CN111265224B - Blood collection aid and method for separating a serum sample from whole blood - Google Patents

Abstract

The invention relates to a lancing aid for separating a serum sample from whole blood, comprising at least: the blood collection tube body is used for storing collected liquid; a blood collection tube cap assembled to the blood collection tube body; and a blood collection dispensing assembly that controllably delivers liquid within the blood collection tube body to an exterior of the blood collection tube body by way of one end of the blood collection dispensing assembly being fittingly connectable to the blood collection tube cap, the blood collection dispensing assembly being configured to: the blood collection dispensing assembly is configured to cause the liquid stored in the blood collection tube body to be output in prescribed amounts in divided portions in such a manner that the gas from the outside of the blood collection tube body via the blood collection tube cap does not pass through the liquid stored in the blood collection tube body.

Description

Blood collection aid and method for separating serum samples from whole blood

technical field

The invention relates to the technical field of blood processing, in particular to an auxiliary blood collection device and method for separating serum samples from whole blood.

Background technique

Blood is composed of two parts, plasma and blood cells. In the body, it is closely connected with various tissues and organs of the whole body through the circulatory system, participates in various functional activities of the body, and plays an important role in maintaining the body's metabolism, function regulation, and balance of the body's internal and external environments. Under pathological conditions, blood system diseases can not only directly affect the blood, but also affect the tissues and organs of the whole body, and tissue and organ lesions can also directly or indirectly cause changes in blood components. Blood tests can not only reflect the lesions of the blood system itself, but also assist Diagnose the disease, judge the progress of the patient's condition, and provide a reference for the treatment of the disease. Therefore, blood test is one of the commonly used test items in clinical practice. The veins in the elbow fossa of most people are thick and shallow, so it is easy to find blood vessels and perform puncture. In addition, the sensitivity of the skin at the fossa of the elbow is lower than that of other parts, and the pain is relatively weak when drawing blood. Venous blood volume is slower, venous blood pressure is lower than arterial blood pressure, vein wall is thinner than arterial wall, and most veins are located on the superficial skin, etc. Of course, arteries also have superficial surfaces, but most of them are relatively deep. For safety reasons, most Blood collection is venous blood. Venous blood sample collection refers to the method of drawing blood samples from veins. The veins used in the collection of venous blood samples usually include the basilic vein, median cubital vein, wrist and dorsal vein, great saphenous vein, small saphenous vein, dorsalis pedis vein, external jugular vein (multiple choices for infants), and femoral vein. .

Blood collection by venipuncture and intravenous infusion are widely used in clinical work, mainly for laboratory examination and drug treatment. As the main means of laboratory examination, venous blood collection is also an important part of clinical nursing work. The key measure is that during the long-term infusion process, various factors such as uncomfortable placement of limbs and limited activities of the subject to be blood-collected can easily cause the puncture needle to move and pierce the blood vessel, and the liquid will leak out, causing redness or necrosis of the skin, increasing patient suffering. Especially in the blood collection process, it is necessary to expose some limbs of the blood collection subject, but in autumn and winter, the indoor temperature is low, which is not conducive to blood vessel filling; usually a simple mat is placed under the operation site or placed directly on the operation table, which feels uncomfortable and not easy. Fixed; during intravenous infusion of children and critically ill patients, splints, bandages and other equipment are required to protect the infusion, which is inconvenient for patients; the traditionally used rubber tube cuff is thinner, and when the skin is tightened, the contact area with the skin is small, and the subject to be blooded feels pain Obviously, when the skin is dry, it is easy to loosen, and the pressure is not easy to control. It is easy to cause subcutaneous congestion and swelling when it is applied, and it is very easy to cause complications such as nerve paralysis, and at the same time it increases the difficulty of work.

The operation steps of the venous blood collection method: 1. Select the appropriate container according to the inspection purpose. Check the integrity of the container and affix the inspection label on the outside of the container. 2. Check the syringe: open the disposable syringe package, hold the needle seat in the left hand, and the syringe barrel in the right hand, connect the needle and the syringe tightly, and align the slope of the needle with the scale of the syringe, pull the needle plug to check whether there is blockage or leakage. gas. Finally exhaust the air in the syringe and set aside. 3. Select the vein: the subject to be blood-collected takes a sitting position, with the forearm stretched horizontally and placed on the desktop pillow. Expose the puncture site and select a suitable blood vessel, usually the median cubital vein, cephalic vein or basilic vein. Instruct the patient to make a fist to fill the vein. 4. Disinfection: first use a sterile cotton swab to disinfect the skin from the selected venipuncture site from the inside to the outside in a clockwise direction, and wait for it to dry. 5. Tie a cuff: Tie a tourniquet at 6 cm above the puncture site, and ask the blood collection subject to clench his fist several times, so that the vein is filled and exposed for easy puncture. 6. Puncture after re-disinfection: remove the sterile cap of the needle, fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe barrel with the thumb and middle finger of the right hand, fix the lower seat of the needle with the index finger, make the bevel of the needle and the scale of the barrel upward, and follow the direction of the vein Insert the needle at an angle of 15° to 30° between the needle and the blood vessel. After seeing the return of blood, insert the needle a little in order to prevent the needle from slipping out during blood collection; but do not puncture deeply with force to avoid hematoma, and remove the cuff immediately. 7. Blood drawing: fix the syringe with the left hand, slowly pump the inner core of the syringe to the required blood volume, press the needle hole with a sterile dry cotton ball, wait for the blood collection subject to loosen his fist, and quickly pull out the syringe. Instruct the subject to take blood to press the part with a sterile dry cotton swab for 1-2 minutes. 8. Bleeding and mixing: Remove the needle of the syringe, and slowly inject the blood into the anticoagulant tube along the wall of the test tube to prevent hemolysis and foam generation. If there is a test tube with anticoagulant, shake the test tube gently to fully mix the anticoagulant with the blood, and close the stopper of the test tube tightly for later use.

Vacuum blood collection technology used in venous blood collection was invented in the late 1930s. This technology has the advantages of convenient clinical use, simple operation, fully sealed test tube, biological safety, easy repeated inspection of specimens, and easy preservation of specimens. It is basically popularized and applied throughout the country. Laboratory vacuum blood collection tube specimens generally require processing before analysis, such as biochemical specimens, which go through the steps of collection, centrifugation, cap opening, inspection, cap closure, preservation, and disinfection. The entire process is labor-intensive and cumbersome, so the process needs to be optimized , Improve efficiency and reduce labor intensity. Vacuum blood collection tube technology is currently the most advanced blood collection technology in the world. The technical characteristics of vacuum blood collection tubes are: (1) Automatic measurement, no need to withdraw: due to the artificial vacuum in the blood collection tube, the blood sampling volume can be controlled by controlling the air pressure. There is no need to manually draw back and measure during operation; (2) High degree of sterility and small inspection interference: In order to maintain the vacuum in the tube, the vacuum blood collection tube cap has better airtightness, high degree of sterility, and a small chance of blood contamination, which has little effect on the test results. (3) Once blood is injected into the tube, the change of blood composition is small: the vacuum blood collection method directly injects blood into the blood collection tube, omitting the two processes of blood staying in the syringe barrel and transferring from the syringe to the blood collection tube, The repeated extrusion of blood is reduced, the destruction of blood cells is less, and the test results are more reliable.

At present, there are two types of blood collection tubes: soft-connected two-way blood collection needle system (scalp vein two-way blood collection type) and hard-connected two-way blood collection needle system (sleeve two-way blood collection type)), both of which are puncture needles at one end and puncture plugs at the other end Needle. Because the vacuum blood collection method complies with biosafety measures, and is easy to operate, accurate and reliable, it is widely used in clinical practice. Vacuum blood collection technology is an advanced blood collection technology that is very popular among hospitals and patients. Using a disposable vacuum blood collection device instead of a disposable syringe for blood collection, due to its advantages of simple operation, less pain for patients, high work efficiency, unlimited blood collection volume, protection of operators and reduction of environmental pollution, it is more and more clinically used. widely.

In the actual operation of sample collection using a vacuum blood collection device, it is different from the requirements of different biochemical tests. Different biochemical tests require different amounts of samples to be collected, that is, the collected samples need to be quantitatively packaged. For example, in a genetic testing laboratory, separation of serum and plasma is a pre-order step of many testing steps, such as the method for collecting and quality control DNA samples in the detection of fetal chromosomal aneuploidy proposed by the publication number CN108486217A, which includes sample Collection steps, specifically: Blood collection tube 1 collects 5.0 mL of pregnant women’s peripheral blood. After the collection, gently invert the blood collection tube 4 times and place the blood collection tube in a refrigerator at 4.0°C in time. Plasma and serum separation must be performed within 8 hours; blood collection tube 2 Collect 10.0mL of peripheral blood from pregnant women. After the collection, gently invert the blood collection tube 10 times and store it at room temperature. Plasma and serum must be separated within 72 hours; , centrifuge for 15 minutes, absorb the supernatant plasma, transfer it to a 2.0mL EP tube placed on an ice box, and mark the corresponding sample number; pre-cool the high-speed centrifuge to 4.0°C, and after the temperature is stable, put it into the 2.0 mL tube obtained in the previous step. Centrifuge the mLEP tube for 10 minutes, point the tip at the non-white blood cell precipitate, draw the supernatant plasma on the ice box, divide it into 1.5mLEP tubes placed on the ice box, transfer 400uL plasma into each tube, and mark the sample number and Plasma tubes should be stored in -20.0°C or -80.0°C freezer immediately.

For example, in the above-mentioned sample quantitative subpackaging and processing operations, medical personnel usually obtain serum by first opening the cover and then sucking it through a straw. However, at present, most of the vacuum blood collection tubes in medical laboratories are manually opened by medical staff. There is no medical staff to close the caps on the patient specimens that have completed the inspection work, and there is no instrument for closing the caps on the patient specimens that have completed the inspection work. equipment, and the operation of manually opening the blood collection tube cover has the following disadvantages: 1. The labor is cumbersome; 2. It takes a long time; 3. The biological safety risk is high. The sudden release of the blood sample will form blood splash and generate aerosol, which will cause bacteria and viruses to infect the medical staff; in addition, the labor intensity of opening the vacuum blood collection tube and closing the blood collection tube cap after the inspection work is greater, and it is easier for the operator. Cause biological hazards; 4. In drug clinical trials, blood samples need to be divided into detection tubes and backup tubes after being collected and centrifuged. When blood samples are divided, the blood will be exposed to the air. Inaccurate test results not only increase the difficulty of work for medical staff, but also bring certain safety hazards to patients.

Aiming at the above-mentioned problems existing in the current sample quantitative subpackaging operation, many solutions have been proposed in the prior art. For example, the patent document with the publication number CN108601565A discloses a method for separating serum or plasma from whole blood samples. Apparatus and method for blood cell separation, which uses a cap with a reservoir such that when a sample container is centrifuged, blood cells are filled into the cap, and when the cap is removed, the blood cells are also removed and the serum or plasma is left behind In sample tubes, where serum or plasma can be easily aspirated by a pipette that reaches all the way to the bottom of the sample tube, thereby minimizing dead volume.

The above-mentioned patent proposes a separation device different from the conventional structure of the existing blood collection tube, but because it needs to completely replace the existing blood collection tube, it is not suitable for the existing conventional blood collection tube, which greatly increases the cost of blood sample experiments; and the above-mentioned patent The sample quantitative dispensing method adopted is still the method of fractional sampling by pipette in the existing conventional sampling method, resulting in the actual use of the separation device proposed in the above patent, within the sampling time after opening the cover, the blood The samples are always exposed to the external environment; in addition, the tiny amount of serum samples required for blood sample experiments is difficult to obtain accurately by pipette.

In addition, on the one hand, due to differences in the understanding of those skilled in the art; on the other hand, due to the fact that the inventor has studied a large number of documents and patents when making the present invention, but due to space limitations, all details and contents have not been listed in detail, but this is by no means The present invention does not possess the characteristics of these prior art, on the contrary, the present invention already possesses all the characteristics of the prior art, and the applicant reserves the right to add relevant prior art to the background technology.

Contents of the invention

In view of the shortcomings of the existing technology, such as the unsafe opening of the blood collection tube and the cumbersome sampling operation in the quantitative sample packaging operation of the vacuum blood collection device, a lot of problems have been proposed in the prior art to solve the problem of how to open the cover safely solutions, such as a supporting uncapping device with a complex structure and high operational precision and blood collection tube caps specially adapted to the uncapping device, the above solutions inevitably increase the cost of blood samples and the complexity of the operation process, and As a result, the serum sample after opening the cover is exposed to the external environment for a long time. Different from the prior art, the present invention, on the basis of satisfying the batched output requirements and quantitative requirements of the sample quantitative sub-packaging operation, proposes that the blood sample can be obtained without opening the cover and prevents the formation of unfavorable adsorption between gas and liquid. This solution is dedicated to improving the existing sampling operation device and process in a small range to provide a blood collection auxiliary device and method for separating serum samples from whole blood. The blood collection auxiliary device is used in combination with the existing conventional blood collection tubes, without abandoning the existing conventional blood collection tubes, thereby greatly reducing the cost required for blood sample experiments.

A blood collection auxiliary device for separating serum samples from whole blood, at least comprising: a blood collection tube body for storing collected liquid; a blood collection tube cover, which is assembled on the blood collection tube body; and a blood collection tube A packaging assembly, which can controllably deliver the liquid in the blood collection tube body to the outside of the blood collection tube body by means of one end of which is fitly connected with the blood collection tube cap, and the blood collection subpackaging assembly The liquid stored in the body of the blood collection tube is configured to output the liquid stored in the body of the blood collection tube in predetermined amounts in batches such that gas from outside the body of the blood collection tube via the cap of the blood collection tube does not pass through the liquid stored in the body of the blood collection tube.

Preferably, the blood collection sub-packaging assembly is configured to be adapted to the cap of the blood collection tube in a manner that limits the opening degree of the cap of the blood collection tube when the tube body of the blood collection tube is in the first placed state connected so that one end of the liquid channel and one end of the gas channel on the blood collection subpackage assembly communicate with the inside of the tube body of the blood collection tube respectively. Or, when the tube body of the blood collection tube is in the second placement state opposite to the first placement state, a predetermined amount of gas is allowed to pass through the gas passage without forming an adsorption effect between it and the liquid way into the inside of the tube body of the blood collection tube, so as to distribute a prescribed amount of liquid to the outside of the tube body of the blood collection tube.

According to a preferred embodiment, the body of the blood collection tube is provided with a hollow plate, and the hollow plate is configured to: allow the gas channel when the blood collection subpackage assembly is fitted and connected with the blood collection tube cover communicate with its inner cavity. and make the first end of the gas channel continue to extend along the inner cavity of the hollow plate in such a way that the gas from the outside of the blood collection tube body through the blood collection tube cover does not pass through the liquid stored in the blood collection tube body, and The open end of the hollow plate located on a different imaginary horizontal plane from the first end communicates with the inside of the tube body of the blood collection tube.

Preferably, the hollow plate is assembled to the inner wall of the blood collection tube body close to the cap of the blood collection tube in a movably connected manner. The hollow plate is configured to allow the gas channel to communicate with its inner cavity when the blood collection sub-packaging assembly is fitted and connected with the blood collection tube cap. The hollow plate is configured such that the first end of the gas channel continues along the inner cavity of the hollow plate. The hollow plate is configured to communicate with the inside of the tube body of the blood collection tube at an open end of the hollow plate that is on a different imaginary level from the first end. The hollow plate is configured to move in a direction away from the blood collection tube cover in response to the displacement of the gas channel relative to the blood collection tube cover in the blood collection tube body, so that When the blood vessel body is in the second placement state and the liquid is located above the cap of the blood collection tube, the open end of the hollow plate is located above the liquid level of the liquid.

According to a preferred embodiment, the hollow plate is assembled on the tube body of the blood collection tube before the blood collection operation is performed by using the tube body of the blood collection tube, wherein at least one first Two ends of a through hole communicate with the inside of the tube body of the blood collection tube respectively and are isolated from the inner cavity of the hollow plate. so that when the blood collection tube body is used for blood collection operation and/or when the blood collection tube body is converted to the second placement state, the liquid entering the blood collection tube body passes through at least one of the first The through hole flows freely inside the blood collection tube body and covers the inner wall of the blood collection tube cover.

Preferably, the two ends of the at least one first through hole provided on the hollow plate respectively communicate with the inside of the tube body of the blood collection tube and are isolated from the inner cavity of the hollow plate, so that When the blood collection tube body is used for blood collection operation and/or when the blood collection tube body is converted to the second placement state, the liquid entering the blood collection tube body passes through at least one of the first channels hole and flow freely inside the tube body of the blood collection tube, or the gas entering the lumen through the gas channel is restricted to flow along the direction of extension of the lumen, or the gas in the tube body of the blood collection tube is insoluble in the Liquids and solids are confined within the one-sided area defined by the hollow plate.

According to a preferred embodiment, the blood collection auxiliary device at least includes a pressurized gap, the pressurized gap is formed when the blood collection sub-packaging assembly is fitted and connected with the blood collection tube cover for The area where the gas channel provides a specified amount of gas. Wherein, when gas from the outside of the blood collection tube body enters the inside of the blood collection tube body, the air pressure in the pressurized gap and the air pressure in the blood collection tube body can quickly reach equilibrium.

Preferably, the pressurized gap is located between the blood collection sub-packaging assembly and the blood collection tube cover, which are hermetically connected to each other. The gas passage is assembled to the blood collection sub-packaging assembly in such a manner that the second end thereof away from the tube body of the blood collection tube is open in the pressurized gap. The liquid channel is assembled to the blood collection sub-package assembly in such a manner that its two ends respectively pass through the pressurized gap. Therefore, the gas delivered to the pressurized gap is input into the tube body of the blood collection tube through the gas channel and the hollow plate. When the input gas enters the inside of the tube body of the blood collection tube in a manner that does not form adsorption between it and the liquid, the air pressure in the pressurized gap and the air pressure in the tube body of the blood collection tube Able to reach equilibrium quickly.

According to a preferred embodiment, the blood collection auxiliary device includes at least a liquid level monitoring component, wherein the blood collection auxiliary device is configured to: Liquid flow between different liquid level positions monitored at different times. It can be determined whether the flow rate of the liquid distributed to the outside of the tube body of the blood collection tube satisfies a prescribed amount.

Preferably, the liquid level monitoring component is configured to be relatively fixed with the tube body of the blood collection tube for monitoring the position of the liquid level inside the tube body of the blood collection tube. The liquid level monitoring component monitors a first liquid level position at a first moment and a second liquid level position at a second moment, and is located between the first liquid level position and the second liquid level position The liquid flow rate and the specified amount of gas input along the gas channel are used to determine whether the liquid flow rate distributed to the outside of the tube body of the blood collection tube meets the specified amount.

According to a preferred embodiment, the blood collection auxiliary device is configured to: obtain the third liquid level position and the fourth liquid level collected by the blood collection auxiliary device when the blood collection tube body is in the second placement state. The surface position is used to determine the total amount of liquid collected by the tube body of the blood collection tube so that the user can determine at least one distribution method of the liquid based on the total amount of liquid.

Preferably, the blood collection auxiliary device is configured to determine whether to use the blood collection tube based on the height difference between the third liquid level position and the fourth liquid level position and the cross-sectional area of the blood collection tube body The total amount of liquid collected by the tube allows the user to determine at least one distribution method of the liquid based on the total amount of liquid.

According to a preferred embodiment, part of the surface of the hollow plate extends away from the cap of the blood collection tube, so that the inner cavity of the hollow plate extends along the central axis of the blood collection tube body. The open end of the hollow plate is located on a different imaginary horizontal plane from the surface of the hollow plate.

Preferably, the gas channel is configured to allow the gas input along the air pressure gap to pass through and isolate the liquid inside the tube body of the blood collection tube, so that the liquid inside the tube body of the blood collection tube can only pass through the liquid The channel outputs a predetermined amount to the outside of the tube body of the blood collection tube.

A blood collection auxiliary system for separating serum samples from whole blood, at least comprising: a blood collection tube body for storing collected liquid; a blood collection tube cap, which is assembled on the blood collection tube body; and a blood collection tube An assembly assembly, which can controllably transport the liquid in the tube body of the blood collection tube to the outside of the tube body of the blood collection tube by means of one end of which is fitly connected with the cap of the blood collection tube, and the blood collection auxiliary system is It is configured that when the blood collection sub-packaging assembly is fitted and connected with the blood collection tube cover to form a region for supplying a specified amount of gas to the gas channel, the gas from the outside of the blood collection tube body through the blood collection tube cover The gas does not pass through the liquid stored in the tube body of the blood collection tube, so that the liquid stored in the tube body of the blood collection tube is output in batches in a prescribed amount.

According to a preferred embodiment, the blood collection auxiliary system at least includes a hollow plate arranged in the tube body of the blood collection tube, and the hollow plate is configured to: allow the gas passage between the blood collection sub-packaging assembly and the blood collection tube The cap of the blood vessel communicates with its inner cavity when it is fitted and connected. And make the first end of the gas channel continue to extend along the inner cavity of the hollow plate in such a way that the gas from the outside of the blood collection tube body through the blood collection tube cover does not pass through the liquid stored in the blood collection tube body.

According to a preferred embodiment, the auxiliary blood collection system includes at least a liquid level monitoring component, wherein the auxiliary blood collection system is configured to The liquid flow between different liquid level positions monitored at all times. It can be determined whether the flow rate of the liquid distributed to the outside of the tube body of the blood collection tube satisfies a prescribed amount.

The blood collection auxiliary device and method for separating serum samples from whole blood provided by the present invention have at least the following beneficial technical effects:

The present invention proposes a solution based on the ability to obtain blood samples without opening the cover. The blood collection auxiliary device provided by the present invention is provided with a blood collection subpackage assembly that is compatible with the existing conventional structure without changing The existing conventional structure of the blood collection tube and the tube cap can be simply assembled after the blood collection is completed, and the serum sample inside the blood collection tube can be subpackaged. The operation process is simple and the cost of the blood sample is low. The exposure time of the serum sample is exposed to the external environment; and, by setting the hollow plate used to cooperate with the blood collection sub-package assembly used to provide gas, not only can effectively prevent the possibility of adsorption between gas and liquid, At the same time, reliable sampling efficiency and sampling volume accuracy are guaranteed.

Description of drawings

Fig. 1 is a schematic diagram of a simplified cross-sectional structure of a blood collection auxiliary device according to a preferred embodiment of the present invention;

Fig. 2 is a simplified structural schematic diagram of a hollow plate provided by the present invention;

Fig. 3 is a schematic structural view of the preferred connection mode between the gas channel and the hollow plate provided by the present invention;

Fig. 4 is a schematic diagram of a simplified module connection relationship of an auxiliary operation device according to a preferred embodiment of the present invention; and

Fig. 5 is a schematic diagram of a simplified structural connection relationship of a blood collection support pad according to a preferred embodiment of the present invention.

List of reference signs

1: Blood collection tube body 2: Blood collection tube cover 3: Blood collection dispensing assembly

4: Gas channel 5: Liquid channel 6: Hollow plate

7: Pressure gap 8: Liquid level monitoring component 9: First through hole

10: Through hole 11: First slider 12: Second slider

13: Solid component layer 14: Liquid component layer 15: Gas layer

16: Inert separation glue layer 101: Blood collection support pad 102: Blood vessel visualization equipment

103: Central processing module 104: Support plane 105: Airbag assembly

106: Cuff

Detailed ways

The present invention will be described in detail below in conjunction with the accompanying drawings.

Example 1

As shown in Figure 1, the present invention provides a blood collection aid for separating a serum sample from whole blood.

The blood collection auxiliary device includes a blood collection tube body 1 and a blood collection tube cap 2 .

The above blood collection tube body 1 and blood collection tube cover 2 are the most commonly used blood collection tube structures in the prior art:

The blood collection tube body 1 is used to store the collected liquid.

The cap 2 of the blood collection tube is assembled on the end of the tube body 1 of the blood collection tube so that the inside of the tube body is relatively sealed.

The blood collection tube cover 2 is detachably assembled on the blood collection tube body 1 , and serum samples can be collected from the blood collection tube body 1 through the pierceable layer above the blood collection tube cover 2 .

The pierceable layer is a common pharmaceutical grade butyl rubber stopper. The crown of the butyl rubber stopper is provided with a thinner part (the thickness of which is generally 2.7 mm to 3 mm) for the needle body to smoothly puncture the rubber stopper.

Below technical problem of the present invention and invention point are described in detail as follows:

The collected blood samples need to be transferred from the blood collection tube to different vessels for blood samples several times. In the existing blood sample transfer operation, the operator usually removes the blood collection tube cap 2 from the top of the blood collection tube body 1 before transferring the blood sample. For existing blood sample transfer operations:

On the one hand, since the inside of the blood collection tube body 1 is relatively sealed after the blood sample is collected, a certain air pressure is likely to be generated inside the blood collection tube body 1, and it is difficult to avoid the possibility of infectivity when the blood collection tube cover 2 is opened. The blood sample of the virus splashes out, and at the same time, the slender and elongated blood collection tube body 1 made of glass is prone to damage under the operation of forcefully opening the cover.

In the prior art, a large number of solutions for blood collection tubes have been proposed to improve the above shortcomings. The solutions focus on solving the problem of how to open the cap safely, and propose a supporting cap opening device with complex structure and high operation requirements and specially adapted to The blood collection tube cap 2 of the cap opening device greatly increases the cost of the blood sample and the complexity of the operation process, and requires the serum sample to be exposed to the external environment for a long time after the cap is opened.

Different from the prior art, the present invention proposes a solution based on the ability to obtain blood samples without opening the cover. The blood collection auxiliary device provided by the present invention is provided with a blood collection tube that is compatible with the existing conventional structure. Dispensing component 3, without changing the existing conventional structure of blood collection tubes and tube caps, can be simply assembled after blood collection to subpackage the serum samples inside the blood collection tubes, not only the operation process is simple and the cost is low, no need to open the cap At the same time, it greatly shortens the exposure time of serum samples to the external environment.

On the other hand, the supporting uncapping device with complex structure and high operation precision required in the above-mentioned prior art and the blood collection tube cap 2 specially adapted to the capping device did not consider the possible problems after uncapping. The problem of splashing blood samples with infectious virus. This is because in the subsequent fractional sampling process, in the existing conventional sampling methods, such as the method of pouring fractional sampling, not only the operational accuracy is higher than that of safety cap removal, but it is also difficult to avoid Splashes of blood samples that may contain infectious virus;

In addition, for example, if the pipette is used for fractional sampling, if the pipette is too deep, solid matter at the bottom of the blood collection tube may be sucked out, or if the pipette is too shallow, it will not be able to draw a sufficient amount of serum samples. It is difficult to accurately obtain the required micro-serum samples by pipette.

Different from the prior art, since the present invention proposes a solution based on increasing the internal pressure of the blood collection tube body 1 to carry out fractional sampling, the blood collection subpackage assembly 3 is used to input pressure into the inside of the blood collection tube body 1 The gas can not only indirectly control the sampling volume of the output serum sample by controlling the input volume of the pressurized gas, but also avoids the problem of difficulty in controlling the penetration depth of the sampler in the prior art because the liquid and the solid are separated from each other during sampling.

Furthermore, although the above-mentioned technical solution of pressurized gas driving quantitative discharge of liquid belongs to the prior art means in the same field or corresponding field, it cannot be directly combined with the blood collection tube provided in this field.

This is because when the technical solution of pressurized gas driving the quantitative discharge of liquid is directly combined with the conventional blood collection tube, the pressurized gas input into the liquid floats up to the liquid surface in the form of bubbles, but the process is When the pressure inside the device in the later stage of pressure increases, it is also more and more difficult for the multiple bubbles input by the liquid to float upward, and the floating speed of the bubbles gradually slows down, which prolongs the residence time of the bubbles in the liquid. On the one hand, it not only prolongs the sampling time On the other hand, the increase in the internal pressure of the device will gradually narrow the particle size distribution of the bubbles, prolong the adsorption time between the bubbles and the liquid phase, and increase the gas dissolution rate, which may cause the gas contained in the liquid to dissolve with the liquid. are transported to the outside of the device together, in which case it is difficult to ensure the accuracy of the sampling volume obtained.

Based on the above-mentioned technical problems proposed by the present invention, the present invention provides a hollow plate 6 used in cooperation with the blood collection subpackage assembly 3 for providing gas. The setting of the hollow plate 6 can not only effectively prevent the formation of air The possibility of adsorption, and after the inventors of the present invention practiced and compared a large number of control experimental groups, it proved that the sampling efficiency was effectively improved and the original sampling time could be shortened by more than 45%, and it was proved that the best liquid that can reach 98.5% Accuracy of sample volume.

The structure and installation method of the blood collection subpackage assembly 3 provided by the present invention are described in detail as follows:

Preferably, the blood collection subpackage assembly 3 can controllably deliver the liquid in the blood collection tube body 1 to the blood collection tube body 1 by means of one end of which is fitly connected with the blood collection tube cap 2 of the exterior. Wherein, "controllably" means that the amount of liquid output from the tube body 1 of the blood collection tube is controllable. "The way it can be controlled" can be controlled based on the liquid level information collected by the liquid level monitoring component 8 arranged outside the blood collection tube body 1, or it can be based on the pressurized gas delivered to the inside of the blood collection tube body 1 The relevant parameters are controlled.

The present invention controls the sampling amount by corroborating the above liquid level information and the relevant parameters with each other. , and avoid the inaccuracy of the liquid level information of the liquid mixed with part of the pressurized gas, so the blood collection auxiliary device provided by the present invention not only changes the existing sampling method but also eliminates the blood that may carry infectious viruses in the external environment Contamination problems, but also provides a high-accuracy blood sampling auxiliary method.

Preferably, when the blood collection tube body 1 is in the first placed state, the blood collection subpackage assembly 3 is fitted and connected to the blood collection tube cover 2 in a manner that limits the opening degree of the blood collection tube cover 2, so that One end of the liquid passage 5 and one end of the gas passage 4 on the assembly assembly 3 communicate with the inside of the tube body 1 of the blood collection tube respectively, or when the tube body 1 of the blood collection tube is in a second placement state opposite to the first placement state, the A specified amount of gas enters the inside of the tube body 1 of the blood collection tube through the gas channel 4 and does not form an adsorption effect with the liquid, so as to distribute a specified amount of liquid to the outside of the tube body 1 of the blood collection tube. The "first placement state" refers to the placement state (not shown in the figure) when the blood collection tube cap 2 is positioned above the blood collection tube body 1 . The "second placement state" refers to the placement state when the cap 2 of the blood collection tube is located below the body 1 of the blood collection tube as shown in FIG. 1 . "Limiting the opening degree of the blood collection tube cover 2" means that the area of the open end surface of the blood collection tube cover 2 exposed to the external environment is reduced, that is, it is covered by the blood collection subpackaging assembly 3 . "Compatible connection" refers to detachable connection methods such as threaded connection or snap-fit connection, and rubber gaskets can also be provided between the blood collection sub-package assembly 3 and the blood collection tube cap 2 to further enhance the sealing performance.

The structure and installation method of the hollow plate 6 provided by the present invention which is different from the prior art are described in detail as follows:

First of all, as shown in Figure 2 about the structural schematic view of the hollow plate 6, from left to right, the first schematic diagram is a top view of the hollow plate 6, the second schematic diagram is a bottom view of the hollow plate 6, and the third schematic diagram is the hollow plate in Figure 1. A cross-sectional view of plate 6 along line A-A.

3 is a schematic diagram of a preferred connection between the gas channel 4 and the hollow plate 6 when the gas channel 4 is abutted against the hollow plate 6 and the gas channel 4 communicates with the inner cavity of the hollow plate 6 . Wherein, the solid line arrow refers to the parts on one side of the arrow when the gas passage 4 is abutted on the hollow plate 6 (gas passage 4, first sliding member 11, second sliding member as shown in the figure) 12) The corresponding direction of motion. Wherein, the dotted arrows indicate the flow direction of the liquid inside the gas channel 4 and the liquid inside the second slider 12 when the gas channel 4 is abutted against the hollow plate 6 and the gas channel 4 communicates with the cavity of the hollow plate 6 . Wherein, the arc dotted line between the gas channel 4 and the second sliding member 12 indicates that the two are connected internally.

Preferably, the overall structure of the hollow plate 6 is a cylindrical shape adapted to the shape of the inner wall of the tube body 1 of the blood collection tube. The hollow plate 6 can slide up and down relative to the inner wall of the blood collection tube body 1 under the action of external force. For ease of understanding, here the hollow plate 6 is regarded as a cylinder, and it is stipulated that the hollow plate 6 has a cylinder bottom surface and a cylinder side surface.

Further, for the bottom surface of the hollow plate 6 perpendicular to the penetration direction of the blood collection tube body 1, at least one first through hole 9 is opened in the central area of the bottom surface of the cylinder, and the first through hole 9 It connects the inside of the cylinder and the outside of the cylinder. Therefore, before the actual use of the blood collection device for blood collection, it is necessary to combine blood collection tubes and venous blood collection needles for blood collection. The first through hole 9 opened on the bottom surface is placed inside the blood collection tube body 1; similarly, when the blood collection is completed and the two-way blood collection needle is taken out, the plug end exits the first through hole 9 and the blood collection tube cover 2, and is separated from the blood collection tube cover 2. The inside of the blood vessel body 1, that is, the setting of the hollow plate 6 will not affect the blood collection process.

Further preferably, for the first through hole 9 opened on the bottom surface of the cylinder of the hollow plate 6, the inner wall of the first through hole 9 is provided with a second sliding member 12 and a first sliding member 11, both of which are elastic The ground is slidingly connected on the inner wall of the first through hole 9 . The sliding direction of the first sliding member 11 is parallel to the penetrating direction of the first through hole 9 , and the sliding direction of the second sliding member 12 is perpendicular to the penetrating direction of the first through hole 9 .

Under the condition that no external force is applied to the first sliding member 11, the first sliding member 11 is located on the side end surface of the second sliding member 12 under the action of its corresponding spring in a compressed state, and the second sliding member 12 is at the position of the side surface of the second sliding member 12. The corresponding spring in the compressed state abuts against the outer wall of the side end surface of the second sliding member 12 .

After the gas passage 4 is pierced into the blood collection tube cap 2 and enters the inside of the blood collection tube body 1, the blood collection subpackage assembly 3 is gradually pushed forward, so that the free end of the gas passage 4 abuts against the first sliding member 11, and continues to advance, the free end end pushes the first slider 11 to move until the first slider 11 and the second slider 12 break away from the abutting relationship, and the second slider 12 is released toward a The side moves and abuts against the outer wall of the gas channel 4 , limiting the relatively fixed positional relationship between the gas channel 4 and the hollow plate 6 . Preferably, an elastic rubber pad is provided on the outer edge of the second sliding member 12 close to the open end of the gas channel 4 . The arrangement of the elastic rubber pad is beneficial to improve the relative tightness of the gas passage between the second sliding member 12 and the gas passage 4, and is beneficial to limit the slipping tendency between the two.

Preferably, the side wall of the free end of the gas passage 4 is provided with an opening communicating with the interior thereof, and the second sliding member 12 runs through the front and back, so that the gas passage 4 can transport the gas to the hollow plate through the second sliding member 12 passing through the front and rear 6, the gas enters the gas layer 15 in the tube body 1 of the blood collection tube through the cavity on the bottom surface of the cylinder, the cavity on the side of the cylinder, and the open end on the side of the cylinder in sequence.

More preferably, the outer wall of the blood collection tube body 1 is provided with a scale line for marking the installation position, and the hollow plate 6 is installed on the blood collection tube body 1 in such a way that the position of the first slider 11 is aligned with the scale line internal. Similarly, the blood collection subpackage assembly 3 is also installed on the blood collection tube cover 2 in such a way that the scale lines on its outer wall are aligned with the scale lines on the outside wall of the blood collection tube body 1 . In this way, the gas channel 4 on the blood collection sub-packaging assembly 3 can be aligned with the first sliding member 11 in the hollow plate 6 to complete the relative fixing process between the gas channel 4 and the hollow plate 6 .

A further detailed description of the above-mentioned technical features and principles involved:

After the collected blood sample is centrifuged, the inert separating gel inside the blood collection tube body 1 can completely separate the liquid components (serum or plasma) and solid components (red blood cells, white blood cells, platelets, fibrin, etc.) in the blood, Inert separating gels are intermediate between solid and liquid components based on their physical properties. After standing for a period of time, the release gel solidifies to form a barrier that accumulates on top of the solid components.

At this time, even if the blood collection tube body 1 is inverted, as shown in Figure 1, the inverted blood collection tube body 1, the solid components are blocked by the barrier and cannot move, and can only be kept at the bottom of the blood collection tube body 1 , as shown in Figure 1, the layer at the top of the tube is the solid component layer 13, the layer below the solid component layer 13 is the inert separation glue layer 16, and the bottom of the tube is the liquid component layer 14, which is located at the inert separation layer. The layer between the glue layer 16 and the liquid component layer 14 is the gas layer 15 .

Preferably, when the blood collection tube body 1 is placed vertically in the first placement state, the blood collection subpackage assembly 3 is connected to the blood collection tube cap 2 in a manner that limits the opening degree of the blood collection tube cover 2 . The cover 2 is suitable for sealing connection. One end of the liquid channel 5 and one end of the gas channel 4 located on the blood collection sub-package assembly 3 communicate with the inside of the tube body 1 of the blood collection tube respectively. The first end of the gas channel 4 extending to the inside of the blood collection tube body 1 communicates with the inner cavity of the hollow plate 6 . The first end of the gas channel 4 continues to extend along the inner cavity of the hollow plate 6 .

Preferably, the first end of the gas channel 4 may be flush with the end of the liquid channel 5 on the side where the first end is located. In this case, the hollow plate 6 is arranged at a position close to the cap 2 of the blood collection tube, and the edge of the plate continues to extend along the wall of the tube body. That is, the side of the cylinder of the hollow plate 6 abuts against the wall of the tube body 1 of the blood collection tube and continues to extend upward from the bottom of the cylinder along the tube wall of the tube body.

The liquid channel 5 communicates with the liquid inside the blood collection tube body 1 through the first through hole 9 provided on the hollow plate 6 , and the gas channel 4 communicates with the inner cavity of the hollow plate 6 through the opening provided on the hollow plate 6 .

Preferably, the first end of the gas channel 4 may be higher than the end of the liquid channel 5 on the side where the first end is located. In this case, since the gas channel 4 is relatively long, after the gas channel 4 abuts against the hollow plate 6 , the hollow plate 6 will continue to be pushed to move away from the blood collection tube cover 2 . Existing vacuum blood collection tubes specially used to collect serum samples usually have a blood collection volume of 3ml to 5ml and specifications of 13*75mm or 13*100mm, that is, vacuum blood collection tubes based on existing specifications can use hollow plates of different specifications6 .

Preferably, a pressurized gap 7 for supplying a specified amount of gas to the gas passage 4 is formed when the blood collection subpackage assembly 3 is fitted and connected with the blood collection tube cap 2 . Thus, the gas coming from the outside of the tube body 1 of the blood collection tube first passes through the pressurized gap 7 and then enters the gas channel 4 .

On the one hand, when the gas passage 4 penetrates through the blood collection tube cover 2 and is placed in the liquid inside the blood collection tube body 1, part of the liquid may flow out of the blood collection tube body 1 along the inside of the gas passage 4, and this part of the liquid directly enters the blood collection tube body 1. In the pressure gap 7, it will not flow out and pollute the operating environment;

On the other hand, the setting of the pressurization gap 7 can simply and indirectly detect the air pressure of the gas layer 15 in the tube body 1 of the blood collection tube and buffer the change rate of the air pressure during pressurization or pressure release, avoiding the direct delivery of the gas and effectively controlling it. The problem of the change rate of the air pressure of the gas layer 15 in the tube body 1 of the blood collection tube.

After the gas channel 4 abuts against the hollow plate 6 and the gas channel 4 forms a passage with the inner cavity of the hollow plate 6 , the tube body 1 of the blood collection tube is turned upside down. The first through hole 9 provided on the hollow plate 6 facilitates the flow of the liquid, and further blocks solids above the liquid that may fall off in a pressurized environment. At this time, the liquid and the solid are located at both ends of the blood collection tube body 1 respectively. As shown in FIG. 1 , the open end of the hollow plate 6 is placed in the region where the gas layer 15 above the liquid level is located. Let a specified amount of gas enter the tube body 1 of the blood collection tube sequentially through the gas channel 4 and the cavity of the hollow plate 6 along the pressurization gap 7, and the gas can enter the gas layer 15 inside the tube body without passing through the liquid, That is, there is no adsorption between the gas and the liquid, so that a predetermined amount of liquid can be distributed to the outside of the tube body 1 of the blood collection tube.

Example 2

This embodiment proposes an auxiliary operation device by means of a non-contact blood vessel visualization device 102, and based on the blood collection tube body provided in Embodiment 1, the blood sampling operation performed under the auxiliary operation device is further described in detail, and the implementation The same components as Example 1 will not be repeated in this embodiment.

As shown in FIG. 4 , the auxiliary operation device of the non-contact blood vessel visualization device 102 at least includes a blood collection support pad 101 , a blood vessel visualization device 102 and a central processing module 103 .

As shown in FIG. 5 , the blood collection support pad 101 is used to provide support and fixation for the arm of the blood collection subject. At least one support plane 104 is arranged on the blood collection support pad 101 . The relative spatial position between at least one support plane 104 of the blood collection support pad 101 is determined by medical information. The height parameter in the relative spatial position and the line distance parameter between the two support planes 104 can be adjusted based on people of different heights.

The blood vessel visualization device 102 is assembled above the blood collection support pad 101 . The blood vessel visualization device 102 is used to cooperate with the blood collection support pad 101 to provide hemodynamic monitoring for projecting the subsurface structure onto the object surface. The blood collection support pad 101 is installed on the platform plane below the blood vessel visualization device 102 . The blood collection support pad 101 can move back and forth relative to the plane of the platform. In actual use, the blood vessel visualization device 102 is installed on the top of the arched blood sampling window. Hemodynamic monitoring includes at least the monitoring of blood flow. Blood flow refers to the volume of blood passing through a certain section of a blood vessel per unit time, also known as volume velocity. In fact, this is the blood flow rate, which depends on the pressure difference between the two ends of the blood vessel and the resistance of the blood vessel to blood flow. The measurement of blood flow velocity has practical value for checking cardiovascular function and diagnosing vascular diseases. Slow blood flow indicates that the blood pressure is not high enough, or the vascular resistance is too high. There are many traditional blood flow measurement methods, such as ultrasonic Doppler method, laser Doppler method, nuclear magnetic resonance method and so on. The blood vessel visualization device 102 utilizes that when infrared rays irradiate human tissues, the dehemoglobin in the veins has a more obvious absorption effect on infrared rays than the surrounding tissues, and will form a very obvious image of veins and blood vessels when captured by an infrared camera lens. After being converted by a special software and hardware image processing system, visible light is projected back to the skin surface to form a visible image of veins and blood vessels, which assists in the positioning and display of veins. Based on the above principles, the blood vessel visualization device 102 realizes the display of subcutaneous veins that are difficult to see clearly, and is used for auxiliary positioning and display of superficial veins on the back of the hand, the back of the foot, the elbow and wrist, and the scalp. Through the blood vessel visualization device 102, the accuracy of venous blood vessel positioning and the success rate of venipuncture can be improved, and the pain of the subject to be blood collected and the conflict between doctors and patients can be reduced. Although the blood vessel visualization device 102 is currently capable of observing the direction of blood flow and qualitatively evaluating the blood flow rate, there is no auxiliary device for quantitatively evaluating the blood flow through the blood vessel visualization device 102 . In the actual venous blood collection process assisted by the vascular visualization device 102, the medical personnel usually judge whether the selected blood vessel meets the puncture conditions, especially in the process of tumor drug injection. In the event of tissue damage or mechanical damage, it is necessary to press one end of the blood vessel in the mapping area of the blood vessel visualization device 102 and then release it. Under this operation, the flow of the blood vessel is first cut off at one end and then resumed, so as to achieve qualitative assessment. However, the auxiliary operating device provided by the present invention realizes the quantitative evaluation of blood flow by using this simple operation. The quantitative evaluation process is performed by combining the high-definition imaging features of the blood vessel visualization device 102 with this simple operation to perform hemodynamic analysis. A method for quantitatively assessing its blood flow is provided. The individualized quantitative assessment of blood flow is to provide assistance for the blood collection operation of medical staff, especially for unskilled medical staff who cannot know the timing of loosening the cuff, or especially for unskilled unskilled medical staff who cannot Provide assistance to medical staff who know when to stop blood collection.

According to a preferred embodiment, the central processing module 103 performs information interaction with the hospital medical system to determine at least Based on the blood collection image analysis of the image information collected by the blood vessel visualization device 102 that provides hemodynamic monitoring, it assists the medical staff to complete the blood collection operation.

According to a preferred implementation manner, the blood collection image analysis is based on the image information collected by the blood vessel visualization device 102 . The blood vessel visualization device 102 is provided with at least two cameras, one of which is configured as an infrared camera lens for collecting reflected infrared light intensity. At least one camera installed on the blood vessel visualization device 102 is used to monitor the needle puncture removal operation. Preferably, the central processing module 103 is capable of judging needle puncture removal based on the image information collected by the camera. Preferably, the central processing module 103's determination of needle puncture removal includes at least one or more of the following steps:

S1: Receive the video collected by the camera at the first moment and determine the pre-blood collection frame, based on the relative positional relationship between at least one support plane 104 on the blood collection support pad 101 and the blood vessel visualization device 102, determine in the pre-blood collection frame At least including the blood collection area frame of the blood collection site of the subject to be blood collection;

S2: continue to extract the frame number of the video and perform target recognition and detection on the extracted frame number, and at the second moment, determine at least one needle area frame including at least the needle part in the keras environment based on the target detection method of the YOLOv3 algorithm;

S3: Determine whether the blood collection area frame R1 overlaps with the needle area frame R2. When the intersection ratio between the two is greater than the overlap rate obtained by the union between the two, the overlap rate is R1/(R1+R2). The area size of the needle area frame is detected;

S4: At the third moment, the target detection method based on the YOLOv3 algorithm in the keras environment determines the needle area frame whose current area is not greater than 1/2 of the original area, and obtains the puncture operation information of the blood collection needle and the instruction information used to indicate the cuff 106 action ;

S5: At the fourth moment, the target detection method based on the YOLOv3 algorithm in the keras environment determines the needle area frame whose current area is not smaller than the original area, and obtains the operation information of removing the blood collection needle and the command information for instructing the action of the airbag assembly 105 .

According to a preferred implementation manner, the central processing module 103 performs information interaction with the hospital medical system. Preferably, the hospital medical system includes at least a priority list of blood collection subjects in the blood collection department and medical information corresponding to the next blood collection subject. The medical information includes at least the height, weight, age, medical history and other information of the subject to be blood collected. The central processing module 103 assists in completing the blood collection operation based on the blood collection image analysis of the blood vessel visualization device 102 by controlling the blood collection support pad 101 to cooperate with the blood vessel visualization device 102 in the case of determining the medical information of the next blood collection subject . The process of using the blood collection support pad 101 in cooperation with the blood vessel visualization device 102 at least includes that the central processing module 103 adjusts the relative spatial position between at least one support plane 104 of the blood collection support pad 101 based on the medical information to determine the position in the arm of the blood collection object. When the blood vessel visualization device 102 is placed on the support plane 104 in such a manner as to expose the part to be blood-collected, it can collect at least the first image information about the part to be blood-collected of the subject to be blood-collected and the second image collected when the medical staff performs a trigger event information. At least one support plane 104 is provided on the blood collection support pad 101 . The support plane 104 is used to place the elbow of the subject to be blood-collected or the wrist of the subject to be blood-collected. The relative spatial position between at least one support plane 104 is the vertical or horizontal direction or the support plane 104 for supporting the elbow of the subject to be blood-collected and the support plane 104 used to support the wrist of the subject to be blood-collected. The relative positions formed relative to each other on the respective defined planes. The central processing module 103 regulates the relative spatial position between at least one support plane 104 of the blood collection support pad 101 based on the medical information. According to the excerpts of "Human Dimensions of Chinese Adults GB10000-88" and "Human Dimensions of Working Space GB/T 13547-92", it can be known that the arm lengths of people of different heights, genders and ages are significantly different. However, in the prior art, either the blood collection method without using the blood collection support pad 101 or the blood collection method with a fixed-shaped blood collection support pad 101 is not conducive to individual needs, and the blood collection experience provided to the blood collection subjects is poor and individual differences are serious. Affected the blood collection work efficiency of medical personnel. However, the auxiliary operating device provided by the present invention adopts a more flexible split-type blood collection support pad 101, and the blood collection support pad 101 can be adjusted before the next blood collection object is performed by using the existing medical information of the hospital medical system. To a large extent, it is suitable for the corresponding supporting state of the medical information, and the supporting plane 104 with a certain surface area can provide a further accurate position for placing the arm of the subject to be blood-collected, so as to meet the different individual needs of different subjects for blood-collecting to the greatest extent and at the same time facilitate Improve the efficiency of blood collection for medical staff.

Preferably, the central processing module 103 can be based on at least the hemodynamic information and The time correlation feature between the at least one blood collection phase assists in completing at least one blood collection phase in the process from before blood collection to the end of hemostatic compression. Preferably, the first image information refers to the width and dimension information of at least one blood vessel located in its mapping area, which is collected by the blood vessel visualization device 102 and transmitted to the central processing module 103 for blood collection image analysis and processing. The central processing module 103 analyzes and processes the first image information to obtain width dimension information and cross-sectional information. The mapping area refers to the monitoring area of the blood vessel visualization device 102 about the blood sampling site of the blood sampling object. Preferably, the second image information collected by the blood vessel visualization device 102 is collected at a first duration or a first moment when a trigger condition preset by the central processing module 103 is satisfied. The central processing module 103 pre-stores trigger conditions, and the trigger conditions at least include that the blood vessels are in on, off, and on states successively within a predetermined time period. The executor of the trigger event corresponding to the trigger condition is the medical staff who performs the blood collection operation for the blood collection object. The activation of the trigger condition is based on the manual operation that the medical staff presses one end of the blood vessel in the mapping area of the blood vessel visualization device 102 and then quickly releases it. Under manual operation, the blood vessel is first converted from the original unobstructed state to the state of cut-off flow at both ends, and then returns to the state of smooth flow. The second image information at least includes the relative distance between the two ends of the broken flow and the time for the two ends of the broken flow to recover unimpeded. The central processing module 103 can process the obtained average linear velocity of the blood flow in the blood vessel segment based on the second image information. The average linear velocity V of the venous blood flow is proportional to the blood flow Q and inversely proportional to the total area of the blood vessel cross-section S, blood flow Q=V*S. The blood sampling image analysis at least includes the central processing module 103 receiving and analyzing the first image information and the second image information to obtain hemodynamic information including at least the blood flow quantitative evaluation results of the blood sampling subject.

Preferably, the central processing module 103 assists in completing at least one blood collection phase in the process from before blood collection to the end of hemostatic compression based on the time correlation feature between hemodynamic information and at least one blood collection phase. Further preferably, the central processing module 103 divides the blood collection operation into at least one blood collection phase with a time sequence in advance. The blood collection stage at least includes the blood collection preparation stage of placing the arm of the blood collection subject on the blood collection support pad 101 and selecting a suitable vein for blood collection with the assistance of the blood vessel visualization device 102 , and performing puncture with the assistance of the blood vessel visualization device 102 until the end of the puncture The final blood collection stage, the blood collection and hemostasis stage after the blood collection object presses the arm to stop the bleeding until the end of the bleeding. Preferably, the temporal correlation feature refers to obtaining the predicted collected blood volume associated with different blood collection stages in different blood collection stages at different times of the blood collection process based on blood collection image analysis including at least hemodynamic analysis.

According to a preferred embodiment, the blood collection support pad 101 is provided with a cuff 106 for compressing the vein before blood collection to make the vein appear filled with blood. Through the cuff 106 that acquires effective sensing information when at least one sensing component collects a trigger event, the effective sensing information acquired by the sensing component when the cuff 106 is in a closed loop and the sensing component is relatively fixed to the support plane 104 is sent To the central processing module 103 that obtains pressure information under the condition that at least one pressure sensor on the cuff 106 is in contact with the skin of the subject to be blooded, so that the information of the subject to be drawn blood can be acquired through information acquisition with the hospital medical system The central processing module 103 can instruct at least one expandable component on the inner wall of the closed loop cuff 106 and provided with at least one pressure sensor to expand when the effective sensing information is obtained, and when the pressure information reaches the preset When the threshold is pressed, the expandable assembly is instructed to maintain the current expanded position. The central processing module 103 that monitors the operation of inserting and pulling out the blood collection needle by analyzing the blood collection image information collected by the blood vessel visualization device 102 transmits the instruction information generated when the blood collection needle is punctured by analyzing the blood collection image to the At least one inflatable component makes the pressure information of the pressure sensor in contact with the skin of the subject to be blood-collected meet the cuff 106 with a preset pressure threshold, so that the inflatable component on the cuff 106 that maintains the current expanded posture is according to the central processing module Step 103 is to perform a pressure relief operation based on the instruction information generated when the blood collection needle is punctured, and return to the initial posture in which the pressure information collected by the pressure sensor does not reach the preset pressure threshold.

According to a preferred embodiment, the auxiliary operation device further includes an air bag assembly 105 for the blood sampling subject to hold in a non-empty fist. The air bag assembly 105 is arranged on at least one support plane 104 of the blood collection support pad 101 and it can respond to the instruction information sent by the central processing module 103 to introduce gas from the outside of the air bag assembly 105 for expansion, so as to prompt in time to wait The timing at which the blood sampling subject releases its fist. The central processing module 103 that monitors the operation of inserting and pulling out the blood collection needle by performing blood collection image analysis on the image information collected by the blood vessel visualization device 102 transmits the instruction information generated when it obtains the operation of removing the blood collection needle by analyzing the blood collection image to the An external gas pressurizing device is used to control the air bag assembly 105 in the expansion and contraction state, so that the air bag assembly 105 held in the hand of the blood collection subject in its expanded state during the blood collection operation is determined according to the central processing module 103 when the blood collection needle is pulled out. The generated instruction information is pressurized by its external gas pressurizing device to prompt and/or force the subject to be blood-collected to be held outside the airbag assembly 105 while maintaining a clenched fist posture in its gradually inflated manner during the blood-collecting operation The hands are switched to the deployment posture that no longer holds the airbag module 105 . Therefore, it is possible to cancel the oppressive effect of the cuff 106 on the hand of the blood collection subject immediately after the medical staff withdraws from the blood collection needle without manual operation by the medical staff.

According to a preferred embodiment, the central processing module 103 is also configured to: when the medical information of the next blood collection subject is acquired through the hospital medical system, transmit the instruction information about the palm size parameter corresponding to the medical information to The airbag assembly 105 in an inflated state to force the hand of the previous blood collection subject to take an unfolded posture, so that the airbag assembly 105 circumscribes through it when it is out of the grip relationship with the hand of the previous blood collection subject in the unfolded posture. The gas pressurizing device performs a pressure release operation to convert it into an inflated state suitable for the medical information of the next blood sampling subject. Therefore, the pressure release operation of the air bag assembly 105 provides convenience for the hand of the previous blood collection subject to detach from the blood collection support pad 101 and at the same time provides an expanded state suitable for the next blood collection subject to hold.

According to a preferred embodiment, the blood collection support pad 101 includes at least one support plane 104 that is convenient for exposing the site to be blood collection and has relative spatial positions with each other. The central processing module 103 acquires the medical information of the next blood sampling subject through the hospital medical system, and transmits the instruction information about the arm span size parameter corresponding to the medical information to the previous blood sampling subject through at least one supporting plane 104. The blood collection support pad 101 of the first relative spatial position adapted to the arm size parameters, so that the blood collection support pad 101 passes through its at least one support plane 104 respectively relative to the imaginary plane used to support the blood collection support pad 101 by at least one The horizontal movement defined by the arrangement direction of the support plane 104 or the vertical direction perpendicular to the horizontal direction is converted into a second relative spatial position suitable for the medical information of the next blood collection subject, so that the blood collection support The pad 101 only needs to make small or no adjustments to the support plane 104 in the existing relative spatial position to provide a relative spatial position suitable for the next blood sampling subject to place the arm.

According to a preferred implementation manner, the central processing module 103 is configured to obtain the first image information collected by the blood vessel visualization device 102 at least about the blood collection site of the subject to be collected and/or the first image information collected when the medical staff executes the trigger event. The second image information is obtained by performing blood collection image analysis on the first image information and/or the second image information, and the blood vessel visualization device 102 that transmits real-time image information to the hand of the subject to be blood collection provides medical personnel with information about the blood collection. The hemodynamic information of the prompt information of sufficient blood volume.

The auxiliary operation method by means of the auxiliary operation device of the non-contact blood vessel visualization device 102, the auxiliary operation method at least includes the blood collection support pad 101, the blood vessel visualization device 102 and the central processing module 103, and the blood collection support pad 101, which is used for The arm of the blood collection subject provides support and fixation. The blood vessel visualization device 102 is assembled above the blood collection support pad 101 and is used to cooperate with the blood collection support pad 101 to provide hemodynamics for projecting the subsurface structure onto the object surface. The monitoring and auxiliary operation method at least includes the following steps: the central processing module 103 performs information interaction with the hospital medical system, and controls the blood collection support pad 101 to cooperate with the blood vessel visualization device 102 in the case of determining the medical information of the next blood collection object The method is at least based on the blood collection image analysis of the image information collected by the blood vessel visualization device 102 that provides hemodynamic monitoring to assist the medical staff to complete the blood collection operation.

Specifically, in the blood collection preparation stage, based on the blood collection support pad 101 that has been roughly adjusted automatically, the arm of the subject to be blood sampling is completely exposed at the expected angle to the part to be blood sampling, and the medical staff instructs the subject to hold the blood collection lightly and in a certain swelling state. With the airbag assembly 105, the object to be collected is no longer the traditional empty fist posture that is not conducive to the perception of the holding strength of the object to be collected during blood collection, but is effectively maintained under the airbag assembly 105 in a relatively constant holding strength during the blood collection process. At the same time, the medical personnel wrap the body of the cuff 106 with the induction component around the hand of the blood collection subject and fix it on the blood collection support pad 101. Based on the effective sensing information of the induction component, the cuff 106 is externally connected to the gas pressurization device Press down on the arm of the subject to be blood-collected until the predetermined pressure threshold is reached. This pressurizing operation is the indication information provided by the central processing module 103 after processing the information of the subject to be blood-collected and determining whether the subject to be blood-collected has historical medical information about weak blood vessel walls or is an elderly person with excessively old blood vessel wall elasticity deviation ongoing. In this way, when it is detected that the subject to be blood sampled has historical medical information about weak vessel walls or is an elderly person with excessively aged vessel wall elasticity deviations, the pressure threshold can be reduced accordingly to avoid the problem of vessel wall rupture. At this time, the blood vessel visualization device 102 projects the video information acquired by its infrared camera lens to the blood collection site, and the medical staff selects a blood vessel suitable for puncturing by simply pressing and releasing both ends of the blood vessel. Preferably, the blood vessel visualization device 102 supports medical personnel to narrow the projection area to a smaller area of the blood vessel to be punctured. After sterilizing the blood collection site, the medical staff will puncture the needle of the blood collection needle into the blood collection site. At this time, the central processing module 103 can then instruct the cuff 106 to remove the pressure on the arm of the blood collection subject based on the processed blood collection and puncture operation information. . Preferably, when the central processing module 103 detects that the cuff 106 has been tied for more than 1 minute, it prompts medical staff or instructs the cuff 106 to perform a pressure release operation. When cuff 106 is banded for more than 1 minute, the blockage of blood flow in large veins will increase the internal pressure of capillaries, make blood in blood vessels communicate with tissue fluid, and allow substances with a relative molecular weight of less than 5000 to escape into the tissue fluid. If the time is prolonged, hypoxia in local tissues will cause large changes in blood components, and the test results will increase or decrease unduely. During the blood collection process, the central processing module 103 performs information interaction with the hospital medical system to obtain the blood collection information of the next blood collection object including at least the blood collection volume. When the blood collection and puncture operation information is determined, the central processing module 103 obtains The blood flow information and blood collection information are used to estimate the blood collection time, so as to remind the medical staff when the estimated blood collection time is reached. The medical staff then covered the needle hole with a sterile cotton ball and pulled the needle out of the vein. At this time, the central processing module 103 can then instruct the air bag assembly 105 to perform a pressurizing operation based on the obtained blood collection and removal operation information to prompt the blood collection subject to release his fist. In this way, it is possible to reduce the situation where hard fists make the blood vessel wall difficult to repair and cause blood to flow out of the blood vessel and cause blood stasis. The auxiliary operation device provided by the invention is suitable for common routine blood collection operations. The auxiliary operating device provided by the present invention is also suitable for blood donation and blood collection operations that need to collect at least 200ml, especially when a single medical staff collects blood for two blood donors at the same time and their attention is inevitably distracted, it can effectively and reliably prompt the medical staff processing sequence. The auxiliary operating device provided by the present invention is also suitable for hemodialysis operations with a large amount of blood exchange, especially for the occurrence of cardiovascular complications due to the large difference between the blood sampling speed and the backflow speed, it can effectively and reliably prompt the medical staff.

It should be noted that the above specific embodiments are exemplary, and those skilled in the art can come up with various solutions inspired by the disclosure of the present invention, and these solutions also belong to the scope of the disclosure of the present invention and fall within the scope of this disclosure. within the scope of protection of the invention. Those skilled in the art should understand that the description and drawings of the present invention are illustrative rather than limiting to the claims. The protection scope of the present invention is defined by the claims and their equivalents.

Claims (5)

1. Blood collection aids for separating serum samples from whole blood, comprising at least: Blood collection tube body (1), used for storing the collected liquid; A blood collection tube cover (2), which is assembled on the blood collection tube body (1); and A blood collection subpackage assembly (3), capable of controllably delivering the liquid in the blood collection tube body (1) to the blood collection tube body (1) by means of one end of which is fitted and connected to the blood collection tube cap (2). the outside of the vessel body (1), It is characterized by, The blood collection dispensing assembly (3) is configured to make the storage The liquid in the blood collection tube body (1) is output in batches with a specified amount, Wherein, the blood collection tube body (1) is provided with a gas channel (4) that allows the blood collection subpackage assembly (3) to communicate with the inner cavity of the blood collection tube cover (2) when it is fitted and connected. The hollow plate (6), the hollow plate (6) is assembled to the blood collection tube body (1) before the blood collection tube body (1) is used, wherein, The two ends of at least one first through hole (9) provided on the plate body of the hollow plate (6) communicate with the inside of the blood collection tube body (1) respectively and communicate with the inside of the hollow plate (6). The lumen is isolated. 2. The blood collection auxiliary device according to claim 1, characterized in that, The blood collection auxiliary device at least includes a pressurized gap (7), which is formed when the blood collection subpackage assembly (3) is fitted and connected with the blood collection tube cover (2) An area for supplying a prescribed amount of gas to the gas channel (4). 3. The blood collection auxiliary device according to claim 1, characterized in that, The blood collection auxiliary device includes at least a liquid level monitoring component (8), wherein the blood collection auxiliary device is configured to: Based on the specified amount of gas input along the gas channel (4) and the liquid flow rate between different liquid level positions monitored by the liquid level monitoring component (8) at different times, it is determined to be allocated to the blood collection tube Whether the liquid flow outside the body (1) meets the specified amount. 4. The blood collection auxiliary device according to claim 1, characterized in that, The blood collection aid is configured to: Obtaining the third liquid collected by the blood collection auxiliary device when the blood collection tube body (1) is in the second placement state where the blood collection tube cap (2) is located below the blood collection tube body (1) The surface position and the fourth liquid level position are used to determine the total amount of liquid collected by the tube body (1) of the blood collection tube so that the user can determine at least one distribution method of the liquid based on the total amount of liquid. 5. The blood collection auxiliary device according to claim 1, characterized in that, Part of the plate surface on the hollow plate (6) extends away from the blood collection tube cover (2), so that the inner cavity of the hollow plate (6) has a An open end extending in the direction of the central axis of the hollow plate (6) and located on a different imaginary horizontal plane from the plate surface of the hollow plate (6).

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