CN111638325A

CN111638325A - Antibody incubation box

Info

Publication number: CN111638325A
Application number: CN202010584071.6A
Authority: CN
Inventors: 不公告发明人
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-06-24
Filing date: 2020-06-24
Publication date: 2020-09-08

Abstract

The invention relates to the technical field of antibody cultivation, in particular to an antibody incubation box which comprises a box body, wherein a plurality of incubation grooves are formed in the box body, a baffle is movably connected to an opening of each incubation groove, the baffle is independently arranged on each incubation groove, when the baffle on one incubation groove is opened, other baffles are still sealed on the incubation grooves, pollutants in air are prevented from entering other incubation grooves, when a dropper is used for dropwise adding substances required by antibodies into one incubation groove, liquid on the dropper accidentally slides down and can be blocked by the baffles, and pollution to other incubation grooves is prevented.

Description

Antibody incubation box

Technical Field

The invention relates to the technical field of antibody cultivation, in particular to an antibody incubation box.

Background

With the widespread development of molecular biology techniques, western blotting has become a technique frequently used in every laboratory. The quality of the antibody, among other things, determines the experimental results. A commercial antibody is often expensive, and finding an antibody incubation cassette that is not easily wasted is a problem faced by almost every researcher. The antibody incubation box is one of important experimental devices for detecting protein content in biological experiments.

In order to prevent the waste caused by the contamination of the antibodies in the incubation box, the structure of the existing incubation box is shown in fig. 1, the inside of the incubation box 1 is divided into a plurality of antibody incubation chambers by partitions 101, a box cover 102 is hinged on the incubation box 1, and the box cover 102 is closed during the antibody incubation, so as to prevent the contaminants such as impurities, microorganisms and the like in the air from entering the incubation chambers.

However, during the incubation period of partial antibodies, experimenters need to use a dropper to drop nutrient solutions and inorganic solutions with different concentrations or types according to the incubation conditions of the antibodies in different incubation chambers, and during the period of dropping the nutrient solutions and the inorganic solutions required by antibody splitting in the incubation box by opening the box cover, pollutants in the air may possibly enter the incubation chambers to cause antibody pollution, and partial liquids attached to the inner part and the outer wall of the dropper may also accidentally fall into other incubation chambers to cause antibody pollution.

Disclosure of Invention

The invention aims to solve the defect that the antibody in an incubation box is polluted in the process of culturing, and provides the antibody incubation box.

In order to achieve the purpose, the invention adopts the following technical scheme:

the design is an antibody incubation box, which comprises a box body, wherein a plurality of incubation grooves are arranged in the box body, and baffles are movably connected at the opening parts of the incubation grooves.

Preferably, the frame is installed to the box body up end the inside rigid coupling of frame has the pivot, the cover is equipped with a plurality of gears in the pivot, baffle one side rigid coupling is in on the gear, a plurality of guide ways have been seted up on the frame inside wall, it is located to incubate the trench the pivot with between the frame inner wall, the rack is installed to incubation groove one side, the guide block is installed to incubation groove opposite side, the rack can be engaged in on the gear, guide block slidable connects in the guide way.

Preferably, the upper surface of the baffle is provided with a groove.

Preferably, the baffle plate is provided with a flow channel, and an included angle with an acute angle is formed between the central axis of the flow channel and the baffle plate.

Preferably, an elastic plug is hermetically connected to the flow channel, a perforation is formed in the elastic plug and communicated with the flow channel, a plurality of valves are installed in the perforation, and the valves can be sealed in the perforation.

Preferably, the ejecting structure of box body internally mounted, ejecting structure can drive the incubation groove reciprocates, ejecting structure includes threaded rod, tooth post, internal thread pipe, sliding sleeve, slide bar, movable rack and wedge, the threaded rod with the rotatable installation of tooth post in the box body, the threaded rod with the axis of tooth post is parallel, internal thread pipe spiro union is in on the threaded rod, the sliding sleeve welding is at internal thread nose portion, the horizontal cartridge of slide bar is in the sliding sleeve, the slide bar is in removal orbit perpendicular to in the sliding sleeve the axis of threaded rod, movable rack with the slide bar rigid coupling is in the same place, the meshing of movable rack is in on the tooth post, the wedge is installed on the slide bar, the maximum height of wedge is greater than the height of incubation bottom surface tank.

Preferably, hatch inslot mobilizable piston plate of installing, install the layer board on the piston plate bottom surface, it has the second cylinder to hatch inslot portion bottom surface horizontal installation, slidable installs the second piston in the second cylinder, install the expansion pipe on the piston, threaded connection has accommodate the lead screw in the expansion pipe, accommodate the lead screw is rotatable to be installed on the incubation groove, lead screw one end is run through incubation groove lateral wall, vertical intercommunication has first cylinder on the second cylinder, movable mounting has first piston in the first cylinder, install the movable rod on the first piston, the top welding of movable rod is in on the layer board bottom surface.

The antibody incubation box provided by the invention has the beneficial effects that: this box is hatched to antibody all is provided with the baffle alone at every incubation groove, and when the baffle on certain incubation groove was opened, other baffles still sealed on incubation groove, prevent that the pollutant in the air from getting into other incubation grooves, when using the burette to the required material of certain incubation groove dropwise add antibody, can be blocked by the baffle when the liquid on the burette unexpected landing, prevent to cause the pollution to other incubation grooves.

Drawings

FIG. 1 is a schematic structural diagram of a conventional antibody incubation cassette.

FIG. 2 is a schematic structural diagram of an antibody incubation box according to the present invention.

FIG. 3 is a schematic diagram of the internal structure of an antibody incubation box according to the present invention.

FIG. 4 is a schematic structural diagram of a framework of an antibody incubation cassette according to the present invention.

FIG. 5 is a schematic structural diagram of a baffle plate of an antibody incubation box according to the present invention.

FIG. 6 is a cross-sectional view of a baffle plate of an antibody incubation cassette according to the present invention.

FIG. 7 is a schematic structural diagram of an incubation groove of an antibody incubation box according to the present invention.

FIG. 8 is a schematic structural diagram of an incubation groove of an antibody incubation box according to the present invention.

Fig. 9 is a schematic structural diagram of an ejection structure of an incubation groove of an antibody incubation box according to the present invention.

FIG. 10 is a schematic structural diagram of an incubation groove of an antibody incubation box according to the present invention.

FIG. 11 is a partially enlarged view of an incubation well of an antibody incubation cassette according to the present invention.

FIG. 12 is an enlarged view of the incubation groove A of the antibody incubation box of the present invention.

Fig. 13 is a structural schematic diagram of a valve sealing state of an incubation groove of an antibody incubation box provided by the invention.

In the figure: incubation box 1, partition board 101, box cover 102, box body 2, frame 3, sealing strip 4, guide groove 5, rotating shaft 6, gear 7, baffle 8, flow channel 801, elastic plug 802, groove 803, incubation groove 9, piston plate 901, supporting plate 902, movable rod 903, first piston 904, first cylinder 905, second cylinder 906, second piston 907, movable pipe 908, adjusting screw rod 909, rack 10, guide block 11, threaded rod 12, toothed column 13, internal threaded pipe 14, sliding sleeve 15, handle 16, sliding rod 17, movable toothed strip 18, wedge 19,

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Example 1

Referring to fig. 2-3, an antibody incubation box, including box body 2, be equipped with a plurality of incubation grooves 9 in box body 2, movably be connected with baffle 8 at the opening part of incubation groove 9, every incubation groove 9 all is provided with baffle 8 alone, when baffle 8 on a certain incubation groove 9 is opened, other baffle 8 still seal on incubation groove 9, prevent that the pollutant in the air from getting into other incubation grooves 9 in, when using the burette to the required material of a certain incubation groove 9 dropwise add antibody, can be blocked by baffle 8 when the liquid on the burette is unexpected the landing, prevent to cause the pollution to other incubation grooves 9.

Example 2

On the basis of the above embodiment 1, in this embodiment, the frame 3 is installed on the upper end face of the box body 2, as shown in fig. 4-8, the rotating shaft 6 is fixedly connected inside the frame 3, the rotating shaft 6 is sleeved with the plurality of gears 7, the area of the baffle 8 is larger than that of the upper end face of the incubation groove 9, according to the position of the corresponding incubation groove 9, one side of the baffle 8 parallel to the rotating shaft 6 is fixedly connected to the gears 7 when being sealed on the incubation groove 9, the inner side wall of the frame 3 is provided with the plurality of guide grooves 5, the incubation groove 9 is located between the rotating shaft 6 and the inner wall of the frame 3, the incubation grooves 9 are separated by the sealing strips 4, the dripped liquid is prevented from falling into the box body 2 from the gap, the rack 10 is installed on one side of the incubation groove 9, the guide block 11 is installed on the other side of the incubation groove 9, the rack. When the incubation groove 9 which is independently arranged is arranged in the frame 3, the guide block 11 is matched with the guide groove 5 to prevent the incubation groove 9 from deviating, the rack 10 is matched with the gear 7, in the process that the incubation groove 9 moves downwards under the gravity, the rack 10 drives the gear 7 to rotate until the baffle 8 completely covers the opening of the incubation groove 9, after the tooth blocks on the rack 10 are all meshed with the gear 7, the subsequent toothless blocks of the gear 7 are meshed, the gear 7 at the moment can continuously abut against the edge of the incubation groove 9, the incubation groove 9 can continuously give downward pressure to the rack 10 due to the gravity of the self and the gravity of the internal liquid, when the gear 7 receives the downward pressure given by the rack 10, the pressure of the surface where the opening of the incubation groove 9 is vertical to the baffle 8 in one direction can be given, and the baffle 8 is prevented from being accidentally opened due to the disturbance of the airflow above.

Example 3

In this embodiment, as shown in fig. 5 to 6, grooves 803 are formed in the upper surface of the baffle plate 8 in addition to embodiment 1 or embodiment 2, the liquid dropped on the baffle plate 8 is collected by the grooves 803, and the liquid collected in the grooves 803 can be cleaned by cotton cloth or cotton balls.

Example 4

Referring to fig. 5 to 13, in addition to embodiment 1, embodiment 2, or embodiment 3, in this embodiment, a flow channel 801 is formed in the baffle 8, and when a liquid is dropped into the incubation groove 9 by using a dropper, the liquid can be dropped through the flow channel 801, and when the liquid is dropped by opening the baffle 8, contaminants in the air can be prevented from entering the incubation groove 9, in order to prevent the contaminants in the air from entering the incubation groove 9 through the flow channel, the flow channel 801 is obliquely arranged, an elastic plug 802 is hermetically connected to the flow channel 801, a perforation 20 is formed inside the elastic plug 802, the perforation 20 is communicated with the flow channel 801, a plurality of valves 21 are installed in the perforation 20, when the valves 21 are combined together, the perforation 20 is sealed, when an experimenter drops the liquid by using the dropper, a head of the dropper opens the valves 21 along the perforation 20, and enters the flow channel 801 after passing through the valves 21.

Example 5

On the basis of embodiment 2, the present embodiment installs an ejection structure inside the box body 2, as shown in fig. 3-9, the ejection structure can drive the incubation groove 9 to move up and down, the ejection structure includes a threaded rod 12, a tooth post 13, an internally threaded tube 14, a sliding sleeve 15, a sliding rod 17, a movable rack 18 and a wedge block 19, the threaded rod 12 and the tooth post 13 are rotatably installed inside the box body 2, the axes of the threaded rod 12 and the tooth post 13 are parallel, the internally threaded tube 14 is screwed on the threaded rod 12, the sliding sleeve 15 is welded on the top of the internally threaded tube 14, the sliding rod 17 is horizontally inserted in the sliding sleeve 15, the moving track of the sliding rod 17 in the sliding sleeve 15 is perpendicular to the axis of the threaded rod 12, the movable rack 18 and the sliding rod 17 are fixedly connected, the movable rack 18 is engaged on the tooth post 13, the wedge block 19 is installed on the sliding rod 17, the maximum height of the wedge block 19 is greater than the height of the bottom, can drive threaded rod 12 and tooth post 13 rotatory through handle 16, when needing to open a certain incubation groove 9, drive threaded rod 12 is rotatory, threaded rod 12 is rotatory can drive internal thread pipe 14 along threaded rod 12 axis removal, thereby drive wedge 19 longitudinal movement, when wedge 19 moves to wedge 19 on horizontal projection is located the incubation groove 9 that will open completely, stop rotating threaded rod 12 and drive tooth post 13 and rotate, the rotation of tooth post 13 can drive movable rack 18 lateral shifting, the moving direction of control movable rack 18, make wedge 19 move to incubation groove 9 below and can be ejecting with the incubation groove 9 that needs open.

Example 6

On the basis of embodiment 1, in this embodiment, as shown in fig. 7 to 11, a piston plate 901 is movably installed in an incubation groove 9, a supporting plate 902 is installed on the bottom surface of the piston plate 901, a second cylinder 906 is horizontally installed on the bottom surface inside the incubation groove 9, a second piston 907 is slidably installed in the second cylinder 906, a movable tube 908 is installed on the piston 907, an adjusting screw 909 is threadedly connected to the movable tube 908, the adjusting screw 909 is rotatably installed on the incubation groove 9, one end of the screw 909 penetrates through the side wall of the incubation groove 9, a first cylinder 905 is vertically communicated with the second cylinder 906, a first piston 904 is movably installed in the first cylinder 905, a movable rod 903 is installed on the first piston 904, the top end of the movable rod 903 is welded to the bottom surface of the supporting plate 902, the air pressure intensity in the second cylinder 906 can be changed by rotating the adjusting screw 909 to drive the movable tube 908 to horizontally move, the first cylinder 904 is communicated with the second cylinder 906, when the pressure in the second air cylinder 906 changes, the pressure in the first air cylinder 905 also changes, and the change of the pressure can make the movable rod 903 move up and down;

when the pressure in the second cylinder 906 increases, the movable rod 903 moves upwards to drive the piston plate 901 to move upwards, and residual antibodies and impurities attached to the inner wall of the incubation groove 9 can be cleaned when the piston plate 901 moves upwards;

when the pressure in the second cylinder 906 decreases, the movable rod 903 moves down to drive the piston plate 901 to move downward, so that the volume inside the incubation groove 9 increases, and the antibody accommodating capacity in the incubation groove 9 is increased.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims

1. The utility model provides a box is hatched to antibody, includes box body (2), its characterized in that be equipped with a plurality of incubation groove (9) in box body (2) the opening part mobilizable baffle (8) that is connected with of incubation groove (9).

2. The antibody incubation box according to claim 1, wherein a frame (3) is installed on the upper end face of the box body (2), a rotating shaft (6) is fixedly connected to the inside of the frame (3), a plurality of gears (7) are sleeved on the rotating shaft (6), one side of the baffle (8) is fixedly connected to the gears (7), a plurality of guide grooves (5) are formed in the inner side wall of the frame (3), the incubation groove (9) is located between the rotating shaft (6) and the inner wall of the frame (3), a rack (10) is installed on one side of the incubation groove (9), a guide block (11) is installed on the other side of the incubation groove (9), the rack (10) can be meshed to the gears (7), and the guide block (11) is slidably connected to the inside of the guide grooves (5).

3. An antibody incubation cassette according to claim 1, wherein the upper surface of the baffle (8) is provided with a recess (803).

4. The antibody incubation box according to claim 1, wherein the baffle (8) is provided with a flow channel (801), and an included angle with an acute angle is formed between a central axis of the flow channel (801) and the baffle (8).

5. An antibody incubation box according to claim 1, wherein an elastic plug (802) is hermetically connected to the flow channel (801), a perforation (20) is formed inside the elastic plug (802), the perforation (20) is communicated with the flow channel (801), a plurality of valves (21) are installed inside the perforation (20), and the valves (21) are sealable inside the perforation (20).

6. An antibody incubation box according to claim 1, characterized in that the inside of the box body (2) is provided with an ejection structure, the ejection structure can drive the incubation groove (9) to move up and down, the ejection structure comprises a threaded rod (12), a toothed column (13), an internally threaded tube (14), a sliding sleeve (15), a sliding rod (17), a movable rack (18) and a wedge block (19), the threaded rod (12) and the toothed column (13) are rotatably arranged in the box body (2), the axes of the threaded rod (12) and the toothed column (13) are parallel, the internally threaded tube (14) is screwed on the threaded rod (12), the sliding sleeve (15) is welded on the top of the internally threaded tube (14), the sliding rod (17) is horizontally inserted in the sliding sleeve (15), the moving track of the sliding rod (17) in the sliding sleeve (15) is perpendicular to the axis of the threaded rod (12), the movable rack (18) is fixedly connected with the sliding rod (17), the movable rack (18) is meshed with the toothed column (13), the wedge block (19) is installed on the sliding rod (17), and the maximum height of the wedge block (19) is greater than the height of the bottom surface of the incubation groove (9).

7. The antibody incubation box according to claim 1, wherein a piston plate (901) is movably mounted in the incubation groove (9), a supporting plate (902) is mounted on the bottom surface of the piston plate (901), a second cylinder (906) is horizontally mounted on the bottom surface of the inside of the incubation groove (9), a second piston (9074) is slidably mounted in the second cylinder (906), a movable tube (908) is mounted on the piston (907), an adjusting screw rod (909) is screwed in the movable tube (908), the adjusting screw rod (909) is rotatably mounted on the incubation groove (9), one end of the screw rod (909) penetrates through the side wall of the incubation groove (9), a first cylinder (905) is vertically communicated with the second cylinder (906), a first piston (904) is movably mounted in the first cylinder (905), and a movable rod (903) is mounted on the first piston (904), the top end of the movable rod (903) is welded on the bottom surface of the supporting plate (902).