CN111202709A - Lactobacillus facial mask capable of inhibiting propionibacterium acnes and preparation method thereof - Google Patents
Lactobacillus facial mask capable of inhibiting propionibacterium acnes and preparation method thereof Download PDFInfo
- Publication number
- CN111202709A CN111202709A CN201911266868.5A CN201911266868A CN111202709A CN 111202709 A CN111202709 A CN 111202709A CN 201911266868 A CN201911266868 A CN 201911266868A CN 111202709 A CN111202709 A CN 111202709A
- Authority
- CN
- China
- Prior art keywords
- lactobacillus
- facial mask
- mask essence
- propionibacterium acnes
- dried powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/10—Anti-acne agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a lactobacillus facial mask essence capable of inhibiting propionibacterium acnes and a preparation method thereof; the method comprises the following steps: 5 parts of propylene glycol, 2 parts of PEG-40 hydrogenated castor oil, 1.4 parts of sodium hyaluronate, 2 parts of dimethyl glycyrrhizinate, 0.2 part of carbomer, 0.1 part of hydroxyethyl cellulose, 0.2 part of chlorphenesin, 0.05 part of geranium oil, 0.2 part of phenoxyethanol, 2.1 parts of triethanolamine, 0.5 part of inulin, 0.84 part of inactivated freeze-dried powder and the balance of water. The invention provides a lactobacillus facial mask essence for removing acnes, which is proved by tests to have an obvious inhibition effect on propionibacterium acnes, the diameter of a bacteriostatic ring can reach 17.94mM, the lactobacillus facial mask essence is insensitive to temperature and illumination, the lactobacillus facial mask essence has good stability under room temperature and natural illumination, and the diameter change of the bacteriostatic ring is less than 2mM when the lactobacillus facial mask essence is stored for two months under the room temperature and natural illumination conditions.
Description
Technical Field
The invention relates to the field of biological strains, and particularly relates to a lactobacillus mask capable of inhibiting propionibacterium acnes and a preparation method thereof.
Background
Acne is a chronic inflammatory dermatosis of hair follicle sebaceous glands, has the morbidity of 70-87 percent, is good at adolescence, and has clinical manifestations mainly including blackheads and whiteheads of face, chest and back, inflammatory papules and pustules.
The propionibacterium acnes can induce proinflammatory factors, particularly IL-1 α, to be released due to natural immunity mediated by Toll-like receptor (TLR), and acquired immunity to amplify the inflammatory process with the development of diseases, further cause inflammatory factor release and neutrophil aggregation, and can also generate polypeptide substances to directly induce or aggravate inflammation.
Some acne patients have damaged skin barrier, and long-term oral or external use of anti-acne drugs such as antibiotics can aggravate the damage of skin barrier, resulting in sensitive skin. The facial mask developed by people is added with inactivated freeze-dried powder of a lactobacillus rhamnosus LR863 fermentation product, contains antibacterial substances and active ingredients such as lactic acid, acetic acid and bacteriocin, and can play a role in repairing skin barriers while inhibiting propionibacterium acnes, so that the effect of removing acne is achieved.
Disclosure of Invention
The invention aims to provide a lactobacillus facial mask essence capable of inhibiting propionibacterium acnes and a preparation method thereof.
In a first aspect, the invention is realized by the following technical scheme:
the invention relates to a lactobacillus facial mask essence capable of inhibiting propionibacterium acnes, which comprises the following components in parts by weight:
in a second aspect, the invention also relates to a preparation method of the lactobacillus facial mask essence capable of inhibiting propionibacterium acnes, which comprises the following steps:
step one, material A, namely the components in claim 1, except carbomer, hydroxyethyl cellulose and inactivated freeze-dried powder, and uniformly mixing the rest components;
secondly, material B, the carbomer and the hydroxyethyl cellulose in each component in the claim 1 are respectively dissolved in water, stirred and heated at 80 ℃ to be fully dissolved, and then cooled to room temperature for standby;
step three, material C: the inactivated freeze-dried powder in each component in the claim 1 is fully dissolved by purified water and is uniformly stirred; and step four, uniformly mixing the material A, the material B and the material C to obtain the lactobacillus facial mask essence.
Preferably, the inactivated freeze-dried powder is inactivated freeze-dried powder of a fermentation product of lactobacillus rhamnosus LR863 (classification name: lactobacillus rhamnosus, wherein the number of a preservation registration of the strain is CGMCC NO.14410, the preservation date is 2017, 07, 12 days, and No. 3 of West Lu No.1 of the China general microbiological culture Collection Committee of China general microbiological culture Collection center, Beijing city, rising district).
Preferably, the preparation of the inactivated freeze-dried powder of the lactobacillus rhamnosus LR863 fermentation product comprises the following steps:
culturing with improved MRS culture medium, and incubating at 37 deg.C overnight;
inoculating lactobacillus rhamnosus LR863 seed liquid into 10ml of improved MRS liquid culture medium according to the inoculation amount of 3%, shaking and uniformly mixing, culturing at 37 ℃ for 24h, sequentially and continuously activating for three generations, then inoculating into 250ml of improved MRS liquid culture medium according to the inoculation amount of 3%, carrying out expanded culture, sterilizing the cultured fermentation liquid at 70 ℃ for 3h, centrifuging to collect bacterial sludge, emulsifying, and freeze-drying to obtain the LR863 fermentation product inactivated freeze-dried powder.
Preferably, the MRS medium: 20g of glucose, 10g of peptone, 6.5g of beef powder, 5g of yeast extract powder, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 2g of dipotassium hydrogen phosphate, 0.58g of MgSO4 & 7H2O 0.58, 0.25g of MnSO4 & H2O 0.25, 0.5g of cysteine hydrochloride, 801mL of Tween and 1000mL of distilled water.
The method of the invention has the following advantages:
(1) the invention provides a lactobacillus facial mask essence for removing acnes, which is proved by tests to have an obvious inhibition effect on propionibacterium acnes, the diameter of a bacteriostatic ring can reach 17.94mM, the lactobacillus facial mask essence is insensitive to temperature and illumination, the lactobacillus facial mask essence has good stability under room temperature and natural illumination, and the diameter change of the bacteriostatic ring is less than 2mM when the lactobacillus facial mask essence is stored for two months under the room temperature and natural illumination conditions.
(2) Compared with antibiotics, the lactobacillus facial mask essence added with the inactivated freeze-dried powder of the lactobacillus rhamnosus LR863 fermentation product is safer and milder, and can play a role in repairing skin barriers while inhibiting propionibacterium acnes, so that the effect of removing acne is achieved.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a bacteriostatic effect diagram of the lactic acid bacteria facial mask essence;
FIG. 2 is a graph showing the effect of the lactic acid bacteria facial mask essence on temperature stability;
FIG. 3 is a graph showing the effect of the lactic acid bacteria facial mask essence on the stability to light.
Detailed Description
The present invention will be described in detail with reference to specific examples. It should be noted that the following examples are only illustrative of the present invention, but the scope of the present invention is not limited to the following examples.
Example 1
The embodiment relates to a lactobacillus facial mask essence capable of inhibiting propionibacterium acnes, which comprises the following components in parts by weight:
in a second aspect, the invention also relates to a preparation method of the lactobacillus facial mask essence capable of inhibiting propionibacterium acnes, which comprises the following steps:
step one, material A, namely the components in claim 1, except carbomer, hydroxyethyl cellulose and inactivated freeze-dried powder, and uniformly mixing the rest components;
secondly, material B, the carbomer and the hydroxyethyl cellulose in each component in the claim 1 are respectively dissolved in water, stirred and heated at 80 ℃ to be fully dissolved, and then cooled to room temperature for standby;
step three, material C: the inactivated freeze-dried powder in each component in the claim 1 is fully dissolved by purified water and is uniformly stirred; and step four, uniformly mixing the material A, the material B and the material C to obtain the lactobacillus facial mask essence.
Test materials and methods
1 test Material
(1) The strain is as follows: lactobacillus rhamnosus LR 863; wherein the registration number of the strain is CGMCC No.14410, China general microbiological culture Collection center Beijing city, Shangyang district, Xilu No.1 Hospital No. 3. Propionibacterium acnes was purchased from North Nara, accession No. BNCC 336649.
(2) Culture medium
Improving MRS liquid culture medium: 20g of glucose, 10g of peptone, 6.5g of beef powder, 5g of yeast extract powder, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 2g of dipotassium hydrogen phosphate, 0.58g of MgSO4 & 7H2O 0.58, 0.25g of MnSO4 & H2O 0.25, 0.5g of cysteine hydrochloride, 801mL of Tween and 1000mL of distilled water.
Thioglycollate medium (containing agar): 15.0g of casein, 5.0g of yeast extract powder, 5.0g of glucose, 0.5g of sodium thioglycolate, 0.5g of L-cystine, 0.001g of resazurin, 2.5g of sodium chloride, 1000mL of distilled water and pH7.1 +/-0.2.
2 test method
2.1 Strain culture methods and sample preparation
Preparation of inactivated freeze-dried powder of lactobacillus rhamnosus LR863 fermentation product: cultured in modified MRS medium and incubated at 37 deg.C overnight. Inoculating lactobacillus rhamnosus LR863 seed solution into 10ml of improved MRS liquid culture medium according to the inoculation amount of 3%, shaking and uniformly mixing, culturing at 37 ℃ for 24h, sequentially and continuously activating for three generations, then inoculating into 250ml of improved MRS liquid culture medium according to the inoculation amount of 3%, carrying out amplification culture, sterilizing the cultured fermentation liquor at 70 ℃ for 3h, centrifuging to collect bacterial sludge, emulsifying, and freeze-drying to obtain LR863 fermentation product inactivated freeze-dried powder (hereinafter referred to as inactivated freeze-dried powder).
Culturing propionibacterium acnes: the culture was carried out anaerobically in a liquid thioglycollate medium (containing agar) at 37 ℃ in a incubator for 48 hours. The method comprises the following specific steps: inoculating the Propionibacterium acnes seed liquid to 10ml of liquid thioglycollate medium (containing agar) according to the inoculation amount of 3%, shaking and mixing uniformly, carrying out anaerobic culture at 37 ℃ for 48h, and sequentially and continuously activating for three generations for later use.
2.2 preparation of the lactobacillus facial mask:
the formula of the facial mask comprises: 5 parts of propylene glycol, 2 parts of PEG-40 hydrogenated castor oil, 1.4 parts of sodium hyaluronate, 2 parts of dimethyl glycyrrhizinate, 0.2 part of carbomer, 0.1 part of hydroxyethyl cellulose, 0.2 part of chlorphenesin, 0.05 part of geranium oil, 0.2 part of phenoxyethanol, 2.1 parts of triethanolamine, 0.5 part of inulin, 0.84 part of inactivated freeze-dried powder and the balance of water.
The preparation method comprises the following steps: the material A comprises the components except carbomer, hydroxyethyl cellulose and inactivated freeze-dried powder, which can be mixed uniformly in advance for later use. And the material B, carbomer and hydroxyethyl cellulose are respectively dissolved in water, stirred and heated to 80 ℃ for full dissolution, and then cooled to room temperature for later use. C, material C: the inactivated lyophilized powder should be dissolved in purified water and stirred well. And (3) respectively preparing the A, B and the material C, and uniformly mixing to obtain the lactobacillus mask essence.
3. Bacteriostasis test
(1) Cooling the agar culture medium containing 2.0% of the extract to about 50 ℃, pouring 10mL of the agar culture medium into a sterile plate, and airing the agar culture medium in a clean workbench;
(2) placing sterilized Oxford cup in a dish with agar, and standing for 10 min;
(3) add 1mL Propionibacterium acnes to 100mL of liquid thioglycollate medium (containing agar) cooled to 50 ℃ and mix well;
(4) pouring 10mL of the liquid thioglycollate culture medium (containing agar) on the flat plate with the Oxford cup, and clamping the Oxford cup after solidification to form an Oxford cup hole;
(5) 0.1mL of test sample is injected into each Oxford cup hole, tetracycline hydrochloride is used as a positive control, physiological saline is used as a negative control, standing is carried out for anaerobic culture for 48-72h at 37 ℃, the diameter of a bacteriostatic ring is measured by a vernier caliper, and two groups are arranged in parallel.
Preferably, the inactivated freeze-dried powder is lactobacillus rhamnosus LR863 fermentation product inactivated freeze-dried powder or propionibacterium acnes inactivated freeze-dried powder.
Preferably, the preparation of the inactivated freeze-dried powder of the lactobacillus rhamnosus LR863 fermentation product comprises the following steps:
culturing with improved MRS culture medium, and incubating at 37 deg.C overnight;
inoculating lactobacillus rhamnosus LR863 seed liquid into 10ml of improved MRS liquid culture medium according to the inoculation amount of 3%, shaking and uniformly mixing, culturing at 37 ℃ for 24h, sequentially and continuously activating for three generations, then inoculating into 250ml of improved MRS liquid culture medium according to the inoculation amount of 3%, carrying out expanded culture, sterilizing the cultured fermentation liquid at 70 ℃ for 3h, centrifuging to collect bacterial sludge, emulsifying, and freeze-drying to obtain the LR863 fermentation product inactivated freeze-dried powder.
Preferably, the MRS medium: 20g of glucose, 10g of peptone, 6.5g of beef powder, 5g of yeast extract powder, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 2g of dipotassium hydrogen phosphate, 0.58g of MgSO4 & 7H2O 0.58, 0.25g of MnSO4 & H2O 0.25, 0.5g of cysteine hydrochloride, 801mL of Tween and 1000mL of distilled water.
4. The test results of this example are:
4.1 bacteriostatic effect of the lactobacillus facial mask essence, as shown in figure 1.
The antibacterial effect of the lactobacillus facial mask essence is achieved. A is a commercially available facial mask essence, B is the lactic acid bacteria facial mask essence of the invention, C is the facial mask essence without inactivated freeze-dried powder, N is a negative control (normal saline), and P is a positive control (tetracycline hydrochloride with the concentration of 35 mu g/ml). The diameters of the inhibition zones of the various essences are shown in table 1.
TABLE 1
4.2 lactic acid bacteria facial mask essence stability to temperature, see fig. 2.
Figure 2 lactic acid bacteria facial mask essence temperature stability. A and C are respectively the bacteriostatic effect of the lactobacillus mask essence which is placed at room temperature for 0 month and 2 months, B is the mask essence without inactivated freeze-dried powder, "N" is a negative control (normal saline), and "P" is a positive control (tetracycline hydrochloride with the concentration of 35 mu g/ml). The diameters of the inhibition zones of the various essences are shown in table 2.
TABLE 2
| Diameter of bacteriostatic circle (mM) | |
| A | 20.52 |
| B | 10.86 |
| C | 19.98 |
| P | 29.64 |
| N | 0 |
4.3 lactic acid bacteria facial mask essence is stable to light, see fig. 3.
FIG. 3 shows the light stability of the lactic acid bacteria facial mask essence. A. B and C are respectively the bacteriostatic effects of the lactobacillus mask essence under natural illumination for 0 day, 1 month and 2 months, "N" is a negative control (normal saline) and "P" is a positive control (tetracycline hydrochloride with the concentration of 35 mu g/ml). The diameters of the inhibition zones of the various essences are shown in table 3.
TABLE 3
| Diameter of bacteriostatic circle (mM) | |
| A | 19.56 |
| B | 18.00 |
| C | 17.96 |
| P | 30.88 |
| N | 0 |
The method of the invention has the following advantages:
(1) the invention provides a lactobacillus facial mask essence for removing acnes, which is proved by tests to have an obvious inhibition effect on propionibacterium acnes, the diameter of a bacteriostatic ring can reach 17.94mM, the lactobacillus facial mask essence is insensitive to temperature and illumination, the lactobacillus facial mask essence has good stability under room temperature and natural illumination, and the diameter change of the bacteriostatic ring is less than 2mM when the lactobacillus facial mask essence is stored for two months under the room temperature and natural illumination conditions.
(2) Compared with antibiotics, the lactobacillus facial mask essence added with the inactivated freeze-dried powder of the lactobacillus rhamnosus LR863 fermentation product is safer and milder, and can play a role in repairing skin barriers while inhibiting propionibacterium acnes, so that the effect of removing acne is achieved.
The invention aims to provide a lactobacillus facial mask essence for removing acnes, which is proved by tests to have an obvious inhibition effect on propionibacterium acnes, the diameter of a bacteriostatic circle can reach 17.94mM, the lactobacillus facial mask essence is insensitive to temperature and illumination, the lactobacillus facial mask essence has good stability under room temperature and natural illumination, and the diameter change of the bacteriostatic circle is less than 2mM when the lactobacillus facial mask essence is stored for two months under the room temperature and natural illumination conditions. Compared with antibiotics, the lactobacillus facial mask essence added with the inactivated freeze-dried powder of the lactobacillus rhamnosus LR863 fermentation product is safer and milder, and can play a role in repairing skin barriers while inhibiting propionibacterium acnes, so that the effect of removing acne is achieved.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention.
Claims (6)
1. The lactobacillus facial mask essence capable of inhibiting propionibacterium acnes is characterized by comprising the following components in parts by weight:
2. the method for preparing the lactobacillus facial mask essence capable of inhibiting propionibacterium acnes according to claim 1, wherein the method comprises the following steps:
step one, material A, namely the components in claim 1, except carbomer, hydroxyethyl cellulose and inactivated freeze-dried powder, and uniformly mixing the rest components;
secondly, material B, the carbomer and the hydroxyethyl cellulose in each component in the claim 1 are respectively dissolved in water, stirred and heated at 80 ℃ to be fully dissolved, and then cooled to room temperature for standby;
step three, material C: the inactivated freeze-dried powder in each component in the claim 1 is fully dissolved by purified water and is uniformly stirred;
and step four, uniformly mixing the material A, the material B and the material C to obtain the lactobacillus facial mask essence.
3. The lactic acid bacteria facial mask essence capable of inhibiting propionibacterium acnes of claim 1, wherein the inactivated freeze-dried powder is lactobacillus rhamnosus LR863 fermentation product inactivated freeze-dried powder.
4. The lactic acid bacteria facial mask essence capable of inhibiting propionibacterium acnes according to claim 3, wherein the lactobacillus rhamnosus LR863 fermentation product inactivated freeze-dried powder is prepared by the following steps:
culturing with improved MRS culture medium, and incubating at 37 deg.C overnight;
inoculating lactobacillus rhamnosus LR863 seed liquid into 10ml of improved MRS liquid culture medium according to the inoculation amount of 3%, shaking and uniformly mixing, culturing at 37 ℃ for 24h, sequentially and continuously activating for three generations, then inoculating into 250ml of improved MRS liquid culture medium according to the inoculation amount of 3%, carrying out expanded culture, sterilizing the cultured fermentation liquid at 70 ℃ for 3h, centrifuging to collect bacterial sludge, emulsifying, and freeze-drying to obtain the LR863 fermentation product inactivated freeze-dried powder.
5. The lactic acid bacteria mask essence capable of inhibiting propionibacterium acnes according to claim 4, wherein the MRS medium: 20g of glucose, 10g of peptone, 6.5g of beef powder, 5g of yeast extract powder, 5g of sodium acetate, 2g of diammonium hydrogen citrate, 2g of dipotassium hydrogen phosphate and MgSO4·7H2O 0.58g，MnSO4·H20.25g of O, 0.5g of cysteine hydrochloride, 801mL of Tween and 1000mL of distilled water.
6. The lactic acid bacteria mask essence capable of inhibiting propionibacterium acnes according to claim 4, wherein the thioglycollate medium: 15.0g of casein, 5.0g of yeast extract powder, 5.0g of glucose, 0.5g of sodium thioglycolate, 0.5g of L-cystine, 0.001g of resazurin, 2.5g of sodium chloride, 1000mL of distilled water and pH7.1 +/-0.2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911266868.5A CN111202709A (en) | 2019-12-11 | 2019-12-11 | Lactobacillus facial mask capable of inhibiting propionibacterium acnes and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911266868.5A CN111202709A (en) | 2019-12-11 | 2019-12-11 | Lactobacillus facial mask capable of inhibiting propionibacterium acnes and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111202709A true CN111202709A (en) | 2020-05-29 |
Family
ID=70782634
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911266868.5A Pending CN111202709A (en) | 2019-12-11 | 2019-12-11 | Lactobacillus facial mask capable of inhibiting propionibacterium acnes and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111202709A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010013179A1 (en) * | 2008-07-29 | 2010-02-04 | L'oreal | Cosmetic use of microorganisms for the treatment of oily skin |
| CN106011024A (en) * | 2016-07-11 | 2016-10-12 | 广州丹奇日用化工厂有限公司 | Micrococcus microcapsule and application of micrococcus microcapsule to acne removing cosmetics |
| KR20160118486A (en) * | 2015-04-02 | 2016-10-12 | 주식회사 씨앤비코스메틱 | Cosmetic composition for anti-acne and atophy remedy containing mixed fermented grain seed by using lactic acid bacteria |
| CN108048347A (en) * | 2017-12-06 | 2018-05-18 | 河北然生物科技有限公司 | Lactobacillus rhamnosus, lactobacillus rhamnosus preparation and application thereof |
| CN110507598A (en) * | 2019-09-10 | 2019-11-29 | 河北一然生物科技有限公司 | Lactic acid bacteria is inhibiting the application in propionibacterium acnes and skin care item made of it |
-
2019
- 2019-12-11 CN CN201911266868.5A patent/CN111202709A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010013179A1 (en) * | 2008-07-29 | 2010-02-04 | L'oreal | Cosmetic use of microorganisms for the treatment of oily skin |
| KR20160118486A (en) * | 2015-04-02 | 2016-10-12 | 주식회사 씨앤비코스메틱 | Cosmetic composition for anti-acne and atophy remedy containing mixed fermented grain seed by using lactic acid bacteria |
| CN106011024A (en) * | 2016-07-11 | 2016-10-12 | 广州丹奇日用化工厂有限公司 | Micrococcus microcapsule and application of micrococcus microcapsule to acne removing cosmetics |
| CN108048347A (en) * | 2017-12-06 | 2018-05-18 | 河北然生物科技有限公司 | Lactobacillus rhamnosus, lactobacillus rhamnosus preparation and application thereof |
| CN110507598A (en) * | 2019-09-10 | 2019-11-29 | 河北一然生物科技有限公司 | Lactic acid bacteria is inhibiting the application in propionibacterium acnes and skin care item made of it |
Non-Patent Citations (4)
| Title |
|---|
| 王培义等: "《化妆品--原理配方生产工艺》", 28 February 2014, 化学工业出版社 * |
| 王昌涛等: "《化妆品植物添加剂的开发与应用》", 30 April 2013, 中国轻工业出版社 * |
| 马振友等: "《皮肤美容化妆品制剂手册》", 31 January 2015, 中医古籍出版社 * |
| 魏成广等: "《中国钾盐工业概览》", 31 July 2009, 上海交通大学出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2022236935A1 (en) | Probiotic having effect of inhibiting growth of proteus mirabilis, and fermentation broth and use thereof | |
| CN112940968A (en) | Lactobacillus fermentum, lactobacillus fermentum culture and preparation method thereof | |
| CN113773984B (en) | Lactobacillus rhamnosus for improving rhizoma Polygonati polysaccharide yield and regulating skin barrier and immunity | |
| CN109497558A (en) | Kuh-seng dregs of a decoction polysaccharide synbiotic and the preparation method and application thereof | |
| WO2021000247A1 (en) | Strain for producing nattokinase and production method therefor | |
| CN112159778B (en) | Bifidobacterium animalis capable of relieving psoriasis and application thereof | |
| CN116656542A (en) | Lactobacillus mucilaginosus with function of relieving alcoholic liver injury and application thereof | |
| CN119120263A (en) | A strain of Lactobacillus pentosus JNL0030, its application and a microbial preparation | |
| CN102021130A (en) | Clostridium bifermentans for generating equol by degrading daidzein and bacteria agent and application thereof | |
| CN117535208B (en) | Lactobacillus crispatus and application thereof in female genital tract health | |
| CN111202709A (en) | Lactobacillus facial mask capable of inhibiting propionibacterium acnes and preparation method thereof | |
| CN116059267B (en) | Preparation method and application of bacillus subtilis IOB430 fermented metapowder | |
| CN117860833A (en) | Composite probiotic metafermentation product for relieving damage of micro-plastics to intestinal tracts of organisms and application thereof | |
| CN111567807A (en) | Rhamnose-containing lactobacillus composition for inhibiting inflammatory reaction and resisting vaginitis and application thereof | |
| CN107267411A (en) | A kind of non-glutamic acid dependent form producing strains and its fermentation process that γ polyglutamic acids are produced for hot fermentation | |
| CN116948901A (en) | Application of Weissella sinustrophus D-2 exopolysaccharide in inhibiting colon cancer cells | |
| CN115386513A (en) | Probiotics capable of producing hyaluronic acid, helping skin cells to produce hyaluronic acid and preventing photoaging and application of probiotics | |
| CN116875491A (en) | Bifidobacterium animalis JWA63 with weight-losing, lipid-lowering and growth-promoting functions and application thereof | |
| CN116531413A (en) | Application of lactic acid bacteria postbiotics in the preparation of drugs for preventing and/or treating alcoholic liver disease | |
| CN115553380A (en) | A kind of fermented feed and its fermentation process | |
| CN115317432A (en) | Facial mask containing bifidobacterium lactis fermentation product | |
| CN114231576A (en) | Application of lactobacillus reuteri in preparation of plant polysaccharide | |
| CN109874329A (en) | A kind of production butyric acid is dwelt bacillus faecalis and its cultural method and application | |
| CN117535209B (en) | Lactobacillus brevis and application thereof in female genital tract health | |
| CN117070425B (en) | Technological method for improving metabolic stability of probiotics in organism and probiotics freeze-dried powder |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 050000 No.16, East bangxiu Road, North Zone, Zhengding high tech Industrial Development Zone, Zhengding District, China (Hebei) pilot Free Trade Zone, Shijiazhuang City, Hebei Province Applicant after: Hebei Yiran Biotechnology Co.,Ltd. Address before: 050000 No.16, bangxiu East Road, North District, high tech Industrial Development Zone, Zhengding County, Shijiazhuang City, Hebei Province Applicant before: HEBEI INATURAL BIOTECHNOLOGY Co.,Ltd. |
|
| CB02 | Change of applicant information | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200529 |
|
| RJ01 | Rejection of invention patent application after publication |