CN111437248A - A kind of Yichangshou oral liquid and its preparation method and application - Google Patents

Abstract

The application discloses intestine-benefiting life oral liquid and a preparation method and application thereof, and the prepared intestine-benefiting life oral liquid is suitable for middle-aged and elderly people in a sub-health state. The intestine-benefiting life-prolonging oral liquid has double functions, and on one hand, the intestinal-benefiting life-prolonging oral liquid can promote discharge of heavy metals such as cadmium, lead and the like accumulated in an organism and reduce the toxicity of the heavy metals to the organism; on the other hand, the lactobacillus and bifidobacterium which are intestinal probiotics have obvious proliferation effect, and are beneficial to middle-aged and elderly people in sub-health state to enhance the immunity of the organism and improve the physiological condition of the organism by proliferating the intestinal probiotics. The intestine-benefiting life-prolonging oral liquid is low in cost, free of toxic and side effects and capable of being widely popularized and used.

Description

A kind of Yichangshou oral liquid and its preparation method and application

technical field

The application relates to the technical field of excretion promotion of heavy metals, in particular to a Yichangshou oral liquid and a preparation method and application thereof.

Background technique

With the development of my country's industry, the industrial "three wastes" are increasingly polluting the environment, especially heavy metal pollution, which will cause great harm to the entire biological chain and ecosystem. Some toxic and harmful substances such as cadmium, lead and other heavy metals can migrate to water and soil, enter the human body through the food chain, and bring cumulative harm to human health; in addition, heavy metals can also pass through haze and particulate matter (PM2.5) in dust. The respiratory tract enters the human body.

Although my country requires that the industrial "three wastes" must be purified and discharged up to the standard, the pollution of the "three wastes" is still severe. According to statistics, the world releases about 30,000 tons of cadmium into the environment every year, of which 82% to 94% of the cadmium will enter the soil. It can accumulate in the internal organs of human or livestock through bioconcentration, thereby causing serious damage to organs such as kidneys and bones. For example, in the 1860s in Japan, the "Bone-Itai Disease", that is, cadmium (cadmium, Cd) entered the human body through the food chain. Cadmium is highly toxic, and the air and food polluted by cadmium are seriously harmful to the human body. Slow metabolism, that is, excessive accumulation of cadmium in the body damages the renal tubular function, causing the loss of protein in the body from the urine, and the accumulation of cadmium increases with age. In addition, cadmium can also replace calcium in the body, resulting in osteomalacia and spontaneous fractures over time .

For lead (Plumbum, Pb) poisoning, it is not only more common in children, but also seriously harms adults, causing diseases such as nervous system, digestive system, blood system, cardiovascular system, kidney function damage, reproductive system damage and other diseases , has attracted widespread attention of the society.

At present, less attention is paid to the content of heavy metals in the human body. Generally, the relevant treatment work is only carried out when the human body has symptoms of heavy metal poisoning. At this time, the content of heavy metals in the human body has seriously exceeded the standard and caused damage to the human body. And more damage is irreversible. Therefore, in order to reduce the damage caused by heavy metals to the human body, it is necessary to prevent the long-term accumulation of heavy metals in the human body, especially the middle-aged and elderly people in sub-health state, regularly discharge the heavy metals that enter the body, and control the content of heavy metals in the human body within the standard range In order to avoid the problem of heavy metal poisoning in the human body.

However, there is currently no heavy metal excretion-promoting product on the market that is suitable for the middle-aged and elderly people in sub-health state and can promote excretion of various heavy metals. Therefore, there is an urgent need for a heavy metal detoxification agent that is suitable for middle-aged and elderly people in sub-health state and can simultaneously promote the excretion of various heavy metals.

SUMMARY OF THE INVENTION

The application provides a Yichangshou oral liquid and its preparation method and application, so as to solve the problem that there is no heavy metal excretion promoting effect on the market that is suitable for middle-aged and elderly people in a sub-health state and can promote the excretion of various heavy metals. product problem.

In a first aspect, the application provides a Yichangshou oral liquid, comprising active ingredients, auxiliary ingredients and a solvent, wherein the active ingredients include carboxymethyl chitosan, β-cyclodextrin, fructooligosaccharides and stachyose, The auxiliary ingredients include white peony root, citrus aurantium, raw land, cassia, tuckahoe and radish, and the solvent is water.

Optionally, the mass ratio of carboxymethyl chitosan, β-cyclodextrin, oligofructose and stachyose is 1:1:2:1; Paeonia lactiflora, Citrus aurantium, Helicobacter, Cassia, Poria and The mass ratio of raisins is 1:1:1.5:1.5:1.5:2.

Optionally, the active ingredients include the following raw materials by weight: carboxymethyl chitosan 5g, β-cyclodextrin 5g, fructooligosaccharide 10g, stachyose 5g; the adjuvant components include the following raw materials by weight: Paeonia lactiflora 5g , Citrus aurantium 5g, raw land 7.5g, Cassia 7.5g, Poria 7.5g, Radix radish 10g.

Optionally, the amount of solvent is 200-300 ml.

In a second aspect, the present application also provides a method for preparing the above-mentioned Yichangshou oral liquid, including pulverizing and mixing auxiliary materials, preparing medicinal liquid, introducing active ingredients, viscosity control and sterilization processes.

Wherein, the pulverizing and mixing process of the auxiliary materials specifically includes: pulverizing the Paeonia lactiflora, Citrus aurantium, raw land, Cassia, Poria and Radix Radix in the auxiliary material components, and putting them into a mixer for uniform mixing to obtain mixed auxiliary materials;

The process of preparing the medicinal liquid specifically includes: adding an appropriate amount of water to the mixed auxiliary materials for decocting, maintaining the decocting temperature between 80°C and 95°C, and decocting for about 1 hour, removing the medicinal residues by filtration, and obtaining the upper medicinal liquid. ;

The process of introducing the active ingredients specifically includes: adding carboxymethyl chitosan, β-cyclodextrin, fructooligosaccharides and stachyose to the obtained medicinal liquid, and fully stirring until the active ingredients are dissolved in the medicinal liquid to prepare an oral stock solution;

The viscosity control process specifically includes: adjusting the viscosity of the oral stock solution to prepare the oral solution;

The sterilization process specifically includes: sterilizing the oral liquid, wherein the sterilization time is 10-30 minutes, the sterilization temperature is 110-130° C., and the sterilization pressure is 1.0-1.2kpa.

In a third aspect, the application also provides an application of the above-mentioned Yichangshou oral liquid, which is used as a beneficial additive in dairy products or health care products for the middle-aged and elderly.

The application provides a Yichangshou oral liquid and a preparation method and application thereof, and the prepared Yichangshou oral liquid is suitable for middle-aged and elderly people in sub-health state. The Yichangshou oral liquid of the present application has dual functions. On the one hand, it can adsorb and promote the excretion of heavy metals such as cadmium and lead, and reduce the toxicity of heavy metals to the body; The bacteria Lactobacillus and Bifidobacterium have significant proliferation effect, which helps the middle-aged and elderly people in sub-health state to enhance the immunity of the body and improve the physiological condition of the body by proliferating intestinal probiotics. In addition, the Yichangshou oral liquid of the present application has low cost, no toxic and side effects, and can be widely used.

Description of drawings

In order to illustrate the technical solutions of the present application more clearly, the accompanying drawings required in the embodiments will be briefly introduced below. Obviously, for those of ordinary skill in the art, without creative work, the Additional drawings can be obtained from these drawings.

Fig. 1 is the flow chart of the preparation method of Yichangshou oral liquid of the application,

Figure 2 is the Pb standard curve;

Figure 3 is a Cd standard curve;

Fig. 4 is a graph of changes in body weight of each group of mice within four weeks in the lead poisoning test;

Figure 5 is a graph of changes in the intestinal flora of mice in each group within four weeks in the lead poisoning test;

Fig. 6 is a graph showing the change of lead content in the kidneys of each group of mice within four weeks in the lead poisoning test;

Fig. 7 is a graph of changes in body weight of mice in each group within four weeks in the cadmium poisoning test;

Figure 8 is a graph of changes in the intestinal flora of mice in each group within four weeks in the cadmium poisoning test;

Figure 9 is a graph showing the changes of cadmium content in the kidneys of mice in each group within four weeks in the cadmium poisoning test.

Detailed ways

The application provides a Yichangshou oral liquid, which is suitable for middle-aged and elderly people in a sub-health state, and can adsorb and promote the excretion of heavy metals such as cadmium and lead, and reduce the toxicity of heavy metals to the body.

The Yichangshou oral liquid provided in this application includes active ingredients, auxiliary ingredients and solvents, wherein the active ingredients include carboxymethyl chitosan, β-cyclodextrin, fructooligosaccharides and stachyose, and the auxiliary ingredients include white peony root, citrus aurantium Shell, habitat, Cassia, Poria and Radix, the solvent is water.

Among them, among the active ingredients, the main functions of carboxymethyl chitosan and β-cyclodextrin are to promote the excretion of heavy metals in the body, enhance the immunity of the body, and promote the proliferation of probiotic Lactobacillus in the intestine. The normal dominant bacteria in the tract are beneficial to the host's digestion and absorption, can inhibit pathogenic bacteria, increase the number of lymphocytes in the intestinal tract, and enhance the body's immunity. The main effect of fructooligosaccharides and stachyose is to promote the proliferation of intestinal probiotics bifidobacteria and improve the immunity of the body.

Among the auxiliary components, "white peony root" has the effect of regulating liver and spleen disharmony, headache and dizziness; "citrus aurantium" has a good effect on middle-aged and elderly people's food accumulation, abdominal distention and pain; Defecation, liver protection, anti-tumor, improve immunity; "Cassiae" main effect is to improve eyesight, gallbladder and liver protection, laxative; Heart; "Radix radish" has a good effect on people with food accumulation and stagnation of qi, cough and phlegm, chest tightness and less food.

In this example, the mass ratio of carboxymethyl chitosan, β-cyclodextrin, oligofructose and stachyose is 1:1:2:1; Paeonia lactiflora, Citrus aurantium, Helicobacter, Cassia, Poria and The mass ratio of raisins is 1:1:1.5:1.5:1.5:2.

In this example, the active ingredients include the following raw materials by weight: 5 g of carboxymethyl chitosan, 5 g of β-cyclodextrin, 10 g of fructooligosaccharides, and 5 g of stachyose; the auxiliary ingredients include the following raw materials by weight: 5 g of peony root , Citrus aurantium 5g, raw land 7.5g, Cassia 7.5g, Poria 7.5g, Radix radish 10g.

In this example, the amount of solvent is 200-300ml, and those skilled in the art can adjust the amount of solvent according to actual needs, for example, 250ml, which belongs to the protection scope of the present application.

The present application also provides a method for preparing the above-mentioned Yichangshou oral liquid. FIG. 1 is a flowchart of the preparation method of the Yichangshou oral liquid of the application. As shown in FIG. 1 , the preparation method of the Yichangshou oral liquid includes crushing and mixing of auxiliary materials. , Preparation of liquid medicine, introduction of active ingredients, viscosity control and sterilization process.

Wherein, in step 100, the pulverizing and mixing process of the auxiliary materials specifically includes: pulverizing the Paeonia suffruticosa, Citrus aurantium, raw land, Cassia, Poria and Radix Radix in the auxiliary material components, and putting them into a mixer for uniform mixing to obtain mixed auxiliary materials;

Step 200, the process of preparing the medicinal liquid specifically includes: adding an appropriate amount of water to the mixed auxiliary materials for decoction, the decoction temperature is kept between 80 ° C and 95 ° C, the decoction time is about 1 hour, the medicinal residue is removed by filtration, and the upper layer is obtained. the medicinal solution;

Step 300, the process of introducing the active ingredients specifically includes: adding carboxymethyl chitosan, β-cyclodextrin, fructooligosaccharides and stachyose to the obtained medicinal solution, and fully stirring until the active ingredients are dissolved in the medicinal solution to prepare the solution. Oral solution;

In step 400, the viscosity control process specifically includes: adjusting the viscosity of the oral liquid to prepare the oral liquid. It should be noted that carboxymethyl chitosan and β-cyclodextrin have film-forming properties. After adding carboxymethyl chitosan and β-cyclodextrin to the medicinal solution, in order to prevent the viscosity from being too large, the oral stock solution needs to be adjusted. viscosity. Those skilled in the art can set the viscosity of the oral stock solution according to actual needs, for example, 120 mPa.s, 100 mPa.s or 80 mPa.s, which belongs to the protection scope of the present application. At present, there are various methods for adjusting the viscosity of the oral liquid. For example, water dilution is the most commonly used method. Of course, edible diluents can be added, which all belong to the protection scope of the present application.

Step 500, the sterilization process specifically includes: sterilizing the oral liquid, wherein the sterilization time is 10-30 minutes, the sterilization temperature is 110-130°C, and the sterilization pressure is 1.0-1.2kpa; Those skilled in the art can select suitable sterilization conditions according to actual needs, for example, the sterilization time is 20 minutes, the sterilization temperature is 120°C, and the sterilization pressure is 1.1 kpa, all of which belong to the protection scope of the present application.

In this example, the preparation method of the Yichangshou oral liquid of the present application further includes putting the dried Yichangshou oral liquid into a plastic packaging bottle that meets the hygienic food safety standard for packaging.

The application also provides an application of the above-mentioned Yichangshou oral liquid, which is used as a beneficial additive component in dairy products or health care products for middle-aged and elderly people. As a beneficial ingredient, Yichangshou Oral Liquid can be added to dairy products or health care products for middle-aged and elderly people.

In order to verify the effect of the Yichangshou oral liquid of the present application on promoting the excretion of heavy metals such as cadmium and lead in the body, the following experiments will be carried out with mice as experimental materials.

1. Establishment of a mouse model of heavy metal exposure

1.1 Gavage Yichangshou Oral Liquid

(1) Grouping

112 mice were randomly divided into 7 groups: one blank group, three poisoning groups and three experimental groups, with 16 mice in each group. The blank group was gavaged with normal saline for 2 weeks, and the poisoning group and the experimental group were gavaged with Pb and Cd solutions for 2 weeks, respectively. After each week, 4 rats in each of the three groups were dissected, and the toxic effects of the three heavy metals were observed by comparison among the three groups.

(2) Weighing and feeding

Mice were weighed every morning starting at 9:00 am, and the body weight of each mouse was recorded. After the weighing, the mice in each group were fed with rat food (2g/mice in the daytime and 3g/mice in the evening) according to the weight and quantity of each group of mice, and ensured that the mice had enough cold boiled water.

(3) gavage

From 10:00 in the morning, the mice were given intragastrically according to the body weight of the day. The blank group was given normal saline, the lead poisoning group and the test group were given 10 mg/L lead acetate, and the cadmium poisoning group and the test group were given 0.1 mg/L cadmium solution by gavage, and injected at a volume of 1 ml/10 g.

1.2 Culture of intestinal flora

(1) Collection of feces

Before the mouse intraperitoneal injection, the feces of each mouse were collected by abdominal massage, placed in a sterilized centrifuge tube, and marked. The frequency of collection is once every 2 days.

(2) Excrement treatment

Weigh 0.1 g of the collected feces and dissolve it in 9.9 ml of water to dilute 100 times, and immediately transfer it to the ultra-clean workbench for gradient dilution. The dilution gradient is 106.

(3) Bacteria culture

Pour the four kinds of sterilized culture medium into four kinds of plates on the ultra-clean workbench, turn on the ultraviolet light for 15 minutes, and quickly spread the inoculation after cooling the plates, make a mark, and let it stand for 10 minutes. The two kinds of anaerobic bacteria culture dishes (Lactobacillus, Bifidobacterium) were sealed with sealing strips, put into a constant temperature incubator together with all the culture dishes, and cultivated at 37 °C for 48 hours.

(4) bacterial count

After 48 hours, all petri dishes were taken out, and the four bacterial groups Lactobacillus, Bifidobacterium, Escherichia coli, and Enterococcus were counted and recorded.

1.3 Determination of Pb and Cd content

(1) Dissect mice

Mice selected for dissection were separated individually, and the mice were sacrificed by cervical dislocation, soaked in 75% alcohol for 5 minutes, and transferred to an ultra-clean workbench for dissection. The removed kidneys were placed in sterile packaging bags and stored in a -20°C refrigerator.

(2) Digestion organs

Take out the mouse kidney organ, accurately weigh it, put it in a 50ml polytetrafluoroethylene crucible, add 10ml of nitric acid and cover it and soak it overnight, place the crucible on an electric hot plate to digest at 200 °C, and intermittently add hydrogen peroxide dropwise until it produces Yellow-brown smoke turned to white smoke. The final solution is clear and colorless or slightly yellow, add about 10ml of water and continue to heat to drive off the acid, heat to near dryness, cool, add distilled water and transfer to a 50ml volumetric flask, dilute to the mark with distilled water, and set the volume.

(3) Establishment of standard curve

1) Preparation of Pb standard solution

Measure 10 mL of 0.1 mg/mL Pb single-element stock solution, use a 100 mL volumetric flask to dilute to volume, and shake well to obtain a standard stock solution of Pb with a concentration of 10 μg/mL. Take five dry and clean 50 mL volumetric flasks, and transfer 1 mL, 2 mL, 3 mL, 4 mL, 5 mL, and 6 mL of standard stock solution of 10 μg/mL Pb into them with a pipette, and then determine Volume to the mark, shake well, the standard serial concentrations of Pb in the five volumetric flasks are 0.2 μg/mL, 0.4 μg/mL, 0.6 μg/mL, 0.8 μg/mL, 1.0 μg/mL, 1.2 μg/mL, respectively. Label the concentration respectively, and obtain the standard curve of Pb as shown in Figure 2.

2) Preparation of Cd standard solution

Measure 2 mL of 0.1 mg/mL Cd single-element stock solution, use a 100 mL volumetric flask to dilute to volume, and shake well to obtain a standard stock solution of Cd with a concentration of 2 μg/mL. Take five dry and clean 50 mL volumetric flasks, and use a pipette to transfer 0.25 mL, 1.25 mL, 2.5 mL, 5 mL, 7.5 mL, and 10 mL of standard stock solution of 2 μg/mL Cd into them respectively, and then adjust the volumes respectively. to the mark, shake well, the standard serial concentrations of Cd in the five volumetric flasks are 0.01 μg/mL, 0.05 μg/mL, 0.1 μg/mL, 0.2 μg/mL, 0.3 μg/mL, 0.4 μg/mL, respectively. Label the concentration and get the Cd standard curve as shown in Figure 3.

4) Data processing

According to the standard curve of each heavy metal concentration, the content of heavy metals in the sample solution was calculated. Analysis of heavy metal enrichment in kidneys.

2. Gavage the mice with Yichangshou Oral Liquid

2.1 Gavage Yichangshou Oral Liquid

(1) Grouping

After the first two weeks of the experiment, there were only 8 mice left in each group, for a total of 72 mice in the three groups. Next, the blank group was given normal saline for 2 weeks, the poisoned group was given normal saline for 2 weeks, and the experimental group was given Yichangshou Oral Liquid for 2 weeks. After each week, four animals in each of the three groups were dissected. To observe the detoxification effect of Yichangshou Oral Liquid.

(2) Weighing and feeding

Mice were weighed every morning starting at 9:00 am, and the body weight of each mouse was recorded. After the weighing, the mice in each group were fed with rat food (2g/mice in the daytime and 3g/mice in the evening) according to the weight and quantity of each group of mice, and ensured that the mice had enough cold boiled water.

(3) Mice gavage

From 10:00 in the morning, the mice were given intragastrically according to the body weight of the day. The blank group was intragastrically administered with normal saline, the poisoned group was intragastrically administered with normal saline, and the experimental group was intragastrically administered an aqueous solution of Yichangshou Oral Liquid at a dose of 0.1ml/10g.

2.2 Cultivation of intestinal flora

(1) Collection of feces

Before the mouse intraperitoneal injection, the feces of each mouse were collected by abdominal massage, placed in a sterilized centrifuge tube, and marked. The frequency of collection is once every 2 days.

(2) Excrement treatment

Weigh 0.1 g of the collected feces and dissolve it in 9.9 ml of water to dilute 100 times, and immediately transfer it to the ultra-clean workbench for gradient dilution. The dilution gradient is 106.

(3) Bacteria culture

Pour the four kinds of sterilized culture medium into four kinds of plates on the ultra-clean workbench, turn on the ultraviolet light for 15 minutes, and quickly spread the inoculation after cooling the plates, make a mark, and let it stand for 10 minutes. The two kinds of anaerobic bacteria culture dishes (Lactobacillus, Bifidobacterium) were sealed with sealing strips, and put into a constant temperature incubator together with all the culture dishes, and cultivated at 37OC for 48 hours.

(4) bacterial count

After 48 hours, all petri dishes were taken out, and the four bacterial groups Lactobacillus, Bifidobacterium, Escherichia coli, and Enterococcus were counted and recorded.

2.3 Determination of Pb and Cd content

(1) Dissect mice

Mice selected for dissection were separated individually, and the mice were sacrificed by cervical dislocation, soaked in 75% alcohol for 5 minutes, and transferred to an ultra-clean workbench for dissection. The removed kidneys were placed in sterile packaging bags and stored in a -20°C refrigerator.

(2) Digestion organs

Take out the mouse kidney organ, accurately weigh it, put it in a 50ml polytetrafluoroethylene crucible, add 10ml of nitric acid and cover it and soak it overnight, place the crucible on an electric hot plate to digest at 200 °C, and intermittently add hydrogen peroxide dropwise until it produces Yellow-brown smoke turned to white smoke. The final solution is clear and colorless or slightly yellow, add about 10ml of water and continue to heat to drive off the acid, heat to near dryness, cool, add distilled water and transfer to a 50ml volumetric flask, dilute to the mark with distilled water, and set the volume.

(3) Data processing

The absorbance of the three heavy metal samples was measured, and the content of heavy metals in the sample solution was calculated according to the standard curve of each heavy metal concentration. Analysis of heavy metal enrichment in kidneys.

3. Research results

3.1 Lead poisoning in mice

(1) Weight change

As shown in Figure 4, the body weight of the mice in the blank group increased linearly, while the weight of the mice was decreased within the first week of lead poisoning by gavage with lead acetate. Within four weeks, the overall body weight of the mice increased. Compared with the poisoning group, the body weight recovered after gavage with Yichangshou Oral Liquid.

(2) Changes in intestinal flora

As shown in Figure 5, after gavage of Yichangshou Oral Liquid was started on the fourteenth day, the intestinal probiotics Lactobacillus and Bifidobacterium in the intestinal tract increased significantly.

(3) Lead content in kidney organs

As shown in Figure 6, after lead poisoning, lead was mainly concentrated in the kidneys, and after gavage with Yichangshou Oral Liquid, the lead content in the kidneys decreased.

3.2 Cadmium poisoning in mice

(1) Weight change

As shown in Figure 7, the body weight of the mice in the blank group increased linearly, while the body weight of the mice fed with cadmium solution decreased significantly in the first week. The overall body weight of the mice increased within four weeks. Compared with the poisoning group, the oral administration of Yichangshou Oral Liquid had a promoting effect on the weight recovery of the mice in the experimental group.

(2) Changes in the number of intestines

As shown in Figure 8, after gavage of Yichangshou Oral Liquid was started on the fourteenth day, the intestinal probiotics Lactobacillus and Bifidobacterium in the intestinal tract increased significantly.

(3) Changes in kidney cadmium content

As shown in Figure 9, after cadmium poisoning, lead was distributed in the kidneys, and after gavage with Yichangshou Oral Liquid, the cadmium content in the kidneys decreased.

In summary, judging from the changes in the body weight and appearance characteristics (fur, etc.) of the mice, Pb and Cd exposure had a harmful effect on the mice, and the mice showed obvious loss of appetite, lethargy, and some individuals died. ; The content of Pb and Cd in the kidney was measured, and it was found that there was a significant increase, but there was no significant change in the intestinal flora. After gavage with Yichangshou Oral Liquid, it was found that in terms of body weight and behavior, there were signs of recovery, and the contents of Pb and Cd in the kidneys decreased. The number of fidobacteria also increased.

4 Conclusion

According to the above test results, taking the Yichangshou oral liquid has a certain positive effect on the excretion and detoxification of heavy metal poisoning, and has the effect of promoting the proliferation of intestinal probiotics Lactobacillus and Bifidobacterium in the intestinal tract. Specific effects include:

(1) Yichangshou oral liquid has strong heavy metal adsorption capacity on the one hand, and compared with other heavy metal excretion promoters, the Yichangshou oral liquid of the present application has a good effect in promoting the excretion of various heavy metals in the body, At the same time, because of its good biocompatibility, the toxicity and side effects are small.

(2) On the other hand, the Yichangshou oral liquid of the present application can improve the composition of intestinal flora and promote the proliferation of intestinal probiotics Lactobacillus and Bifidobacterium.

Among them, Lactobacillus reuteri is an important type of intestinal probiotics. For example, Lactobacillus reuteri, which is ubiquitous in vertebrates, has the following beneficial effects. First, Lactobacillus reuteri can produce antibacterial molecules that can Inhibits the colonization of pathogenic microorganisms and remodels the commensal microbiota; second, L. reuteri may benefit the host immune system; Microbial translocation to tissues. Therefore, direct supplementation or prebiotic modulation of Lactobacillus reuteri may be an effective way to prevent and treat inflammatory diseases.

As an important intestinal probiotic, Bifidobacterium has a variety of probiotic functions, including improving intestinal diseases caused by immune system disorders, such as inflammatory bowel disease, ulcerative colitis, Crohn's disease, pouchitis It adheres to intestinal epithelial cells and secretes antimicrobial peptides, while increasing the thickness of the mucus layer and inhibiting the invasion of pathogenic bacteria into the intestine; scavenging free radicals, increasing the activity of host antioxidant enzymes, reducing the content of serum and liver malondialdehyde, thereby relieving the body oxidative damage and delay aging.

According to research, the bifidobacteria in the intestinal tract of infants account for 99% of the total, while the intestinal bifidobacteria of healthy people (26-28 years old) is only 14.8%, and that of the elderly is only 3.2%. Probiotics, such as Bifidobacterium and Lactobacillus, are a class of active microorganisms that are beneficial to the host by colonizing the human body and changing the composition of a certain part of the host's flora. Group balance, promote food digestion and absorption, prevent constipation, synthesize vitamins, thereby enhancing human immunity and improving the body's antioxidant level.

The Yichangshou oral liquid of the present application can improve the composition of intestinal flora, and promote the proliferation of intestinal probiotics Lactobacillus and Bifidobacterium. The effect of heavy metals reduces the toxicity of heavy metals and further reduces the damage of heavy metals to the human body.

The application provides a Yichangshou oral liquid and a preparation method and application thereof, and the prepared Yichangshou oral liquid is suitable for middle-aged and elderly people in sub-health state. The Yichangshou oral liquid of the present application has dual functions. On the one hand, it can adsorb and promote the excretion of heavy metals such as cadmium and lead, and reduce the toxicity of heavy metals to the body; The bacteria Lactobacillus and Bifidobacterium have significant proliferation effects. Intestinal probiotics Lactobacillus and Bifidobacterium can promote intestinal peristalsis, laxative and moisturizing the intestines, relieve the constipation problem of middle-aged and elderly people, inhibit the formation of putrefactive substances in the intestine, and benefit the The gallbladder protects the liver, strengthens the spleen and calms the heart, so as to achieve the effects of delaying aging, improving immunity and improving the physiological condition of the body. In addition, the Yichangshou oral liquid of the present application has low cost, no toxic and side effects, and can be widely used.

The above-described embodiments of the present application do not limit the protection scope of the present application.

Claims (6)

1. An intestine-benefiting and life-prolonging oral liquid is characterized by comprising effective components, auxiliary material components and a solvent, wherein the effective components comprise carboxymethyl chitosan, β -cyclodextrin, fructo-oligosaccharide and stachyose, the auxiliary material components comprise white paeony root, bitter orange, radix rehmanniae, cassia occidentalis, poria cocos and radish seed, and the solvent is water.

2. The intestine-benefiting and life-prolonging oral liquid according to claim 1, wherein the mass ratio of the carboxymethyl chitosan, the β -cyclodextrin, the fructo-oligosaccharide and the stachyose is 1:1:2:1, and the mass ratio of the white paeony root, the bitter orange, the radix rehmanniae, the cassia occidentalis, the poria cocos and the radish seed is 1:1: 1.5: 1.5: 1.5: 2.

3. The intestine-benefiting and life-prolonging oral liquid as claimed in claim 2, wherein the effective components comprise, by weight, 5g of carboxymethyl chitosan, 5g of β -cyclodextrin, 10g of fructo-oligosaccharide and 5g of stachyose, and the auxiliary materials comprise, by weight, 5g of radix paeoniae alba, 5g of fructus aurantii, 7.5g of radix rehmanniae, 7.5g of semen cassiae torae, 7.5g of poria cocos and 10g of semen raphani.

4. The oral liquid for benefiting intestine and longevity according to claim 3, wherein the amount of the solvent is 200-300 ml.

5. A method for preparing the oral liquid for benefiting intestine and prolonging life as claimed in any one of the above claims 1-4, which comprises the steps of crushing and mixing auxiliary materials, preparing liquid medicine, introducing effective components, controlling viscosity and sterilizing,

wherein, the auxiliary material is smashed and is specifically included with the mixing process: pulverizing radix Paeoniae alba, fructus Aurantii, radix rehmanniae, semen Cassiae, Poria and Raphani semen, and mixing in a mixer to obtain mixed adjuvants;

the liquid medicine preparation process specifically comprises the following steps: adding appropriate amount of water into the mixed adjuvants, decocting at 80-95 deg.C for about 1 hr, filtering to remove residue, and collecting the upper layer medicinal liquid;

the step of introducing effective components comprises adding carboxymethyl chitosan, β -cyclodextrin, fructo-oligosaccharide and stachyose into the obtained medicinal liquid, stirring thoroughly until the effective components are dissolved in the medicinal liquid, and making into oral stock solution;

the viscosity control process specifically comprises the following steps: adjusting the viscosity of the oral stock solution to obtain oral liquid;

the sterilization process specifically comprises the following steps: sterilizing the oral liquid, wherein the sterilization time is 10-30 minutes, the sterilization temperature is 110-130 ℃, and the sterilization pressure is 1.0-1.2 kpa.

6. Use of an oral liquid according to any of claims 1 to 4, wherein the oral liquid is used as a beneficial additive in dairy products or health products for the middle-aged and elderly.

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