CN111534511A - Reaction liquid for nucleic acid purification and recovery, nucleic acid recovery kit and application thereof - Google Patents
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Abstract
A reaction solution for nucleic acid purification and recovery, a nucleic acid recovery kit and application thereof, the reaction solution for nucleic acid purification and recovery comprises salts, a nucleic acid solid phase carrier and water; a nucleic acid recovery kit comprises the reaction solution for nucleic acid purification and recovery and a reagent container. A nucleic acid separation reagent comprising the reaction solution for nucleic acid purification and recovery and an alcohol; the reaction solution for nucleic acid purification and recovery is mixed with a biological sample containing nucleic acid, then alcohol is added, and under the condition of centrifugation, the nucleic acid can be separated from the biological sample. The reaction solution for nucleic acid purification and recovery is matched with alcohols, and the solubility of nucleic acid is changed through the alcohols, so that the nucleic acid is separated from a biological sample and is combined on a nucleic acid solid phase carrier, the nucleic acid is separated in a single step, the reaction solution for nucleic acid purification and recovery is suitable for purification and recovery of all types of nucleic acid, and the reaction solution has the advantages of simple process, low cost, energy conservation, high efficiency and low flux.
Description
Technical Field
The invention belongs to the technical field of nucleic acid purification and recycling, and particularly relates to a reaction liquid for nucleic acid purification and recycling, a nucleic acid recycling kit and application thereof.
Background
The technology for extracting and purifying nucleic acid molecules is a key technology for molecular biological research, and is a precondition technology for a series of molecular biological analyses such as nucleic acid amplification, DNA fragment connection, vector construction, high-throughput sequencing and the like. The pure nucleic acid sample extracted from the mixed experimental sample has very important significance for molecular biological research.
In the genetic studies, a step of recovering and purifying nucleic acids from a biological sample is first required. If nucleic acid can be recovered with high purity and high yield, then it becomes possible to perform highly sensitive gene detection in the subsequent detection reaction. Typical examples of the method for recovering nucleic acid include phenol/chloroform extraction, ethanol precipitation, column adsorption, and magnetic bead adsorption.
High purity and high concentration nucleic acid samples are always one of the important conditions for obtaining good experimental results in molecular biology experiments. Although there are many methods for preparing single-stranded DNA, double-stranded DNA and RNA, among them, main methods such as column recovery method, magnetic bead purification method; however, the column type recovery method has the defects of complicated operation, incomplete removal of fluorescent impurities, low recovery rate of nucleic acid fragments smaller than 200bp and the like; the magnetic bead purification method has the advantages of thorough purification, wide applicable nucleic acid types and high recovery rate, but has the defect of high price. Therefore, it is applicable to all types of nucleic acids, and a rapid, low-cost, and high-throughput method is not abundant.
As the demand for nucleic acid separation becomes more frequent, the sources of samples to be operated become more and more extensive, and thus, there is an urgent need to provide a purification method which can save the processing time and cost, and has a rapid purification and separation process and convenient operation. The present invention provides a rapid and convenient method and reagent (composition) for nucleic acid purification and recovery, which can eliminate the traditional complicated and time-consuming multi-step method for nucleic acid isolation and purification.
In addition, the invention aims at the problems that the existing imported magnetic bead purification kit is too expensive, the steps of a column type recovery method are more complicated, and the invention is researched for breaking through the limitation of the technical barrier.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a reaction solution for nucleic acid purification and recovery, a nucleic acid recovery kit and application thereof, wherein the application is a method for purifying and recovering nucleic acid by using the reaction solution for nucleic acid purification and recovery; the reaction solution for nucleic acid purification and recovery is mixed with a biological sample containing nucleic acid, then alcohol is added, and under the condition of centrifugation, the nucleic acid can be separated from the biological sample. The reaction solution for nucleic acid purification and recovery is matched with alcohols, and the solubility of nucleic acid is changed by the alcohols, so that the nucleic acid is separated from a biological sample and is combined on a nucleic acid solid phase carrier, and the nucleic acid is separated in a single step.
The reaction solution for purifying and recovering nucleic acid comprises salts, a nucleic acid solid phase carrier and water;
the salts are one or more of alkali metal halides, alkaline earth metal halides, halogenated ammonium salt compounds and tris (hydroxymethyl) aminomethane hydrochloride;
the Tris (hydroxymethyl) aminomethane hydrochloride is Tris-HCl with the pH value of 7.5;
the molar concentration of the salt in water is 30-4 mol/L;
the nucleic acid solid phase carrier is polyacrylamide and/or xanthan gum, more preferably polyacrylamide and xanthan gum, wherein the mass concentration of the nucleic acid solid phase carrier in water is 0.5-6 mg/mL.
When the salt is Tris-HCl with the pH value of 7.5, the molar concentration of the salt in water is preferably 30-80 mmol/L;
when the salt is alkaline earth metal chloride, the molar concentration of the salt in water is preferably 50 mmol/L-0.6 mol/L;
when the salt is alkali metal chloride, the molar concentration of the salt in water is preferably 2-4 mol/L.
When the nucleic acid solid phase carrier is polyacrylamide, the mass concentration of the nucleic acid solid phase carrier in water is preferably 3-6 mg/mL;
when the nucleic acid solid phase carrier is xanthan gum, the mass concentration of the nucleic acid solid phase carrier in water is preferably 0.5-3 mg/mL.
The invention relates to a preparation method of a reaction liquid for nucleic acid purification and recovery, which comprises the following steps:
step 1: nucleic acid solid phase carrier pretreatment
Placing the nucleic acid solid phase carrier in a dialysis bag with the molecular weight cutoff of 8000-;
step 2:
according to the proportion, the salt and the purified nucleic acid solid phase carrier are mixed in water to obtain reaction liquid for purifying and recycling nucleic acid.
In the step 1, the purification time is 1-2 days.
The nucleic acid recovery kit of the invention comprises the reaction solution for nucleic acid purification and recovery and a reagent container.
In the nucleic acid recovery kit, the reagent container is used for containing reaction liquid for purifying and recovering nucleic acid.
A nucleic acid separation reagent comprising the reaction solution for nucleic acid purification and recovery and an alcohol;
the alcohol comprises polyethylene glycol 8000 and/or low molecular weight alcohol, wherein the low molecular weight alcohol can be selected from ethanol and/or isopropanol.
The nucleic acid separation reagent is applied to nucleic acid purification and recovery.
The application method of the nucleic acid separation reagent comprises the following steps:
(1) adding a biological sample containing nucleic acid into a nucleic acid recovery kit, uniformly mixing the biological sample containing nucleic acid and reaction liquid for nucleic acid purification and recovery, and adding alcohol to obtain mixed liquid; the pH value of the mixed solution is 6-9;
separating and purifying the mixed solution at the combination temperature of 15-30 ℃ until the precipitate is completely separated out to obtain a precipitate mixture; wherein, according to the volume ratio, the biological sample containing nucleic acid: reaction solution for nucleic acid purification and recovery 1: 1;
(2) separating solid phase substances in the precipitation mixture to obtain a nucleic acid solid phase carrier loaded with nucleic acid;
(3) adding the nucleic acid-loaded nucleic acid solid phase carrier into 70% ethanol aqueous solution for multiple times, cleaning, removing impurities on nucleic acid to obtain an impurity-removed nucleic acid-loaded solid phase carrier, air-drying, adding ultrapure water or double-distilled water into the impurity-removed nucleic acid-loaded solid phase carrier, soaking, and oscillating until the solid is dissolved to obtain the recovered nucleic acid.
In the step (1), preferably, the alcohol includes polyethylene glycol 8000 and/or low molecular weight alcohol, which is intended to change nucleic acid in a biological sample containing nucleic acid from a soluble state to an insoluble state, thereby binding to the nucleic acid solid phase carrier under the action of ions;
when the alcohol is low molecular weight alcohol, and the specific low molecular weight alcohol is ethanol, the volume percentage concentration of the ethanol in the mixed solution is preferably 70-80%;
preferably, when the low molecular weight alcohol is isopropanol, the concentration of the isopropanol in the mixed solution is preferably 35-50% by volume;
preferably, when the alcohol is polyethylene glycol 8000, the mass percentage concentration of the polyethylene glycol 8000 in the mixed solution is preferably 10-30%; wherein the mixed solution is a mixture of reaction solution for purifying and recovering nucleic acid, a biological sample containing nucleic acid and alcohol;
when the alcohol is a mixture of polyethylene glycol 8000 and low-molecular-weight alcohol, the mass percentage concentration of the alcohol in the mixed solution is 10-80%.
In the step (2), the solid-phase substance is separated by centrifugal separation, and the mixed solution is centrifuged for 1-5min under the centrifugal force of 1000-3000 g.
The biological sample containing nucleic acid comprises one or more nucleic acid bands.
The biological sample containing nucleic acid is especially suitable for recovering nucleic acid bands with fragments of more than 100 bp.
The reagent for separating nucleic acid of the present invention has an average recovery rate of 95% or more for nucleic acid bands from which fragments of 100bp or more are recovered and an average recovery rate of 60% or more for nucleic acid bands from which fragments of 50bp to 100bp are recovered.
The reaction solution for nucleic acid purification and recovery, the nucleic acid recovery kit and the application have the advantages that:
1. in the application of the nucleic acid separation reagent, the solubility of nucleic acid and the nucleic acid solid phase carrier in the reaction solution for purifying and recovering the nucleic acid is changed by adding alcohol under the mixing state, so that the nucleic acid is combined on the nucleic acid solid phase carrier, and the reaction solution for purifying and recovering the nucleic acid can be used for purifying enzyme digestion reaction solution and PCR amplification system nucleic acid mixture.
2. The reaction solution for nucleic acid purification and recovery is suitable for purification and recovery of all types of nucleic acids, and has the advantages of simple process, low cost, energy conservation, high efficiency and low flux.
3. The nucleic acid recovery kit can efficiently remove salt ions and free fluorescent substances in a BigDye sequencing reaction mixture. The nucleic acid recovery kit precipitates the DNA product to the bottom of the tube by low-speed centrifugation, and is tracked and labeled by blue substances for convenient observation. The nucleic acid recovery kit has the advantages of simple operation; can completely remove salt ions and free fluorescent substances while efficiently recovering DNA small molecular weight fragments, and can not cause background influence on sequence determination.
Drawings
FIG. 1 is a comparison graph of the purification results of PCR nucleic acid amplification products and the purification results of the same type of products obtained by the purification recovery reaction in example 1 of the present invention;
FIG. 2 is an agarose electrophoresis analysis chart of the recovery results of the purification and recovery reaction of example 2 of the present invention on nucleic acid fragments of different sizes.
Detailed Description
In order to make the aforementioned and other objects, features and advantages of the present invention comprehensible, preferred embodiments accompanied with figures are described in detail below.
The present invention provides a nucleic acid isolation reagent. In the present invention, after the nucleic acid isolation reagent is mixed and reacted with a biological sample, the nucleic acid in the biological sample can be isolated by centrifugation.
In one embodiment, the nucleic acid isolation reagent of the present invention includes polyethylene glycol (PEG)8000, and preferably, the PEG8000 accounts for 10 to 30% by weight of the mixture.
In another embodiment, in the nucleic acid isolation reagent of the present invention, the polyethylene glycol 8000 may be replaced with a low molecular weight alcohol, specifically, absolute ethanol, and preferably, the absolute ethanol accounts for 70 to 80% of the volume concentration of the mixed solution.
The reaction solution for nucleic acid purification and recovery of the present invention comprises salts, which can be any salts suitable for molecular biological experiments, and the salts include but are not limited to one or more of alkali metal halides, alkaline earth metal halides, amine halide salts, Tris-HCl compounds, wherein the cations of the salts are: li+、Na+、K+、Be2+、Mg2+、Ca2+、NH4+One or more of them. The halogen element in the salt of the present invention is preferably Cl-The commonly used salts are LiCl, NaCl, Tris-HCl or the like.
The reaction solution for nucleic acid purification and recovery of the present invention comprises a nucleic acid solid phase carrier, which may be polyacrylamide and/or xanthan gum.
In the biological sample containing nucleic acid according to the present invention, the nucleic acid in the sample can be any natural or synthetic DNA molecule.
The DNA contained in the biological sample containing nucleic acid according to the present invention is dissolved in a solution. The biological sample containing nucleic acid may be a DNA-salt mixture, or a DNA-bio-enzyme-salt mixture, or the like.
After the nucleic acid separation reagent is uniformly mixed with a biological sample containing nucleic acid, the solubility of the nucleic acid can be changed, so that the nucleic acid can be more easily attached to a nucleic acid solid phase carrier, and the purpose of separation can be achieved through high-speed centrifugation.
The process for purifying and recovering nucleic acid by using the nucleic acid separation reagent further comprises the following steps: washing procedure, the nucleic acid is pasted on the solid phase carrier, and after centrifugal precipitation, the nucleic acid can be washed by ethanol with 70% volume concentration, which mainly removes impurities such as salts, proteins, polysaccharides, lipid or cell debris and the like on the nucleic acid.
The reaction solution for nucleic acid purification and recovery and the nucleic acid purification kit based on the reaction solution for nucleic acid purification and recovery can be applied to purification of a PCR system, enzyme digestion and purification of a ligation reaction system.
The present application is described in further detail below by way of specific PCR system purification examples and figures. The following examples are intended to be illustrative of the present application only and should not be construed as limiting the present application.
Example 1
Nucleic acid isolation of PCR nucleic acid amplification systems
The reaction solution used in this example for purification and recovery of nucleic acid contained salts, nucleic acid solid phase carrier and water, wherein the salts were 3mol/L NaCl, the nucleic acid solid phase carrier was 4.5mg/mL polyacrylamide and 0.8mg/mL xanthan gum.
A nucleic acid separation reagent comprises the reaction solution for nucleic acid purification and recovery and also comprises absolute ethyl alcohol.
PCR nucleic acid amplification system
| Amount used (μ L) | |
| Nucleic acid template | 1 |
| PCR primer 1(50 pmol/. mu.L) | 1 |
| PCR primer 2(50 pmol/. mu.L) | 1 |
| PCR mix | 25 |
| dd water | 22 |
| Total volume | 50 |
PCR nucleic acid amplification conditions, 95 ℃ for 5min, (95 ℃ for 10s, 60 ℃ for 15s, 72 ℃ for 15s)30cycles, 72 ℃ for 5min, 4 ℃ hold.
In this embodiment, a nucleic acid isolation reagent for use in a method for purifying and recovering nucleic acid in a PCR nucleic acid amplification system includes the following steps:
1. directly mixing 50 mu L of reaction liquid for nucleic acid purification and recovery with 50 mu L of reaction liquid of a PCR nucleic acid amplification system, uniformly mixing by using a vortex oscillator at room temperature (15-25 ℃), adding 400 mu L of absolute ethyl alcohol, uniformly mixing by using the vortex oscillator until precipitates are completely separated out to obtain a precipitate mixture;
and (3) placing the precipitate mixture in a centrifuge, centrifuging for 5 minutes to remove the aqueous solution, and leaving transparent precipitate at the bottom of the tube to obtain the nucleic acid solid phase carrier loaded with nucleic acid.
2. Washing the transparent precipitate with 1000 μ L70% ethanol solution, centrifuging in a centrifuge for 3 min to remove ethanol solution, collecting transparent precipitate at the bottom of the tube, and drying at room temperature for 5-10 min. This example was precipitated by dissolving the product with 50 μ L of purified water.
3. The experimental group is repeated twice to obtain an experimental group 1 and an experimental group 2, and the concentration and the purity of the recovered product are detected by using a KAIAO 5600 micro spectrophotometer.
4. In the control group of the examples, a commercially available Cycle-Pure Kit from Omega company is used as a positive control group, which represents a conventional column separation and recovery method, and includes the steps of binding, washing, and the like, and the control group repeats two experiments to obtain a control group 1 and a control group 2. The concentration and purity of the recovered product were determined using a KAIAO 5600 microspectrophotometer.
And (3) carrying out electrophoresis on the obtained purified products of the experimental group and the control group by using 2% TAE agarose gel, wherein the loading sequence is 10 microliter of each loading hole in the PCR sample, the control group 1, the control group 2, the experimental group 1 and the experimental group 2. The electrophoresis results are shown in FIG. 1.
| 260/230 | 260/280 | Nucleic acid concentration (ng/. mu.L) | Recovery (%) | |
| PCR sample | 2.083 | 1.859 | 187 | |
| Experimental group 1 | 2.145 | 1.873 | 177 | 94.65 |
| Experimental group 2 | 2.058 | 1.824 | 181 | 96.79 |
| Control group 1 | 2.043 | 1.894 | 178 | 95.19 |
| Control group 2 | 2.127 | 1.841 | 169 | 90.37 |
The analysis of the separated DNA revealed that the average recovery rate of the reaction solution for nucleic acid purification and recovery of the present invention was 95.72% and the average recovery rate of the control group was 92.78%, respectively, indicating that the reaction solution for nucleic acid purification and recovery and the kit of the present invention have very high recovery efficiency for 1500bpDNA fragments.
Example 2
The reaction solution for nucleic acid purification and recovery used in this example contained salts, nucleic acid solid phase carriers and water, wherein the salts were sodium chloride at a molar concentration of 3mol/L, polyacrylamide at a molar concentration of 4.5mg/ml and xanthan gum at a molar concentration of 1.5 mg/ml.
A nucleic acid separation reagent comprises the reaction solution for nucleic acid purification and recovery and also comprises absolute ethyl alcohol.
Markers with the sizes of 50bp, 100bp, 300bp, 400bp, 500bp, 600bp, 800bp, 1000bp, 1500bp and 2000bp are taken as samples and recovered by adopting a nucleic acid separation reagent.
In this embodiment, a nucleic acid isolation reagent for purifying and recovering nucleic acid includes the following steps:
1. taking 50 mu L of reaction liquid for purifying and recycling nucleic acid, directly mixing the reaction liquid with 50 mu L of marker sample, shaking and uniformly mixing the reaction liquid with a vortex oscillator at room temperature (15-25 ℃), adding 400 mu L of absolute ethyl alcohol, shaking and uniformly mixing the reaction liquid with the vortex oscillator until the precipitate is completely separated out to obtain a precipitate mixture;
placing the precipitate mixture in a centrifuge, centrifuging at least 10000g for 5min, removing the water solution, and leaving transparent precipitate at the bottom of the tube.
2. Washing the transparent precipitate with 1000 μ L70% ethanol solution, centrifuging in a centrifuge for 3 min to remove ethanol solution, collecting transparent precipitate at the bottom of the tube, and drying at room temperature for 5-10 min. This example was precipitated by dissolving the product with 50 μ L of purified water.
3. The experiment group is repeated four times to respectively form an experiment group 1, an experiment group 2, an experiment group 3 and an experiment group 4.
4. The recovered DNA solution was purified and subjected to electrophoresis using a 3% TAE agarose gel.
5. The sample loading sequence is marker sample, experiment group 1, experiment group 2, experiment group 3 and experiment group 4, the sample loading amount of each sample loading hole is 10 microliter, and the obtained electrophoresis result is shown in figure 2.
The result shows that the invention can effectively recover the fragments with the length of more than 100bp, and the 50bp fragments also have higher recovery effect, but the recovery rate is only 60 percent. Therefore, the product has great advantages for the construction of the library of the sample type with the free DNA such as partial fragments above 100 bp. However, since a small portion of the fragment smaller than 100bp is removed, the recovery efficiency is low relative to that of the fragment of 100bp or more.
Comparative example 1
A method for separating nucleic acid in a PCR nucleic acid amplification system, which is similar to example 1, except that a reaction solution for purifying and recovering nucleic acid does not include a nucleic acid solid phase carrier;
when the recovered nucleic acid fragment is less than 200bp, the recovery rate of nucleic acid is only 50% at most.
By comparison with example 1, it is demonstrated that the recovery rate of nucleic acid can be significantly improved and the recovered small molecule fragments are smaller by adding a nucleic acid solid phase carrier to the reaction solution for nucleic acid purification and recovery.
Comparative example 2
A nucleic acid isolation method of a PCR nucleic acid amplification system is the same as that in example 1, except that the nucleic acid solid phase carrier in the reaction solution for nucleic acid purification and recovery is 0.8mg/mL xanthan gum;
the recovery rate of nucleic acid is 40%, and the minimum recovered nucleic acid fragment is 50 bp.
By comparison with example 1, it is demonstrated that, if the nucleic acid solid phase carrier is one species, the nucleic acid recovery rate can be significantly improved by adding the nucleic acid solid phase carrier to the reaction solution for purification and recovery of nucleic acid.
Claims (10)
1. The reaction solution for purifying and recovering nucleic acid is characterized by comprising salts, a nucleic acid solid phase carrier and water;
the salts are one or more of alkali metal halides, alkaline earth metal halides, halogenated ammonium salt compounds and tris (hydroxymethyl) aminomethane hydrochloride;
the molar concentration of the salt in water is 30-4 mol/L;
the nucleic acid solid phase carrier is polyacrylamide and/or xanthan gum, wherein the mass concentration of the nucleic acid solid phase carrier in water is 0.5-6 mg/mL.
2. The reaction solution for nucleic acid purification and recovery according to claim 1, wherein when the salt is Tris-HCl with a pH of 7.5, the molar concentration thereof in water is 30 to 80 mmol/L;
when the salt is alkaline earth metal chloride, the molar concentration of the salt in water is 50 mmol/L-0.6 mol/L;
when the salt is alkali metal chloride, the molar concentration of the salt in water is 2-4 mol/L;
when the nucleic acid solid phase carrier is polyacrylamide, the mass concentration of the nucleic acid solid phase carrier in water is 3-6 mg/mL;
when the nucleic acid solid phase carrier is xanthan gum, the mass concentration of the nucleic acid solid phase carrier in water is 0.5-3 mg/mL.
3. The reaction solution for nucleic acid purification and recovery according to claim 1, wherein the nucleic acid solid phase carrier is 4.5mg/mL polyacrylamide and 0.8mg/mL xanthan gum.
4. The method for preparing a reaction solution for nucleic acid purification and recovery according to any one of claims 1 to 3, comprising the steps of:
step 1: nucleic acid solid phase carrier pretreatment
Placing the nucleic acid solid phase carrier in a dialysis bag with the molecular weight cutoff of 8000-;
step 2:
according to the proportion, the salt and the purified nucleic acid solid phase carrier are mixed in water to obtain reaction liquid for purifying and recycling nucleic acid.
5. A kit for nucleic acid isolation comprising the reaction solution for nucleic acid isolation and isolation according to any one of claims 1 to 3 and a reagent container.
6. The kit for nucleic acid isolation according to claim 5, wherein the reagent container is used for containing a reaction solution for nucleic acid purification and isolation.
7. A nucleic acid isolation reagent comprising the reaction solution for nucleic acid purification and recovery according to any one of claims 1 to 3 and an alcohol;
the alcohol comprises polyethylene glycol 8000 and/or low molecular weight alcohol, wherein the low molecular weight alcohol is ethanol and/or isopropanol;
when the alcohol is low molecular weight alcohol, and the specific low molecular weight alcohol is ethanol, the ethanol accounts for 70-80% of the volume percentage concentration of the mixed solution;
when the low molecular weight alcohol is isopropanol, the isopropanol accounts for 35-50% of the volume percentage concentration of the mixed solution;
when the alcohol is polyethylene glycol 8000, the mass percentage concentration of the polyethylene glycol 8000 in the mixed solution is 10-30%; wherein the mixed solution is a mixture of reaction solution for purifying and recovering nucleic acid, a biological sample containing nucleic acid and alcohol;
when the alcohol is a mixture of polyethylene glycol 8000 and low-molecular-weight alcohol, the mass percentage concentration of the alcohol in the mixed solution is 10-80%.
8. The use of the nucleic acid isolation reagent according to claim 7, wherein the method comprises:
(1) adding a biological sample containing nucleic acid into a nucleic acid recovery kit, uniformly mixing the biological sample containing nucleic acid and reaction liquid for nucleic acid purification and recovery, and adding alcohol to obtain mixed liquid; the pH value of the mixed solution is 6-9;
separating and purifying the mixed solution at the combination temperature of 15-30 ℃ until the precipitate is completely separated out to obtain a precipitate mixture; wherein, according to the volume ratio, the biological sample containing nucleic acid: reaction solution for nucleic acid purification and recovery 1: 1;
(2) separating solid phase substances in the precipitation mixture to obtain a nucleic acid solid phase carrier loaded with nucleic acid;
(3) adding the nucleic acid-loaded nucleic acid solid phase carrier into 70% ethanol aqueous solution for multiple times, cleaning, removing impurities on nucleic acid to obtain an impurity-removed nucleic acid-loaded solid phase carrier, air-drying, adding ultrapure water or double-distilled water into the impurity-removed nucleic acid-loaded solid phase carrier, soaking, and oscillating until the solid is dissolved to obtain the recovered nucleic acid.
9. The use of the nucleic acid isolation reagent according to claim 8, wherein the nucleic acid-containing biological sample comprises one or more nucleic acid bands.
10. The use of the nucleic acid isolation reagent according to claim 8, wherein the average recovery rate of the nucleic acid isolation reagent for recovering a fragment of 100bp or more is 95% or more, and the average recovery rate of the nucleic acid isolation reagent for recovering a fragment of 50bp to 100bp is 60% or more.
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| CN115738944A (en) * | 2022-11-15 | 2023-03-07 | 北京擎科生物科技有限公司 | Method and system for recovering nucleic acid synthesis reagent |
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