Constant-temperature multi-oscillation water bath shaking table
Technical Field
The invention relates to the technical field of water bath shaking tables, in particular to a constant-temperature multi-oscillation water bath shaking table.
Background
The water bath shaking table is a biochemical instrument combining a temperature-controllable constant-temperature water bath and an oscillator, and is mainly suitable for various scientific research departments such as universities and colleges, petrochemical industry, epidemic prevention, environmental monitoring and the like to be used for shaking culture of various liquid and solid-liquid mixtures such as biology, biochemistry, cells, strains and the like; shaking culture, also known as shake flask culture, refers to a culture method in which microbial cells are inoculated in a liquid culture medium and placed on a shaking table or a shaker for continuous shaking, shaking enables the culture medium to be fully contacted with oxygen, increases the supply of dissolved oxygen, and the thalli cultured by shaking reproduce uniformly, have high culture efficiency, and are widely applied to strain screening and microbial scale-up culture, but the water bath shaking table used at present has some defects as follows:
at present, when the microbial cells are cultured by shaking, the liquid medium containing the microbial cells is placed on a shaking table of a water bath shaker, but in general, the containers used for containing the culture medium are different in size and quantity, no special fixture is arranged on the oscillating platform for fixing the containers, the containers need to be clamped by customizing a special fixing fixture, the containers are prevented from being displaced and colliding with each other in the oscillating process to cause the damage of the containers, the cost for customizing the clamp is higher, meanwhile, the water bath shaking table provides oscillation through the oscillator, but the oscillation effect conducted to the culture medium is greatly reduced due to the damping effect of the water in the water bath shaking table in the oscillation process, and the effective oscillation cannot be carried out, meanwhile, once the amplitude is too large, water is easy to shake and fluctuate violently, and is easy to splash into a culture medium, so that the culture medium is damaged.
Disclosure of Invention
The invention provides a constant-temperature multi-oscillation water bath shaking table which has the advantages of good oscillation effect and no need of an additional clamp, and solves the problems in the background art.
The invention provides the following technical scheme: a constant-temperature multi-oscillation water bath shaking table comprises a fixed box body, a culture medium and a heating device, wherein the heating device is fixedly arranged in the fixed box body, a lower transmission structure is movably arranged at the left bottom of an inner cavity of the fixed box body, an upper transmission structure is movably arranged at the left top of the inner cavity of the fixed box body, a fixing frame is fixedly connected at the right end of the lower transmission structure, an X-shaped bottom plate is fixedly arranged at the bottom of the fixing frame, a hydraulic oil cavity is fixedly arranged at the center of the bottom of the X-shaped bottom plate, a supporting device is movably sleeved at the center of the hydraulic oil cavity, an aligning plate is fixedly connected at the top end of the supporting device, four corners of the aligning plate are movably sleeved with the fixing frame respectively, a fixed plate is fixedly connected at the middle part of the fixing frame, an oscillation device is movably sleeved on the fixed plate and arranged in an array manner, and an inflation tube is fixedly connected at the left side of the bottom of the oscillation device, oscillation device's bottom right side fixedly connected with electric wire, the right side top movable mounting of mount has the connecting rod, the bottom of connecting rod and the bottom swing joint on mount right side, the right side fixed mounting of fixed box has the syringe, the bottom center fixedly connected with oil pipe of syringe, oil pipe's left end and hydraulic pressure oil pocket intercommunication, the inner chamber left side fixed mounting of fixed box has servo motor, servo motor has two, and respectively with lower transmission structure and last transmission structure fixed connection, the left side of fixed box inner chamber and the place ahead fixed mounting who is located servo motor have the pump, the pump is fixed with the gas tube and is communicated, culture medium and oscillation device swing joint.
Preferably, oscillation device includes the fixed section of thick bamboo in center, the inside fixed mounting of the fixed section of thick bamboo in center has vibrating motor, outside rubber sleeve has been cup jointed to the outside of the fixed section of thick bamboo in center, vibrating motor's bottom and electric wire fixed connection, outside rubber sleeve is including fixed cover and inflation cover, the fixed cover that has cup jointed in the top third department of the fixed section of thick bamboo in center, the inflation cover has been cup jointed in the activity of two-thirds in the fixed section of thick bamboo lower part in center, the inner chamber bottom surface of inflation cover and the bottom surface fixed connection of the fixed section of thick bamboo in center, the space intercommunication between gas tube and inflation cover and the fixed section of thick bamboo in center.
Preferably, the outer rubber sleeve is made of high-temperature-resistant rubber, and the oscillating device is in interference fit with the fixing plate.
Preferably, the lower transmission structure comprises a turntable, a distance device is movably mounted on the turntable, a transmission rod is movably sleeved on the distance device, and a movable plate is movably sleeved at the right end of the transmission rod.
Preferably, the lower transmission structure is the same as the upper transmission structure in specification and consistent in motion law, a water separation sleeve is fixedly mounted at the right end of the upper transmission structure, the other end of the water separation sleeve is fixedly connected with the inner wall of the fixed box body, a passage opening between the fixed box body and the upper transmission structure is sealed by the water separation sleeve, and the water separation sleeve is made of elastic high-temperature-resistant rubber.
Preferably, the hydraulic oil chamber and the injector are filled with hydraulic oil.
Preferably, the supporting device comprises four supporting sleeves, and through holes which are uniformly distributed are formed in the bottoms of the supporting sleeves.
The invention has the following beneficial effects:
1. through the setting of oscillation device and fixed plate, can place oscillation device's upper surface with the culture medium, make oscillation device can take place relative displacement according to the shape and the size of culture medium and fixed plate, slide down, and then the culture medium is inlayed between oscillation device, and then by fixed clamp, reduce the use of anchor clamps, can realize carrying out the clamping to the culture medium of multiple specification, reduce the expense of customization anchor clamps, reduce test cost, simultaneously because oscillation device is array arrangement, and then can carry out the clamping to a plurality of culture mediums, increase the culture medium quantity that once only needs the oscillation culture, increase of production, improve culture efficiency.
2. Through vibrating motor's setting, make can the selectivity open the oscillation device with the culture medium contact, carry out the contact oscillation to the culture medium, and reduce the energy attenuation of oscillation in aqueous, improve the oscillation effect, and through direct contact, effectively reduce the surging that causes water, can reduce the risk that the splash that surges gets into in the culture medium, improve the security of device, simultaneously through the setting of underdrive structure and last drive structure, make the device have multiple oscillation form and oscillation amplitude, and then improve the functionality of device, can satisfy multiple needs.
Drawings
FIG. 1 is a schematic front view of a half-section of the structure of the present invention;
FIG. 2 is a schematic top view of the structure of the present invention;
FIG. 3 is a schematic view of the installation of the culture medium in the structure of the present invention;
FIG. 4 is a schematic view of an oscillation device of the present invention;
FIG. 5 is a schematic view of the working state of the oscillation device of the present invention;
FIG. 6 is a schematic view of the installation of the structural X-shaped base plate and the supporting device of the present invention;
FIG. 7 is an enlarged view of the point A in FIG. 1;
FIG. 8 is an enlarged view of the point B in FIG. 1.
In the figure: 1. fixing the box body; 2. a lower transmission structure; 21. a turntable; 22. a distance device; 23. a transmission rod; 24. a movable plate; 3. an upper transmission structure; 4. a fixed mount; 5. an X-shaped bottom plate; 6. a hydraulic oil chamber; 7. a support device; 71. a support sleeve; 72. an oil through hole; 8. trimming the plate; 9. a fixing plate; 10. an oscillation device; 101. a central fixed cylinder; 102. an outer rubber sleeve; 1021. fixing a sleeve; 1022. an expansion sleeve; 103. a vibration motor; 11. an inflation tube; 12. an electric wire; 13. a connecting rod; 14. an injector; 15. an oil pipe; 16. a servo motor; 17. an inflator pump; 18. a culture medium; 19. a water-isolating sleeve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-8, a constant temperature multi-oscillation water bath shaking table comprises a fixed box 1, a culture medium 18 and a heating device, wherein the heating device is fixedly arranged inside the fixed box 1, a lower transmission structure 2 is movably arranged at the bottom of the left side of the inner cavity of the fixed box 1, an upper transmission structure 3 is movably arranged at the top of the left side of the inner cavity of the fixed box 1, a fixed frame 4 is fixedly connected with the right end of the lower transmission structure 2, an X-shaped bottom plate 5 is fixedly arranged at the bottom of the fixed frame 4, a hydraulic oil cavity 6 is fixedly arranged at the center of the bottom of the X-shaped bottom plate 5, a supporting device 7 is movably sleeved at the center of the hydraulic oil cavity 6, an aligning plate 8 is fixedly connected at the top of the supporting device 7, four corners of the aligning plate 8 are respectively movably sleeved with the fixed frame 4, a fixed plate 9 is fixedly connected with the middle part of the fixed frame 4, an oscillation device 10 is movably sleeved on the fixed plate 9 and is arranged in an array form, the utility model discloses an oscillation device 10, including vibrating device 10, bottom left side fixedly connected with gas tube 11, vibrating device 10's bottom right side fixedly connected with electric wire 12, the right side top movable mounting of mount 4 has connecting rod 13, the bottom of connecting rod 13 and the bottom swing joint on mount 4 right side, the right side fixed mounting of fixed box 1 has syringe 14, the bottom center fixedly connected with oil pipe 15 of syringe 14, the left end and the hydraulic pressure oil chamber 6 intercommunication of oil pipe 15, the inner chamber left side fixed mounting of fixed box 1 has servo motor 16, servo motor 16 has two, and respectively with lower drive structure 2 and last drive structure 3 fixed connection, the left side of fixed box 1 inner chamber and the place ahead fixed mounting that is located servo motor 16 have inflator pump 17, inflator pump 17 and the fixed intercommunication of gas tube 11, culture medium 18 and vibrating device 10 swing joint.
Wherein, the oscillation device 10 comprises a central fixed cylinder 101, a vibration motor 103 is fixedly arranged inside the central fixed cylinder 101, so that contact-type oscillation is carried out on three surfaces of the culture medium 18, the energy attenuation of the oscillation in water is reduced, the oscillation effect is improved, and through direct contact, the sloshing caused to water is effectively reduced, the risk that slops of the sloshing water enter the culture medium 18 can be reduced, the safety of the device is improved, an external rubber sleeve 102 is sleeved outside the central fixed cylinder 101, the bottom end of the vibration motor 103 is fixedly connected with an electric wire 12, the external rubber sleeve 102 comprises a fixed sleeve 1021 and an expansion sleeve 1022, the fixed sleeve 1021 is fixedly sleeved at one third of the top end of the central fixed cylinder 101, the expansion sleeve 1022 is movably sleeved at two thirds of the lower part of the central fixed cylinder 101, the bottom surface of an inner cavity of the expansion sleeve is fixedly connected with the bottom surface of the central fixed cylinder 101, the inflation tube 11 is communicated with the space between the expansion sleeve 1022 and the central fixed cylinder 101, the inflator pump 17 inflates air between the central fixed cylinder 101 and the expansion sleeve 1022 through the inflation tube 11, so that the expansion sleeve 1022 expands, the culture medium 18 is clamped and fixed on one hand, the contact force with the fixed plate 9 is increased on the other hand, the oscillation device 10 is prevented from sliding in the oscillation process, meanwhile, the oscillation motors 103 can be selectively started according to the number and the installation positions of the culture medium 18, the oscillation of the culture medium 18 can be met, the energy consumption caused by the simultaneous starting of too many oscillation motors 103 is reduced, the oscillation frequency of the oscillation motors 103 is controlled, and the too many oscillation motors 103 are prevented from oscillating together to cause water sloshing.
Wherein, outside rubber sleeve 102 is made by high temperature resistant rubber, and oscillation device 10 and fixed plate 9 interference fit, when oscillation device 10 cup jointed with fixed plate 9, frictional force between the two was greater than its self gravity to outside rubber sleeve 102 inflation can effectively increase the extrusion force to culture medium 18, guarantees that culture medium 18 is fixed by safety.
Wherein, underdrive structure 2 includes carousel 21, and movable mounting has distance device 22 on the carousel 21, and distance device 22 is gone up the activity and is cup jointed transfer line 23, and the right-hand member activity of transfer line 23 is cup jointed fly leaf 24, through the transmission between its four, gives mount 4 with servo motor 16's power transmission, drives culture medium 18 and oscillates to make the device have multiple oscillation form and oscillation amplitude, and then improve the functionality of device, can satisfy multiple needs.
Wherein, the specification of lower drive structure 2 is the same with last drive structure 3, and the motion law is unanimous, and the right-hand member fixed mounting of last drive structure 3 has water proof cover 19, and the other end of water proof cover 19 and the inner wall fixed connection of fixed box 1, and water proof cover 19 seals the passway mouth between fixed box 1 and the last drive structure 3, and water proof cover 19 is the resistant high temperature rubber of elasticity and makes, can prevent that the hot water of fixed box 1 from invading the space at servo motor 16 place, protects servo motor 16.
The hydraulic oil chamber 6 and the injector 14 are filled with hydraulic oil.
The supporting device 7 comprises supporting sleeves 71, the supporting sleeves 71 are four sleeves, through holes are uniformly distributed in the bottoms of the supporting sleeves 71, and when hydraulic oil enters the hydraulic oil cavity 6 from the injector 14, the supporting device 7 is prevented from being combined with the bottom of the hydraulic oil cavity 6, and hydraulic oil cannot enter the supporting device 7.
The working principle is that firstly, the injector 14 is pressed, the injector 14 presses the hydraulic oil in the injector into the hydraulic oil cavity 6 through the oil pipe 15, the supporting device 7 is ejected out, the supporting device 7 extends out, the tidying plate 8 is further upwards, the oscillating device 10 is completely ejected upwards and is arranged neatly, then the culture medium 18 is placed on the upper surface of the oscillating device 10, at the moment, the injector 14 is pulled, the hydraulic oil is drawn back, the tidying plate 8 descends, at the moment, the culture medium 18 is lightly pressed, the oscillating device 10 at the bottom of the culture medium 18 descends, the culture medium 18 is sunk between the oscillating devices 10, after the culture medium 18 is installed in place, the inflator pump 17 inflates air between the central fixing cylinder 101 and the expansion sleeve 1022 through the inflation pipe 11, the expansion sleeve 1022 is inflated, on one hand, the culture medium 18 is clamped and fixed, on the other hand, the contact force with the fixing plate 9 is increased, and the vibration process is prevented, the oscillation device 10 slips off, and after the installation, add distilled water to fixed box 1 to heat to required temperature, then keep warm, select the oscillation mode at last, as follows:
in the first oscillation mode, the electric wire 12 is used for starting the vibration motor 103, so that the vibration motor 103 eccentrically rotates in the central fixed cylinder 101, the oscillation device 10 is driven to vibrate, and three-side high-frequency low-amplitude vibration is performed on the culture medium 18;
in the second oscillation mode, the servo motor 16 drives the fixed frame 4 to rotate 31, and then the fixed frame 4 sequentially drives the distance device 22, the transmission rod 23 and the movable plate 24 to perform reciprocating parallel movement, so that the culture medium 18 performs low-frequency high-amplitude vibration.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.