CN111607508A - An automatic mesenchymal stem cell separation device - Google Patents

Abstract

The invention provides an automatic mesenchymal stem cell separation device which comprises a shell, a controller arranged outside the shell, a tissue separator arranged inside the shell, and a motor used for driving the tissue separator to operate, wherein a plurality of liquid storage tanks are formed between the tissue separator and the shell, a liquid inlet pipeline and a liquid discharge pipeline are arranged below the liquid storage tanks, the liquid storage tanks are connected with the liquid inlet pipeline and the liquid discharge pipeline through a communicating pipe with an electromagnetic valve, the liquid inlet pipeline and the liquid discharge pipeline are connected with the tissue separator through a liquid pump, and the motor, the electromagnetic valve and the liquid pump are all electrically connected with the controller. The mesenchymal stem cell separation device is compact in structure, and after an operation program is set by the controller, the tissue block to be extracted only needs to be placed in the tissue separator, so that the device can automatically cut and extract the tissue block in a sample adding manner, and can automatically clean and sterilize the device after extraction is finished, thereby saving manpower and improving the separation efficiency of mesenchymal stem cells.

Description

An automatic mesenchymal stem cell separation device

technical field

The invention relates to the technical field of regenerative medicine experimental equipment, in particular to an automatic mesenchymal stem cell separation device.

Background technique

Stem cell research and regenerative medicine research have become the frontier and hotspot of today's biomedical research. Adult stem cells are undifferentiated stem cells that exist in adult tissues. They can differentiate into specific cell types, repair tissues and maintain tissue integrity. Therefore, they are widely used in the fields of regenerative biology and regenerative medicine. Adult stem cells with a wide range of applications in the human body include hematopoietic stem cells and mesenchymal stem cells. Among them, mesenchymal stem cells are mainly derived from bone marrow, viviparous tissues (such as umbilical cord and placenta) and fat. However, mesenchymal stem cells derived from viviparous tissue have been increasingly used in recent years due to the simplicity of extraction.

At present, in the field of basic research and clinical application research, mesenchymal stem cells are mainly obtained by cutting the viviparous tissue with manual scissors, so as to separate the internal stem cells. This method consumes a lot of time and manpower, and repeated shearing of the tissue during the operation will cause mechanical damage to the internal stem cells and reduce the separation efficiency of stem cells. There is still a lack of other isolation methods or instruments that can efficiently obtain stem cells.

Therefore, the existing technology still needs to be improved.

SUMMARY OF THE INVENTION

In view of the above-mentioned deficiencies of the prior art, the purpose of the present invention is to provide an automatic mesenchymal stem cell separation device, which aims to solve the problem that the existing mesenchymal stem cell separation device needs to consume a lot of time and manpower, and the stem cell separation efficiency is low. question.

The technical scheme of the present invention is as follows:

An automatic mesenchymal stem cell separation device, which comprises a housing, a controller arranged outside the housing, a tissue separator arranged inside the housing, arranged below the tissue separator and used to drive the Motor for tissue separator operation;

A number of liquid storage tanks are formed between the tissue separator and the shell through the partition plate, and a liquid inlet pipeline and a liquid discharge pipeline are arranged under the liquid storage tank, and a communication pipe and a liquid storage tank are arranged in the liquid storage tank. The liquid inlet pipe is connected with the liquid discharge pipe, and a solenoid valve is arranged on the communication pipe;

The liquid inlet pipeline and the liquid discharge pipeline are both connected with a liquid pump, and the liquid pump is connected with the tissue separator; the motor, the solenoid valve and the liquid pump are all electrically connected to the controller. connect.

In the automatic mesenchymal stem cell separation device, a liquid storage bag is arranged in the liquid storage tank, and the liquid storage bag and the communication pipe are connected by a valve joint.

The automatic mesenchymal stem cell separation device, wherein the valve joint includes a pressure cap assembly and an elastic interface assembly;

The pressing cap assembly includes a locking nut and a jacking pipe arranged on the locking nut;

The elastic interface assembly includes a valve body, a support base plate arranged in the valve body, a column arranged on the support base plate, and a spring and a ring plate sleeved on the column in sequence. There are through holes for liquid to flow through.

The automatic mesenchymal stem cell separation device, wherein the locking nut is slidably arranged on the top tube, and the top tube is provided with a hanging ear and a sealing ring for limiting the sliding range of the locking nut .

The automatic mesenchymal stem cell separation device, wherein the tissue separator comprises a cylindrical body, a central shaft arranged in the cylindrical body, a cutting assembly that is screwed on the central shaft and can move up and down, and is fixed. The stirring impeller is connected to the bottom end of the central shaft, and the cylinder cover is arranged on the top of the cylinder body.

The automatic mesenchymal stem cell separation device, wherein the central axis is a flat axis with a symmetrical cutting surface and a symmetrical circular arc surface, the cutting assembly includes a blade and a knife net, and the blade is installed in the center The symmetrical cutting surface of the shaft can slide up and down, the knife net is arranged below the blade and is threadedly connected with the symmetrical circular arc surface of the central axis, the central connecting part of the blade is provided with a hook, the knife A hook disc is correspondingly arranged on the central connecting part of the net, and the blade and the knife net are hooked and connected.

In the automatic mesenchymal stem cell separation device, the edge of the knife net is provided with a slider, and the inner wall of the cylinder is provided with a chute adapted to the slider.

In the automatic mesenchymal stem cell separation device, the motor is connected with the central shaft of the tissue separator through a transmission gear set.

In the automatic mesenchymal stem cell separation device, a battery module for powering the motor, the solenoid valve, the liquid pump and the controller is provided below the liquid storage tank.

The automatic mesenchymal stem cell separation device further includes a cart that facilitates the movement of the device.

Beneficial effects: The present invention provides an automatic mesenchymal stem cell separation device, comprising a housing, a controller arranged outside the housing, a tissue separator arranged inside the housing, a motor for driving the tissue separator to operate, A number of liquid storage tanks are formed between the separator and the shell, and the liquid inlet pipeline and the liquid discharge pipeline are arranged below the liquid storage tank. The liquid pipeline and the liquid discharge pipeline are connected with the tissue separator through the liquid pump, and the motor, the electromagnetic valve and the liquid pump are all electrically connected with the controller. The device for separating mesenchymal stem cells of the present invention has a compact structure. After the operation program is set by the controller, the device only needs to put the tissue block to be extracted into the tissue separator, and the device can automatically cut the tissue block and add samples to extract. , and after the extraction, the device can also be automatically cleaned and sterilized, which saves manpower and improves the separation efficiency of mesenchymal stem cells.

Description of drawings

1 is a schematic diagram of the assembly structure of an automatic mesenchymal stem cell separation device of the present invention;

2 is a schematic diagram of an explosion structure of an automatic mesenchymal stem cell separation device of the present invention;

Fig. 3 is the structural representation of a kind of valve joint of the present invention;

Fig. 4 is the exploded structure schematic diagram of a kind of tissue separator of the present invention;

5 is a schematic diagram of the internal structure of a tissue separator of the present invention;

6 is a schematic diagram of the connection structure of a blade and a blade net of the present invention;

The symbols in the attached drawings are as follows:

10-shell; 20-controller; 30-tissue separator; 40-motor; 50-partition; 60-liquid storage tank; 70-liquid inlet pipe; 80-discharge pipe; 90-connecting pipe; 100-liquid Pump; 110-lock nut; 120-top pipe; 130-valve body; 140-support bottom plate; 150-column; 160-spring; 170-ring piece; 180-through hole; 190-hanging ear; 200-seal Circle; 210-Cylinder; 220-Central Shaft; 230-Cutting Assembly; 240-Mixing Impeller; 250-Cylinder Cover; 260-Symmetrical Cutting Surface; 270-Symmetrical Arc Surface; 280-Blade; 290-Knife Net; 300 - Hook; 310 - Hook Disc; 320 - Slider; 330 - Chute; 340 - Transmission Gear Set; 350 - Battery Module; 360 - Cart.

Detailed ways

The present invention provides an automatic mesenchymal stem cell separation device. In order to make the purpose, technical solution and effect of the present invention clearer and clearer, the present invention is further described below in detail. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention.

Referring to FIG. 1 and FIG. 2 , an automatic mesenchymal stem cell separation device provided by the present invention includes a housing 10 , a controller 20 arranged outside the housing 10 , and a tissue separator 30 arranged inside the housing 10 . , a motor 40 arranged below the tissue separator 30 and used to drive the tissue separator to run;

A plurality of liquid storage tanks 60 are formed between the tissue separator 30 and the housing 10 through the partition plate 50 , and a liquid inlet pipe 70 and a liquid discharge pipe 80 are arranged below the liquid storage tank 60 . A communication pipe 90 is provided in the tank 60 to be connected with the liquid inlet pipe 70 and the liquid discharge pipe 80, and a solenoid valve is arranged on the communication pipe 90;

The liquid inlet pipe 70 and the liquid discharge pipe 80 are both connected to a liquid pump 100, and the liquid pump 100 is connected to the tissue separator 30; the motor 40, the solenoid valve and the liquid pump 100 Both are electrically connected to the controller 20 .

In terms of structure as a whole, the automatic mesenchymal stem cell separation device of the present invention mainly includes a control system (including a controller), a separation system (including a tissue separator and a motor), a sample adding and cleaning system (including a liquid storage tank, electromagnetic valve, liquid inlet pipe, liquid discharge pipe and liquid pump). After the mesenchymal stem cell separation device of the present invention has set the operation program through the controller, the tissue block to be extracted is placed in the tissue separator, and alcohol and PBS buffer are sequentially injected through the sample adding system to sterilize and wash the tissue block, and then the tissue block is sterilized and washed. After the separation system is used to cut the tissue quickly and accurately, the tissue block is enzymatically hydrolyzed under the action of the enzyme solution to complete the efficient extraction of mesenchymal stem cells. After the extraction, the pipeline and tissue separator can also be cleaned by the cleaning system. After cleaning and sterilization, the mesenchymal stem cell separation device of the present invention can automatically, efficiently and rapidly separate mesenchymal stem cells, reduce cell damage, reduce the risk of contamination, and improve stem cell acquisition efficiency and work efficiency.

In some embodiments, the control system in this technical solution also has the function of automatically checking the running status of each component, and when a component is abnormal, it will prompt which component is running abnormally (for example, power failure caused by battery damage, pipeline failure, etc.). liquid, the gear cannot run, etc.), the prompt can be displayed directly on the operation screen of the controller, or can be broadcast by voice, so that the degree of automation of the device is higher, and the follow-up maintenance test is simpler and more convenient. In the application scenario, before the sample is put in, the device needs to automatically and preliminarily check whether the system is running normally, that is, the device needs to be run in an empty run. When it is confirmed that all the components in the device can operate normally, it will prompt to put the sample, which can be avoided to a large extent. The device does not operate after the sample is placed, resulting in waste.

In a specific implementation, one or more communication pipes 90 in each liquid storage tank 60 may be provided. When there is one communication pipe 90, the liquid inlet and discharge of the liquid storage tank 60 share one communication pipe. The pipe has three ports; when two are provided, one of them is connected to the liquid inlet pipe 70 and the other one is connected to the liquid discharge pipe 80, and each connecting pipe is provided with a solenoid valve, and so on.

During specific implementation, one or more liquid pumps 100 may also be provided. When one liquid pump 100 is provided, the liquid pump 100 is a double-in and double-outlet pump; when two liquid pumps 100 are provided, One of the liquid pumps 100 is connected to the liquid inlet pipe 70 , the other liquid pump 100 is connected to the liquid discharge pipe 80 , and both liquid pumps 100 are electrically connected to the controller 20 .

In a specific implementation, four liquid storage tanks 60 may be provided, the liquid inlet pipeline 70 and the liquid discharge pipeline 80 may be set to be circular, and the liquid inlet pipeline 70 and the liquid drain pipeline 80 pass under the four liquid storage tanks 60 .

In a specific embodiment, the liquid storage tanks 60 can directly store liquid, and the liquid stored in each liquid storage tank 60 can be the same or different. In a specific case, one liquid storage tank is used for For alcohol storage, 2 reservoirs are used to store PBS buffer, and the other reservoir is used to store digestive enzyme solution.

In another specific embodiment, the liquid storage tank 60 is provided with a liquid storage bag, and the liquid storage bag and the communication pipe 90 are connected through a valve joint. In this embodiment, a liquid storage bag is provided in the liquid storage tank 60 to store the liquid, which on the one hand facilitates the storage of the liquid, avoids volatilization of the liquid and facilitates disassembly and assembly, and on the other hand, it can also prevent the liquid from affecting the liquid storage. Corrosion of the tank, preventing liquid contamination and reducing the structural strength of the device.

Referring to FIG. 3, in a specific embodiment, the valve joint includes a pressure cap assembly and an elastic interface assembly;

The pressing cap assembly includes a locking nut 110 and a top tube 120 arranged on the locking nut 110;

The elastic interface assembly includes a valve body 130 , a support base plate 140 disposed in the valve body 130 , a column 150 disposed on the support base plate 140 , and a spring 160 and a circular spring sleeved on the column 150 in sequence. The ring piece 170, the bottom of the upright post 150 is provided with a through hole 180 for liquid to flow through. Specifically, the two ends of the spring 160 are fixedly connected to the annular sheet 170 and the support base plate 140 respectively. In the initial state, the surface of the annular sheet 170 is flush with the surface of the valve body 130 and the surface of the column 150 .

In a specific embodiment, the lock nut 110 can be fixedly connected with the top pipe 120. In another specific embodiment, the lock nut 110 is slidably arranged on the top pipe 120, and the top pipe The tube 120 is provided with a mounting lug 190 and a sealing ring 200 for limiting the sliding range of the locking nut 110 .

In this embodiment, when the valve joint is connected, the locking nut 110 and the valve body 130 are screwed and fixed first, and then the jacking pipe 120 is pressed down. After 170, since the outer diameter of the jacking pipe 120 is smaller than the inner diameter of the valve body 130, and the inner diameter of the jacking pipe 120 is larger than the outer diameter of the upright column 150, the jacking pipe 120 can push the annular sheet 170 to move down, and at the same time, the sealing film at the port of the jacking pipe 120 is blocked. The upright post 150 is pierced, and then the jacking pipe 120 continues to move downward until the sealing ring 200 on the jacking pipe 120 is engaged in the gap between the locking nut 110 and the jacking pipe 120, and the locking of the jacking pipe 120 on the locking nut 110 is completed. After fixing, the fluid storage bag is connected to the communication pipe 90 .

Referring to FIG. 4 , in a specific embodiment, the tissue separator 30 includes a cylindrical body 210, and a central shaft 220 disposed in the cylindrical body 210 is screwed on the central shaft 220 and can move up and down The cutting assembly 230 , the stirring impeller 240 fixedly connected to the bottom end of the central shaft 220 , and the cylinder cover 250 arranged on the top of the cylinder body 210 . The function of the cylinder cover 250 is to cover the central shaft 220, the cutting assembly 230, and the stirring impeller 240 inside the cylinder body 210. The function of the stirring impeller 240 is to stir the tissue cut by the cutting assembly 230 to make it evenly mixed , the bottom end of the central shaft 220 is disposed through the bottom of the cylindrical body 210 , and the position where the central shaft 220 contacts the cylindrical body 210 is sealed.

Referring to FIG. 5 , in a specific embodiment, the central shaft 220 is a flat shaft with a symmetrical cutting surface 260 and a symmetrical circular arc surface 270 , and the cutting assembly 230 includes a blade 280 (including a central connecting portion and a cutting portion) ) and a knife net 290 (including a central connecting part and a filter screen part), the blade 280 is mounted on the symmetrical cutting surface 260 of the central shaft 220 and can slide up and down, and the knife net 290 is arranged below the blade 280 And it is threadedly connected with the symmetrical arc surface 270 of the central axis 220. Referring to FIG. 6, a hook 300 is provided on the central connecting portion of the blade 280, and a hook disc 310 is correspondingly provided on the central connecting portion of the knife net 290. , the blade 280 and the knife net 290 are hooked and connected. Specifically, two hook plates 310 can be arranged in parallel and spaced apart from each other. The shape of the hooks 300 is a "匚" shape. In the gap of the disk 310, two hooks 300 can be symmetrically arranged, and the blade 280 and the knife net 290 can be mutually rotated. Since the blade 280 is hooked and arranged on the blade 290, and the cutting part of the blade 280 is arranged obliquely, so that The cut tissue can quickly fall below the knife net 290 through the extrusion of the cutting portion of the blade 280, so as to avoid tissue cell damage caused by multiple cuts.

Referring to FIG. 5 , in a specific implementation manner, the edge of the knife net 290 is provided with a sliding block 320 , and the inner wall of the cylindrical body 210 is provided with a chute 330 adapted to the sliding block 320 . The function of the chute 330 is to restrict the knife net 290 to move up and down without rotating.

In a specific embodiment, the number of sliders 320 can be set to three, the number of sliding grooves 330 is correspondingly set to three, and the included angle between adjacent sliders 320 is 120°, and the number of sliders 320 is set to three The advantage is to make full use of the stability of the triangle, so that the knife net is more stable in the process of rising and falling.

Specifically, during the operation of the tissue separator of the present invention, with the rotation of the central shaft 220, the knife net 290 rises along the central shaft 220 without rotation due to the restriction of the chute 310 on the inner wall of the cylinder 210, and simultaneously pushes the blade 280 upward. When the knife net 290 and the blade 280 run to the unthreaded fault at the upper end of the central shaft 220, the knife net 290 stops running upward, but the blade 280 will continue to follow the central shaft 220 to rotate for a certain period of time until the top of the cylinder 210 is moved. The remaining tissue pieces are completely cut, and the blade 280 always follows the rotation of the central shaft 220 to cut the tissue pieces during the entire ascending process. The knife net 290 in the tissue separator of the present invention can move upward to compress the upper space of the cylinder body 210 to squeeze and fix the tissue block, so that the blade 280 can cut the tissue block more fully, and can adapt to the cutting and separation of different tissue block sizes. The cutting is more complete, and no manual adjustment is required, and the device is more convenient.

Referring to FIG. 2 , in a specific embodiment, the motor 40 is connected with the central shaft 220 of the tissue separator 30 through a transmission gear set 340 . The transmission gear group 340 is mainly composed of two gears with different diameters. The central shaft 220 is provided with a larger diameter gear, and the motor 40 is provided with a smaller diameter gear. The function of the transmission gear group is to change speed, and the transmission gear group can be fixed specifically. Set on the bracket below the reservoir 60 .

Referring to FIG. 2 , in a specific implementation manner, a battery module 350 for supplying power to the motor 40 , the solenoid valve, the liquid pump 100 and the controller 20 is provided below the liquid storage tank 60 . . Specifically, the battery module 350 can be provided with three pieces, which are specifically arranged in the space below the liquid pump 100 and the transmission gear set 340, so that the structure of the device is more compact, and the device is powered by the battery module 350 without connecting an external power source, and the device can be more compact. Flexible movement.

Referring to FIG. 1 , in a specific embodiment, the automatic mesenchymal stem cell separation device further includes a cart 360 that facilitates the movement of the device. In this embodiment, the housing 10 of the device is fixedly arranged on the cart 360, and the housing further includes a housing cover, which is used to seal the inside of the device.

In summary, the present invention provides an automatic mesenchymal stem cell separation device, comprising a casing, a controller disposed outside the casing, a tissue separator disposed inside the casing, and a motor for driving the tissue separator to operate, A number of liquid storage tanks are formed between the tissue separator and the shell. The liquid storage tank is provided with a liquid inlet pipeline and a liquid discharge pipeline. The liquid storage tank is connected with the liquid inlet pipeline and the liquid drain pipeline through a communication pipe with a solenoid valve. , the liquid inlet pipeline and the liquid discharge pipeline are connected with the tissue separator through the liquid pump, and the motor, the solenoid valve and the liquid pump are all electrically connected with the controller. The device for separating mesenchymal stem cells of the present invention has a compact structure. After the operation program is set by the controller, the device only needs to put the tissue block to be extracted into the tissue separator, and the device can automatically cut the tissue block and add samples to extract. , and after the extraction, the device can also be automatically cleaned and sterilized, which saves manpower and improves the separation efficiency of mesenchymal stem cells.

It should be understood that the application of the present invention is not limited to the above examples. For those of ordinary skill in the art, improvements or transformations can be made according to the above descriptions, and all these improvements and transformations should belong to the protection scope of the appended claims of the present invention.

Claims (10)

1. An automatic type mesenchymal stem cell separation device is characterized in that, comprising a casing, a controller arranged on the outside of the casing, a tissue separator arranged on the inside of the casing, arranged below the tissue separator and used a motor for driving the tissue separator; A number of liquid storage tanks are formed between the tissue separator and the shell through the partition plate, and a liquid inlet pipeline and a liquid discharge pipeline are arranged under the liquid storage tank, and a communication pipe and a liquid storage tank are arranged in the liquid storage tank. The liquid inlet pipe is connected with the liquid discharge pipe, and a solenoid valve is arranged on the communication pipe; The liquid inlet pipeline and the liquid discharge pipeline are both connected with a liquid pump, and the liquid pump is connected with the tissue separator; the motor, the solenoid valve and the liquid pump are all electrically connected to the controller. connect. 2 . The automatic mesenchymal stem cell separation device according to claim 1 , wherein a liquid storage bag is provided in the liquid storage tank, and the liquid storage bag and the communication pipe are connected through a valve joint. 3 . . 3. The automatic mesenchymal stem cell separation device according to claim 2, wherein the valve joint comprises a pressure cap assembly and an elastic interface assembly; The pressing cap assembly includes a locking nut and a jacking pipe arranged on the locking nut; The elastic interface assembly includes a valve body, a support base plate arranged in the valve body, a column arranged on the support base plate, and a spring and a ring plate sleeved on the column in sequence. There are through holes for liquid to flow through. 4 . The automatic mesenchymal stem cell separation device according to claim 3 , wherein the locking nut is slidably arranged on the top tube, and the top tube is provided with a locking nut for restricting the Mounting lugs and seals for sliding range. 5 . The automatic mesenchymal stem cell separation device according to claim 1 , wherein the tissue separator comprises a cylindrical body, and a central shaft arranged in the cylindrical body is screwed on the central shaft and is threaded. 6 . A cutting assembly that can move up and down, a stirring impeller fixedly connected to the bottom end of the central shaft, and a cylinder cover arranged on the top of the cylinder body. 6. The automatic mesenchymal stem cell separation device according to claim 5, wherein the central axis is a flat axis with a symmetrical cutting surface and a symmetrical circular arc surface, and the cutting assembly comprises a blade and a knife net, The blade is installed on the symmetrical cutting surface of the central axis and can slide up and down, the knife net is arranged below the blade and is threadedly connected with the symmetrical circular arc surface of the central axis, and the central connecting part of the blade A hook is provided on the knife net, a hook disk is correspondingly arranged on the central connecting part of the knife net, and the blade and the knife net are hooked and connected. 7 . The automatic mesenchymal stem cell separation device according to claim 6 , wherein the edge of the knife net is provided with a sliding block, and the inner wall of the cylinder is provided with a sliding block adapted to the sliding block. 8 . Chute. 8 . The automatic mesenchymal stem cell separation device according to claim 1 or 2 , wherein the motor is connected with the central shaft of the tissue separator through a transmission gear set. 9 . 9 . The automatic mesenchymal stem cell separation device according to claim 1 or 2, characterized in that, a device for the motor, the solenoid valve, the liquid pump and the Controller powered battery module. 10 . The automatic mesenchymal stem cell separation device according to claim 1 or 2 , further comprising a cart that facilitates the movement of the device. 11 .

Images