CN111557447A

CN111557447A - Preparation of dendrobium officinale polysaccharide buccal tablet and application of dendrobium officinale polysaccharide buccal tablet in immune enhancement

Info

Publication number: CN111557447A
Application number: CN202010585113.8A
Authority: CN
Inventors: 丁华敏
Original assignee: SHANGHAI PUNAN HOSPITAL
Current assignee: SHANGHAI PUNAN HOSPITAL
Priority date: 2020-06-24
Filing date: 2020-06-24
Publication date: 2020-08-21

Abstract

The invention discloses a preparation method of a dendrobium officinale polysaccharide buccal tablet and application of the dendrobium officinale polysaccharide buccal tablet in an immunity enhancing effect. The total extraction process of the polysaccharide comprises the following steps: decocting herba Dendrobii coarse powder with 40 times of water for 2 hr, filtering, concentrating the filtrate to 0.2g crude drug/ml, and precipitating with 5 times of ethanol to obtain Dendrobium candidum polysaccharide powder. The dendrobe polysaccharide powder is mixed with mannitol, aspartame, magnesium stearate, microcrystalline cellulose, Mentholum, and polyvidone to make into buccal tablet. The buccal tablet can improve yin deficiency symptoms of yin deficiency mice model caused by thyroid hormone. Improvements in or relating to⁶⁰S180 tumor bearing caused by Co radiotherapyMice have a reduction in WBCs in peripheral blood and an increase in the number of bone marrow nucleated cells. Has effects in improving activity, resisting oxidative damage, and enhancing immunity. The dendrobium officinale polysaccharide buccal tablet prepared by the optimal dendrobium polysaccharide extraction process has an obvious immunity enhancing effect and can be used in the fields of immunity enhancing medicines, health products and foods.

Description

Preparation of dendrobium officinale polysaccharide buccal tablet and application of dendrobium officinale polysaccharide buccal tablet in immune enhancement

Technical Field

The invention relates to the technical field of medicines, foods and health-care products, in particular to preparation of a dendrobium officinale polysaccharide buccal tablet and application of the dendrobium officinale polysaccharide buccal tablet in an immunity enhancing effect.

Background

Immunity is the body's own defense mechanism, and is the body's ability to recognize and destroy any foreign body (virus, bacteria, etc.) that invades from the outside, to treat aged, damaged, dead, denatured self cells, and to recognize and treat mutant cells and virus-infected cells in the body. The immunity characteristics of the newborn usually show that the immune system is not mature enough, the function is not perfect, and the newborn does not contact various pathogens of the extrauterine environment and various antigenic substances such as food protein and the like. The pregnancy is affected by the maternal antibodies that pass through the placenta, leaving the newborn with physiological hypoimmunity. At present, a plurality of ways and methods for improving the immunity are available, the sepiolite is simple and effective, zinc supplementation is paid more attention at ordinary times, foods rich in zinc such as walnuts, egg yolks, fresh oysters and marine products are eaten more in daily diet, and some proteinzinc can be eaten, so that the immunity improvement is helped to a certain extent.

Modern immunology considers that immunity is the physiological response of the human body to recognize and eliminate "isohexia". The immune system performs this function in the human body. For millions of years, human lives in an environment which is suitable for living and full of danger, so that the human can survive and also obtains extraordinary immunity. Immunity is therefore said to be a product of the biological evolution process. Immunity can be divided into non-specific immunity and specific immunity. The immunity can be divided into the following according to the different obtaining modes: 1. innate immunity is inherent in a person's life. For example, swine fever spreads rapidly in swine herds, but is unrelated to humans. This is because humans are not naturally afflicted with this disease; 2. acquired immunity is acquired naturally in the life process after the human beings come down, or passively by an artificial assistance method.

The lack of endogenous peptide in human body can cause low immune function, the body presents an intolerant state due to the low immune function, cold viruses enter in a deficiency mode and are infected concurrently, particularly the infection concurrent with influenza has serious illness and high death rate. With the development of modern immunology, people gradually and fully know the relationship between peptides and immunity. Insufficient peptides in the body can cause immune organ hypoplasia, atrophy, cellular immunity,Humoral immunityComplement function and phagocytosis have important effects.

The immune system cannot normally exert protective effect due to various reasons, and in this case, the immune system is very easy to infect bacteria, viruses, fungi and the like, so the most direct expression of low immunity is easy to cause diseases. The frequent illness aggravates the consumption of the organism, so the general manifestations of weak constitution, malnutrition, listlessness, fatigue, weakness, appetite reduction, sleep disorder and the like exist, and the patients with illness, injection and medication become the frequent meals of the home. Recovery from each illness can take a long time and often attacks repeatedly. This causes physical and intellectual development failure and also induces serious diseases.

The deep cause is hypoimmunity or poor immunity. When the human body is immune dysfunctional or the immune system is not healthy, the following problems are repeatedly caused: the cold attacks repeatedly, the tonsillitis attacks repeatedly, the asthma attacks repeatedly, the bronchitis attacks repeatedly, the pneumonia attacks repeatedly, and the diarrhea attacks repeatedly … …, so that ten million people cannot see the small sight.

Dendrobium officinale (with the scientific name: Dendrobium officinale Kimura et Migo) belonging to the genus Dendrobium of the family OrchidaceaeHerbal medicinePlants, which prefer to grow in warm, humid, semi-yin and semi-yang environments, are distributed in Anhui province,Fujian tea、Zhejiang river、Guangxi province、Cloud South ChinaAnd so on. Described at the earliest inShennong herbal channelThe angle of the attack of infection is the head of Jiudao Xiancao in China, and herbs in the past generations of China are all collected and carried. The property and taste effects are as follows: sweet in flavor and slightly cold in nature. Promoting the production of body fluid and nourishing the stomach; nourishing yin and clearing heat; moistening lung and invigorating kidney; improving eyesight and strengthening waist. The international medicinal plant world calls it as "panda of medicine world"; is called as 'life saving grass for the nation's life. Modern researches show that the dendrobium can improve the immunity of human bodies, has the effects of resisting aging and tonifying consumptive diseases of five internal organs.

The dendrobium has various varieties, and the medicinal varieties collected by Chinese pharmacopoeia comprise dendrobium nobile, dendrobium fimbriatum, dendrobium chrysotoxum, dendrobium candidum and the like. All the time, doctors think that the dendrobium officinale is the top-quality product and is most advocated, and in reality, the dendrobium officinale is the most popular among consumers because of the largest sales volume and the most expensive price.

At present, the clinical application of dendrobium officinale mainly comprises the following steps: fresh, rolled into Dendrobium candicum, and made into oral liquid. These methods all have certain disadvantages to varying degrees. Fresh use: affected by seasons, the fertilizer can not be used in non-harvesting seasons; moreover, the fresh dendrobium officinale has large dryness and has side effects of causing diarrhea and the like; rolling into dendrobium candicum: the processing cost is high, and sometimes, the labor cost exceeds the purchase cost of the medicinal materials; moreover, when the patient takes the medicine, the medicine needs to be cooked for a long time (2 hours), and the use is extremely inconvenient; preparing dendrobium officinale oral liquid: the traditional Chinese medicine composition is convenient to take, but has poor stability, and the effectiveness of the traditional Chinese medicine composition is seriously influenced. Particularly, in the dendrobium products, the active ingredients are mainly polysaccharides, the storage stability is poor in an aqueous solution state, and the active ingredients are easy to separate out, so that the effectiveness is reduced. Moreover, in order to keep the preparation without mildewing, a certain amount of preservative is required to be added into the preparation, so the safety of the preparation is influenced to a certain extent.

In view of the above, there is a need to develop a dendrobium officinale polysaccharide buccal tablet which is convenient to carry, has high stability and good effectiveness, and can be used in a medicament for enhancing human immunity.

Disclosure of Invention

The invention aims to provide a dendrobium officinale polysaccharide buccal tablet, a preparation method thereof and an immunity enhancing effect.

In order to achieve the purpose, the technical scheme adopted by the invention is as follows:

the invention provides a dendrobium officinale polysaccharide buccal tablet, a preparation method and an immunity enhancing effect thereof.

The dendrobium officinale polysaccharide buccal tablet is prepared from the following raw materials: dendrobium officinale polysaccharide powder, a filler, a flavoring agent, a lubricant and a disintegrating agent.

The dendrobium officinale polysaccharide buccal tablet is prepared from the following raw materials: dendrobium officinale polysaccharide powder, mannitol, aspartame, microcrystalline cellulose, menthol, povidone and magnesium stearate.

Composition (I)	Content (%)
		Dendrobium officinale polysaccharide powder	20
Mannitol	58.5
		Microcrystalline cellulose	20
Povidone	1
		Menthol crystal	0.25
Aspartame	0.25
		Magnesium stearate (after finishing granules)	0.3 (percentage of the total weight)

The dendrobium officinale polysaccharide buccal tablet has high bioavailability and good taste, and can be dissolved within half an hour.

The preparation method of the dendrobium officinale polysaccharide powder comprises the following steps: taking dendrobium officinale coarse powder, adding 40 times of water by volume, extracting for 120min under the condition of slight boiling, filtering, concentrating the filtrate to 0.2g of crude drug/ml, then adding ethanol with the volume 5 times of that of the concentrated solution, standing overnight, filtering out precipitates, washing with a small amount of ethanol for 2-3 times, vacuum-drying at 70 ℃, crushing into fine powder, and sieving with a 60-mesh sieve to obtain the dendrobium officinale polysaccharide powder.

The preparation method of the polysaccharide buccal tablet comprises the following steps: taking dendrobium polysaccharide superfine powder, mannitol, aspartame, microcrystalline cellulose, menthol and povidone; and (3) granulating by using a wet method under the formula, drying at 60 ℃, granulating, adding magnesium stearate, uniformly mixing, and tabletting to obtain the dendrobium officinale polysaccharide buccal tablet.

Due to the adoption of the technical scheme, the invention has the following advantages and beneficial effects:

according to the dendrobium nobile buccal tablet, the dendrobium nobile total polysaccharide components are extracted to prepare the dendrobium nobile buccal tablet, the thyroid tablet is adopted to lavage stomach to replicate a yin deficiency model mouse, and whether the dendrobium nobile buccal tablet can improve the yin deficiency symptom of the mouse or not is researched, so that the activity ability and the antioxidant injury of an organism are improved. Establishing S180 tumor-bearing mouse model, observing the dendrobium officinale polysaccharide buccal tablet pair⁶⁰Co irradiation of S180 tumor-bearing mice has synergistic and attenuated effects. The experimental results show that: the dendrobium officinale polysaccharide buccal tablet can improve the symptom of yin deficiency of mice, obviously reduce the times of autonomous activities of the mice with yin deficiency and reduce the content of serum MDA. The dendrobium officinale polysaccharide buccal tablets have a treatment effect on mice of a yin deficiency model caused by thyroid hormone, can improve the yin deficiency symptoms of the mice, improve the activity of organisms and resist oxidative damage. Compared with the group consisting of tumor bearing tablets, radiotherapy tablets and dendrobium officinale polysaccharide buccal tablets⁶⁰The reduction of WBC (white blood cell count) in peripheral blood of S180 tumor-bearing mice caused by Co radiotherapy has obvious improvement effect, and the number of marrow nucleated cells is increased; obviously reduces the tumor weight of S180 sarcoma mice, improves the tumor inhibition rate, has obvious tumor inhibition and synergism on S180 tumor of mice, and can reduce⁶⁰Toxic and side effects caused by Co irradiation. Can be used for preparing medicine, health product or food for enhancing immunity.

According to the invention, an optimal dendrobium polysaccharide extraction process is screened out through a large number of experiments, the prepared compound dendrobium officinale tablet has an obvious immunity enhancement effect, the whole process is reasonable in design, the operability is strong, and the industrial production can be realized.

Detailed Description

In order to more clearly illustrate the invention, the invention is further described below in connection with preferred embodiments. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the invention.

Example 1

Preparation process of dendrobium officinale polysaccharide powder

1. Design of orthogonal experiments

According to the water-soluble property of polysaccharide, reflux heating extraction method is adopted, three test factors of medicinal material granularity, liquid-material ratio and heating time are selected, three levels are selected from each factor, and L9 (3) is utilized⁴) The table shows that the factors and the levels are arranged horizontally, and the dendrobium officinale is extracted by using the listed conditions, and the specific factors and the levels are shown in table 1.

TABLE 1 factors for orthogonal experimental investigation

2. Investigation of concentration multiple and alcohol precipitation concentration

After the dendrobium officinale polysaccharide extracting solution is obtained, the extracting solution is concentrated to a certain concentration, and then ethanol is added to precipitate the polysaccharide.

(1) Concentrating by taking 1g of crude powder of the medicinal materials, adding 40 times of water, heating, refluxing and extracting for 120min for once, concentrating the obtained extracting solution to be solutions of 0.05g of crude drug/ml, 0.1g of crude drug/ml and 0.2g of crude drug/ml respectively, adding the dendrobium officinale polysaccharide with ethanol of 4 times of the volume of the concentrated solution, drying in vacuum, weighing the obtained polysaccharide weight, and carrying out three times of experiments in each group.

(2) Alcohol precipitation concentration is to take 1g of crude drug powder, add 40 times of water, heat and reflux to extract for one time for 120min, respectively concentrate the obtained extract to a solution containing 0.1g of crude drug/ml, add 2, 3, 4 and 5 times of the volume of the concentrated solution of the dendrobium officinale polysaccharide, dry in vacuum, weigh the obtained polysaccharide weight, and carry out three times of each group of experiments.

3. Results of the experiment

TABLE 2 visual analysis of orthogonal test results

As can be seen from Table 2, the order of the influence of the particle size, the liquid-material ratio, the extraction time and the extraction frequency on the extraction amount of the dendrobium officinale polysaccharide is as follows: the granularity, the extraction time, the liquid-material ratio and the extraction frequency are determined, the optimal extraction condition of the dendrobium officinale polysaccharide is obtained by adding 40 times of water volume to the dendrobium officinale coarse powder, extracting for 120min under the condition of slight boiling, and extracting once.

TABLE 3 concentration factor investigation

TABLE 4 alcohol precipitation ratio investigation

According to the data, the dendrobium officinale polysaccharide is concentrated to the crude drug content of 0.05g/ml, the concentrated solution is mixed with 5 times of ethanol, and the maximum dendrobium officinale polysaccharide is obtained. However, considering the consumption of ethanol in the extraction process and the little difference between the yields of the dendrobium officinale polysaccharide concentrated by 0.05g/ml and 0.2g/ml, the dendrobium officinale polysaccharide is concentrated to a concentration of 0.2g/ml containing crude drug.

In summary, the total extraction process of the dendrobium officinale polysaccharide comprises the following steps: adding 40-fold volume of water into the dendrobium officinale coarse powder, extracting for 120min under the condition of slight boiling, filtering, concentrating the filtrate to 0.2g of crude drug/ml, then adding ethanol with the volume 5 times that of the concentrated solution, standing overnight, filtering out precipitates, washing for 2-3 times with a small amount of ethanol, and drying in vacuum to obtain the dendrobium officinale coarse powder.

Example 2

Process for preparing dendrobium officinale polysaccharide buccal tablet

1. Selection of the Filler

TABLE 5

The content of the dendrobium powder in the prescription is not too high due to the high viscosity of the dendrobium polysaccharide powder, otherwise, the hardness of the tablet is too high and the tablet is not easy to contain. Mannitol is easy to absorb moisture, can promote the melting of the buccal tablet, and can improve the taste of the buccal tablet.

2. Screening of corrective Agents

TABLE 6

As a buccal tablet, certain sweetness is required, and 0.25% of aspartame has good sweetness; because microcrystalline cellulose is added, the buccal tablet can feel powdery after being melted, and the addition of menthol is helpful for relieving the taste.

3. Selection of lubricants

TABLE 7

Magnesium stearate is a hydrophobic lubricant, is easy to be mixed with the granules uniformly, has smooth and beautiful tablet surface after tabletting and is most widely applied. Therefore, magnesium stearate is selected as the lubricant in the invention. 0.3 percent of the total weight of the composition can make the tablet smooth and complete.

4. Selection of disintegrating agent

TABLE 8

Amount of Povidone (%)	Tablet Properties
		0.5	The buccal tablet is not easy to be dissolved, and needs more than half an hour
1	The tablet can be dissolved in water for about 15min
		2	The buccal tablet is loose and has poor taste

Because the dendrobium polysaccharide powder has high viscosity and is not easy to disintegrate in tabletting, the common povidone is selected as the disintegrant. 1% of povidone has good disintegrating effect.

In summary, the formula process of the dendrobium officinale polysaccharide buccal tablet comprises the following steps: dendrobium officinale polysaccharide powder: mannitol: microcrystalline cellulose: polyvidone: aspartame: mixing Mentholum (20:58.5:20:1:0.25:0.25), wetting with 50% ethanol, granulating with 18 mesh sieve, oven drying at 60 deg.C, sieving with 18 mesh sieve, grading, adding 0.3% magnesium stearate, mixing, and tabletting.

Example 3

Experiment for Immunopotentiating Effect

1. Influence of dendrobium officinale polysaccharide buccal tablets on yin deficiency model mice

The dendrobium nobile buccal tablets are prepared by extracting the total polysaccharide components of dendrobium nobile, are applied to gastric lavage to replicate a yin deficiency model mouse, and are used for researching whether the dendrobium nobile buccal tablets can improve the yin deficiency symptoms of the mouse and improve the activity and the oxidation damage resistance of an organism.

The experimental results are as follows:

1) general State Observation

From day 5, the mice in the yin-deficiency model group had sparse hair compared with the normal control group, had thinner subcutaneous fat, had darker soles (reddish) than the normal control group, had dry stools and yellow urine. By the 10 th day, the mice in the yin deficiency model group grew slowly, had poor skin and hair glossiness, were easily irritated, had frequent activities, and had significantly increased body temperature, indicating that the yin deficiency model was successfully made. Compared with the model group, the dendrobium officinale polysaccharide buccal tablets have the advantages that the hair color of mice in the low, medium and high dose groups and the pulse-activating decoction group is relatively smooth and moist, the body shape is large, the color of soles is light red, and the defecation is relatively normal.

2) Influence on free Activity of mice

Compared with a normal control group, the times of the autonomic activity of the mice in the yin deficiency model group are obviously increased, and the significant difference is realized (P < 0.01). After the medicine is taken for prognosis, compared with a model group, the activity times of mice in the dendrobium officinale polysaccharide buccal tablets and in a high-dose group are obviously reduced, and the difference is obvious (P is less than 0.05); the activity frequency of the dendrobium officinale polysaccharide buccal tablet in a low-dose group is reduced, but the dendrobium officinale polysaccharide buccal tablet has no statistical significance (P is more than 0.05). The number of activities of the positive control pulse-activating decoction mice is obviously reduced compared with that of the model group (P < 0.05). The results are shown in Table 9.

TABLE 9 influence of Dendrobium officinale polysaccharide buccal tablet on free activity of mice: (

n＝10)

Note:^*P<0.05，^**P<0.01vs model set;^#P<0.05，^##P<0.01 vs. normal control group.

3) The content of malondialdehyde in mouse serum

As can be seen from the results in Table 3, compared with the normal control group, the serum MDA level of the mice in the yin deficiency model group is obviously increased and the difference is obvious (P < 0.01). Compared with a model group, the serum MDA content of mice in the dendrobium officinale polysaccharide buccal tablets and in a high-dose group is obviously reduced, and the significant difference is achieved (P is less than 0.05, and P is less than 0.01); the dendrobium officinale polysaccharide buccal tablet has the advantages that the serum MDA content of mice in a low-dose group is reduced, but the statistical significance is not achieved (P is more than 0.05). Compared with the serum MDA content of the mice in the model group, the positive control pulse-activating decoction group has the advantage that the serum MDA content is obviously reduced (P < 0.05).

TABLE 10 serum malondialdehyde changes in the groups of mice: (

n＝10)

The result shows that the dendrobium officinale polysaccharide buccal tablet can improve the symptom of yin deficiency of mice, obviously reduce the times of autonomous activities of the yin deficiency mice and reduce the content of serum MDA. The dendrobium officinale polysaccharide buccal tablets have a treatment effect on mice of a yin deficiency model caused by thyroid hormone, can improve the yin deficiency symptoms of the mice, improve the activity of organisms and resist oxidative damage.

2. Synergistic and attenuated effect of dendrobium officinale polysaccharide buccal tablet on radiotherapy of tumor-bearing animals

Establishing S180 tumor-bearing mouse model, except tumor-bearing control group, 6Gy once for 10 days after gastric lavage⁶⁰Co was irradiated systemically for 15 days. And blood was taken from the tail vein on days 3, 7, and 11 after irradiation, and peripheral blood cell counts were performed. Mice were sacrificed 24h after the last administration, the body weight and tumor weight were weighed, and the number of bone marrow nucleated cells and the like were counted. Pair for observing dendrobium officinale polysaccharide buccal tablets⁶⁰Co irradiation of S180 tumor-bearing mice has synergistic and attenuated effects.

The experimental results are as follows:

1) to tumor-bearing mice⁶⁰Effect of WBC in peripheral hemograms after Co radiotherapy

Tumor-bearing mice were compared to tumor-bearing control groups⁶⁰The WBC number in peripheral blood was significantly reduced (P) after Co radiation therapy<0.01) and continued until day 11 after radiotherapy. On the 3 rd day after the radiotherapy, compared with the lotus tumor + radiotherapy group, the low, medium and high dose groups of the lotus tumor + radiotherapy + dendrobium officinale polysaccharide buccal tablets and the positive control (pulse-activating decoction and hematopoiesis) group mice have no obvious difference in WBC number in peripheral blood. On the 7 th day after the radiotherapy, compared with the group of the tumor bearing and the radiotherapy, the WBC number in the peripheral blood of the tumor bearing, the radiotherapy and the dendrobium officinale polysaccharide buccal tablet and the peripheral blood of the high-dose group are obviously increased (P)<0.05，P<0.01); the WBC number in peripheral blood of the mice in the group consisting of the lotus tumor, the radiotherapy and the pulse-activating decoction is obviously increased (P) compared with the mice in the group consisting of the lotus tumor and the radiotherapy<0.05，P<0.01). On the 11 th day after the radiotherapy, compared with the group of the tumor bearing and the radiotherapy, the number of WBC in the peripheral blood of the group of the tumor bearing, the radiotherapy and the dendrobium officinale polysaccharide buccal tablet is obviously increased (P)<0.05，P<0.01); the WBC number in peripheral blood of the mice in the group consisting of the lotus tumor, the radiotherapy and the pulse-activating decoction is obviously increased (P) compared with the mice in the group consisting of the lotus tumor and the radiotherapy<0.05，P<0.01). The results are shown in Table 11.

TABLE 11 Effect of Dendrobium officinale polysaccharide buccal tablets on WBC in peripheral hemogram of tumor-bearing mice after radiotherapy ((

n＝10)

Note:^*P<0.05，^**P<0.01vs tumor bearing + radiotherapy group;^#P<0.05，^##P<0.01vs tumor-bearing control group.

2) Influence on the number of bone marrow nucleated cells after radiation therapy in tumor-bearing mice

Tumor-bearing mice were compared to tumor-bearing control groups⁶⁰After Co irradiation radiotherapy, the number of bone marrow nucleated cells is obviously reduced, and the difference is obvious (P)<0.01). Compared with the group of the tumor bearing and the radiotherapy, the bone marrow nucleated cells of the mice in the group of the tumor bearing, the radiotherapy and the dendrobium officinale polysaccharide buccal tablets with medium dose and high dose are obviously increased and have obvious difference (P)<0.05,P<0.01); the number of nucleated cells in bone marrow of mice in the low-dose group of the Holoma + radiotherapy + dendrobium officinale polysaccharide buccal tablets is increased, but the statistical significance (P) is not achieved>0.05). The bone marrow nucleated cell number of the mouse with the tumor and radiotherapy groups is obviously increased (P)<0.05). Experimental results show that the dendrobium officinale polysaccharide buccal tablets have a remarkable effect of increasing the number of bone marrow nucleated cells caused by radiotherapy. The results are shown in Table 12.

TABLE 12 influence of Dendrobium officinale polysaccharide buccal tablets on the number of bone marrow nucleated cells after radiotherapy of tumor-bearing mice: (

n＝10)

3) To is aligned with⁶⁰Effect of Co radiotherapy on inhibition of S180 sarcoma Activity

As can be seen from the results in Table 13, tumor-bearing mice were compared with the tumor-bearing control group⁶⁰After Co irradiation radiotherapy, the tumor weight (P) of S180 sarcoma mice is remarkably reduced<0.05). Compared with the group of the lotus tumor and the radiotherapy, the group of the lotus tumor, the radiotherapy and the dendrobium officinale polysaccharide buccal tablet with low dose, medium dose and high dose can reduce the tumor weight of the S180 sarcoma mouse, and the difference is very obvious (P)<0.05，P<0.01). The tumor inhibition rate of the tumor-bearing mice in the high-dose group of the tumor-bearing mice, namely the tumor-bearing mice, the radiotherapy and the dendrobium officinale polysaccharide buccal tablets is the highest and reaches 35.52%. The tumor weight of the mouse with the tumor, the radiotherapy and the hematopoiesis is reduced compared with that of the mouse with the tumor and the radiotherapy S180 sarcoma, but the statistical significance is not achieved (P)>0.05). The tumor weight of the mouse with the lotus tumor, the radiotherapy and the pulse-activating decoction is obviously reduced (P) compared with that of the mouse with the S180 sarcoma in the lotus tumor and radiotherapy groups<0.01). Experiments show that the dendrobium officinale polysaccharide buccal tablets have a synergistic effect on resisting mouse S180 sarcoma during radiotherapy.

TABLE 13 Dendrobium officinale polysaccharide buccal tablet pair⁶⁰Effect of Co radiotherapy on inhibition of S180 sarcoma Activity (

n＝10)

Dendrobium officinale polysaccharide buccal tablet pair⁶⁰The reduction of WBC (white blood cell count) in peripheral blood of S180 tumor-bearing mice caused by Co radiotherapy has obvious improvement effect, and the number of marrow nucleated cells is increased; obviously reduces the tumor weight of S180 sarcoma mice, improves the tumor inhibition rate, has obvious tumor inhibition and synergism on S180 tumor of mice, and can reduce⁶⁰Toxic and side effects caused by Co irradiation.

In view of the animal experiment evaluation results, the dendrobium officinale polysaccharide buccal tablet provided by the invention has a good immunity enhancing effect, and therefore, can be used for preparing immunity enhancing medicines, health products or foods.

The embodiments described above are intended to facilitate the understanding and appreciation of the application by those skilled in the art. It will be readily apparent to those skilled in the art that various modifications to these embodiments may be made, and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present application is not limited to the embodiments herein, and those skilled in the art who have the benefit of this disclosure will appreciate that many modifications and variations are possible within the scope of the present application without departing from the scope and spirit of the present application.

Claims

1. The dendrobium officinale polysaccharide buccal tablet is characterized by being prepared from the following raw materials: dendrobium officinale polysaccharide powder, a filler, a flavoring agent, a lubricant and a disintegrating agent.

2. The dendrobium officinale polysaccharide buccal tablet according to claim 1 is characterized by being prepared from the following raw materials: dendrobium officinale polysaccharide powder, mannitol, aspartame, microcrystalline cellulose, menthol, povidone and magnesium stearate;

and the contents of the components are as follows by weight percentage:

3. the dendrobium officinale polysaccharide buccal tablet according to claim 1 or 2, wherein the preparation method of the dendrobium officinale polysaccharide powder comprises the following steps:

(1) taking dendrobium officinale coarse powder, adding 40 times of water by volume, extracting for 120min under the condition of slight boiling, filtering, concentrating the filtrate to 0.2g of crude drug/ml, then adding ethanol with the volume 5 times of that of the concentrated solution, standing overnight, filtering out precipitates, washing with a small amount of ethanol for 2-3 times, vacuum-drying at 70 ℃, crushing into fine powder, and sieving with a 60-mesh sieve to obtain dendrobium officinale polysaccharide powder;

(2) taking dendrobium polysaccharide powder, mannitol, aspartame, microcrystalline cellulose, menthol and povidone; and (3) granulating by using a wet method under the formula, drying at 60 ℃, granulating, adding magnesium stearate, uniformly mixing, and tabletting to obtain the dendrobium officinale polysaccharide buccal tablet.

4. The application of the dendrobium officinale polysaccharide buccal tablet according to claim 1 in the aspect of immune enhancing activity is realized in the fields of medicines, health products and foods.

5. The dendrobium officinale polysaccharide powder of claim 3 is applied to preparation of buccal tablets, granules, oral mixtures, decocted extract, compressed sugar blocks and tea drinks.