CN111643713A - Preparation method of antibacterial absorbable medical periplaneta americana suture - Google Patents
Preparation method of antibacterial absorbable medical periplaneta americana suture Download PDFInfo
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- CN111643713A CN111643713A CN202010746554.1A CN202010746554A CN111643713A CN 111643713 A CN111643713 A CN 111643713A CN 202010746554 A CN202010746554 A CN 202010746554A CN 111643713 A CN111643713 A CN 111643713A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L17/00—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters
- A61L17/14—Post-treatment to improve physical properties
- A61L17/145—Coating
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L17/00—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters
- A61L17/005—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters containing a biologically active substance, e.g. a medicament or a biocide
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L17/00—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters
- A61L17/06—At least partially resorbable materials
- A61L17/08—At least partially resorbable materials of animal origin, e.g. catgut, collagen
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L17/00—Materials for surgical sutures or for ligaturing blood vessels ; Materials for prostheses or catheters
- A61L17/06—At least partially resorbable materials
- A61L17/10—At least partially resorbable materials containing macromolecular materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/30—Compounds of undetermined constitution extracted from natural sources, e.g. Aloe Vera
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
- A61L2300/604—Biodegradation
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- Surgery (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a preparation method of an antibacterial absorbable medical periplaneta americana suture, and relates to the technical field of medical sutures. The preparation steps are as follows: extracting the active ingredients of the periplaneta americana: soaking periplaneta americana in ethanol for three times, combining the three filtrates, carrying out reduced pressure distillation and concentration to obtain an extract, and carrying out petroleum ether degreasing treatment on the extract to obtain an periplaneta americana extract; preparing a chitosan fiber absorbable suture: pretreating chitosan fiber to prepare controllable degradable chitosan fiber and enhance the tensile strength of the chitosan fiber; synthesizing an antibacterial absorbable American cockroach suture: dissolving Periplaneta americana extract with ethanol, mixing with chitosan fiber, and oven drying. The invention combines the periplaneta Americana extract and the chitosan fiber, and the prepared suture line integrates the functions of antibiosis, detumescence, healing promotion and degradability, has good compatibility with human tissues and blood, high tensile strength and wide application prospect.
Description
Technical Field
The invention relates to the technical field of medical sutures, in particular to a preparation method of an antibacterial absorbable medical American cockroach suture.
Background
The medical suture is a linear material for human body surgical suture, and is mostly used for ligation hemostasis, suture hemostasis, tissue suture and the like in surgical operations. Existing medical sutures include both absorbable and non-absorbable sutures. The absorbable suture line used in the domestic hospital at present has the following two problems: after the materials used by the suture line are implanted into a human body, the implanted part can swell to different degrees and possibly cause acute inflammatory reaction; on the other hand, in the face of some wounds with relatively high tension, the tensile strength is not sufficient and may not be achieved, which may limit the use of the product.
The periplaneta americana extract can promote tissue repair and improve immunity, and has the effects of resisting inflammation and easing pain; the chitosan surgical suture is nontoxic, harmless and free of any side effect, is an excellent medical material which is easy to knot and absorb by organisms, and has a good application prospect from the existing research results, but the chitosan implant material which is clinically used at present is very limited, and the reason for the chitosan implant material is mainly three difficulties: firstly, the preparation process is limited, and no more effective processing method except that the preparation method is dissolved in a specific solvent exists at present; secondly, the product can not avoid the abnormal reaction of the organism with unknown reason after being implanted; thirdly, the degradation rate of the product is influenced by various factors (mainly enzymes) and cannot be controlled.
In summary, there is a need for an absorbable suture line with good compatibility with human tissues and blood and high tensile strength, and a preparation method of an antibacterial absorbable medical periplaneta americana suture line is especially necessary.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide the preparation method of the periplaneta americana antibacterial absorbable medical suture, the absorbable suture prepared by the method is formed by fusing the periplaneta americana extract and chitosan fiber, the implanted part does not have swelling and acute inflammatory reaction, the tensile strength is high, and the popularization and the use are easy.
In order to achieve the purpose, the invention is realized by the following technical scheme: the preparation method of the periplaneta americana antibacterial absorbable medical suture line comprises the following preparation steps:
(1) extracting the active ingredients of the periplaneta americana: soaking periplaneta americana in ethanol for three times, combining the three filtrates, performing conventional reduced pressure distillation and concentration to obtain an extract, and performing conventional petroleum ether degreasing treatment on the extract to obtain an ethanol extract of the periplaneta americana;
(2) preparing a chitosan fiber absorbable suture:
preprocessing chitosan fiber: pre-drying a chitosan fiber finished product, immersing the chitosan fiber finished product in a mixed solution of glutaraldehyde and dilute acetic acid for ultrasonic treatment, and finally, putting the stretched fiber in deionized water for soaking and drying;
② preparation of controlled degradation Chitosan fibers by immersing reinforced Chitosan fibers in Cu (OAc)2•H2Obtaining the controllable degraded chitosan fiber by using acetic acid solution of O;
enhancing the tensile strength of the chitosan fiber: stirring and dissolving carboxymethyl chitosan in water, performing vacuum defoaming treatment to prepare a crosslinked carboxymethyl chitosan sizing agent, immersing controllable degradable chitosan fibers into the crosslinked carboxymethyl chitosan sizing agent, uniformly sizing, drying, immersing into a calcium chloride aqueous solution for crosslinking, and drying;
(3) synthesizing an antibacterial absorbable American cockroach suture: dissolving Periplaneta americana extract with ethanol, mixing with chitosan fiber, and oven drying.
The invention has the beneficial effects that: the periplaneta americana extract and the chitosan fiber are combined to prepare the periplaneta americana antibacterial absorbable medical suture line, the antibacterial, detumescence, healing promotion and degradable functions are combined into a whole, swelling and acute inflammatory reaction do not exist at an implanted part, the compatibility with human tissues and blood is good, the tensile strength is high, and the application prospect is wide.
Drawings
The invention is described in detail below with reference to the drawings and the detailed description;
FIG. 1 is a flow chart of the preparation of the present invention.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Referring to fig. 1, the following technical solutions are adopted in the present embodiment: the preparation method of the periplaneta americana antibacterial absorbable medical suture line comprises the following preparation steps:
(1) extracting the active ingredients of the periplaneta americana: soaking periplaneta americana in ethanol for three times, combining the three filtrates, performing conventional reduced pressure distillation and concentration to obtain an extract, and performing conventional petroleum ether degreasing treatment on the extract to obtain an ethanol extract of the periplaneta americana;
(2) preparing a chitosan fiber absorbable suture:
preprocessing chitosan fiber: pre-drying a chitosan fiber finished product, immersing the chitosan fiber finished product in a mixed solution of glutaraldehyde and dilute acetic acid for ultrasonic treatment, and finally, putting the stretched fiber in deionized water for soaking and drying;
② preparation of controlled degradation Chitosan fibers by immersing reinforced Chitosan fibers in Cu (OAc)2•H2Obtaining the controllable degraded chitosan fiber by using acetic acid solution of O;
enhancing the tensile strength of the chitosan fiber: stirring and dissolving carboxymethyl chitosan in water, performing vacuum defoaming treatment to prepare a crosslinked carboxymethyl chitosan sizing agent, immersing controllable degradable chitosan fibers into the crosslinked carboxymethyl chitosan sizing agent, uniformly sizing, drying, immersing into a calcium chloride aqueous solution for crosslinking, and drying;
(3) synthesizing an antibacterial absorbable American cockroach suture: dissolving Periplaneta americana extract with ethanol, mixing with chitosan fiber, and oven drying.
The embodiment needs to perform tensile strength test, antigenicity research and in-vivo evaluation on the prepared periplaneta americana antibacterial absorbable medical suture, and the specific steps are as follows:
tensile strength test: and (3) measuring the axial elongation of the antibacterial absorbable American cockroach suture line during loading and unloading by using an FD-YC-II Young modulus determinator and a JCD3 type reading microscope by using a stretching method.
(II) antigenicity research of chitosan medical suture: sterilizing 1% chitosan acetic acid solution with NaOH, performing intraperitoneal injection on rabbit on the first, second, fourth and sixth days, collecting blood on day 15, collecting serum, and performing annular precipitation with serum and chitosan.
(III) in vivo evaluation:
i) tissue sections
50 male nude mice were divided into 3 groups and sutured to the sacral spinal muscles of the mice. The sheep intestine group was sutured and the control group was sutured with needles without thread, and then the mice were sacrificed at a prescribed time to take out the muscle tissue section at the sutured site for treatment, staining, and microscopic observation.
Ii) scanning by electron microscope
Fixing the sutured muscle with n-butanol by glutaraldehyde, dehydrating, spraying gold in vacuum, observing and photographing by a scanning electron microscope, taking the buttock muscle of the mouse, quickly freezing in liquid nitrogen, grinding in an ice bath, centrifuging, removing precipitate, and analyzing the supernatant by isozyme.
Iii) evaluation of antibacterial Effect
Two rabbits, male and female, were anesthetized, sheared, and the skin was incised and sutured with catgut and periplaneta americana antibacterial absorbable suture. Before suturing, blood was taken from the vein, and white blood cells were counted and classified, and then the above experiment was also performed at a predetermined time after suturing.
The medical absorbable suture line is formed by fusing the periplaneta americana extract and the chitosan fiber, combines the periplaneta americana extract and the chitosan fiber, integrates the functions of antibiosis, detumescence, healing promotion and degradability into a whole, and is prepared into the medical absorbable suture line which can be widely used clinically, wherein:
(1) the periplaneta americana extract is characterized by healing effect, and has the effects of resisting bacteria, reducing swelling, stopping bleeding, easing pain and promoting skin healing when contacting with skin, so that the wound surface can better absorb the medicine, the skin tissue recovery is accelerated, and the wound healing and regeneration capacity is improved.
(2) The chitosan fiber is a green fiber which is easy to biodegrade and is the only positive cation natural fiber in nature, has equivalent biological activity and biocompatibility and also has the following functions: firstly, the enzyme can be decomposed and absorbed; dissolving in acid solution to form positive charged cationic group; ③ the human cells have strong affinity; fourthly, the dissolved chitosan is in a gel state and has stronger adsorption capacity; the chitosan is natural cellulose, has no toxicity and side effect, and has high safety.
The specific implementation mode combines the active ingredients containing the periplaneta americana with the controllable degradable medical suture, so that the suture not only has an absorbable function, but also has the effects of resisting bacteria, reducing swelling, relieving pain and the like, the defects of the existing suture are improved, the problems of swelling, acute inflammation and the like caused by the existing suture are solved, the degradable suture also has a degradable function, the suture has excellent performance, the defects of the catgut and the collagen line are overcome, and the suture has the advantages of high breaking strength, small tissue reaction, good absorption effect and the like, has potential social benefits and economic benefits, can be widely applied clinically, and has wide application prospects.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (1)
1. The preparation method of the periplaneta americana antibacterial absorbable medical suture is characterized by comprising the following preparation steps:
(1) extracting the active ingredients of the periplaneta americana: soaking periplaneta americana in ethanol for three times, combining the three filtrates, carrying out reduced pressure distillation and concentration to obtain an extract, and carrying out petroleum ether degreasing treatment on the extract to obtain an periplaneta americana extract;
(2) preparing a chitosan fiber absorbable suture:
preprocessing chitosan fiber: pre-drying a chitosan fiber finished product, immersing the chitosan fiber finished product in a mixed solution of glutaraldehyde and dilute acetic acid for ultrasonic treatment, and finally, putting the stretched fiber in deionized water for soaking and drying;
② preparation of controllable degradable shell polymerSugar fiber: immersing the reinforced chitosan fiber in Cu (OAc)2•H2Obtaining the controllable degraded chitosan fiber by using acetic acid solution of O;
enhancing the tensile strength of the chitosan fiber: stirring and dissolving carboxymethyl chitosan in water, performing vacuum defoaming treatment to prepare a crosslinked carboxymethyl chitosan sizing agent, immersing controllable degradable chitosan fibers into the crosslinked carboxymethyl chitosan sizing agent, uniformly sizing, drying, immersing into a calcium chloride aqueous solution for crosslinking, and drying;
(3) synthesizing an antibacterial absorbable American cockroach suture: dissolving Periplaneta americana extract with ethanol, mixing with chitosan fiber, and oven drying.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103446187A (en) * | 2013-09-05 | 2013-12-18 | 陈光健 | Anti-inflammation and detumescent pharmaceutical composition and preparation method and applications thereof |
| CN108379643A (en) * | 2018-02-13 | 2018-08-10 | 云南宁康生物科技有限公司 | A kind of hydrogel woundplast of the active constituent containing American cockroach |
| CN108642858A (en) * | 2018-04-26 | 2018-10-12 | 海斯摩尔生物科技有限公司 | A kind of preparation method of controlled degradation suture |
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| CN108653795A (en) * | 2018-04-26 | 2018-10-16 | 东华大学 | A kind of preparation method carrying the high-strength suture of medicine |
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