CN111728083A - A kind of bacteria-enzyme fusion fermentation culture capable of improving the intestinal function of livestock and poultry and preparation method thereof - Google Patents

Abstract

The invention provides a bacterial-enzyme fusion fermentation culture capable of improving the intestinal function of livestock and poultry and a preparation method thereof, belonging to the field of biological feed preparation. It is characterized in that different mass parts of soybean meal, corn, bran, rice husk powder and molasses are used as fermentation substrates, and the formula combination of compound probiotics and compound enzyme preparation is optimized, and it is obtained by the fusion fermentation method of bacteria-enzyme. The preparation method of the invention has the advantages of simple operation, easy realization and low cost, and the prepared fermentation culture is rich in beneficial bacteria and metabolites, and has the functions of promoting animal feeding, improving the production performance of livestock and poultry, and reducing diseases such as intestinal diarrhea.

Description

A kind of bacteria-enzyme fusion fermentation culture with improving the intestinal function of livestock and poultry and its preparation backup method

technical field

The invention relates to a bacteria-enzyme fusion fermentation culture capable of improving the intestinal function of livestock and poultry and a preparation method thereof, belonging to the field of biological feed preparation.

Background technique

Antibacterial growth promoters (AGPs) have obviously brought huge economic benefits to the aquaculture industry in terms of ensuring animal health, improving production performance, and reducing production costs. However, serious problems such as drug resistance of pathogenic bacteria, drug residues, and environmental pollution caused by long-term use of AGPs have become increasingly prominent, which have endangered human health and safety. Therefore, the prohibition of antibiotics in feed has become inevitable. my country has clearly stipulated that the use of antibiotic growth promoters in feed will be prohibited from July 1, 2020. However, due to the low intestinal health of animals brought about by the ban on feeding antibiotics, the problems of reduced production performance and increased mortality will become increasingly prominent. Therefore, how to effectively solve the breeding problem caused by antibiotics is a scientific research task that is both arduous and must be solved.

Gut health determines the overall function and productivity of an animal, and ensuring gut health is the number one priority in farming. Bio-fermentation feed or culture is obtained by treating raw materials with microorganisms, which can not only remove some antigenic substances in the raw materials, but also degrade some macromolecular substances such as polysaccharides, proteins, and fats through the metabolism of microorganisms to generate organic acids, Small molecular substances such as soluble polypeptides play an important role in animal growth and development. More importantly, the probiotics in the fermented culture can create an anaerobic environment by consuming the oxygen in the intestinal tract after entering the animal intestine, thereby helping to maintain the intestinal ecological balance; and probiotics can produce antagonizing intestinal pathogenicity. A variety of bacteria to promote intestinal health.

At present, there is no unified standard for bio-fermented feed, and the products on the market are relatively chaotic, mostly based on a single raw material fermented by microorganisms, such as fermented soybean meal. The purpose of fermentation is to eliminate antigens and improve feed utilization. In the fermentation technology, after the accumulation of solids is fermented, the drying treatment is the main method. This method has high cost and low probiotic activity, which does not meet the actual needs of the farm. Therefore, it is urgent to develop and explore fermentation methods for different fermentation substrates to improve the generation rate of metabolites to adapt to current farming.

SUMMARY OF THE INVENTION

Based on the above problems, the present invention obtains high-performance microbial fermentation culture products by screening different fermentation substrate combinations, designing probiotics and compound enzyme formulas, and using advanced bacteria-enzyme fusion fermentation technology and suitable fermentation conditions.

In a first aspect, the present invention provides a bacterial-enzyme fusion fermentation culture with improved intestinal function of livestock and poultry.

The fusion fermentation culture is composed of the following components in parts by weight: 50-150 parts of soybean meal, 60-240 parts of corn, 120-380 parts of bran, 130-400 parts of rice husk powder, 30-130 parts of molasses, compound 1 to 4 parts of probiotic powder, 1 to 5 parts of compound enzyme powder, and 270 to 340 parts of water.

Preferably, 60-120 parts of soybean meal, 80-200 parts of corn, 140-300 parts of bran, 150-340 parts of rice husk powder, 60-110 parts of molasses, 1-3 parts of compound probiotic powder, 2-3 parts of compound enzyme powder 4 servings, 280-320 servings of water.

In a specific embodiment, the composite probiotic powder is composed of Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus acidophilus, and Enterococcus faecium, wherein 0.2 to 1 part of Saccharomyces cerevisiae, 0.1 part of Bacillus subtilis ～0.8 part, Bacillus licheniformis 0.1～0.8 part, Lactobacillus plantarum 0.1～0.8 part, Lactobacillus acidophilus 0.1～0.8 part, Enterococcus faecium 0.1～0.8 part.

In a specific embodiment, the compound enzyme powder is composed of xylanase, β-glucanase, cellulase, acid protease, neutral protease, and pectinase, wherein 0.1-0.8 parts of xylanase, β-glucanase 0.2-1.2 parts of glucanase, 0.1-1 part of cellulase, 0.1-1 part of acid protease, 0.1-1 part of neutral protease, and 0.1-0.8 part of pectinase.

In a specific embodiment, the total viable count of the composite probiotic powder is 2.0×10 ⁸ to 5.0×10 ¹¹ cfu/g. The viable counts of Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus acidophilus, and Enterococcus faecium were all 1.0×10 ⁶ to 3.0×10 ¹⁰ cfu/g.

In a specific embodiment, in the composite enzyme powder, xylanase 3000-24000U/g, β-glucanase 1000-6000U/g, cellulase 200-2000U/g, acid protease 3000-30000U/g, Sex protease 5000～50000U/g, pectinase 3000～240000000U/g.

In a second aspect, the present invention provides a method for preparing the fermented culture in the first aspect, comprising the following steps:

1) Passing soybean meal, corn, bran and rice husk powder through an analytical sieve, and the mesh number of the sieve holes is 20-40 meshes;

2) Weigh the composite probiotic powder in proportion and dissolve it with 10 to 20 times of water, and activate it for later use;

3) Weigh the compound enzyme powder in proportion and dissolve it with 10 to 20 times of water, and activate it for later use;

4) take by weighing and dilute the molasses with the remaining water for subsequent use;

5) The fermentation mixing equipment and site are cleaned and disinfected;

6) Weigh soybean meal, corn, bran, and rice husk powder in proportion and place them in a mixer, and mix for 3 to 8 minutes;

7) spray the molasses diluted in step 4) into the mixer of step 6), and continue to mix for 3 to 10 minutes;

8) spray the compound probiotic liquid in step 2) and the compound enzyme liquid in step 3) into the mixer of step 6), and continue to mix for 5-10 minutes;

9) The uniformly mixed mixture in the mixer in step 6) is put into a fermentation bag, placed in a fermentation room at 28-34°C, and fermented for 72-120 hours to obtain the culture.

The above-mentioned fermentation bag adopts a new type of fermentation feed bag, a patented product (Patent Publication No. CN207903051U), which utilizes heat-sealing technology and is directly provided with at least one heat-sealing structure capable of exhausting air on the bag body. The fermentation bag is not only simple to manufacture and low in cost, but also can greatly reduce the risk of mold contamination near the exhaust valve and the phenomenon of bag swelling, and improve the success rate of fermentation.

Preferably, the water temperature for the activation of the above-mentioned composite probiotics is 28-35° C., and the activation time is 1.5-2.5 hours.

Preferably, the water temperature for the above-mentioned composite enzyme activation is 32-42°C, and the activation time is 1-2 hours

In a third aspect, the present invention provides the application of the fermentation culture described in the first aspect and the fermentation culture prepared in the second aspect in livestock and poultry. The application can be used in any stage of livestock and poultry, and can be embodied in any one of the following 1) and 2):

1) Application in increasing the daily feed intake and/or daily gain of livestock and poultry;

2) Application in reducing intestinal diseases such as diarrhea in livestock and poultry.

Beneficial effects of the present invention:

1) The matching of the fermentation substrate raw material and the bacterial enzyme selection of the present invention is excellent: in order to save costs, the local raw material resources are used to prepare the fermentation substrate. Through the analysis of nutrients and antigens based on the formula of fermentation substrate raw materials, combined with the characteristics of animal intestinal physiology, compound enzymes and compound bacteria are rationally prepared. in order to achieve the best fermentation effect.

2) Application of bacterial-enzyme fusion fermentation technology in the present invention: bacterial-enzyme fusion fermentation process, bacterial metabolism and heat production are conducive to enzymatic hydrolysis reaction, enzymatic hydrolysis product can provide nutrition for bacterial reproduction, and synergistic increase between enzymatic reaction and fermentation reaction. It is beneficial to produce more active metabolites and improve the actual use effect.

3) The present invention adopts novel fermented feed bag for fermentation: the culture obtained by fermentation in the fermented bag can completely retain the activity of a large number of beneficial live bacteria and metabolites because it does not undergo a drying process, thereby improving the effect of intestinal health care. Compared with the one-way valve fermentation bag, the new type of fermentation feed bag can greatly reduce the risk of mold contamination near the exhaust valve and the phenomenon of bag breaking, and improve the success rate of fermentation.

4) The preparation of the present invention is simple and practical: the preparation process of the product of the present invention is simple, the investment is low, and the return is high, which is very suitable for the needs of breeding farms.

5) The product test results are outstanding: After testing, this product is rich in probiotics, lactic acid, oligosaccharides, enzymes, functional small peptides, and unknown growth factors and other biologically active substances. The products have outstanding effects in improving animal feed intake, promoting animal growth, and reducing the incidence of intestinal diseases.

Description of drawings

Figure 1: Flow chart of the preparation method of bacteria-enzyme fusion fermentation culture for improving the intestinal function of livestock and poultry.

Detailed ways

The present invention will be further described below in conjunction with specific embodiments, but the present invention is not limited to the following embodiments. The methods are conventional methods unless otherwise specified. The raw materials can be obtained from open commercial sources unless otherwise specified.

Example 1 Bacteria-enzyme fusion fermentation culture for improving the intestinal function of livestock and poultry

A bacterial-enzyme fusion fermentation culture capable of improving the intestinal function of livestock and poultry is composed of the following components by weight: 80 parts of soybean meal, 125 parts of corn, 175 parts of bran, 245 parts of rice husk powder, and 70 parts of molasses , 2 parts of compound probiotic powder, 2.5 parts of compound enzyme powder, 300.5 parts of water. The compound probiotic powder is composed of the following components in parts by weight: 0.4 part of Saccharomyces cerevisiae, 0.3 part of Bacillus subtilis, 0.3 part of Bacillus licheniformis, 0.4 part of Lactobacillus plantarum, 0.3 part of Lactobacillus acidophilus, feces intestine 0.3 copies of cocci. The compound enzyme powder is composed of the following components in parts by weight: 0.4 part of xylanase, 0.6 part of beta-glucanase, 0.4 part of cellulase, 0.4 part of acid protease, 0.4 part of neutral protease, Glyase 0.3 parts.

The viable counts of Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus acidophilus, and Enterococcus faecium are all 1.0×10 ⁷ to 3.0×10 ⁹ cfu/g.

The xylanase 10000～14000U/g, β-glucanase 2000～3500U/g, cellulase 300～600U/g, acid protease 9000～15000U/g, neutral protease 18000～25000U/g , pectinase 7000 ~ 12000U/g.

The above-mentioned preparation method of the bacteria-enzyme fusion fermentation culture provided by the present invention with improving the intestinal function of livestock and poultry is carried out according to the following steps:

1) Soybean meal, bran and rice husk powder are sieved, and the mesh number of the sieve is 30 meshes;

2) Weigh the composite probiotic powder in proportion and dissolve it with 15 times of water, and activate it for subsequent use;

3) Weigh the composite enzyme powder in proportion to dissolve it with 15 times of water, and activate it for subsequent use;

4) take by weighing and dilute the molasses with the remaining water for subsequent use;

5) The fermentation mixing equipment and site are cleaned and disinfected;

6) take soybean meal, bran, rice husk powder in proportion and place in mixer, mix for 5 minutes;

7) The molasses diluted in step 4) is sprayed into the mixer of step 6), and continues to mix for 5 minutes;

8) spray the compound probiotic liquid in step 2) and the compound enzyme liquid in step 3) into the mixer of step 6), and continue to mix for 8 minutes;

9) The uniformly mixed mixture in the mixer in step 6) is put into a fermentation bag, placed in a fermentation room at 32°C, and fermented for 120 hours to obtain the culture.

The water temperature for activation of the above-mentioned composite probiotics is 32° C., and the activation time is 2 hours.

The water temperature for the above-mentioned composite enzyme activation was 40° C., and the activation time was 2 hours.

In the above step 9), the fermentation bag adopts a new type of fermentation feed bag, a patented product (Patent Publication No. CN207903051U), which utilizes heat sealing technology to directly provide at least one ventable heat sealing structure on the bag body. The fermentation bag is not only simple to manufacture and low in cost, but also can greatly reduce the risk of mold contamination near the exhaust valve and the phenomenon of bag swelling, and improve the success rate of fermentation.

Example 2 The bacteria-enzyme fusion fermentation culture that improves the intestinal function of livestock and poultry

A bacterial-enzyme fusion fermentation culture capable of improving the intestinal function of livestock and poultry is composed of the following components by weight: 80 parts of soybean meal, 125 parts of corn, 175 parts of bran, 245 parts of rice husk powder, and 70 parts of molasses , 2.5 parts of compound probiotic powder, 2.5 parts of compound enzyme powder, 300 parts of water. The compound probiotic powder is composed of the following components by weight: 0.5 part of Saccharomyces cerevisiae, 0.4 part of Bacillus subtilis, 0.4 part of Bacillus licheniformis, 0.4 part of Lactobacillus plantarum, 0.4 part of Lactobacillus acidophilus, feces intestine 0.4 copies of cocci. The compound enzyme powder is composed of the following components in parts by weight: 0.4 part of xylanase, 0.6 part of beta-glucanase, 0.4 part of cellulase, 0.4 part of acid protease, 0.4 part of neutral protease, Glyase 0.3 parts.

The viable counts of Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus acidophilus, and Enterococcus faecium are all 1.0×10 ⁷ to 3.0×10 ⁹ cfu/g.

The xylanase 10000～14000U/g, β-glucanase 2000～3500U/g, cellulase 300～600U/g, acid protease 9000～15000U/g, neutral protease 18000～25000U/g , pectinase 7000 ~ 12000U/g.

The above-mentioned preparation method of the bacteria-enzyme fusion fermentation culture provided by the present invention with improving the intestinal function of livestock and poultry is carried out according to the following steps:

1) Soybean meal, bran and rice husk powder are sieved, and the mesh number of the sieve is 30 meshes;

2) Weigh the composite probiotic powder in proportion and dissolve it with 10 times of water, and activate it for subsequent use;

3) Weigh the composite enzyme powder in proportion and dissolve it with 10 times of water, and activate it for subsequent use;

4) take by weighing and dilute the molasses with the remaining water for subsequent use;

5) The fermentation mixing equipment and site are cleaned and disinfected;

6) take soybean meal, bran, rice husk powder in proportion and place in mixer, mix for 5 minutes;

7) The molasses diluted in step 4) is sprayed into the mixer of step 6), and continues to mix for 5 minutes;

8) spray the compound probiotic liquid in step 2) and the compound enzyme liquid in step 3) into the mixer of step 6), and continue to mix for 8 minutes;

9) The uniformly mixed mixture in the mixer in step 6) is put into a fermentation bag, placed in a fermentation room at 30° C., and fermented for 96 hours to obtain the culture.

The water temperature for activation of the above-mentioned composite probiotics is 30° C., and the activation time is 1.5 hours.

The water temperature for the above-mentioned composite enzyme activation was 35°C, and the activation time was 1 hour.

In the above step 9), the fermentation bag adopts a new type of fermentation feed bag, a patented product (Patent Publication No. CN207903051U), which utilizes heat sealing technology to directly provide at least one ventable heat sealing structure on the bag body. The fermentation bag is not only simple to manufacture and low in cost, but also can greatly reduce the risk of mold contamination near the exhaust valve and the phenomenon of bag swelling, and improve the success rate of fermentation.

Example 3 The bacteria-enzyme fusion fermentation culture that improves the intestinal function of livestock and poultry

A bacteria-enzyme fusion fermentation culture capable of improving the intestinal function of livestock and poultry is composed of the following components in parts by weight: 65 parts of soybean meal, 95 parts of corn, 148 parts of bran, 300 parts of rice husk powder, 90 parts of molasses, 2 parts of compound probiotic powder, 2 parts of compound enzyme powder, 298 parts of water.

The composite probiotic powder is composed of the following components by weight: 0.5 part of Saccharomyces cerevisiae, 0.3 part of Bacillus subtilis, 0.3 part of Bacillus licheniformis, 0.3 part of Lactobacillus plantarum, 0.3 part of Lactobacillus acidophilus, feces intestine 0.3 copies of cocci. The compound enzyme powder is composed of the following components by weight: 0.3 part of xylanase, 0.5 part of β-glucanase, 0.4 part of cellulase, 0.3 part of acid protease, 0.3 part of neutral protease, and 0.3 part of neutral protease. Gluease 0.2 part.

The viable counts of Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus acidophilus, and Enterococcus faecium are all 1.0×10 ⁷ to 3.0×10 ⁹ cfu/g.

The xylanase 7000～12000U/g, β-glucanase 2000～3000U/g, cellulase 600～1000U/g, acid protease 7000～12000U/g, neutral protease 12000～18000U/g , pectinase 4000 ~ 8000U/g.

The preparation method of the bacteria-enzyme fusion fermented culture provided by the present invention with improved intestinal function of livestock and poultry is the same as that in Example 1.

Example 4 Bacteria-enzyme fusion fermentation culture for improving the intestinal function of livestock and poultry

A bacteria-enzyme fusion fermentation culture capable of improving the intestinal function of livestock and poultry is composed of the following components by weight: 100 parts of soybean meal, 180 parts of corn, 170 parts of bran, 164 parts of rice husk powder, and 80 parts of molasses , 2.5 parts of compound probiotic powder, 3.0 parts of compound enzyme powder, 300.5 parts of water.

The compound probiotic powder is composed of the following components by weight: 0.5 part of Saccharomyces cerevisiae, 0.4 part of Bacillus subtilis, 0.4 part of Bacillus licheniformis, 0.4 part of Lactobacillus plantarum, 0.4 part of Lactobacillus acidophilus, feces intestine 0.4 copies of cocci. The compound enzyme powder is composed of the following components in parts by weight: 0.6 part of xylanase, 0.7 part of β-glucanase, 0.4 part of cellulase, 0.5 part of acid protease, 0.5 part of neutral protease, and 0.5 part of neutral protease. Glyase 0.3 parts.

The viable counts of Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus acidophilus, and Enterococcus faecium are all 1.0×10 ⁷ to 3.0×10 ⁹ cfu/g.

The xylanase 15000～20000U/g, β-glucanase 2500～4500U/g, cellulase 600～1000U/g, acid protease 12000～18000U/g, neutral protease 18000～30000U/g , pectinase 70-120 million U/g.

The preparation method of the bacteria-enzyme fusion fermented culture provided by the present invention with improved intestinal function of livestock and poultry is the same as that in Example 1.

Example 5 Evaluation of bacteria-enzyme fusion fermentation culture for improving the intestinal function of livestock and poultry

1. Observation of fermentation process

During the fermentation process, it was found that obvious gas production began to appear about 10 hours after the fermentation, and the fermentation room was filled with the aroma of fermentation. At the same time, the fermentation breathing bag had flatulence phenomenon, indicating that the fermentation gas production rate was faster than the exhaust valve exhaust process at this time, indicating that this When the fermentation gas production is large, the gas production speed is fast; about 30 hours, the flatulence phenomenon slowly disappears, indicating that the fermentation gas production begins to slow down at this time, and a balance is formed between the gas production and the exhaust valve exhaust. At the end of the fermentation, the samples were taken and observed that, compared with those before fermentation, the color of the samples after fermentation became darker, the texture was soft, and the sour and fragrant taste of fermentation was diffused at the same time.

2. Laboratory determination

For fermented products, we pay more attention to indicators such as acidity, probiotics and acid-soluble protein. It can be seen from the test that compared with before fermentation, the total acid content, the ratio of acid-soluble protein and the number of various viable bacteria of the product after fermentation are significantly increased, of which the proportion of total acid is improved by 236%, and the proportion of acid-soluble protein is increased by 86.48%. , the number of various viable bacteria increased by 2 orders of magnitude (the test results are shown in Table 1).

index Sample before fermentation post-fermentation samples Moisture, % 40.8 41.4 Total acid (calculated as lactic acid), % 0.91 3.06 pH 6.71 4.82 Crude protein, % 9.62 10.31 Acid soluble protein, % 2.81 5.24 Acid-soluble protein/crude protein, % 29.2 50.82 Bacillus (cfu/g) 2.3×10⁶ 4.2×10⁸ Lactic acid bacteria (cfu/g) 3.6×10⁶ 3.6×10⁸ Yeast (cfu/g) 2.1×10⁶ 2.2×10⁸

3. Animal test

1) Experiment on growing and finishing pigs

Experimental design: The experiment selected 120 ternary hybrid weaning and nursery piglets with healthy, normal growth and development and a similar weight of about 24.23 kg, and were randomly divided into 2 groups: the control group and the experimental group. The control group was fed the original basal diet (self-prepared compound feed of 4% commercial premix), and the test group was supplemented with 8% of the bacteria that can improve the intestinal function of livestock and poultry obtained in Example 4 of the present invention on the basis of the basal diet. - Enzyme fusion fermentation cultures. This experiment was conducted in a pig farm in Shanxi from October 10, 2019 to November 25, 2019. The experimental period was 42 days, and the pre-feeding period was 5 days.

Feeding and management: During the experimental period, the animals were operated according to the routine feeding and management procedures of the pig farm, with free access to food and water, and immunization according to the immunization procedure of the pig farm.

Determination of indicators: at the beginning and end of the experiment, the fasting weight was taken as the initial weight and the final weight of the experiment before feeding on the same day; the feed consumption was recorded in detail in the unit of repetition; the occurrence of diarrhea in each group was recorded; the average daily weight gain, feed weight ratio and diarrhea rate. Daily weight gain=(final weight-initial weight)/test days; feed-to-weight ratio=average daily feed intake/average daily gain; diarrhea rate=total diarrhea rate times/(total number of heads×test days)×100%) .

Test results: see the table below

control group test group Average initial weight, kg 24.32 24.14 Average final weight, kg 50.35 52.23 Average daily gain, g 619.76 668.81 Average daily feed intake, kg 1.65 1.71 meat to meat ratio 2.66 2.56 Diarrhea rate, % 4.34 2.75

Evaluation:

During the experiment, it was found that compared with the control group, the pigs in the experimental group had higher feeding motivation, higher uniformity of the pig herd; better feces formation, fewer intestinal disease problems, and significantly lower treatment costs.

The above results show that compared with the control group, the average daily gain of pigs in the experimental group increased by about 50g, and the feed-to-weight ratio decreased by about 0.1; the diarrhea rate decreased by 57.82%. It is indicated that the bacterial-enzyme fusion fermentation culture with improving the intestinal function of livestock and poultry obtained by the present invention has good effects in improving the production performance of growing and fattening pigs and preventing intestinal diseases.

2) Layer test

Experimental design: The experiment selected 5,000 350-day-old Hylan brown laying hens and randomly divided them into 2 groups: the control group and the experimental group. The control group was fed the original compound diet (it was a self-made compound feed of 5% commercial premix), and the test group was supplemented with 5% of the bacteria that can improve the intestinal function of livestock and poultry obtained in Example 4 of the present invention on the basis of the basal diet. - Enzyme fusion fermentation cultures. This experiment was conducted in a laying hen farm in Baodi, Tianjin from October 18, 2019 to December 13, 2019. The experimental period was 56 days.

Feeding and management: Each group of laying hens adopts a unified management system, unified light intensity, light time, good ventilation and suitable humidity, and routinely carries out preventive vaccination and preventive disinfection.

Determination of indicators: Accurately record the number of eggs laid, the number of broken eggs, the weight of laid eggs, the amount of material consumed, and the number of dead eggs in each group. Calculate egg production rate, breakage rate and feed-to-egg ratio.

Test results: see the table below

control group test group Experiments began to be stocked, only 2500 2500 Inventory at the end of the test, only 2478 2483 The number of dead scouring, only twenty two 17 total number of eggs laid 120236 121134 Number of broken eggs, pieces 1724 1548 Average egg production rate during the test period, % 86.14 86.65 Egg breakage rate, % 1.43 1.28 Total egg weight during the test period, kg 7558.75 7617.45 The total consumption of the test period, kg 17632 17655 egg ratio 2.33 2.32 Death rate, % 0.88 0.68

Evaluation:

During the test, it was observed that from the 7th day of the test, the feces and water samples of the experimental group of laying hens were significantly less than those of the control group. The above results show that, compared with the control group, the egg laying rate of the experimental group increased by 0.5%, and the broken egg rate and the dead panning rate were reduced to varying degrees. It is indicated that the bacteria-enzyme fusion fermentation culture with improving the intestinal function of livestock and poultry obtained by the present invention has good effects in improving the egg-laying performance of laying hens in the later stage of egg-laying and preventing intestinal diseases.

It should be noted that, according to the above-mentioned embodiments of the present invention, those skilled in the art can fully realize the full scope of the independent claims and dependent rights of the present invention, and the implementation process and method are the same as the above-mentioned embodiments; and the present invention is not elaborated. Part of it belongs to the well-known technology in the art.

The above description is only a part of the specific embodiments of the present invention, but the protection scope of the present invention is not limited to this. Any person familiar with the art within the technical scope disclosed by the present invention can easily think of changes or substitutions. Included within the scope of protection of the present invention.

Claims (9)

1. A bacteria-enzyme fusion fermentation culture for improving intestinal functions of livestock and poultry is characterized in that: the fusion fermentation culture comprises the following components in parts by weight: 50-150 parts of soybean meal, 60-240 parts of corn, 120-380 parts of bran, 130-400 parts of rice hull powder, 30-130 parts of molasses, 1-4 parts of composite probiotic powder, 1-5 parts of composite enzyme powder and 340 parts of water 270-containing materials;

preferably, the composite probiotic powder consists of saccharomyces cerevisiae, bacillus subtilis, bacillus licheniformis, lactobacillus plantarum, lactobacillus acidophilus and enterococcus faecium; the compound enzyme powder consists of xylanase, beta-glucanase, cellulase, acid protease, neutral protease and pectinase.

2. The fused fermentation culture of claim 1, wherein: the fusion fermentation culture comprises the following components in parts by weight: 60-120 parts of soybean meal, 80-200 parts of corn, 140-300 parts of bran, 150-340 parts of rice hull powder, 60-110 parts of molasses, 1-3 parts of composite probiotic powder, 2-4 parts of composite enzyme powder and 320 parts of water 280-doped materials;

further preferably, in the composite probiotic powder, 0.2-1 part of saccharomyces cerevisiae, 0.1-0.8 part of bacillus subtilis, 0.1-0.8 part of bacillus licheniformis, 0.1-0.8 part of lactobacillus plantarum, 0.1-0.8 part of lactobacillus acidophilus and 0.1-0.8 part of enterococcus faecium are added;

further preferably, in the compound enzyme powder, 0.1-0.8 part of xylanase, 0.2-1.2 parts of beta-glucanase, 0.1-1 part of cellulase, 0.1-1 part of acid protease, 0.1-1 part of neutral protease and 0.1-0.8 part of pectinase are included.

3. The fusion fermentation culture of claim 2, wherein the total viable count of the composite probiotic powder is 2.0 × 10⁸～5.0×10¹¹cfu/g, wherein the viable count of Saccharomyces cerevisiae, Bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, Lactobacillus acidophilus and enterococcus faecium is 1.0 × 10⁶～3.0×10¹⁰cfu/g。

4. The fused fermentation culture of claim 2, wherein: in the compound enzyme powder, 3000-24000U/g of xylanase, 1000-6000U/g of beta-glucanase, 200-2000U/g of cellulase, 3000-30000U/g of acid protease, 5000-50000U/g of neutral protease and 3000-24000 ten thousand U/g of pectinase are contained.

5. A process for the preparation of a confluent fermentation culture according to claims 1-4, characterized in that: the method comprises the following steps:

1) sieving the soybean meal, the corn, the bran and the rice hull powder by using an analysis sieve, wherein the mesh number of sieve pores is 20-40 meshes;

2) weighing the composite probiotic powder according to a proportion, dissolving the composite probiotic powder with 10-20 times of water, and activating for later use;

3) weighing the compound enzyme powder according to a proportion, dissolving the compound enzyme powder with 10-20 times of water, and activating for later use;

4) weighing molasses according to a proportion, and diluting the molasses with the rest water for later use;

5) cleaning and disinfecting fermentation mixing equipment and a field;

6) weighing the soybean meal, the corn, the bran and the rice hull powder according to a certain proportion, placing the mixture into a mixer, and mixing for 3-8 minutes;

7) spraying the diluted molasses in the step 4) into the mixer in the step 6), and continuously mixing for 3-10 minutes;

8) spraying the composite probiotic liquid in the step 2) and the composite enzyme liquid in the step 3) into the mixer in the step 6), and continuously mixing for 5-10 minutes;

9) and (3) putting the uniformly mixed mixture in the mixer in the step 6) into a fermentation bag, placing the fermentation bag into a fermentation room at the temperature of 28-34 ℃, and fermenting for 72-120 hours to obtain the culture.

6. The method of claim 5, wherein: the water temperature for activating the composite probiotics is 28-35 ℃, and the activation time is 1.5-2.5 hours.

7. The method of claim 5, wherein: the temperature of the activating water of the compound enzyme is 32-42 ℃, and the activating time is 1-2 hours.

8. Use of the fused fermentation culture according to any one of claims 1 to 4 and the fused fermentation culture obtained by the production method according to any one of claims 5 to 7 in livestock.

9. The use of claim 8, wherein: the application can be used in any stage of livestock and poultry, and can be specifically represented by any one of the following 1) and 2):

1) the application in improving daily feed intake and/or daily gain of livestock and poultry;

2) can be used for reducing intestinal diseases such as diarrhea in livestock and poultry.

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