CN111664249B - A sealing device for vertical electrophoresis device - Google Patents
A sealing device for vertical electrophoresis device Download PDFInfo
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- CN111664249B CN111664249B CN201910170812.3A CN201910170812A CN111664249B CN 111664249 B CN111664249 B CN 111664249B CN 201910170812 A CN201910170812 A CN 201910170812A CN 111664249 B CN111664249 B CN 111664249B
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- buckle
- fixing frame
- gel
- movable
- sealing strip
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16J—PISTONS; CYLINDERS; SEALINGS
- F16J15/00—Sealings
- F16J15/02—Sealings between relatively-stationary surfaces
- F16J15/06—Sealings between relatively-stationary surfaces with solid packing compressed between sealing surfaces
- F16J15/061—Sealings between relatively-stationary surfaces with solid packing compressed between sealing surfaces with positioning means
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16J—PISTONS; CYLINDERS; SEALINGS
- F16J15/00—Sealings
- F16J15/02—Sealings between relatively-stationary surfaces
- F16J15/06—Sealings between relatively-stationary surfaces with solid packing compressed between sealing surfaces
- F16J15/062—Sealings between relatively-stationary surfaces with solid packing compressed between sealing surfaces characterised by the geometry of the seat
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F16—ENGINEERING ELEMENTS AND UNITS; GENERAL MEASURES FOR PRODUCING AND MAINTAINING EFFECTIVE FUNCTIONING OF MACHINES OR INSTALLATIONS; THERMAL INSULATION IN GENERAL
- F16J—PISTONS; CYLINDERS; SEALINGS
- F16J15/00—Sealings
- F16J15/02—Sealings between relatively-stationary surfaces
- F16J15/06—Sealings between relatively-stationary surfaces with solid packing compressed between sealing surfaces
- F16J15/10—Sealings between relatively-stationary surfaces with solid packing compressed between sealing surfaces with non-metallic packing
- F16J15/104—Sealings between relatively-stationary surfaces with solid packing compressed between sealing surfaces with non-metallic packing characterised by structure
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
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- General Engineering & Computer Science (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Mechanical Engineering (AREA)
- Health & Medical Sciences (AREA)
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- Molecular Biology (AREA)
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- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Geometry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
本发明公开了一种用于垂直电泳装置的密封装置。所述密封装置包括：垂直电泳装置主体、夹紧板以及密封底座；垂直电泳装置主体包括固定架以及DNA分子片段检测腔；固定架包括第一固定架以及第二固定架；DNA分子片段检测腔设于所述第一固定架与所述第二固定架之间；第一固定架的上表面以及下表面为“山”字形结构，形成两个间隙；第一固定架包括第一侧面、第二侧面以及第三侧面；第一侧面与第二侧面相同，均为U型结构，U型结构两端之间设有夹紧板；夹紧板与U型结构的两端转动连接；第三侧面设有拉紧凸点；拉紧凸点用于将密封底座紧扣于所述固定架上。采用本发明所提供的用于垂直电泳装置的密封装置，能够有效防止凝胶制备过程中出现漏胶的问题。
The present invention discloses a sealing device for a vertical electrophoresis device. The sealing device comprises: a vertical electrophoresis device body, a clamping plate and a sealing base; the vertical electrophoresis device body comprises a fixing frame and a DNA molecule fragment detection cavity; the fixing frame comprises a first fixing frame and a second fixing frame; the DNA molecule fragment detection cavity is arranged between the first fixing frame and the second fixing frame; the upper surface and the lower surface of the first fixing frame are "mountain" shaped structures, forming two gaps; the first fixing frame comprises a first side surface, a second side surface and a third side surface; the first side surface is the same as the second side surface, both of which are U-shaped structures, and a clamping plate is arranged between the two ends of the U-shaped structure; the clamping plate is rotatably connected to the two ends of the U-shaped structure; the third side surface is provided with a tightening protrusion; the tightening protrusion is used to fasten the sealing base to the fixing frame. The sealing device for a vertical electrophoresis device provided by the present invention can effectively prevent the problem of gel leakage during gel preparation.
Description
Technical Field
The invention relates to the technical field of equipment for separating and detecting DNA molecules, in particular to a sealing device for a vertical electrophoresis device.
Background
The vertical electrophoresis device is an instrument for separating and detecting DNA molecules, and before separating and detecting DNA molecules, agar gel is generally used as a support, and the purpose of separating a mixture is achieved by utilizing the charge effect and the molecular sieve effect of the DNA molecules during electrophoresis.
Agarose gel is commonly used as a support for separating and identifying a mixture of DNA and RNA molecules, and in order to increase the detection speed of a vertical electrophoresis device and to make the agarose gel more fit with the vertical electrophoresis device, the preparation of the agarose gel is usually carried out directly on the vertical electrophoresis device, and as the gel substance of the agarose gel is liquid, the problem of gel leakage often occurs in the gel preparation process, so that the gel is wasted.
Disclosure of Invention
The invention aims to provide a sealing device for a vertical electrophoresis device, which solves the problem that glue leakage frequently occurs in the existing gel preparation process.
In order to achieve the above object, the present invention provides the following solutions:
A sealing device for a vertical electrophoresis apparatus includes a vertical electrophoresis apparatus body, a clamping plate, and a sealing base;
The vertical electrophoresis device main body comprises a fixing frame and a DNA molecular fragment detection cavity, wherein the fixing frame comprises a first fixing frame and a second fixing frame, the first fixing frame and the second fixing frame have the same structure, and the DNA molecular fragment detection cavity is arranged between the first fixing frame and the second fixing frame;
The upper surface and the lower surface of the first fixing frame are of a mountain-shaped structure to form two gaps, the gaps are used for placing a gel preparation device, the first fixing frame comprises a first side face, a second side face and a third side face, the first side face is identical to the second side face and is of a U-shaped structure, clamping plates are arranged between two ends of the U-shaped structure and are in rotary connection with two ends of the U-shaped structure, the third side face is provided with tightening protruding points, and the tightening protruding points are used for tightly fastening the sealing base on the fixing frame so that the sealing base is tightly attached to the gel preparation device.
Optionally, the sealing base comprises a glue making base, a glue making sealing strip and a fixing buckle;
The upper surface of the glue making seat is provided with the fixing buckle, the fixing buckle comprises a first fixing buckle and a second fixing buckle, the glue making sealing strip is arranged between the first fixing buckle and the second fixing buckle, the glue making sealing strip is arranged on the glue making seat, and the fixing buckle is used for clamping the vertical electrophoresis device.
Optionally, the fixed buckle comprises a vertical end, a movable buckle and a tensioning buckle;
The vertical end is fixed on the glue making seat, the vertical end is rotationally connected with the movable buckle, and the movable buckle is rotationally connected with the tensioning buckle.
Optionally, the device also comprises a pin shaft;
The pin shaft sequentially penetrates through one side of the movable buckle, the vertical end and the other side of the movable buckle, so that the vertical end is rotationally connected with the movable buckle, and the movable buckle rotates through the pin shaft to drive the tensioning buckle to move.
Optionally, a movable groove is arranged at the rotary connection part of the movable buckle and the tensioning buckle, and the movable groove comprises a first movable groove and a second movable groove;
The inner side of the tensioning buckle is provided with a movable protruding point, the movable protruding point comprises a first movable protruding point and a second movable protruding point, the first movable protruding point is matched with the first movable groove, the second movable protruding point is matched with the second movable groove, and the movable groove and the movable protruding point are used for rotationally connecting the tensioning buckle with the movable buckle.
Optionally, a seesaw is arranged at the bottom end of the tensioning buckle;
In a tensioned state that the tensioning buckle is fastened to the vertical electrophoresis device, the seesaw is parallel to the vertical end.
Optionally, the sealing strip comprises a first sealing strip and a second sealing strip;
the first glue making sealing strip is parallel to the second glue making sealing strip, the width of the first glue making sealing strip is equal to that of the second glue making sealing strip, and the width of the first glue making sealing strip is larger than that of the gel preparation device.
Optionally, the utility model also comprises a ground leg;
the lower margin is arranged at the bottom of the glue making seat and is used for supporting and fixing the sealing base.
According to the specific embodiment provided by the invention, the sealing device for the vertical electrophoresis device has the following technical effects that the side surface and the bottom surface of the gel preparation device are completely sealed through the clamping plate and the sealing base, so that the problem of glue leakage in the gel preparation process is effectively prevented.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions of the prior art, the drawings that are needed in the embodiments will be briefly described below, it being obvious that the drawings in the following description are only some embodiments of the present invention, and that other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a front view of a sealing device of a vertical electrophoresis device according to the present invention;
fig. 2 is a top view of a sealing device of the vertical electrophoresis device according to the present invention;
FIG. 3 is a D-D sectional view of a sealing device of a vertical electrophoresis device according to the present invention;
fig. 4 is a perspective view of a vertical electrophoresis apparatus in a clamped state of a clamping plate according to the present invention;
fig. 5 is a front view of the seal base provided by the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention aims to provide a sealing device for a vertical electrophoresis device, which can effectively prevent the problem of glue leakage in the gel preparation process.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
Fig. 1 is a front view of a sealing device of a vertical electrophoresis device, as shown in fig. 1, and is a cross-sectional view of a sealing device D-D of the vertical electrophoresis device, as shown in fig. 2-3, wherein the vertical electrophoresis device body 1 comprises a fixing frame 4 and a DNA molecule fragment detection cavity 5, the fixing frame 4 comprises a first fixing frame and a second fixing frame, the first fixing frame and the second fixing frame are identical in structure, the DNA molecule fragment detection cavity 5 is arranged between the first fixing frame and the second fixing frame, fig. 2 is a top view of the sealing device of the vertical electrophoresis device, as shown in fig. 2-3, the upper surface and the lower surface of the first fixing frame are of a 'mountain' -shaped structure to form two gaps, the gaps are used for placing gel preparation devices, the first fixing frame comprises a first side face 4-1, a second side face 4-2 and a third side face 4-3, the first side face 4-1 is arranged between the first fixing frame and the second fixing frame, the second side face 4-2 is arranged between the first side face and the second fixing frame, the first side face 4-1 and the second side face 4-3 is arranged between the first side face and the second fixing frame, the first side face 4-1 and the second side face 4-2 is provided with a tightening bump, the U-shaped structure is arranged between the first side face and the U-shaped structure and the second side face 4-3 is provided with a tightening bump, and the U-shaped structure is arranged between the U-shaped structure and the U-shaped sealing device is provided with a tightening base, and the U-shaped structure is provided with a tightening bump 4 is formed between the U-shaped structure and the sealing structure is provided with a sealing structure.
The invention further comprises an electrode plug 7 and a cross groove pan head screw 8, wherein the electrode plug is arranged at the top of the fixed frame 4 through a gasket 9, the polarity of the electrode plug 7 on the first fixed frame is positive, the polarity of the electrode plug 7 on the second fixed frame is negative, electrode wires 10 are arranged in the first fixed frame and the second fixed frame, the electrode wires 10 are connected with the electrode plug, a platinum wire sheath 11 is wrapped on the outer surface of the electrode wires 10, and the fixed frame 4 and the DNA molecular fragment detection cavity 5 are fixed together through the cross groove pan head screw 8.
Fig. 4 is a perspective view of the vertical electrophoresis apparatus in the clamped state of the clamping plate according to the present invention, as shown in fig. 4, it can be seen from fig. 4 that the side leakage problem of the gel preparation apparatus can be effectively prevented by the clamping plate 2.
Fig. 5 is a front view of a sealing base provided by the invention, as shown in fig. 5, the sealing base 3 specifically comprises a glue making sealing strip 3-1, a glue making base 3-2 and a fixing buckle, the fixing buckle is arranged on the upper surface of the glue making base 3-2, the fixing buckle comprises a first fixing buckle 3-3-1 and a second fixing buckle 3-3-2, fig. 2 is a top view of the sealing base for the vertical electrophoresis device, as shown in fig. 2, the glue making sealing strip 3-1 is arranged between the first fixing buckle 3-3-1 and the second fixing buckle 3-3-2, the glue making sealing strip 3-1 is arranged on the glue making base 3-2, and the fixing buckle is used for clamping the vertical electrophoresis device.
In practical application, the fixed buckle comprises a vertical end 3-4, a movable buckle 3-5 and a tensioning buckle 3-6, wherein the vertical end 3-4 is fixed on the glue making seat 3-2, the vertical end 3-4 is in rotary connection with the movable buckle 3-5, and the movable buckle 3-5 is in rotary connection with the tensioning buckle 3-6.
In practical application, the sealing base 3 further comprises a pin shaft 3-7, the pin shaft 3-7 sequentially penetrates through one side of the movable buckle 3-5, the vertical end 3-4 and the other side of the movable buckle 3-5, so that the vertical end 3-4 is in rotary connection with the movable buckle 3-5, and the movable buckle 3-5 rotates through the pin shaft 3-7 to drive the tensioning buckle 3-6 to move.
In practical application, a movable groove is formed in the rotary connection position of the movable buckle 3-5 and the tensioning buckle 3-6, the movable groove comprises a first movable groove 3-8-1 and a second movable groove 3-8-2, movable protruding points are arranged on the inner side of the tensioning buckle 3-6 and comprise a first movable protruding point and a second movable protruding point, the first movable protruding point is matched with the first movable groove 3-8-1, the second movable protruding point is matched with the second movable groove 3-8-2, and the movable groove and the movable protruding points are used for rotationally connecting the tensioning buckle 3-6 and the movable buckle 3-5.
In practical application, the bottom end of the tightening buckle 3-6 is provided with a rocker 3-9, the rocker 3-9 is parallel to the vertical end 3-4 in a tightening state that the tightening buckle 3-6 is fastened to the vertical electrophoresis device, the tightening buckle 3-6 is matched with the tightening convex point 6, the rocker 3-9 is used for closing or separating the tightening buckle 3-6 and the tightening convex point 6, and the tightening buckle 3-6 and the movable buckle 3-5 are mutually driven, so that the tightening or separation between the sealing base 3 and the fixing frame 4 is realized.
In practical application, the glue sealing strip 3-1 comprises a first glue sealing strip and a second glue sealing strip, wherein the first glue sealing strip and the second glue sealing strip are parallel to each other, the width of the first glue sealing strip is equal to that of the second glue sealing strip, and the width of the first glue sealing strip is larger than that of the gel preparation device.
The gel preparation device comprises a high glass plate 12 and a low glass plate 13, a gel gap is arranged between the high glass plate 12 and the low glass plate 13, the low glass plate 13 is close to the DNA molecular fragment detection cavity 5, and the width of the gel gap is smaller than that of the gel preparation sealing strip 3-1.
The bottom of the DNA molecule fragment detection cavity 5 is also provided with a U-shaped adhesive tape 14.
The invention further comprises a ground foot 3-11, wherein the ground foot 3-11 is arranged at the bottom of the glue making seat 3-2, and the ground foot 3-11 is used for supporting and fixing the sealing base 3.
According to the invention, the gel preparation device is clamped by the clamping plate 2, so that the problem of gel side leakage of the gel preparation device is effectively prevented, and the sealing base 3 and the fixing frame 4 are tightly fixed together by the fixing buckle in the sealing base 3, so that the gel preparation sealing strip is tightly combined with the gel preparation device, the problem of gel bottom leakage of the gel preparation device is effectively prevented, and the problem of gel leakage of the gel preparation device is further comprehensively and effectively prevented.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The principles and embodiments of the present invention have been described herein with reference to specific examples, which are intended to facilitate an understanding of the principles and concepts of the invention and are to be varied in scope and detail by persons of ordinary skill in the art based on the teachings herein. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910170812.3A CN111664249B (en) | 2019-03-07 | 2019-03-07 | A sealing device for vertical electrophoresis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910170812.3A CN111664249B (en) | 2019-03-07 | 2019-03-07 | A sealing device for vertical electrophoresis device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111664249A CN111664249A (en) | 2020-09-15 |
| CN111664249B true CN111664249B (en) | 2024-12-20 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910170812.3A Active CN111664249B (en) | 2019-03-07 | 2019-03-07 | A sealing device for vertical electrophoresis device |
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| Country | Link |
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| CN (1) | CN111664249B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6193868B1 (en) * | 1999-09-16 | 2001-02-27 | Yi-Hua Hsu | Electrophoretic separating and blotting apparatus |
| CN102900291A (en) * | 2012-10-09 | 2013-01-30 | 禚度鹏 | Lock catch for pigsty |
| CN204302224U (en) * | 2014-10-28 | 2015-04-29 | 黄志春 | Glue and vertical electrophoresis fexible unit |
| CN209587129U (en) * | 2019-03-07 | 2019-11-05 | 北京六一生物科技有限公司 | A kind of sealing device for vertical electrophoresis device |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3932265A (en) * | 1974-03-28 | 1976-01-13 | Hoefer Scientific Instruments | Vertical gel slab electrophoresis apparatus |
| US4290871A (en) * | 1979-01-03 | 1981-09-22 | Hoefer Scientific Instruments | Vertical gel slab electrophoresis method |
| US6513813B1 (en) * | 2000-05-24 | 2003-02-04 | Large Scale Proteomics Corporation | Gel slab sealing strip for an electrophoresis tank |
| DE20115190U1 (en) * | 2001-09-14 | 2002-03-28 | Wealtec Enterprise Co., Ltd., Lu-Chou, Taipeh | Elektrophoreseabscheider |
| US6942775B1 (en) * | 2002-05-24 | 2005-09-13 | Owl Separation Systems, Inc. | Vertical electrophoresis system |
| US7276143B2 (en) * | 2003-11-17 | 2007-10-02 | Hui-Wan Chen | Electrophoresis module having electrophoresis bath with upright carriers |
| CN2824030Y (en) * | 2005-09-29 | 2006-10-04 | 北京百晶生物技术有限公司 | Vertical electrophoresis apparatus of once clamping gel chamber |
| CN202851951U (en) * | 2012-11-07 | 2013-04-03 | 黑龙江八一农垦大学 | Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis bottom leak-proof sealing device |
| CN206159466U (en) * | 2016-07-05 | 2017-05-10 | 长江大学 | Bottom leakproof seal device of frame is glued to gel system |
| CN206132703U (en) * | 2016-07-27 | 2017-04-26 | 北京六一生物科技有限公司 | Perpendicular electrophoresis apparatus of four boards is glued to normal position system |
-
2019
- 2019-03-07 CN CN201910170812.3A patent/CN111664249B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6193868B1 (en) * | 1999-09-16 | 2001-02-27 | Yi-Hua Hsu | Electrophoretic separating and blotting apparatus |
| CN102900291A (en) * | 2012-10-09 | 2013-01-30 | 禚度鹏 | Lock catch for pigsty |
| CN204302224U (en) * | 2014-10-28 | 2015-04-29 | 黄志春 | Glue and vertical electrophoresis fexible unit |
| CN209587129U (en) * | 2019-03-07 | 2019-11-05 | 北京六一生物科技有限公司 | A kind of sealing device for vertical electrophoresis device |
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| CN111664249A (en) | 2020-09-15 |
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