CN111358438A - Multi-wavelength laser diagnostic device - Google Patents
Multi-wavelength laser diagnostic device Download PDFInfo
- Publication number
- CN111358438A CN111358438A CN202010202878.9A CN202010202878A CN111358438A CN 111358438 A CN111358438 A CN 111358438A CN 202010202878 A CN202010202878 A CN 202010202878A CN 111358438 A CN111358438 A CN 111358438A
- Authority
- CN
- China
- Prior art keywords
- light
- light source
- optical signal
- optical
- splitting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000003287 optical effect Effects 0.000 claims abstract description 118
- 238000012545 processing Methods 0.000 claims abstract description 58
- 230000005284 excitation Effects 0.000 claims abstract description 56
- 238000004458 analytical method Methods 0.000 claims abstract description 17
- 239000013307 optical fiber Substances 0.000 claims description 20
- 239000000758 substrate Substances 0.000 claims description 19
- 238000001914 filtration Methods 0.000 claims description 11
- 230000005540 biological transmission Effects 0.000 claims description 9
- 230000007246 mechanism Effects 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 7
- 238000003745 diagnosis Methods 0.000 abstract description 18
- 238000000034 method Methods 0.000 abstract description 9
- 230000009286 beneficial effect Effects 0.000 abstract description 7
- 230000008569 process Effects 0.000 abstract description 6
- 238000000338 in vitro Methods 0.000 abstract description 4
- 238000011282 treatment Methods 0.000 abstract description 4
- 238000001727 in vivo Methods 0.000 abstract description 2
- 206010028980 Neoplasm Diseases 0.000 description 9
- 239000003504 photosensitizing agent Substances 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 210000004881 tumor cell Anatomy 0.000 description 3
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000002428 photodynamic therapy Methods 0.000 description 2
- 238000004611 spectroscopical analysis Methods 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 238000011369 optimal treatment Methods 0.000 description 1
- 238000006552 photochemical reaction Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/10—Beam splitting or combining systems
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2562/00—Details of sensors; Constructional details of sensor housings or probes; Accessories for sensors
- A61B2562/02—Details of sensors specially adapted for in-vivo measurements
- A61B2562/0233—Special features of optical sensors or probes classified in A61B5/00
- A61B2562/0238—Optical sensor arrangements for performing transmission measurements on body tissue
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Biomedical Technology (AREA)
- Medical Informatics (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Heart & Thoracic Surgery (AREA)
- Public Health (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Optics & Photonics (AREA)
- General Physics & Mathematics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a multi-wavelength laser diagnosis device, which comprises a light source device, a light splitting device and a processing device, wherein the light splitting device is used for splitting light beams; the light source device is used for outputting light emitted by the excitation light source and the cold light source and collecting a mixed light signal containing reflected light and fluorescence; the optical splitting device is used for acquiring the mixed optical signal to perform optical splitting analysis and outputting a target optical signal; the processing device is used for acquiring a target optical signal output by the light splitting device, processing the target optical signal and generating a target image and/or an analysis result; the invention can irradiate the biological tissue in vivo through the excitation light source and the cold light source to obtain the mixed light signal of the reflected light and the fluorescence on the premise of not increasing the size and the volume of the part of the existing equipment entering the body, and obtains the pure fluorescence and the background light (namely the original mixed light signal or the mixed light signal filtered by a small amount of stray light) by the light splitting treatment in vitro, the processing device processes and generates a clearer and more reliable diagnostic image, provides more accurate data base for the medical work, and is beneficial to reducing the misdiagnosis rate in the subsequent medical work.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a multi-wavelength laser diagnosis device.
Background
Photonics has driven the development of biology and medicine, and photodynamic diagnosis and photodynamic therapy of cancer have become important topics in the fields of information science and life science research. The photodynamic diagnosis refers to the diagnosis of cancer by using the characteristic spectrum of a photosensitizer having affinity for tumors under the action of light with specific wavelength; the photodynamic therapy is that a photosencitizer is needed during the therapy, and the therapeutic effect is generated by the activation of photons of visible light (400-700 nm); a novel method for treating tumors by exciting a photosensitizer in a target cell with light of a specific wavelength under aerobic conditions. The method is characterized in that tumor tissues can selectively absorb a photosensitizer, then the lesion part is irradiated by light with specific wavelength, so that the photosensitizer in the tumor tissues generates violent photochemical reaction, mitochondrial molecules in tumor cells are oxidized into singlet oxygen, and the singlet oxygen causes an excitation reaction, thereby selectively killing the cancer cells.
In the traditional photodynamic diagnosis and treatment process, the common means also comprises fluorescence localization, the photosensitizer is selectively accumulated in the tumor tissue by utilizing the affinity of the tumor tissue to the photosensitizer, a significant concentration difference is formed between the lesion tissue and the normal tissue after a period of time, the lesion tissue emits fluorescence with a specific wavelength under the irradiation of exciting light with a specific wavelength, and the normal tissue does not have the absorption peak or has a very weak absorption peak.
Medical optical imaging generally employs a cold light source, i.e., a light source that emits light with little infrared spectrum; in practical applications, however, the wavelengths of the laser light for therapy or the visible light for imaging (especially red light) and the small amount of infrared light mixed in are long, and the fluorescence is easily covered, as shown in fig. 4; in addition, for fluorescence, the luminescence process is almost immediate after the excitation light stops (10)-9-10-6s) stopping, the fluorescence decays quickly; therefore, in the actual diagnosis process, the obtained diagnosis image is not accurate enough, and then the diagnosis result has judgment error, even the optimal treatment time is delayed, and tragedy occurs.
At present, most of photodynamic diagnosis medical equipment adopts an optical fiber and endoscope structure to act on a human body to obtain a diagnosis image, so that the problem that how to pursue the accuracy of data and not increase the volume of the equipment to cause more discomfort of the human body is solved.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides the multi-wavelength laser diagnosis device which is beneficial to obtaining more accurate optical images and further provides more effective data for medical work.
In order to achieve the purpose, the technical scheme of the invention comprises the following steps:
in a first aspect, the present invention provides a multi-wavelength laser diagnostic apparatus, including a light source apparatus, a light splitting apparatus, and a processing apparatus;
the light source device is used for outputting light emitted by the excitation light source and the cold light source and collecting a mixed light signal containing reflected light and fluorescence;
the optical splitting device is used for acquiring the mixed optical signal, splitting the mixed optical signal and analyzing the mixed optical signal to output a target optical signal;
and the processing device is used for acquiring the target optical signal output by the light splitting device and processing the target optical signal to generate a target image and/or an analysis result.
Further, in the above multi-wavelength laser diagnostic apparatus, the light source device includes a multi-wavelength excitation light source, a cold light source, an optical fiber sensor and a pilot part, and an end face of the pilot part is at least provided with an objective lens and a light guide window; the optical fiber sensor is arranged in the guide part in the coaxial direction of the objective lens; the guide part is communicated to the multi-wavelength excitation light source, the cold light source and the optical signal receiving of the light splitting device through an optical fiber transmission line; the processing device is electrically and/or communicatively connected with the multi-wavelength excitation light source and the cold light source so as to control the on and/or off of the multi-wavelength excitation light source and the cold light source.
Further, in the multi-wavelength laser diagnostic apparatus, the optical splitter includes a spectrometer, and the spectrometer includes an optical signal receiving end, a first optical splitting channel and a second optical splitting channel; and a filtering membrane/filtering mirror is arranged between the optical signal receiving end and the first light splitting channel, the output ends of the two light splitting channels are correspondingly provided with optical detectors, and the signal output ends of the optical detectors are connected with the processing device through circuits.
Further, in the multi-wavelength laser diagnosis device, the processing device is further configured to preset irradiation time of the excitation light source and/or preset irradiation times of excitation lasers with different powers emitted by the excitation light source.
Further, in the above multi-wavelength laser diagnostic apparatus, an aperture is disposed in the pilot portion between the objective lens and the optical fiber sensor, and the aperture is electrically connected to the control device.
Further, in the multi-wavelength laser diagnostic apparatus, the multi-wavelength excitation light source includes a fixing plate and at least one LED light-emitting substrate fixed on the fixing plate; a focusing assembly is arranged between the multi-wavelength excitation light source and the optical fiber transmission line; the control device drives the actuating mechanism to drive the fixing plate to move through the driving mechanism, so that the central point of the emitted light of any LED light-emitting substrate on the fixing plate moves to the incident light axis of the optical focusing assembly.
Further, in the above multi-wavelength laser diagnostic apparatus, the focusing assembly includes a first optical lens, a second optical lens, a focusing collimating lens and a heat insulating sheet, wherein the light beam emitted from the LED light emitting substrate can pass through the heat insulating sheet, be sequentially shaped by the first optical lens, be focused by the second optical lens onto the focusing collimating lens, and be output to the pilot portion.
In a second aspect, the present invention further provides a processing apparatus, including a processor and a memory electrically connected to the processor, where the memory stores a program, and when the program is executed by the processor, the processing apparatus executes:
controlling the light source device to output light emitted by the excitation light source and the cold light source and collect a mixed light signal containing reflected light and fluorescence;
controlling a light splitting device to obtain the mixed optical signal for light splitting analysis to obtain a target optical signal;
and generating a target image and/or an analysis result according to the target optical signal processing.
Further, in the processing device described above, the program executes: controlling a light splitting device to obtain the mixed optical signal for light splitting analysis to obtain a target optical signal, comprising
Controlling a light splitting device to split the mixed optical signal into two same parts, wherein one part of light is filtered by a filter membrane/filter mirror to filter reflected light in the mixed optical signal to obtain a fluorescent signal;
the other part of the light is directly output or output after filtering out the stray light through a filter with different wavelengths.
The invention has the beneficial effects that:
the multi-wavelength laser diagnosis device can irradiate internal biological tissues by controlling an external excitation light source, a cold light source and the like through the processing device on the premise of not increasing the indexes such as the size, the volume and the like of the part (namely a guide part connected with a lead) of the existing equipment entering the body, obtains internal reflection light and fluorescence mixed light signals, performs light splitting treatment in vitro to obtain simple fluorescence and background light (namely original mixed light signals or mixed light signals filtered by a small amount of stray light), generates a clearer and more reliable diagnosis image through image processing of the processing device, provides a more accurate data base for medical work, and is beneficial to reducing misdiagnosis rate in subsequent medical work.
Drawings
In order to more clearly illustrate the detailed description of the invention or the technical solutions in the prior art, the drawings that are needed in the detailed description of the invention or the prior art will be briefly described below. Throughout the drawings, like elements or portions are generally identified by like reference numerals. In the drawings, elements or portions are not necessarily drawn to scale.
FIG. 1 is a schematic structural diagram of an embodiment of the multi-wavelength laser diagnostic apparatus according to the present invention;
FIG. 2 is a schematic structural view of the pilot section shown in FIG. 1;
FIG. 3 is a schematic structural view of the fixing plate shown in FIG. 1;
FIG. 4 is a diagram illustrating the overlaying of reflected light with fluorescence in the prior art;
FIG. 5 is a schematic diagram of the present invention to obtain optical signals with distinct wavelength differences;
fig. 6 is a schematic structural diagram of the light-gathering component of the present invention.
In the drawings, there is shown in the drawings,
1-a control device; 2-a multi-wavelength excitation light source; 21-fixing the plate; 22-a light-emitting substrate; 23-a focusing assembly; 3-a cold light source; 32-a first optical lens; 33-second optical lens, 34-focusing collimating lens; 4-a pilot section; 40-a fiber optic sensor; 41-objective lens; 42-a light guide window; 31-a thermal insulation sheet; 5-a drive device; 6-driving the actuator; 7-a spectrometer; 71-optical signal receiving end; 72-a first light splitting channel; 73-a second drop channel; 74-filter membrane/filter lens; 75-a light detector; 76-light detector.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains.
As shown in fig. 1-3, a multi-wavelength laser diagnostic apparatus includes a light source device, a light splitting device, and a processing device;
the light source device is used for outputting light emitted by the excitation light source and the cold light source and collecting a mixed light signal containing reflected light and fluorescence;
the optical splitting device is used for acquiring the mixed optical signal, splitting the mixed optical signal and analyzing the mixed optical signal to output a target optical signal;
and the processing device is used for acquiring the target optical signal output by the light splitting device and processing the target optical signal to generate a target image and/or an analysis result.
The device of the invention divides the mixed light signal containing multi-wavelength light into reflected light and fluorescence by means of internal irradiation, collection of mixed light signal and in-vitro spectroscopic analysis, and selectively filters to obtain the required image for diagnosis.
Specifically, in the invention, the excitation light source is used for emitting excitation laser to act on biological tissues to generate fluorescence, and the wavelength range of the excitation light source is 250nm-2500 nm; the cold light source is used for emitting illumination light, and the wavelength range of the cold light source is 350-760 nm.
In one embodiment of the present invention, the light source device includes a multi-wavelength excitation light source 2, a cold light source 3, an optical fiber sensor 40 and a pilot part 4, wherein an end face of the pilot part 4 is at least provided with an objective lens 41 and a light guide window 42; the optical fiber sensor 40 is arranged in the guide part 4 in the coaxial direction of the objective lens 41; the guide part 4 is communicated to the multi-wavelength excitation light source 2, the cold light source 3 and the optical signal receiving end 71 of the light splitting device through optical fiber transmission lines; the processing device 1 is electrically and/or communicatively connected with the multi-wavelength excitation light source 2 and the cold light source 3 to control the on and/or off of the multi-wavelength excitation light source 2 and the cold light source 3.
A focusing assembly 23 is arranged between the multi-wavelength excitation light source 2 and the optical fiber transmission line, so that excitation laser of the multi-wavelength excitation light source 2 is shaped and focused by the focusing assembly 23 and then transmitted to the light guide window; the multi-wavelength excitation light source 2 and the cold light source 3 respectively correspond to two different light guide windows on the guide part to output different lights.
The optical splitting device comprises a spectrometer 7, and the spectrometer 7 comprises an optical signal receiving end 71, a first optical splitting channel 72 and a second optical splitting channel 73; a filtering film/filtering mirror 74 is arranged between the optical signal receiving end 71 and the first light splitting channel 72, the output ends of the two light splitting channels (72, 73) are respectively and correspondingly provided with optical detectors (75,76), and the signal output ends of the optical detectors (75,76) are in circuit connection with the processing device.
The light signal receiving end 71 of the spectrometer 7 is connected to the pilot unit 4 via an optical fiber transmission line to receive the mixed light signal collected by the light source device for spectroscopic analysis, and the purpose of the spectrometer is to separate the fluorescence and reflected light in the mixed light signal, as shown in fig. 5, for generating a clearer and more reliable optical diagnostic image. The optical signal output end of the spectrometer converts the target optical signal into an electric signal through the optical detector and transmits the electric signal to the signal receiving port of the processing device, the processing device is an image processor realized through a single chip microcomputer, and after the target optical signal output by the spectrometer is obtained, calculation processing is carried out to obtain an optical diagnosis image or analysis data.
For this purpose, the optical signal receiving end 71 of the spectrometer 7 divides the optical signal into two identical parts, one part enters the first light splitting channel 72 and passes through the filtering membrane/filtering mirror 74 to filter out the reflected light, so that the optical detector 75 corresponding to the output end is only sensitive to fluorescence; the other part of light enters the second light splitting channel 73, a small amount of stray light existing in practical application can be filtered by adopting optical filters with different wavelengths, or optical filters are not adopted, and then the electric signal is output to the processing device 1 through photoelectric conversion of the optical detector 76; the processing device 1 can acquire a simple fluorescence signal and mixed light, and perform comprehensive processing imaging to obtain a clear fluorescence diagnostic image.
When the method works, firstly, the fluorescent agent is injected into biological tissues (such as the surface layer of viscera), and the fluorescent agent is enriched in a tumor cell area by utilizing the affinity characteristic of tumor cells to the fluorescent agent; then the pilot part enters into the organism, the processing device controls the excitation light source to emit excitation laser to irradiate the organism tissue, and the fluorescent agent absorbs the excitation laser to generate a fluorescence phenomenon; wherein the fluorescence wavelength can be directionally controlled by controlling the wavelength of the excitation laser; then the processing device controls the light source device to output illumination light (generated by a cold light source), and collects the current mixed light signal of the biological tissue through the optical fiber sensor, wherein the mixed light signal comprises emission light and fluorescence; the mixed optical signal collected by the optical fiber sensor is transmitted to a spectrometer outside a living body through an optical fiber transmission line, the mixed optical signal is subjected to light splitting analysis through the spectrometer to obtain a target optical signal, the target optical signal is subjected to photoelectric conversion through an optical detector and then an electric signal is output to a processing device for calculation processing, and an image and/or an analysis result for optical diagnosis is obtained.
Furthermore, in order to improve the accuracy of the optical image, the processing device 1 presets the irradiation time of the excitation light source and/or presets the irradiation times of the excitation light source to emit excitation lasers with different powers, and increases the fluorescence intensity by increasing the irradiation time of the excitation lasers with multiple wavelengths and/or adopting the excitation lasers with different powers to irradiate for multiple times, so that the difference between fluorescence and reflected light is increased in the process of acquiring the optical signal by the optical sensor, and a clearer and more accurate optical image is acquired.
Furthermore, in the device, an aperture (not marked in the figure) is arranged at a position between the objective lens 41 and the optical fiber sensor 40 in the guide part 4, and the aperture is electrically connected with the processing device; the size of the aperture is adjusted by the driving of the processing device 1, then the intensity of the acquired optical signal is enhanced by enlarging the aperture, the exposure time is reduced by controlling the exposure degree to be minimized, the processing device is facilitated to acquire a clearer optical image, and a fluorescence area is distinguished.
The multi-wavelength excitation light source is beneficial to generating excitation laser with proper wavelength according to actual needs, promotes the wavelength difference of subsequent reflected light and fluorescence, and is beneficial to distinguishing by a spectrometer. Specifically, in an optional embodiment, as shown in fig. 3, the multi-wavelength excitation light source includes a fixing plate 21 and at least one LED light-emitting substrate 22 fixed on the fixing plate 21, each LED light-emitting substrate 22 may be packaged with a single LED chip, or may be composed of a plurality of LED chip arrays with the same wavelength or different wavelengths, the wavelength range of the LED chip is generally selected from 250nm to 2500nm, the LED chip may also be a white LED chip with a color temperature of 6000 and 6500k, here, the LED light-emitting substrates 12 with various wavelength forms may be provided according to requirements, and the LED light-emitting substrates 22 are arranged on the fixing plate 21 in a certain manner. The processing device 1 drives the actuating mechanism 6 (i.e. the transmission switching mechanism) to drive the fixing plate 21 to move through the driving mechanism 5 (generally, driven by a motor), so that the central point of the emitted light of any one of the LED light-emitting substrates 22 on the fixing plate 21 moves to the incident optical axis of the optical focusing assembly 23, and the light beam of the LED light-emitting substrate 22 is output to the light guide window of the pilot portion 4 through the shaping and focusing of the optical focusing assembly 23.
As shown in fig. 6, the focusing assembly 23 includes a first optical lens 32, a second optical lens 33, a focusing collimating lens 34 and a heat insulating sheet 31, wherein the light beam emitted from the LED light-emitting substrate 22 passes through the heat insulating sheet 31, is sequentially shaped by the first optical lens 32, is focused by the second optical lens 33 onto the focusing collimating lens 34, and is output to the pilot portion 4.
The processing device 1 controls the actuating mechanism 6 to drive the multi-wavelength excitation light source 2 to move to a required position through the driving mechanism 5. In the present embodiment, a plurality of LED light-emitting substrates 22 with different wavelengths are all fixed on the front surface of the fixing plate 21, the optical focusing assembly 23 faces the front surface of the fixing plate 21, and the incident light axis of the optical focusing assembly is perpendicular to the front surface of the fixing plate 21, so that the central point of the emitted light of any LED light-emitting substrate 22 on the optical focusing assembly 23 can move to the incident light axis of the optical focusing assembly 23 as long as the actuator 6 drives the fixing plate 21 to move in the plane where the fixing plate is located.
In practice, the LED light-emitting substrates 22 are arranged in a regular matrix, which is beneficial to the processing apparatus 1 to optimize the motion trajectory of the actuator. As an example of this embodiment, the fixing plate 21 is square, and four LED light emitting substrates 22 with different wavelengths are fixed on the fixing plate, the wavelengths of the LED chips packaged on the four LED light emitting substrates 22 are respectively 375nm purple light, 470nm blue light, 630nm red light and 670nm red light, and the maximum power is 10-100W, so that the multi-wavelength excitation light source 2 can provide four LED light sources with different wavelengths.
The device can irradiate the biological tissue in vivo by controlling an external excitation light source, a cold light source and the like through the processing device on the premise of not increasing the indexes such as the size, the volume and the like of the part (namely a guide part connected with a lead) entering the body of the existing equipment, obtains the mixed light signal of internal reflection light and fluorescence, performs light splitting treatment in vitro to obtain simple fluorescence and background light (namely an original mixed light signal or a mixed light signal filtered with a small amount of stray light), generates a clearer and more reliable diagnostic image through image processing of the processing device, provides a more accurate data base for medical work, and is favorable for reducing the misdiagnosis rate in subsequent medical work.
It should be noted that the power supply device of the present invention can be implemented by using an external power supply, which is a mature technology in the field and will not be described in detail. The information or control command input components (such as keyboard) required by the processing device are all common knowledge in the art and are not described in detail.
In a second aspect, the present invention further provides a processing apparatus, including a processor and a memory electrically connected to the processor, where the memory stores a program, and when the program is executed by the processor, the processing apparatus executes:
controlling the light source device to output light emitted by the excitation light source and the cold light source and collect a mixed light signal containing reflected light and fluorescence;
controlling a light splitting device to obtain the mixed optical signal for light splitting analysis to obtain a target optical signal;
and generating a target image and/or an analysis result according to the target optical signal processing.
Further, in the processing device described above, the program executes: controlling a light splitting device to obtain the mixed optical signal for light splitting analysis to obtain a target optical signal, comprising
Controlling a light splitting device to split the mixed optical signal into two same parts, wherein one part of light is filtered by a filter membrane/filter mirror to filter reflected light in the mixed optical signal to obtain a fluorescent signal;
the other part of the light is directly output or output after filtering out the stray light through a filter with different wavelengths.
The processing device in this embodiment is used to implement the operation of the processing device in the above diagnostic device, and therefore, the operation principle and process thereof can be referred to the description of embodiment 1 of the present invention.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
Claims (9)
1. A multi-wavelength laser diagnostic device characterized by: comprises a light source device, a light splitting device and a processing device;
the light source device is used for outputting light emitted by the excitation light source and the cold light source and collecting a mixed light signal containing reflected light and fluorescence;
the optical splitting device is used for acquiring the mixed optical signal, splitting the mixed optical signal and analyzing the mixed optical signal to output a target optical signal;
and the processing device is used for acquiring the target optical signal output by the light splitting device and processing the target optical signal to generate a target image and/or an analysis result.
2. The multiwavelength laser diagnostic device according to claim 1, wherein: the light source device comprises a multi-wavelength excitation light source, a cold light source, an optical fiber sensor and a pilot part, wherein the end face of the pilot part is at least provided with an objective lens and a light guide window; the optical fiber sensor is arranged in the guide part in the coaxial direction of the objective lens; the guide part is communicated to the multi-wavelength excitation light source, the cold light source and the optical signal receiving of the light splitting device through an optical fiber transmission line; the processing device is electrically and/or communicatively connected with the multi-wavelength excitation light source and the cold light source so as to control the on and/or off of the multi-wavelength excitation light source and the cold light source.
3. The multiwavelength laser diagnostic apparatus according to claim 2, wherein: the spectrometer comprises an optical signal receiving end, a first light splitting channel and a second light splitting channel; and a filtering membrane/filtering mirror is arranged between the optical signal receiving end and the first light splitting channel, the output ends of the two light splitting channels are correspondingly provided with optical detectors, and the signal output ends of the optical detectors are connected with the processing device through circuits.
4. The multiwavelength laser diagnostic apparatus according to claim 2, wherein: the processing device is also used for presetting the irradiation time of the excitation light source and/or presetting the irradiation times of the excitation light source for emitting excitation laser with different powers.
5. The multiwavelength laser diagnostic apparatus according to claim 2, wherein: and a diaphragm is arranged at a position between the objective lens and the optical fiber sensor in the pilot part and is electrically connected with the control device.
6. The multiwavelength laser diagnostic device according to any one of claims 2 to 5, wherein: the multi-wavelength excitation light source comprises a fixing plate and at least one LED light-emitting substrate fixed on the fixing plate; a focusing assembly is arranged between the multi-wavelength excitation light source and the optical fiber transmission line; the control device drives the actuating mechanism to drive the fixing plate to move through the driving mechanism, so that the central point of the emitted light of any LED light-emitting substrate on the fixing plate moves to the incident light axis of the optical focusing assembly.
7. The multiwavelength laser diagnostic apparatus according to claim 6, wherein the focusing assembly comprises a first optical lens, a second optical lens, a focusing collimating lens and a heat insulating sheet, wherein the light beam emitted from the LED light emitting substrate can pass through the heat insulating sheet, be sequentially shaped by the first optical lens, be focused by the second optical lens to the focusing collimating lens and be output to the pilot portion.
8. A processing apparatus, comprising a processor and a memory electrically connected to the processor, wherein the memory stores a program, and when the program is executed by the processor, the program performs:
controlling the light source device to output light emitted by the excitation light source and the cold light source and collect a mixed light signal containing reflected light and fluorescence;
controlling a light splitting device to obtain the mixed optical signal for light splitting analysis to obtain a target optical signal;
and generating a target image and/or an analysis result according to the target optical signal processing.
9. The processing apparatus according to claim 8, wherein the program executes: controlling a light splitting device to obtain the mixed optical signal for light splitting analysis to obtain a target optical signal, comprising
Controlling a light splitting device to split the mixed optical signal into two same parts, wherein one part of light is filtered by a filter membrane/filter mirror to filter reflected light in the mixed optical signal to obtain a fluorescent signal;
the other part of the light is directly output or output after filtering out the stray light through a filter with different wavelengths.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010202878.9A CN111358438A (en) | 2020-03-20 | 2020-03-20 | Multi-wavelength laser diagnostic device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010202878.9A CN111358438A (en) | 2020-03-20 | 2020-03-20 | Multi-wavelength laser diagnostic device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111358438A true CN111358438A (en) | 2020-07-03 |
Family
ID=71198759
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010202878.9A Pending CN111358438A (en) | 2020-03-20 | 2020-03-20 | Multi-wavelength laser diagnostic device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111358438A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025082342A1 (en) * | 2023-10-19 | 2025-04-24 | 上海光声制药有限公司 | Integrated photodynamic diagnosis and treatment method |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030078477A1 (en) * | 2001-10-18 | 2003-04-24 | Korea Electrotechnology Research Institute | Fluorescence endoscope apparatus and method for imaging tissue within a body using the same |
| CN101238974A (en) * | 2007-02-05 | 2008-08-13 | 三洋电机株式会社 | camera device |
| CN101278829A (en) * | 2008-05-26 | 2008-10-08 | 上海理工大学 | Portable In Vivo Flow Cytometry |
| CN101869741A (en) * | 2010-06-29 | 2010-10-27 | 深圳市雷迈科技有限公司 | Multipurpose medical light source system |
| CN101874914A (en) * | 2010-06-29 | 2010-11-03 | 深圳市雷迈科技有限公司 | Positioning system for photodynamic therapy and fluorescence diagnosis |
| US20120248333A1 (en) * | 2011-03-30 | 2012-10-04 | Johannes Fallert | Device For Fluorescence Diagnosis |
| CN110031865A (en) * | 2019-05-14 | 2019-07-19 | 武汉大学 | A kind of vegetation detection binary channels fluorescent laser radar system |
-
2020
- 2020-03-20 CN CN202010202878.9A patent/CN111358438A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030078477A1 (en) * | 2001-10-18 | 2003-04-24 | Korea Electrotechnology Research Institute | Fluorescence endoscope apparatus and method for imaging tissue within a body using the same |
| CN101238974A (en) * | 2007-02-05 | 2008-08-13 | 三洋电机株式会社 | camera device |
| CN101278829A (en) * | 2008-05-26 | 2008-10-08 | 上海理工大学 | Portable In Vivo Flow Cytometry |
| CN101869741A (en) * | 2010-06-29 | 2010-10-27 | 深圳市雷迈科技有限公司 | Multipurpose medical light source system |
| CN101874914A (en) * | 2010-06-29 | 2010-11-03 | 深圳市雷迈科技有限公司 | Positioning system for photodynamic therapy and fluorescence diagnosis |
| US20120248333A1 (en) * | 2011-03-30 | 2012-10-04 | Johannes Fallert | Device For Fluorescence Diagnosis |
| CN110031865A (en) * | 2019-05-14 | 2019-07-19 | 武汉大学 | A kind of vegetation detection binary channels fluorescent laser radar system |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025082342A1 (en) * | 2023-10-19 | 2025-04-24 | 上海光声制药有限公司 | Integrated photodynamic diagnosis and treatment method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100853655B1 (en) | Device, light source system and method of use for optical diagnosis and treatment of skin diseases | |
| CN101874914B (en) | Positioning system for photodynamic therapy and fluorescence diagnosis | |
| US9486640B2 (en) | System and method for therapy and diagnosis comprising optical components for distribution of radiation | |
| US8046055B2 (en) | Lymph node detector | |
| JP4406091B2 (en) | Photodynamic processing apparatus for living organisms and living organs | |
| US8998468B2 (en) | Solid state light source with hybrid optical and electrical intensity control | |
| CN101869741B (en) | Multipurpose medical light source system | |
| WO2003068064A1 (en) | Cancer detection and adaptive dose optimization treatment system | |
| US20080269576A1 (en) | Light Source for Fluorescence Diagnosis and Photodynamic Therapy | |
| CN115316959B (en) | A three-color multi-channel optical fiber brain information recording system | |
| CN201727851U (en) | Photodynamic therapy and fluorescent diagnostic positioning system | |
| CN201731334U (en) | Multi-purpose medical light-source system | |
| CN111358438A (en) | Multi-wavelength laser diagnostic device | |
| CN111249625A (en) | Medical light source device and system | |
| CN114199849A (en) | A Fluorescence Optical Path System Applied in Fluorescence Imaging | |
| EP3942994B1 (en) | Light source device | |
| KR100536882B1 (en) | System for photodynamic therapy of living organisms and their organs and/or tissues | |
| WO2024227348A1 (en) | Optical system integrating optogenetic stimulation and optical signal detection, and imaging apparatus | |
| CN116392723A (en) | Photodynamic therapeutic apparatus | |
| RU2221605C2 (en) | Apparatus for luminescent diagnostics and photodynamic therapy | |
| RU2003126738A (en) | DEVICE AND METHOD FOR DIAGNOSTICS AND PHOTODYNAMIC THERAPY OF EYE DISEASES | |
| RU2649211C2 (en) | Automated laser complex for diagnosis and treatment of diseases by photodynamic therapy in oncology | |
| CN116602626B (en) | Parathyroid gland identification and blood supply detection device and method based on spectrometer | |
| HK40063267B (en) | Light source device | |
| HK40049047A (en) | Light source device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200703 |