CN111466609A - Preparation method of momordica grosvenori extract for treating cigarettes by using enzyme preparation - Google Patents

Abstract

The invention discloses a preparation method of an enzyme preparation for treating a Luo Han Guo extract for tobacco use. Enzymatic hydrolysis reaction for 2 to 7 hours; high temperature treatment at 105 to 120 ° C to inactivate the enzyme in the enzyme preparation; 50% to 90% of Luo Han Guo treated with the enzyme preparation according to the ratio of material to liquid 1:4 to 1:10 The ethanol aqueous solution was extracted twice by refluxing at 40-80 °C for 1-6 h each time; the two extracts were combined, filtered with suction after precipitation, and the filtrate was concentrated under reduced pressure to remove the solvent, to obtain a relative density of 1.20-1.36. Luo Han Guo extract for smoking. The Luo Han Guo extract obtained by the enzyme preparation of the present invention is added to cigarettes, which can soften and delicate smoke, increase sweetness, reduce irritation, improve throat comfort, and make up for the application of Luo Han Guo in the prior art in the field of tobacco flavors and fragrances. blank.

Description

Preparation method of extract of Luo Han Guo for tobacco treated with enzyme preparation

technical field

The present invention relates to the technical field of preparing tobacco extract, in particular to a preparation method and application of an enzyme preparation for treating tobacco Luo Han Guo extract.

Background technique

In tobacco products, the aroma and taste of tobacco are very important, and adding flavor is a key link in the cigarette process. Tobacco flavors can be divided into chemically synthesized flavors and natural flavors. In recent years, natural plant flavors have become a hot spot in research and development due to their Chinese characteristics, natural harmlessness, and easy availability of raw materials. The application of biological enzymatic hydrolysis technology to the production and preparation of flavors and fragrances is also an effective means to improve product style and improve the quality of cigarette aroma.

Luo Han Guo, the fruit of the Cucurbitaceae perennial vine. Alias Lahan fruit, fake bitter gourd, light fruit wood turtle, Jinbuhuan, Luohan table, naked tortoise, known as "fairy fruit", its leaves are heart-shaped, dioecious, blooming in summer and fruiting in autumn. Monk fruit is a precious local product in Guilin, and it is also one of the first batch of medicine and food materials approved by the state. Luo Han Guo has high nutritional value and is rich in vitamins and glycosides, fructose, glucose, protein, lipids, etc. Luo Han Guo is sweet and cool in nature. It belongs to the lung and large intestine meridians. It has the effects of moistening the lungs and relieving cough, producing body fluid and quenching thirst.

Adding Luo Han Guo extract to cigarettes has the effect of enriching the smoke and sweetening the smoke. However, the Luo Han Guo extract obtained by the conventional extraction method inevitably contains macromolecular substances such as protein, starch, cellulose, etc. Therefore, adding it to cigarettes has a negative effect of being irritating.

The compound enzyme preparation is applied to extract Luo Han Guo extract, which can hydrolyze starch, protein, cellulose and other macromolecular substances into small molecular substances such as monosaccharides, polypeptides, amino acids, etc., and promote the aroma of small molecules in Luo Han Guo to be released. Synthesis, transformation and accumulation can not only eliminate the negative effects of macromolecular substances on cigarettes, but also play a role in increasing the fragrance and fragrance in cigarettes.

In the process of enzyme treatment, the applicant found that the reaction of biological enzymes has a high degree of specificity, so using a single enzyme preparation to treat Luo Han Guo has a single degradation of the macromolecular components of Luo Han Guo, and its effect is one-sided, improving the performance of Luo Han Guo extract for tobacco use. Quality requires the synergy of multiple enzymes to achieve the best results.

At present, there is no report on the application of the extract prepared by enzymatic hydrolysis of Luo Han Guo in the field of tobacco flavors and fragrances.

SUMMARY OF THE INVENTION

Aiming at the defect that the Luo Han Guo extract for cigarettes obtained by the conventional extraction method has macromolecular substances such as protein, starch and cellulose, and is highly irritating when added to cigarettes, the present invention provides a compound enzyme preparation for the preparation of the Luo Han Guo extract. Method, the method first carries out biological enzymatic hydrolysis of Luo Han Guo, and then prepares the extract. The obtained extract has authentic aroma and good fluidity. It can be added to cut tobacco in a certain proportion, which can soften and delicate smoke, increase sweetness and reduce irritation. Improve throat comfort.

In order to achieve this purpose, the preparation method of a kind of Luo Han Guo extract for tobacco provided by the present invention comprises the following steps:

(1) pulverize Luo Han Guo to 20-60 mesh;

(2) Add a mixed enzyme preparation with 20% to 50% mass of Luo Han Guo, and carry out the enzymatic hydrolysis reaction at 40 to 55°C for 2 to 7 hours;

(3) high temperature treatment at 105～120℃ to inactivate the enzyme in the enzyme preparation;

(4) Add 50%-90% ethanol aqueous solution to the Luo Han Guo treated with the enzyme preparation at a material-to-liquid ratio of 1:4-1:10, and reflux and extract twice at 40-80°C for 1-6 hours each time;

(5) Combine the two extracts, filter them with suction after precipitation, and concentrate the filtrate under reduced pressure to remove the solvent, to obtain the Luo Han Guo extract for cigarettes with a relative density of 1.20-1.36.

The composite enzyme preparation described in the step (2) is composed of: α-amylase, cellulase, laccase, pectinase, flavor protease, enzyme activity enhancer, deionized water; its formula is as shown in Table 1. Show:

Table 1 Formula of compound enzyme preparation

The present invention has the following beneficial effects:

(1) The present invention achieves the synergistic catalytic effect between different enzymes by screening and optimizing the combination of single enzymes, and obtains a composite enzyme preparation that can be used to improve the quality of Luo Han Guo extract for tobacco.

(2) The Luo Han Guo extract for cigarettes obtained by the treatment of the compound enzyme preparation of the present invention has a mellow aroma and good fluidity, and is added to the cut tobacco in a certain proportion, which can soften and delicate smoke, increase the sweetness, reduce irritation, and improve throat comfort. sense.

The present invention firstly undergoes biological enzymatic hydrolysis, and then prepares the extract, which makes up the blank of the prior art in the application of Luo Han Guo in the field of tobacco flavors and fragrances.

Detailed ways

Below in conjunction with specific embodiment, the present invention is described in further detail:

Embodiment 1: the preparation method of Luo Han Guo extract for tobacco of the present invention, comprises the following steps:

(1) pulverize Luo Han Guo to 20 meshes;

(2) Add a compound enzyme preparation with 50% mass of Luo Han Guo (the formula of the enzyme preparation is: α-amylase 0.1%, cellulase 1.0%, laccase 0.1%, pectinase 0.5%, flavor protease 1.5%, enzyme activity Enhancer 20%, deionized water 76.8%), enzymatic hydrolysis reaction at 40°C for 4h;

(3) high temperature treatment at 110°C to inactivate the enzyme in the enzyme preparation;

(4) Add 70% ethanol aqueous solution to the Luo Han Guo treated with the enzyme preparation at a ratio of 1:5, reflux and extract twice at 60°C for 3 hours each time;

(5) The two extracts were combined, filtered with suction after the precipitation, and the filtrate was concentrated under reduced pressure to remove the solvent to obtain a Luo Han Guo extract for tobacco with a relative density of 1.32.

In the present invention, Luo Han Guo is first subjected to biological enzymolysis, and then the extract is prepared, and the obtained Luo Han Guo extract is added to the cigarette, which can soften and delicate smoke, increase sweetness, reduce irritation, improve throat comfort, and make up for the application of Luo Han Guo. It is a gap in the prior art in the field of tobacco flavors and fragrances.

Embodiment 2: the preparation method of Luo Han Guo extract for tobacco of the present invention, comprises the following steps:

(1) pulverize Luo Han Guo to 40 meshes;

(2) Add a compound enzyme preparation with 40% mass of Luo Han Guo (the formula of the enzyme preparation is: α-amylase 1.0%, cellulase 1.5%, laccase 0.2%, pectinase 0.8%, flavor protease 1.5%, enzyme activity Enhancer 20%, deionized water 75%), enzymatic hydrolysis reaction at 45°C for 5h;

(3) high temperature treatment at 115°C to inactivate the enzyme in the enzyme preparation;

(4) Add 60% ethanol aqueous solution to the Luo Han Guo treated with the enzyme preparation at a ratio of 1:6, reflux and extract twice at 50°C, 4 hours each time;

(5) Combine the two extracts, filter with suction after the precipitation, and concentrate the filtrate under reduced pressure to remove the solvent to obtain a Luo Han Guo extract with a relative density of 1.28.

In the present invention, Luo Han Guo is first subjected to biological enzymolysis, and then the extract is prepared, and the obtained Luo Han Guo extract is added to the cigarette, which can soften and delicate smoke, increase sweetness, reduce irritation, improve throat comfort, and make up for the application of Luo Han Guo. It is a gap in the prior art in the field of tobacco flavors and fragrances.

Embodiment 3: the preparation method of Luo Han Guo extract for tobacco of the present invention, comprises the following steps:

(1) pulverize Luo Han Guo to 50 meshes;

(2) Add a compound enzyme preparation with a mass of 35% of Luo Han Guo (the formula of the enzyme preparation is: α-amylase 0.5%, cellulase 2.0%, laccase 0.15%, pectinase 0.8%, flavor protease 2.0%, enzyme activity Enhancer 20%, deionized water 74.55%), enzymatic hydrolysis reaction at 55°C for 4h;

(3) high temperature treatment at 110°C to inactivate the enzyme in the enzyme preparation;

(4) Add 80% ethanol aqueous solution to the Luo Han Guo treated with the enzyme preparation according to the ratio of material to liquid 1:5, and reflux for extraction twice at 50°C for 4 hours each time;

(5) The two extracts were combined, filtered with suction after the precipitation, and the filtrate was concentrated under reduced pressure to remove the solvent to obtain a Luo Han Guo extract for tobacco with a relative density of 1.30.

In the present invention, Luo Han Guo is first subjected to biological enzymolysis, and then the extract is prepared, and the obtained Luo Han Guo extract is added to the cigarette, which can soften and delicate smoke, increase sweetness, reduce irritation, improve throat comfort, and make up for the application of Luo Han Guo. It is a gap in the prior art in the field of tobacco flavors and fragrances.

Embodiment 4: the preparation method of Luo Han Guo extract for tobacco of the present invention, comprises the following steps:

(1) pulverize Luo Han Guo to 60 meshes;

(2) Add a compound enzyme preparation with 40% of Luo Han Guo mass (the formula of the enzyme preparation is: α-amylase 0.8%, cellulase 1.0%, laccase 0.2%, pectinase 0.8%, flavor protease 1.8%, enzyme activity Enhancer 20%, deionized water 75.4%), enzymatic hydrolysis reaction at 50°C for 3h;

(3) high temperature treatment at 110°C to inactivate the enzyme in the enzyme preparation;

(4) Add 80% aqueous ethanol solution to the Luo Han Guo treated with the enzyme preparation at a ratio of 1:5, reflux and extract twice at 60°C for 3 hours each time;

(5) The two extracts were combined, filtered with suction after precipitation, and the filtrate was concentrated under reduced pressure to remove the solvent to obtain a Luo Han Guo extract with a relative density of 1.25.

In the present invention, Luo Han Guo is first subjected to biological enzymolysis, and then the extract is prepared, and the obtained Luo Han Guo extract is added to the cigarette, which can soften and delicate smoke, increase sweetness, reduce irritation, improve throat comfort, and make up for the application of Luo Han Guo. It is a gap in the prior art in the field of tobacco flavors and fragrances.

Embodiment 5: the preparation method of Luo Han Guo extract for tobacco of the present invention, comprises the following steps:

(1) pulverize Luo Han Guo to 30 meshes;

(2) Adding 50% of Luo Han Guo's compound enzyme preparation (the formula of the enzyme preparation is: α-amylase 0.8%, cellulase 1.2%, laccase 0.2%, pectinase 0.8%, flavor protease 2.0%, enzyme activity Enhancer 20%, deionized water 75%), enzymolysis reaction at 40°C for 6h;

(3) high temperature treatment at 110°C to inactivate the enzyme in the enzyme preparation;

(4) Add 60% aqueous ethanol solution to the Luo Han Guo treated with the enzyme preparation at a ratio of 1:6, reflux and extract twice at 60°C for 5 hours each time;

(5) The two extracts were combined, filtered with suction after precipitation, and the filtrate was concentrated under reduced pressure to remove the solvent to obtain a Luo Han Guo extract with a relative density of 1.25.

In the present invention, Luo Han Guo is first subjected to biological enzymolysis, and then the extract is prepared, and the obtained Luo Han Guo extract is added to the cigarette, which can soften and delicate smoke, increase sweetness, reduce irritation, improve throat comfort, and make up for the application of Luo Han Guo. It is a gap in the prior art in the field of tobacco flavors and fragrances.

The above-mentioned embodiments are only intended to illustrate the technical concept and characteristics of the present invention, and the purpose thereof is to enable those who are familiar with the art to understand the content of the present invention and implement them accordingly, and cannot limit the protection scope of the present invention. All equivalent changes or modifications made according to the spirit of the present invention should be included within the protection scope of the present invention.

Claims (3)

1. A preparation method of a cigarette siraitia grosvenorii extract by enzyme preparation treatment is characterized by comprising the following steps:

(1) crushing the momordica grosvenori to 20-60 meshes;

(2) adding a complex enzyme preparation with the mass ratio of 20-50% of the momordica grosvenori, and carrying out enzymolysis reaction for 2-7 h at the temperature of 40-55 ℃;

(3) carrying out high-temperature treatment at 105-120 ℃ to inactivate enzymes in the enzyme preparation;

(4) adding 50-90% ethanol aqueous solution into the enzyme preparation-treated fructus momordicae according to the material-to-liquid ratio of 1: 4-1: 10, and performing reflux extraction twice at 40-80 ℃ for 1-6 hours each time;

(5) and combining the two extracting solutions, precipitating, filtering, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby preparing the cigarette fructus momordicae extract with the relative density of 1.20-1.36.

2. The preparation method of claim 1, wherein the complex enzyme preparation in step (2) comprises α -amylase, cellulase, laccase, pectinase, flavourzyme, enzyme activity enhancer and deionized water, and the formula is shown in Table 1:

TABLE 1 formulation of Complex enzyme preparation

3. The method of claim 1, comprising the steps of:

(1) crushing the momordica grosvenori to 50 meshes;

(2) adding a complex enzyme preparation accounting for 35 percent of the mass of the momordica grosvenori into the mixture for enzymolysis reaction for 4 hours at the temperature of 55 ℃, wherein the enzyme preparation comprises α -amylase 0.5 percent, cellulase 2.0 percent, laccase 0.15 percent, pectinase 0.8 percent, flavourzyme 2.0 percent, enzyme activity enhancer 20 percent and deionized water 74.55 percent;

(3) treating at 110 deg.C to inactivate enzyme in the enzyme preparation;

(4) adding the momordica grosvenori treated by the enzyme preparation into 80% ethanol water solution according to the material-liquid ratio of 1:5, and performing reflux extraction twice at 50 ℃ for 4 hours each time;

(5) and combining the two extracting solutions, precipitating, carrying out suction filtration, and carrying out reduced pressure concentration on the filtrate to remove the solvent, thereby obtaining the cigarette momordica grosvenori extract with the relative density of 1.30.