CN111328770A - Indoor large-scale breeding method for nakayata nakamura - Google Patents
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- 238000011081 inoculation Methods 0.000 claims abstract description 63
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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Abstract
发明涉及昆虫饲养领域,具体公开了长尾潜蝇茧蜂的室内规模化繁育方法,包括以下步骤:将羽化的长尾潜蝇茧蜂置于养蜂笼中饲养;将装有2~3日龄橘小实蝇幼虫和幼虫饲料的接种盒放入养蜂笼内进行接种;将更换出的接种盒内橘小实蝇幼虫继续饲养,待幼虫发育至7日龄时,移至化蛹箱中让幼虫化蛹,化蛹10~14日收集剩下未羽化的蛹,即为寄生蛹;将寄生蛹放置养蜂笼中,至长尾潜蝇茧蜂羽化,即得长尾潜蝇茧蜂。本发明的长尾潜蝇茧蜂的室内规模化繁育方法简单易行,可在室内操作,能够大规模繁殖长尾潜蝇茧蜂,实现该茧蜂的终年不间断繁殖,可以向瓜果种植区提供大量的虫源,用于防治危害瓜果作物的橘小实蝇。The invention relates to the field of insect breeding, and specifically discloses an indoor large-scale breeding method of L. long-tailed wasp, comprising the following steps: placing the emerging L. long-tailed wasp in a beekeeping cage for rearing; The inoculation box of the old tangerine fruit fly larvae and the larval feed is put into the beekeeping cage for inoculation; the tangerine fruit fly larvae in the replaced inoculation box will continue to be raised, and when the larvae develop to 7 days of age, move to the pupation box The larvae are allowed to pupate in the middle, and the remaining pupas that do not emerge from the pupation are collected on the 10th to 14th day, and are the parasitic pupa; the parasitic pupa is placed in the beekeeping cage, to the eclosion of L. long-tailed cocoon, that is, the cocoon of L. long-tailed bee. The indoor large-scale breeding method of L. long-tailed wasp of the invention is simple and easy to operate, can be operated indoors, can breed L. long-tailed wasp on a large scale, realizes the uninterrupted reproduction of the wasp all year round, and can be planted on melons and fruits The area provides a large number of insect sources for the control of fruit flies that harm melon and fruit crops.
Description
技术领域technical field
本发明属于昆虫饲养领域,特别涉及一种长尾潜蝇茧蜂的室内规模化繁育方法。The invention belongs to the field of insect breeding, and in particular relates to an indoor large-scale breeding method of Liriomyza longiformis.
背景技术Background technique
橘小实蝇是一种世界性分布的瓜果类植物的重要害虫,能够取食危害300多种栽培果蔬作物和野生植物,橘小实蝇寄主广、传播快、危害隐蔽、世代重叠等特点,给农业生产和农产品贸易造成巨大的危害和经济损失。目前对橘小实蝇的防治主要依靠大量施用化学农药,长期采用化学农药防治会引发橘小实蝇抗药性增强、果蔬中的农药残留量增加、环境污染日趋严重等重大问题。采生物防治是一种经济、安全、持续和有效的防治橘小实蝇的方法,寄生蜂是实蝇类害虫的重要天敌类群,在害虫的生物防治中占据重要的地位。长尾潜蝇茧蜂,属膜翅目,茧蜂科,潜蝇茧蜂亚科,闭口茧蜂属,又名长尾全裂茧蜂,是防治橘小实蝇、瓜实蝇等实蝇类害虫的一种重要的寄生性天敌。长尾潜蝇茧蜂对多种经济重要性实蝇都可以寄生,例如橘小实蝇、瓜实蝇油、橄榄实蝇等,长尾潜蝇茧蜂寄生在实蝇幼虫,通过产卵于实蝇幼虫体内,卵孵化后在寄主体内生活,破坏寄主的生活史,从而最终导致寄主的死亡。在拉丁美洲和美国南部地区长尾潜蝇茧蜂已被广泛运用于果实蝇属的生物防治,而解决长尾潜蝇茧蜂的室内扩繁问题是长尾潜蝇茧蜂在田间得以利用实现生物防治的关键环节。实现长尾潜蝇茧蜂的室内规模化繁育对于有效利用长尾潜蝇茧蜂进行害虫生物防治具有重要意义。The fruit fly is an important pest of melon and fruit plants distributed worldwide. It can feed on more than 300 kinds of cultivated fruit and vegetable crops and wild plants. The fruit fly has the characteristics of wide host, fast spread, hidden damage and overlapping generations , causing huge harm and economic losses to agricultural production and agricultural trade. At present, the control of B. citrifolia mainly relies on the application of a large number of chemical pesticides. The long-term use of chemical pesticides will lead to major problems such as increased drug resistance of B. citrifolia, increased pesticide residues in fruits and vegetables, and increasingly serious environmental pollution. Biological control is an economical, safe, sustainable and effective method to control the fruit fly. Parasitic wasps are important natural enemy groups of fruit fly pests and occupy an important position in the biological control of pests. Long-tailed Braconid, belonging to Hymenoptera, Braconidae, Braconidae subfamily, Braconidae, also known as Long-tailed Braconid An important parasitic natural enemy of the insects. Liriomyza longiformis can parasitize a variety of fruit flies of economic importance, such as fruit flies of tangerine fruit fly, fruit fly oil, olive fruit fly, etc. Liriomyza longiformis parasitic on fruit fly larvae, by laying eggs in In fruit fly larvae, the eggs live in the host after hatching, destroying the life history of the host and eventually leading to the death of the host. In Latin America and the southern United States, L. long-tailed wasps have been widely used in the biological control of fruit flies, and to solve the problem of indoor propagation of L. long-tailed wasps is the realization of the use of L. long-tailed wasps in the field. key link in biological control. It is of great significance to realize the indoor large-scale breeding of L. long-tailed wasps for the effective use of L. long-tailed wasps for pest biological control.
发明内容SUMMARY OF THE INVENTION
本发明的目的在于提供一种长尾潜蝇茧蜂的室内规模化繁育方法,克服现有技术的不足。The object of the present invention is to provide an indoor large-scale breeding method of Liriomyza longiformis, which overcomes the deficiencies of the prior art.
为实现上述目的,本发明提供了一种长尾潜蝇茧蜂的室内规模化繁育方法,包括以下步骤:In order to achieve the above object, the present invention provides a kind of indoor large-scale breeding method of L. long-tailed wasp, comprising the following steps:
(1)将羽化的长尾潜蝇茧蜂置于养蜂笼中饲养,养蜂笼顶部悬挂有取食装置,所述取食装置内分别装有15%的蜂蜜水,饲养3天后备用;(1) the eclosion was placed in a beekeeping cage to raise, and the top of the beekeeping cage is hung with a feeding device, and 15% honey water is respectively housed in the feeding device, and it is for subsequent use after raising 3 days;
(2)将2~3日龄橘小实蝇幼虫和幼虫饲料放入接种盒内,将接种盒放入步骤(1)中的养蜂笼内进行接种,12~18h后更换新的接种盒,共更换3~4次;(2) Put the 2-3 day old fruit fly larvae and larval feed into the inoculation box, put the inoculation box into the beekeeping cage in step (1) for inoculation, and replace the new inoculation box after 12 to 18 hours , a total of 3 to 4 times of replacement;
(3)将更换出的接种盒内橘小实蝇幼虫和幼虫饲料放入饲养盘内,添加饲料继续饲养,待幼虫发育至7日龄时,将饲养盘移至底部铺有湿沙层的化蛹箱中让幼虫化蛹,化蛹10~14日分筛收集剩下未羽化的蛹,即为寄生蛹;(3) Put the juvenile fruit fly larvae and larval feed in the replaced inoculation box into the feeding tray, add feed and continue to raise, when the larvae develop to 7 days of age, the feeding tray is moved to the bottom covered with wet sand layer. In the pupation box, the larvae are pupated, and the 10-14 days of pupation are screened to collect the remaining unemerged pupae, which are parasitic pupae;
(4)将步骤(3)的寄生蛹放置在底部铺有湿沙的塑料盘中,在所述寄生蛹上铺一层湿沙,将所述塑料盘移置养蜂笼中,至长尾潜蝇茧蜂羽化,即得长尾潜蝇茧蜂。(4) the parasitic pupa of step (3) is placed in the plastic dish that the bottom is covered with wet sand, a deck of wet sand is spread on the described parasitic pupa, the plastic dish is displaced in the beekeeping cage, to the long tail Liriomyia spp. emerges, that is, the long-tailed Liriomys spp.
优选的,上述方法中,所述步骤(1)中,长尾潜蝇茧蜂雄蜂与雌蜂数量比例为1:1~2;养蜂笼内温度为26±1℃,湿度为60~70%,光照周期为14小时光照、10小时黑暗。Preferably, in the above method, in the step (1), the ratio of the number of males and females of Liriomyza longiformis is 1:1-2; the temperature in the beekeeping cage is 26±1°C, and the humidity is 60-70°C %, the light cycle is 14 hours of light and 10 hours of darkness.
优选的,上述方法中,所述步骤(2)中,接种时长尾潜蝇茧蜂与橘小实蝇幼虫数量比为1:20~30;接种盒包括盒体和盒盖,盖体的顶部由50~60目纱网构成,盒体的高度为5~7mm,接种盒内饲料厚度为4~7mm。纱网目数太小导致幼虫逃逸,目数太大时长尾潜蝇茧蜂无法穿刺和寄生。橘小实蝇幼虫具有负趋光性,都位于饲料底部取食,饲料厚度太厚时,幼虫所处位置距离饲料表面越远,长尾潜蝇茧蜂难以在其上产卵寄生。Preferably, in the above method, in the step (2), the ratio of the number of L. long-tailed Brassica and B. tangerines larvae is 1:20 to 30 during inoculation; the inoculation box includes a box body and a box cover, and the top of the cover body is It is composed of 50-60 mesh gauze, the height of the box body is 5-7mm, and the thickness of the feed in the inoculation box is 4-7mm. If the mesh size is too small, the larvae will escape, and if the mesh size is too large, L. long-tailed wasps cannot pierce and parasitize. B. tangerines larvae have negative phototaxis, and they all feed at the bottom of the feed. When the thickness of the feed is too thick, the farther the larvae are from the surface of the feed, it is difficult for L. long-tailed Braconia to lay eggs and parasitize on it.
优选的,上述方法中,所述步骤(3)和步骤(4)中,湿沙为经消毒灭菌的细沙,湿沙的含水量为10~15%。Preferably, in the above method, in the steps (3) and (4), the wet sand is sterilized fine sand, and the moisture content of the wet sand is 10-15%.
优选的,上述方法中,所述取食装置包括桶体,所述取食装置包括桶体,所述桶体顶部设置有桶盖,所述桶盖上设有通孔,所述通孔内设有可拆卸的海绵棒,所述海绵棒一端延伸至桶体底部,另一端从所述通孔向外伸出;吊杆,一端连接所述桶盖,另一端设有吊钩。Preferably, in the above method, the feeding device includes a barrel body, the feeding device includes a barrel body, a barrel cover is provided on the top of the barrel body, and a through hole is provided on the barrel cover, and the through hole is A detachable sponge bar is provided, one end of the sponge bar extends to the bottom of the barrel body, and the other end protrudes out from the through hole; one end of the suspension rod is connected to the barrel cover, and the other end is provided with a hook.
优选的,上述方法中,所述接种盒、饲料盘、化蛹箱和塑料盘一体化为接种化蛹装置,所述接种化蛹装置包括箱体、箱盖、圆形饲料盘、竖直连杆、支架和抽屉式底托,所述箱体顶部开口;Preferably, in the above method, the inoculation box, the feed tray, the pupation box and the plastic tray are integrated into an inoculation and pupation device, and the inoculation and pupation device includes a box body, a box cover, a circular feed tray, a vertical connection poles, brackets and drawer bottom brackets, the top of the box is open;
所述箱盖以可拆卸的方式盖于所述顶部开口,所述箱盖上开设有盖孔,所述盖孔上设有纱网,所述盖孔四周设有一圈向上凸圈,所述凸圈内开设有一圈开口朝下的环形槽;所述箱盖的底面上设有卡座,两个所述卡座均匀分布于所述盖孔的环周,所述卡座朝向所述盖孔的一侧开有卡槽;The box cover is detachably covered on the top opening, a cover hole is opened on the box cover, a gauze is arranged on the cover hole, an upward convex ring is arranged around the cover hole, the An annular groove with an opening facing downward is provided in the convex ring; the bottom surface of the box cover is provided with a catch, two catches are evenly distributed on the circumference of the cover hole, and the catch faces the cover There is a slot on one side of the hole;
所述圆形饲料盘大小与所述环形槽相匹配,外侧面对称设有两个卡块,所述卡块的厚度与所述卡槽相适应,能匹配卡入所述卡槽内;The size of the circular feed pan matches the annular groove, and two clamping blocks are symmetrically arranged on the outer side, the thickness of the clamping blocks is adapted to the clamping groove, and can be matched and inserted into the clamping groove;
所述竖直连杆一端固定在所述圆形饲料盘内底面上,另一端穿过所述纱网向外延伸;One end of the vertical link is fixed on the inner bottom surface of the circular feed pan, and the other end extends outward through the gauze;
所述支架设置在箱体内中部两侧上,用于放置所述圆形饲料盘;The brackets are arranged on both sides of the middle part of the box, and are used for placing the circular feed pan;
所述抽屉式底托设置在箱体内底部,底托内铺设消毒灭菌的湿细沙。The drawer-type bottom support is arranged at the bottom of the box body, and the sterilized wet fine sand is laid in the bottom support.
优选的,上述接种化蛹装置中,所述纱网目数为50~60目。Preferably, in the above-mentioned inoculation and pupation device, the mesh number of the gauze is 50-60 meshes.
与现有的技术相比,本发明具有如下有益效果:Compared with the prior art, the present invention has the following beneficial effects:
1.本发明中的长尾潜蝇茧蜂的室内规模化繁育方法简单易行,可在室内操作,能够大规模繁殖长尾潜蝇茧蜂,实现该茧蜂的终年不间断繁殖,不仅可满足试验研究的需要,还可以实现向瓜果种植区提供大量的虫源,用于防止危害瓜果作物的橘小实蝇。1. The indoor large-scale breeding method of L. long-tailed wasp in the present invention is simple and easy to do, can be operated indoors, can breed L. long-tailed wasp on a large scale, realizes the year-round uninterrupted reproduction of this wasp, not only can To meet the needs of experimental research, it can also provide a large number of insect sources to the melon and fruit planting areas to prevent fruit flies from harming melon and fruit crops.
2.本发明的繁育方法中在接种时控制接种寄生时间,避免了因寄生时间过短导致的低寄生率或无过多选择性而造成低出蜂率,从而提高长尾潜蝇茧蜂的寄生率。2. In the breeding method of the present invention, the inoculation parasitic time is controlled during inoculation, so as to avoid the low parasitic rate caused by the parasitic time being too short or cause a low bee out rate without too much selectivity, thereby improving the L. long-tailed wasp's growth rate. parasitic rate.
3.本发明中的接种化蛹装置将接种、后续饲养和化蛹一体化,减少操作步骤,使操作更加简便,整个过程无需转移幼虫和蛹,可以防止幼虫逃跑和在转移过程对幼虫和蛹造成伤害;提高了长尾潜蝇茧蜂的寄生率和羽化率,加快了长尾潜蝇茧蜂繁育。3. The inoculation and pupation device in the present invention integrates inoculation, subsequent feeding and pupation, reduces the operation steps, and makes the operation more convenient, the whole process does not need to transfer larvae and pupae, and can prevent the larvae from escaping and the transfer process to the larvae and pupae. Inflict damage; increase the parasitism rate and eclosion rate of L. long-tailed wasps, and speed up the breeding of L. long-tailed wasps.
附图说明Description of drawings
图1是本发明实施例2中接种化蛹装置结构图。Fig. 1 is the structure diagram of the device for inoculation and pupation in Example 2 of the present invention.
图2是本发明实施例2中接种化蛹装置剖面图。2 is a sectional view of the inoculation and pupation device in Example 2 of the present invention.
主要附图标记说明:Description of main reference signs:
1-箱体,2-箱盖,3-圆形饲料盘,4-竖直连杆,5-支架,6-抽屉式底托,7-盖孔,8-纱网,9-凸圈,10-卡座,11-卡槽,12-卡块,13-环形槽,14-套环。1- Box body, 2- Box cover, 3- Round feed pan, 4- Vertical link, 5- Bracket, 6- Drawer bottom bracket, 7- Cover hole, 8- Screen mesh, 9- Raised ring, 10-card seat, 11-card slot, 12-card block, 13-ring groove, 14-ring.
具体实施方式Detailed ways
下面结合附图,对本发明的具体实施方式进行详细描述,但应当理解本发明的保护范围并不受具体实施方式的限制。The specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.
实施例1Example 1
一种长尾潜蝇茧蜂的室内规模化繁育方法,包括以下步骤:An indoor large-scale breeding method of Liriomyza longiformis, comprising the following steps:
(1)将羽化的长尾潜蝇茧蜂置于养蜂笼中饲养,养蜂笼内长尾潜蝇茧蜂的密度为1万只/立方米,长尾潜蝇茧蜂雄蜂与雌蜂数量比例为1:1,养蜂笼顶部悬挂有取食装置,取食装置内分别装有15%的蜂蜜水,供长尾潜蝇茧蜂补充营养成分和水分,养蜂笼内温度为26±1℃,湿度为65%,光照周期为14小时光照、10小时黑暗,饲养4天后备用;(1) The eclosion was placed in a bee-keeping cage, and the density of the L. long-tailed wasp in the beekeeping cage was 10,000/m3, and the male and female L. The ratio of quantity is 1:1. There is a feeding device hanging on the top of the beekeeping cage. The feeding device is filled with 15% honey water, which is used to supplement nutrients and water for L. long-tailed cocoon. The temperature in the beekeeping cage is 26%. ±1°C, humidity of 65%, light cycle of 14 hours of light and 10 hours of darkness, and kept for 4 days after rearing;
(2)将3日龄橘小实蝇幼虫和幼虫饲料放入接种盒内,接种盒包括盒体和盒盖,盖体的顶部由60目纱网构成,盒体的高度为6mm,接种盒内饲料厚度为6mm,将接种盒放入步骤(1)中的养蜂笼内进行接种,长尾潜蝇茧蜂通过纱网孔穿刺和寄生,12h后取出接种盒;长尾潜蝇茧蜂羽化后3天为产卵高峰期,每天放入新的接种盒,共更换3次;接种时长尾潜蝇茧蜂与橘小实蝇幼虫数量比为1:30;(2) Put the 3-day-old orange fruit fly larvae and the larval feed into the inoculation box. The inoculation box includes a box body and a box cover. The top of the cover body is composed of 60 mesh gauze. The height of the box body is 6 mm. The thickness of the inner feed is 6 mm, the inoculation box is put into the beekeeping cage in step (1) for inoculation, the L. long-tailed wasp is punctured and parasitized through the gauze mesh, and the inoculation box is taken out after 12 hours; 3 days after eclosion is the peak egg-laying period, and new inoculation boxes are put in every day for a total of 3 replacements; the ratio of the number of larvae of L. long-tailed wasp to B. citricarpa is 1:30 during inoculation;
(3)将取出的接种盒内橘小实蝇幼虫和幼虫饲料放入饲养盘内,根据幼虫数量再添加一定量饲料继续饲养橘小实蝇幼虫,待幼虫发育至7日龄时,将饲养盘移至底部铺有湿沙层的化蛹箱中让幼虫化蛹,化蛹10~14日分筛收集剩下未羽化的蛹,即为寄生蛹;湿沙为经消毒灭菌的细沙,湿沙的含水量为15%;(3) Put the B. tangerineum larvae and larval feed in the taken out inoculation box into the feeding tray, add a certain amount of feed according to the number of larvae and continue to raise B. tangerineum larvae, when the larvae develop to 7 days of age, the feeding The plate is moved to the pupation box that is covered with wet sand layer at the bottom to make the larvae pupate, and the pupae that are left unemerged are collected by sieving on the 10th to 14th day of pupation, which are the parasitic pupae; the wet sand is the fine sand that has been disinfected and sterilized. , the moisture content of wet sand is 15%;
(4)将步骤(3)的寄生蛹放置在底部铺有湿沙的塑料盘中,在寄生蛹上铺一层湿沙,将塑料盘移置养蜂笼中,至长尾潜蝇茧蜂羽化,即得长尾潜蝇茧蜂。(4) the parasitic pupa of step (3) is placed in the plastic dish that the bottom is covered with wet sand, a layer of wet sand is spread on the parasitic pupae, the plastic dish is moved in the beekeeping cage, to the long-tailed Liriomys praecox Emergence, that is, the long-tailed Liriomyza sativae.
取食装置包括桶体、桶盖、海绵棒和吊杆,桶体内部装有15%的蜂蜜水。桶体顶部设置有桶盖,桶盖通过螺纹与桶体可拆卸连接。桶盖上设有一通孔,通孔内设有可拆卸的海绵棒,海绵棒一端延伸至桶体内底部,另一端从通孔向外伸出。吊杆一端固定连接在桶盖上,另一端设有吊钩。取食装置通过吊钩悬挂在养蜂笼顶部,海绵棒将蜂蜜水从桶内吸附到海绵棒外露上端供长尾潜蝇茧蜂吸食,能够有效防止杂质的进入蜂蜜水内及短时间内变质,减少更换频率。The feeding device includes a bucket body, a bucket cover, a sponge stick and a boom, and the inside of the bucket body is filled with 15% honey water. The top of the barrel body is provided with a barrel cover, and the barrel cover is detachably connected to the barrel body through threads. The barrel cover is provided with a through hole, a detachable sponge bar is arranged in the through hole, one end of the sponge bar extends to the bottom of the barrel body, and the other end extends outward from the through hole. One end of the boom is fixedly connected to the barrel cover, and the other end is provided with a hook. The feeding device is hung on the top of the beekeeping cage through the hook, and the sponge stick absorbs the honey water from the bucket to the exposed upper end of the sponge stick for the long-tailed L. long-tailed cocoon to eat, which can effectively prevent impurities from entering the honey water and deteriorate in a short time. , reduce the frequency of replacement.
实施例2Example 2
如图1和图2所示,一种接种化蛹装置,包括箱体1、箱盖2、圆形饲料盘3、竖直连杆4、支架5、抽屉式底托6、盖孔7、纱网8、凸圈9、卡座10、卡槽11、卡块12、环形槽13和套环14。箱体1顶部开口,箱盖2盖于箱体1顶部开口处,箱盖2上开设盖孔7,盖孔7上罩有纱网8,有利于通风透气,纱网为60目,纱网中部固定设置有套环。盖孔7四周设有一圈向上凸圈9,凸圈9内设有一圈开口朝下的环形槽13。箱盖2的底面上固定设置两个卡座10,两个卡座10均匀分布于盖孔7的环周,卡座10朝向盖孔7的一侧开有卡槽11。圆形饲料盘3大小与环形槽13相匹配,圆形饲料盘3侧壁能够卡入环形槽13内。两个卡块12固定设置在圆形饲料盘3外侧面的中部,位置对称,卡块12的厚度与卡槽11相适应,能匹配卡入卡槽11内。竖直连杆4一端固定在圆形饲料盘3内底面中部,另一端穿过套环14向外延伸,竖直连杆4可以在套环14自由滑动。支架5固定设置在箱体内两相对侧面,用于放置圆形饲料盘3。抽屉式底托6设置在箱体1内底部,箱体1前侧下部开设开口,用于抽屉式底托6放入和抽出。抽屉式底托6内铺设消毒灭菌的湿沙,湿沙水分含量为15%。As shown in Figures 1 and 2, an inoculation and pupation device includes a
接种时,取下箱盖2,将橘小实蝇幼虫和幼虫饲料放入圆形饲料盘3中,盖上箱盖2,向上拉动竖直连杆4带动圆形饲料盘3向上,圆形饲料盘3上部分卡入环形槽13内,转动竖直连杆4,卡块12卡入卡槽11内,圆形饲料盘3得以固定,圆形饲料盘3下部分和纱网构成接种盒进行接种,橘小实蝇幼虫和幼虫饲料被限制在圆形饲料盘3下部分,防止幼虫弹跳,便于长尾潜蝇茧蜂进行寄生;接种完成后,转动竖直连杆4将卡块12从卡槽11内转出,向下推竖直连杆4,直到圆形饲料盘3置于支架5上,打开箱盖再加入一定量的饲料继续饲养幼虫。幼虫发育成老熟幼虫,老熟幼虫弹跳入抽屉式底托的湿沙中化蛹,化蛹8~10日非寄生蛹羽化爬出湿沙,未羽化的蛹即为寄生蛹,将抽屉式托底从箱体底部抽出,放入养蜂笼中,至长尾潜蝇茧蜂羽化。When inoculating, remove the
接种化蛹装置为接种、化蛹等提供一个良好的环境,将接种、后续饲养和化蛹一体化,减少操作步骤,使操作更加简便,整个过程无需转移幼虫和蛹,可以防止幼虫逃跑和在转移过程对幼虫和蛹造成伤害;寄生蛹的蛹期是长尾潜蝇茧蜂各种器官和系统形成期,极易受到机械损伤,接种化蛹装置避免了筛分寄生蛹过程有效保护了寄生蛹,提高了长尾潜蝇茧蜂的寄生率和羽化率,加快了长尾潜蝇茧蜂繁殖效率。The inoculation and pupation device provides a good environment for inoculation, pupation, etc. It integrates inoculation, subsequent feeding and pupation, reduces the operation steps, and makes the operation easier. The whole process does not need to transfer larvae and pupae, which can prevent the larvae from escaping and in The transfer process causes damage to the larvae and pupae; the pupal stage of the parasitic pupae is the period of the formation of various organs and systems of L. long-tailed wasps, which are extremely susceptible to mechanical damage. The inoculation pupation device avoids the process of screening the parasitic pupae and effectively protects the parasitic The pupa can improve the parasitism rate and eclosion rate of L. long-tailed wasp, and speed up the reproduction efficiency of L. long-tailed wasp.
本实施例提供一种长尾潜蝇茧蜂的室内规模化繁育方法,包括以下步骤:The present embodiment provides an indoor large-scale breeding method of L. long-tailed Braconite, comprising the following steps:
(1)将羽化的长尾潜蝇茧蜂置于养蜂笼中饲养,养蜂笼内长尾潜蝇茧蜂的密度为1万只/立方米,长尾潜蝇茧蜂雌蜂与雄蜂数量比例为1:1,养蜂笼顶部悬挂有取食装置,取食装置内分别装有15%的蜂蜜水,供长尾潜蝇茧蜂补充营养成分和水分,养蜂笼内温度为26±1℃,湿度为65%,光照周期为14小时光照、10小时黑暗,饲养4天后备用;(1) The emerging L. long-tailed wasp was raised in a beekeeping cage, and the density of the long-tailed L. long-tailed wasp in the beekeeping cage was 10,000/m3, and the female and male L. long-tailed wasp were The ratio of quantity is 1:1. There is a feeding device hanging on the top of the beekeeping cage. The feeding device is filled with 15% honey water, which is used to supplement nutrients and water for L. long-tailed cocoon. The temperature in the beekeeping cage is 26%. ±1°C, humidity of 65%, light cycle of 14 hours of light and 10 hours of darkness, and kept for 4 days after rearing;
(2)将3日龄橘小实蝇幼虫和幼虫饲料放入接种化蛹装置的圆形饲料盘中,饲料厚度5mm,拉动竖直连杆将圆形饲料盘上部分卡入环形槽内,圆形饲料盘下部分和纱网构成接种盒,圆形饲料盘下部分的高度为6mm,将接种化蛹装置放入步骤(1)中的养蜂笼内进行接种,12h后取出接种化蛹装置,羽化后3天为长尾潜蝇茧蜂产卵高峰期,每天放入新的接种化蛹装置,共更换3次;接种时长尾潜蝇茧蜂与橘小实蝇幼虫的数量比为1:30;(2) put 3-day-old orange fruit fly larvae and larval feed into the circular feed tray of the inoculation pupation device, the feed thickness is 5mm, and the upper part of the circular feed tray is stuck in the annular groove by pulling the vertical connecting rod, The lower part of the circular feed tray and the gauze form an inoculation box, the height of the lower part of the circular feed tray is 6mm, the inoculation pupation device is put into the beekeeping cage in step (1) for inoculation, and the inoculation pupation is taken out after 12h The device, 3 days after eclosion is the peak egg-laying period of L. long-tailed wasp, and a new inoculation and pupation device was put in every day, and replaced 3 times; 1:30;
(3)将取出的接种化蛹装置内的圆形饲料盘推至支架上,打开箱盖加入适量的幼虫饲料,在接种化蛹装置内继续饲养幼虫,幼虫发育至7日龄时为老熟幼虫,老熟幼虫弹跳入抽屉式托底湿沙内化蛹,化蛹10~14日后未被寄生的蛹开始羽化成橘小实蝇成虫,爬出湿沙,清理接种化蛹装置内橘小实蝇成虫,抽屉式托底湿沙内未羽化的蛹即为寄生蛹,取出抽屉式托底;(3) push the circular feed plate in the inoculated pupation device taken out to the support, open the box cover and add an appropriate amount of larvae feed, continue to raise larvae in the inoculated pupation device, and the larvae are mature when they develop to 7 days of age Larvae, mature larvae bounce into the wet sand of the drawer-type support bottom to pupate, 10 to 14 days after pupation, the unparasitic pupae begin to emerge into adults of B. tangerines, climb out of the wet sand, and clean the inoculated pupation device. Fruit fly adults, the pupa that does not emerge in the wet sand of the drawer-type support bottom is the parasitic pupa, and the drawer-type support bottom is taken out;
(4)将步骤(3)的抽屉式托底移置养蜂笼中,至长尾潜蝇茧蜂羽化,即得长尾潜蝇茧蜂。(4) The drawer-type support bottom of step (3) is transferred into the beekeeping cage, until the L. long-tailed wasp emerges, that is, the long-tailed L. long-tailed wasp is obtained.
实施例3Example 3
一种长尾潜蝇茧蜂的室内规模化繁育方法,包括以下步骤:An indoor large-scale breeding method of Liriomyza longiformis, comprising the following steps:
(1)将羽化的长尾潜蝇茧蜂置于养蜂笼中饲养,养蜂笼内长尾潜蝇茧蜂的密度为1万只/立方米,长尾潜蝇茧蜂雄蜂与雌蜂数量比例为1:2,养蜂笼顶部悬挂有取食装置,取食装置内分别装有15%的蜂蜜水,供长尾潜蝇茧蜂补充营养成分和水分,养蜂笼内温度为26±1℃,湿度为70%,光照周期为14小时光照、10小时黑暗,饲养4天后备用;(1) The emerging L. long-tailed wasp was raised in a beekeeping cage, and the density of the long-tailed L. long-tailed wasp in the beekeeping cage was 10,000/m3, and the male and female L. long-tailed wasp were The ratio of quantity is 1:2. There is a feeding device hanging on the top of the beekeeping cage. The feeding device is filled with 15% honey water, which is used to supplement nutrients and water for L. long-tailed cocoon. The temperature in the beekeeping cage is 26%. ±1°C, humidity of 70%, light cycle of 14 hours of light and 10 hours of darkness, and kept for 4 days after rearing;
(2)将2日龄橘小实蝇幼虫和幼虫饲料放入接种盒内,接种盒包括盒体和盒盖,盖体的顶部由60目纱网构成,盒体的高度为6mm,接种盒内饲料厚度为6mm,将接种盒放入步骤(1)中的养蜂笼内进行接种,接种时长尾潜蝇茧蜂与橘小实蝇幼虫的数量比为1:20,长尾潜蝇茧蜂通过纱网孔穿刺和寄生,18h后取出接种盒;放入新的接种盒,羽化后3天为长尾潜蝇茧蜂产卵高峰期,可每天放入新的接种盒,共更换3次;(2) Put the 2-day-old tangerine fruit fly larvae and the larval feed into the inoculation box. The inoculation box includes a box body and a box cover. The top of the cover body is composed of 60 mesh gauze. The height of the box body is 6 mm. The thickness of the inner feed is 6 mm, and the inoculation box is put into the beekeeping cage in step (1) for inoculation. During the inoculation, the ratio of the quantity of L. long-tailed cocoon to the larvae of B. citricarpa is 1:20, and the cocoon of L. long-tailed cocoon is 1:20. The bees puncture and parasitize through the mesh holes, and take out the inoculation box after 18 hours; put them in a new inoculation box, and 3 days after eclosion is the peak egg laying period of L. long-tailed wasps, and a new inoculation box can be placed every day for a total of 3 replacements. Second-rate;
(3)将取出的接种盒内橘小实蝇幼虫和幼虫饲料放入饲养盘内,根据幼虫数量再添加一定量饲料继续饲养橘小实蝇幼虫,待幼虫发育至7日龄时,将饲养盘移至底部铺有湿沙层的化蛹箱中让幼虫化蛹,化蛹10~14日后分筛收集剩下未羽化的蛹,即为寄生蛹;湿沙为经消毒灭菌的细沙,湿沙的含水量为15%;(3) Put the B. tangerineum larvae and larval feed in the taken out inoculation box into the feeding tray, add a certain amount of feed according to the number of larvae and continue to raise B. tangerineum larvae, when the larvae develop to 7 days of age, the feeding The plate is moved to the pupation box that is covered with wet sand layer at the bottom to make the larvae pupate, and after 10 to 14 days of pupation, the remaining unemerged pupae are collected by sieving, which is the parasitic pupae; the wet sand is the fine sand that has been disinfected and sterilized. , the moisture content of wet sand is 15%;
(4)将步骤(3)的寄生蛹放置在底部铺有湿沙的塑料盘中,在寄生蛹上铺一层湿沙,将塑料盘移置养蜂笼中,至长尾潜蝇茧蜂羽化,即得长尾潜蝇茧蜂。(4) the parasitic pupa of step (3) is placed in the plastic dish that the bottom is covered with wet sand, a layer of wet sand is spread on the parasitic pupae, the plastic dish is moved in the beekeeping cage, to the long-tailed Liriomys praecox Emergence, that is, the long-tailed Liriomyza sativae.
室内繁育试验indoor breeding test
1、材料1. Materials
供试虫源:寄主虫源为幼虫为经室内大量饲养的橘小实蝇幼虫;长尾潜蝇茧蜂,在室内繁殖建立稳定的实验种群。The source of the tested insects: the host insect source is the larvae of B. citricarpa, which are reared in large quantities indoors; the L. long-tailed Braconid, which breeds indoors to establish a stable experimental population.
试验方法:分别设置实施例1组、实施例2组、实施例3组、对比1组和对比2组,实施例1组、实施例2组和实施例3组分别采用实施例1、实施例2和实施例3提供的方法进行长尾潜蝇茧蜂繁育;对比1组采用1日龄的橘小实蝇幼虫作为寄主虫源,其他参数和方法步骤与实施例1相同;对比2组接种时间为24h,其他参数和方法步骤与实施例1相同。观察各组长尾潜蝇茧蜂的寄生率和羽化率。Test method: Example 1 group, Example 2 group, Example 3 group,
表1长尾潜蝇茧蜂的寄生率和羽化率Table 1 Parasitism rate and emergence rate of L. long-tailed Braconite
从表1可以看出,1日龄的幼虫可以寄生,但是寄生率明显低于2~3日龄幼虫的寄生率,羽化率也明显偏低。寄生时间为24h的寄长尾潜蝇茧蜂羽化率明显较低,寄生时间过长时会产生过寄生现象,降低长尾潜蝇茧蜂的羽化率。结果表明,本发明提供长尾潜蝇茧蜂的室内规模化繁育方法,有效提高了长尾潜蝇茧蜂的寄生率和羽化率,能够进行长尾潜蝇茧蜂大规模繁育。It can be seen from Table 1 that the 1-day-old larvae can be parasitized, but the parasitic rate is significantly lower than that of the 2-3-day-old larvae, and the emergence rate is also significantly lower. The 24h parasitic time of L. long-tailed wasps had a significantly lower emergence rate. If the parasitization time was too long, over-parasitic phenomenon would occur, which reduced the emergence rate of L. long-tailed wasps. The results show that the present invention provides an indoor large-scale breeding method of L. long-tailed wasp, effectively improves the parasitic rate and eclosion rate of L. long-tailed wasp, and enables large-scale breeding of L. long-tailed wasp.
前述对本发明的具体示例性实施方案的描述是为了说明和例证的目的。这些描述并非想将本发明限定为所公开的精确形式,并且很显然,根据上述教导,可以进行很多改变和变化。对示例性实施例进行选择和描述的目的在于解释本发明的特定原理及其实际应用,从而使得本领域的技术人员能够实现并利用本发明的各种不同的示例性实施方案以及各种不同的选择和改变。本发明的范围意在由权利要求书及其等同形式所限定。The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. These descriptions are not intended to limit the invention to the precise form disclosed, and obviously many changes and modifications are possible in light of the above teachings. The exemplary embodiments were chosen and described for the purpose of explaining certain principles of the invention and their practical applications, to thereby enable one skilled in the art to make and utilize various exemplary embodiments and various different aspects of the invention. Choose and change. The scope of the invention is intended to be defined by the claims and their equivalents.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112602629A (en) * | 2020-12-10 | 2021-04-06 | 重庆市畜牧科学院 | Device and method for breeding drone colony |
CN112868607A (en) * | 2021-03-01 | 2021-06-01 | 闫丰军 | Method for controlling bad pupa disease of Chouioia cunea Yang breeding |
CN113892465A (en) * | 2021-10-08 | 2022-01-07 | 福建艳璇生物防治技术有限公司 | Method for automatically propagating fruit fly parasitic wasps in field |
CN114081016A (en) * | 2021-11-24 | 2022-02-25 | 宁夏农林科学院植物保护研究所(宁夏植物病虫害防治重点实验室) | System for detecting emergence rate of myzus persicae and using method thereof |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140261188A1 (en) * | 2012-04-03 | 2014-09-18 | Byeong-Gyu Chin | Apparatus for breeding fly larvae |
CN104872068A (en) * | 2015-05-26 | 2015-09-02 | 中国热带农业科学院环境与植物保护研究所 | Indoor insectary and culture method for thrips hawaiiensis |
CN105165800A (en) * | 2015-10-21 | 2015-12-23 | 广东省粮食科学研究所 | Parasitic wasp natural enemy release device and parasitic wasp natural enemy sustained release method |
CN107517934A (en) * | 2017-09-06 | 2017-12-29 | 云南省林业科学院 | A kind of more worm state box for feeding pests of citrus fruit fly and its method for breeding |
CN110679553A (en) * | 2019-11-22 | 2020-01-14 | 中国热带农业科学院环境与植物保护研究所 | A large-scale propagation method for the parasitic wasp of the cucurbit fly larvae |
-
2020
- 2020-03-25 CN CN202010219324.XA patent/CN111328770B/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20140261188A1 (en) * | 2012-04-03 | 2014-09-18 | Byeong-Gyu Chin | Apparatus for breeding fly larvae |
CN104872068A (en) * | 2015-05-26 | 2015-09-02 | 中国热带农业科学院环境与植物保护研究所 | Indoor insectary and culture method for thrips hawaiiensis |
CN105165800A (en) * | 2015-10-21 | 2015-12-23 | 广东省粮食科学研究所 | Parasitic wasp natural enemy release device and parasitic wasp natural enemy sustained release method |
CN107517934A (en) * | 2017-09-06 | 2017-12-29 | 云南省林业科学院 | A kind of more worm state box for feeding pests of citrus fruit fly and its method for breeding |
CN110679553A (en) * | 2019-11-22 | 2020-01-14 | 中国热带农业科学院环境与植物保护研究所 | A large-scale propagation method for the parasitic wasp of the cucurbit fly larvae |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112602629A (en) * | 2020-12-10 | 2021-04-06 | 重庆市畜牧科学院 | Device and method for breeding drone colony |
CN112602629B (en) * | 2020-12-10 | 2022-09-27 | 重庆市畜牧科学院 | A kind of device and method for cultivating colony of drones |
CN112868607A (en) * | 2021-03-01 | 2021-06-01 | 闫丰军 | Method for controlling bad pupa disease of Chouioia cunea Yang breeding |
CN112868607B (en) * | 2021-03-01 | 2022-12-16 | 闫丰军 | Control method of breeding bad pupae disease of white moth Zhou's gnats |
CN113892465A (en) * | 2021-10-08 | 2022-01-07 | 福建艳璇生物防治技术有限公司 | Method for automatically propagating fruit fly parasitic wasps in field |
CN114081016A (en) * | 2021-11-24 | 2022-02-25 | 宁夏农林科学院植物保护研究所(宁夏植物病虫害防治重点实验室) | System for detecting emergence rate of myzus persicae and using method thereof |
CN114081016B (en) * | 2021-11-24 | 2023-08-11 | 宁夏农林科学院植物保护研究所(宁夏植物病虫害防治重点实验室) | Detection system for emergence rate of aphidius gifuensis and application method thereof |
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