CN111397998A - Staining solution for tissue cell section - Google Patents
Staining solution for tissue cell section Download PDFInfo
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- CN111397998A CN111397998A CN202010337519.4A CN202010337519A CN111397998A CN 111397998 A CN111397998 A CN 111397998A CN 202010337519 A CN202010337519 A CN 202010337519A CN 111397998 A CN111397998 A CN 111397998A
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- 239000012192 staining solution Substances 0.000 title claims abstract description 28
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 41
- DLFVBJFMPXGRIB-UHFFFAOYSA-N Acetamide Chemical compound CC(N)=O DLFVBJFMPXGRIB-UHFFFAOYSA-N 0.000 claims abstract description 34
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 34
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims abstract description 34
- 210000004027 cell Anatomy 0.000 claims abstract description 24
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 17
- 229960000304 folic acid Drugs 0.000 claims abstract description 17
- 235000019152 folic acid Nutrition 0.000 claims abstract description 17
- 239000011724 folic acid Substances 0.000 claims abstract description 17
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 12
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 12
- FBWADIKARMIWNM-UHFFFAOYSA-N N-3,5-dichloro-4-hydroxyphenyl-1,4-benzoquinone imine Chemical compound C1=C(Cl)C(O)=C(Cl)C=C1N=C1C=CC(=O)C=C1 FBWADIKARMIWNM-UHFFFAOYSA-N 0.000 claims abstract description 8
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims abstract description 4
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims abstract description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims abstract description 4
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000004327 boric acid Substances 0.000 claims abstract description 4
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 4
- 239000011734 sodium Substances 0.000 claims abstract description 4
- 239000011975 tartaric acid Substances 0.000 claims abstract description 4
- 235000002906 tartaric acid Nutrition 0.000 claims abstract description 4
- KSBAEPSJVUENNK-UHFFFAOYSA-L tin(ii) 2-ethylhexanoate Chemical compound [Sn+2].CCCCC(CC)C([O-])=O.CCCCC(CC)C([O-])=O KSBAEPSJVUENNK-UHFFFAOYSA-L 0.000 claims abstract description 4
- 238000010186 staining Methods 0.000 claims description 11
- 210000003701 histiocyte Anatomy 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 238000004043 dyeing Methods 0.000 abstract description 38
- 210000001519 tissue Anatomy 0.000 abstract description 18
- 230000035945 sensitivity Effects 0.000 abstract description 14
- 239000000243 solution Substances 0.000 abstract description 12
- 238000004040 coloring Methods 0.000 abstract description 6
- 210000000805 cytoplasm Anatomy 0.000 abstract description 4
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 abstract description 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 abstract description 2
- 210000002744 extracellular matrix Anatomy 0.000 abstract description 2
- 230000014759 maintenance of location Effects 0.000 abstract description 2
- 239000003002 pH adjusting agent Substances 0.000 abstract description 2
- 238000002360 preparation method Methods 0.000 abstract description 2
- 238000003756 stirring Methods 0.000 description 15
- 239000007788 liquid Substances 0.000 description 12
- 238000010438 heat treatment Methods 0.000 description 10
- 239000008367 deionised water Substances 0.000 description 5
- 229910021641 deionized water Inorganic materials 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 210000003802 sputum Anatomy 0.000 description 2
- 208000024794 sputum Diseases 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 1
- 206010019280 Heart failures Diseases 0.000 description 1
- 210000001132 alveolar macrophage Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 210000005087 mononuclear cell Anatomy 0.000 description 1
- 230000005937 nuclear translocation Effects 0.000 description 1
- 238000009595 pap smear Methods 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a staining solution for tissue cell slicing, which comprises the following components: a color-developing agent; dimethyl sulfoxide; polyethylene glycol; folic acid; a color hiding agent; an acetamide; a pH adjusting agent; water; wherein the pH regulator is one of acetic acid, citric acid, tartaric acid and boric acid; the color developing agent is one of dichlorophenol indophenol and dichlorophenol indophenol sodium; the color hiding agent is one of stannous octoate and zinc powder. Compared with the dyeing solution in the prior art, the preparation process of the dyeing solution simplifies the operation steps and greatly shortens the dyeing time; the sensitivity is high, the dyeing level is clear, the color is bright, the coloring is obvious, and the coloring color retention time is long; when in dyeing, the specificity is higher, the cytoplasm and the extracellular matrix are dyed, the gradation is rich, the coloring is clear, the gradation is clear, and the film reading is easy.
Description
Technical Field
The invention relates to a compound combination, in particular to a staining solution for tissue cell section.
Background
Tissue cells are also known as phagocytes. Mononuclear cells from blood are found in sputum, serosal cavity fluid and cervical smear.
The tissue cell shape is round, oval or various irregular shapes. The nucleus is circular, oval, oblong or kidney-shaped. Chromatin within nuclei has few and fine particles and is very pale in color. An unobtrusive kernel is sometimes visible. Nuclear translocation and many vacuoles in the cytoplasm are the main features. Papanicolaou staining cytoplasm is green, blue, or reddish. The tissue cells in the sputum engulf a number of carbon dust, called carbon dust cells. For example, cells containing ferrihemoxanthin are called ferrihemoxanthin cells, or heart failure cells.
At present, when the conventional histiocyte section is stained, staining solution is usually used for staining, and the conventional staining solution has the defects of unobvious staining and low sensitivity in the aspects of specificity and sensitivity.
Disclosure of Invention
The present invention is directed to provide a staining solution for tissue cell sections, which solves the problems of the background art described above.
In order to achieve the purpose, the invention provides the following technical scheme:
a staining solution for tissue cell section, the staining solution composition comprises the following components by weight percent: 0.05-0.25 part of color developing agent; 0.1-0.35 part of dimethyl sulfoxide; 0.05-0.25 part of polyethylene glycol; 5-9 parts of folic acid; 1-5 parts of a leuco agent; 15-19 parts of acetamide; 15-23 parts of a pH regulator; 43.15-63.8 parts of water.
As a further scheme of the invention: the dyeing liquid composition comprises the following components in percentage by weight: 0.1-0.2 parts of color developing agent; 0.2-0.3 part of dimethyl sulfoxide; 0.1-0.2 part of polyethylene glycol; 6-8 parts of folic acid; 2-4 parts of a color hiding agent; 16-18 parts of acetamide; 17-21 parts of a pH regulator; 48.3-58.6 parts of water.
As a further scheme of the invention: the dyeing liquid composition comprises the following components in percentage by weight: 0.15 part of color developing agent; 0.25 part of dimethyl sulfoxide; 0.15 part of polyethylene glycol; 7 parts of folic acid; 3 parts of a leuco agent; 17 parts of acetamide; 19 parts of a pH regulator; 53.45 parts of water.
As a further scheme of the invention: the pH regulator is one of acetic acid, citric acid, tartaric acid and boric acid.
As a further scheme of the invention: the color developing agent is one of dichlorophenol indophenol and dichlorophenol indophenol sodium.
As a further scheme of the invention: the color-masking agent is one of stannous octoate and zinc powder.
Compared with the prior art, the invention has the beneficial effects that:
1. compared with the dyeing solution in the prior art, the preparation process of the dyeing solution simplifies the operation steps and greatly shortens the dyeing time.
2. Compared with the dyeing solution in the prior art, the dyeing solution provided by the invention has the advantages that the sensitivity is higher, the dyeing level is clear, the color is bright, the coloring is obvious, and the coloring color retention time is longer.
3. Compared with the original staining solution, the staining solution has higher specificity during staining, stains cytoplasm and extracellular matrix, has rich layers, clear coloring and clear layers, and is easy to read.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
In the embodiment of the invention, the staining solution for the tissue cell section comprises a staining dye complex in percentage by weight
Comprises the following components: 0.05% of color developing agent; 0.1% of dimethyl sulfoxide; 0.05% of polyethylene glycol; 5% of folic acid; 1% of a leuco agent; 15% of acetamide; 15% of a pH regulator; and (5) 63.8 percent of water.
In the above embodiment, a method for preparing a staining solution for tissue cell section includes the following steps:
step one, adding folic acid and a color developing agent into deionized water, stirring and dissolving; when the room temperature is lower, the heating can be properly carried out, and the heating temperature is not more than 16 ℃;
adding a leuco agent, polyethylene glycol and dimethyl sulfoxide into the solution, and stirring for reaction;
adding a pH regulator and acetamide, regulating the pH value of the system, and stirring for reaction;
the dyeing liquid prepared in the example and the dyeing liquid in the prior art have the following index parameters:
| sensitivity of the probe | Degree of specificity | |
| Dyeing liquor for dyeing | 96.8 | 92.3 |
| Dyeing liquor of the prior art | 74.1 | 68.9 |
Remarking:
sensitivity is 100% of true positive people/(true positive people + false negative people)
The specificity is 100 percent of true negative people/(true negative people + false positive people)
Example 2
In the embodiment of the invention, the staining solution for the tissue cell section comprises a staining dye complex in percentage by weight
Comprises the following components: 0.1% of color developing agent; 0.2% of dimethyl sulfoxide; 0.1 percent of polyethylene glycol; 6% of folic acid; 2% of a leuco agent; 16% of acetamide; 17% of a pH regulator; 58.6 percent of water.
In the above embodiment, a method for preparing a staining solution for tissue cell section includes the following steps:
step one, adding folic acid and a color developing agent into deionized water, stirring and dissolving; when the room temperature is lower, the heating can be properly carried out, and the heating temperature is not more than 16 ℃;
adding a leuco agent, polyethylene glycol and dimethyl sulfoxide into the solution, and stirring for reaction;
adding a pH regulator and acetamide, regulating the pH value of the system, and stirring for reaction;
the dyeing liquid prepared in the example and the dyeing liquid in the prior art have the following index parameters:
| sensitivity of the probe | Degree of specificity | |
| Dyeing liquor for dyeing | 96.9 | 93.1 |
| Dyeing liquor of the prior art | 74.1 | 68.9 |
Remarking:
sensitivity is 100% of true positive people/(true positive people + false negative people)
The specificity is 100 percent of true negative people/(true negative people + false positive people)
Example 3
In the embodiment of the invention, the staining solution for the tissue cell section comprises a staining dye complex in percentage by weight
Comprises the following components: 0.15% of color developing agent; 0.25 percent of dimethyl sulfoxide; 0.15 percent of polyethylene glycol; 7% of folic acid; 3% of a leuco agent; 17% of acetamide; 19% of a pH regulator; 53.45 percent of water.
In the above embodiment, a method for preparing a staining solution for tissue cell section includes the following steps:
step one, adding folic acid and a color developing agent into deionized water, stirring and dissolving; when the room temperature is lower, the heating can be properly carried out, and the heating temperature is not more than 16 ℃;
adding a leuco agent, polyethylene glycol and dimethyl sulfoxide into the solution, and stirring for reaction;
adding a pH regulator and acetamide, regulating the pH value of the system, and stirring for reaction;
the dyeing liquid prepared in the example and the dyeing liquid in the prior art have the following index parameters:
| sensitivity of the probe | Degree of specificity | |
| Dyeing liquor for dyeing | 97.2 | 93.3 |
| Dyeing liquor of the prior art | 74.1 | 68.9 |
Remarking:
sensitivity is 100% of true positive people/(true positive people + false negative people)
The specificity is 100 percent of true negative people/(true negative people + false positive people)
Example 4
In the embodiment of the invention, the staining solution for the tissue cell section comprises a staining dye complex in percentage by weight
Comprises the following components: 0.2% of color developing agent; 0.3 percent of dimethyl sulfoxide; 0.2 percent of polyethylene glycol; 8% of folic acid; 4% of a leuco agent; 18% of acetamide; 21% of a pH regulator; 48.3 percent of water.
In the above embodiment, a method for preparing a staining solution for tissue cell section includes the following steps:
step one, adding folic acid and a color developing agent into deionized water, stirring and dissolving; when the room temperature is lower, the heating can be properly carried out, and the heating temperature is not more than 16 ℃;
adding a leuco agent, polyethylene glycol and dimethyl sulfoxide into the solution, and stirring for reaction;
adding a pH regulator and acetamide, regulating the pH value of the system, and stirring for reaction;
the dyeing liquid prepared in the example and the dyeing liquid in the prior art have the following index parameters:
| sensitivity of the probe | Degree of specificity | |
| Dyeing liquor for dyeing | 97.5 | 93.7 |
| Dyeing liquor of the prior art | 74.1 | 68.9 |
Remarking:
sensitivity is 100% of true positive people/(true positive people + false negative people)
The specificity is 100 percent of true negative people/(true negative people + false positive people)
Example 5
In the embodiment of the invention, the staining solution for the tissue cell section comprises a staining dye complex in percentage by weight
Comprises the following components: 0.25% of color developing agent; 0.35 percent of dimethyl sulfoxide; 0.25 percent of polyethylene glycol; 9% of folic acid; 5% of a leuco agent; 19% of acetamide; 23% of a pH regulator; 43.15 percent of water.
In the above embodiment, a method for preparing a staining solution for tissue cell section includes the following steps:
step one, adding folic acid and a color developing agent into deionized water, stirring and dissolving; when the room temperature is lower, the heating can be properly carried out, and the heating temperature is not more than 16 ℃;
adding a leuco agent, polyethylene glycol and dimethyl sulfoxide into the solution, and stirring for reaction;
adding a pH regulator and acetamide, regulating the pH value of the system, and stirring for reaction;
the dyeing liquid prepared in the example and the dyeing liquid in the prior art have the following index parameters:
| sensitivity of the probe | Degree of specificity | |
| Dyeing liquor for dyeing | 97.7 | 93.9 |
| Dyeing liquor of the prior art | 74.1 | 68.9 |
Remarking:
sensitivity is 100% of true positive people/(true positive people + false negative people)
The specificity is 100 percent of true negative people/(true negative people + false positive people)
Example 6
The pH adjusting agent described in examples 1 to 5 is one of acetic acid, citric acid, tartaric acid and boric acid.
Example 7
The color developer described in examples 1 to 5 is either dichlorophenol indophenol or dichlorophenol indophenol sodium.
In the case of the example 8, the following examples are given,
in the above examples 1 to 5, the leuco agent is one of stannous octoate and zinc powder.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (6)
1. A staining solution for tissue cell slicing is characterized in that the staining solution composition comprises the following components in percentage by weight: 0.05-0.25 part of color developing agent; 0.1-0.35 part of dimethyl sulfoxide; 0.05-0.25 part of polyethylene glycol; 5-9 parts of folic acid; 1-5 parts of a leuco agent; 15-19 parts of acetamide; 15-23 parts of a pH regulator; 43.15-63.8 parts of water.
2. The staining solution for tissue cell sections according to claim 1, wherein the staining complex comprises the following components in percentage by weight: 0.1-0.2 parts of color developing agent; 0.2-0.3 part of dimethyl sulfoxide; 0.1-0.2 part of polyethylene glycol; 6-8 parts of folic acid; 2-4 parts of a color hiding agent; 16-18 parts of acetamide; 17-21 parts of a pH regulator; 48.3-58.6 parts of water.
3. The staining solution for tissue cell sections according to claim 1, wherein the staining complex comprises the following components in percentage by weight: 0.15 part of color developing agent; 0.25 part of dimethyl sulfoxide; 0.15 part of polyethylene glycol; 7 parts of folic acid; 3 parts of a leuco agent; 17 parts of acetamide; 19 parts of a pH regulator; 53.45 parts of water.
4. A staining solution for histiocyte slices as claimed in any one of claims 1 to 3, characterized in that the pH regulator is one of acetic acid, citric acid, tartaric acid and boric acid.
5. The staining solution for histiocyte slices as claimed in any one of claims 1 to 3, wherein the color-developing agent is one of dichlorophenol indophenol and dichlorophenol indophenol sodium.
6. The staining solution for histiocyte slices as claimed in any one of claims 1 to 3, wherein the leuco agent is one of stannous octoate and zinc powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010337519.4A CN111397998A (en) | 2020-04-26 | 2020-04-26 | Staining solution for tissue cell section |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010337519.4A CN111397998A (en) | 2020-04-26 | 2020-04-26 | Staining solution for tissue cell section |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111397998A true CN111397998A (en) | 2020-07-10 |
Family
ID=71431686
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010337519.4A Pending CN111397998A (en) | 2020-04-26 | 2020-04-26 | Staining solution for tissue cell section |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100255571A1 (en) * | 2007-08-02 | 2010-10-07 | Daniel Groelz | Method and device for fixing/stabilising a sample |
| US20110236310A1 (en) * | 2008-12-25 | 2011-09-29 | Canon Kabushiki Kaisha | Labeling composition for intraocular tissue, labeling method of intraocular tissue, and screening method |
| CN103483872A (en) * | 2013-09-30 | 2014-01-01 | 青岛农业大学 | Staining agent combination for tumor tissue cell and preparation method thereof |
| US20140295404A1 (en) * | 2013-03-01 | 2014-10-02 | Andrew Simon Goldsborough | Sample fixation and stabilisation |
| CN110308031A (en) * | 2019-07-24 | 2019-10-08 | 广州翰德泽信医药科技有限公司 | A kind of stable fungi fluorescent staining liquid |
| CN110455602A (en) * | 2019-07-18 | 2019-11-15 | 浙江中法制药有限公司 | Specific stain liquid and its preparation method and application based on cast-off cells dyeing |
-
2020
- 2020-04-26 CN CN202010337519.4A patent/CN111397998A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100255571A1 (en) * | 2007-08-02 | 2010-10-07 | Daniel Groelz | Method and device for fixing/stabilising a sample |
| US20110236310A1 (en) * | 2008-12-25 | 2011-09-29 | Canon Kabushiki Kaisha | Labeling composition for intraocular tissue, labeling method of intraocular tissue, and screening method |
| US20140295404A1 (en) * | 2013-03-01 | 2014-10-02 | Andrew Simon Goldsborough | Sample fixation and stabilisation |
| CN103483872A (en) * | 2013-09-30 | 2014-01-01 | 青岛农业大学 | Staining agent combination for tumor tissue cell and preparation method thereof |
| CN110455602A (en) * | 2019-07-18 | 2019-11-15 | 浙江中法制药有限公司 | Specific stain liquid and its preparation method and application based on cast-off cells dyeing |
| CN110308031A (en) * | 2019-07-24 | 2019-10-08 | 广州翰德泽信医药科技有限公司 | A kind of stable fungi fluorescent staining liquid |
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