CN111728910A - Fermented extract of blueberry and snow lotus herb, preparation method and application thereof - Google Patents
Fermented extract of blueberry and snow lotus herb, preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
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Abstract
The invention discloses a fermentation extract of blueberries and snow lotus herb, a preparation method and application thereof. The blueberry and snow lotus herb fermented extracts are fermented extracts obtained by fermenting the blueberry and the snow lotus herb together by using saccharomyces cerevisiae, and the blueberry and the snow lotus herb are fermented by the saccharomyces cerevisiae to obtain products comprising three extracts including the blueberry extract, the snow lotus herb extract and the saccharomyces cerevisiae fermented extract. The combination of the three extracts has the effects of synergy, moisture retention and oxidation resistance, and has wide application prospect in cosmetics for helping skin to resist dryness and damage.
Description
Technical Field
The invention relates to the field of natural medicinal chemistry and the technical field of cosmetics, and particularly relates to a fermentation extract of blueberries and snow lotus herb, a preparation method and application thereof.
Background
The skin is composed of epidermis, dermis and subcutaneous adipose tissue, each layer containing moisture. The amount of water content in the skin is the key to whether the skin is young, tender, plump, glossy and soft. When the moisture content of the skin of the infant is high, the skin of the old appears delicate and glossy, and the skin of the old appears dry, withered, desquamated and even wrinkled due to low moisture content.
The moisturizing is a permanent subject, is an important condition for ensuring the health of skin and delaying aging, and is a target for people to develop and research moisturizers with excellent performance.
On the one hand, with age, the skin moisturizing mechanism is damaged, the water content in skin tissue cells and among cells is reduced, and when the water content of the stratum corneum is reduced to below 10%, the skin becomes dry, loses elasticity, wrinkles and accelerates skin aging. On the other hand, the skin is affected by heat, cold, wind, detergent, ultraviolet radiation, etc., and the top layer becomes drier. Therefore, it is important to maintain moisture in the skin and reduce moisture loss. Currently, skin moisturizers on the market mainly comprise a sealing agent, a moisture absorbent, a hydrophilic matrix, a light-proofing agent and some special additive ingredients. The development of novel skin care products which do not contain preservatives, can meet the requirements of different consumers and have better skin care effect is urgently needed.
In view of this, the present invention is proposed.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provide a fermentation extract of blueberries and snow lotus herb, a preparation method and application thereof.
The embodiment of the invention is realized by the following steps:
a fermentation extract of blueberry and snow lotus herb is obtained by fermenting blueberry and snow lotus herb by using yeast, and the fermentation extract comprises the blueberry extract, the snow lotus herb extract and the yeast fermentation extract.
A method for preparing fermented extracts of blueberry and snow lotus herb comprises the following steps: fermenting fructus Myrtilli and herba Saussureae Involueratae with yeast.
A cosmetic for non-therapeutic purposes for aiding skin in resisting dry skin damage comprises fermented extracts of the blueberry and the snow lotus herb.
The invention has the following beneficial effects:
the invention discloses a fermentation extract of blueberries and snow lotus herb, a preparation method and application thereof. The blueberry and snow lotus herb fermented extracts are prepared by yeast fermentation, and are fermented by yeast, protein is decomposed in the fermentation process, so that the allergy probability is reduced, the fermented fragrance is increased, the blueberry and snow lotus herb are fermented and extracted together, the obtained fermented product contains the blueberry extract, the snow lotus herb extract and the yeast fermented extract, and the blueberry extract, the snow lotus herb extract and the yeast fermented extract are cooperated with one another, so that the blueberry and snow lotus herb fermented extract has the effects of synergism, moisture preservation and antioxidation, and can be widely applied to cosmetics helping skin to resist dry damage.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
FIG. 1 is a graph showing the radical DPPH clearance of extracts for different processes.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The traditional extraction process adopts water or organic solvent extraction, the recovery rate of the water extraction is low, and the organic solvent extraction has the consideration of the aspects of environment and safety. The saccharomyces cerevisiae is widely applied to brewing and food industries, has high safety, and has the advantages that fermentation metabolites of the saccharomyces cerevisiae contain rich B vitamins, amino acids, mineral substances, multiple vitamins, active substances and the like. In the fermentation process, the protein is decomposed, so that the probability of allergy is reduced, the fragrance of fermentation is increased, and the protein is more easily accepted by consumers after being added and used. The blueberry and the snow lotus herb are fermented and extracted together, and the obtained extracts have the synergistic effect. Can effectively extract and reduce the use of antiseptic.
In order to achieve the above purpose, the embodiment of the present invention adopts the following technical solutions:
in a first aspect, the embodiment of the invention provides a blueberry and snow lotus herb fermented extract, the blueberry and snow lotus herb fermented extract is obtained by fermenting blueberry and snow lotus herb with yeast, and the fermented extract comprises the blueberry extract, the snow lotus herb extract and the yeast fermented extract.
Blueberries are small berries of the genera Ericaceae (Ericaceae), Vaccinium (Vaccinium spp.) and are produced in america, Europe, Korea, Japan, and China. It is rich in polyphenol, flavone, anthocyanin, vitamin C, and other natural antioxidants. Has the following effects: protecting cells, preventing from being damaged by peroxide, preventing skin wrinkle from generating in advance, reducing dermatosis and waviness, eliminating scar, removing mottle, whitening skin, and making skin smooth and elastic.
Snow lotus is a plant of saussurea of Compositae, contains various chemical components such as flavonoid, alkaloid, polysaccharide, volatile alcohol and the like, and has various effects of resisting inflammation, resisting tumor, resisting aging, eliminating free radicals and the like.
The saccharomyces cerevisiae is widely applied to brewing and food industries, has high safety, and has the advantages that fermentation metabolites of the saccharomyces cerevisiae contain rich B vitamins, amino acids, mineral substances, multiple vitamins, active substances and the like.
The embodiment of the invention provides a blueberry and snow lotus herb fermented extract, the blueberry and snow lotus herb fermented extract is obtained by fermenting yeast, the yeast is used for fermentation, in the fermentation process, protein can be decomposed, the allergy probability is reduced, the fermented fragrance is increased, the blueberry and snow lotus herb are fermented and extracted together, the obtained product contains the blueberry extract, the snow lotus herb extract and the saccharomyces cerevisiae fermented extract, and the blueberry extract, the snow lotus herb extract and the saccharomyces cerevisiae fermented extract are cooperated to achieve the effects of synergism, moisture preservation and antioxidation.
In an alternative embodiment, the yeast used for fermentation is Saccharomyces cerevisiae; and the mass ratio of the blueberries to the snow lotus herb is 1-50: 1-50.
In a second aspect, an embodiment of the present invention provides a preparation method of the above fermented extracts of blueberry and snow lotus herb, including: fermenting fructus Myrtilli and herba Saussureae Involueratae with yeast. The blueberry and the snow lotus herb contain various components beneficial to skin, compared with the snow lotus herb, the blueberry and the snow lotus herb have more sugar and moisture, and after the blueberry and the snow lotus herb are mixed in a certain proportion, the material cost is saved compared with that of single fermentation, and more effective components are contained in a fermentation product, so that the blueberry and the snow lotus herb are beneficial to application.
In an alternative embodiment, the method comprises the following steps: and inoculating the prepared yeast seed liquid into the mixed liquid of the blueberry and the snow lotus herb for fermentation culture.
In an optional embodiment, the preparation of the mixed solution of the blueberry and the snow lotus herb comprises the following steps: mixing pulverized fructus Myrtilli and herba Saussureae Involueratae with water, and adjusting pH;
preferably, 1-50 parts of blueberry and 1-50 parts of snow lotus herb are crushed to 20-100 meshes, 1-50 parts of water is added, the mixture is uniformly mixed, the pH value is adjusted to 3-6, and pasteurization is carried out. The pH value of the mixed liquid after the blueberry and the snow lotus herb are uniformly mixed is adjusted to be 3-6, and when the pH value is the subsequent seed inoculation liquid, a proper growth environment is provided for the saccharomyces cerevisiae, the growth of the saccharomyces cerevisiae is promoted, the good state of the saccharomyces cerevisiae is ensured, and the fermentation is facilitated.
In an alternative embodiment, the preparation of the yeast seed liquid comprises the steps of: inoculating saccharomyces cerevisiae into a culture medium for culture;
preferably, the culture medium is inoculated with saccharomyces cerevisiae after being sterilized at the temperature of 115-121 ℃ for 15-20 minutes, and cultured for 6-24 hours at the temperature of 10-40 ℃;
more preferably, the composition of the medium is as follows: 1-2.5% of yeast powder, 2-3.5% of peptone and 2-4% of glucose. The sterilization operation is carried out on the saccharomyces cerevisiae culture medium, so that other mixed bacteria in the culture medium can be killed, and the growth of the saccharomyces cerevisiae is facilitated.
In an alternative embodiment, the fermentation culture comprises the steps of: inoculating the prepared yeast seed solution into the mixed solution, and culturing at 5-35 deg.C for 1-30 days, preferably at 20-25 deg.C for 7-10 days.
In an alternative embodiment, the method further comprises: centrifuging and filtering the fermentation liquor after the fermentation culture is finished, and collecting the product;
preferably, the fermentation liquor is heated to 50-70 ℃, kept warm for 2-24 hours, then centrifuged, and the centrifuged supernatant is filtered by a 0.22 mu m filter membrane to obtain the fermentation extracts of the blueberries and the snow lotus herb. The fermentation liquor is heated firstly and then centrifuged, because the further growth of the saccharomyces cerevisiae can be promoted by heat preservation again, so that the components in the blueberry and the snow lotus herb are released deeply, and the content of the extract in the fermentation product is improved.
In an alternative embodiment, the preparation of the fermented extracts of blueberry and snow lotus herb comprises the following specific steps:
preparing a mixed solution: crushing 1-50 parts of blueberry and 1-50 parts of snow lotus herb into 20-100 meshes, adding 1-50 parts of water, uniformly mixing, adjusting the pH value to 3-6, and performing pasteurization treatment;
preparing yeast seed liquid: preparing a culture medium: 1-2.5% of yeast powder, 2-3.5% of peptone and 2-4% of glucose, sterilizing the culture medium at the temperature of 115 ℃ for 15-20 minutes, inoculating saccharomyces cerevisiae, and culturing for 6-24 hours at the temperature of 10-40 ℃;
fermentation culture: adding the prepared yeast seed liquid into the mixed liquid, and culturing for 1-30 days at 5-35 ℃;
and (3) harvesting a product: heating the fermentation broth after fermentation culture to 50-70 deg.C, maintaining the temperature for 2-24 hr, centrifuging, and filtering the centrifuged supernatant with 0.22 μm filter membrane to obtain fermented extract of fructus Myrtilli and herba Saussureae Involueratae.
In a third aspect, the embodiment of the invention provides a cosmetic for assisting skin in resisting dry injury without treatment purpose, and the cosmetic comprises the fermented extracts of the blueberry and the snow lotus herb.
In the embodiment of the invention, the blueberry and the snow lotus herb are fermented and extracted together, and the obtained product contains the blueberry extract, the snow lotus herb extract and the saccharomyces cerevisiae fermented extract which are cooperated with each other, so that the blueberry and snow lotus herb fermented extract has synergistic moisturizing and antioxidant effects. Thus, the fermentation products described above can be used in cosmetics that help the skin against dry damage for non-therapeutic purposes.
The features and properties of the present invention are described in further detail below with reference to examples.
Example 1
The preparation method of the fermented extract of the blueberries and the snow lotus herb comprises the following steps:
inoculating yeast strain into seed culture medium, and culturing at 30 deg.C for 18 hr to obtain seed liquid 50 ml;
crushing 500g of frozen blueberries and 100g of snow lotus herb, sieving the crushed materials with a 50-mesh sieve, adding 1000g of purified water, uniformly stirring, adjusting the pH value to be 5.8, and performing pasteurization treatment to obtain a mixed solution;
inoculating the seed solution into the mixed solution, culturing at 25 deg.C for 20 days to obtain fermentation liquid of blueberry and herba Saussureae Involueratae, heating the fermentation liquid at 60 deg.C, maintaining the temperature for 4 hr, centrifuging, collecting supernatant, and filtering with 0.22 micrometer filter membrane to obtain fermented extract of blueberry and herba Saussureae Involueratae.
Example 2
The preparation method of the fermented extract of the blueberries and the snow lotus herb comprises the following steps:
inoculating yeast strain into seed culture medium, and culturing at 35 deg.C for 20 hr to obtain 50ml seed solution;
crushing 500g of frozen blueberries and 100g of snow lotus herb, sieving the crushed materials with a 50-mesh sieve, adding 1000g of purified water, uniformly stirring, adjusting the pH value to 6.0, and performing pasteurization treatment to obtain a mixed solution;
inoculating the seed solution into the mixed solution, culturing at 25 deg.C for 20 days to obtain fermentation liquid of blueberry and herba Saussureae Involueratae, heating the fermentation liquid to 65 deg.C, maintaining the temperature for 12 hr, centrifuging, collecting supernatant, and filtering with 0.22 micrometer filter membrane to obtain fermented extract of blueberry and herba Saussureae Involueratae.
Comparative example 1
The preparation method of the blueberry fermented extract comprises the following steps:
inoculating yeast strain into seed culture medium, and culturing at 30 deg.C for 18 hr to obtain seed liquid 50 ml;
crushing 500g of frozen blueberries, sieving the crushed blueberries with a 50-mesh sieve, adding 1000g of purified water, uniformly stirring, adjusting the pH value to 5.8, and performing pasteurization treatment to obtain a mixed solution;
inoculating the seed solution into the mixed solution, culturing at 25 deg.C for 20 days to obtain blueberry fermentation liquid, heating the fermentation liquid to 60 deg.C, maintaining the temperature for 4 hr, centrifuging, collecting supernatant, and filtering with 0.22 micrometer filter membrane to obtain blueberry fermentation extract.
Comparative example 2
The preparation method of the snow lotus herb fermented extract comprises the following steps:
inoculating yeast strain into seed culture medium, and culturing at 30 deg.C for 18 hr to obtain seed liquid 50 ml;
crushing 100g of snow lotus herb, sieving the crushed snow lotus herb with a 50-mesh sieve, adding 1000g of purified water and 50g of cane sugar, uniformly stirring, adjusting the pH value to 5.8, and performing pasteurization treatment to obtain a mixed solution;
inoculating the seed solution into the mixed solution, culturing at 25 deg.C for 20 days to obtain herba Saussureae Involueratae fermented solution, heating the fermented solution at 60 deg.C, maintaining the temperature for 4 hr, centrifuging, collecting supernatant, and filtering with 0.22 micrometer filter membrane to obtain herba Saussureae Involueratae fermented extract.
Comparative example 3
The preparation method of the blueberry ethanol extract comprises the following steps:
extraction processes in the references: (Zhao Huiyuan, Wu wenlong, Mali, Tengjihui, Yao Bei, Li Weilin blueberry polyphenol extraction process based on antioxidant activity analysis [ J ] food industry science and technology, 2015, 5 th stage).
Lyophilizing fresh herba Rubi Parvifolii, weighing 25g, respectively extracting with 70% ethanol containing 0.3% trifluoroacetic acid at a ratio of 1: 5(g/mL) at 30 deg.C under ultrasonic assisted extraction (power of 180W) for 3 times, each time for 30min, and halving the ratio of the 2 nd and 3 rd materials. Mixing extractive solutions, centrifuging at 4500r/min for 5min, collecting supernatant, concentrating under reduced pressure at 40-60 deg.C to remove solvent, and freeze drying the extract to obtain blueberry ethanol extract.
Comparative example 4
The preparation of the ethanol extract of the snow lotus herb comprises the following steps:
the extraction process in the reference (mengxiang, sun yong, zhangkai, li huoyun, huxing, preparation and detection method of saussurea involucrata extract research [ J ] daily chemical science, 2012, 12 th).
Making dried herba Saussureae Involueratae into powder, sieving with 80 mesh sieve, and placing herba Saussureae Involueratae powder into round-bottom flask. Adding 15mL of 70% ethanol solution into each gram of herba Saussureae Involueratae powder, heating and soaking at 80 deg.C for 2 times, each time for 1 hr, and mixing the 2 extractive solutions to obtain crude extractive solution. Concentrating the crude extract of herba Saussureae Involueratae under reduced pressure, and recovering ethanol to obtain suspension. The suspension was allowed to stand at low temperature (4 ℃) for 16h, centrifuged (3000r/m in) for 15min, and the supernatant was filtered off with celite and a Buchner funnel. Slowly adding isovolumetric butanediol into the water solution of the snow lotus herb extract obtained after centrifugation and filtration, stirring simultaneously, fully stirring uniformly, adding 1% active carbon, stirring for 30min, and performing suction filtration by using a sand core funnel to obtain a brown clear transparent liquid.
Evaluation of antioxidant activity and moisturizing effect:
the fermented products provided in the embodiment 1 and the comparative examples 1 to 4 of the invention, namely the blueberry, the snow lotus herb fermented extract, the blueberry ethanol extract, the snow lotus herb ethanol extract, the blueberry fermented extract and the snow lotus herb fermented extract, are subjected to DPPH removal experiments and skin moisture content experiments after being added into the facial mask for use according to the same raw material proportion, so that the antioxidant activity and the moisturizing effect are evaluated.
(1) And the antioxidant experiment detection of the extract:
DPPH free radical is a macromolecule with good stability by taking nitrogen as a center, the fading degree of an ethanol solution is in a linear relation with the number of electrons received by the ethanol solution, an absorption peak at the wavelength of 517nm is weakened after the DPPH free radical reacts with a substance with antioxidant activity, the antioxidant capacity is evaluated according to the change, the larger the DPPH clearance rate is, the stronger the antioxidant capacity is, and the method is an effective method commonly used for evaluating the antioxidant effect.
Dissolving 5mg DPPH in 100ml 95% ethanol;
1ml of sample was mixed (T) with 1ml of a solution of 95% ethanol and 2ml of ethanol DPPH;
1ml of sample was mixed with 3ml of 95% ethanol (T)0)；
2ml of ethanol DPPH solution mixed with 2ml of 95% ethanol (C);
4ml of 95% ethanol (C)0)；
After mixing, detecting the mixture after being protected from light for 30 minutes at the room temperature of 25 ℃;
three measurements per sample were averaged.
The radical DPPH clearance of the extract for the different processes described above is shown in fig. 1, where:
a is the DPPH clearance rate of free radicals of the fermented extracts of blueberry and snow lotus herb in the embodiment 1 of the invention;
b + C is the DPPH clearance of free radicals of the blueberry ethanol solution extract in the comparative example 3 and the snow lotus herb ethanol solution extract in the comparative example 4;
d + E is the radical DPPH clearance of the blueberry fermented extract of comparative example 1 + the snow lotus herb fermented extract of comparative example 2.
As can be seen from fig. 1: compared with DPPH free radical clearance, the separately fermented extracts are not as good as the products fermented together after being mixed in equal proportion, and the products extracted by ethanol are better than the products fermented respectively, so that the two products fermented together have obvious synergistic action.
(2) And moisture retention effect evaluation experiment:
adding 5 percent (a) of blueberry snow and lotus flower fermentation products into mask liquid, extracting a mixture (b) of blueberry and snow lotus flower by using an ethanol solution, performing blank comparison (d) of snow lotus flower fermentation products and blueberry fermentation products and mask liquid without extracts.
The population of 18-36 years of age was selected and randomly grouped into 20 individuals each. After the face is cleaned, the mask is applied for 20 minutes, the rest of the mask liquid is wiped dry for 3 minutes, the water content of the Skin is measured by an Antsci Skin-SP efficacy Skin tester and is calibrated to be 100%, and the water content of the Skin is measured again after 8 hours, and the test results are shown in Table 1.
Table 1 skin moisture test results
| Group of | a | b | c | d |
| Skin moisture content (%) | 81-93 | 53-68 | 62-70 | 51-64 |
As can be seen from table 1: the moisture content (%) of the skin of people who use the facial mask added with the fermentation product is 81-93, and compared with a blank group, the facial mask can effectively slow down the loss of skin moisture and has a moisturizing effect. The process is superior to ethanol solution extraction and the respective fermentation effects of the two.
In summary, the embodiments of the present invention provide a fermented extract of blueberry and snow lotus herb, a preparation method and an application thereof. The fermentation extracts of the blueberries and the snow lotus herb are obtained by fermenting the blueberries and the snow lotus herb together by using saccharomyces cerevisiae, and comprise the blueberry extracts, the snow lotus herb extracts and the saccharomyces cerevisiae fermentation extracts. Because the blueberries and the snow lotus herb contain various components beneficial to skin, compared with the snow lotus herb, the blueberries and the snow lotus herb have more sugar and moisture, and after the blueberries and the snow lotus herb are mixed according to a certain proportion, the material cost is saved compared with that of single fermentation. And the antioxidant activity and the moisturizing effect are better than those of single fermentation, which may be caused by the reasons that loss is reduced by co-fermentation and the two are matched to cause difference in yeast growth and metabolism and the like.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A fermentation extract of blueberries and snow lotus herb is characterized in that the fermentation extract of the blueberries and the snow lotus herb is obtained by fermenting the blueberries and the snow lotus herb by using yeast, and the fermentation extract comprises the blueberry extract, the snow lotus herb extract and the yeast fermentation extract.
2. The fermented extracts of blueberry and snow lotus herb as claimed in claim 1, wherein the yeast used for fermentation is saccharomyces cerevisiae; the mass ratio of the blueberries to the snow lotus herb is 1-50: 1-50.
3. A method for preparing fermented extracts of blueberry and snow lotus herb as claimed in claim 1 or 2, comprising: fermenting the blueberry and the snow lotus herb together by yeast.
4. The method for preparing fermented extracts of blueberry and snow lotus herb as claimed in claim 3, comprising the following steps: and inoculating the prepared yeast seed liquid into the mixed liquid of the blueberry and the snow lotus herb for fermentation culture.
5. The method for preparing fermented extracts of blueberry and snow lotus herb as claimed in claim 4, wherein the preparation of the mixed solution of blueberry and snow lotus herb comprises the following steps: mixing pulverized fructus Myrtilli and herba Saussureae Involueratae with water, and adjusting pH;
preferably, 1-50 parts of blueberry and 1-50 parts of snow lotus herb are crushed to 20-100 meshes, 1-50 parts of water is added, the mixture is uniformly mixed, the pH value is adjusted to 3-6, and pasteurization is carried out.
6. The method for preparing fermented extracts of blueberry and snow lotus herb as claimed in claim 4, wherein the preparation of the yeast seed liquid comprises the following steps: inoculating saccharomyces cerevisiae into a culture medium for culture;
preferably, the culture medium is inoculated with saccharomyces cerevisiae after being sterilized at the temperature of 115-121 ℃ for 15-20 minutes, and cultured for 6-24 hours at the temperature of 10-40 ℃;
more preferably, the composition of the medium is as follows: 1-2.5% of yeast powder, 2-3.5% of peptone and 2-4% of glucose.
7. The method for preparing fermented extracts of blueberry and snow lotus herb as claimed in claim 4, wherein the fermentation culture comprises the following steps: inoculating the prepared yeast seed solution into the mixed solution, and culturing at 5-35 deg.C for 1-30 days, preferably at 20-25 deg.C for 7-10 days.
8. The method for preparing fermented extracts of blueberry and snow lotus herb as claimed in claim 4, further comprising: centrifuging and filtering the fermentation liquor after the fermentation culture is finished, and collecting the product;
preferably, the fermentation liquor is heated to 50-70 ℃, kept warm for 2-24 hours, then centrifuged, and the centrifuged supernatant is filtered by a 0.1-0.22 μm filter membrane to obtain the fermentation extract of blueberry and snow lotus herb.
9. The method for preparing fermented extracts of blueberry and snow lotus herb as claimed in any one of claims 3-8, comprising the steps of:
preparing a mixed solution: crushing 1-50 parts of blueberry and 1-50 parts of snow lotus herb into 20-100 meshes, adding 1-50 parts of water, uniformly mixing, adjusting the pH value to 3-6, and performing pasteurization treatment;
preparing yeast seed liquid: preparing a culture medium: 1-2.5% of yeast powder, 2-3.5% of peptone and 2-4% of glucose, sterilizing the culture medium at the temperature of 115 ℃ for 15-20 minutes, inoculating saccharomyces cerevisiae, and culturing for 6-24 hours at the temperature of 10-40 ℃;
fermentation culture: adding the prepared yeast seed liquid into the mixed liquid, and culturing for 1-30 days at 5-35 ℃;
and (3) harvesting a product: heating the fermentation liquid after fermentation culture to 50-70 deg.C, keeping the temperature for 2-24 hr, centrifuging, and filtering the centrifuged supernatant with 0.22 μm filter membrane to obtain the fermented extract of fructus Myrtilli and herba Adonidis.
10. A non-therapeutic cosmetic for helping skin resist dry damage, which is characterized by comprising the fermented extracts of blueberries and snow lotus herb as claimed in any one of claims 1 to 2 or the fermented extracts of blueberries and snow lotus herb prepared by the preparation method as claimed in any one of claims 3 to 9.
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