CN111139190A - Monascus strain, fermented soybean meal thereof and functional biological feed for aquatic products - Google Patents
Monascus strain, fermented soybean meal thereof and functional biological feed for aquatic products Download PDFInfo
- Publication number
- CN111139190A CN111139190A CN202010101130.XA CN202010101130A CN111139190A CN 111139190 A CN111139190 A CN 111139190A CN 202010101130 A CN202010101130 A CN 202010101130A CN 111139190 A CN111139190 A CN 111139190A
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- Prior art keywords
- monascus
- soybean meal
- aquatic
- feed
- biological feed
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- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
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- A—HUMAN NECESSITIES
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention belongs to the field of biological feeds, and relates to monascus, a biological feed prepared by fermenting the monascus, and application of the monascus biological feed in aquaculture. The monascus in the invention can produce various functional metabolites such as monascus pigment, protease, amylase, lipase, ergosterol, statins, gamma-aminobutyric acid and the like, and the fermentation product contains small peptides. The biological feed is applied to aquaculture, and can significantly improve the survival rate and immune function of aquatic animals and improve the growth performance.
Description
Technical Field
The invention belongs to the field of biological feeds, and relates to monascus, a biological feed prepared by fermenting the monascus, and application of the monascus biological feed in aquaculture.
Background
Monascus (Monascus), Ascomycetes (Ascomycetes), Euascomycetes (Euascomycetes), and Monascus (Monascus). The monascus is saprophytic fungi, has the optimal growth pH of 3.5-5, can resist pH 3.5, and is particularly acidophilic to lactic acid. The growth temperature is 26-42 ℃, the optimal temperature is 32-35 ℃, and the growth temperature can tolerate 10% ethanol. Monascus is of interest because it produces large amounts of natural haematochrome.
The monascus has a long history of production and use in China, has high medicinal value, and can be used for brewing wine and making vinegar. Monascus produces many enzymes, mainly including amylase, protease, esterases, and cellulase. At present, monacolin is widely used in China, and is mainly used as a component of a health-care product, a natural pigment and the like. Meanwhile, the monascus fermentation product can be used as a green feed additive for livestock and poultry, and has the advantages of no toxicity, no harm, no residue, no resistance, no environmental pollution, low cost and the like. The application of the monascus fermentation product in livestock and poultry is mainly used for improving the quality of poultry eggs, increasing the color of egg yolks, improving the color of muscles, reducing cholesterol and the like, and no relevant report is found in the field of aquatic products.
Disclosure of Invention
The invention aims to provide application of an monascus strain, an monascus fermented product and an aquatic functional biological feed in improving the immunity of aquatic animals, improving the survival rate of the aquatic animals and improving the production performance of the aquatic animals.
Firstly, the invention provides an Monascus sp which can be used for fermenting soybean meal and has the following preservation number: CGMCC No. 17075.
The invention also provides fermented soybean meal prepared by fermenting the monascus.
The invention also provides a preparation method of the fermented soybean meal, which comprises the following steps: inoculating the monascus seed solution on sterilized soybean meal with the water content of 35-40% according to the inoculation amount of 10-30% by mass, uniformly mixing, and culturing for 5-10 days at 28-32 ℃, wherein the humidity is controlled to be 30-50%.
The invention also provides an aquatic functional biological feed containing the monascus.
The invention also provides an aquatic functional biological feed containing the fermented soybean meal.
The invention also provides a preparation method of the aquatic functional biological feed, which is characterized in that the fermented soybean meal is mixed with the raw materials of the aquatic feed according to the mass ratio of 0.5-5.0%, and the aquatic functional biological feed is prepared according to the processing technology of the aquatic feed.
The invention also provides application of the monascus in improving the immunity of aquatic animals, improving the survival rate of the aquatic animals and improving the production performance of the aquatic animals.
Wherein the aquatic animals include, but are not limited to, shrimp, crab, sea fish, and freshwater fish.
The monascus in the invention can produce a plurality of functional metabolites such as monascus pigment, protease, statin substances, amylase, lipase, ergosterol, gamma-aminobutyric acid and the like, and the fermentation product contains small peptides, and the color yield is obviously higher than that of other strains screened and cultured under the same conditions, and the monascus is identified as the monascus.
The protein proportion of the soybean meal fermented by monascus is obviously increased, the content of acid soluble protein is obviously superior to that of the soybean meal raw material, the proportion of anti-nutritional factors is obviously reduced, and the color value can be maintained at a higher level. The monascus fermented soybean meal fed to the penaeus vannamei boone has better growth index and immunity index results, which shows that the growth of the penaeus vannamei boone can be promoted and the immunity of the penaeus vannamei boone can be enhanced by increasing the adding amount of the monascus fermented soybean meal in the feed, and the growth of the grouper can also be promoted and the immunity can be enhanced by properly adding the soybean meal monascus fermented matter fed to the grouper. The clown fish is fed by the bean pulp monascus fermentation product, so that the clown fish is brighter in body color and has higher ornamental value.
Drawings
FIG. 1 shows the developmental clade of strain M-21.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1 selection of Monascus
The strain source is as follows: is taken from Fujian Gutian area and is a leaven (five types) for making red vinasse acid by residents.
And (3) separation of monascus:
pulverizing two different red yeast rice grains into powder, spreading on PDA culture medium with tripod, culturing at 30 deg.C for 3-4 days, selecting grown red yeast rice, shaking in sterile water test tube, and further purifying by dilution plate method. Adding the purified monascus spore liquid into a centrifugal tube containing sterilized glycerol, and storing at the low temperature of-80 ℃.
Liquid fermentation monascus:
adding 10% monascus spore liquid into 100mL of liquid culture medium, culturing for 10d at 30 ℃ and 180r/min, wherein the formula of the liquid culture medium is as follows: 10% of glucose, 1% of peptone, 0.2% of sodium nitrate, 0.2% of potassium dihydrogen phosphate, 0.05% of magnesium sulfate heptahydrate and 0.01% of calcium chloride.
The method for detecting the metabolic product indexes of 5 monascus strains separated from red yeast rice produced by the Gutian manufacturers comprises the following steps:
the protease detection method comprises the following steps: reference is made to the protease activity assay in SB/T10317-1999.
The lipase detection method comprises the following steps: refer to the determination of lipase activity in GB/T23535-2009.
An amylase detection method refers to the determination of α -amylase activity in GB/T5521-2008.
Ergosterol detection method: the ergosterol content in red rice is determined by high performance liquid chromatography.
The statin detection method comprises the following steps: the high performance liquid chromatography is adopted to determine the lovastatin content in the monascus fermented product.
The detection method of gamma-aminobutyric acid comprises the following steps: and (3) determining the content of gamma-aminobutyric acid in the monascus fermentation product by adopting a high performance liquid chromatography.
And (3) measuring the color value of the fermentation liquor:
and leaching the fermentation liquor for 3 hours by using a 75% ethanol solution, centrifuging to obtain a supernatant, using the 75% ethanol solution as a blank control, and measuring the absorbance of the diluted filtrate at the wavelength of 510 nm.
Color value of fermentation broth OD510nmX dilution factor (U/mL)
The index measurement results are as follows:
TABLE 1 results of the determination of the isolated Monascus metabolites
As shown in Table 1, the activities of protease, amylase and lipase of the monascus M-21 are higher than those of other strains, the contents of ergosterol, lovastatin and gamma-aminobutyric acid are slightly higher than those of other strains, and the color yield is obviously higher than that of other strains, so the monascus M-21 is selected for ITS identification and subsequent research.
The sequence of the strain is compared with other Monascus in GenBank and subjected to homology analysis, a developmental evolutionary tree of the strain is drawn (figure 1), and finally the strain M-21 is identified as the Monascus sp (the ITS sequence of the strain M-21 is shown in SEQ ID No. 1).
The strain is preserved in the China general microbiological culture Collection center in 2019, 2 months and 25 days, and the address is as follows: the microbial research institute of the national academy of sciences No. 3, Xilu No.1, Beijing, Chaoyang, and the preservation numbers are as follows: CGMCCNo.17075.
EXAMPLE 2 Monascus seed liquid culture
Optimizing the culture temperature of the seed liquid:
inoculating the slant of the stored monascus test tube into 5 liquid seeds containing 100mLPlacing the medium in a 500mL triangular flask respectively in shaking tables with the culture temperature of 26 ℃, 28 ℃, 30 ℃, 32 ℃ and 34 ℃ and the rotation speed of 250r/min for culturing for 3 days, and determining the influence of different culture temperatures on the yield of the monascus pigment, wherein the formula of the liquid seed culture medium is as follows: corn starch 3.0%, cottonseed protein 1.0%, K2HPO4·3H2O 1.0％，ZnSO4·7H2O 1.0％，MgSO4·7H2O 0.5％，pH 7.0。
The method for measuring the color value of the seed liquid comprises the following steps:
absorbing 1mL of seed liquid, adding the seed liquid into a test tube filled with 9mL of 75% ethanol solution (pH is 6.0-7.0), shaking uniformly, centrifuging after 3 hours, taking supernate, taking 75% ethanol solution as blank control, measuring light absorption (A) values at the wavelengths of 510nm and 410nm, and multiplying the light absorption value by the dilution multiple of the seed liquid to obtain the color value (U/mL) of the red pigment and the yellow pigment of the seed liquid, wherein the total color value is the sum of the two.
A (color value of seed liquid) is A510nm+A410nm
As shown in Table 2, it was found that the productivity of the pigment was the highest when the culture temperature of Monascus purpureus CGMCC No.17075 was 30 ℃.
TABLE 2 Effect of different culture temperatures on color number
| Culture temperature (. degree.C.) | 26 | 28 | 30 | 32 | 34 |
| Color value (U/mL) | 562.3 | 884.4 | 1457.5 | 1021.6 | 832.7 |
Preparing a seed solution:
inoculating the stored monascus test tube slant into a 500mL triangular flask containing 100mL liquid seed culture medium, and culturing in a shaking table at 30 ℃ and 250r/min for 3 days to obtain the seed liquid.
Example 3 cultivation of Monascus fermentate of soybean meal
The monascus seed solution prepared in example 1 was inoculated on a sterilized soybean meal medium with a water content of 35% to 40% in an inoculum size of 20%, evenly mixed, spread out in a shallow tray, cultured in a thermostatic chamber at 30 ℃ for 7 days with a humidity controlled at 40%, and dried by drying on a filter bed after the solid state fermentation. And crushing the fermented material to 60 meshes by using a crusher. Carrying out conventional detection on the fermented soybean meal finished product, wherein the detection method and indexes are as follows:
the crude protein detection method comprises the following steps: refer to a method for detecting crude protein in GB/T6432-2018 feed.
The crude fiber detection method comprises the following steps: refer to a method for detecting crude fibers in GB/T6434-2006 fodder.
The water content detection method comprises the following steps: refer to the measurement of the water content in GB/T6435-.
The ash content detection method comprises the following steps: refer to a method for detecting crude ash in GB/T6438-.
The acid soluble protein detection method comprises the following steps: refer to a detection method of acid soluble protein in GB/T22492-.
A method for detecting glycinin and β -conglycinin comprises adopting rapid detection kit for soybean antinutritional factor in ark of Beijing Longke family, and the operation method refers to the instruction.
The detection method of urease activity comprises the following steps: refer to the measurement of urease activity in the soybean product for feed of GB/T8622-2006.
Color value: accurately weighing 0.2g of the crushed fermentation material, dissolving the crushed fermentation material by using 70% ethanol solution, transferring the solution into a 100mL volumetric flask to fix the volume to a scale, soaking the volumetric flask in water bath at 60 ℃ for 1h, taking out the solution to cool to room temperature, filtering the solution by using filter paper, accurately sucking 2.0 mL-5.0 mL of filtrate into a 50mL volumetric flask (enabling the absorbance of the final diluent to fall within the range of 0.3-0.6), diluting the solution by using 70% ethanol solution to fix the volume to 50mL, using 70% ethanol solution as reference, and measuring the absorbance A of the sample soaking diluent under the wavelength of 505 nm.
The color number X (U/g) is calculated according to the following formula:
X＝A×100/m×50/V
in the formula:
a-absorbance of the soaking dilution;
100-conversion factor;
m-weighing the mass of the sample, wherein the unit is gram (g);
50-a conversion factor;
v-volume of ethanol soak solution in milliliters (mL)
The results are shown in table 3, the acid soluble protein content of the monascus CGMCC No.17075 after the bean pulp solid fermentation is carried out is obviously higher than that of the bean pulp raw material, the anti-nutritional factor (glycinin, β -conglycinin and urease activity) proportion of the fermented bean pulp is obviously reduced, the protein proportion of the fermented bean pulp is obviously increased after the fermentation, and the color value of the obtained fermented material can reach 1493.1U/g.
TABLE 3 results of solid fermentation
Example 4 application of fermented product of soybean meal monascus in culture of penaeus vannamei
The penaeus vannamei boone feed for the test is purchased from a Zhaan Meiling breeding base at a test site, individuals with normal ingestion, consistent specification, strong physique, active ingestion and no damage and disease are selected as test materials, the initial average weight is about 1.40g, the formula of the penaeus vannamei boone feed for the test is 30% of soybean meal, 30% of fish meal, α% of starch 5%, 5% of squid cream, 4% of gluten, 1% of soybean oil, 1% of fish oil, 2% of lecithin, 0.10% of vitamin C phosphate, 0.50% of choline chloride, 1.00% of sodium alginate, 0.40% of multivitamin, 0.40% of multiore, 1% of monocalcium phosphate and 18.60% of flour, the fermented soybean meal monascus is mixed with the raw materials of the base feed according to the addition amount of 0.50%, the penaeus vannamei boone feed is processed into the penaeus vannamei boone feed through the processes of crushing, modulation, granulation, cooling, powder removal and the like, the test is divided into two groups, the test groups are respectively fed, the test is carried out.
The test indexes are as follows:
weight gain WGR (%) (final average weight-initial average weight) × 100/initial average weight)
Survival rate SR (%). Final shrimp mantissa × 100/initial shrimp mantissa
The feed coefficient FCR ═ feed feeding amount/(final shrimp weight-initial shrimp weight)
Superoxide dismutase (SOD), Catalase (CAT) in liver and pancreas, and Lysozyme (LZM) in blood serum.
The results are shown in Table 4: compared with a control group, the penaeus vannamei added with the bean pulp monascus fermentation product can obviously improve the growth speed and the conversion rate of the penaeus vannamei, and is also superior to the control group in terms of the enzyme activities of liver superoxide dismutase (SOD), Catalase (CAT) and Lysozyme (LZM) in serum. The results show that the bean pulp monascus fermentation product can promote the growth of the penaeus vannamei boone and improve the enzyme activity of liver superoxide dismutase (SOD), Catalase (CAT) and Lysozyme (LZM) in serum.
TABLE 4 influence of Monascus fermentation product of soybean meal on growth and immunity index of Penaeus vannamei Boone
Example 5 application of Monascus fermentation product of soybean meal in Penaeus vannamei Boone culture
Example 5 is different from example 4 in that the addition amount of the fermented soybean meal monascus was 2.00%.
The results are shown in Table 5: compared with a control group, the feed added with 2.00 percent of the bean pulp monascus fermentation material for feeding the penaeus vannamei boone can obviously improve the growth speed, the conversion rate and the survival rate of the penaeus vannamei boone, and can also improve the enzyme activity of liver superoxide dismutase (SOD), Catalase (CAT) and Lysozyme (LZM) in serum, and is also superior to the test result of the feed added with 0.50 percent of the monascus fermentation bean pulp for feeding the penaeus vannamei boone in the example 3, which shows that the increase of the addition amount of the bean pulp monascus fermentation material in the feed can promote the growth of the penaeus vannamei boone and enhance the immunity of the penaeus vannamei boone.
TABLE 5 influence of Monascus fermentation product of soybean meal on growth and immunity index of Penaeus vannamei Boone
Example 6 application of Monascus fermentation product of soybean meal in Penaeus vannamei Boone culture
Example 6 differs from examples 4 and 5 in that the amount of the soybean meal monascus fermentate added was 5.00%.
The results are shown in Table 6: compared with a control group, the feed added with 5.00 percent of the bean pulp monascus leavening can obviously improve the growth speed, the conversion rate and the survival rate of the penaeus vannamei, the enzyme activities of superoxide dismutase (SOD), Catalase (CAT) and Lysozyme (LZM) in serum in liver are also greatly improved, compared with the test results of feeding the penaeus vannamei by adding 0.50 percent and 2.00 percent of the bean pulp monascus leavening in the feed in the examples 3 and 4, the growth index and the immunity index result are better, but the growth amplitude is obviously reduced, which shows that the increase of the addition amount of the bean pulp monascus leavening in the feed can promote the growth of the penaeus vannamei and enhance the immunity of the penaeus vannamei. Therefore, the effect of promoting the growth of the penaeus vannamei boone and enhancing the immunity of the penaeus vannamei boone is best and obvious when the addition amount of the soybean meal monascus fermented product is 5.00 percent.
TABLE 6 influence of Monascus fermentation product of soybean meal on growth and immunity index of Penaeus vannamei Boone
Example 7 application of Monascus fermentation product of soybean meal in grouper cultivation
The test site is at Zhaan Meiling cultivation base. The test grouper is purchased from a base, and individuals with normal food intake, no injury and no disease are selected as test materials, the initial average weight is about 22.61g, and the average body length is 10.62 cm. The basic feed formula of the grouper comprises the following components: 450.00 per mill of fish meal, 285.00 per mill of soybean meal, 120.00 per mill of flour, 30.00 per mill of chicken powder, 20.00 per mill of porcine hemoglobin powder, 20.00 per mill of fish oil, 15.00 per mill of soybean phospholipid oil, 20.00 per mill of soybean oil, 15.00 per mill of monocalcium phosphate, 2.00 per mill of choline chloride, 1.5 per mill of multi-vitamin of marine fish, 1.5.00 per mill of multi-mineral of marine fish and 20.00 per mill of bentonite. Mixing the bean pulp monascus fermentation product with the basic feed raw material according to the addition amount of 3.00% by mass ratio, and processing the mixture into the grouper feed through the processes of crushing, modulating, granulating, cooling, removing powder and the like. The test is divided into two groups, each group is provided with four repetitions, each repetition is used for culturing 20 groupers in experimental jars with the same specification, two groups of groupers are fed with two test feeds respectively, the test group is fed with monascus fermentate, and the control group is fed with basic feed for 10 weeks. And after the experiment is finished, measuring and calculating related indexes, and observing and recording the death condition in the whole process.
The test indexes are as follows:
weight gain WGR (%) (final average weight-initial average weight) × 100/initial average weight)
Survival rate SR (%). Final fish tail number × 100/initial fish tail number
The feed coefficient FCR is the feed feeding amount/(final fish weight-initial fish weight)
Superoxide dismutase (SOD), Catalase (CAT) in the liver and pancreas,
Enzymatic activity of Lysozyme (LZM) in serum.
The results are shown in Table 7: the growth speed and the conversion rate of the grouper fed by adding the soybean meal monascus fermentate into the feed are obviously improved compared with those of a control group fed by basic feed, and the grouper is also superior to the control group in terms of the enzyme activities of liver superoxide dismutase (SOD), Catalase (CAT) and Lysozyme (LZM) in serum. The results show that the bean pulp monascus fermentation product can promote the growth of the groupers and improve the immunity of the groupers.
TABLE 7 influence of Monascus fermentation of soybean meal on growth and immunity index of grouper
Example 8 application of Monascus fermentation product of soybean meal in clown fish culture
Individuals with normal ingestion, no injury and no disease are selected as test materials, the initial weight is about 0.25g, and the average body length is 22.6 mm. The basic feed formula of the clown fish is as follows: 46.00 percent of fish meal, 20.00 percent of soybean meal, 24.60 percent of flour, 3.00 percent of wheat gluten, 2.00 percent of fish oil, 2.00 percent of soybean lecithin, 1.00 percent of calcium dihydrogen phosphate and 0.50 percent of choline. The bean pulp monascus fermentation product is mixed with the basic feed raw material according to the addition of 3.00%, and the clown fish feed is processed by the processes of crushing, modulating, granulating, cooling, removing powder and the like. The test is divided into three groups, four repeats are set in each group, 15 clown fishes are placed in test jars with the same specification in each repeat, three groups of clown fishes are fed with three test feeds respectively, the test groups are fed with bean pulp monascus leavening feed, the negative control groups are fed with basic feed, and the positive control groups are fed with high-capacity company MARINES feed which is purchased in the market and used for color aiding of the clown fishes and are bred for 4 weeks. After the experiment was completed, the body color was measured with a spectrophotometer. The L (brightness), a (redness) and b (yellowness) values of the chest yellow band and the abdomen yellow band of each fish are recorded respectively, and each part is measured three times to reduce the measurement error.
The results are shown in Table 8: compared with a control group, the clown fish added with the bean pulp monascus fermentate can obviously reduce the brightness values of the chest and the abdomen and improve the redness value and the yellowness value of the chest and the abdomen. The influence of the bean pulp monascus fermentation product on the clown fish yellow belt is obvious, the yellow belt color is obviously darker and purer, and the bean pulp monascus fermentation product has the effects of enabling the clown fish body color to be more bright and having higher ornamental value.
TABLE 8 measurement results of body color of clown fish
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Sequence listing
<110> Fujian Dabei agricultural aquatic products science Co Ltd, Beijing Dabei agricultural technology group Co Ltd
<120> Monascus fermentation product and aquatic functional biological feed thereof
<160>1
<170>SIPOSequenceListing 1.0
<210>1
<211>503
<212>DNA
<213> Monascus (Monascus sp.)
<400>1
gggaaaccta cctgatccga ggtcacctaa ggaaaaaaag gttggagagg gcaaaggccc 60
cggcccgacc tactgagcgg gtgacaaagc cccatacgct cgaggaccgg acgcggcgcc 120
gccactgcct ttcgggcccg tccccgttgc ccggaggcgc aggggacggc ggcccaacac 180
acaagccgcg cttgaggggc agtaatgacg ctcggacagg catgcccccc ggaataccag 240
ggggcgcaat gtgcgttcaa agattcgatg attcactgaa ttctgcaatt cacattactt 300
atcgcatttc gctgcgttct tcatcgatgc cggaaccaag agatccgttg ttgaaagttt 360
taaccgattt ggtatgttta ctcagacagc aatccttttc aaagacagcg ttcgagaaga 420
tgtctccggc gggccccagg gggccgcgcc gaagcaacag gaggtacaat aatcacgggt 480
gggaggttgg tcccacgaag gga 503
Claims (8)
1. Monascus (Monascus sp.), with the preservation number as follows: CGMCC No. 17075.
2. A fermented soybean meal produced by fermentation of the Monascus purpureus went according to claim 1.
3. The method for producing fermented soybean meal according to claim 2, characterized by comprising the steps of: inoculating the monascus seed solution of claim 1 on sterilized soybean meal with 35% -40% water content according to the inoculation amount of 10-30% by mass, uniformly mixing, and culturing at 28-32 ℃ for 5-10 days with the humidity controlled at 30-50%.
4. An aquatic functional biological feed comprising the monascus purpureus of claim 1.
5. An aquatic functional biological feed comprising the fermented soybean meal of claim 2.
6. The method for preparing an aquatic functional biological feed according to claim 5, wherein the fermented soybean meal according to claim 5 is mixed with aquatic feed raw materials in a mass ratio of 0.5% to 5.0%, and the mixture is processed into the aquatic functional biological feed according to an aquatic feed processing technology.
7. The use of the Monascus purpureus went of claim 1 for enhancing the immunity, survival rate, productivity and body surface and meat quality of aquatic animals.
8. The use of claim 7, wherein the aquatic animal includes, but is not limited to, shrimp, crab, marine fish, freshwater fish.
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Address after: 363502 Jindu Industrial Concentration Zone, Zhaoan County, Zhangzhou City, Fujian Province Patentee after: Fujian Dabei nonghuayou Aquatic Technology Group Co.,Ltd. Patentee after: BEIJING DABEINONG TECHNOLOGY GROUP Co.,Ltd. Address before: 363502 Jindu Industrial Concentration Zone, Zhaoan County, Zhangzhou City, Fujian Province Patentee before: FUJIAN DABEINONG FISHERIES SCIENCE & TECHNOLOGY Co.,Ltd. Patentee before: BEIJING DABEINONG TECHNOLOGY GROUP Co.,Ltd. |