CN111265677A - Detection liquid based on ionic conduction, preparation method thereof, use method for bioelectrical impedance measurement and application - Google Patents
Detection liquid based on ionic conduction, preparation method thereof, use method for bioelectrical impedance measurement and application Download PDFInfo
- Publication number
- CN111265677A CN111265677A CN202010204750.6A CN202010204750A CN111265677A CN 111265677 A CN111265677 A CN 111265677A CN 202010204750 A CN202010204750 A CN 202010204750A CN 111265677 A CN111265677 A CN 111265677A
- Authority
- CN
- China
- Prior art keywords
- detection liquid
- ionic conduction
- bioelectrical impedance
- preparation
- conductivity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
- C09D5/00—Coating compositions, e.g. paints, varnishes or lacquers, characterised by their physical nature or the effects produced; Filling pastes
- C09D5/24—Electrically-conducting paints
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0033—Features or image-related aspects of imaging apparatus, e.g. for MRI, optical tomography or impedance tomography apparatus; Arrangements of imaging apparatus in a room
- A61B5/004—Features or image-related aspects of imaging apparatus, e.g. for MRI, optical tomography or impedance tomography apparatus; Arrangements of imaging apparatus in a room adapted for image acquisition of a particular organ or body part
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/05—Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; Measuring using microwaves or radio waves
- A61B5/053—Measuring electrical impedance or conductance of a portion of the body
- A61B5/0536—Impedance imaging, e.g. by tomography
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Engineering & Computer Science (AREA)
- Radiology & Medical Imaging (AREA)
- Medical Informatics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Pathology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Materials Engineering (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
Abstract
A detection liquid based on ionic conduction, a preparation method thereof, a use method for bioelectrical impedance measurement and application thereof belong to the technical field of medicine manufacturing. The detection liquid is ionic conductive liquid, and the ions are Na+、K+、H+、CL‑、SO4 2‑、PO4 3‑、CH3COO‑The solvent is water. The detection liquid based on ionic conduction, the preparation method thereof, the use method for bioelectrical impedance measurement and the application thereof can ensure that the measurement error of a plurality of electrodes is less than 1 percent and even less, the detection liquid does not deteriorate, does not breed bacteria, does not change the conductivity, is nontoxic and harmless, does not generate electrochemical reaction in the measurement process, does not generate substances or gases harmful to human bodies, and is easy to obtain raw materialsSafety, environmental protection, low production cost and good repeatability.
Description
Technical Field
The invention belongs to the technical field of medicine manufacturing, and particularly relates to detection liquid based on ionic conduction, a preparation method thereof, a use method for bioelectrical impedance measurement and application thereof.
Background
Bioelectrical Impedance Tomography (EIT) technology, Electrical Impedance scanning imaging (EIS) technology and a novel medical functional imaging technology are adopted, and the principle of the novel medical functional imaging technology is that a weak current or a weak voltage is applied to a human body surface electrode, voltage values or current values on other electrodes are measured, and the internal Electrical Impedance value or the change value of the Electrical Impedance of the human body is reconstructed according to the relation between the voltage and the current. The method has the advantages of repeated measurement, high imaging speed, functional imaging and the like because nuclides or rays are not used and are harmless to a human body, and the method has the characteristics of low cost and no requirement on a special working environment, so that the bioelectrical impedance measurement imaging technology is an ideal nondestructive medical imaging technology with wide application prospect.
Since the discovery of the invention in 1982, the electrical impedance measurement imaging technology has proved the possibility of being successfully applied to two fields of industry and medicine in theory, and can be applied to the detection of the heart and lung functions, the skull, the mammary gland, the limbs and other organs of the human body in the medical field. In addition to the application to the detection of each limb of the human body, the electric field CT represented by the impedance CT has not been able to be cited as one of the major clinical obstacles, namely, the accuracy of the measured data cannot reach the accuracy and reliable repeatability required by the clinic.
For example, in the measurement of structural and pathological changes that may occur in the human breast, it is required that the measurement system should achieve a combined measurement accuracy of at least 1%, preferably 1%, and more preferably one ten thousandth and more, by means of a multi-electrode sensor. The important process is that the contact area can not reach one thousandth by only depending on the processing precision of the electrodes, because in fact, when dozens of electrodes are used for simultaneous measurement, the contact area between the electrodes and the surface of a measured object can not ensure 100 percent of the area of the contact, and the contact impedance is related to the contact surface pressure and is also related to the contact surface cleanliness and the cuticle of the human body surface. This is one of the key reasons why the clinical application of electric field CT represented by impedance CT is severely limited, and the development of breast cancer monitoring or detecting products in many countries around the world is unable to progress.
The prior art has the following disadvantages: electrode systems, which are contact-based mechanisms, are subject to high errors, mainly beyond the precision required by clinical measurement systems. Therefore, it is often necessary: 1. a large contact area electrode system to reduce contact resistance and relative errors between electrodes in the introduced contact resistance; 2. the contact areas between all the electrodes are completely equal; 3. the pressure of all electrode contacts is completely the same; 4. and ensures that the electrodes are in full contact with the human body, namely 100 percent of contact, and the contact error which is required to be as large as the area between the electrodes cannot be controlled. How to overcome the error caused by the digital acquisition of the system sensor due to the contact of the electrodes becomes the most critical factor in clinical application and impedance CT. Since the invention of WO2007017634a2 patent, reliable electrode and media liquid manufacturing techniques and quality stability were used in the implementation. First, the metallic nature of the electrodes used ensures that the ions are able to perform a function equivalent to electronic conduction. Second, the ion concentration and the electric field formed by the electron response should reach the concentration of the signal-to-noise ratio of the bioelectronic signal that we need. At a specific temperature, the concentration of ions and the density of the electric field generated by the ions and electrons are both related to the temperature. The invention patent states that the key technology of bioelectrical impedance measurement imaging is ion conductive detection liquid, the ion conductive detection liquid is possible to have a multi-electrode detection error smaller than 1% or even one ten thousandth, the temperature fluctuation control is possible within the range of 0.1-0.5 ℃, and a stable electric field and uniform current distribution can be formed.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to design and provide detection liquid based on ionic conduction, a preparation method thereof, a use method for bioelectrical impedance measurement and a technical scheme of use thereof, wherein the detection liquid can ensure that the measurement error of a plurality of electrodes is less than 1% and even less, the detection liquid does not deteriorate and breed bacteria within 6 months after being used on a computer, the conductivity is unchanged, the detection liquid is nontoxic and harmless, the detection liquid does not produce electrochemical reaction in the measurement process, substances or gases harmful to human bodies are not produced, and the detection liquid is easy to obtain raw materials, safe, environment-friendly, low in production cost and good in repeatability.
The detection liquid based on ion conduction is characterized in that the detection liquid is ionic conductive liquid, and the ions are Na+、K+、H+、CL-、SO4 2-、PO4 3-、CH3COO-The solvent is water.
The preparation method of the detection liquid based on ion conduction is characterized by comprising the following steps: mixing sterilized purified water with a sterilization bacteriostat to obtain a mixed solution, wherein the mass concentration of the sterilization bacteriostat is 0.02-0.08%; then water-soluble salts or acids are used for blending until the conductivity is 300-1000 mu S/cm, and the mixture is sealed for standby; the bactericidal bacteriostatic agent is one or more of polyhexamethylene guanidine hydrochloride, ethanol, acetic acid and lactic acid, and the water-soluble salts or acids are one or more of sodium chloride, potassium chloride, sodium acetate, potassium acetate, sodium phosphate, potassium phosphate, sodium sulfate, potassium sulfate, hydrochloric acid, phosphoric acid and sulfuric acid.
The preparation method of the detection liquid based on ion conduction is characterized by comprising the following steps: weighing 15 liters of sterilized purified water, adding 7.5g of polyhexamethylene biguanide hydrochloride, adding reagent-grade sodium chloride, fully dissolving under stirring until the conductivity reaches 300us/cm, and sealing for later use.
The preparation method of the detection liquid based on ion conduction is characterized by comprising the following steps: weighing 15 liters of sterilized purified water, adding 150g of acetic acid, adding reagent-grade sodium acetate, fully dissolving under stirring until the conductivity reaches 900us/cm, and sealing for later use.
The preparation method of the detection liquid based on ion conduction is characterized by comprising the following steps: weighing 15 liters of sterilized purified water, adding 150g of phosphoric acid, adding reagent-grade potassium phosphate, fully dissolving under stirring until the conductivity reaches 500us/cm, and sealing for later use.
The preparation method of the detection liquid based on ionic conduction is characterized in that the mass concentration of the bactericidal bacteriostatic agent is 0.03-0.07%, preferably 0.04-0.06%, and more preferably 0.05%.
The preparation method of the detection liquid based on ionic conduction is characterized in that the conductivity of the detection liquid is as follows: 400-900. mu.S/cm, preferably 500-800. mu.S/cm, more preferably 600-700. mu.S/cm.
The use method of the ionic conduction-based detection liquid for bioelectrical impedance measurement is characterized in that: when the detection works, the frequency of the alternating current power supply is 100Hz-100MHz, the current is 0.1mA-1.0mA, and the voltage is 1.0-2.5V.
The use method of the ionic conduction-based detection liquid for bioelectrical impedance measurement is characterized in that: the frequency of the alternating current power supply is 300Hz-50MHz, preferably 500Hz-25 MHz; the current is 0.2mA-0.8mA, preferably 0.3mA-0.7mA, more preferably 0.4mA-0.5 mA; the voltage is 1.2-2.2V, preferably 1.5-2.0V, more preferably 1.6-1.8V.
The detection liquid based on ion conduction is applied to the preparation of system calibration detection liquid of a non-contact electrode system in EIS (electronic impedance spectroscopy) equipment specially applying bioimpedance to carry out medicine and biotechnology, multi-dimensional mammary gland electrical impedance mammary gland image analyzer equipment based on bioelectrical impedance tomography, and detection liquid of EIM (electronic impedance spectroscopy) random general investigation equipment for carrying out impedance measurement.
The detection liquid based on ionic conduction, the preparation method thereof, the use method for bioelectrical impedance measurement and the application thereof can ensure that the measurement error of a plurality of electrodes is less than 1 percent and even less, the detection liquid does not deteriorate within 1 month after being used on a machine, does not breed bacteria, has unchanged conductivity, is nontoxic and harmless, does not generate electrochemical reaction in the measurement process, does not generate substances or gases harmful to human bodies, has easily obtained raw materials, is safe and environment-friendly, has low production cost and good repeatability.
Detailed Description
The present invention will be further described with reference to specific examples thereof.
Example 1:
preparation of detection liquid: weighing 15 liters of sterilized purified water, adding 7.5g of polyhexamethylene biguanide hydrochloride, adding reagent-grade sodium chloride, fully dissolving under stirring until the conductivity reaches 300us/cm, and sealing for later use. The conductivity is measured by a conventional conductivity tester on the market.
Example 2:
preparation of detection liquid: weighing 15 liters of sterilized purified water, adding 150g of acetic acid, adding reagent-grade sodium acetate, fully dissolving under stirring until the conductivity reaches 1000us/cm, and sealing for later use.
Example 3:
preparation of detection liquid: weighing 15 liters of sterilized purified water, adding 150g of phosphoric acid, adding reagent-grade potassium phosphate, fully dissolving under stirring until the conductivity reaches 500us/cm, and sealing for later use.
Example 4:
preparation of detection liquid: weighing 15 liters of sterilized purified water, adding 4.5g of ethanol, adding reagent grade sodium sulfate, fully dissolving under stirring until the conductivity reaches 500us/cm, and sealing for later use.
Example 5:
preparation of detection liquid: weighing 15 liters of sterilized purified water, adding 10g of lactic acid, adding reagent-grade sodium phosphate, fully dissolving under stirring until the conductivity reaches 735us/cm, and sealing for later use.
Example 6:
preparation of detection liquid: weighing 15 liters of sterilized purified water, adding 6g of polyhexamethylene guanidine hydrochloride, adding reagent-grade potassium acetate or potassium chloride, fully dissolving under stirring until the conductivity reaches 600us/cm, and sealing for later use.
The purified water in examples 1 to 6 of the present invention may be distilled water or purified water. When the detection liquid prepared by the invention is used for human body detection, different conductivity can be adopted according to different people, the principle is to ensure the human body safety current, in this range, the concentration and the density of the current can be ensured, and the current can also be ensured not to be excessive to form local short circuit between two electrodes, namely, more than 99 percent of the current flows through an object to be detected and organs, and a very small amount of the current can flow through other unrelated electrodes, so that the precision of the whole detection system is controlled in a limited range, enough conductive ions form a steady-current field under a pressurized electric field for scanning the object to be detected by an electric field CT, the electric field of the whole object to be detected is in a dynamic relative stable state, and the whole measurement has very good repeatability. The detection liquid prepared by the invention does not deteriorate within 6 months before leaving a factory and being used, the microbial effect is not generated, the conductivity is not changed, and the biological side effect caused by the fact that microorganisms generated by a human body damage the human body in the using process is avoided.
The above description is only a preferred embodiment of the present invention, and for those skilled in the art, the present invention should not be limited by the description herein, since various changes and modifications can be made in the details of the embodiment and the application range according to the spirit of the present invention.
Claims (10)
1. The detection liquid based on ion conduction is characterized in that the detection liquid is ionic conductive liquid, and ions are Na+、K+、H+、CL-、SO4 2-、PO4 3-、CH3COO-The solvent is water.
2. The method for preparing an ionic conduction-based detection liquid according to claim 1, characterized by comprising the following steps: mixing sterilized purified water with a sterilization bacteriostat to obtain a mixed solution, wherein the mass concentration of the sterilization bacteriostat is 0.02-0.08%; then water-soluble salts or acids are used for blending until the conductivity is 300-1000 mu S/cm, and the mixture is sealed for standby; the bactericidal bacteriostatic agent is one or more of polyhexamethylene guanidine hydrochloride, ethanol, acetic acid and lactic acid, and the water-soluble salts or acids are one or more of sodium chloride, potassium chloride, sodium acetate, potassium acetate, sodium phosphate, potassium phosphate, sodium sulfate, potassium sulfate, hydrochloric acid, phosphoric acid and sulfuric acid.
3. The method for preparing an ionic conduction-based detection liquid according to claim 1, characterized by comprising the following steps: weighing 15 liters of sterilized purified water, adding 7.5g of polyhexamethylene biguanide hydrochloride, adding reagent-grade sodium chloride, fully dissolving under stirring until the conductivity reaches 300us/cm, and sealing for later use.
4. The method for preparing an ionic conduction-based detection liquid according to claim 1, characterized by comprising the following steps: weighing 15 liters of sterilized purified water, adding 150g of acetic acid, adding reagent-grade sodium acetate, fully dissolving under stirring until the conductivity reaches 900us/cm, and sealing for later use.
5. The method for preparing an ionic conduction-based detection liquid according to claim 1, characterized by comprising the following steps: weighing 15 liters of sterilized purified water, adding 150g of phosphoric acid, adding reagent-grade potassium phosphate, fully dissolving under stirring until the conductivity reaches 500us/cm, and sealing for later use.
6. The method according to claim 2, wherein the mass concentration of the antibacterial and bacteriostatic agent is 0.03-0.07%, preferably 0.04-0.06%, and more preferably 0.05%.
7. The method according to claim 2, wherein the conductivity of the detection liquid is as follows: 400-900. mu.S/cm, preferably 500-800. mu.S/cm, more preferably 600-700. mu.S/cm.
8. Use of an ionic conduction based detection liquid for bioelectrical impedance measurements according to claim 1, characterized in that: when the detection work is carried out, the frequency of the alternating current power supply is 100Hz-100MHz, the effective value of the current is 0.1mA-1.0mA, and the voltage is 1.0-2.5V.
9. The use of the ionic conduction-based detection liquid for bioelectrical impedance measurement according to claim 8, wherein: the frequency of the alternating current power supply is 300Hz-50MHz, preferably 500Hz-25 MHz; the current is 0.2mA-0.8mA, preferably 0.3mA-0.7mA, more preferably 0.4mA-0.5 mA; the voltage is 1.2-2.2V, preferably 1.5-2.0V, more preferably 1.6-1.8V.
10. Use of the ion-conducting detection liquid according to claim 1 or 2 for preparing EIS for medical and biotechnology by using bioimpedance, system calibration detection liquid for non-contact electrode system in multi-dimensional mammary gland electrical impedance breast image analyzer equipment by using bioelectrical impedance tomography, and detection liquid for EIM random census equipment for impedance measurement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010204750.6A CN111265677A (en) | 2020-03-22 | 2020-03-22 | Detection liquid based on ionic conduction, preparation method thereof, use method for bioelectrical impedance measurement and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010204750.6A CN111265677A (en) | 2020-03-22 | 2020-03-22 | Detection liquid based on ionic conduction, preparation method thereof, use method for bioelectrical impedance measurement and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111265677A true CN111265677A (en) | 2020-06-12 |
Family
ID=70992587
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010204750.6A Pending CN111265677A (en) | 2020-03-22 | 2020-03-22 | Detection liquid based on ionic conduction, preparation method thereof, use method for bioelectrical impedance measurement and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111265677A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007017634A2 (en) * | 2005-08-05 | 2007-02-15 | De Montfort University | An apparatus and method for non-contact electrical impedance imaging |
| CN104583118A (en) * | 2012-08-23 | 2015-04-29 | 独立行政法人科学技术振兴机构 | Carbon nanomaterial, composition, conductive material, and method for producing same |
-
2020
- 2020-03-22 CN CN202010204750.6A patent/CN111265677A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007017634A2 (en) * | 2005-08-05 | 2007-02-15 | De Montfort University | An apparatus and method for non-contact electrical impedance imaging |
| CN104583118A (en) * | 2012-08-23 | 2015-04-29 | 独立行政法人科学技术振兴机构 | Carbon nanomaterial, composition, conductive material, and method for producing same |
Non-Patent Citations (2)
| Title |
|---|
| 沈克琦 主编: "《高中物理学.3》", 31 July 2015, 合肥：中国科学技术大学出版社 * |
| 董秀珍 等: ""电阻抗断层成像系统及重构算法"", 《第四军医大学学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Naik et al. | Fabrication of poly (sudan III) modified carbon paste electrode sensor for dopamine: A voltammetric study | |
| JPS6356498B2 (en) | ||
| Wu et al. | Simultaneous voltammetric determination of epinephrine and acetaminophen using a highly sensitive CoAl-OOH/reduced graphene oxide sensor in pharmaceutical samples and biological fluids | |
| CN107957440B (en) | Planar ammonia selective sensing electrode and method for fabricating the same | |
| CN108195900B (en) | Electrochemical sensor with temperature compensated hematocrit test function | |
| US20230078221A1 (en) | Method for manufacturing microneedle biosensor | |
| CN106501332B (en) | Zn-MOFs/glassy carbon electrode and its fabrication method and application | |
| Manjunatha et al. | Electrocatalytic response of dopamine at mannitol and Triton X-100 modified carbon paste electrode: A cyclic voltammetric study | |
| Kumar et al. | Ammonium heptamolybdate preloaded on flexible carbon-matrix film electrode for the electrochemical phosphate sensor in a river water sample | |
| EP3357414A1 (en) | Biosensor for detecting biological fluid | |
| CN111265677A (en) | Detection liquid based on ionic conduction, preparation method thereof, use method for bioelectrical impedance measurement and application | |
| Tan et al. | Production of Ni 7 S 6/NiO hybrids as a highly sensitive amperometric sensor for glucose | |
| CN111307890B (en) | Manganese oxide/graphene modified electrode and method for detecting salicylic acid in plant tissue based on manganese oxide/graphene modified electrode | |
| Zeng et al. | A bioinspired flexible sensor for electrochemical probing of dynamic redox disequilibrium in cancer cells | |
| CN104897755B (en) | One kind is based on the pH detecting electrodes of graphene/PEDOT composite membranes | |
| CN217156402U (en) | Electrochemical sensor based on associated sensor | |
| Zhao et al. | Radiation-tolerant thin-film reference electrodes and potentiometric sensors | |
| CN109813788A (en) | A kind of ion selective electrode of liquid intermediate ion Concentration Testing, measuring appliance and its preparation method and application | |
| Wang et al. | Electrochemical determination of diethylstilbestrol in animal food using a poly polylysine/graphene modified electrode | |
| CN113219026A (en) | Silk-screen printing electrode for quickly detecting uric acid | |
| CN113916955A (en) | Preparation and detection method of a photoelectrochemical biosensor for detecting choline in infant formula milk powder | |
| Yohannes et al. | Differential pulse stripping voltammetric determination of paracetamol in pharmaceutical tablet samples using murexide modified carbon paste electrode | |
| Emde | Electrochemical aspects of pH electrodes | |
| Liao et al. | Potentiometric multisensor based on ruthenium dioxide thin film with a Bluetooth wireless and Web-based remote measurement system | |
| KR20210046159A (en) | lactate sensor system using phase change |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200612 |