CN111700905A - Chitosan and preparation method thereof - Google Patents
Chitosan and preparation method thereof Download PDFInfo
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- CN111700905A CN111700905A CN202010601621.0A CN202010601621A CN111700905A CN 111700905 A CN111700905 A CN 111700905A CN 202010601621 A CN202010601621 A CN 202010601621A CN 111700905 A CN111700905 A CN 111700905A
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- 229920001661 Chitosan Polymers 0.000 claims description 62
- 229960000892 attapulgite Drugs 0.000 claims description 59
- 229910052625 palygorskite Inorganic materials 0.000 claims description 59
- 239000000243 solution Substances 0.000 claims description 42
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 39
- 238000003756 stirring Methods 0.000 claims description 27
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 26
- 239000002131 composite material Substances 0.000 claims description 23
- 239000000419 plant extract Substances 0.000 claims description 23
- 238000002360 preparation method Methods 0.000 claims description 21
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 20
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 20
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 20
- 238000001035 drying Methods 0.000 claims description 20
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 20
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 239000008367 deionised water Substances 0.000 claims description 19
- 229910021641 deionized water Inorganic materials 0.000 claims description 19
- 239000003292 glue Substances 0.000 claims description 18
- 229940037003 alum Drugs 0.000 claims description 16
- 229920002678 cellulose Polymers 0.000 claims description 16
- 239000001913 cellulose Substances 0.000 claims description 16
- 150000003385 sodium Chemical class 0.000 claims description 15
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- 241000205585 Aquilegia canadensis Species 0.000 claims description 6
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- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
- 208000030453 Drug-Related Side Effects and Adverse reaction Diseases 0.000 description 1
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- 125000001664 diethylamino group Chemical group [H]C([H])([H])C([H])([H])N(*)C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
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- 239000000463 material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
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Abstract
The invention discloses chitosan and a preparation method thereof, belonging to the technical field of medical treatment, and the chitosan and the preparation method thereof mainly comprise chitosan gel, wherein the chitosan gel comprises the following components in percentage by mass: the chitosan gel comprises chitosan gel as a main component, wherein the chitosan gel comprises chitosan, lactic acid solution, glycerin, polyethylene oxide, alum, modified sodium cellulose, a composite plant extract, modified sodium cellulose and other components, and the chitosan gel has antibacterial and anti-inflammatory effects by utilizing good biocompatibility and biodegradability of chitosan, and can inhibit growth and reproduction of bacteria by synergistic cooperation of the components, so that the effects of treating and preventing diseases are achieved. The invention has simple formula, easily obtained raw materials, simple preparation method and stable process, and can be popularized and applied.
Description
Technical Field
The invention relates to the technical field of medical treatment, in particular to chitosan and a preparation method thereof.
Background
Chitosan is obtained by deacetylation of chitin widely existing in the nature, the chemical name is polyglucosamine (1-4) -2-amino-B-D glucose, and since 1859, after the first chitosan is obtained by Rouget of the law, the excellent performances of the natural polymer, such as biological functionality, compatibility, blood compatibility, safety, microbial degradability and the like, are widely concerned by various industries, and the application research in various fields, such as medicine, food, chemical industry, cosmetics, water treatment, metal extraction and recovery, biochemistry, biomedical engineering and the like, has made a great progress. For patients, the effects of chitosan in reducing blood fat and blood sugar have been reported.
Currently, chitosan is widely used in pharmaceutical dosage forms as a formulation adjuvant. Experimental research shows that the chitosan serving as a drug carrier can control drug release, reduce adverse drug reactions and prolong the curative effect of the drug, can improve the permeability of hydrophobic drugs to cell membranes and the stability of the drug, change the administration route and greatly enhance the targeted administration capability of the preparation.
With the continuous development and progress of the bioengineering technology, the separation and purification problems of downstream products of bioengineering become more and more prominent. It is estimated that the capital invested in downstream product manufacturing techniques and associated equipment can account for 2/3 throughout the production of bioengineered products. The biological gel is an essential material for separating and purifying biological products, and has good biological affinity for separating and purifying objects and good separation effect. But the industrial application of the biogel is greatly influenced by the expensive price of the biogel. At present, the gel mostly uses cellulose, glucan and the like as carriers, such as DEAE-cellulose, diethylamino glucan gel and the like, and the carriers have some defects, such as high price, complex synthesis and regeneration and the like. Therefore, the search for gel with proper performance and low price has become the key point for realizing the production of downstream products in bioengineering at present.
Disclosure of Invention
1. Technical problem to be solved
The chitosan gel comprises chitosan, lactic acid solution, glycerol, polyethylene oxide, alum, modified sodium cellulose and other components, and has the antibacterial and anti-inflammatory effects by utilizing the good biocompatibility and biodegradability of chitosan, and the components are cooperatively matched to inhibit the growth and reproduction of bacteria, so that the effects of treating and preventing diseases are achieved.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
A chitosan mainly comprises chitosan gel, wherein the chitosan gel comprises the following components in percentage by mass: 8-12% of chitosan, 5-8% of lactic acid solution, 10-20% of glycerol, 10-20% of polyethylene oxide, 2-5% of alum, 3-6% of modified sodium cellulose, 5-10% of composite plant extract, 0.5-1.5% of modified attapulgite and the balance of deionized water.
Further, the chitosan is chitosan with deacetylation degree of 86-90%.
Further, the modified sodium cellulose is a mixture of sodium carboxymethyl cellulose and sodium carboxymethyl cellulose, and the mass ratio of the sodium carboxymethyl cellulose to the sodium carboxymethyl cellulose is 1: 1-1.5.
Further, the raw materials of the plant composite extract comprise the following components in parts by weight: 10-15 parts of ginseng, 20-30 parts of coptis chinensis, 10-30 parts of radix scutellariae, 10-30 parts of dandelion, 20-40 parts of fig leaves and 10-20 parts of honeysuckle.
Further, the method comprises the following steps: the preparation process of the composite plant extract comprises the following steps: cleaning Ginseng radix, Coptidis rhizoma, Scutellariae radix, herba Taraxaci, folium fici and flos Lonicerae, extracting with deionized water twice, filtering, mixing filtrates, concentrating, and drying to obtain plant extract.
Further, the modified attapulgite is acid modified attapulgite, and the preparation process comprises the following steps: grinding and crushing attapulgite, sieving with a 150-mesh sieve of 100 meshes, placing the attapulgite into a stirring tank, slowly dropwise adding a hydrochloric acid solution with the mass 4-6 times that of the attapulgite, stirring for 1-2h, heating to 45-65 ℃, adding an alkaline solution to adjust the pH value to 5.5-6.5, keeping stirring in the adjusting process, standing and cooling after the adjustment is finished to obtain an attapulgite mixed solution, then carrying out reduced pressure suction filtration on the attapulgite mixed solution, drying for a period of time, crushing and grinding, and sieving with a 400-mesh sieve of 300 meshes to obtain the modified attapulgite.
Further, in the preparation process of the modified attapulgite: the concentration of the hydrochloric acid solution is 8-12%, the drying temperature is 90-120 ℃, and the drying time is 3-4 h.
8. A preparation method of chitosan comprises the following steps:
s1, taking chitosan, lactic acid solution, glycerol, polyethylene oxide, alum, modified sodium cellulose, a composite plant extract, modified attapulgite and deionized water according to mass percentage for later use;
s2, placing the chitosan and lactic acid solution into a mixer, and fully stirring to obtain a mixed glue solution A;
s3, adding glycerol, polyethylene oxide, alum and alum into the mixed glue solution A, adding half of deionized water, and fully stirring to obtain a mixed glue solution B;
s4, adding the composite plant extract and the residual deionized water into the mixed glue solution B, and fully stirring and mixing to obtain a mixed glue solution C;
s5, adding the modified attapulgite into the mixed glue solution C, stirring and mixing, standing, and sterilizing to obtain the chitosan gel.
Further, in the step S5, the sterilization mode is ultraviolet irradiation sterilization, and the sterilization time is 20-30 min. .
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
(1) the chitosan gel comprises components such as chitosan, lactic acid solution, glycerol, polyethylene oxide, alum, modified sodium cellulose and the like, has antibacterial and anti-inflammatory effects by utilizing good biocompatibility and biodegradability of chitosan, and can inhibit bacterial growth and reproduction by synergistic cooperation of the components, thereby playing a role in treating and preventing diseases.
(2) The invention also adds the composite plant extract, the raw materials of the composite plant extract adopt the combination of ginseng, coptis, radix scutellariae, dandelion, fig leaves and honeysuckle, and all the components have certain antibacterial and anti-inflammatory effects, thereby further improving the antibacterial effect of the invention and ensuring the use effect.
(3) The modified attapulgite is added, so that the smoothness, the sealing property and the air permeability of the modified attapulgite can be improved, the specific surface area of the modified attapulgite is obviously improved, and the adsorption property and the adhesiveness are improved.
(4) The invention has simple formula, easily obtained raw materials, simple preparation method and stable process, and can be popularized and applied.
Drawings
FIG. 1 is a flow chart of the preparation method of the present invention.
Detailed Description
The drawings in the embodiments of the invention will be combined; the technical scheme in the embodiment of the invention is clearly and completely described; obviously; the described embodiments are only some of the embodiments of the invention; but not all embodiments, are based on the embodiments of the invention; all other embodiments obtained by a person skilled in the art without making any inventive step; all fall within the scope of protection of the present invention.
Example 1:
a chitosan mainly comprises chitosan gel, wherein the chitosan gel comprises the following components in percentage by mass: 8% of chitosan, 5% of lactic acid solution, 10% of glycerol, 10% of polyethylene oxide, 2% of alum, 3% of modified sodium cellulose, 5% of composite plant extract, 0.5% of modified attapulgite and the balance of deionized water.
The chitosan is chitosan with deacetylation degree of 86%.
The modified sodium cellulose is a mixture of sodium carboxymethyl cellulose and sodium carboxymethyl cellulose, and the mass ratio of the sodium carboxymethyl cellulose to the sodium carboxymethyl cellulose is 1: 1.
the raw materials of the plant composite extract comprise the following components in parts by weight: 10 parts of ginseng, 20 parts of coptis chinensis, 10 parts of radix scutellariae, 10 parts of dandelion, 20 parts of fig leaves and 10 parts of honeysuckle.
The preparation process of the composite plant extract comprises the following steps: cleaning Ginseng radix, Coptidis rhizoma, Scutellariae radix, herba Taraxaci, folium fici and flos Lonicerae, extracting with deionized water twice, filtering, mixing filtrates, concentrating, and drying to obtain plant extract.
The modified attapulgite is acid modified attapulgite, and the preparation process comprises the following steps: grinding and crushing attapulgite, sieving with a 100-mesh sieve, putting the attapulgite into a stirring tank, slowly dropwise adding a hydrochloric acid solution with the mass 4 times that of the attapulgite, stirring for 1h, heating to 45 ℃, adding an alkaline solution to adjust the pH value to 5.5, keeping stirring in the adjusting process, standing and cooling after the adjustment is finished to obtain an attapulgite mixed solution, then carrying out reduced pressure suction filtration on the attapulgite mixed solution, drying for a period of time, crushing and grinding, and sieving with a 300-mesh sieve to obtain the modified attapulgite.
The preparation process of the modified attapulgite comprises the following steps: the concentration of the hydrochloric acid solution is 8 percent, the drying temperature is 90 ℃, and the drying time is 3 hours.
Example 2:
a chitosan mainly comprises chitosan gel, wherein the chitosan gel comprises the following components in percentage by mass: 10% of chitosan, 6% of lactic acid solution, 15% of glycerol, 15% of polyethylene oxide, 3% of alum, 5% of modified sodium cellulose, 7% of composite plant extract, 1% of modified attapulgite and the balance of deionized water.
The chitosan is chitosan with deacetylation degree of 88%.
The modified sodium cellulose is a mixture of sodium carboxymethyl cellulose and sodium carboxymethyl cellulose, and the mass ratio of the sodium carboxymethyl cellulose to the sodium carboxymethyl cellulose is 1: 1.2.
the raw materials of the plant composite extract comprise the following components in parts by weight: 12 parts of ginseng, 25 parts of coptis chinensis, 20 parts of radix scutellariae, 20 parts of dandelion, 30 parts of fig leaves and 15 parts of honeysuckle.
The preparation process of the composite plant extract comprises the following steps: cleaning Ginseng radix, Coptidis rhizoma, Scutellariae radix, herba Taraxaci, folium fici and flos Lonicerae, extracting with deionized water twice, filtering, mixing filtrates, concentrating, and drying to obtain plant extract.
The modified attapulgite is acid modified attapulgite, and the preparation process comprises the following steps: grinding and crushing attapulgite, sieving with a 125-mesh sieve, putting the attapulgite into a stirring tank, slowly dropwise adding a hydrochloric acid solution with the mass 5 times that of the attapulgite, stirring for 1.5h, heating to 55 ℃, adding an alkaline solution to adjust the pH value to 6, keeping stirring in the adjusting process, standing and cooling after the adjustment is finished to obtain an attapulgite mixed solution, then carrying out reduced pressure suction filtration on the attapulgite mixed solution, drying for a period of time, crushing and grinding, and sieving with a 350-mesh sieve to obtain the modified attapulgite.
The preparation process of the modified attapulgite comprises the following steps: the concentration of the hydrochloric acid solution is 10 percent, the drying temperature is 100 ℃, and the drying time is 3.5 h.
Example 3:
a chitosan mainly comprises chitosan gel, wherein the chitosan gel comprises the following components in percentage by mass: 12% of chitosan, 8% of lactic acid solution, 20% of glycerol, 20% of polyethylene oxide, 5% of alum, 6% of modified sodium cellulose, 10% of composite plant extract, 1.5% of modified attapulgite and the balance of deionized water.
The chitosan is chitosan with deacetylation degree of 90%.
The modified sodium cellulose is a mixture of sodium carboxymethyl cellulose and sodium carboxymethyl cellulose, and the mass ratio of the sodium carboxymethyl cellulose to the sodium carboxymethyl cellulose is 1: 1.5.
the raw materials of the plant composite extract comprise the following components in parts by weight: 15 parts of ginseng, 30 parts of coptis chinensis, 30 parts of radix scutellariae, 30 parts of dandelion, 40 parts of fig leaves and 20 parts of honeysuckle.
The preparation process of the composite plant extract comprises the following steps: cleaning Ginseng radix, Coptidis rhizoma, Scutellariae radix, herba Taraxaci, folium fici and flos Lonicerae, extracting with deionized water twice, filtering, mixing filtrates, concentrating, and drying to obtain plant extract.
The modified attapulgite is acid modified attapulgite, and the preparation process comprises the following steps: grinding and crushing attapulgite, sieving with a 150-mesh sieve, putting the attapulgite into a stirring tank, slowly dropwise adding a hydrochloric acid solution with the mass 6 times that of the attapulgite, stirring for 2 hours, heating to 65 ℃, adding an alkaline solution to adjust the pH value to 6.5, keeping stirring in the adjusting process, standing and cooling after the adjustment is finished to obtain an attapulgite mixed solution, then carrying out reduced pressure suction filtration on the attapulgite mixed solution, drying for a period of time, crushing and grinding, and sieving with a 400-mesh sieve to obtain the modified attapulgite.
The preparation process of the modified attapulgite comprises the following steps: the concentration of the hydrochloric acid solution is 12 percent, the drying temperature is 120 ℃, and the drying time is 4 hours.
Example 4
Referring to fig. 1, a method for preparing chitosan includes the following steps:
s1, taking chitosan, lactic acid solution, glycerol, polyethylene oxide, alum, modified sodium cellulose, a composite plant extract, modified attapulgite and deionized water according to mass percentage for later use;
s2, placing the chitosan and lactic acid solution into a mixer, and fully stirring to obtain a mixed glue solution A;
s3, adding glycerol, polyethylene oxide, alum and alum into the mixed glue solution A, adding half of deionized water, and fully stirring to obtain a mixed glue solution B;
s4, adding the composite plant extract and the residual deionized water into the mixed glue solution B, and fully stirring and mixing to obtain a mixed glue solution C;
s5, adding the modified attapulgite into the mixed glue solution C, stirring and mixing, standing, and sterilizing to obtain the chitosan gel.
In S5, the sterilization mode is ultraviolet irradiation sterilization, and the sterilization time is 20-30 min.
The above; but are merely preferred embodiments of the invention; the scope of the invention is not limited thereto; any person skilled in the art is within the technical scope of the present disclosure; the technical scheme and the improved concept of the invention are equally replaced or changed; are intended to be covered by the scope of the present invention.
Claims (9)
1. A chitosan, the main component of which is chitosan gel, is characterized in that: the chitosan gel comprises the following components in percentage by mass: 8-12% of chitosan, 5-8% of lactic acid solution, 10-20% of glycerol, 10-20% of polyethylene oxide, 2-5% of alum, 3-6% of modified sodium cellulose, 5-10% of composite plant extract, 0.5-1.5% of modified attapulgite and the balance of deionized water.
2. A chitosan according to claim 1, wherein: the chitosan is chitosan with deacetylation degree of 86-90%.
3. A chitosan according to claim 1, wherein: the modified sodium cellulose is a mixture of sodium carboxymethyl cellulose and sodium carboxymethyl cellulose, and the mass ratio of the sodium carboxymethyl cellulose to the sodium carboxymethyl cellulose is 1: 1-1.5.
4. A chitosan according to claim 1, wherein: the plant composite extract comprises the following raw materials in parts by weight: 10-15 parts of ginseng, 20-30 parts of coptis chinensis, 10-30 parts of radix scutellariae, 10-30 parts of dandelion, 20-40 parts of fig leaves and 10-20 parts of honeysuckle.
5. A chitosan according to claim 1, wherein: the preparation process of the composite plant extract comprises the following steps: cleaning Ginseng radix, Coptidis rhizoma, Scutellariae radix, herba Taraxaci, folium fici and flos Lonicerae, extracting with deionized water twice, filtering, mixing filtrates, concentrating, and drying to obtain plant extract.
6. A chitosan according to claim 1, wherein: the modified attapulgite is acid modified attapulgite, and the preparation process comprises the following steps: grinding and crushing attapulgite, sieving with a 150-mesh sieve of 100 meshes, placing the attapulgite into a stirring tank, slowly dropwise adding a hydrochloric acid solution with the mass 4-6 times that of the attapulgite, stirring for 1-2h, heating to 45-65 ℃, adding an alkaline solution to adjust the pH value to 5.5-6.5, keeping stirring in the adjusting process, standing and cooling after the adjustment is finished to obtain an attapulgite mixed solution, then carrying out reduced pressure suction filtration on the attapulgite mixed solution, drying for a period of time, crushing and grinding, and sieving with a 400-mesh sieve of 300 meshes to obtain the modified attapulgite.
7. A chitosan according to claim 1, wherein: the preparation process of the modified attapulgite comprises the following steps: the concentration of the hydrochloric acid solution is 8-12%, the drying temperature is 90-120 ℃, and the drying time is 3-4 h.
8. A method for preparing chitosan according to any one of claims 1-7, comprising the steps of:
s1, taking chitosan, lactic acid solution, glycerol, polyethylene oxide, alum, modified sodium cellulose, a composite plant extract, modified attapulgite and deionized water according to mass percentage for later use;
s2, placing the chitosan and lactic acid solution into a mixer, and fully stirring to obtain a mixed glue solution A;
s3, adding glycerol, polyethylene oxide, alum and alum into the mixed glue solution A, adding half of deionized water, and fully stirring to obtain a mixed glue solution B;
s4, adding the composite plant extract and the residual deionized water into the mixed glue solution B, and fully stirring and mixing to obtain a mixed glue solution C;
s5, adding the modified attapulgite into the mixed glue solution C, stirring and mixing, standing, and sterilizing to obtain the chitosan gel.
9. The method of claim 1, wherein the chitosan is prepared by the following steps: in the step S5, the sterilization mode is ultraviolet irradiation sterilization, and the sterilization time is 20-30 min.
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