CN111657262A - Organ preservation device and organ preservation method - Google Patents

Abstract

The present application provides an organ preservation device and an organ preservation method, which belong to the technical field of organ preservation. The organ preservation device includes: a controller, a transfer tank, a recovery tank, a fluid replacement component, a gas supplementation component, a gas-liquid mixing component, a perfusion component, a return component, and a box body; the controller is used to: control the start of the fluid replacement component to send the fluid to the transfer tank. Supply nutrient solution; control the start of the gas supply component to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component; control the start of the perfusion component to pump the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing component, and The gas-liquid output from the gas-liquid output end of the gas-liquid mixing component is perfused to the arteries of the organ; the backflow component is controlled to be activated to return the liquid in the recovery tank to the transfer tank. The present application solves the problem that the organ preservation time needs to be further improved. This application is for organ preservation.

Description

Organ preservation device and organ preservation method

technical field

The present application relates to the technical field of organ preservation, and in particular, to an organ preservation device and an organ preservation method.

Background technique

In medical research and clinical processes involving organs, organs are used, and certain preservation techniques are used to preserve the organs.

For example, in the process of organ transplantation, after the donor organ is obtained, the donor organ needs to be preserved to ensure the integrity and health of the donor organ. Currently, organs are typically preserved using cryopreservation techniques or cryopreservation techniques, which preserve organs by reducing their temperature in a way that reduces organ viability.

However, using this type of technology to preserve organs will cause ice crystal damage to the organ, and long-term preservation will cause damage to the organ function. It is not suitable to preserve the organ for a long time. The preservation time of the current organ preservation technology needs to be further improved.

SUMMARY OF THE INVENTION

The application provides an organ preservation device and an organ preservation method, which can solve the problem that the preservation time of the organ preservation technology in the related art needs to be further improved. The technical solution is as follows:

In one aspect, an organ preservation device is provided, the organ preservation device comprising: a controller, a transfer tank, a recovery tank, a fluid supplement component, a gas supplement component, a gas-liquid mixing component, a perfusion component, a return component, and a box;

The transfer tank and the recovery tank are both located in the box, the recovery tank is used for placing organs, the fluid replacement component is communicated with the transfer tank, and the gas-liquid input end of the gas-liquid mixing component is connected to the The gas supply component and the transfer tank are both connected, the gas-liquid output end of the gas-liquid mixing component is connected with the perfusion component, the perfusion component is also connected with the artery of the organ, and the return component is connected with the recovery component tank and the transfer tank; the controller is used for:

controlling the start of the liquid replenishing component to supply nutrient solution to the transfer tank;

controlling the start of the gas supply component to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component;

Control the perfusion component to start, to pump the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing component, and perfuse the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component to the arteries of said organ;

Control the activation of the return component to return the liquid in the recovery tank to the transfer tank.

Optionally, the organ preservation device further comprises: a transfer liquid level monitoring component, the transfer liquid level monitoring component is fixed on the side wall of the transfer tank, and the controller is further configured to:

controlling the transfer liquid level monitoring component to monitor the liquid level of the liquid in the transfer tank;

After the liquid level of the liquid in the transfer tank reaches the transfer low level, controlling the gas supplementation component and the perfusion component to start, and the perfusion rate of the perfusion component is less than the liquid supplementation rate of the liquid supplementation component;

When the liquid level of the liquid in the transfer tank reaches the transfer high level, the liquid replenishing component is controlled to suspend;

During the operation of the return component, when the liquid level of the liquid in the transfer tank reaches the high transfer level or is lower than the low transfer level, the return component is adjusted to return the liquid in the recovery tank to the desired level. The speed of the transfer tank is increased until the liquid level of the liquid in the transfer tank is lower than the transfer high level and higher than the transfer low level.

Optionally, the organ preservation device further includes: a nutrient solution monitoring component, a recovery liquid level monitoring component, a waste liquid tank, and a waste liquid discharge component, wherein a mesh cover is fixedly arranged in the recovery tank, and the mesh cover is used to carry the load. The organ, the nutrient solution monitoring component is located in the transfer tank, the recovery liquid level monitoring component is fixed on the side wall of the recovery tank, and the waste liquid discharge component communicates with the recovery tank and the waste liquid tank, the controller is also used to:

controlling the recovery liquid level monitoring component to monitor the liquid level of the liquid in the recovery tank;

During the operation of the return component, when the liquid level of the liquid in the recovery tank reaches a high recovery level, adjust the rate at which the return component returns the liquid in the recovery tank to the transfer tank until the The liquid level of the liquid in the transfer tank is lower than the recovery high level;

Controlling the nutrient solution monitoring component to monitor the concentration of a given nutrient substance in the liquid in the transfer tank;

When the concentration of a given nutrient substance in the liquid in the transfer tank is lower than a given concentration, the backflow component is controlled to suspend, and the waste liquid discharge component is controlled to start, so as to discharge the liquid in the recovery tank into the waste tank;

During the operation of the waste liquid discharge assembly, when the liquid level of the liquid in the transfer tank is lower than the transfer low level and the liquid level of the liquid in the recovery tank is lower than the recovery low level, the waste liquid is controlled to discharge The assembly is suspended, and the height of the position of the recovery high position and the height of the position of the recovery low position are both lower than the height of the position of the grille;

The liquid replenishing assembly is controlled to start, and when the liquid level of the liquid in the transfer tank reaches the transfer high level, the liquid replenishing assembly is controlled to suspend, and the return flow assembly is controlled to start.

Optionally, the nutrient gas includes oxygen, and the organ preservation device further includes: a liquid-gas analyzer, the liquid-gas analyzer is communicated with the gas-liquid output end of the gas-liquid mixing assembly, and the controller also uses At:

During the operation of the air supply component, controlling the air supply component to supply oxygen to the gas-liquid input end of the gas-liquid mixing component;

controlling the liquid-gas analyzer to monitor the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly;

When the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly exceeds a given oxygen partial pressure range, the air-supplying assembly is adjusted to supply oxygen to the gas-liquid input end of the gas-liquid mixing assembly until the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component is restored to within the given oxygen partial pressure range.

Optionally, the nutrient gas includes carbon dioxide, and the controller is further configured to:

During the operation of the gas supplement component, controlling the gas supplement component to supplement carbon dioxide to the gas-liquid input end of the gas-liquid mixing component;

Controlling the liquid-gas analyzer to monitor the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly;

When the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component exceeds a given pH range, adjust the rate of supplying carbon dioxide from the gas supply component to the gas-liquid input end of the gas-liquid mixing component until The pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly is restored to the given pH range.

On the other hand, an organ preservation method is provided, the organ preservation method is used for the controller in the above-mentioned organ preservation device, and the organ preservation device further comprises: a transfer tank, a recovery tank, a liquid supplement component, a gas supplement component, Gas-liquid mixing components, perfusion components, return components and boxes;

The transfer tank and the recovery tank are both located in the box, the recovery tank is used for placing organs, the fluid replacement component is communicated with the transfer tank, and the gas-liquid input end of the gas-liquid mixing component is connected to the The gas supply component and the transfer tank are both connected, the gas-liquid output end of the gas-liquid mixing component is connected with the perfusion component, the perfusion component is also connected with the artery of the organ, and the return component is connected with the recovery component tank and the transfer tank; the method includes:

controlling the start of the liquid replenishing component to supply nutrient solution to the transfer tank;

controlling the start of the gas supply component to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component;

Control the perfusion component to start, to pump the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing component, and perfuse the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component to the arteries of said organ;

Control the activation of the return component to return the liquid in the recovery tank to the transfer tank.

Optionally, the organ preservation device further includes: a transfer liquid level monitoring assembly, the transfer liquid level monitoring assembly being fixed on the side wall of the transfer tank;

After the control of the rehydration component is started, the method further includes:

controlling the transfer liquid level monitoring component to monitor the liquid level of the liquid in the transfer tank;

After the liquid level of the liquid in the transfer tank reaches the transfer low level, controlling the gas supplementation component and the perfusion component to start, and the perfusion rate of the perfusion component is less than the liquid supplementation rate of the liquid supplementation component;

When the liquid level of the liquid in the transfer tank reaches the transfer high level, the liquid replenishing component is controlled to suspend;

After the control of the reflow assembly is started, the method further includes:

When the liquid level of the liquid in the transfer tank reaches the transfer high level or is lower than the transfer low level, adjust the rate at which the return component returns the liquid in the recovery tank to the transfer tank until the transfer The liquid level of the liquid in the tank is lower than the transfer high level and higher than the transfer low level.

Optionally, the organ preservation device further includes: a nutrient solution monitoring component, a recovery liquid level monitoring component, a waste liquid tank, and a waste liquid discharge component, wherein a mesh cover is fixedly arranged in the recovery tank, and the mesh cover is used to carry the load. The organ, the nutrient solution monitoring component is located in the transfer tank, the recovery liquid level monitoring component is fixed on the side wall of the recovery tank, and the waste liquid discharge component communicates with the recovery tank and the waste liquid Can;

After the controlling the perfusion assembly is activated, the method further includes:

controlling the recovery liquid level monitoring component to monitor the liquid level of the liquid in the recovery tank;

When the liquid level of the liquid in the recovery tank reaches the recovery high level, adjust the rate at which the return component returns the liquid in the recovery tank to the transfer tank until the liquid level of the liquid in the transfer tank is lower than the required level. The recovery high level;

Controlling the nutrient solution monitoring component to monitor the concentration of a given nutrient substance in the liquid in the transfer tank;

When the concentration of a given nutrient substance in the liquid in the transfer tank is lower than a given concentration, the backflow component is controlled to suspend, and the waste liquid discharge component is controlled to start, so as to discharge the liquid in the recovery tank into the waste tank;

When the liquid level of the liquid in the transfer tank is lower than the transfer low level and the liquid level of the liquid in the recovery tank is lower than the recovery low level, the waste liquid discharge assembly is controlled to suspend, and the height of the recovery high level is equal to The height of the location where the recovery low position is located is all lower than the height of the location where the grille is located;

The liquid replenishing assembly is controlled to start, and when the liquid level of the liquid in the transfer tank reaches the transfer high level, the liquid replenishing assembly is controlled to suspend, and the return flow assembly is controlled to start.

Optionally, the nutrient gas includes oxygen, and the organ preservation device further includes: a liquid-gas analyzer, the liquid-gas analyzer communicates with the gas-liquid output end of the gas-liquid mixing assembly;

The controlling the activation of the gas supply component to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component includes:

During the operation of the air supply component, controlling the air supply component to supply oxygen to the gas-liquid input end of the gas-liquid mixing component;

After the controlling the perfusion assembly is activated, the method further includes:

controlling the liquid-gas analyzer to monitor the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly;

When the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly exceeds a given oxygen partial pressure range, the air-supplying assembly is adjusted to supply oxygen to the gas-liquid input end of the gas-liquid mixing assembly until the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component is restored to within the given oxygen partial pressure range.

Optionally, the nutrient gas includes carbon dioxide;

The controlling the activation of the gas supply component to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component includes:

During the operation of the gas supplement component, controlling the gas supplement component to supplement carbon dioxide to the gas-liquid input end of the gas-liquid mixing component;

After the controlling the perfusion assembly is activated, the method further includes:

Controlling the liquid-gas analyzer to monitor the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly;

When the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component exceeds a given pH range, adjust the rate of supplying carbon dioxide from the gas supply component to the gas-liquid input end of the gas-liquid mixing component until The pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly is restored to the given pH range.

The beneficial effect brought by the technical solution provided by the present application is: in the organ preservation device provided by the present application, the controller controls the gas-liquid input of the gas-supplementing component to the gas-liquid mixing component after controlling the fluid-supplying component to supply the nutrient solution to the transfer tank The nutrient gas is supplied to the end so that the liquid in the transfer tank is pumped by the perfusion component into the gas-liquid input end of the gas-liquid mixing component, and a gas-liquid mixture of nutrient solution and nutrient gas is generated at the gas-liquid output end of the gas-liquid mixing component, and The gas and liquid are perfused into the organ, so that the organ can perform physiological activities; and, after the controller controls the perfusion component to perfuse the organ for a period of time, the nutrient solution will be discharged from the vein of the organ, and after the recovery tank collects the nutrient solution discharged from the organ, The controller controls the return component to return the liquid in the recovery tank to the transfer tank, so that the perfusion component can continuously pump the nutrient solution in the transfer tank into the gas-liquid mixing group component, and output the gas-liquid output end of the gas-liquid mixing component. The gas-liquid perfusion into the organ enables the organ to perform physiological activities in a longer time range, which is beneficial to improve the preservation time of the organ.

It is to be understood that the foregoing general description and the following detailed description are exemplary only and do not limit the application.

Description of drawings

In order to illustrate the embodiments of the present invention more clearly, the following briefly introduces the accompanying drawings used in the description of the embodiments. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention, which are of great significance to the art For those of ordinary skill, other drawings can also be obtained from these drawings without any creative effort.

1 is a schematic structural diagram of an organ preservation device according to an embodiment of the present invention;

2 is a schematic structural diagram of another organ preservation device provided by an embodiment of the present invention;

3 is a schematic structural diagram of another organ preservation device provided by an embodiment of the present invention;

4 is a schematic structural diagram of still another organ preservation device provided by an embodiment of the present invention;

5 is a schematic structural diagram of still another organ preservation device provided by an embodiment of the present invention;

6 is a schematic structural diagram of still another organ preservation device provided by an embodiment of the present invention;

7 is a flowchart of an organ preservation method provided by an embodiment of the present invention;

8 is a flowchart of another organ preservation method provided by an embodiment of the present invention;

FIG. 9 is a flowchart of still another organ preservation method provided by an embodiment of the present invention.

The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the application and together with the description serve to explain the principles of the application.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. . Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

FIG. 1 is a schematic diagram of an organ preservation device according to an embodiment of the present invention. As shown in FIG. 1 , the organ preservation device includes: a controller (not shown in FIG. 1 ), a transfer tank 01 , a recovery tank 02 , and a fluid replacement component 03 , air supply component 04 , gas-liquid mixing component 05 , perfusion component 06 , return component 07 and box 08 . Among them, the transfer tank 01 and the recovery tank 02 are both located in the box body 08, the recovery tank 02 is used for placing the organ 09, the fluid replacement component 03 is communicated with the transfer tank 01, and the gas-liquid input end of the gas-liquid mixing component 05 (not shown in FIG. 1 for the time being) (marked) are connected with the air supply assembly 04 and the transfer tank 01, the gas-liquid output end (not shown in FIG. 1 ) of the gas-liquid mixing assembly 05 is communicated with the perfusion assembly 06, and the perfusion assembly 06 is also connected with the arteries of the organ 09 ( 1) is connected, and the return component 07 is connected to the recovery tank 02 and the transfer tank 01.

The controller can control the start-up of the liquid replenishment component 03 to supply nutrient solution to the transfer tank 01; the controller can also control the start-up of the gas replenishment component 04 to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component 05, and control the perfusion component 06 Start to pump the liquid in the transfer tank 01 into the gas-liquid input end of the gas-liquid mixing assembly 05, and perfuse the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly 05 to the arteries of the organ 09; the controller also The backflow component 07 can be controlled to be activated to return the liquid in the recovery tank 02 to the transfer tank 01 .

To sum up, in the organ preservation device provided by the embodiment of the present invention, after the controller controls the liquid replenishment component to replenish the nutrient solution to the transfer tank, the controller controls the air replenishment component to replenish the nutrient gas to the gas-liquid input end of the gas-liquid mixing component, so that After the perfusion component pumps the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing component, a gas-liquid mixture of nutrient solution and nutrient gas is generated at the gas-liquid output end of the gas-liquid mixing component, and the gas-liquid is perfused to the gas-liquid output end of the gas-liquid mixing component. Organ, so that the organ can perform physiological activities; and, after the controller controls the perfusion component to perfuse the organ for a period of time, the nutrient solution will be discharged from the vein of the organ, and after the recovery tank collects the nutrient solution discharged from the organ, the controller controls the return component to The liquid in the recovery tank is returned to the transfer tank, so that the perfusion component can continuously pump the nutrient solution in the transfer tank into the gas-liquid mixing group component, and perfuse the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component to the organ. The organ can perform physiological activities in a longer time range, which is beneficial to improve the preservation time of the organ.

It should be noted that the gas-liquid input end of the gas-liquid mixing component is connected to the gas-liquid output end. After the gas supply component replenishes the nutrient gas to the gas-liquid input end of the gas-liquid mixing The output end is connected, so after the perfusion component is started, the gas-liquid output end of the gas-liquid mixing component will generate negative pressure, so that the nutrient solution in the transfer tank is pumped into the input end of the gas-liquid mixing component, so that the nutrient solution and nutrient gas are The gas-liquid mixing component is mixed (that is, the nutrient gas is dissolved in the nutrient solution), and is output from the output end of the gas-liquid mixing component, so that the perfusion component can perfuse the mixed gas and liquid into the arteries of the organ.

FIG. 2 is a schematic diagram of another organ preservation device provided by an embodiment of the present invention. As shown in FIG. 2 , the organ preservation device may further include: a liquid level monitoring assembly 10 , and the liquid level monitoring assembly 10 is fixed on the side wall of the transfer tank 01 .

The controller (not shown in FIG. 2 ) can control the liquid level monitoring assembly 10 to monitor the liquid level of the liquid in the transfer tank 01 .

After the liquid level of the liquid in the transfer tank 01 reaches the transfer low level S2, the controller can control the gas supplementing assembly 04 and the perfusion assembly 06 to start. In this way, it can be avoided that the perfusion assembly 06 cannot pump the liquid in the transfer tank 01 into the gas-liquid input end of the gas-liquid mixing assembly 05 because the liquid level of the liquid in the transfer tank 01 is too low.

It should be noted that the perfusion rate of the perfusion assembly 06 is lower than the liquid replenishment rate of the liquid replenishment assembly 03 .

When the liquid level of the liquid in the transfer tank 01 reaches the transfer high level S1, the controller can control the liquid replenishing component 03 to suspend. In this way, it can be avoided that the liquid in the transfer tank 01 overflows during the process that the liquid replenishing component 03 replenishes the liquid in the transfer tank 01 .

The controller can also adjust the return component 07 to return the liquid in the recovery tank 02 to the transfer tank 01 when the liquid level of the liquid in the transfer tank 01 reaches the transfer high level S1 or is lower than the transfer low level S2 during the operation of the return component 07. speed, so that the liquid level of the liquid in the transfer tank 01 is lower than the transfer high level S1 and higher than the transfer low level S2.

For example, during the operation of the return component 07, when the liquid level of the liquid level in the transfer tank 01 reaches the transfer high level S1, the controller can control the rate at which the return component 07 returns the liquid in the recovery tank 02 to the transfer tank 01. Adjust down to make the liquid level of the liquid in the transfer tank 01 gradually decrease until the liquid level of the liquid in the transfer tank 01 is lower than the transfer high level S1; when the liquid level of the liquid in the transfer tank 01 is lower than the transfer low level S2, the controller can The rate of returning the liquid in the recovery tank 02 to the transfer tank 01 by the return component 07 is increased, so that the liquid level of the liquid in the transfer tank 01 is gradually increased until the liquid level of the liquid in the transfer tank 01 is higher than the transfer low level S2.

In this way, it can be avoided at the same time that the liquid level in the transfer tank 01 is too high to overflow, and it can also be avoided that the filling component 06 cannot pump the liquid in the transfer tank 01 because the liquid level of the liquid in the transfer tank 01 is too low. The gas-liquid mixing assembly 05 further improves the continuity of supplying the nutrient solution to the gas-liquid mixing assembly 05, thereby further improving the continuity of perfusing the organ 09 by the perfusion assembly 06, which is beneficial to further increase the duration of organ preservation.

Optionally, the transfer liquid level monitoring assembly 10 may include a transfer high level monitoring element 101 and a transfer low level monitoring element 102, wherein the transfer high level monitoring element 101 is attached to the transfer high position of the side wall of the transfer tank 01, and the transfer low level monitoring element 102 It is attached to the lower part of the side wall of the transfer tank 01. The controller can control the transfer high-level monitoring element 101 and the transfer low-level monitoring element 102 to monitor whether there is liquid at their respective locations. When the transfer high-level monitoring element 101 and the transfer low-level monitoring element 102 both monitor that there is liquid at their respective locations, it indicates that the transfer tank 01 has liquid. The liquid level of the liquid reaches the transfer high level S1. When the transfer high level monitoring element 101 and the transfer low level monitoring element 102 both detect that there is no liquid at their respective positions, it means that the liquid level of the liquid in the transfer tank 01 is lower than the transfer low level S2, and the high level monitoring is performed. When the element 101 detects that there is no liquid in its location and the low-level monitoring element 112 detects that there is liquid in its location, it means that the liquid level of the liquid in the transfer tank 01 is between the transfer high level S1 and the transfer low level S2.

Optionally, the transfer high-level monitoring element 101 and the transfer low-level monitoring element 102 can both be externally measured liquid level switches (such as a capacitive liquid level switch with a measuring principle to determine whether the liquid level point is reached by judging the magnitude of the inductive capacitance, or It is an ultrasonic liquid level switch based on the principle of judging the echo signal strength to judge whether it has reached the liquid level point measurement principle).

FIG. 3 is a schematic structural diagram of another organ preservation device provided by an embodiment of the present invention. As shown in FIG. 3 , the organ preservation device may further include: a nutrient solution monitoring component 11 , a recovery liquid level monitoring component 12 , a waste liquid tank 13 and The waste liquid discharge assembly 14, wherein the recovery tank 02 is fixedly provided with a mesh cover W1, the mesh cover W1 is used to carry the organ 09, the nutrient solution monitoring component 11 is located in the transfer tank 01, and the recovery liquid level monitoring component 12 is fixed in the recovery tank On the side wall of 02, the waste liquid discharge assembly 14 communicates with the recovery tank 02 and the waste liquid tank 13.

A controller (not shown in FIG. 3 ) may control the recovery level monitoring assembly 12 to monitor the level of liquid in the recovery tank 02 .

During the operation of the return component 07, when the liquid level of the liquid in the recovery tank 02 reaches the recovery high level S3, the controller can adjust the rate at which the return component 07 returns the liquid in the recovery tank 02 to the transfer tank 01 until the transfer tank 01 The liquid level of the inner liquid is lower than the recovery high level S3.

In this way, it can be avoided that the liquid level in the recovery tank 02 is too high to overflow. It should be noted that, for the specific process of the rate at which the controller adjusts the return flow component 07 to return the liquid in the recovery tank 02 to the transfer tank 01 according to the liquid in the recovery tank 02, you can refer to the above-mentioned controller to adjust the return flow component 07 according to the liquid in the transfer tank 01 with reference to the specific process. The process of returning the liquid in the recovery tank 02 to the rate of the transfer tank 01 will not be repeated in this embodiment of the present invention.

The controller can also control the nutrient solution monitoring component 11 to monitor the concentration of a given nutrient substance in the liquid in the transfer tank 01, and when the concentration of the given nutrient substance in the liquid in the transfer tank 01 is lower than the given concentration, the control return component 07 is suspended, The waste liquid discharge assembly 14 is controlled to be activated to discharge the liquid in the recovery tank 01 into the waste liquid tank 13 .

It should be noted that when the concentration of the given nutrient in the liquid in the transfer tank 01 is lower than the given concentration, it means that the given nutrient in the liquid circulating between the transfer tank 01 and the recovery tank 02 is about to be consumed by the organ 09. After all, given nutrients in the liquid in the transfer tank 01 are to be replenished, in order to facilitate the subsequent replenishment of new nutrient solution in the transfer tank 01 , the liquid in the recovery tank 02 can be discharged into the waste liquid tank 13 first.

The controller can also control the waste liquid discharge assembly when the liquid level of the liquid in the transfer tank 01 is lower than the transfer low level S2 and the liquid level of the liquid in the recovery tank 02 is lower than the recovery low level S4 during the operation of the waste liquid discharge assembly 14. 14 Pause. It should be noted that, in the height direction of the recovery tank 02, the height of the position where the recovery high position S3 is located and the height of the position where the recovery low position S4 is located are both lower than the height of the position where the mesh cover W1 is located.

In addition, during the operation of the waste liquid discharge assembly 14, since the perfusion assembly 06 has not stopped running, the liquid in the transfer tank 01 will continue to be consumed until the liquid level of the liquid in the transfer tank 01 drops below the transfer low level S2, And, when the liquid level of the liquid in the transfer tank 01 is lower than the transfer low level S2 and the liquid level of the liquid in the recovery tank 02 is lower than the recovery low level S4, it means that the liquid in the transfer tank 01 and the liquid in the recovery tank 02 are basically drained clean. , it is convenient to add new nutrient solution to the transfer tank 01 in the next step.

The controller can control the liquid replenishment assembly 03 to start, and when the liquid level of the liquid in the transfer tank 01 reaches the high transfer high level S1, the controller controls the liquid replenishment assembly 03 to suspend, and controls the return flow assembly 07 to start.

In this way, before the given nutrients in the liquid circulating between the transfer tank 01 and the recovery tank 02 are about to be exhausted by the organs 09, most of the liquid in the transfer tank 01 and the recovery tank 02 can be discharged in time and sent to the transfer tank 09. The tank 01 is replenished with new nutrient solution in a timely manner, so as to continuously provide sufficient nutrients to the organ 01, which is beneficial to further improve the preservation time of the organ 09.

Optionally, the recovery liquid level monitoring assembly 12 may include a recovery high level monitoring element 121 and a recovery low level monitoring element 122, the recovery high level monitoring element 121 is attached to the recovery high position of the side wall of the recovery tank 02, and the recovery low level monitoring element 122 is attached At the recovery low level of the side wall of recovery tank 02. It should be noted that the specific process of the controller controlling the recovery high-level monitoring element 121 and the recovery low-level monitoring element 122 can refer to the above-mentioned monitoring process of the controller controlling the transfer high-level monitoring element 101 and the transfer low-level monitoring element 102. The embodiment will not be repeated here.

Optionally, the given nutrient may be glucose, and the given concentration may be any concentration ranging from 3.9 mmol/L to 6.1 mmol/L, such as 3.9 mmol/L, 4.5 mmol/L liters or 5 mmol per liter, etc. Alternatively, the given substance may be potassium ion, and the given concentration may be any concentration in the range of 3.5 mmol/L to 5.5 mmol/L, such as 3.5 mmol/L, 4.0 mmol/L or 4.5 mmol per liter, etc. Alternatively, the given substance can also be sodium ions, and the given concentration can be any concentration in the range of 135 mmol per liter to 155 mmol per liter, such as 135 mmol per liter, 140 mmol per liter or 145 mmol per liter, etc.

Optionally, the nutrient solution monitoring assembly 11 may include: at least one given nutrient monitoring electrode capable of monitoring the concentration of a given nutrient, the at least one nutrient monitoring electrode may include: a glucose monitoring electrode, a potassium ion monitoring electrode and sodium ion monitoring electrodes, etc.

Optionally, the nutrient gas may include oxygen.

FIG. 4 is a schematic structural diagram of another organ preservation device provided by an embodiment of the present invention. As shown in FIG. 4 , the organ preservation device further includes: a liquid-gas analyzer 15 , a gas of the liquid-gas analyzer 15 and the gas-liquid mixing assembly 05 The liquid output is connected.

The controller (not shown in FIG. 4 ) may also control the gas-liquid input end of the gas-liquid mixing assembly 05 of the gas-supplementation module 04 to supply oxygen during the operation of the gas-supplementation module 04 .

The controller can also control the liquid-gas analyzer 15 to monitor the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly 05 .

When the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly 05 exceeds the given oxygen partial pressure range, the rate of supplying oxygen to the gas-liquid input end of the gas-liquid mixing assembly 05 is adjusted or The concentration of the supplemented oxygen is until the gas-liquid oxygen partial pressure output from the gas-liquid output end of the gas-liquid mixing assembly 05 is restored to the given oxygen partial pressure range.

For example, when the gas-liquid oxygen partial pressure output from the gas-liquid output end of the gas-liquid mixing component 05 is higher than a given oxygen partial pressure range, the controller may supply the gas-liquid mixing component 04 with oxygen to the gas-liquid mixing component 05. Adjust the speed or the concentration of the supplied oxygen to reduce the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly 05 until the oxygen partial pressure of the gas-liquid returns to the given oxygen When the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component 05 is lower than the given oxygen partial pressure range, the controller can supply oxygen from the gas-supplying component 04 to the gas-liquid mixing component 05 Increase the rate of oxygen supply or increase the concentration of the supplied oxygen to increase the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly 05 until the gas-liquid oxygen partial pressure returns to a given value within the oxygen partial pressure range.

In this way, the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly 05 can always be kept within the range of the given partial pressure of oxygen or near the given range of partial pressure of oxygen, avoiding the gas-liquid perfusion into the organ 09 The oxygen partial pressure of the organ 09 is too low, which leads to hypoxia of the organ 09 or the oxygen poisoning of the organ due to the high oxygen partial pressure of the gas-liquid perfused into the organ 09, which is beneficial to further improve the preservation time of the organ.

It should be noted that the oxygen partial pressure of a liquid in which oxygen is dissolved refers to the pressure generated by the dissolved oxygen in the liquid. The oxygen partial pressure can reflect the ability of the liquid to carry oxygen, that is, the higher the oxygen partial pressure, the more oxygen carried by the liquid. The stronger the ability of the liquid, the lower the oxygen partial pressure, and the weaker the liquid's ability to carry oxygen.

Alternatively, a given oxygen partial pressure may range from 300 mmHg to 500 mmHg. It should be noted that millimeters of mercury are millimeters of mercury, which refers to the unit that directly expresses the pressure value in millimeters of the height of the mercury column.

Optionally, the nutrient gas may also include carbon dioxide.

Please continue to refer to FIG. 4 , during the operation of the gas supplementing component 04 , the controller may control the gas supplementing component 04 to supplement carbon dioxide to the input end of the gas-liquid mixing component 05 .

The controller can control the gas-liquid 16 analysis to monitor the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly 05 .

When the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component 05 exceeds the given pH range, the controller can adjust the rate of supplying carbon dioxide from the gas supply component 04 to the gas-liquid input end of the gas-liquid mixing component 05 until The pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly is restored to the given pH range.

For example, when the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly 05 is higher than a given pH range, the controller can supply carbon dioxide to the gas-liquid input end of the gas-liquid mixing assembly 05 from the air supply assembly 04 . The speed of the nutrient solution is increased, so that the concentration of carbon dioxide dissolved in the nutrient solution is gradually increased, so that the acidity of the nutrient solution dissolved in carbon dioxide (that is, the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component 05) is gradually increased. , the pH gradually decreases until the pH of the gas-liquid returns to the given pH range. When the pH of the nutrient solution in which carbon dioxide is dissolved is lower than a given pH range, the controller can adjust the rate of supplying carbon dioxide from the supply component 05 to the gas-liquid input end of the gas-liquid mixing component 05 to reduce the rate of carbon dioxide dissolved in the nutrient solution. The concentration of carbon dioxide gradually decreases, so that the alkalinity of the nutrient solution dissolved in carbon dioxide (that is, the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component 05) gradually increases, and the pH gradually increases until the pH of the gas-liquid recovers. to a given pH range.

It should be noted that, in the embodiment of the present invention, the pH of the gas-liquid refers to the hydrogen ion concentration index (that is, the pH value). The higher the pH, the stronger the alkalinity and the weaker the acidity. The stronger, the weaker the alkalinity.

It should be noted that, in the process of perfusing the gas-liquid output from the gas-liquid output end of the gas-liquid mixing module 05 into the arteries of the organ 09, part of the gas-liquid is brought into the liquid-gas analyzer 15 at the same time, so that The oxygen partial pressure and pH of the gas and liquid were analyzed in the subsequent liquid gas analyzer.

Optionally, in the process of controlling the liquid-gas analyzer to monitor the oxygen partial pressure and pH of the gas and liquid, the controller may control the liquid-gas analyzer to analyze the oxygen partial pressure and pH of the gas and liquid once every given time period. For example, the given duration may be any duration ranging from 30 minutes to 40 minutes, such as 30 minutes, 33 minutes, 35 minutes, or 37 minutes.

FIG. 5 is a schematic structural diagram of another organ preservation device provided by an embodiment of the present invention. As shown in FIG. 5 , the box body may include: an outer box 081 , an inner box 082 and a heating element 083 , and the outer box 081 wraps the inner box 082 , the heating element 083 is located between the outer box 081 and the inner box 082 , the inner box 082 is provided with a water bath 16 and a temperature measuring element 17 , and the transfer tank 01 and the recovery tank 02 are placed in the water bath 16 .

The controller can control the temperature measuring element 17 to monitor the temperature in the water bath 16, and after the temperature in the water bath 16 exceeds a given temperature range, adjust the heating power of the heating element 083 to restore the temperature in the water bath 16 to a given temperature temperature range.

For example, when the temperature measuring element 17 detects that the temperature in the water bath 16 is higher than a given temperature range, the controller can reduce the heating power of the heating element 083 to gradually reduce the temperature in the water bath 16 until the water bath The temperature in 16 is restored to a given temperature range; when the temperature measuring element 17 monitors that the temperature in the water bath 16 is lower than the given temperature range, the controller can increase the heating power of the heating element 083 to make the temperature in the water bath 16 increase. The temperature gradually increases until the temperature in the water bath 16 returns to a given temperature range.

Under such a structure, on the one hand, because the water bath 16 is a liquid medium, the transfer tank 01 and the recovery tank 02 are in contact with the liquid medium, and because the fluidity of the liquid medium is strong, the heating of the transfer tank 01 and the recovery tank 02 is relatively uniform. , the temperature change process in the transfer tank 01 and the recovery tank 02 is relatively uniform, the temperature change of the liquid perfused to the organ 09 is relatively uniform, and the stability of the physiological environment of the organ 09 is higher, which is conducive to further improving the preservation time of the organ 09; On the one hand, since the box body is a double-layer box body structure, and the heating element is located between the inner and outer box bodies, the temperature is not easily lost, and the insulation effect of the box body is better.

Alternatively, the given temperature range may be between 36.5 degrees Celsius and 37.5 degrees Celsius.

In this way, the organ can be preserved under the normal physiological temperature of the human body, and the preservation time of the organ can be further improved.

Optionally, the box body may include a plurality of heating elements 083 , and the plurality of heating elements 083 may be attached to the box wall of the inner box 082 . For example, the box body may include three heating elements 083, and the three heating elements 083 may be distributed on the bottom box wall and the two side box walls of the inner box 082, respectively.

FIG. 6 is a schematic diagram of still another organ preservation device provided by an embodiment of the present invention. As shown in FIG. 6 , the fluid replacement component may include: a first peristaltic pump 031 , a nutrient solution source 032 , a first suction tube 033 and a fluid replacement tube 034 , wherein the first peristaltic pump 031 communicates with the nutrient solution source 032 through the first liquid suction pipe 033 , and communicates with the transfer tank 01 through the liquid replenishment pipe 034 .

The controller (not shown in FIG. 6 ) can control the start of the first peristaltic pump 031 to pump the nutrient solution in the nutrient solution source 032 into the transfer tank 01 in the process of controlling the replenishment component to replenish the nutrient solution in the transfer tank 01. , and when the liquid level of the liquid in the transfer tank 01 reaches the transfer high level S1, the first peristaltic pump 031 can be controlled to pause, so as to control the liquid replenishment component to pause.

Alternatively, the nutrient solution in the nutrient solution source 032 can be a cell culture medium, such as 1640/DMEM culture medium or DMEM culture medium, it should be noted that 1640/DMEM culture medium and DMEM culture medium are mature in related technologies. Applied cell culture medium.

Optionally, the air supply assembly may include: an air pump 041, a first flow valve 042, a second flow valve 043, a third flow valve 044, a first air extraction pipe 045, a second air extraction pipe 046, a third air extraction pipe 047, an inlet A gas pipe 048, a supplemental gas pipe 049, an oxygen source N1 and a carbon dioxide source N2. Wherein, the air pump 041 communicates with the first flow valve 042, the second flow valve 043 and the third flow valve 053 through the intake pipe 048, and communicates with the gas-liquid input end of the gas-liquid mixing assembly 05 through the air supply pipe 058; the first flow valve 042 is connected to the oxygen source N1 through the first exhaust pipe 045, the second flow valve 043 is connected to the outside air through the second exhaust pipe 046, and the third flow valve 044 is connected to the carbon dioxide source N2 through the third exhaust pipe 047.

It should be noted that the oxygen source N1 stores a high concentration of oxygen, and the concentration of the high concentration oxygen is much higher than the concentration of oxygen in the air; the carbon dioxide source N2 stores a high concentration of carbon dioxide, and the concentration of the high concentration of carbon dioxide is much higher than that of the air. concentration of carbon dioxide.

In the process of controlling the air supply component to supply oxygen to the gas-liquid input end of the gas-liquid mixing component 05, the controller can control the first flow valve 042 and/or the second flow valve 043 to open, and control the air pump 041 to connect the oxygen source N1 and /or oxygen in the outside air is pumped to the gas-liquid input end of the gas-liquid mixing assembly 05 .

In addition, the controller can adjust the rate of supplying oxygen to the gas-liquid input end of the gas-liquid mixing assembly 05 by adjusting the flow rate of the first flow valve 042 and/or the second flow valve 043 . The controller may adjust the flow rate difference between the flow rate of the first flow valve 042 and the flow rate of the second flow valve 043 to adjust the concentration of oxygen supplied to the gas-liquid input end of the gas-liquid mixing assembly 05; for example, the controller may The flow rate of the first flow valve 042 is adjusted up, and the flow rate of the second flow valve 043 is adjusted down, so that the concentration of oxygen supplied by the air pump 041 to the gas-liquid mixing assembly 05 is increased. In addition, the controller can also increase the first flow rate The flow rate of the valve 042 is adjusted down, and the flow rate of the second flow valve 043 is adjusted up, so that the concentration of oxygen supplied by the air pump 041 to the gas-liquid mixing assembly 05 is reduced.

In the process of controlling the gas supply component to supply carbon dioxide to the gas-liquid input end of the gas-liquid mixing component 05, the controller can control the third flow valve 044 to open, and control the air pump 041 to pump the carbon dioxide in the carbon dioxide source X2 to the gas-liquid mixing component. 05's gas-liquid input. In addition, the controller can also adjust the rate of supplying carbon dioxide to the gas-liquid input end of the gas-liquid mixing assembly 05 by adjusting the flow rate of the third flow valve 044 .

Optionally, the organ preservation device may further include: an infusion tube 18 , and the gas-liquid input end of the gas-liquid mixing assembly 05 is communicated with the transfer tank 01 through the infusion tube 18 .

Optionally, the perfusion assembly may include: a second peristaltic pump 061, a second liquid suction pipe 062 and a perfusion pipe 063, wherein the second peristaltic pump 061 and the gas-liquid output of the gas-liquid mixing assembly 05 through the second liquid suction pipe 062 The second peristaltic pump 061 communicates with the arteries of the organ 09 through the perfusion tube 063.

The controller can control the second peristaltic pump 061 to pump the liquid in the transfer tank 01 into the gas-liquid input end of the gas-liquid mixing assembly 05, and perfuse the liquid output from the gas-liquid output end of the gas-liquid mixing assembly 05 to the organ 09. artery.

Optionally, the return component may include: a third peristaltic pump 071, a third suction pipe 072, and a return pipe 073, wherein the third peristaltic pump 071 may communicate with the recovery tank 02 through the third suction pipe 072, and pass through The liquid return pipe 073 communicates with the transfer tank 01 .

Optionally, the third liquid suction pipe 072 communicates with the recovery tank 02 at the bottom of the recovery tank 02 , and the liquid return pipe 073 communicates with the transfer tank 01 at the bottom of the transfer tank 01 .

In the process of controlling the operation of the backflow component, the controller can control the third peristaltic pump 071 to pump the liquid collected in the recovery tank 02 into the transfer tank 01. In addition, the liquid level of the liquid in the transfer tank 01 reaches the transfer high level S1 or low. During the transfer low level S2, the speed of the liquid collected in the recovery tank 02 can be pumped into the transfer tank 01 by adjusting the third peristaltic pump 071, so that the liquid level of the liquid in the transfer tank 01 is lower than the transfer high level S1 and higher than the transfer low level. S2, and when the nutrient solution monitoring component 11 monitors that the concentration of the given nutrient in the liquid in the transfer tank 01 is lower than the given concentration, the third peristaltic pump 071 can be controlled to suspend.

Optionally, the waste liquid discharge assembly may include: a fourth peristaltic pump 141, a fourth suction pipe 142 and a discharge pipe 143, wherein the fourth peristaltic pump 141 communicates with the recovery tank 02 through the fourth suction pipe 142, and It communicates with the waste liquid tank 13 through the drain pipe 143 .

Optionally, the fourth liquid suction pipe 142 communicates with the recovery tank 02 at the bottom of the recovery tank 02 .

In the process of controlling the operation of the waste liquid discharge assembly, the controller can control the fourth peristaltic pump 141 to discharge the liquid in the recovery tank 02 into the waste liquid tank 13, and the liquid level of the liquid in the transfer tank 01 is lower than the transfer low level S2. And when the liquid level of the liquid in the recovery tank 02 is lower than the recovery low level S4, the fourth peristaltic pump 141 is controlled to pause.

Optionally, the organ preservation device may further include: a liquid outlet pipe 19 , and the gas-liquid output end of the gas-liquid mixing assembly 05 may be communicated with the liquid-gas analyzer 15 through the liquid outlet pipe 19 .

Optionally, the organ preservation device may further include a buzzer (not shown in Figure 6). During the operation of the perfusion assembly, when the liquid level in the transfer tank 01 is higher than the transfer high level S1 or lower than the transfer low level S2, and the liquid level in the recovery tank 02 is higher than the recovery high level S3 or lower than the recovery low level S4 , or when the temperature in the water bath 16 exceeds a given temperature range, the controller can control the buzzer to give an alarm to remind the staff and avoid accidents.

Optionally, the gas-liquid mixing assembly 05 may be a membrane lung. It should be noted that membrane lung, also known as extracorporeal membrane oxygenation, can play the role of artificial heart and lung, and can dissolve nutrient gases such as oxygen and carbon dioxide into the nutrient solution.

Optionally, in the height direction of the transfer tank 01, the distance from the transfer high position S1 to the tank mouth of the transfer tank 02, and the distance from the transfer low position S2 to the nozzle of the infusion tube 18 can be the first given distance, and the transfer high position The height of S1 is lower than the mouth of the transfer tank 01 , and the height of the low transfer position S2 is higher than the mouth of the infusion pipe 18 . Optionally, the first given distance may be any length between 3 mm and 10 mm, for example, the first given distance may be: 3 mm, 5 mm, 7 mm, 9 mm or 10 mm.

Optionally, in the height direction of the recovery tank 02, the distance between the recovery high position S3 and the mesh cover W1, and the distance from the recovery low position S4 to the fourth suction pipe 142 can be the second given distance, and the recovery low position S4 The height is higher than the mouth of the fourth suction pipe 142 . Optionally, the second predetermined distance may be any length between 3 mm and 10 mm, for example, the second predetermined distance may be: 3 mm, 5 mm, 7 mm, 9 mm or 10 mm.

It should be noted that in FIGS. 1 to 6 , it is only taken as an example that each component in the organ preservation device is independent of the box body. The peristaltic pump in the perfusion assembly and the waste liquid discharge assembly may be integrated into the inner wall of the box, and the pipe connected with the peristaltic pump may extend into the inner wall of the box, which is not limited in this embodiment of the present invention.

Optionally, the organ 09 may be an internal organ such as liver, spleen, lung, kidney, heart or small intestine in the human body.

It should be noted that, in the embodiment of the present invention, the controller of the organ preservation device can communicate with each component (each peristaltic pump, each flow valve, air pump, each liquid level monitoring element, each heating element, Temperature measuring element, liquid gas analyzer, nutrient solution monitoring component) are connected and communicated. Alternatively, the controller may also communicate in other manners (eg, wireless communication), which is not limited in this embodiment of the present invention.

To sum up, in the organ preservation device provided by the embodiment of the present invention, after the controller controls the liquid replenishment component to replenish the nutrient solution to the transfer tank, the controller controls the air replenishment component to replenish the nutrient gas to the gas-liquid input end of the gas-liquid mixing component, so that After the perfusion component pumps the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing component, a gas-liquid mixture of nutrient solution and nutrient gas is generated at the gas-liquid output end of the gas-liquid mixing component, and the gas-liquid is perfused to the gas-liquid output end of the gas-liquid mixing component. Organ, so that the organ can perform physiological activities; and, after the controller controls the perfusion component to perfuse the organ for a period of time, the nutrient solution will be discharged from the vein of the organ, and after the recovery tank collects the nutrient solution discharged from the organ, the controller controls the return component to The liquid in the recovery tank is returned to the transfer tank, so that the perfusion component can continuously pump the nutrient solution in the transfer tank into the gas-liquid mixing group component, and perfuse the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component to the organ. The organ can perform physiological activities in a longer time range, which is beneficial to improve the preservation time of the organ.

7 is a flowchart of an organ preservation method provided in an embodiment of the present invention. The organ preservation method may be used in a controller in any of the above-mentioned organ preservation devices, and the organ preservation method may include:

Step 701: Control the activation of the liquid replenishing component to replenish the nutrient solution to the transfer tank.

Step 702: Control the activation of the gas supply component to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component.

Step 703: Control the start of the perfusion component to pump the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing component, and perfuse the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component to the arteries of the organ.

Step 704: Control the activation of the return component to return the liquid in the recovery tank to the transfer tank.

To sum up, in the organ preservation method provided by the embodiment of the present invention, after the fluid replenishing component is controlled to supply nutrient solution to the transfer tank, the air replenishing component is controlled to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component, so as to facilitate perfusion. After the component pumps the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing component, the gas-liquid mixing nutrient solution and nutrient gas is generated at the gas-liquid output end of the gas-liquid mixing component, and the gas-liquid is perfused to the organ, The organ can perform physiological activities; and, after controlling the perfusion component to perfuse the organ for a period of time, the nutrient solution will be discharged from the vein of the organ. The liquid is returned to the transfer tank, so that the perfusion component can continuously pump the nutrient solution in the transfer tank into the gas-liquid mixing group component, and perfuse the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component to the organ, so that the organ can be Physiological activities are carried out in a longer time frame, which is beneficial to improve the time of organ preservation.

8 is a flowchart of another organ preservation method provided by an embodiment of the present invention. The organ preservation method can be used in a controller in the above-mentioned organ preservation device including a transfer liquid level monitoring component, and the organ preservation method can include:

Step 801: Control the activation of the liquid replenishing component to replenish the nutrient solution to the transfer tank.

Step 802 , controlling the transfer liquid level monitoring component to monitor the liquid level of the liquid in the transfer tank.

Step 803 , after the liquid level of the liquid in the transfer tank reaches the transfer low level, control the air supply component and the perfusion component to start.

It should be noted that the perfusion rate of the perfusion assembly is lower than the fluid replacement rate of the fluid replacement component.

Step 804: When the liquid level of the liquid in the transfer tank reaches the transfer high level, control the liquid replenishing component to suspend.

Step 805: Control the activation of the return component to return the liquid in the recovery tank to the transfer tank.

Step 806, when the liquid level of the liquid in the transfer tank reaches the transfer high level or is lower than the transfer low level, adjust the rate at which the return component returns the liquid in the recovery tank to the transfer tank until the liquid level of the liquid in the transfer tank is lower than the transfer high level and above the interim low.

9 is a flowchart of another organ preservation method provided by an embodiment of the present invention. The organ preservation method can be used for the above-mentioned components including a nutrient solution monitoring component, a recovery liquid level monitoring component, a waste liquid tank, and a waste liquid discharge component, and The controller is fixedly arranged in the organ preservation device of the mesh cover in the recovery tank, and the organ preservation method may include:

Step 901: Control the activation of the liquid replenishing component to replenish the nutrient solution to the transfer tank.

Step 902 , controlling the transfer liquid level monitoring component to monitor the liquid level of the liquid in the transfer tank.

Step 903 , after the liquid level of the liquid in the transfer tank reaches the transfer low level, control the air supply component and the perfusion component to start.

It should be noted that the perfusion rate of the perfusion assembly is lower than the fluid replacement rate of the fluid replacement component.

Step 904: When the liquid level of the liquid in the transfer tank reaches the transfer high level, control the liquid replenishing component to suspend.

Step 905 , control the activation of the return component to return the liquid in the recovery tank to the transfer tank.

Step 906, when the liquid level of the liquid in the transfer tank reaches the transfer high level or is lower than the transfer low level, adjust the rate at which the return component returns the liquid in the recovery tank to the transfer tank until the liquid level of the liquid in the transfer tank is lower than the transfer high level and above the interim low.

Step 907 , controlling the recovery liquid level monitoring component to monitor the liquid level of the liquid in the recovery tank.

Step 908: When the liquid level of the liquid in the recovery tank reaches the recovery high level, adjust the rate at which the return component returns the liquid in the recovery tank to the transfer tank until the liquid level of the liquid in the transfer tank is lower than the recovery high level.

Step 909 , controlling the nutrient solution monitoring component to monitor the concentration of a given nutrient substance in the liquid in the transfer tank.

Step 910: When the concentration of the given nutrient in the liquid in the transfer tank is lower than the given concentration, control the backflow component to suspend, and control the waste liquid discharge component to start, so as to discharge the liquid in the recovery tank into the waste liquid tank.

Step 911 , when the liquid level of the liquid in the transfer tank is lower than the transfer low level and the liquid level of the liquid in the recovery tank is lower than the recovery low level, control the waste liquid discharge component to suspend.

It should be noted that the height of the position where the recovery high position is located and the height of the position where the recovery low position is located are both lower than the height of the position where the grille is located.

Step 912 , control the liquid replenishing component to start, and when the liquid level of the liquid in the transfer tank reaches the high level of the transfer, control the liquid replenishing component to suspend, and control the return component to start.

Optionally, in any of the above steps 703, 803 and 903, the controller in the organ preservation device may control the air supply component to supply oxygen to the gas-liquid input end of the gas-liquid mixing component.

Optionally, in any of the above organ preservation methods, the controller can control the liquid-gas analyzer to monitor the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly; When the oxygen partial pressure of the gas-liquid output from the gas-liquid terminal exceeds the given oxygen partial pressure range, adjust the oxygen supply rate or the oxygen concentration of the supplied oxygen from the gas-liquid mixing component to the gas-liquid input end of the gas-liquid mixing component until the gas-liquid mixing component The gas-liquid oxygen partial pressure output from the gas-liquid output terminal is restored to the given oxygen partial pressure range.

Optionally, in any one of the above steps 703, 803 and 903, the controller in the organ preservation device may control the gas supply component to supply carbon dioxide to the gas-liquid input end of the gas-liquid mixing component.

Optionally, in any of the above organ preservation methods, the controller can control the liquid-gas analyzer to monitor the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly; When the pH of the output gas-liquid exceeds the given pH range, adjust the rate of supplying carbon dioxide from the gas supply component to the gas-liquid input end of the gas-liquid mixing component until the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component recovers up to the given pH range.

Optionally, in the process of controlling the liquid-gas analyzer to monitor the oxygen partial pressure and pH of the gas and liquid, the controller may control the liquid-gas analyzer to analyze the oxygen partial pressure and pH of the gas and liquid once every given time period.

Optionally, in any of the above organ preservation methods, when the organ preservation device includes a water bath, a temperature measuring element and a heating element, the organ preservation method may further include: controlling the temperature measuring element to monitor the temperature in the water bath, After the temperature in the water bath exceeds the given temperature range, the heating power of the heating element is adjusted to restore the temperature in the water bath to the given temperature range.

Optionally, when the fluid replacement component in the organ preservation device includes the first peristaltic pump, the nutrient solution source, the first fluid suction tube and the fluid replacement tube, in any of the above steps 701, 801, 901 and 912 In the above step 804 or step 904, when the liquid level of the liquid in the transfer tank reaches the transfer high level, the controller can Control the first peristaltic pump to pause.

Optionally, the air supply component in the organ preservation device includes an air pump, a first flow valve, a second flow valve, a third flow valve, a first air suction pipe, a second air suction pipe, a third In the case of a trachea, an oxygen source and a carbon dioxide source, in any of the above steps 702, 803 and 903, the controller can control the first flow valve and/or the second flow valve to open, and control the air pump. / or the oxygen in the oxygen source is pumped to the gas-liquid input end of the gas-liquid mixing component; in addition, the controller can control the third flow valve to open, and control the air pump to pump carbon dioxide from the carbon dioxide source to the gas-liquid input of the gas-liquid mixing component end. In addition, the controller can adjust the rate of supplying air to the gas-liquid input end of the gas-liquid mixing assembly by adjusting the flow rate of the first flow valve and/or the flow rate of the second flow valve, and can adjust the flow rate of the third flow valve to adjust the air supply. Regulates the rate at which carbon dioxide is supplied to the gas-liquid input of the gas-liquid mixing assembly.

Optionally, when the perfusion assembly in the organ preservation device includes: a second peristaltic pump, a second suction tube and a perfusion tube, in any of the above steps 703, 803 and 903, the controller may control the The second peristaltic pump pumps the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing assembly.

Optionally, when the return component in the organ preservation device includes the third peristaltic pump, the third suction pipe and the return pipe, in any of the above steps 704, 805 and 905, the controller can control the The third peristaltic pump pumps the liquid collected in the recovery tank into the transfer tank. In the above step 806 or step 908, the controller can adjust the rate of the third peristaltic pump to pump the liquid collected in the recovery into the transfer, so that the liquid level of the liquid in the transfer tank is lower than the transfer high level and higher than the transfer low level. In the above step 910, when the nutrient solution monitoring component detects that the concentration of the given nutrient in the liquid in the transfer tank is lower than the given concentration, the third peristaltic pump is controlled to suspend.

Optionally, when the waste liquid discharge assembly in the organ preservation device includes the fourth peristaltic pump, the fourth suction pipe and the drain pipe, in the above-mentioned step 910, the controller can control the fourth peristaltic pump to recover the waste in the tank. The liquid is discharged into the waste liquid tank; in the above step 911, when the liquid level of the liquid in the transfer tank is lower than the transfer low level and the liquid level of the liquid in the recovery tank is lower than the recovery low level, the fourth peristaltic pump is controlled to pause.

To sum up, in the organ preservation method provided by the embodiment of the present invention, after the fluid replenishing component is controlled to supply nutrient solution to the transfer tank, the air replenishing component is controlled to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component, so as to facilitate perfusion. After the component pumps the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing component, the gas-liquid mixing nutrient solution and nutrient gas is generated at the gas-liquid output end of the gas-liquid mixing component, and the gas-liquid is perfused to the organ, The organ can perform physiological activities; and, after controlling the perfusion component to perfuse the organ for a period of time, the nutrient solution will be discharged from the vein of the organ. The liquid is returned to the transfer tank, so that the perfusion component can continuously pump the nutrient solution in the transfer tank into the gas-liquid mixing group component, and perfuse the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component to the organ, so that the organ can be Physiological activities are carried out in a longer time frame, which is beneficial to improve the time of organ preservation.

It should be noted that, the apparatus embodiments provided in the embodiments of the present invention can refer to each other with the corresponding method embodiments, which are not limited in the embodiments of the present invention. The sequence of the steps of the method embodiments provided by the embodiments of the present invention can be appropriately adjusted, and the steps can also be increased or decreased according to the situation. All methods should be covered within the protection scope of the present invention, and therefore will not be repeated here.

Other embodiments of the invention will readily occur to those skilled in the art upon consideration of the specification and practice of the invention disclosed herein. This application is intended to cover any variations, uses or adaptations of the invention which follow the general principles of the invention and which include common knowledge or conventional techniques in the art to which the invention is not invented . The specification and examples are to be regarded as exemplary only, with the true scope and spirit of the invention being indicated by the claims.

It should be understood that the present invention is not limited to the precise structures described above and illustrated in the accompanying drawings, and that various modifications and changes may be made without departing from its scope. The scope of the present invention is limited only by the appended claims.

Claims (10)

1. An organ preservation device, characterized in that, the organ preservation device comprises: a controller, a transfer tank, a recovery tank, a liquid replenishing assembly, an air replenishing assembly, a gas-liquid mixing assembly, a perfusion assembly, a return flow assembly and a casing; The transfer tank and the recovery tank are both located in the box, the recovery tank is used for placing organs, the fluid replacement component is communicated with the transfer tank, and the gas-liquid input end of the gas-liquid mixing component is connected to the The gas supply component and the transfer tank are both connected, the gas-liquid output end of the gas-liquid mixing component is connected with the perfusion component, the perfusion component is also connected with the artery of the organ, and the return component is connected with the recovery component tank and the transfer tank; the controller is used for: controlling the start of the liquid replenishing component to supply nutrient solution to the transfer tank; controlling the start of the gas supply component to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component; Control the perfusion component to start, to pump the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing component, and perfuse the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component to the arteries of said organ; Control the activation of the return component to return the liquid in the recovery tank to the transfer tank. 2 . The organ preservation device according to claim 1 , wherein the organ preservation device further comprises: a transfer liquid level monitoring component, the transfer liquid level monitoring component being fixed on the side wall of the transfer tank, and the Controllers are also used to: controlling the transfer liquid level monitoring component to monitor the liquid level of the liquid in the transfer tank; After the liquid level of the liquid in the transfer tank reaches the transfer low level, controlling the gas supplementation component and the perfusion component to start, and the perfusion rate of the perfusion component is less than the liquid supplementation rate of the liquid supplementation component; When the liquid level of the liquid in the transfer tank reaches the transfer high level, the liquid replenishing component is controlled to suspend; During the operation of the return component, when the liquid level of the liquid in the transfer tank reaches the high transfer level or is lower than the low transfer level, the return component is adjusted to return the liquid in the recovery tank to the desired level. The speed of the transfer tank is increased until the liquid level of the liquid in the transfer tank is lower than the transfer high level and higher than the transfer low level. 3 . The organ preservation device according to claim 2 , wherein the organ preservation device further comprises: a nutrient solution monitoring component, a recovery liquid level monitoring component, a waste liquid tank and a waste liquid discharge component, wherein the recovery tank A mesh cover is fixedly arranged, the mesh cover is used to carry the organ, the nutrient solution monitoring component is located in the transfer tank, the recovery liquid level monitoring component is fixed on the side wall of the recovery tank, and the waste The liquid discharge assembly communicates with the recovery tank and the waste liquid tank, and the controller is also used for: controlling the recovery liquid level monitoring component to monitor the liquid level of the liquid in the recovery tank; During the operation of the return component, when the liquid level of the liquid in the recovery tank reaches a high recovery level, adjust the rate at which the return component returns the liquid in the recovery tank to the transfer tank until the The liquid level of the liquid in the transfer tank is lower than the recovery high level; Controlling the nutrient solution monitoring component to monitor the concentration of a given nutrient substance in the liquid in the transfer tank; When the concentration of a given nutrient substance in the liquid in the transfer tank is lower than a given concentration, the backflow component is controlled to suspend, and the waste liquid discharge component is controlled to start, so as to discharge the liquid in the recovery tank into the waste tank; During the operation of the waste liquid discharge assembly, when the liquid level of the liquid in the transfer tank is lower than the transfer low level and the liquid level of the liquid in the recovery tank is lower than the recovery low level, the waste liquid is controlled to discharge The assembly is suspended, and the height of the position of the recovery high position and the height of the position of the recovery low position are both lower than the height of the position of the grille; The liquid replenishing assembly is controlled to start, and when the liquid level of the liquid in the transfer tank reaches the transfer high level, the liquid replenishing assembly is controlled to suspend, and the return flow assembly is controlled to start. 4. The organ preservation device according to any one of claims 1 to 3, wherein the nutrient gas comprises oxygen, and the organ preservation device further comprises: a liquid-gas analyzer, the liquid-gas analyzer and the The gas-liquid output end of the gas-liquid mixing assembly is connected, and the controller is also used for: During the operation of the air supply component, controlling the air supply component to supply oxygen to the gas-liquid input end of the gas-liquid mixing component; controlling the liquid-gas analyzer to monitor the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly; When the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly exceeds a given oxygen partial pressure range, the air-supplying assembly is adjusted to supply oxygen to the gas-liquid input end of the gas-liquid mixing assembly until the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component is restored to within the given oxygen partial pressure range. 5. The organ preservation device according to claim 4, wherein the nutrient gas comprises carbon dioxide, and the controller is further used for: During the operation of the gas supplement component, controlling the gas supplement component to supplement carbon dioxide to the gas-liquid input end of the gas-liquid mixing component; Controlling the liquid-gas analyzer to monitor the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly; When the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component exceeds a given pH range, adjust the rate of supplying carbon dioxide from the gas supply component to the gas-liquid input end of the gas-liquid mixing component until The pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component is restored to the given pH range. 6. An organ preservation method, wherein the organ preservation method is used in a controller in the organ preservation device according to any one of claims 1 to 5, and the organ preservation device further comprises: a transfer tank, a recovery tank , liquid replenishment components, air replenishment components, gas-liquid mixing components, perfusion components, return components and cabinets; The transfer tank and the recovery tank are both located in the box, the recovery tank is used for placing organs, the fluid replacement assembly is communicated with the transfer tank, and the gas-liquid input end of the gas-liquid mixing assembly is connected to the The gas supply component and the transfer tank are both connected, the gas-liquid output end of the gas-liquid mixing component is connected with the perfusion component, the perfusion component is also connected with the artery of the organ, and the return component is connected with the recovery component tank and the transfer tank; the method includes: controlling the start of the liquid replenishing component to supply nutrient solution to the transfer tank; controlling the start of the gas supply component to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component; Control the perfusion component to start, to pump the liquid in the transfer tank into the gas-liquid input end of the gas-liquid mixing component, and perfuse the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component to the arteries of said organ; Control the activation of the return component to return the liquid in the recovery tank to the transfer tank. 7 . The organ preservation method according to claim 6 , wherein the organ preservation device further comprises: a transfer liquid level monitoring assembly, the transfer liquid level monitoring assembly being fixed on the side wall of the transfer tank; 7 . After the control of the rehydration component is started, the method further includes: controlling the transfer liquid level monitoring component to monitor the liquid level of the liquid in the transfer tank; After the liquid level of the liquid in the transfer tank reaches the transfer low level, controlling the gas supplementation component and the perfusion component to start, and the perfusion rate of the perfusion component is less than the liquid supplementation rate of the liquid supplementation component; When the liquid level of the liquid in the transfer tank reaches the transfer high level, the liquid replenishing component is controlled to suspend; After the control of the reflow assembly is started, the method further includes: When the liquid level of the liquid in the transfer tank reaches the transfer high level or is lower than the transfer low level, adjust the rate at which the return component returns the liquid in the recovery tank to the transfer tank until the transfer The liquid level of the liquid in the tank is lower than the transfer high level and higher than the transfer low level. 8 . The organ preservation method according to claim 7 , wherein the organ preservation device further comprises: a nutrient solution monitoring component, a recovery liquid level monitoring component, a waste liquid tank and a waste liquid discharge component. A mesh cover is fixedly arranged, the mesh cover is used to carry the organ, the nutrient solution monitoring component is located in the transfer tank, the recovery liquid level monitoring component is fixed on the side wall of the recovery tank, and the waste The liquid discharge assembly communicates with the recovery tank and the waste liquid tank; After the controlling the perfusion assembly is activated, the method further includes: controlling the recovery liquid level monitoring component to monitor the liquid level of the liquid in the recovery tank; When the liquid level of the liquid in the recovery tank reaches the recovery high level, adjust the rate at which the return component returns the liquid in the recovery tank to the transfer tank until the liquid level of the liquid in the transfer tank is lower than the required level. The recovery high level; Controlling the nutrient solution monitoring component to monitor the concentration of a given nutrient substance in the liquid in the transfer tank; When the concentration of a given nutrient substance in the liquid in the transfer tank is lower than a given concentration, the backflow component is controlled to suspend, and the waste liquid discharge component is controlled to start, so as to discharge the liquid in the recovery tank into the waste tank; When the liquid level of the liquid in the transfer tank is lower than the transfer low level and the liquid level of the liquid in the recovery tank is lower than the recovery low level, the waste liquid discharge assembly is controlled to suspend, and the height of the recovery high level is equal to The height of the location where the recovery low position is located is all lower than the height of the location where the grille is located; The liquid replenishing assembly is controlled to start, and when the liquid level of the liquid in the transfer tank reaches the transfer high level, the liquid replenishing assembly is controlled to suspend, and the return flow assembly is controlled to start. 9. The organ preservation method according to any one of claims 6 to 8, wherein the nutrient gas comprises oxygen, and the organ preservation device further comprises: a liquid-gas analyzer, the liquid-gas analyzer and the The gas-liquid output end of the gas-liquid mixing component is connected; The controlling the activation of the gas supply component to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component includes: During the operation of the air supply component, controlling the air supply component to supply oxygen to the gas-liquid input end of the gas-liquid mixing component; After the controlling the perfusion assembly is activated, the method further includes: controlling the liquid-gas analyzer to monitor the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly; When the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly exceeds a given oxygen partial pressure range, the air-supplying assembly is adjusted to supply oxygen to the gas-liquid input end of the gas-liquid mixing assembly until the oxygen partial pressure of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly is restored to within the given oxygen partial pressure range. 10. The organ preservation method according to claim 9, wherein the nutrient gas comprises carbon dioxide; The controlling the activation of the gas supply component to supply nutrient gas to the gas-liquid input end of the gas-liquid mixing component includes: During the operation of the gas supplement component, controlling the gas supplement component to supplement carbon dioxide to the gas-liquid input end of the gas-liquid mixing component; After the controlling the perfusion assembly is activated, the method further includes: Controlling the liquid-gas analyzer to monitor the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly; When the pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing component exceeds a given pH range, adjust the rate of supplying carbon dioxide from the gas supply component to the gas-liquid input end of the gas-liquid mixing component until The pH of the gas-liquid output from the gas-liquid output end of the gas-liquid mixing assembly is restored to the given pH range.

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