CN111307678A - A kind of cell suspension concentration measurement and liquid dispensing device and working method - Google Patents

Abstract

The invention discloses a cell suspension concentration measurement and liquid dispensing device and a working method, which are characterized by comprising an MCU, a monochromatic light source, a cuvette, a photoelectric detector, a cassette, a light guide tube, a quantitative liquid taking device, a Liquid rod; the device uses a monochromatic LED as the light source, without the need for a collimating lens to collimate the light source, a light guide tube with a certain length and diameter is directly used to realize the function of the collimating lens; it also does not need a filter to filter out other wavelength of light; as long as a certain amount of cell suspension is sampled and added to the cuvette for measurement before the liquid-based thin-layer cytology tablet machine is used, the concentration of the cell suspension to be tested can be well estimated. , according to the given dilution ratio parameters, the dilution ratio is carried out to obtain the diluted cell suspension. The samples prepared under the dilution ratio concentration have good uniformity, which can greatly improve the quality of the tablet and overcome the It solves the problems of large differences and poor consistency in the results of empty films, stripping, stacking and filming.

Description

A kind of cell suspension concentration measurement and liquid dispensing device and working method

technical field

The invention belongs to the field of biomedical instrument design, and in particular relates to a cell suspension concentration measurement and liquid dispensing device and a working method for improving the quality of liquid-based thin-layer cytology tableting.

Background technique

Cervical cancer is the second most common malignant tumor in women, and its incidence ranks first among malignant tumors of the female genital tract. In 2015, the number of new cases in China was 98,900, the number of deaths was 30,500, and the incidence of cervical cancer showed a younger trend. The traditional manual production method for cervical cancer screening is mainly cervical smear Pap smear, which has a low detection rate of abnormal cervical cells, high false negative, and slow production speed, which cannot meet the large-scale and fast production in modern clinical practice. The need for accurate diagnosis.

The produced liquid-based thin-layer cytology (TCT) preparation methods mainly include centrifugal preparation, membrane preparation and sedimentation preparation. Different preparation methods require different types of equipment. Among them, there is the TCT film tablet machine of hologic company. Due to the presence of impurities in the sample, the membrane will be blocked, so the prepared samples have problems such as large variability and poor uniformity. In addition, there is a sedimentation tablet machine of BD Company, which can automatically complete the preparation of slices and cytological staining, with a clear background and intact cell morphology. However, the pretreatment process is very complicated, the production time is long, and the cells are naturally deposited and stacked, and the cells are not laid on one layer, which will cause inconvenience to the observation under the microscope.

Compared with the traditional production method, TCT production solves the shortcomings of traditional production to a certain extent and improves the production effect. However, because the cell concentration of the cell suspension sample was not monitored during the production process, the prepared cell samples had problems such as dissection, stacking, large differences in the production results of different samples, and poor uniformity. These factors will directly affect the production process. Affecting the doctor's manual microscopic examination or causing errors in machine interpretation after scanning with a pathological scanner, that is, misjudgment. This will reduce the detection rate and require re-production and interpretation, and even the possibility of sub-sampling, thereby increasing the inspection cycle and the possibility of missed and misjudged judgments.

The method generally used for cell counting is the counter assay method, so that the cell concentration can be calculated. This method requires the use of a cell counter and observation and counting with a microscope. The operation is complicated, time-consuming, and cannot be quickly detected. The spectrophotometer that can be used to measure concentration has complex instrument mechanism, high cost and large volume. It is often used for nucleic acid quantification, colorimetric protein quantification, and needs to be equipped with a computer, which is inconvenient to operate and cannot be used for a long time. Continuous work cannot be used in actual measurement in this field. Another method uses a laser as a light source for measurement, and the measurement object is mainly suspended particles, which cannot be used for measurement of transparent cells. The name of the invention is a cell measurement device (authorized announcement number CN 103543093B), and the method involves flow cytometry, so cells need to be fluorescently labeled, and the cells that need to be measured in the art are colorless, transparent, and unlabeled cell suspensions, so it is Cannot be used for measurement. There is also a photoelectric colorimeter to measure the concentration. Its principle is colorimetry, which is based on the color of the solution of the substance to be measured or the colored solution generated after adding a color developer. It cannot be used for colorless and transparent cell concentration. Measurement.

Due to the need to avoid contamination of the sample in actual use, it is not possible to use a device that directly extends into the sample solution to measure its cell concentration, and the total amount of the sample solution is only about 6ml, which cannot be used for devices that require a large amount of cell suspension samples for measurement. in this field.

Therefore, there is a need for an easy-to-use, highly reliable, fast and accurate device for measuring the concentration of cell suspensions, to solve the uniformity of prepared specimens, and to avoid the problems of cell stacking, falling off and empty slices, which can greatly improve the interpretation accuracy. Accuracy and reading efficiency.

SUMMARY OF THE INVENTION

In view of at least one of the above defects or improvement needs of the prior art, the present invention provides a cell suspension concentration measurement and liquid dispensing device and working method, which are used to improve the quality of liquid-based thin-layer cytology tablets. The device uses a monochromatic LED as the light source, and does not need a collimating lens to collimate the light source, and directly uses a light guide tube with a certain length and diameter to realize the function of the collimating lens; it also does not need a filter to filter out other wavelengths of light. ; As long as a certain amount of sample is taken from the cell suspension and added to the cuvette for measurement before the liquid-based thin-layer cytology tableting machine is used, the concentration value of the sample to be tested can be obtained.

In order to achieve the above object, according to one aspect of the present invention, a cell suspension concentration measurement and liquid dispensing device is provided, including MCU, a cell suspension concentration measurement device, and a cell suspension liquid dispensing device;

The cell suspension concentration measurement device includes a monochromatic light source, a cuvette, a photodetector, a cassette, and a light guide; the monochromatic light source, the cuvette, the photodetector, and the light guide are arranged in the cassette. , the cuvette is arranged between the monochromatic light source and the photodetector, and the opposite light guide tubes are arranged on both sides of the cuvette, which are respectively arranged between the monochromatic light source and the monochromatic light source. between and between the photodetector;

The cell suspension liquid dispensing device includes a quantitative liquid taking device and a liquid taking stick; the quantitative liquid taking device is connected to the liquid taking stick, and the liquid taking stick includes a liquid taking stick for the liquid to be measured and a dilution liquid taking stick;

The MCU controls and connects the monochromatic light source, the photodetector, the light guide tube and the quantitative liquid taking device respectively.

Preferably, the cell suspension concentration measurement device further includes a light source driving device, which is connected between the monochromatic light source and the MCU, and is used for maintaining the stability of the light source's luminous intensity and the regulation of the light intensity.

Preferably, the cell suspension concentration measurement device further includes a signal amplification device, connected between the photodetector and the MCU, for collecting the weak current signal generated by the photodetector and converting it into a signal that can be directly collected voltage signal.

Preferably, the cell suspension concentration measuring device further comprises a cassette cover, which is installed in the cassette; when the cassette cover is opened, the cuvette can be taken and placed from the cassette.

Preferably, the quantitative liquid taking device of the cell suspension liquid dispensing device is a microliter grade flow pipetting pump.

Preferably, the microliter grade flow pipetting pump is respectively connected with the liquid sampling stick for the liquid to be tested and the diluting liquid sampling stick through a silicone tube.

Preferably, the cell suspension concentration measurement and liquid dispensing device further includes a display screen, which is connected to the MCU and is used for operation prompts and information display during the measurement and liquid dispensing process, and provides a user interaction interface for the convenience of the operator. .

In order to achieve the above object, according to another aspect of the present invention, there is also provided a working method of the aforementioned cell suspension concentration measurement and liquid dispensing device, comprising the following steps:

S1. Measure the absorption spectrum curve of the cell suspension to obtain the maximum absorption wavelength. Using the monochromatic light source of this wavelength, inject multiple groups of cell suspension samples with different concentrations into the cuvette, and measure the light transmission colorimetry of the monochromatic light source in turn. The transmitted light intensity after the dish and the internal cell suspension sample is converted into absorbance, so as to obtain the standard curve of the relationship between concentration and absorbance;

S2. Put the empty cuvette into the cassette, and the MCU calibrates zero before measuring;

S3. Absorb the fully mixed suspension of cells to be tested, inject it into an empty cuvette, measure the transmitted light intensity of the suspension of cells to be tested, convert it into absorbance, and substitute it into the standard curve of the relationship between concentration and absorbance , obtain the concentration of the cell suspension to be tested;

S4, according to the measured concentration of the cell suspension to be tested and the preset dilution ratio parameters, calculate and control the respective sampling amounts of the liquid to be tested and the diluent, after mixing and fully shaking, That is, the diluted and mixed liquid for tableting is obtained, which is then used for tableting by the tableting machine;

S5. Replace the cuvette, repeat steps S2-S4, and perform the next concentration measurement and solution preparation of the cell suspension to be tested.

Preferably, in step S3, a burette is used to suck the fully mixed suspension of cells to be tested, and the nozzle of the burette is pressed against the inner wall of the empty cuvette to slowly extrude the suspension of cells to be tested in the burette to avoid the generation of air bubbles.

Preferably, in step S4, the liquid sampling rod of the liquid to be tested is connected with a disposable pipette tip, and placed into the cell suspension to be tested that has been fully mixed in step S3, and the MCU controls the sampling amount calculated after taking Then stop sampling, put the pipette tip into the empty test tube, remove the pipette tip after draining it clean; use the diluent pick-up stick to absorb the diluent that calculates the sampling amount, and put the diluent into the same test tube, fully Shake the tube well.

The above preferred technical features can be combined with each other as long as they do not conflict with each other.

In general, compared with the prior art, the above technical solutions conceived by the present invention have the following beneficial effects:

1. The cell suspension concentration measurement and liquid dispensing device and working method of the present invention improve the quality of liquid-based thin-layer cytology preparations; the device uses a monochromatic LED as a light source, and does not require a collimating lens to collimate the light source, A light guide tube with a certain length and diameter is directly used to realize the function of collimating lens; no filter is needed to filter out light of other wavelengths; Take a certain amount of sample and add it to the cuvette for measurement, and then the concentration value of the sample to be measured can be obtained.

2. The cell suspension concentration measurement and dispensing device and working method of the present invention can well estimate the concentration value of the cell suspension to be tested, and perform the dilution ratio according to the given dilution ratio parameters to obtain the diluted cell suspension. The samples prepared at this dilution ratio concentration have good uniformity, which can greatly improve the quality of the tablet, and overcome the large differences in the results of empty tablets, stripping, stacking and tableting, and poor consistency. The problem.

3. The cell suspension concentration measurement and liquid dispensing device and working method of the present invention are finally applied to the filming process of the liquid-based thin-layer cytology tableting machine, which improves the tableting quality, thereby improving the efficiency of manual microscopic examination by doctors. The reading efficiency and the interpretation accuracy of machine reading, especially the detection rate of cervical cancer cells.

4. The cell suspension concentration measurement and liquid dispensing device and working method of the present invention, if the cell suspension to be measured is the same type of cells, it only needs to be calibrated once in the initial use, and then it can be used directly.

Description of drawings

1 is a schematic diagram of the overall structure of a cell suspension concentration measurement and liquid dispensing device according to an embodiment of the present invention;

Fig. 2 is the tablet quality schematic diagram of the A liquid that does not use the concentration measurement of the present invention and the liquid dosing device;

Fig. 3 is the comparison schematic diagram of the tableting quality of the A liquid using the concentration measurement of the present invention and the liquid dosing device;

Fig. 4 is the schematic diagram of the tableting quality of the B liquid that does not use the concentration measurement of the present invention and the liquid dosing device;

Fig. 5 is the comparison schematic diagram of the tableting quality of the B liquid using the concentration measurement of the present invention and the liquid dosing device;

Fig. 6 is the tablet quality schematic diagram of the C liquid that does not use the concentration measurement of the present invention and the liquid dosing device;

7 is a schematic diagram showing the comparison of tableting quality of liquid C using the concentration measurement and liquid dosing device of the present invention.

Detailed ways

In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention, but not to limit the present invention. In addition, the technical features involved in the various embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other. The present invention will be further described in detail below in conjunction with specific embodiments.

As a preferred embodiment of the present invention, as shown in FIG. 1 , the present invention provides a fast and stable cell suspension concentration measurement and liquid dispensing device, including an MCU1, a light source driving device 2, and a monochromatic light source 3 (for example, a monochromatic light source 3). LED light source), cuvette 4, photodetector 5 (such as photodiode), signal amplification device 6, cassette 7, cassette lid 8, light guide tube 9, display screen 10, quantitative liquid taking device 11, silicone tube 12 , Liquid sampling stick 13 , button control area 14 .

MCU1 is used to maintain the normal and stable operation of the entire system and data acquisition, and is powered by a 220V power supply. The light source driving device 2 is connected with the MCU1 through wires, and is used to ensure the constant luminous intensity of the LED light source and the adjustment of the luminous intensity. The LED light source is used to provide a signal source for measurement, is fixed at the middle position on the left side of the cassette 7, and is connected to the light source driving device 2 through wires. The cuvette 4 is used to hold the cell suspension sample to be tested, and is placed in the groove in the middle of the cassette 7, so that the cuvette 4 is located in the middle position between the LED light source and the photodiode. The photodetector 5 is used to detect the transmitted light intensity after the light emitted by the receiving light source passes through the cuvette and the suspension sample to be measured inside. The signal amplifying device 6 is connected with the photodetector through a signal line, and is used to collect the weak current signal generated by the photodetector, convert it into a voltage signal that can be directly collected, and amplify it; the MCU1 communicates with the signal amplifying device 6 through the signal line. It is connected to collect the output voltage signal. The cassette 7 is used to install a fixed light source, a cuvette and a photodetector to constitute the entire reaction generating device. The cassette lid 8 is installed above the cassette, in order to ensure that the entire reaction process is completed under dark conditions. The light guide tube 9 is located on both sides of the cuvette 4, so as to isolate the stray light to a certain extent and play the function of collimation. The display screen 10 is used for the operation prompts during the operation of the operator and the display of the final solution concentration and dilution ratio parameters, and provides a user interaction interface, which is convenient for the operator to use. The quantitative liquid taking device 11 is used to quantitatively take the liquid to be measured and the diluent. The liquid taking stick 13 is connected to the quantitative liquid taking device 11 through the rubber tube 12 , and the liquid taking stick 13 includes a liquid taking stick for the liquid to be measured and a diluting liquid taking stick. The button control area 14 is composed of a measurement button B0, a sample liquid taking and discharging button B1, and a dilution liquid taking and discharging button B2.

The invention uses the LED light source, which has the advantages of low cost, long life, simple structure and easy control and use. In order to improve the accuracy of the measurement system, a light guide tube structure is designed in the cassette 7, and its function is equivalent to collimating the light emitted by the LED light source, ensuring that the parallel light is incident on the solution in the cuvette 4, reducing the The influence of stray light on the measurement. In addition, the beneficial effect of the cuvette 4 is that the transmittance of near-ultraviolet and visible light is higher, the intensity of transmitted light is increased, and the loss of energy is reduced, thereby increasing the concentration range of the measurable cell suspension and improving the measurement accuracy . The used quantitative liquid taking device 11 , such as a peristaltic pump, is a high-precision micro-flow pipetting pump, the precision can reach the microliter level, and the liquid can be accurately and quantitatively pipetted.

The working method of the cell suspension concentration measurement and liquid dispensing device of the present invention comprises the following steps:

S1. Measure the absorption spectrum curve of the cell suspension by a spectrophotometer or a spectrometer to obtain the maximum absorption wavelength, use a monochromatic light source of this wavelength, and perform a calibration (see step S2 below), and then analyze multiple groups of cell suspensions with different concentrations. The sample is injected into the cuvette, and the transmitted light intensity of the light emitted by the monochromatic light source after passing through the cuvette and the internal cell suspension sample is measured in turn, and then converted into absorbance to obtain the standard curve of the relationship between concentration and absorbance;

S2. Turn on the working power of the whole system, after preheating for more than 5 minutes, open the cover 8 of the cassette, put the empty cuvette 4 into the cassette 7, close the cover 8 of the cassette, and press the button according to the prompt on the display screen 10. Measure button B0, MCU zero-calibrates before measuring;

S3. Use a burette to suck up 1.5ml of the cell suspension to be tested that is fully mixed, and press the burette nozzle against the inner wall of the empty cuvette to slowly squeeze out the cell suspension to be tested in the burette to avoid the generation of air bubbles and affect the accuracy of the measurement results. , after adding the cell suspension, cover the cassette lid 8, press the measurement button B0, measure the transmitted light intensity of the cell suspension to be measured, then convert it into absorbance, and substitute it into the standard curve of the relationship between concentration and absorbance , to obtain the concentration of the cell suspension to be tested;

S4, according to the measured concentration of the cell suspension to be tested and the preset dilution ratio parameters, calculate and control the respective sampling amounts of the liquid to be tested and the diluent, mixed and fully shaken, mixed The steps are as follows: connect the liquid sampling stick for the liquid to be tested with the disposable pipette tip, put it into the cell suspension to be tested that has been fully mixed in step S3, press the sample liquid sampling and dispensing button B1, the MCU After controlling the calculated sampling volume, stop sampling when the display indicates the end of liquid sampling, put the pipette tip into the empty test tube, press the sample liquid sampling and dispensing button B1 again, and remove the pipette tip after draining cleanly; the same Ground, through the diluent taking and discharging button B2, use the diluent taking stick to draw the diluent that calculates the sampling amount, and also put the diluent into the same test tube, and fully shake the test tube; that is, to obtain the tablet for tableting. After diluting and mixing 4ml of liquid, remove 2ml of this liquid for tableting machine tablets;

S5. Replace the cuvette, repeat steps S2-S4, and perform the next concentration measurement and solution preparation of the cell suspension to be tested.

It can be seen from Figures 2-7 that the cells in liquid A without the concentration measurement and dosing device of the present invention have cell dissociation, accumulation and unevenness, the cell density in liquid B is high, and liquid C has uneven pictures. The consistency of the single sample glass slide prepared by the concentration measurement and liquid dispensing device of the present invention is not good, and the results of the sample liquid preparations with different concentrations have large differences and poor consistency. However, after using the concentration measurement and liquid dispensing device of the present invention, the cell smear of a single glass slide sample is relatively uniform, and the difference between the results of the sample liquid preparation of different concentrations is small.

After many experimental tests, the cell suspension concentration measurement and liquid dispensing device can well estimate the concentration value of the cell suspension to be tested, and the dilution ratio is carried out according to the given dilution ratio parameters to obtain the diluted cell suspension. The samples prepared under this dilution ratio concentration have good uniformity, which can greatly improve the quality of the tablet.

To sum up, the present invention has the following substantive features and significant progress:

The cell suspension concentration measurement and liquid dosing device and working method of the present invention improve the quality of liquid-based thin-layer cytology preparations; the device uses a monochromatic LED as a light source, and a collimating lens is not needed to collimate the light source, and the A light guide tube of a certain length and diameter can realize the function of a collimating lens; no filter is required to filter out other wavelengths of light; The amount of sample is added to the cuvette for measurement, and the concentration value of the sample to be tested can be obtained.

The cell suspension concentration measurement and liquid dispensing device and working method of the present invention can well estimate the concentration value of the cell suspension to be measured, perform dilution and proportioning according to the given dilution and proportioning parameters, and obtain the diluted cell suspension, The samples prepared at this dilution ratio have good uniformity, which can greatly improve the quality of the tablet, and overcome the problems of large differences in the results of empty tablets, stripping, stacking and tableting, and poor consistency. .

The cell suspension concentration measurement and liquid dispensing device and working method of the present invention are finally applied to the filming process of the liquid-based thin-layer cytology tableting machine, which improves the tableting quality and improves the doctor's manual microscopic examination. Efficiency and interpretation accuracy of machine reading, especially the detection rate of cervical cancer cells.

The cell suspension concentration measurement and liquid dispensing device and working method of the present invention, if the cell suspension to be measured is the same type of cells, it is only necessary to set the parameters of the standard curve during initial use, and then it can be used directly.

Those skilled in the art can easily understand that the above are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention, etc., All should be included within the protection scope of the present invention.

Claims (10)

1. A cell suspension concentration measurement and liquid preparation device is characterized in that: comprises an MCU, a cell suspension concentration measuring device and a cell suspension liquid preparation device;

the cell suspension concentration measuring device comprises a monochromatic light source, a cuvette, a photoelectric detector, a cassette and a light guide cylinder; the monochromatic light source, the cuvette, the photoelectric detector and the light guide cylinder are arranged in the cassette, the cuvette is arranged between the monochromatic light source and the photoelectric detector, and the two sides of the cuvette are respectively provided with the light guide cylinders which are opposite and are respectively arranged between the monochromatic light source and the photoelectric detector;

the cell suspension liquid preparation device comprises a quantitative liquid taking device and a liquid taking rod; the quantitative liquid taking device is connected with the liquid taking rod, and the liquid taking rod comprises a liquid taking rod of a liquid to be detected and a diluent taking rod;

and the MCU is respectively connected with the monochromatic light source, the photoelectric detector, the light guide cylinder and the quantitative liquid taking device in a control mode.

2. The cell suspension concentration measuring and dispensing apparatus of claim 1, wherein:

the cell suspension concentration measuring device also comprises a light source driving device which is connected between the monochromatic light source and the MCU and is used for maintaining the stability of the light intensity of the light source and adjusting the light intensity.

3. The cell suspension concentration measuring and dispensing apparatus of claim 1, wherein:

the cell suspension concentration measuring device also comprises a signal amplifying device which is connected between the photoelectric detector and the MCU and used for collecting weak current signals generated by the photoelectric detector and converting the weak current signals into voltage signals which can be directly collected.

4. The cell suspension concentration measuring and dispensing apparatus of claim 1, wherein:

the cell suspension concentration measuring device further comprises a cassette cover mounted on the cassette; when the cassette cover is opened, the cuvette is taken and placed from the cassette.

5. The cell suspension concentration measuring and dispensing apparatus of claim 1, wherein:

the quantitative liquid taking device of the cell suspension liquid preparation device is a microliter-level flow liquid transfer pump.

6. The cell suspension concentration measuring and dispensing apparatus of claim 5, wherein:

the microliter-level flow liquid-moving pump is respectively connected with the liquid-taking rod of the liquid to be detected and the diluent liquid-taking rod through silicone tubes.

7. The cell suspension concentration measuring and dispensing apparatus of claim 1, wherein:

the cell suspension concentration measuring and liquid preparing device further comprises a display screen which is connected with the MCU and used for operation prompt and information display in the measuring and liquid preparing process, a user interaction interface is provided, and the use by an operator is facilitated.

8. A method of operating a cell suspension concentration measuring and dispensing apparatus according to any one of claims 1-7, comprising the steps of:

s1, determining a cell suspension absorption spectrum curve to obtain a maximum absorption wavelength, injecting a plurality of groups of cell suspension samples with different concentrations into a cuvette by adopting a monochromatic light source with the wavelength, sequentially measuring the transmission light intensity of light emitted by the monochromatic light source after the light penetrates through the cuvette and the cell suspension samples in the cuvette, and converting the transmission light intensity into absorbance so as to obtain a standard curve of the relation between the concentration and the absorbance;

s2, placing the empty cuvette into a cassette, and performing zero calibration before measurement by an MCU;

s3, sucking the fully and uniformly mixed cell suspension to be detected, injecting the cell suspension to be detected into an empty cuvette, measuring the transmission light intensity of the cell suspension to be detected, converting the transmission light intensity into absorbance, and substituting the absorbance into a standard curve of the relation between concentration and absorbance to obtain the concentration of the cell suspension to be detected;

s4, calculating and controlling respective sampling amounts of the liquid taking rod of the liquid to be detected and the liquid taking rod of the diluent according to the measured concentration of the cell suspension liquid to be detected and preset dilution ratio parameters, mixing and fully shaking up to obtain diluted mixed liquid for tabletting, and then using the diluted mixed liquid for tabletting;

s5, replacing the cuvette, repeating the steps S2-S4, and carrying out concentration measurement and solution preparation on the next cell suspension to be detected.

9. The method of operating a cell suspension concentration measuring and dispensing apparatus of claim 8, wherein:

in step S3, the burette is used to suck the fully mixed cell suspension to be tested, and the burette nozzle is attached to the inner wall of the empty cuvette to slowly extrude the cell suspension to be tested in the burette, thereby avoiding the generation of bubbles.

10. The method of operating a cell suspension concentration measuring and dispensing apparatus of claim 8, wherein:

in step S4, connecting the liquid taking rod of the liquid to be detected with a disposable liquid transfer gun head, putting the liquid taking rod into the cell suspension liquid to be detected which is fully and uniformly mixed in step S3, stopping sampling after the MCU controls the calculated sampling amount, putting the gun head into an empty test tube, and removing the gun head after the liquid taking rod is discharged completely; and (4) adopting a diluent taking rod to absorb the diluent with the calculated sampling amount, putting the diluent into the same test tube, and fully shaking up the test tube.

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