CN111109433A - Compound biological preparation for regulating intestinal health of sows and preparation method and application thereof - Google Patents
Compound biological preparation for regulating intestinal health of sows and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a compound biological preparation for regulating the intestinal health of sows and a preparation method and application thereof, wherein the compound biological preparation takes bacillus licheniformis, enterococcus faecium, lactobacillus plantarum and mannanase as main components, and the viable count of the bacillus licheniformis is 1.0 multiplied by 108‑1.0×1010CFU/g, viable count of enterococcus faecium 1.0 × 108‑1.0×1010CFU/g, viable count of Lactobacillus plantarum 1.0 × 108‑1.0×1010CFU/g, the enzyme activity of the mannanase is 1000-10000U/g. The invention scientifically combines and synergistically applies the probiotics and the enzyme preparation, can fully play the regulating effect of the probiotics on the intestinal tracts of animals, and is used for feedingThe field of the material additive has higher application value.
Description
Technical Field
The invention relates to the technical field of feeds, in particular to a compound biological preparation for regulating the intestinal health of sows and a preparation method and an application method thereof.
Background
The intestinal tract is an important digestive and immune organ of animals and has the functions of digesting food, absorbing required nutrient components, protecting immunity and the like. The aim of animal husbandry is to pursue high productivity of animals, the health condition and the productivity of sows are main influence factors of economic benefits of pig farms, and the basis for obtaining high productivity is to maintain the intestinal health of the sows. Reasonable nutrition regulation measures are taken to improve the feed intake of the sows in the later gestation period and the lactation period and improve the constipation condition in the perinatal period, so that the production performance of the sows is improved, and the problem to be solved in the modern pig raising industry is urgent.
The establishment and maintenance of the intestinal microbial flora play an important role in the health of animal intestinal tracts, the reproductive performance of sows can be improved, the weight of weaned piglets is improved, the feed conversion rate is improved, the intestinal peristalsis performance is promoted, the constipation of sows is reduced, the excessive fermentation and proliferation of harmful microbial flora in the rear intestinal tracts are controlled, the healthy fermentation of beneficial microbial flora in the intestinal tracts is promoted, the bad fermentation of the rear intestinal tracts is reduced, the concentration and the odor of excrement and ammonia and hydrogen sulfide in a breeding house are reduced, the environment in the house is improved, the pollution is reduced, and the health and sustainable development of the breeding industry is promoted.
In recent years, microbial preparations and enzyme preparations as safe novel feed additives have become hot research of scholars at home and abroad. The bacillus in the microbial strains can secrete digestive enzymes such as protease and amylase, so that the intestinal nutrient absorption of animals is promoted, and the utilization rate of the feed is improved. Meanwhile, because spores can be formed, the microbial feed additive has the stress resistance of high temperature resistance, acid and alkali resistance and the like, is convenient to produce and store, and is one of the most commonly used microbial feed additive strains at present. The lactobacillus can be fixedly planted and rapidly proliferated in the intestinal tracts of animals to generate organic acid, so that the growth of pathogenic microorganisms can be effectively inhibited, and the intestinal flora balance can be maintained. The mannase in the enzyme preparation can decompose mannan in feed raw materials such as soybean meal and the like, eliminate the anti-nutritional effect of the mannase, reduce the viscosity of intestinal chyme and promote intestinal digestion and absorption. In addition, the mannan oligosaccharide generated by enzymolysis reaches the cecum through the intestinal tract, so that food can be provided for the propagation of beneficial microorganisms such as lactobacillus and bifidobacterium, the number of the beneficial microorganisms is increased, the growth of pathogenic bacteria is inhibited, and the intestinal health of animals is improved. According to the invention, bacillus and lactic acid bacteria in the microbial preparation are scientifically compatible with mannase in the enzyme preparation, so that the intestinal regulation function of probiotics is fully exerted, and the intestinal health of sows is maintained.
Disclosure of Invention
The invention aims to provide a compound biological preparation prepared by compounding bacillus licheniformis, enterococcus faecium, lactobacillus plantarum and mannase, which is prepared by scientifically matching beneficial microbial strains with specific functions with the mannase, maintains the intestinal health of sows and solves the problems of low feed intake, constipation and the like of the sows in animal production.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
a compound biological preparation for regulating intestinal health of sow mainly comprises effective components such as Bacillus licheniformis (with preservation number of CGMCC NO.14441), enterococcus faecium (with preservation number of CGMCC NO.9666), Lactobacillus plantarum (with preservation number of CGMCC NO.14459), and mannanase.
Preferably, the viable count of the bacillus licheniformis in the composite biological preparation is 1.0 multiplied by 108-1.0×1010CFU/g。
Preferably, the viable count of the enterococcus faecium in the composite biological preparation is 1.0 multiplied by 108-1.0×1010CFU/g。
Preferably, the viable count of the lactobacillus plantarum in the composite biological preparation is 1.0 multiplied by 108-1.0×1010CFU/g。
Preferably, the enzyme activity of the mannanase in the compound biological agent is 1000-10000U/g.
A preparation method of a compound biological agent for regulating the intestinal health of sows comprises the following steps: mixing the effective components of Bacillus licheniformis, enterococcus faecium, Lactobacillus plantarum, mannanase, etc. with the carrier such as zeolite powder, etc. according to different addition amounts.
The application of the compound biological preparation for regulating the intestinal health of the sows in the feed comprises the steps of adding the compound biological preparation for regulating the intestinal health of the sows into a daily ration feed according to the addition amount of 0.05-0.2% of the weight of the feed, uniformly mixing and feeding.
The bacillus licheniformis with high yield of neutral protease and amylase can effectively improve the feed intake of sows in the breeding period and improve the utilization rate of feed. Enterococcus faecium and lactobacillus plantarum can quickly proliferate to generate organic acid, and effectively inhibit the growth of harmful microorganisms. The mannase can decompose mannan in the feed to generate mannan oligosaccharide, and the mannan oligosaccharide can promote proliferation of probiotics in animal intestinal tracts, so that the health of the animal intestinal tracts is regulated, and the constipation rate of sows is reduced.
According to the invention, beneficial microorganisms with specific functions and mannase preparations are scientifically combined and synergistically applied, the regulation effect of the beneficial microorganisms on animal intestinal tracts can be fully exerted, the feed intake of sows can be effectively improved, the feed utilization rate is improved, the growth of harmful microorganisms is inhibited, the proliferation of probiotics in the intestinal tracts is promoted, the constipation rate of sows is reduced, and the intestinal health of the sows is maintained.
Drawings
FIG. 1 is a culture colony screened for protease-producing strains;
FIG. 2 shows a selected culture colony of an amylase-producing strain.
Detailed Description
The technical solution of the present invention is further described below with reference to examples:
EXAMPLE 1 screening and identification of protease-producing, Amylase-producing Bacillus strains
Test method 1
1.1 screening of protease-producing strains
Primary screening: the isolated and purified strain was spotted on a casein medium plate by the casein plate hydrolysis cuvette method, cultured in a thermostat for 48 hours, colonies that produced hydrolysis cuvette were picked up (as shown in fig. 1), and the L value (L value ═ cuvette diameter/colony diameter) was measured and stored at 4 ℃ for further use.
Re-screening: the bacterial strain producing the transparent circle is obtained by primary screening, and 1mL of bacterial suspension (10) is absorbed8CFU/mL) in a fermentation medium, uniformly mixing, naturally adjusting pH value, and culturing in an incubator at 37 ℃ for 24h to determine protease activity.
And (3) enzyme activity determination: taking the enzyme solution to be detected. And (3) carrying out constant-temperature water bath at 40 ℃ for 2min, adding 2mL (shaking up) of casein, carrying out constant-temperature water bath at 40 ℃ for 10min, then adding 4mL (shaking up) of trichloroacetic acid, centrifuging to obtain a supernatant, and measuring the light absorption value at 275 nm. At the same time, blank control was performed.
1.2 α -screening of Amylase-producing strains
Primary screening: the strain is inoculated on a sterilized starch agar culture medium, is statically cultured for 24 hours at 37 ℃, is transferred into a liquid triangular flask filled with 25mL of the culture medium, and is cultured for 24 hours at 37 ℃ and 220r/min in a shaking way. Diluting 1mL culture solution with sterile water, respectively spreading 0.15mL bacterial suspension on plate separation culture medium, culturing at 37 deg.C for 24h, spraying iodine solution on the plate, picking out single colony with transparent ring (as shown in FIG. 2), streaking, separating, picking to slant preservation culture medium, culturing at 37 deg.C for 24h, and storing at 4 deg.C.
And (3) secondary screening, namely taking 1-ring primary screening strain to be inoculated into a seed culture medium, carrying out shake culture at 37 ℃ and 220r/min for 24h, taking 1mL of strain to be inoculated into a solid enzyme production culture medium, carrying out constant temperature culture at 30 ℃ for 72h, then measuring α -amylase activity, and storing the strain with the best activity at 4 ℃.
The method for measuring the enzyme activity comprises the following steps: preheating substrate 5mL in 40 deg.C water bath for 10min, adding enzyme solution 0.5mL, maintaining temperature for 5min, adding mixed solution 0.5mL into H5 mL with 0.1mol/L2SO4The reaction was terminated, 0.5mL of the resulting solution was added to 5mL of a dilute iodine solution to develop color, and the absorbance at 660nm was measured. 0.5mL of buffer was used as a control in place of 0.5mL of enzyme solution, and distilled water was used as a blank for color comparison.
2 results of the experiment
2.1 preliminary screening
The casein circle-producing strain was initially screened from 30 strains of bacteria for 15 strains, which accounted for 50% of all tested strains. The L value was initially determined (see Table 1) and the data shows that the L value was maximal for strain Y27, at 3.01.
TABLE 1 Primary screening of protease producing strains
2.2 rescreening and enzyme Activity determination
5 strains with larger L value are selected, and the protease activity of the strains is measured after fermentation culture (see table 2), wherein in the 5 strains, the strain with larger L value and enzyme activity is Y27, the L value is 3.01, and the protease activity is 324.72U/mL.
TABLE 2 rescreening of the strains
2.3 α -screening of Amylase-producing strains
The amylase production experiment shows that 9 strains in total produce α -amylase and account for 30% of the tested strains, further re-screening shows that Y8, Y20 and Y27 produce α -amylase with high enzyme production, wherein Y27 strain produces high enzyme and the enzyme activity reaches 225.76U/g, Y30 strain with low enzyme activity and 67.9U/g are used as the enzyme production strains, and the results are shown in Table 3.
TABLE 3 α results of amylase activity assay
2.4 identification of the strains
The similarity of the gene sequence (SEQ ID NO.1) of the Y27 strain to Bacillus licheniformis (Bacillus licheniformis) was 99% as identified by the gyrB gene sequence, and it was identified as Bacillus licheniformis (Bacillus licheniformis). The strain is preserved in China general microbiological culture Collection center (CGMCC for short, the address is No. 3 of the institute of microbiology of the national academy of sciences of China, No. 100101 of the national institute of microbiology, North Chen West Lu No.1 of the south-rising area of Beijing) in 2017, 19 months, and the preservation number is CGMCC NO. 14441.
Example 2 Effect of mannooligosaccharides on growth of enterococcus faecium and Lactobacillus plantarum
1 materials and methods
1.1 test materials
1.1.1 Strain
Enterococcus faecium (the strain is preserved in China general microbiological culture Collection center (CGMCC, address: No.1 Sihui way No. 3 of the sunward area in Beijing, China academy of sciences, postal code 100101) in 9-16 th 2014, the preservation number is CGMCC NO.9666), and Lactobacillus plantarum (the strain is preserved in China general microbiological culture Collection center (CGMCC, address: No. 3 of the sunward area in Beijing, China academy of sciences, postal code 100101) in 7-25 th 2017 th, the preservation number is CGMCC NO. 14459).
1.1.2 culture Medium
(1) Seed activation Medium (MRS Medium)
10g of peptone, 5g of beef extract, 5g of yeast extract, 20g of glucose, Tween-801 mL, 2g of dipotassium phosphate, 5g of sodium acetate, 2g of diammonium citrate, 0.2g of magnesium sulfate, 0.05g of manganese sulfate and distilled water (ddH)2O)1000mL, 121 ℃, and sterilizing for 30 min.
(2) Carbon source-free culture medium
10g of peptone, 5g of yeast extract, 801 mL of Tween-801, 2g of dipotassium phosphate, 5g of sodium acetate, 2g of diammonium citrate, 0.1g of magnesium sulfate, 0.05g of manganese sulfate and distilled water (ddH)2O)1000mL, 121 ℃, and sterilizing for 30 min.
(3) Fermentation medium
On the basis of a carbon source-free culture medium, different amounts of mannooligosaccharides are added, glucose treatment with the concentration of 2% is taken as a control, and in order to avoid high-temperature loss of glucose and mannooligosaccharides, a 0.22 mu m filter is adopted for filtration sterilization.
1.1.3 sterile Water and other materials
Sterile water (9mL), 1mL pipette, petri dish and the like and autoclaved at 121 ℃ for 30 min.
1.2 test methods
1.2.1 seed liquid activation
Respectively selecting enterococcus faecium and Lactobacillus plantarum lawn by using an inoculating loop, inoculating the enterococcus faecium and the Lactobacillus plantarum lawn into a seed activation culture medium, and standing and culturing at 37 ℃ for 18 h.
1.2.2 inoculation
1mL of enterococcus faecium and Lactobacillus plantarum seed liquid (1% volume ratio) is respectively sucked and inoculated into culture media taking glucose and mannooligosaccharides with different concentrations as carbon sources.
1.2.3 cultivation
And standing and culturing for 18h at 37 ℃.
1.2.4 viable count
(1) Accurately sucking 1mL of zymogen liquid to be detected, transferring the zymogen liquid to a test tube filled with 9mL of sterile water, vibrating for 15s, and uniformly mixing the zymogen liquid to obtain 10-1Diluting the solution; by analogy, continuously diluting to obtain 10-6、10-7And (5) diluting the bacterial liquid.
(2) Mixing plate
Numbering the sterile culture dishes, setting three groups of parallel bacteria liquid in each dilution, accurately sucking 1mL of bacteria liquid with a certain dilution by using a sterile pipette strictly according to the sterile operation requirement, and respectively putting the bacteria liquid into the three groups of parallel culture dishes. Then, nutrient agar culture medium which is melted and cooled to about 50 ℃ is poured into the culture dish. The plate was gently rotated 100 times to mix the broth with the medium.
(3) Culturing
After the mixed plate culture medium is condensed, the mixed plate culture medium is inverted and cultured in an incubator at 37 ℃ for 48 hours.
(4) Counting
Calculating the formula: the number of bacteria per mL of fermentation broth is the average number of colonies repeated several times at the same dilution x dilution.
2 test results and analysis
2.1 Effect of mannooligosaccharides on the growth of enterococcus faecium
As can be seen from Table 4, the viable count of enterococcus faecium in the fermentation broth increases with the addition of mannooligosaccharide, and when the addition is more than 1%, the increase is reduced. In the treatment of adding the mannose oligosaccharide with different concentrations as the carbon source, the number of the viable bacteria of the enterococcus faecium in the fermentation activation liquid is higher than that in the treatment of taking 2 percent of glucose as the carbon source, which shows that the mannose oligosaccharide has the promotion effect on the growth of the enterococcus faecium.
TABLE 4 Effect of mannooligosaccharides on the growth of enterococcus faecium
2.2 Effect of mannooligosaccharides on growth of Lactobacillus plantarum
As can be seen from Table 5, the viable count of Lactobacillus plantarum in the fermentation broth increases with the addition of mannooligosaccharides, and when the addition is greater than 1%, the increase is smaller. In the treatment of adding different concentrations of the mannooligosaccharides as the carbon source, the number of viable bacteria of the lactobacillus plantarum in the fermentation activating solution is higher than that in the treatment of taking 2% of glucose as the carbon source, which shows that the mannooligosaccharides have a promoting effect on the growth of the lactobacillus plantarum.
TABLE 5 Effect of mannooligosaccharides on Lactobacillus plantarum growth
3 conclusion
Compared with different types of carbon sources, the viable count of the fermentation liquor of the enterococcus faecium and the lactobacillus plantarum taking the mannooligosaccharides as the carbon source is higher than that of the fermentation liquor of the enterococcus faecium and the lactobacillus plantarum taking 2 percent of glucose as the carbon source. The results show that the mannooligosaccharides can promote the growth of enterococcus faecium and lactobacillus plantarum.
Example 3 preparation of Complex biological preparation for regulating intestinal health of sow
The production method of the compound biological preparation comprises the following steps:
1. production of Bacillus licheniformis
(1) Inoculating Bacillus licheniformis strain on slant culture medium, and culturing at 30-40 deg.C for 12-24 hr.
(2) Inoculating the slant cultured Bacillus licheniformis colony in seed culture medium with liquid loading amount of 50-150mL/500mL, and culturing at 30-40 deg.C and 120-.
(3) Inoculating the Bacillus licheniformis seed solution in a seed tank at 0.1-0.5 wt%, loading liquid 50-150L/500L, and culturing at 30-40 deg.C and 180 rpm for 12-24 hr.
(4) Inoculating the seeding tank culture solution into a fermentation tank according to the weight ratio of 0.1-0.5%, wherein the tank pressure is 0.01-0.05MPa, the stirring speed is 120-: 0.1-0.5 (volume ratio), and the fermentation time is 12-24 hours.
(5) Drying by adopting spray drying equipment, wherein the inlet air temperature is 140-160 ℃, the outlet temperature is 60-70 ℃, and the final material moisture is 6-10% (weight ratio), so as to obtain the bacillus licheniformis spray dry powder.
The slant culture medium in the step (1) comprises the following components: 1.0-5.0g of beef extract, 5.0-15.0g of soybean peptone, 1.0-5.0g of yeast extract, 1.0-5.0g of sodium chloride, 1.0-10.0g of glucose, 15-20g of agar, 1000mL of distilled water and pH of 7.0;
the seed culture medium in the step (2) comprises: 5-15g of peptone, 0.1-2g of beef extract, 0.1-3g of sodium chloride and 1000mL of distilled water, wherein the pH value is 7.4;
the culture medium of the seeding tank in the step (3) comprises the following components: corn flour 0.1-0.5%, glucose 0.1-1.0%, bean cake powder 0.1-3%, fish meal 0.01-0.1%, calcium carbonate 0.1-2%, ammonium sulfate 0.01-0.05%, dipotassium hydrogen phosphate 0.001-0.01%, magnesium sulfate 0.001-0.01% and manganese sulfate 0.001-0.01%;
the fermentation medium in the step (4) comprises the following components: 0.1 to 3 percent of yeast powder, 0.1 to 3 percent of soybean meal hydrolysate, 0.1 to 1 percent of glucose, 0.1 to 1.5 percent of corn starch, 0.1 to 0.5 percent of calcium carbonate and the pH value of 7.0 to 7.5.
2. Production of enterococcus faecium and lactobacillus plantarum
(1) Inoculating enterococcus faecium or Lactobacillus plantarum in MRS solid slant culture medium, and culturing at 30-40 deg.C under aerobic or facultative condition for 12-24 hr.
(2) Inoculating enterococcus faecium cultured on slant into seed culture medium, and culturing at 30-40 deg.C under aerobic or facultative condition for 12-24 hr.
(3) Inoculating the seed liquid obtained in the step (2) into a fermentation tank, and culturing for 12-24 hours at 30-40 ℃ under aerobic or facultative conditions.
(4) And (4) centrifuging the enterococcus faecium or lactobacillus plantarum zymocyte liquid obtained in the step (3) to collect thalli, wherein the centrifugal recovery yield is more than 85%.
(5) Weighing the bacterial sludge centrifuged in the step (4), uniformly mixing the bacterial sludge and a protective agent according to the volume-to-mass ratio of 5:1, pre-freezing at-70 ℃ for 2-3h, starting a freeze dryer in advance, quickly freezing the pre-frozen thallus for 8h when the freezing temperature is reduced to-40 ℃, and obtaining the freeze-dried powder of the enterococcus faecium or the lactobacillus plantarum, wherein the survival rate of the enterococcus faecium is 90-95% after freeze-drying.
Wherein the formula of the protective agent is as follows: 14% of skimmed milk powder, 10% of sucrose, 16% of fructo-oligosaccharide, 10% of sodium glutamate and 1L of sterile water.
The MRS slant culture medium in the step (1) comprises: 10g of peptone, 5g of beef extract, 5g of yeast extract, 20g of glucose, 801 mL of tween-801, 2g of dipotassium phosphate, 5g of sodium acetate, 2g of diammonium citrate, 0.2g of magnesium sulfate, 0.05g of manganese sulfate, 6g of calcium carbonate, 16g of agar powder, 1000mL of distilled water, 121 ℃ and sterilizing for 30 min. And (3) the seed culture medium in the step (2) is the MRS slant culture medium without agar powder. The improved MRS culture medium in the step (3) comprises the following components: 5-30g of soybean peptone, 1-10g of glucose, 1-10g of yeast powder, 1-10g of sodium acetate, 0.1-8g of diamine citrate, 800.1-5 g of Tween, 0.1-5g of dipotassium hydrogen phosphate, 0.05-lg of magnesium sulfate, 0.001-0.2 g of manganese sulfate, 1-30g of calcium carbonate and 1000mL of distilled water, and the pH value is adjusted to be 5.5-7.5.
3. The production steps of the mannase are as follows:
the solid mannase preparation is obtained by inoculating and culturing the first-stage seeds, inoculating and culturing the second-stage seeds, fermenting, flocculating, filter-pressing, ultrafiltering, finely filtering, spray-drying and screening the enzyme-producing strains.
4. According to the viable count of the bacillus licheniformis in the composite biological preparation of 1.0 multiplied by 108-1.0×1010CFU/g, viable count of enterococcus faecium 1.0 × 108-1.0×1010CFU/g, viable count of Lactobacillus plantarum 1.0 × 108-1.0×1010CFU/g, the enzyme activity of the mannase is 1000-10000U/g, and the composite biological preparation for regulating the intestinal health of the sow can be prepared by uniformly mixing the products of bacillus licheniformis, enterococcus faecium, lactobacillus plantarum and mannase with carriers such as zeolite powder and the like.
Example 4 Effect of Complex biologics on sow Productivity, stool morphology and stool microorganism reproduction
1 materials and methods
1.1 test animals and groups
120 long x large binary pregnant sows with close mating time, approximately same gestation times, close weight and good health condition and 60 days of gestation are selected and randomly divided into four groups in the test. The control group was fed with basal diet, test group I added 0.5 kg/ton of the composite biological agent to the basal diet, test group II added 1 kg/ton of the composite biological agent to the basal diet, and test group III added 2 kg/ton of the composite biological agent to the basal diet, each group containing 30 sows. The feeding conditions of two groups of test sows and piglets produced by the test sows are ensured to be consistent in the test process, the sows are fed with daily ration in the gestation period from the beginning of the test to the 90 th day of the pregnancy, and are fed with daily ration in the lactation period from the 90 th day of the pregnancy to the end of the test, and the test time is 60 days of the pregnancy to 28 days of weaning.
1.2 Breeding management
Feeding management is carried out according to a conventional method of a pig farm, pregnant pigs are fed in a limiting fence, and the pregnant pigs are fed for 2 times (08: 00 and l 6: 30) every day, and are freely drunk after being basically eaten. The test sows migrated to the farrowing house 1 week prior to parturition, and the sows feed freely during lactation.
1.3 measurement index and method
1.3.1 sow production performance index determination
During the whole experiment period, the health conditions of the sows of the control group and the test group and the born piglets are observed, the average daily feed intake of the sows of 2 groups is respectively recorded, and litter size, average birth weight, average litter weight of weaned piglets and the like are measured.
1.3.2 sow feces morphology and feces microorganism index determination
Sow stool morphology was observed and scored according to stool morphology scoring criteria (see table 6).
TABLE 6 stool morphology scoring criteria
On the day of test completion, 8 lactating sows are randomly selected respectively, fresh feces of the lactating sows are collected and stored in a refrigeration box, and the viable count of lactic acid bacteria and coliform bacteria in the feces is measured respectively.
2 results of the test
2.1 Effect of Complex biologics on sow Productivity
As can be seen from Table 7, the average daily food intake of the sows in the test group is significantly higher than that of the control group, and the average daily food intake is improved by over 280 g. The litter size and the average birth weight of the weaned piglets in the test group are not obviously different from those in the control group, and the average weight of the weaned piglets in the test group is increased by more than 90g compared with that in the control group, and is obviously higher than that in the control group. The result shows that the feed intake and the production performance of the sows can be improved by adding the composite biological agent, and the average weight of the weaned piglets is improved.
TABLE 7 Effect of Complex biologics on sow Productivity
Note: the data in the same row followed by different letters indicates significant difference (P <0.05), and the same letters indicate insignificant difference (P > 0.05). Results are expressed as mean ± standard deviation, as follows.
2.2 Effect of Complex biologicals on sow fecal morphology and fecal microbiology
As can be seen from Table 8, the differences between the test group and the control group are significant, and the constipation rate of the sows in the test group is reduced by more than 10 percent compared with that in the control group (p is less than 0.05). In addition, the content of lactic acid bacteria in the feces of the sows in the test group is obviously higher than that of the control group, and the content of coliform in the feces of the sows in the test group is obviously lower than that of the control group, so that the content is reduced by more than 4.74 percent. The test result shows that the compound biological agent can obviously improve the intestinal flora of the sows, increase the quantity of beneficial bacteria, reduce the quantity of harmful bacteria and reduce the constipation rate of the sows when being fed.
TABLE 8 Effect of Complex biologics on sow fecal morphology and fecal microbiology
3 conclusion
The compound biological agent with the content of 0.05-0.2 per mill is added into the daily ration of the sow, so that the production performance of the sow can be obviously improved, the intestinal flora of the sow can be obviously improved, the quantity of beneficial bacteria can be increased, the quantity of harmful bacteria can be reduced, and the constipation rate of the sow can be reduced.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and are not limited. Although the present invention has been described in detail with reference to the embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims.
Sequence listing
<110> Beijing Kogyo Bo Biotech Co., Ltd
<120> composite biological preparation for regulating intestinal health of sows, and preparation method and application thereof
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gcaaaattgc ttggaaatga cggtcgcggc cctgttttgc cgatccgccc gcagagtcac 180
cctcaacgat gtaaagttcg gaaatcgtcg ggtctttaga agaacagtca gcaagtttcc 240
ccggcagatt ggacacttca agggcgcttt ttctgcgcgt caattcgcgt gctttctttg 300
cagccatccg tgctctggcg gccataaccc ctttttcaac gatttttttc gctgaatccg 360
ggttttctag cagaaacttt tcaagcgctt ctgaaaatag cgcatctgtt atcgtacgcg 420
cttctgagtt gccgagcttt gttttcgtct gcccttcaaa ttgaggatcc gggtgcttga 480
ttgaaataat cgctgtcaaa ccttcccgga cgtcttctcc gcttaagttc ggatcgcttt 540
ctttgaatac gccgtttctt ctcgcgtaat cattgatgac cctcgtcaaa ccggtcttaa 600
agccggcttc atgggttccg ccttcatacg tatgaatgtt gttagcaaat gaataaatgt 660
tgcttgtata gctgtcattg tattgaagag ccacctcgac tgtaatgccg tctttggatc 720
cttcaatata gaccggctct tcatgaataa cttcccgcga acggttcaag tgttcaacat 780
agcttttaat accgccttca tagcagtatt cattcttgcg ttcttttcct tctcgcttgt 840
cttcgatcgt gattttgacg ccttttgtca agaaagcgag ttcgcggaca cgagtggcga 900
gcgtatcata gtcgtattca gtcgtttccg tgaatatttc cggatcaggc ttgaagtgtg 960
tggtcgttcc cgtcacttcc gtatctccaa tgactttcaa atcagctttc ggaacgccac 1020
gttcaaattc ctgataatgg atttttccat ctctgtaaac cgttacatcc agctcggttg 1080
aaagggcgtt aacatcagaa gcaccgacgc cgt 1113
Claims (6)
1. The compound biological preparation for regulating the intestinal health of the sows is characterized by being mainly prepared by compounding bacillus licheniformis, enterococcus faecium, lactobacillus plantarum and mannanase.
2. The composite biological agent for regulating the intestinal health of sows as claimed in claim 1, wherein the viable count of Bacillus licheniformis is 1.0 x 108-1.0×1010CFU/g, viable count of enterococcus faecium is 1.0 × 108-1.0×1010CFU/g, viable count of Lactobacillus plantarum 1.0 × 108-1.0×1010CFU/g, the enzyme activity of the mannase is 1000-10000U/g.
3. The method for preparing a complex biological agent for regulating the intestinal health of sows as claimed in claim 1, which comprises the following steps:
mixing Bacillus licheniformis, enterococcus faecium, Lactobacillus plantarum and mannanase with carrier.
4. The method according to claim 3, wherein the carrier is zeolite powder.
5. The use of the complex biological agent for regulating the intestinal health of sows as set forth in claim 1 in feed.
6. The application according to claim 5, wherein the application is:
adding the compound biological agent for regulating the intestinal health of the sows into the daily feed according to the addition amount of 0.05-0.2% of the weight of the daily feed, and feeding after uniformly mixing.
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Cited By (1)
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Application publication date: 20200508 |