprises a feeding clamp device, the swinging device and at least two clamp boxes, each clamp box is arranged at the periphery of the position corresponding to the feeding clamp boxes, and each clamp box is arranged at the periphery of the position corresponding to the detection clamp boxes, and the feeding clamp boxes can be connected with the detection clamp boxes along the periphery, and the radial direction of the detection clamp feeding device, and the detection clamp boxes can be connected with the corresponding to the detection clamp boxes, and the detection clamp boxes can be arranged at the position and the position.
The automatic dry type fluorescence detector of the technical scheme has the advantages of poor effect, poor humanized design and deficient function in the actual use process, so that the existing fluorescence immunoassay device needs to be improved, and a novel full-automatic fluorescence immunoassay device and a sample detection method are developed.
Disclosure of Invention
The invention aims to solve the problem of providing a full-automatic fluorescence immunoassay analyzer.
In order to solve the technical problems, the technical scheme adopted by the invention is that the full-automatic fluorescence immunoassay analyzer comprises a bracket, wherein the bracket is arranged on a base, a sample feeding and discharging device is arranged on the base, and the sample feeding and discharging device is used for pushing a sample tube into a sample for detection and pushing the sample tube out for sample discharging;
The side of the sample injection and sample discharge device is provided with a sample tube mixing assembly which is used for mixing samples in the sample tube uniformly, wherein the sample tube is pushed into the sample injection by the sample injection and sample discharge device for detection and pushing out for sample discharge;
The sampling needle moving assembly is used for extracting samples in the sample tube, and is provided with a sampling needle;
a reagent bin mechanism is arranged in the bracket, a plurality of reagent cards are stacked in the reagent bin mechanism, and the sampling needle adds diluted samples to a sample adding port of the reagent cards;
And an optical path detection component for detecting the reagent card is also arranged in the bracket.
The full-automatic fluorescence immunoassay analyzer of the invention has the advantages that all parts are arranged in a concentrated way, so that the whole analyzer is reasonable and compact in layout, complete in functions, full-automatic operation is realized, a sample tube mixing assembly realizes a mixing function, a plurality of reagent cards are stacked in a reagent bin mechanism, a box is arranged once, repeated uninterrupted detection can be carried out, a light path detection assembly is arranged in a bracket and used for detecting fluorescent reaction signals of the reagent cards, and the full-automatic fluorescence immunoassay analyzer is comprehensive in function, humanized in design, efficient and quick.
The sample injection and sample discharge device comprises a sample injection pushing hand structure, a sample injection transverse pushing hand structure and a sample discharge pushing hand structure, wherein the sample injection pushing hand structure is used for pushing a sample tube placed on a sample injection platform into sample injection along the longitudinal direction, the sample injection transverse pushing hand structure is used for pushing the sample tube into sample injection along the transverse direction for detection, and the sample discharge pushing hand structure is used for pushing the detected sample tube to a sample discharge platform for sample discharge.
The sample injection pushing handle structure is provided with a sample injection pushing handle, the sample injection pushing handle is arranged on the guide rail I through a sliding block assembly I, the sliding block assembly I is fixed on the belt I, and the motor I drives the sliding block assembly I to longitudinally reciprocate along the guide rail I through a driving wheel I, a driven wheel I and the belt I so as to further longitudinally push a sample tube into sample injection.
The sample injection pushing hand structure is used for pushing a sample tube placed on the sample injection platform longitudinally and working together with the sample injection transverse pushing hand structure to further push the sample tube transversely into sample injection; by adopting the structure, the first slider component is fixed on the first belt, the first slider component is driven to longitudinally reciprocate along the first guide rail by the movement of the first belt, the structure is simple, the response is rapid, the first guide rail is used for guiding, the movement direction of the sample tube is stable, and the error rate is low.
The sample discharging pushing handle structure is provided with a sample discharging pushing handle, the sample discharging pushing handle is arranged on a guide rail III through a sliding block assembly III, the sliding block assembly III is fixed on a belt III, and a motor III drives the sliding block assembly III to longitudinally reciprocate along the guide rail III through a driving wheel III, a driven wheel III and the belt III, so that a detected sample tube is pushed out to a sample discharging platform for sample discharging.
The sample injection transverse pushing and pulling hand structure is provided with a plurality of transverse pulling hands, the transverse pulling hands are arranged on a guide rail II through a slide block assembly II, the slide block assembly II is fixed on a belt II, a motor II drives the slide block assembly II to transversely reciprocate along the guide rail II through a driving wheel II, a driven wheel II and the belt II, and then a sample tube is transversely pushed into the sample injection for detection, the sample tube is placed on a test tube rack with a plurality of test tube rack locating grooves, and the distance between two adjacent transverse pulling hands is not larger than the length of the test tube rack.
The sample feeding transverse pushing hand and the reagent bin hand are the same in structure, the diluting cup hand is provided with a hand head part, an elastic piece (generally a spring) is arranged below the hand head part, the hand tail part is movably connected with the sliding block assembly, the hand head part is connected with the hand tail part through an inclined part, the transverse hand motion is that the sliding block assembly is driven by starting a motor, then the transverse hand is driven to move, one sample tube position of a test tube rack is advanced once, then the transverse hand moves backwards, the hand head part is pressed backwards under the action of the bottom of the test tube rack, then one sample tube position is advanced under the action of the motor, the test tube rack is pushed to advance through repeated motion, and the distance between two adjacent transverse hand fingers is not larger than the length of the test tube rack, so that the transverse hand is pushed at the bottom of the test tube rack.
Preferably, the sample feeding and discharging device further comprises a transverse moving track, and the test tube rack performs sample feeding and discharging along the transverse moving track.
The transverse movement track is arranged, so that sample injection and sample discharge of the test tube rack are orderly and orderly, and the integral structure is optimized.
Preferably, the sample tube mixing assembly is provided with a swing arm for mixing samples, and the swing arm is connected with a rotating shaft of a swing motor to drive the sample tube to swing left and right so as to mix the samples in the sample tube.
Preferably, the swing motor is connected with a horizontal sliding block assembly, the horizontal sliding block assembly is driven by the horizontal motor to horizontally move along a horizontal guide rail, the horizontal motor is connected with an upper fixing block and a lower fixing block, the upper fixing block and the lower fixing block are connected with an upper sliding block assembly and a lower sliding block assembly, the upper sliding block assembly and the lower sliding block assembly are arranged on the upper guide rail and the lower guide rail, and the upper fixing block and the lower fixing block are driven by the upper motor and the lower motor to enable the upper fixing block and the lower fixing block to move up and down along the upper guide rail and the lower guide rail.
The horizontal and vertical direction are switched, so that the sample tube mixing assembly can accurately and rapidly grasp and lift the sample tube, and preparation is made for swing mixing.
The upper and lower motors and the horizontal motor are both screw rod motors, an up-and-down motion optocoupler is arranged on the side face of the upper and lower motors, correspondingly, an up-and-down motion sensing sheet is arranged on the upper and lower sliding block assembly, a horizontal motion optocoupler is arranged on the side face of the horizontal motor, correspondingly, a horizontal motion sensing sheet is arranged on the horizontal sliding block assembly.
The screw rod motor is controllable and accurate in moving position, the sensing piece is matched with the optical coupler to control the distance of the swing arm moving up and down and horizontally, the optical coupler is matched with the sensing piece, and when the sensing piece moves to the position of the optical coupler, the optical coupler senses signal change, and then the motor stops working.
Preferably, the swing arm is provided with a spring clip for clamping the sample tube for uniformly mixing the sample, and the spring clip is used for tensioning to a certain degree, so that the sample tube is not damaged, and the sample tube cannot fall off when being grabbed or uniformly mixed and swung.
The sampling needle moving assembly comprises a sampling needle up-down moving assembly and a sampling needle horizontal moving assembly, wherein the sampling needle up-down moving assembly is provided with a sampling needle up-down moving motor, the sampling needle is arranged on a sampling needle up-down moving sliding block assembly in the sampling needle moving assembly, the sampling needle up-down moving motor drives the sampling needle up-down moving sliding block assembly to reciprocate along a sampling needle up-down guide rail, the sampling needle horizontal moving assembly is provided with a sampling needle horizontal moving motor, the sampling needle horizontal moving motor is connected with the sampling needle horizontal moving sliding block assembly through a fixed vertical plate, and the sampling needle horizontal moving motor drives the sampling needle horizontal moving sliding block assembly to reciprocate on the sampling needle horizontal guide rail.
The sampling needle is switched between the horizontal direction and the vertical direction by the sampling needle up-and-down motion assembly and the sampling needle horizontal motion assembly, so that the sampling needle can accurately extend into the sample tube to sample, and the fixed vertical plate is used for installing all components in the sampling needle up-and-down motion assembly.
The sampling needle horizontal movement motor is a stepping motor, the sampling needle horizontal movement motor drives the sampling needle horizontal movement driving wheel to rotate, the sampling needle horizontal movement belt sleeved on the sampling needle horizontal movement driving wheel drives the sampling needle horizontal movement driven wheel, and the fixed vertical plate is connected with the sampling needle horizontal movement belt through the sampling needle horizontal movement sliding block assembly.
Preferably, the sampling needle moving assembly further comprises a guiding bending belt, one end of the guiding bending belt is fixed above the fixed vertical plate, the other end of the guiding bending belt is arranged on the sampling needle moving assembly supporting frame, and the guiding bending belt is longitudinally arranged along the horizontal guide rail of the sampling needle.
In addition, the inside of the guiding bending belt is provided with a circuit or a liquid pipeline, so that the circuit or the liquid pipeline is not exposed, and the guiding bending belt is neat and attractive.
Preferably, the upper ends of the upper and lower guide rails of the sampling needle are provided with limiting optocouplers for the up-down movement of the sampling needle on the fixed vertical plate; the sampling needle moving assembly is characterized in that a sampling needle horizontal movement limiting optocoupler is arranged on the supporting frame of the sampling needle moving assembly and located at the tail part of the end close to the sampling needle horizontal guide rail.
Preferably, the sampling needle is arranged on the up-and-down motion sliding block assembly of the sampling needle, and the fixed vertical plate is also provided with a dilution needle.
The movement direction of the dilution needle is controlled through the set of sampling needle movement assembly, the structure is simple, the cost is saved, the programmed control is easy, and the automation degree is high.
Preferably, the reagent bin mechanism comprises a reagent bin bracket arranged on the base, a plurality of mutually independent reagent bins are arranged on the reagent bin bracket, a plurality of reagent cards can be stacked in each reagent bin, a plurality of mutually independent reagent bin hand pulling devices are correspondingly arranged at the bottom of each reagent bin and in the reagent bin bracket, and the reagent bin hand pulling devices are used for pulling the reagent cards in the reagent bins to a reagent card detection track in the bracket.
Preferably, the reagent bin is provided with a reagent card detector on the inner side wall, the reagent card detector is used for detecting the number of reagent cards, a fixed block corresponding to each reagent bin is further arranged on the reagent bin support, and a magnet is arranged on the reagent bin or the fixed block.
The fixed block is used for fixing the reagent bin inserted into the reagent bin bracket, and the magnet is arranged on the reagent bin or the fixed block, so that the reagent bin is further ensured to be inserted in place and is accurate.
The reagent card detector is arranged on the side wall of the reagent bin and can detect the number of the reagent cards to remind the timely addition of the reagent cards, and the magnet is tightly combined with the fixed block when the reagent bin is pulled out to put the reagent cards and then pushed in. The magnet can be a magnet arranged at the bottom of the rear wall of the reagent bin and the fixed block or one magnet is arranged on the bottom of the rear wall of the reagent bin and the fixed block, the other magnet is metal, the reagent bin push-in-place sensor is arranged on the fixed block to ensure that the reagent bin is pushed in place, 20-30 reagent cards can be placed in one magazine, and the magazine can be placed according to detection items and configured according to required quantity.
Preferably, the reagent bin hand-pulling device is provided with a reagent bin hand-pulling machine, the reagent bin hand-pulling machine drives a hand-pulling sliding block assembly fixedly connected with the hand-pulling belt to move back and forth along a hand-pulling guide rail through a hand-pulling driving wheel, a hand-pulling driven wheel and a hand-pulling belt, and the hand-pulling sliding block assembly is provided with a reagent bin hand-pulling device.
Preferably, the reagent bin handle comprises a handle head, an elastic piece is arranged below the handle head, the reagent bin handle is further provided with a handle tail which is movably connected with the handle sliding block assembly, the handle head is connected with the handle tail through an inclined part, and an upper pressing plate is arranged on the reagent bin support.
After the reagent bin hand-pulling moves to the reagent card detection track, the reagent bin hand-pulling moves to the initial position backward, the hand-pulling head is pressed down under the action of the spring (elastic piece) and the upper pressing plate, and when the hand-pulling hand continues to pull the reagent card to the detection track, the hand-pulling head is sprung to push the reagent card to the reagent card detection track.
Preferably, the reagent bin is also provided with a reagent bin handle, and the fixed block is provided with a reagent bin in-place pushing sensor.
The reagent bin handle is convenient for pushing and pulling the reagent bin, the upper cover is opened after the reagent bin is pulled out, and the reagent card is put in, and the reagent bin push-in-place sensor detects whether the reagent bin is pushed in place so as to be matched with other components, in particular to the reagent bin hand pulling device, which needs to pull the reagent card in the reagent bin to the reagent card detection track in the bracket, so that the position requirement on the reagent bin is strict.
Preferably, the reagent bin bracket is also provided with a plurality of mutually independent reagent bin grooves, and the upper parts of the reagent bin grooves are fixedly connected through a fixing plate.
The reagent bin groove is used for inserting the reagent bin, and the reagent bin groove top is fixed through the fixed plate connection for the reagent bin does not rock in the lateral direction sways.
Preferably, the optical path detection assembly is positioned in the bracket and comprises an optical path detection assembly fixing block, the optical path detection assembly fixing block is connected with an optical path detection assembly sliding block assembly, the optical path detection assembly sliding block assembly is positioned on an optical path detection assembly translation guide rail, the optical path detection assembly further comprises an optical path detection assembly translation motor, the optical path detection assembly translation motor drives the optical path detection assembly fixing block to reciprocate along the optical path detection assembly translation guide rail, and the optical pickup head assembly is arranged on the optical path detection assembly fixing block.
The fixing block of the light path detection assembly moves reciprocally along the translation guide rail of the light path detection assembly, so that the light path detection assembly moves translationally in the bracket, and the reagent card is conveniently searched by the light extraction head assembly.
Preferably, the light path detection assembly fixing block is further provided with a lighting head moving motor, the lighting head assembly is connected with the lighting head sliding block assembly, and the lighting head moving motor drives the lighting head assembly to reciprocate along the direction perpendicular to the translation guide rail of the light path detection assembly.
By adopting the structure, the lighting head assembly can reciprocate and scan the searched reagent card, and can reciprocate and collect the optical signal at the detection window of the reagent card.
Preferably, the lighting head component comprises an upper shielding cover and a lower lighting head, and can be used for collecting optical signals by moving back and forth through a window arranged on the light path detection component fixing block and a reagent card detection window positioned below.
Preferably, the optical path detection component fixing block is connected with one end of the detection component guiding bending belt, and the detection component guiding bending belt is arranged along the direction of the optical path detection component translation guide rail and is positioned on the side surface of the optical path detection component translation guide rail.
In addition, the inside of the guiding bending belt is provided with a circuit or a liquid pipeline, so that the circuit or the liquid pipeline is not exposed, and the lighting head is tidy and attractive.
Preferably, the full-automatic fluorescence immunoassay analyzer further comprises an emergency sample placement position, wherein the emergency sample placement position is used for placing a sample tube and is provided with an emergency position and an emergency sample tube detection sensor, and the emergency sample placement position is positioned in the horizontal movement direction of the sampling needle.
The emergency sample tube detection sensor is used for detecting whether the emergency sample tube is placed in place or not and then continuing the detection process, and the emergency sample placement position is arranged on the horizontal movement direction of the sampling needle, so that the horizontally moved sampling needle can accurately extend into the emergency sample tube for sampling.
Preferably, the full-automatic fluorescence immunoassay analyzer is provided with a test tube rack detection sensor, a test tube rack positioner and a sample tube detection sensor at the rear side of the sample injection and discharge device, wherein the test tube rack detection sensor is arranged on a vertical plate of the bracket, and the test tube rack positioner and the sample tube detection sensor sense through corresponding windows of the vertical plate of the bracket.
The test tube rack detection sensor is used for detecting whether the test tube rack is pushed into a preset position or not, the test tube rack positioner is used for positioning, and the sample tube detection sensor is used for detecting whether the sample tube is positioned at the preset position or not.
The sample code scanning device is arranged above the sample tube detection sensor, a code scanning rotating mechanism is arranged close to the sample code scanning device and comprises a sample tube rotating motor, the sample tube rotating motor drives a rotating wheel to rotate, the code scanning rotating mechanism further comprises a first rotating wheel and a second rotating wheel, the first rotating wheel and the second rotating wheel are both connected with tensioning springs, and a gap is reserved between the first rotating wheel and the second rotating wheel and between the second rotating wheel and the rotating wheel.
The sample tube rotating motor drives the rotating wheel to rotate, the sample tube is clamped between the rotating wheel and two rotating wheels (respectively connected with a spring), the rotating wheel rotates slowly under the combined action of the rotating wheel and the pressure of the two rotating wheels under the action of the spring, when the sample tube rotates to the condition that the bar code faces the sample code scanner, the sample code scanner stops working after the code scanning, and the sample tube continues to be driven by the code scanning motor to move.
And a gap is reserved between the first rotating wheel and the second rotating wheel, and after the rotating wheels act, the sample tube is screwed into the space among the first rotating wheel, the second rotating wheel and the rotating wheels and clamped by the tensioning spring to rotate until the sample code scanner successfully scans codes.
Preferably, a reagent card code scanner is arranged in the bracket, the reagent card code scanner is positioned above the reagent card detection track, and a sampling needle cleaning assembly is also arranged in the bracket.
The reagent card code scanning device is used for scanning a reagent card positioned on a reagent card detection track, acquiring reagent card information and confirming that a sample detection item is correct, and the sampling needle cleaning assembly is used for cleaning a sampling needle.
The diluting cup stirring device comprises a support, a diluting cup bin, a diluting cup stirring hand driving wheel, a diluting cup stirring driven wheel and a diluting cup stirring bottle, wherein a diluting cup stirring hand driving wheel is arranged in the support, a plurality of diluting cups are placed in the diluting cup bin, a magnet is arranged in the diluting cup bin, a diluting cup stirring hand is arranged at the bottom of the diluting cup bin, the diluting cup stirring hand driving wheel and the diluting cup stirring driven wheel are driven by the diluting cup stirring hand driving wheel to drive the diluting cup stirring hand to stir the diluting cup to a preset diluting position, diluting cup stirring hand belts are arranged on the diluting cup stirring hand driving wheel and the diluting cup stirring driven wheel, the diluting cup stirring hand belts are connected with diluting cup stirring hand sliding block assemblies, and diluting liquid bottles are correspondingly arranged in the support.
The dilution needle can obtain the dilution liquid from the dilution liquid bottle through the pipeline and inject the dilution liquid into the dilution cup.
Preferably, a reagent card hand for pushing and conveying the reagent card is arranged on the reagent card detection track, and drives a reagent card hand driving wheel and a reagent card hand driven wheel through a reagent card hand electric motor so as to drive the reagent card hand to push the reagent card to the incubation detection position.
The incubation device at the incubation detection position in the present invention belongs to the prior art.
Preferably, the outside of the bracket is also covered with a shell, a display screen is arranged on the shell, a disposal opening and an instrument handle are also arranged on the side face of the shell, the full-automatic fluorescence immunoassay analyzer is also provided with a built-in printer, a paper outlet of the built-in printer is arranged on the shell, and an observation window and an item scanner are also arranged on the shell.
The display screen is used for displaying content related to detection, the analyzer can be operated through the display screen (touch), the dilution cup is pushed to the discarding port to push the detected reagent card to the discarding port, the instrument handle is used for carrying the instrument, the built-in printer is used for printing out a detection result on paper, the project code scanner is used for scanning code input of a new detection project, so that later reagent card code scanning information verification is facilitated, reagent batch number is updated, information details comprise input of production validity period, reagent use validity is monitored and the like.
Another problem to be solved by the present invention is to provide a sample detection method, which includes a normal detection process, the normal detection process includes the following steps:
(1) Turning on a power switch to wait for the completion and readiness of the initialization of the instrument;
(2) After the project is imported, placing a test tube rack with a sample tube, placing a reagent card, placing a diluent cup, and inputting patient information, sample information and a project to be detected;
(3) Clicking a detection button, pushing the test tube rack to a transverse movement track, pushing the test tube rack to a sample tube code scanning position after the test tube rack is detected to be in place, starting a sample code scanning device after the sample tube exists in the hole position of the test tube rack, slowly rotating the sample tube at the same time, enabling a bar code on the sample tube to face the sample code scanning device, and uploading data after the sample code scanning device scans codes;
(4) After the sample information is checked correctly, a reagent card is pulled out from a reagent bin corresponding to a detection item, the reagent card is stirred, and after a reagent card two-dimensional code is scanned at a reagent card code scanning position, data are uploaded;
(5) After checking the information of the reagent card, pushing the reagent card to a sample adding position;
(6) The dilution liquid cup is moved to a dilution position corresponding to the sample adding position;
(7) Shifting the test tube rack to a sample tube mixing position, forking the sample tube, lifting the forked sample tube to a certain height, and enabling the sample tube to swing back and forth within a certain angle range so as to mix samples in the test tube uniformly;
(8) After the uniform mixing is completed, inserting the sample tube back into the test tube rack, and shifting the test tube rack to a sampling position;
(9) Moving the sampling needle to the position right above the sample tube, and descending the sampling needle to enable the sampling needle to be pricked into the sample tube, and extracting a sample in the sample tube through the sampling needle;
(10) After the sample sampling is completed, lifting the sampling needle, moving the sampling needle to the position right above the dilution cup, beating the sample extracted from the sampling needle into the dilution cup, and beating a certain proportion of diluent into the dilution cup from the dilution needle;
(11) After the dilution is finished, the sampling needle is moved to be right above the dilution cup, the end part of the sampling needle is lowered to the bottom surface of the dilution cup, and the diluted sample is extracted into the sampling needle;
(12) Lifting a sampling needle, moving the sampling needle to a sample adding position, descending the sampling needle to the position above a sample adding port of a reagent card on the sample adding position, and driving a sample in the sampling needle into the sample adding port of the reagent card;
(13) Pushing the reagent card after sample addition to an incubation detection position, starting a heating module, and keeping the reagent card at a set temperature for incubation;
(14) After incubation is completed, the detection assembly is moved to the position right above the reagent card, the data acquisition module is started, the light acquisition head is moved at a detection window of the reagent card, and the data acquisition module acquires scanning signals;
(15) The test tube rack is shifted to a sample outlet position, the test tube rack is pushed out to a sample outlet platform, a diluted cup after dilution is pushed to a discarding port, and a reagent card after detection is pushed to the discarding port;
(16) And after the detection program is finished, the detection result can be checked on an instrument display screen and printed by using a built-in printer.
Preferably, the emergency detection process is further included, and the emergency detection process includes the following steps:
(1) Turning on a power switch to wait for the completion and readiness of the initialization of the instrument;
(2) After the project is imported, placing a test tube rack with a sample tube, placing a reagent card, placing a diluent cup, and inputting patient information, sample information and a project to be detected;
(3) Opening an emergency sample placement position, inserting an emergency test tube into an emergency sample test tube slot, pushing back the emergency sample placement position, clicking an emergency button, and starting a detection flow after the emergency sample is detected to be inserted;
Or executing the step (3) in the emergency detection process in the normal detection process.
Drawings
The following is a further detailed description of embodiments of the invention with reference to the accompanying drawings:
FIG. 1 is a schematic diagram of the overall structure of a full-automatic fluorescence immunoassay analyzer of the present invention;
FIG. 2 is a schematic diagram of the internal structure of the fully automatic fluorescence immunoassay analyzer in FIG. 1;
FIG. 3 is a schematic diagram of a sample injection and discharge device partially shown in FIG. 2;
FIG. 4 is a schematic diagram of a sample injection pusher of the sample injection and discharge device of FIG. 3;
FIG. 5 is a schematic diagram of a sample introduction cross pushing hand of the sample introduction and discharge device in FIG. 3;
FIG. 6 is a schematic diagram of a sample ejection pusher of the sample ejection device of FIG. 3;
FIG. 7 is a construction diagram of a test tube rack;
FIG. 8 is a schematic view of a sample tube mixing assembly according to the present invention;
FIG. 9 is a schematic view of the sample tube mixing assembly of FIG. 8 laid flat;
FIG. 10 is a schematic view of a sample needle motion assembly of the present invention;
FIG. 11 is a schematic rear view of the sample needle motion assembly of FIG. 10;
FIG. 12 is a schematic view of the mechanism of the reagent cartridge of the present invention;
FIG. 13 is a schematic view in partial cross-section of a side of the reagent cartridge mechanism of FIG. 12;
FIG. 14 is a schematic view of the structure of a single cartridge of the cartridge mechanism of FIG. 12;
FIG. 15 is a schematic view of the optical path detecting unit according to the present invention;
FIG. 16 is a schematic view of a part of the optical path detecting unit shown in FIG. 15;
FIG. 17 is a schematic diagram of a sample feeding and discharging platform structure;
FIG. 18 is a schematic diagram of the sample introduction cross pushing hand structure in the full-automatic fluorescence immunoassay analyzer of the present invention;
FIG. 19 is a schematic diagram of a code scanning rotation mechanism;
FIG. 20 is a schematic top view of the code scanning rotation mechanism of FIG. 19;
FIG. 21 is a schematic view of the position structure of the rack detection sensor, rack positioner and sample scanner;
FIG. 22 is a schematic view of the partial structure of FIG. 2;
FIG. 23 is a schematic view of the partially enlarged construction of FIG. 2;
FIG. 24 is a schematic view of the construction of a reagent cartridge hand in the reagent cartridge mechanism;
FIG. 25 is a schematic view of the reagent cartridge hand of FIG. 24 in an initial position;
FIG. 26 is a schematic view showing the structure of the washing tank in a partially enlarged manner in FIG. 2;
FIG. 27 is a schematic view of the partially enlarged view of the cleaning tank of FIG. 2 in a top view;
FIG. 28 is a schematic view of the reagent card hand and dilution cup hand shown in FIG. 2 partially enlarged;
FIG. 29 is a schematic view of the side partial enlarged construction of FIG. 2;
FIG. 30 is a schematic view of the dilution cup structure;
FIG. 31 is a schematic view of the bottom of the fully automated fluorescence immunoassay apparatus of the present invention in a partial bottom view;
FIG. 32 is a schematic view of the construction of the mounting position of the incubator;
FIG. 33 is a schematic top view of a fully automated fluorescence immunoassay apparatus of the present invention;
FIG. 34 is a schematic view of a structure of a state of movement of the traverse pushing hand;
wherein, the device comprises a 1-bracket, a 101-vertical plate, a 2-base, a 3-sample injection, The sample discharging device, 301-sample feeding pushing hand structure, 3011-sample feeding pushing hand, 3012-slide block assembly I, 3013-guide rail I, 3014-belt I, 3015-motor I, 3016-driving wheel I, 3017-driven wheel I, 302-sample feeding transverse pushing hand structure, 3021-transverse pushing hand, 3022-slide block assembly II, 3023-guide rail II, 3024-belt II, 3025-motor II, 3026-driving wheel II, 3027-driven wheel II, 303-sample discharging pushing hand structure, 3031-sample discharging pushing hand, 3032-slide block assembly III, 3033-guide rail III, 3034-belt III, 3035-motor III, 3036-driving wheel III, 3037-driven wheel III, 304-sample feeding platform, 305-transverse moving track, 306-sample discharging platform, 4-sample tube, 5-sample tube mixing assembly, 501-swinging arm, 502-swinging motor, 503-horizontal sliding block component, 504-horizontal motor, 505-horizontal guide rail, 506-up-down fixed block, 507-up-down sliding block component, 508-up-down guide rail, 509-up-down motor, 5010-up-down motion optocoupler, 5011-up-down motion sensing piece, 5012-horizontal motion optocoupler, 5013-horizontal motion sensing piece, 5014-shrapnel clip, 6-sampling needle motion component, 601-sampling needle, 602-sampling needle up-down motion component, 6021-sampling needle up-down motion motor, 6022-sampling needle up-down motion sliding block component, 6023-sampling needle up-down guide rail, 6024-sampling needle up-down motion motor lead screw, 6025-sampling needle up-down motion limiting optocoupler, 603-sampling needle horizontal movement components, 6031-sampling needle horizontal movement motors, 6032-sampling needle horizontal movement sliding block components, 6033-sampling needle horizontal guide rails, 6034-sampling needle horizontal movement driving wheels, 6035-sampling needle horizontal movement belts, 6036-sampling needle horizontal movement driven wheels, 6037-sampling needle horizontal movement limiting photocouplers, 604-fixed vertical plates, 605-guiding bending belts, 606-sampling needle movement component supporting frames, 607-dilution needles, 7-reagent bin mechanisms, 701-reagent cards, 702-reagent bin supports, 7021-upper pressing plates, 7022-reagent bin grooves, 7023-fixed plates, 703-reagent bins, 7031-reagent bin handles, 704-reagent bin hand-pulling devices, 7041-reagent bin hand-pulling motors, 7042-hand-pulling driving wheels, 7043-a hand-pulling driven wheel, 7044-a hand-pulling belt, 7045-a hand-pulling sliding block component, 7046-a hand-pulling guide rail, 7047-a reagent cabin hand-pulling head, 7048-a hand-pulling head, 7049-an elastic piece, 70410-a hand-pulling tail, 70411-an inclined part, 705-a reagent card detection track, 706-a reagent card detector, 708-a fixed block, 709-a magnet, 7010-a reagent cabin pushing in-place sensor, 8-an optical path detection component, 801-an optical path detection component fixed block, 8011-a window, 802-an optical path detection component sliding block component, 803-an optical path detection component translation guide rail, 804-an optical path detection component translation motor, 805-a lighting head component, 8051-a shielding cover, 8052-a lighting head, 806-lighting head motion motor, 807-lighting head sliding block component, 808-detection component guiding bending belt, 9-test tube rack, 901-test tube rack positioning groove, 10-emergency sample placing position, 11-emergency position, 12-emergency sample tube detection sensor, 13-test tube rack detection sensor, 14-test tube rack positioning device, 15-sample tube detection sensor, 16-sample scanner, 17-code scanning rotating mechanism, 1701-sample tube rotating motor, 1702-rotating wheel, 1703-rotating wheel one, 1704-rotating wheel two, 1705-tensioning spring, 18-reagent card scanner, 19-sampling needle cleaning component, 20-dilution cup bin, 2001-dilution cup, 2002-dilution cup hand, 2003-dilution cup hand-pulling electric machine, 2004-dilution cup hand-pulling wheel, 2005-dilution cup hand-pulling wheel, 2006-dilution cup hand-pulling belt, 2007-dilution cup hand-pulling sliding block component, 2008-dilution liquid bottle, 21-reagent card hand-pulling wheel, 22-reagent card hand-pulling motor, 23-reagent card hand-pulling wheel, 24-reagent card hand-pulling wheel, 25-housing, 26-display window, 27-sample window, 29-dilution cup hand-cleaning device, sample placing window, 32-sample detecting window, and 32-detecting instrument.
Detailed Description
As shown in fig. 1 and 2, the full-automatic fluorescence immunoassay analyzer of the embodiment comprises a support 1, wherein the support 1 is arranged on a base 2, a sample feeding and discharging device 3 is arranged on the base 2, the sample feeding and discharging device 3 is used for pushing a sample tube 4 into a sample feeding device to detect and pushing the sample tube 4 out of the sample, a sample tube mixing assembly 5 is arranged on the side face of the sample feeding and discharging device 3, the sample tube mixing assembly 5 is used for mixing samples in the sample tube 4, the sample tube 4 is pushed into the sample feeding device 3 by the sample feeding device to detect and pushing out the samples, a sample needle moving assembly 6 is used for extracting the samples in the sample tube 4, a sample needle 601 is arranged on the sample needle moving assembly 6, as shown in fig. 10, a reagent bin mechanism 7 is arranged in the support 2, a plurality of reagent cards 701 are stacked in the reagent bin mechanism 7, as shown in fig. 14, the sample needle 601 is used for adding the diluted samples into a reagent card 701, and a reagent light path 8 is further arranged in the sample feeding device 2.
The full-automatic fluorescence immunoassay analyzer is characterized in that a shell 25 is further covered outside the support 1, a display screen 26 is arranged on the shell 25, content related to detection can be checked on the display screen 26, a disposal port 27 and an instrument gripper 28 are further arranged on the side face of the shell 25, a built-in printer 29 is further arranged on the shell 25, a viewing window 30 and an item scanner 31 are further arranged on the shell 25, a reagent card scanner 18 (shown in fig. 23) is arranged in the support 1, the reagent card scanner 18 is arranged above a reagent card detection track 705, a sampling needle cleaning assembly 19 is further arranged in the support 1, when the sampling needle cleaning assembly 19 cleans a sampling needle 601, the inner wall of the needle enters the sampling needle 601 by a cleaning system and is washed by a side inlet, waste liquid is discharged by a lower discharge port through a liquid discharging system, a normal sample sampling port 33 and an emergency sample sampling port 32 are arranged on a partition plate connected with the sampling needle cleaning assembly 19, and the sampling needle cleaning assembly 19 is prevented from being pulled out after the sampling needle is prevented from being pricked by a rubber cap 27, and the sampling needle is prevented from being pulled out after the sampling needle is plugged into the sample cap as shown in fig. 27.
As shown in fig. 3-6, the sample feeding and discharging device 3 comprises a sample feeding pushing structure 301, a sample feeding transverse pushing structure 302, a sample discharging pushing structure 303 and a transverse moving track 305 (as shown in fig. 17), wherein the sample feeding pushing structure 301 is used for pushing a sample tube 4 placed on a sample feeding platform 304 into sample feeding longitudinally, the sample feeding transverse pushing structure 302 is used for pushing the sample tube 4 into sample feeding transversely for detection, and the sample discharging pushing structure 303 is used for pushing the detected sample tube 4 out to a sample discharging platform 306 for sample discharging, as shown in fig. 17.
Specifically, as shown in fig. 4, the sample pushing structure 301 has a sample pushing handle 3011, where the sample pushing handle 3011 is disposed on a guide rail one 3013 through a slide block component one 3012, the slide block component one 3012 is fixed on a belt one 3014, and a motor one 3015 drives the slide block component one 3012 to reciprocate longitudinally along the guide rail one 3013 through a driving wheel one 3016, a driven wheel one 3017 and the belt one 3014, so as to switch, and further push a sample tube 4 (located on a test tube rack 9) into sample in the longitudinal direction.
The sample tube 4 (on the test tube rack 9) of the sample is pushed into the sample pushing hand 3011 and enters the sample tube rack 9, the test tube rack 9 is pressed by the test tube rack 9 along the transverse moving track 305 to perform sample feeding and sample discharging, as shown in fig. 5, the sample pushing hand structure 302 is provided with a plurality of transverse pushing hands 3021, three sample pushing hands are arranged in the embodiment, the three sample pushing hands are all the same in the embodiment, the sample pushing hands include a pushing hand head part, a pushing hand tail part and an inclined part, when the transverse pushing hands 3021 transversely push the test tube rack 9, the pushing hand head part faces the pushing direction, in this way, when the transverse pushing hands 3021 move backwards, the bottom surface of the test tube rack 9 is depressed, so that the pushing hands 7048 can be sprung up again to push the test tube rack 9 to move after being pressed down, as shown in fig. 18, 24 and 34, the transverse pushing hands 3021 are arranged on the guide rail two 3023 through a slide block assembly two 3022, the slide block assembly two 3022 is fixed on the belt two 3024, a motor two driven wheel 3025 is arranged on the driven wheel 3026, the two driven wheel 3027 and the slide block 3027 is arranged on the guide rail two sample tube rack 9, and the sample tube rack 9 is driven by the two driving wheel 3027 and the two sample pushing hands are positioned along the guide rail 9, and the sample pushing device is positioned on the horizontal tube rack 9, and the sample pushing device is positioned along the guide rail 9, and the sample pushing device is positioned between the two sample pushing device is positioned on the sample tube rack 9 and has the sample pushing device.
As shown in fig. 6, the sample pushing structure 303 is provided with a sample pushing handle 3031, the sample pushing handle 3031 is arranged on a guide rail three 3033 through a sliding block assembly three 3032, the sliding block assembly three 3032 is fixed on a belt three 3034, and a motor three 3035 drives the sliding block assembly three 3032 to longitudinally reciprocate along the guide rail three 3033 through a driving wheel three 3036, a driven wheel three 3037 and the belt three 3034, so that the detected sample tube 4 is pushed out to the sample discharging platform 306 for sample discharging, as shown in fig. 17.
As shown in fig. 8 and 9, the sample tube mixing assembly 5 is provided with a swing arm 501 for swinging the sample tube 5, the swing arm 501 is connected with a rotating shaft of a swing motor 502 to drive the sample tube 4 to swing left and right, the swing motor 502 is connected with a horizontal slide block assembly 503, the horizontal slide block assembly 503 is driven by a horizontal motor 504 to move horizontally along a horizontal guide rail 505, the horizontal motor 504 is connected with an upper and lower fixing block 506, the upper and lower fixing block 506 is connected with an upper and lower slide block assembly 507, the upper and lower slide block assembly 507 is arranged on an upper and lower guide rail 508, and the upper and lower fixing block 506 is driven by an upper and lower motor 509 to move up and down along the upper and lower guide rail 508.
In this embodiment, the upper and lower motors 509 and the horizontal motor 504 are both screw motors, an up-and-down motion optocoupler 5010 is disposed on the side of the upper and lower motors 509, correspondingly, an up-and-down motion sensing piece 5011 is disposed on the upper and lower slider assembly 507, a horizontal motion optocoupler 5012 is disposed on the side of the horizontal motor 504, correspondingly, a horizontal motion sensing piece 5013 is disposed on the horizontal slider assembly 503, and a spring clip 5014 is disposed on the swing arm 501 for clipping the sample tube 4 to mix the sample uniformly.
The sampling needle moving assembly 6 comprises a sampling needle up-down moving assembly 602 and a sampling needle horizontal moving assembly 603, which are respectively shown in fig. 10 and 11, wherein the sampling needle up-down moving assembly 602 is provided with a sampling needle up-down moving motor 6021, the sampling needle 601 is arranged on a sampling needle up-down moving sliding block assembly 6022 in the sampling needle moving assembly 6, the sampling needle up-down moving motor 6021 drives the sampling needle up-down moving sliding block assembly 6022 to reciprocate along a sampling needle up-down guide rail 6023, the sampling needle horizontal moving assembly 603 is provided with a sampling needle horizontal moving motor 6031, the sampling needle up-down moving assembly 602 is connected with the sampling needle horizontal moving sliding block assembly 6032 through a fixed vertical plate 604, and the sampling needle horizontal moving motor 6031 drives the sampling needle horizontal moving sliding block assembly 6032 to reciprocate on the sampling needle horizontal guide rail 6033.
In this embodiment, the sampling needle up-down movement motor 6021 is a screw motor, the sampling needle up-down movement sliding block assembly 6022 is sleeved on a sampling needle up-down movement motor screw 6024 connected with the sampling needle up-down movement motor 6021, the sampling needle horizontal movement motor 6031 is a stepping motor, the sampling needle horizontal movement motor 6031 drives the sampling needle horizontal movement driving wheel 6034 to rotate, the sampling needle horizontal movement driven wheel 6036 is driven by a sampling needle horizontal movement belt 6035 sleeved on the sampling needle horizontal movement driving wheel 6034, and the fixed riser 604 is connected with the sampling needle horizontal movement belt 6035 by the sampling needle horizontal movement sliding block assembly 6032.
The needle movement assembly 6 further comprises a guiding bending strap 605, one end of the guiding bending strap 605 is fixed above the fixed riser 604, the other end of the guiding bending strap 605 is arranged on the needle movement assembly support 606, and the guiding bending strap 605 is longitudinally arranged along the needle horizontal guide rail 6033.
The sampling needle moving assembly support frame 606 is provided with a sampling needle horizontal movement limiting optocoupler 6037 at the tail part of the end close to the sampling needle horizontal guide rail 6033.
The sampling needle 601 is arranged on the sampling needle up-and-down sliding block assembly 6022, and the fixed riser 604 is also provided with a dilution needle 607.
As shown in fig. 12-14, the reagent compartment mechanism 7 comprises a reagent compartment bracket 702 arranged on the base 2, a plurality of mutually independent reagent compartments 703 are arranged on the reagent compartment bracket 702, a plurality of reagent cards 701 can be stacked in each reagent compartment 703, a plurality of mutually independent reagent compartment hand pulling devices 704 are correspondingly arranged at the bottom of each reagent compartment 703 and positioned in the reagent compartment bracket 702, and the reagent compartment hand pulling devices 704 are used for pulling the reagent cards 701 in the reagent compartments 703 to the reagent card detection tracks 705 positioned in the bracket 1, as shown in fig. 23, 28 and 33.
In fig. 13, a reagent card detector 706 is mounted on the inner side wall of the reagent chamber 703, the reagent card detector 706 is used for detecting the number of reagent cards 701 in the reagent chamber 703, a fixed block 708 corresponding to each reagent chamber 701 is further disposed on the reagent chamber bracket 702, and a magnet 709 is disposed on the reagent chamber 703 or the fixed block 708.
The reagent bin hand-pulling device 704 is provided with a reagent bin hand-pulling electric machine 7041, the reagent bin hand-pulling electric machine 7041 drives a hand-pulling sliding block assembly 7045 fixedly connected with the hand-pulling belt 7044 to reciprocate along a hand-pulling guide rail 7046 through a hand-pulling driving wheel 7042, a hand-pulling driven wheel 7043 and a hand-pulling belt 7044, and a reagent bin hand-pulling 7047 is arranged on the hand-pulling sliding block assembly 7045.
The reagent cartridge hand 7047 comprises a hand-pulling head 7048, as shown in fig. 24 and 25, an elastic piece 7049 is arranged below the hand-pulling head 7048, a common spring is adopted in the embodiment, the reagent cartridge hand 7047 further comprises a hand-pulling tail 70410 movably connected with the hand-pulling sliding block assembly 7045, the hand-pulling head 7048 is connected with the hand-pulling tail 70410 through an inclined part 70411, an upper pressing plate 7021 is arranged on the reagent cartridge support 702, the reagent cartridge hand 7047 pushes out the reagent card 701 and then moves backwards, and the hand-pulling head 7048 is pressed down due to the compression of the reagent card 701. In fig. 25, the initial position is the position when the reagent chamber hand 7047 is reset, the push-pull of the reagent chamber 703 is not affected, when the reagent chamber hand 7047 moves forward when working is required, and when the upper pressure plate 7021 is moved, the hand head 7048 pops up to push the reagent card 701 to move.
The reagent bin 703 is further provided with a reagent bin handle 7031, the fixing block 708 is provided with a reagent bin in-place sensor 7010, the reagent bin bracket 702 is further provided with a plurality of mutually independent reagent bin slots 7022, and the upper parts of the reagent bin slots 7022 are fixedly connected through a fixing plate 7023.
As shown in fig. 15 and 16, the optical path detecting component 8 is located in the bracket 1 and comprises an optical path detecting component fixing block 801, the optical path detecting component fixing block 801 is connected with an optical path detecting component sliding block component 802, the optical path detecting component sliding block component 802 is located on an optical path detecting component translation guide rail 803, the optical path detecting component 8 further comprises an optical path detecting component translation motor 804, the optical path detecting component translation motor 804 drives the optical path detecting component fixing block 801 to reciprocate along the optical path detecting component translation guide rail 803, and a light extraction head component 805 is mounted on the optical path detecting component fixing block 801.
The light path detection assembly fixing block 801 is further provided with a lighting head moving motor 806, the lighting head assembly 805 is connected with the lighting head sliding block assembly 807, and the lighting head moving motor 806 drives the lighting head assembly 805 to reciprocate along a direction perpendicular to the translation guide rail 803 of the light path detection assembly.
The lighting head assembly 805 comprises an upper shielding cover 8051 and a lower lighting head 8052, as shown in fig. 16, and the lighting head assembly 805 can move back and forth to collect light signals through a window 8011 arranged on the light path detection assembly fixing block 801, and the detection window of the reagent card 701 positioned below.
The optical path detection component fixing block 801 is connected with one end of a detection component guiding bending strap 808, and the detection component guiding bending strap 808 is arranged along the direction of the optical path detection component translation guide 803 and is positioned on the side surface of the optical path detection component translation guide 803.
The full-automatic fluorescence immunoassay analyzer of the present embodiment further includes an emergency sample placement position 10 for placing the sample tube 4, as shown in fig. 3, and an emergency sample placement position 11 and an emergency sample tube detection sensor 12 (as shown in fig. 26 and 27), where the emergency sample placement position 10 is located in the horizontal movement direction of the sampling needle 601.
As shown in fig. 19, the full-automatic fluorescence immunoassay analyzer is provided with a test tube rack detection sensor 13, a test tube rack positioner 14 and a sample tube detection sensor 15 beside the sample feeding and discharging device 3, wherein the test tube rack detection sensor 13 is mounted on a vertical plate 101 of the bracket 1, and the test tube rack positioner 14 and the sample tube detection sensor 15 sense through corresponding windows of the vertical plate 101 of the bracket 1.
The sample tube detection sensor 15 is provided with a sample code scanner 16 above, as shown in fig. 20 and 21, a code scanning rotating mechanism 17 (as shown in fig. 22) is arranged close to the sample code scanner 16, the code scanning rotating mechanism 17 comprises a sample tube rotating motor 1701, the sample tube rotating motor 1701 drives a rotating wheel 1702 to rotate, the code scanning rotating mechanism 17 further comprises a first rotating wheel 1703 and a second rotating wheel 1704, the first rotating wheel 1703 and the second rotating wheel 1704 are both connected with a tensioning spring 1705, and a gap is reserved between the first rotating wheel 1703, the second rotating wheel 1704 and the rotating wheel 1702.
The diluting cup bin 20 is further arranged in the support 1, as shown in fig. 28, 29 and 31, a plurality of diluting cups 2001 (as shown in fig. 30) are arranged in the diluting cup bin 20, one cup position is needed for one sample detection, 8 cup positions, preferably 10 cup positions, are the same as the sample tube positions of the reagent rack 9 in fig. 30, diluting cup pulling hands 2002 are arranged at the bottom of the diluting cup bin 20, the diluting cup pulling hands 2002 are driven to pull the diluting cups 2001 to a diluting position by the diluting cup pulling hand driving wheel 2004 and the diluting cup pulling hand driven wheel 2005, diluting cup pulling hand belts 2006 are arranged on the diluting cup pulling hand driving wheel 2004 and the diluting cup pulling hand driven wheel 2005, the diluting cup pulling hands 2002 are connected with the diluting cup pulling hand sliding block assembly 2007, and correspondingly, a diluting liquid bottle 2008 is further arranged in the support 1.
The reagent card detecting track 705 is provided with a reagent card hand 21 for picking up and delivering the reagent card 701, the reagent card hand 21 drives a reagent card hand driving wheel 23 and a reagent card hand driven wheel 24 through a reagent card hand electric machine 22 so as to drive the reagent card hand 21 to pick up the reagent card 701 to an incubation detecting position 34, as shown in fig. 32, the incubation device performs incubation reactions on the reagent card 701 for different times according to different projects below a reagent card detecting platform in the movement range of the optical path detecting assembly 8, the lighting head 8052 detects optical signals, and the data processing module collects, converts (optical signal-electrical signal), amplifies and the like the optical signals to obtain detecting results.
The sample detection method provided by the invention comprises a normal detection flow, wherein the normal detection flow comprises the following steps of:
(1) Turning on a power switch to wait for the completion and readiness of the initialization of the instrument;
(2) After the project is imported, placing a test tube rack with a sample tube, placing a reagent card, placing a diluent cup, and inputting patient information, sample information and a project to be detected;
(3) Clicking a detection button, pushing the test tube rack to a transverse movement track, pushing the test tube rack to a sample tube code scanning position after the test tube rack is detected to be in place, starting a sample code scanning device after the sample tube exists in the hole position of the test tube rack, slowly rotating the sample tube at the same time, enabling a bar code on the sample tube to face the sample code scanning device, and uploading data after the sample code scanning device scans codes;
(4) After the sample information is checked correctly, a reagent card is pulled out from a reagent bin corresponding to a detection item, the reagent card is stirred, and after a reagent card two-dimensional code is scanned at a reagent card code scanning position, data are uploaded;
(5) After checking the information of the reagent card, pushing the reagent card to a sample adding position;
(6) The dilution liquid cup is moved to a dilution position corresponding to the sample adding position;
(7) Shifting the test tube rack to a sample tube mixing position, forking the sample tube, lifting the forked sample tube to a certain height, and enabling the sample tube to swing back and forth within a certain angle range so as to mix samples in the test tube uniformly;
(8) After the uniform mixing is completed, inserting the sample tube back into the test tube rack, and shifting the test tube rack to a sampling position;
(9) Moving the sampling needle to the position right above the sample tube, and descending the sampling needle to enable the sampling needle to be pricked into the sample tube, and extracting a sample in the sample tube through the sampling needle;
(10) After the sample sampling is completed, lifting the sampling needle, moving the sampling needle to the position right above the dilution cup, beating the sample extracted from the sampling needle into the dilution cup, and beating a certain proportion of diluent into the dilution cup from the dilution needle;
(11) After the dilution is finished, the sampling needle is moved to be right above the dilution cup, the end part of the sampling needle is lowered to the bottom surface of the dilution cup, and the diluted sample is extracted into the sampling needle;
(12) Lifting a sampling needle, moving the sampling needle to a sample adding position, descending the sampling needle to the position above a sample adding port of a reagent card on the sample adding position, and driving a sample in the sampling needle into the sample adding port of the reagent card;
(13) Pushing the reagent card after sample addition to an incubation detection position, starting a heating module, and keeping the reagent card at a set temperature for incubation;
(14) After incubation is completed, the detection assembly is moved to the position right above the reagent card, the data acquisition module is started, the light acquisition head is moved at a detection window of the reagent card, and the data acquisition module acquires scanning signals;
(15) The test tube rack is shifted to a sample outlet position, the test tube rack is pushed out to a sample outlet platform, a diluted cup after dilution is pushed to a discarding port, and a reagent card after detection is pushed to the discarding port;
(16) And after the detection program is finished, the detection result can be checked on an instrument display screen and printed by using a built-in printer.
The emergency detection method also comprises an emergency detection process, wherein the emergency detection process comprises the following steps of:
(1) Turning on a power switch to wait for the completion and readiness of the initialization of the instrument;
(2) After the project is imported, placing a test tube rack with a sample tube, placing a reagent card, placing a diluent cup, and inputting patient information, sample information and a project to be detected;
(3) Opening an emergency sample placement position, inserting an emergency test tube into an emergency sample test tube slot, pushing back the emergency sample placement position, clicking an emergency button, and starting a detection flow after the emergency sample is detected to be inserted;
Or executing the step (3) in the emergency detection process in the normal detection process.
Specifically, the steps of sample detection using the full-automatic fluorescence immunoassay analyzer in this embodiment are:
the normal detection flow comprises (1) turning on a power switch, waiting for the initialization of the instrument to be completed and ready;
(2) After the project is introduced, placing a test tube rack 9 with a sample tube 4 on a sample introduction platform 304, placing a reagent card 701 in a reagent bin 703, placing a dilution cup 2001 in a dilution liquid bin 20, and inputting patient information, sample information and a project to be detected;
(3) Clicking a detection button, pushing the test tube rack 9 to a transverse movement track 305 by a sample pushing hand, pushing the test tube rack 9 to a sample tube code scanning position by a sample transverse pushing hand 3021 after the test tube rack detection sensor 13 detects that the test tube rack 9 is in place, starting a sample code scanner 16 after the sample tube detection sensor 15 detects that the sample tube rack 9 has the sample tube 4 in the hole site, starting a sample tube rotating motor 1701, slowly rotating the sample tube 4, enabling a bar code on the sample tube 4 to face the sample code scanner 16, scanning the code by the sample code scanner 16, uploading the code to an upper computer, and uploading data;
(4) After the sample information is checked correctly, a reagent card 701 is pulled out onto a reagent card detection track 705 from a reagent bin 703 corresponding to a detection item by a reagent bin pulling hand 7047, the reagent card 701 is pulled by a reagent card pulling hand 21, and a reagent card two-dimensional code is scanned by a reagent card code scanner 18 at a reagent card code scanning position and is uploaded to an upper computer for long data transmission;
(5) After the reagent card information is checked correctly, the reagent card pulling hand 21 pushes the reagent card 701 to a sample adding position;
(6) The dilution cup pulling hand 2002 pulls the dilution cup 2001 from the dilution cup holder 20 to a dilution position corresponding to the sample loading position;
(7) The sample injection transverse hand 3021 dials a test tube rack 9 to a sample tube mixing position (a sample tube mixing assembly 5), a mixing horizontal movement motor (namely a horizontal motor 504) pushes a test tube spring clip 5014 forward to fork the sample tube 4, a mixing up-down movement motor (namely an up-down motor 509) lifts the spring clip 5014 which fork the sample tube 4 by a certain height, and a mixing swing motor 502 enables the sample tube spring clip 5014 to swing back and forth within a certain angle range so as to mix samples in the sample tube 4;
(8) After the mixing is completed, the mixing swing motor 502 is reset, the mixing up-down motion motor 509 descends the sample tube elastic clamp 5014 to enable the sample tube 4 to be inserted back into the test tube rack 9, the mixing horizontal motion motor 504 retracts the sample tube elastic clamp 5014, and the sample injection transverse poking hand 3021 pokes the test tube rack 9 to a sampling position;
(9) The sampling needle horizontal movement motor 6031 moves the sampling needle 601 to the position right above the sample tube 4, the sampling needle lifting motor (i.e. the sampling needle up-down movement motor 6021) descends the sampling needle 601, so that the sampling needle 601 is pricked into the sample tube 4, and the sampling pump is connected with the sampling needle 601 through a pipeline to extract a sample in the sample tube 4;
(10) After the sample is sampled, a sampling needle lifting motor 6021 lifts a sampling needle 601, a sampling needle horizontal movement motor 6031 moves the sampling needle 601 to be right above a dilution cup 2001, a sampling pump pumps the sample extracted from the sampling needle 601 into the dilution cup 2001, and a dilution pump pumps a certain proportion of dilution liquid into the dilution cup 2001 from the dilution needle 607 through a pipeline;
(11) After the dilution is completed, the sampling needle horizontal movement motor 6031 moves the sampling needle 601 to be right above the dilution cup 2001, the sampling needle lifting motor 6021 descends the end part of the sampling needle 601 to the bottom surface of the dilution cup 2001, and the sampling pump pumps the diluted sample into the sampling needle 601 through a pipeline;
(12) The sampling needle lifting motor 6021 lifts the sampling needle 601, the sampling needle 601 is moved to a sample adding position by the sampling needle horizontal movement motor 6031, the sampling needle lifting motor 6021 descends the sampling needle 601 to the position above a sample adding port of a reagent card on the sample adding position, and a sample in the sampling needle 601 is driven into the sample adding port of the reagent card 701;
(13) The reagent card pulling hand 21 pushes the reagent card 701 with the sample added to the incubation detection position, a heating module in the incubation device is started, and the reagent card 701 is kept at the set temperature for incubation;
(14) After incubation is completed, a detection translation motor (namely a light path detection component translation motor 804) moves a detection component (namely a light path detection component 8) to be right above the reagent card 701, a data acquisition module is started, a lighting head movement motor 806 moves a lighting head 8052 in a reagent card detection window, and the data acquisition module acquires scanning signals;
(15) Sample injection horizontal pushing hand 3021 dials test tube rack 9 to a sample outlet position, sample outlet pushing hand 3031 pushes test tube rack 9 out to sample outlet platform 306, dilution cup dialing hand 2002 pushes diluted dilution cup 2001 to disposal port 27, and reagent card dialing hand 21 pushes detected reagent card 701 to disposal port 27;
(16) The test procedure is completed and the test results can be viewed on the instrument display 26 and printed using the built-in printer.
The emergency detection flow comprises the following steps:
(1) Turning on a power switch to wait for the completion and readiness of the initialization of the instrument;
(2) After the project is imported, placing a test tube rack 9 with a sample tube 4 on a sample injection platform 304, placing a reagent card 701 in a reagent bin 703, placing a diluent cup 2001 in a diluent bin 20, and inputting patient information, sample information and a project to be detected;
(3) Opening the emergency sample placement position, inserting an emergency test tube into an emergency sample test tube slot, pushing back the emergency sample placement position 10, clicking an emergency button 11, and starting a detection flow after the emergency sample tube detection sensor 12 detects that the emergency sample is inserted. The sample injection pushing hand 3011 pushes the test tube rack 9 to the transverse movement track 305, and the normal detection process steps (4) - (14) and (16) are followed.
Or executing the step (3) in the emergency detection process in the normal detection process.
It should be noted that, in this embodiment, the sample loading position of the reagent card is aligned with the sampling position, the dilution position and the cleaning position of the sample tube, as shown in fig. 33. The reagent card scanning code position is arranged between the reagent bin and the reagent sample adding position. The reagent card detecting positions are arranged in sequence (left arrangement) along with the increase of the reagent cards, wherein the reagent card detecting positions are arranged at the farthest position (right) preferentially in the moving range of the detecting assembly.
The foregoing has outlined and described the basic principles, features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (33)
1. The full-automatic fluorescence immunoassay analyzer comprises a bracket, wherein the bracket is arranged on a base, and is characterized in that a sample injection device and a sample discharge device are arranged on the base, and the sample injection device and the sample discharge device are used for pushing a sample tube into a sample injection for detection and pushing the sample tube out for sample discharge;
The side of the sample injection and sample discharge device is provided with a sample tube mixing assembly which is used for mixing samples in the sample tube uniformly, wherein the sample tube is pushed into the sample injection by the sample injection and sample discharge device for detection and pushing out for sample discharge;
The sampling needle moving assembly is used for extracting samples in the sample tube, and is provided with a sampling needle;
a reagent bin mechanism is arranged in the bracket, a plurality of reagent cards are stacked in the reagent bin mechanism, and the sampling needle adds diluted samples to a sample adding port of the reagent cards;
the support is also internally provided with an optical path detection component for detecting the reagent card;
the reagent card sample adding position is in the same straight line with the sampling position, the dilution position and the cleaning position of the sample tube;
The diluting cup stirring device comprises a support, a diluting cup bin, a diluting cup stirring hand, a diluting cup stirring driving wheel, a diluting cup stirring driven wheel and a diluting cup stirring bottle, wherein a diluting cup stirring driving wheel is arranged in the support, a plurality of diluting cups are placed in the diluting cup bin, a diluting cup stirring hand is arranged at the bottom of the diluting cup bin, the diluting cup stirring driving wheel and the diluting cup stirring driven wheel are driven by the diluting cup stirring electric machine to drive the diluting cup stirring hand to stir the diluting cup to a diluting position, diluting cup stirring belts are arranged on the diluting cup stirring driving wheel and the diluting cup stirring driven wheel, the diluting cup stirring belts are connected with diluting cup stirring sliding block assemblies, the diluting cup stirring hand is connected with the diluting cup stirring sliding block assemblies, and a diluting liquid bottle is correspondingly arranged in the support.
2. The full-automatic fluorescence immunoassay analyzer of claim 1, wherein the sample feeding and discharging device comprises a sample feeding pushing structure, a sample feeding transverse pushing structure and a sample discharging pushing structure, wherein the sample feeding pushing structure is used for pushing a sample tube placed on a sample feeding platform into sample feeding longitudinally, the sample feeding transverse pushing structure is used for pushing the sample tube into sample feeding transversely for detection, and the sample discharging pushing structure is used for pushing the detected sample tube to a sample discharging platform for sample discharging.
3. The full-automatic fluorescence immunoassay analyzer according to claim 2, wherein the sample injection pushing arm structure is provided with a sample injection pushing arm, the sample injection pushing arm is arranged on the guide rail through a sliding block assembly I, the sliding block assembly I is fixed on a belt I, and the motor I drives the sliding block assembly I to longitudinally reciprocate along the guide rail through a driving wheel I, a driven wheel I and the belt I, so that the sample tube is pushed into the sample injection along the longitudinal direction.
4. The full-automatic fluorescence immunoassay analyzer according to claim 2, wherein the sample ejection pushing hand structure is provided with a sample ejection pushing hand, the sample ejection pushing hand is arranged on a guide rail III through a sliding block assembly III, the sliding block assembly III is fixed on a belt III, and a motor III drives the sliding block assembly III to longitudinally reciprocate along the guide rail III through a driving wheel III, a driven wheel III and the belt III, so that a detected sample tube is pushed out to the sample ejection platform for sample ejection.
5. The full-automatic fluorescence immunoassay analyzer according to any one of claims 2 to 4, wherein the sample injection transverse pushing hand structure is provided with a plurality of transverse pushing hands, the transverse pushing hands are arranged on a guide rail II through a slide block assembly II, the slide block assembly II is fixed on a belt II, a motor II drives the slide block assembly II to transversely reciprocate along the guide rail II through a driving wheel II, a driven wheel II and the belt II so as to push a sample tube into the sample injection along the transverse direction for detection, the sample tube is placed on a test tube rack with a plurality of test tube rack locating grooves, and the distance between two adjacent transverse pushing hands is not larger than the length of the test tube rack.
6. The full-automatic fluorescence immunoassay analyzer of claim 5, wherein the sample feeding and discharging device further comprises a transverse movement track, and the test tube rack performs sample feeding and discharging along the transverse movement track.
7. The full-automatic fluorescence immunoassay analyzer according to claim 1, wherein the sample tube mixing assembly is provided with a swing arm for swinging the sample tube, and the swing arm is connected with a rotating shaft of a swing motor to drive the sample tube to swing left and right, so as to mix samples in the sample tube.
8. The full-automatic fluorescence immunoassay analyzer according to claim 7, wherein the swing motor is connected with a horizontal slide block assembly, the horizontal slide block assembly is driven by the horizontal motor to move horizontally along a horizontal guide rail, the horizontal motor is connected with an upper and a lower fixing blocks, the upper and the lower fixing blocks are connected with an upper and a lower slide block assembly, the upper and the lower slide block assemblies are arranged on the upper and the lower guide rails, and the upper and the lower fixing blocks are driven by the upper and the lower motor to move up and down along the upper and the lower guide rails.
9. The full-automatic fluorescence immunoassay analyzer of claim 8, wherein the upper motor, the lower motor and the horizontal motor are all screw rod motors, an up-down motion optocoupler is arranged on the side face of the upper motor, the lower motor and the horizontal motor, correspondingly, an up-down motion sensing sheet is arranged on the upper sliding block component and the lower sliding block component, a horizontal motion optocoupler is arranged on the side face of the horizontal motor, correspondingly, a horizontal motion sensing sheet is arranged on the horizontal sliding block component.
10. The full-automatic fluorescence immunoassay analyzer according to claim 8, wherein the swing arm is provided with a spring clip for clipping the sample tube for sample mixing.
11. The full-automatic fluorescence immunoassay analyzer of claim 1, wherein the sampling needle moving assembly comprises a sampling needle up-down moving assembly and a sampling needle horizontal moving assembly, wherein the sampling needle up-down moving assembly is provided with a sampling needle up-down moving motor, the sampling needle is arranged on a sampling needle up-down moving sliding block assembly in the sampling needle moving assembly, the sampling needle up-down moving motor drives the sampling needle up-down moving sliding block assembly to reciprocate along a sampling needle up-down guide rail, the sampling needle horizontal moving assembly is provided with a sampling needle horizontal moving motor, the sampling needle up-down moving assembly is connected with the sampling needle horizontal moving sliding block assembly through a fixed vertical plate, and the sampling needle horizontal moving motor drives the sampling needle horizontal moving sliding block assembly to reciprocate on the sampling needle horizontal guide rail.
12. The full-automatic fluorescence immunoassay analyzer according to claim 11, wherein the sampling needle up-and-down movement motor is a screw motor, the sampling needle up-and-down movement sliding block assembly is sleeved on a sampling needle up-and-down movement motor screw connected with the sampling needle up-and-down movement motor, the sampling needle horizontal movement motor is a stepping motor, the sampling needle horizontal movement motor drives a sampling needle horizontal movement driving wheel to rotate, a sampling needle horizontal movement driven wheel is driven by a sampling needle horizontal movement belt sleeved on the sampling needle horizontal movement driving wheel, and the fixed riser is connected with the sampling needle horizontal movement belt by the sampling needle horizontal movement sliding block assembly.
13. The fully automatic fluoroimmunoassay instrument of claim 12, wherein the needle movement assembly further comprises a guide bending strap, one end of the guide bending strap is fixed above the fixed riser, the other end of the guide bending strap is disposed on the needle movement assembly support frame, and the guide bending strap is disposed longitudinally along the needle horizontal rail.
14. The full-automatic fluorescence immunoassay analyzer of claim 13, wherein the sampling needle up-down movement limiting optocoupler is arranged on the fixed vertical plate and positioned at the upper end of the sampling needle up-down guide rail, and the sampling needle horizontal movement limiting optocoupler is arranged on the sampling needle movement assembly support frame and positioned at the tail part of the end close to the sampling needle horizontal guide rail.
15. The fully automatic fluoroimmunoassay instrument of claim 14, wherein the sampling needle is disposed on the sampling needle up-and-down motion slide block assembly, and a dilution needle is further disposed on the fixed riser.
16. The full-automatic fluorescence immunoassay analyzer of claim 1, wherein the reagent bin mechanism comprises a reagent bin support arranged on the base, a plurality of mutually independent reagent bins are arranged on the reagent bin support, a plurality of reagent cards can be stacked in each reagent bin, a plurality of mutually independent reagent bin hand pulling devices are correspondingly arranged at the bottom of each reagent bin and in the reagent bin support, and the reagent bin hand pulling devices are used for pulling reagent cards in the reagent bins to a reagent card detection track in the support.
17. The full-automatic fluorescence immunoassay instrument according to claim 16, wherein a reagent card detector is mounted on the inner side wall of the reagent cartridge, the reagent card detector is used for detecting the number of reagent cards, a fixed block corresponding to each reagent cartridge is further arranged on the reagent cartridge support, and a magnet is arranged on the reagent cartridge or the fixed block.
18. The full-automatic fluorescence immunoassay analyzer of claim 16, wherein the reagent cartridge hand-pulling device is provided with a reagent cartridge hand-pulling machine, the reagent cartridge hand-pulling machine drives a hand-pulling sliding block assembly fixedly connected with the hand-pulling belt to reciprocate along a hand-pulling guide rail through a hand-pulling driving wheel, a hand-pulling driven wheel and a hand-pulling belt, and the hand-pulling sliding block assembly is provided with a reagent cartridge hand-pulling device.
19. The full-automatic fluorescence immunoassay analyzer according to claim 18, wherein the reagent cartridge hand includes a hand-pulling head portion, an elastic member is disposed below the hand-pulling head portion, the reagent cartridge hand further includes a hand-pulling tail portion movably connected to the hand-pulling slider assembly, the hand-pulling head portion is connected to the hand-pulling tail portion through an inclined portion, and an upper pressing plate is disposed on the reagent cartridge holder.
20. The full-automatic fluorescence immunoassay instrument according to claim 19, wherein the reagent cartridge is further provided with a reagent cartridge handle, and the fixing block is provided with a reagent cartridge in-place sensor.
21. The full-automatic fluorescence immunoassay instrument according to any one of claims 16 to 20, wherein the reagent cartridge support is further provided with a plurality of mutually independent reagent cartridge slots, and the upper parts of the reagent cartridge slots are fixedly connected through a fixing plate.
22. The full-automatic fluorescence immunoassay analyzer of claim 1, wherein the optical path detection assembly is located in the support and comprises an optical path detection assembly fixing block, the optical path detection assembly fixing block is connected with an optical path detection assembly sliding block assembly, the optical path detection assembly sliding block assembly is located on an optical path detection assembly translation guide rail, the optical path detection assembly further comprises an optical path detection assembly translation motor, the optical path detection assembly translation motor drives the optical path detection assembly fixing block to reciprocate along the optical path detection assembly translation guide rail, and the optical pickup head assembly is mounted on the optical path detection assembly fixing block.
23. The full-automatic fluorescence immunoassay analyzer according to claim 22, wherein the light path detecting assembly fixing block is further provided with a lighting head moving motor, the lighting head assembly is connected with the lighting head sliding block assembly, and the lighting head moving motor drives the lighting head assembly to reciprocate along a direction perpendicular to the translation guide rail of the light path detecting assembly.
24. The full-automatic fluorescence immunoassay analyzer of claim 23, wherein the light head assembly comprises an upper shield and a lower light head, and the light head assembly can move back and forth to collect light signals through a window arranged on the light path detection assembly fixing block and a reagent card detection window positioned below.
25. The fully automatic fluorescence immunoassay instrument according to claim 24, wherein the optical path detecting member fixing block is connected to one end of the detecting member guiding bending strap, and the detecting member guiding bending strap is disposed along the optical path detecting member translation rail direction and is located at a side surface of the optical path detecting member translation rail.
26. The full-automatic fluoroimmunoassay analyzer according to any one of claims 1 to 3, 7 to 20, 22 to 24, further comprising an emergency sample placement site for placing a sample tube and provided with an emergency site and an emergency sample tube detection sensor, wherein the emergency sample placement site is located in a horizontal movement direction of the sampling needle.
27. The full-automatic fluorescence immunoassay analyzer of claim 26, wherein the full-automatic fluorescence immunoassay analyzer is provided with a tube rack detection sensor, a tube rack positioner and a sample tube detection sensor at the rear side of the sample introduction and discharge device, wherein the tube rack detection sensor is mounted on a riser of the support, and the tube rack positioner and the sample tube detection sensor sense through corresponding windows of the riser of the support.
28. The full-automatic fluorescence immunoassay analyzer of claim 27, wherein a sample code scanner is arranged above the sample tube detection sensor, a code scanning rotating mechanism is arranged close to the sample code scanner and comprises a sample tube rotating motor, the sample tube rotating motor drives a rotating wheel to rotate, the code scanning rotating mechanism further comprises a first rotating wheel and a second rotating wheel, the first rotating wheel and the second rotating wheel are both connected with tensioning springs, and a gap is reserved between the first rotating wheel and the second rotating wheel and between the second rotating wheel and the rotating wheel.
29. The full-automatic fluorescence immunoassay analyzer according to any one of claims 1 to 3, 7 to 20, and 22 to 24, wherein a reagent card scanner is disposed in the holder, the reagent card scanner is disposed above the reagent card detection track, and a sampling needle cleaning assembly is further disposed in the holder.
30. The fully automatic fluorescence immunoassay analyzer according to any one of claims 1 to 3, 7 to 20, 22 to 24, wherein a reagent card hand for pushing a reagent card is provided on the reagent card detection track, and the reagent card hand drives a reagent card hand driving wheel and a reagent card hand driven wheel through a reagent card hand electric machine to drive the reagent card hand to push the reagent card to an incubation detection position.
31. The full-automatic fluorescence immunoassay analyzer according to any one of claims 1to 3,7 to 20, and 22 to 24, wherein the housing is further covered with a housing, a display screen is provided on the housing, a disposal port and an instrument gripper are further provided on a side surface of the housing, the full-automatic fluorescence immunoassay analyzer is further provided with a built-in printer, a paper outlet of the built-in printer is provided on the housing, and a viewing window and a project scanner are further provided on the housing.
32. A sample detection method using the full-automatic fluorescence immunoassay analyzer of claim 1, comprising a normal detection procedure, the normal detection procedure comprising the steps of:
(1) Turning on a power switch to wait for the completion and readiness of the initialization of the instrument;
(2) After the project is imported, placing a test tube rack with a sample tube, placing a reagent card, placing a diluent cup, and inputting patient information, sample information and a project to be detected;
(3) Clicking a detection button, pushing the test tube rack to a transverse movement track, pushing the test tube rack to a sample tube code scanning position after the test tube rack is detected to be in place, starting a sample code scanning device after the sample tube exists in the hole position of the test tube rack, slowly rotating the sample tube at the same time, enabling a bar code on the sample tube to face the sample code scanning device, and uploading data after the sample code scanning device scans codes;
(4) After the sample information is checked correctly, a reagent card is pulled out from a reagent bin corresponding to a detection item, the reagent card is stirred, and after a reagent card two-dimensional code is scanned at a reagent card code scanning position, data are uploaded;
(5) After checking the information of the reagent card, pushing the reagent card to a sample adding position;
(6) The dilution liquid cup is moved to a dilution position corresponding to the sample adding position;
(7) Shifting the test tube rack to a sample tube mixing position, forking the sample tube, lifting the forked sample tube to a certain height, and enabling the sample tube to swing back and forth within a certain angle range so as to mix samples in the test tube uniformly;
(8) After the uniform mixing is completed, inserting the sample tube back into the test tube rack, and shifting the test tube rack to a sampling position;
(9) Moving the sampling needle to the position right above the sample tube, and descending the sampling needle to enable the sampling needle to be pricked into the sample tube, and extracting a sample in the sample tube through the sampling needle;
(10) After the sample sampling is completed, lifting the sampling needle, moving the sampling needle to the position right above the dilution cup, beating the sample extracted from the sampling needle into the dilution cup, and beating a certain proportion of diluent into the dilution cup from the dilution needle;
(11) After the dilution is finished, the sampling needle is moved to be right above the dilution cup, the end part of the sampling needle is lowered to the bottom surface of the dilution cup, and the diluted sample is extracted into the sampling needle;
(12) Lifting a sampling needle, moving the sampling needle to a sample adding position, descending the sampling needle to the position above a sample adding port of a reagent card on the sample adding position, and driving a sample in the sampling needle into the sample adding port of the reagent card;
(13) Pushing the reagent card after sample addition to an incubation detection position, starting a heating module, and keeping the reagent card at a set temperature for incubation;
(14) After incubation is completed, the detection assembly is moved to the position right above the reagent card, the data acquisition module is started, the light acquisition head is moved at a detection window of the reagent card, and the data acquisition module acquires scanning signals;
(15) The test tube rack is shifted to a sample outlet position, the test tube rack is pushed out to a sample outlet platform, a diluted cup after dilution is pushed to a discarding port, and a reagent card after detection is pushed to the discarding port;
(16) And after the detection program is finished, the detection result can be checked on an instrument display screen and printed by using a built-in printer.
33. The method of claim 32, further comprising an emergency test procedure, the emergency test procedure comprising the steps of:
(1) Turning on a power switch to wait for the completion and readiness of the initialization of the instrument;
(2) After the project is imported, placing a test tube rack with a sample tube, placing a reagent card, placing a diluent cup, and inputting patient information, sample information and a project to be detected;
(3) Opening an emergency sample placement position, inserting an emergency test tube into an emergency sample test tube slot, pushing back the emergency sample placement position, clicking an emergency button, and starting a detection flow after the emergency sample is detected to be inserted;
Or executing the step (3) in the emergency detection process in the normal detection process.
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