CN111254053B - Hydrogen peroxide instantaneous sterilization device and sterilization method - Google Patents
Hydrogen peroxide instantaneous sterilization device and sterilization method Download PDFInfo
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Abstract
The invention discloses a hydrogen peroxide instantaneous sterilization device and a sterilization method, and aims to solve the defect of low sterilization speed of the sterilization device. The invention comprises a compressed air filter, a vaporization cylinder, a sterilization pipe and a hydrogen peroxide liquid storage bottle, wherein a heating rod and an evaporation disc are arranged in the vaporization cylinder, a liquid conveying pipe is communicated between the hydrogen peroxide liquid storage bottle and the vaporization cylinder, an infusion pump is arranged on the liquid conveying pipe, an air inlet pipe is communicated between the compressed air filter and the vaporization cylinder, an air inlet valve and a pressure regulating valve are arranged on the air inlet pipe, a sterilization air conveying pipe is communicated between the sterilization pipe and the vaporization cylinder, a sterilization air conveying valve is arranged on the sterilization air conveying pipe, an exhaust pipe is communicated with the sterilization pipe, an exhaust valve is arranged on the exhaust pipe, a concentration detection pipe is communicated between the exhaust pipe and the sterilization air conveying pipe, a concentration valve and a hydrogen peroxide concentration sensor are arranged on the concentration detection pipe, a residual exhaust pipe is communicated between the air inlet pipe and the sterilization air conveying pipe, and a residual exhaust valve is arranged on the residual exhaust pipe.
Description
Technical Field
The invention relates to a sterilization device, in particular to a hydrogen peroxide instantaneous sterilization device and a sterilization method.
Background
With the recent rise of CAR-T cell immunotherapy, there is a demand for corresponding immunotherapeutic cell preparation equipment. A typical CAR-T treatment procedure includes five steps: isolation, modification (CAR-T cell preparation), expansion, reinfusion and monitoring. In order to increase the efficiency of cell preparation and the level of automation, cell therapy workstations (cell production isolators) have been developed. Wherein the operations of cell separation, modification and the like are completed in a cell production isolator. When the blastocyst tube and the cryopreservation tube (for short, cell raw materials) with cells enter the cell isolator, sterile transmission needs to be realized, and a special sterilizer and a transmission channel need to be developed. The process requirement of cell production is that the quick sterilization and quick transmission of cell raw materials are realized, corresponding sterilization equipment is needed, the sterilization speed of a traditional sterilizer is low, the sterilization period is generally 3-5 hours, the sterilization period is the shortest and is more than 30 minutes, and the quick sterilization requirement cannot be met.
Disclosure of Invention
The invention overcomes the defect of low sterilization speed of the existing sterilization device, and provides the hydrogen peroxide instantaneous sterilization device and the sterilization method, which have the advantages of high sterilization speed and good sterilization effect.
In order to solve the technical problems, the invention adopts the following technical scheme: the utility model provides a hydrogen peroxide instantaneous sterilization device, including compressed air filter, the vaporization section of thick bamboo, sterilization pipe, hydrogen peroxide stock solution bottle, install heating rod and evaporating dish in the vaporization section of thick bamboo, communicate the liquid pipe between hydrogen peroxide stock solution bottle and the vaporization section of thick bamboo, install the infusion pump on the liquid pipe, communicate the intake pipe between compressed air filter and the vaporization section of thick bamboo, install air inlet valve and air-vent valve on the intake pipe, communicate the sterilization air pipe between sterilization pipe and the vaporization section of thick bamboo, install the sterilization air-vent valve on the sterilization pipe, install the discharge valve on the discharge pipe, communicate concentration detection pipe between blast pipe and the sterilization air pipe, install concentration valve and hydrogen peroxide concentration sensor on the concentration detection pipe, communicate between intake pipe and the sterilization air pipe and arrange the incomplete pipe, arrange the incomplete valve on the incomplete pipe.
The sterilization operation comprises the following steps: a. the method comprises the steps of installing a sterilization pipe on an isolator, arranging an outlet end of the sterilization pipe in the isolator, arranging an inlet end of the sterilization pipe outside the isolator, and arranging a sterilization object into the sterilization pipe from the outer end of the sterilization pipe; b. in the dehumidification stage, an air inlet valve, a pressure regulating valve, a sterilization air supply valve, an air outlet valve and a concentration valve are opened, a heating rod and an evaporation tray work and are preheated, and the inside of a sterilization tube is dehumidified to below 10% RH; when the temperature in the sterilizing tube reaches 37 ℃, all valves are closed, meanwhile, after the temperature on the evaporating pan reaches 140 ℃, hydrogen peroxide drops are injected through a delivery pump, an air inlet valve and a concentration valve are opened, a concentration adjusting stage is carried out, dry compressed air in the process enters a vaporizing cylinder through a compressed air filter and a pressure regulating valve, a pipeline is preheated through hot air in the vaporizing cylinder, and hydrogen peroxide gas is prevented from being contacted with a tube wall to be condensed into liquid hydrogen peroxide in the sterilizing process; c. the method comprises the steps of adjusting, setting the injection amount of hydrogen peroxide, starting a delivery pump to inject a hydrogen peroxide solution into a vaporization cylinder to vaporize the hydrogen peroxide solution to generate gaseous hydrogen peroxide, mixing the vaporized hydrogen peroxide gas with air, detecting the concentration by a hydrogen peroxide concentration sensor, discharging the mixture into an exhaust pipe, detecting the hydrogen peroxide concentration in real time by the hydrogen peroxide concentration sensor, and closing the concentration valve to enter a sterilization stage when the concentration value reaches 3000 ppm; d. in the sterilization stage, a sterilization air supply valve and an exhaust valve are opened, hydrogen peroxide gas is introduced into the sterilization tube, and the sterilization action time is 20 to 60 seconds; e. residue removal stage: closing a sterilization air supply valve, opening a residual discharge valve, an air inlet valve, a pressure regulating valve, a concentration valve and an exhaust valve, discharging residual hydrogen peroxide gas in a sterilization pipe and a vaporization cylinder by dry compressed air sent by a compressed air filter, closing all valves when the hydrogen peroxide concentration is less than 1ppm, discharging the residual, and taking out the sterilized materials from an isolator through an outlet end of the sterilization pipe. The whole sterilization process is fast and the sterilization effect is good.
Preferably, the liquid feeding pipe is provided with a one-way valve. The arrangement of the one-way valve prevents the hydrogen peroxide liquid from flowing back.
Preferably, a temperature sensor is installed in the vaporization cylinder. The temperature sensor is convenient for controlling the temperature.
Preferably, a sterilization temperature sensor and a hydrogen peroxide sterilization concentration sensor are installed in the sterilization tube. This arrangement facilitates control of the temperature and hydrogen peroxide gas concentration in the sterilization tube, ensuring the sterilization effect.
Preferably, both ends of the sterilizing tube are respectively connected with an inlet end cover and an outlet end cover, and a flange is arranged on the side, close to the outlet end cover, of the sterilizing tube. The flange facilitates the connection of the sterilization tube and the isolator. The inlet end cover and the outlet end cover ensure the sealing of the two ends of the sterilizing tube.
Preferably, the sterilizing tube is provided with an induction switch close to the inlet end cover, and the inlet end cover is provided with an induction head corresponding to the induction switch. The arrangement of the inductive switch and the inductive head is convenient for judging whether the inlet end cover is connected in place.
Preferably, the device also comprises a mounting box, wherein a compressed air filter, a vaporization cylinder, a hydrogen peroxide liquid storage bottle, an infusion pump, an air inlet valve, a pressure regulating valve, a sterilization air supply valve, an exhaust valve, a concentration valve and a residual discharge valve are all arranged in the mounting box. The parts are arranged in the mounting box, the structure is compact, and the device is convenient to use.
Preferably, an air inlet and an air outlet are arranged on the sterilizing tube, a protective sleeve is arranged at the position of the air inlet in the sterilizing tube, a rotating shaft is arranged in the protective sleeve, a plurality of driving blades are uniformly arranged at one end of the rotating shaft, and a rotating disc is arranged at the other end of the rotating shaft; a material carrying plate and a sliding bar are arranged in the sterilizing tube, a sliding block is arranged on the lower surface of the sliding bar, a sliding rail is arranged on the inner wall of the sterilizing tube, the sliding block is slidably arranged on the sliding rail, a plurality of pushing protrusions are arranged on the upper surface of the sliding bar at intervals, the pushing protrusions are of a V-shaped structure, a plurality of pushing rods are inserted on the material carrying plate at intervals, a pushing rod is arranged between every two adjacent pushing protrusions, the upper ends of the pushing rods are connected with a supporting bar, and the supporting bar is supported on the material carrying plate; and a connecting rod is hinged between the end part of the sliding strip and the edge of the turntable.
The sterilizing object is placed on the object carrying plate in the sterilizing tube, the hydrogen peroxide gas fed in from the air inlet drives the driving blade to rotate, so that the rotation of the rotating shaft is realized, the rotating disc rotates along with the rotating shaft, the rotating disc drives the sliding bar to reciprocate through the connecting rod, the pushing protrusion on the sliding bar slides over the lower end of the pushing rod, the pushing rod is lifted upwards, the sterilizing object contacted with the supporting bar is lifted upwards and even turned, and the lower surface of the sterilizing object is guaranteed to be in direct contact with the hydrogen peroxide gas, so that the sterilizing object can be rapidly sterilized. In the whole sterilization process, the sterilization objects are in a dynamic state, which is beneficial to improving the sterilization speed and the sterilization effect.
A sterilization method of a hydrogen peroxide transient sterilization device, comprising the steps of: a. the method comprises the steps of installing a sterilization pipe on an isolator, arranging an outlet end of the sterilization pipe in the isolator, arranging an inlet end of the sterilization pipe outside the isolator, and arranging a sterilization object into the sterilization pipe from the outer end of the sterilization pipe; b. in the dehumidification stage, an air inlet valve, a pressure regulating valve, a sterilization air supply valve, an air outlet valve and a concentration valve are opened, a heating rod and an evaporation tray work and are preheated, and the inside of a sterilization tube is dehumidified to below 10% RH; when the temperature in the sterilizing tube reaches 37 ℃, all valves are closed, meanwhile, after the temperature on the evaporating pan reaches 140 ℃, hydrogen peroxide drops are injected through a delivery pump, an air inlet valve and a concentration valve are opened, a concentration adjusting stage is carried out, dry compressed air in the process enters a vaporizing cylinder through a compressed air filter and a pressure regulating valve, a pipeline is preheated through hot air in the vaporizing cylinder, and hydrogen peroxide gas is prevented from being contacted with a tube wall to be condensed into liquid hydrogen peroxide in the sterilizing process; c. the method comprises the steps of adjusting, setting the injection amount of hydrogen peroxide, starting a delivery pump to inject a hydrogen peroxide solution into a vaporization cylinder to vaporize the hydrogen peroxide solution to generate gaseous hydrogen peroxide, mixing the vaporized hydrogen peroxide gas with air, detecting the concentration by a hydrogen peroxide concentration sensor, discharging the mixture into an exhaust pipe, detecting the hydrogen peroxide concentration in real time by the hydrogen peroxide concentration sensor, and closing the concentration valve to enter a sterilization stage when the concentration value reaches 3000 ppm; d. in the sterilization stage, a sterilization air supply valve and an exhaust valve are opened, hydrogen peroxide gas is introduced into the sterilization tube, and the sterilization action time is 20 to 60 seconds; e. residue removal stage: closing a sterilization air supply valve, opening a residual discharge valve, an air inlet valve, a pressure regulating valve, a concentration valve and an exhaust valve, discharging residual hydrogen peroxide gas in a sterilization pipe and a vaporization cylinder by dry compressed air sent by a compressed air filter, closing all valves when the hydrogen peroxide concentration is less than 1ppm, discharging the residual, and taking out the sterilized materials from an isolator through an outlet end of the sterilization pipe.
Compared with the prior art, the invention has the beneficial effects that: when the hydrogen peroxide instantaneous sterilization device performs sterilization operation, the sterilization speed is high, and the sterilization effect is good.
Drawings
FIG. 1 is a schematic diagram of the present invention;
FIG. 2 is a schematic view of the structure of the present invention in use;
FIG. 3 is a schematic view of a partial structure of the present invention;
FIG. 4 is a schematic view of the structure of the sterilizing duct of the present invention;
FIG. 5 is a schematic view showing the structure of a sterilizing duct according to embodiment 2 of the present invention;
in the figure: 1. the device comprises a mounting box, 2, a compressed air filter, 3, a vaporization cylinder, 4, a sterilizing pipe, 5, a hydrogen peroxide liquid storage bottle, 6, a heating rod, 7, an evaporation tray, 8, a liquid delivery pipe, 9, an infusion pump, 10, an air inlet pipe, 11, an air inlet valve, 12, a pressure regulating valve, 13, a sterilizing air delivery pipe, 14, a sterilizing air delivery valve, 15, an exhaust pipe, 16, an exhaust valve, 17, a concentration detection pipe, 18, a concentration valve, 19, a hydrogen peroxide concentration sensor, 20, a residue discharge pipe, 21, a residue discharge valve, 22, a flowmeter, 23, a check valve, 24, a temperature sensor, 25, a sterilizing temperature sensor, 26, a hydrogen peroxide sterilizing concentration sensor, 27, an inlet end cover, 28, an outlet end cover, 29, a flange, 30, a sensing switch, 31, a sensing head, 32, an air inlet, 33, an air outlet, 34, a protective sleeve, 35, a rotating shaft, 36, a driving blade, 37, a rotating disc, 38, a carrier plate, 39, a sliding bar, 40, a sliding block, 41, a sliding rail, 42, a pushing protrusion, 43, a supporting bar, 44, a 45, a flange, 45, a connecting rod, a 46 and a limit lever.
Detailed Description
The technical scheme of the invention is further specifically described by the following specific embodiments with reference to the accompanying drawings:
example 1: a hydrogen peroxide instantaneous sterilization device (see fig. 1-4) comprises a mounting box 1, a compressed air filter 2, a vaporization cylinder 3, a sterilization pipe 4 and a hydrogen peroxide liquid storage bottle 5, wherein a heating rod 6 and a vaporization disk 7 are arranged in the vaporization cylinder, a liquid conveying pipe 8 is communicated between the hydrogen peroxide liquid storage bottle and the vaporization cylinder, an infusion pump 9 is arranged on the liquid conveying pipe, an air inlet pipe 10 is communicated between the compressed air filter and the vaporization cylinder, an air inlet valve 11 and a pressure regulating valve 12 are arranged on the air inlet pipe, a sterilization air supply pipe 13 is communicated between the sterilization pipe and the vaporization cylinder, a sterilization air supply valve 14 is arranged on the sterilization air supply pipe, an exhaust pipe 15 is communicated on the sterilization pipe, an exhaust valve 16 is arranged on the exhaust pipe, a concentration detection pipe 17 is communicated between the exhaust pipe and the sterilization air supply pipe, a concentration valve 18 and a hydrogen peroxide concentration sensor 19 are arranged on the concentration detection pipe, a residue discharging pipe 20 is communicated between the air inlet pipe and the sterilization air supply pipe, and a residue discharging valve 21 is arranged on the residue discharging pipe. The air inlet valve, the residual discharge valve, the sterilization air supply valve, the exhaust valve and the concentration valve are all electromagnetic valves. A flowmeter 22 is mounted on the air inlet pipe. The infusion pump is a peristaltic pump, and a one-way valve 23 is arranged on the liquid feeding pipe. One end of the concentration detection pipe is connected to the sterilization air supply pipe between the sterilization air supply valve and the vaporization cylinder. One end of the residual discharging pipe is connected to the sterilizing air supply pipe between the sterilizing pipe and the sterilizing air supply valve, and the other end is connected to the air inlet pipe between the compressed air filter and the air inlet valve. The vaporization cylinder is internally provided with temperature sensors 24, the vaporization plate and the heating rods are arranged at the bottom of the vaporization cylinder, the heating rods are uniformly distributed and provided with a plurality of temperature sensors, and the temperature sensors are arranged at the position of the vaporization plate. A sterilization temperature sensor 25 and a hydrogen peroxide sterilization concentration sensor 26 are arranged in the sterilization tube. The two ends of the sterilizing tube are respectively connected with an inlet end cover 27 and an outlet end cover 28, and a flange 29 is arranged on the side, close to the outlet end cover, of the sterilizing tube. The induction switch 30 is arranged on the sterilizing tube near the position of the inlet end cover, and the induction head 31 is correspondingly arranged on the inlet end cover and the induction switch. The sterilizing pipe is provided with an air inlet 32 and an air outlet 33, the air inlet and the air outlet are arranged between the flange and the inlet end cover, the air inlet is connected with the sterilizing air supply pipe, and the air outlet is connected with the exhaust pipe. The compressed air filter, the vaporization cylinder, the hydrogen peroxide liquid storage bottle, the infusion pump, the flowmeter, the air inlet valve, the pressure regulating valve, the sterilization air supply valve, the exhaust valve, the concentration valve and the residual discharge valve are all arranged in the mounting box.
A sterilization method of a hydrogen peroxide transient sterilization device, comprising the steps of: a. transversely mounting a sterilizing tube on an isolator, arranging an outlet end of an outlet end cover connected with the sterilizing tube in the isolator, arranging an inlet end connected with an inlet end cover outside the isolator, fixedly connecting a flange with the isolator, opening the inlet end cover to put sterilizing substances into the sterilizing tube from the outer end of the sterilizing tube, and closing the inlet end cover; b. in the dehumidification stage, an air inlet valve, a pressure regulating valve, a sterilization air supply valve, an air outlet valve and a concentration valve are opened, a heating rod and an evaporation tray work and are preheated, and the inside of a sterilization tube is dehumidified to below 10% RH; when the temperature in the sterilizing tube reaches 37 ℃, all valves are closed, meanwhile, after the temperature on the evaporating pan reaches 140 ℃, hydrogen peroxide drops are injected through a delivery pump, an air inlet valve and a concentration valve are opened, a concentration adjusting stage is carried out, dry compressed air in the process enters a vaporizing cylinder through a compressed air filter and a pressure regulating valve, a pipeline is preheated through hot air in the vaporizing cylinder, and hydrogen peroxide gas is prevented from being contacted with a tube wall to be condensed into liquid hydrogen peroxide in the sterilizing process; c. the method comprises the steps of adjusting, setting the injection amount of hydrogen peroxide, starting a delivery pump to inject a hydrogen peroxide solution into a vaporization cylinder to vaporize the hydrogen peroxide solution to generate gaseous hydrogen peroxide, mixing the vaporized hydrogen peroxide gas with air, detecting the concentration by a hydrogen peroxide concentration sensor, discharging the mixture into an exhaust pipe, detecting the hydrogen peroxide concentration in real time by the hydrogen peroxide concentration sensor, and closing the concentration valve to enter a sterilization stage when the concentration value reaches 3000 ppm; d. in the sterilization stage, a sterilization air supply valve and an exhaust valve are opened, hydrogen peroxide gas is introduced into the sterilization tube, and the sterilization action time is 20 to 60 seconds; e. residue removal stage: closing a sterilization air supply valve, opening a residual discharge valve, an air inlet valve, a pressure regulating valve, a concentration valve and an exhaust valve, discharging residual hydrogen peroxide gas in a sterilization pipe and a vaporization cylinder by dry compressed air sent by a compressed air filter, closing all valves when the hydrogen peroxide concentration is less than 1ppm, discharging the residual, and taking out the sterilized materials from an isolator through an outlet end of the sterilization pipe. In the step a, the sterilizing pipes can be arranged obliquely or vertically, and the arrangement mode needs to install a fixing frame for fixing the sterilizing objects in the sterilizing pipes so as to prevent the sterilizing objects from sliding in the sterilizing pipes under the action of gravity.
The hydrogen peroxide transient sterilization device performs the sterilization experiments as follows:
will 10 6 Placing a Biological Indicator (BI) and a chemical indicator card into a sterilization tube;
starting a hydrogen peroxide instantaneous sterilization device to perform sterilization operation;
after the sterilization operation is finished, the BI is quickly taken out, and the un-sterilized BI and the BI after sterilization are respectively inoculated into a TSB culture medium and are cultured in an incubator at 55-60 ℃ for 7 days.
The first set of experimental parameters is as follows:
| sequence number | Humidity (RH%) | Sterilization time(s) | Sterilizing concentration (ppm) | Temperature (. Degree. C.) |
| 1 | 17.5-23 | 90 | 3323.2 | 41 |
| 2 | 17-20 | 60 | 3250 | 41.4 |
| 3 | 17-19 | 40 | 3323.4 | 41.5 |
| 4 | 18.5-20 | 20 | 2900 | 40 |
The experimental results are as follows:
the second set of experimental parameters is as follows:
| sequence number | Humidity (RH%) | Sterilization time(s) | Sterilizing concentration (ppm) | Temperature (. Degree. C.) |
| 1 | 10-21 | 60 | 1800 | 37.6 |
| 2 | 9-22 | 60 | 1856.5 | 38.6 |
| 3 | 10-21.5 | 60 | 1500 | 37.3 |
| 4 | 11-23 | 60 | 1530 | 37.9 |
| 5 | 10.5-22.5 | 60 | 1572.5 | 36.4 |
The experimental results are as follows:
as can be seen from the culture results, the Bacillus stearothermophilus 10 was obtained at a high concentration of about 3000ppm for 20 seconds 6 The sterilization effect is achieved, the sterilization temperature is about 37 ℃, and the concentration can reach 10 percent of the bacillus stearothermophilus at 1500ppm 6 And (3) a sterilization effect.
Example 2: the hydrogen peroxide instant sterilizing device (see figure 5) has a structure similar to that of the embodiment 1, and is mainly characterized in that an air inlet and an air outlet are arranged on a sterilizing pipe in the embodiment, a protective sleeve 34 is arranged at the position of the air inlet in the sterilizing pipe, a rotating shaft 35 is arranged in the protective sleeve, a plurality of driving blades 36 are uniformly arranged at one end of the rotating shaft, a rotating disc 37 is arranged at the other end of the rotating shaft, and a plurality of ventilation holes are uniformly arranged on the rotating disc; a carrying plate 38 and a slide bar 39 are arranged in the sterilizing tube, a slide block 40 is arranged on the lower surface of the slide bar, a slide rail 41 is arranged on the inner wall of the sterilizing tube, the slide block is slidably arranged on the slide rail, a plurality of pushing protrusions 42 are arranged on the upper surface of the slide bar at intervals, the pushing protrusions are of a V-shaped structure, a plurality of push rods 43 are inserted on the carrying plate at intervals, a push rod is arranged between every two adjacent pushing protrusions, the upper ends of the push rods are connected with a support bar 44, the support bar is supported on the carrying plate, and the lower ends of the push rods are attached to the upper surface of the slide bar; a connecting rod 45 is hinged between the end of the slide and the edge of the turntable. The push rod is connected with a limit rod 46 which is transversely arranged near the lower end. The push rod and the support bar are connected together to form a T-shaped structure. Other structures are the same as those of embodiment 1.
The sterilizing object is placed on the object carrying plate in the sterilizing tube, the hydrogen peroxide gas fed in from the air inlet drives the driving blade to rotate, so that the rotation of the rotating shaft is realized, the rotating disc rotates along with the rotating shaft, the rotating disc drives the sliding bar to reciprocate through the connecting rod, the pushing protrusion on the sliding bar slides over the lower end of the pushing rod, the pushing rod is lifted upwards, the sterilizing object contacted with the supporting bar is lifted upwards and even turned, and the lower surface of the sterilizing object is guaranteed to be in direct contact with the hydrogen peroxide gas, so that the sterilizing object can be rapidly sterilized. In the whole sterilization process, the sterilization objects are in a dynamic state, which is beneficial to improving the sterilization speed and the sterilization effect.
The above-described embodiments are merely preferred embodiments of the present invention, and the present invention is not limited in any way, and other variations and modifications may be made without departing from the technical aspects set forth in the claims.
Claims (8)
1. A sterilization method of a hydrogen peroxide instantaneous sterilization device is characterized in that the hydrogen peroxide instantaneous sterilization device comprises a compressed air filter, a vaporization barrel, a sterilization pipe and a hydrogen peroxide liquid storage bottle, wherein a heating rod and a vaporization disk are arranged in the vaporization barrel, a liquid conveying pipe is communicated between the hydrogen peroxide liquid storage bottle and the vaporization barrel, an infusion pump is arranged on the liquid conveying pipe, an air inlet pipe is communicated between the compressed air filter and the vaporization barrel, an air inlet valve and a pressure regulating valve are arranged on the air inlet pipe, a sterilization air conveying pipe is communicated between the sterilization pipe and the vaporization barrel, a sterilization air conveying valve is arranged on the sterilization air conveying pipe, an exhaust pipe is communicated with the sterilization air conveying pipe, an exhaust valve is arranged on the exhaust pipe, a concentration detection pipe is communicated between the exhaust pipe and the sterilization air conveying pipe, a concentration valve and a hydrogen peroxide concentration sensor are arranged on the concentration detection pipe, a residual discharging valve is arranged on the residual discharging pipe; the sterilization method comprises the following steps: a. the method comprises the steps of installing a sterilization pipe on an isolator, arranging an outlet end of the sterilization pipe in the isolator, arranging an inlet end of the sterilization pipe outside the isolator, and arranging a sterilization object into the sterilization pipe from the outer end of the sterilization pipe; b. in the dehumidification stage, an air inlet valve, a pressure regulating valve, a sterilization air supply valve, an air outlet valve and a concentration valve are opened, a heating rod and an evaporation tray work and are preheated, and the inside of a sterilization tube is dehumidified to below 10% RH; when the temperature in the sterilizing tube reaches 37 ℃, all valves are closed, meanwhile, after the temperature on the evaporating pan reaches 140 ℃, hydrogen peroxide drops are injected through a delivery pump, an air inlet valve and a concentration valve are opened, a concentration adjusting stage is carried out, dry compressed air in the process enters a vaporizing cylinder through a compressed air filter and a pressure regulating valve, a pipeline is preheated through hot air in the vaporizing cylinder, and hydrogen peroxide gas is prevented from being contacted with a tube wall to be condensed into liquid hydrogen peroxide in the sterilizing process; c. the method comprises the steps of adjusting, setting the injection amount of hydrogen peroxide, starting a delivery pump to inject a hydrogen peroxide solution into a vaporization cylinder to vaporize the hydrogen peroxide solution to generate gaseous hydrogen peroxide, mixing the vaporized hydrogen peroxide gas with air, detecting the concentration by a hydrogen peroxide concentration sensor, discharging the mixture into an exhaust pipe, detecting the hydrogen peroxide concentration in real time by the hydrogen peroxide concentration sensor, and closing the concentration valve to enter a sterilization stage when the concentration value reaches 3000 ppm; d. in the sterilization stage, a sterilization air supply valve and an exhaust valve are opened, hydrogen peroxide gas is introduced into the sterilization tube, and the sterilization action time is 20 to 60 seconds; e. residue removal stage: closing a sterilization air supply valve, opening a residual discharge valve, an air inlet valve, a pressure regulating valve, a concentration valve and an exhaust valve, discharging residual hydrogen peroxide gas in a sterilization pipe and a vaporization cylinder by dry compressed air sent by a compressed air filter, closing all valves when the hydrogen peroxide concentration is less than 1ppm, discharging the residual, and taking out the sterilized materials from an isolator through an outlet end of the sterilization pipe.
2. The sterilization method of a hydrogen peroxide transient sterilization apparatus according to claim 1, wherein the liquid feeding pipe is provided with a check valve.
3. The sterilization method of a hydrogen peroxide transient sterilization apparatus according to claim 1, wherein a temperature sensor is installed in the vaporization drum.
4. The method according to claim 1, wherein a sterilization temperature sensor and a hydrogen peroxide sterilization concentration sensor are installed in the sterilization tube.
5. The method according to claim 1, wherein the two ends of the sterilizing tube are respectively connected with an inlet end cover and an outlet end cover, and a flange is arranged on the side of the sterilizing tube close to the outlet end cover.
6. The method according to claim 5, wherein the induction switch is installed on the sterilizing tube near the inlet end cover, and the induction head is installed on the inlet end cover corresponding to the induction switch.
7. The method according to any one of claims 1 to 5, further comprising installing a cartridge, wherein the compressed air filter, the vaporization cartridge, the hydrogen peroxide liquid storage bottle, the infusion pump, the air intake valve, the pressure regulating valve, the sterilization air supply valve, the air exhaust valve, the concentration valve, and the residual discharge valve are installed in the cartridge.
8. The sterilization method of the hydrogen peroxide instantaneous sterilization device according to any one of claims 1 to 5, wherein an air inlet and an air outlet are arranged on the sterilization tube, a protective sleeve is arranged at the position of the air inlet in the sterilization tube, a rotating shaft is arranged in the protective sleeve, a plurality of driving blades are uniformly arranged at one end of the rotating shaft, and a rotating disc is arranged at the other end of the rotating shaft; a material carrying plate and a sliding bar are arranged in the sterilizing tube, a sliding block is arranged on the lower surface of the sliding bar, a sliding rail is arranged on the inner wall of the sterilizing tube, the sliding block is slidably arranged on the sliding rail, a plurality of pushing protrusions are arranged on the upper surface of the sliding bar at intervals, the pushing protrusions are of a V-shaped structure, a plurality of pushing rods are inserted on the material carrying plate at intervals, a pushing rod is arranged between every two adjacent pushing protrusions, the upper ends of the pushing rods are connected with a supporting bar, and the supporting bar is supported on the material carrying plate; and a connecting rod is hinged between the end part of the sliding strip and the edge of the turntable.
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