CN111060493A - Surface-enhanced Raman spectrum multi-sample detection system - Google Patents
Surface-enhanced Raman spectrum multi-sample detection system Download PDFInfo
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- CN111060493A CN111060493A CN201911383821.7A CN201911383821A CN111060493A CN 111060493 A CN111060493 A CN 111060493A CN 201911383821 A CN201911383821 A CN 201911383821A CN 111060493 A CN111060493 A CN 111060493A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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Abstract
The invention discloses a surface-enhanced Raman spectrum multi-sample detection system, which comprises: the base, X axle drive assembly, Y axle drive assembly, mounting bracket, detect dish, raman detector, X axle drive assembly and Y axle drive assembly drive detection dish remove, through carrying out optimal design to X axle drive assembly and Y axle drive assembly, guarantee to detect that dish shift position is accurate to realize detecting a plurality of samples on the dish and accurately detect in proper order, improve detection efficiency and detection precision greatly.
Description
Technical Field
The invention relates to the field of surface enhanced Raman spectroscopy, in particular to a surface enhanced Raman spectroscopy multi-sample detection system.
Background
In recent years, raman spectroscopy has become an important tool for non-contact, on-site and non-destructive testing, which can directly test a substance, and has the characteristics of rapidness, simplicity, non-destruction and directness, and thus raman spectroscopy is favored in the field of on-site testing. However, in many application fields, the low detection sensitivity becomes a defect of the conventional Raman spectroscopy technology.
At present, the ultra-sensitive trace detection characteristic of SERS (surface enhanced Raman spectroscopy) has a great progress in the field of trace detection. The surface enhanced Raman spectroscopy technology adsorbs target analytes on a surface enhanced plane made of silver, copper or gold and the like, and excites a light source with specific wavelength to cause surface absorption band or surface matrix resonance, so that Raman spectrum signals of the target analytes in an action region are enhanced 104～106With the successful development and application of the surface enhanced Raman scattering technology, the problems of low sensitivity and large fluorescence interference in the conventional Raman spectrum detection technology are solved. As the technology for preparing the uniform and stable SERS nano-substrate with high activity is continuously mature and developed, and the surface enhanced Raman spectroscopy technology is combined, the rapid trace detection of a biochemical site is finally realized. In the process of Raman detection, the influence of the laser of an instrument and the focal length of a substrate on the detection sensitivity of a substance is large, a portable Raman spectrometer is generally adopted at present, the focal length is adjusted manually, but a better focus point can be found through multiple times of adjustment, and the detection time is influenced. The surface-enhanced Raman spectrum multi-sample detection system is the best focal length, and the process of manual adjustment is reduced, so that the system is very important.
Disclosure of Invention
In order to solve the technical problems in the background technology, the invention provides a surface-enhanced Raman spectrum multi-sample detection system.
The invention provides a surface-enhanced Raman spectrum multi-sample detection system, which comprises: the device comprises a base, an X-axis driving assembly, a Y-axis driving assembly, a mounting frame, a detection disc and a Raman detector;
the X-axis driving assembly is positioned on the base and comprises a first support, a first sliding rail, a first stepping motor and a first spiral guide rod, the first support is installed on the base, the first sliding rail is horizontally arranged on the base, a first sliding table capable of being installed in a sliding mode is arranged on the first sliding rail, a first screw hole extending in a direction parallel to the first sliding rail is formed in the first sliding table, the first spiral guide rod is rotatably installed on the first support and is in threaded fit with the first screw hole, the first stepping motor is positioned on one side of the first support, and a driving shaft of the first stepping motor is coaxially arranged with the first spiral guide rod and is connected with the first spiral guide rod;
the Y-axis driving assembly is arranged on the first sliding table, the detection disc is arranged on the Y-axis driving assembly, the Y-axis driving assembly is used for driving the detection disc to move in a direction perpendicular to the first sliding rail, and a plurality of sample detection positions are arranged on the detection disc;
the mounting bracket is installed on the base and is located detection dish one side, and the raman detector is installed on the mounting bracket and is located detection dish top and towards the detection dish setting.
Preferably, Y axle drive assembly includes second support, second slide rail, second step motor and second spiral guide arm, and the second support mounting is on first slip table, and the first slide rail level of second slide rail perpendicular to sets up on first slip table, is equipped with slidable mounting's second slip table on the second slide rail, is equipped with the second screw that is on a parallel with the extension of second slide rail direction on the second slip table, second spiral guide arm rotatable install on the second support and with second screw thread fit, second step motor is located second support one side, the drive shaft of second step motor and second spiral guide arm coaxial arrangement and be connected with the spiral guide arm, detect the dish and install on the second slip table.
Preferably, the detection device further comprises an air pump, a negative pressure cavity is arranged in the detection disc, an air suction hole is formed in the top of the negative pressure cavity, an air outlet hole is formed in the side wall of the negative pressure cavity, and the air pump is connected with the air outlet hole through an air pipe.
Preferably, the detection plate comprises a detection seat and a sample table, wherein the top of the detection seat is provided with a holding groove for holding the sample table, and the air suction hole is located in the holding groove.
Preferably, the receiving groove extends from one end to a middle portion at a top of the test socket.
Preferably, the stepping device further comprises a control system, and the control system is connected with the first stepping motor and/or the second stepping motor and is used for controlling the first stepping motor and/or the second stepping motor to work.
In the invention, the proposed surface enhanced Raman spectroscopy multi-sample detection system comprises: the base, X axle drive assembly, Y axle drive assembly, mounting bracket, detect dish, raman detector, X axle drive assembly and Y axle drive assembly drive detection dish remove, through carrying out optimal design to X axle drive assembly and Y axle drive assembly, guarantee to detect that dish shift position is accurate to realize detecting a plurality of samples on the dish and accurately detect in proper order, improve detection efficiency and detection precision greatly.
Drawings
Fig. 1 is a schematic structural diagram of a surface-enhanced raman spectroscopy multi-sample detection system according to the present invention.
Fig. 2 is another schematic structural diagram of a surface-enhanced raman spectroscopy multi-sample detection system according to the present invention.
Fig. 3 is a schematic structural view of the detection disk.
Detailed Description
As shown in fig. 1 to 3, fig. 1 is a schematic structural diagram of a surface-enhanced raman spectroscopy multi-sample detection system according to the present invention, fig. 2 is another schematic structural diagram of the surface-enhanced raman spectroscopy multi-sample detection system according to the present invention, and fig. 3 is a schematic structural diagram of a detection tray.
Referring to fig. 1 to 2, the present invention provides a surface-enhanced raman spectroscopy multi-sample detection system, including: the device comprises a base 1, an X-axis driving assembly, a Y-axis driving assembly, a mounting frame 5, a detection disc 4 and a Raman detector 7;
the X-axis driving assembly is positioned on the base 1 and comprises a first support 21, a first sliding rail, a first stepping motor 22 and a first spiral guide rod 23, the first support 21 is installed on the base 1, the first sliding rail is horizontally arranged on the base 1, the first sliding rail is provided with a first sliding table 24 which can be installed in a sliding mode, the first sliding table 24 is provided with a first screw hole extending in a direction parallel to the first sliding rail, the first spiral guide rod 23 is rotatably installed on the first support 21 and is in threaded fit with the first screw hole, the first stepping motor 22 is positioned on one side of the first support 21, and a driving shaft of the first stepping motor 22 is coaxially arranged with the first spiral guide rod 23 and is connected with the first spiral guide rod;
the Y-axis driving assembly is arranged on the first sliding table 24, the detection disc 4 is arranged on the Y-axis driving assembly, the Y-axis driving assembly is used for driving the detection disc 4 to move in the direction perpendicular to the first sliding rail, and a plurality of sample detection positions are arranged on the detection disc 4;
mounting bracket 5 installs on base 1 and is located detection dish 4 one side, and raman detector 7 installs on mounting bracket 5 and is located detection dish 4 top and towards detection dish 4 setting.
In the specific working process of the surface-enhanced Raman spectrum multi-sample detection system, the X-axis driving assembly is used for driving the Y-axis driving assembly to move along the X-axis direction, and the Y-axis driving assembly is used for driving the detection disc to move along the Y-axis direction, so that samples to be detected on the detection disc sequentially move into a detection area of the Raman detector, and the Raman detector detects a plurality of samples on the detection disc one by one.
In the specific driving mode of the X-axis direction of the X-axis driving assembly, the first stepping motor drives the first spiral guide rod to rotate, the first spiral guide rod rotates to drive the first sliding table to move along the first sliding rail through thread matching, and therefore the detection disc is further driven to move along the X direction.
In this embodiment, the proposed surface-enhanced raman spectroscopy multi-sample detection system includes: the base, X axle drive assembly, Y axle drive assembly, mounting bracket, detect dish, raman detector, X axle drive assembly and Y axle drive assembly drive detection dish remove, through carrying out optimal design to X axle drive assembly and Y axle drive assembly, guarantee to detect that dish shift position is accurate to realize detecting a plurality of samples on the dish and accurately detect in proper order, improve detection efficiency and detection precision greatly.
In a specific embodiment of the Y-axis driving assembly, the Y-axis driving assembly includes a second bracket 31, a second slide rail, a second stepping motor 32 and a second spiral guide rod 33, the second bracket 31 is installed on the first sliding table 24, the second slide rail is horizontally disposed on the first sliding table 24 perpendicular to the first slide rail, the second slide rail is provided with a second sliding table 34 capable of being installed in a sliding manner, the second sliding table 34 is provided with a second screw hole extending in parallel to the direction of the second slide rail, the second spiral guide rod 33 is rotatably installed on the second bracket 31 and is in threaded fit with the second screw hole, the second stepping motor 32 is located on one side of the second bracket 31, a driving shaft of the second stepping motor 32 is coaxially arranged with the second spiral guide rod 33 and is connected with the spiral guide rod, and the detection disc 4 is installed on the second sliding table 34; in the specific driving mode of the Y-axis direction of the Y-axis driving assembly, the second stepping motor drives the second spiral guide rod to rotate, the second spiral guide rod rotates to drive the second sliding table to move along the second sliding rail through thread matching, and therefore the detection disc is driven to move along the Y direction in a further two-step mode.
Referring to fig. 3, when a sample is placed on a detection disc, the problem of untight fit is easily caused, the embodiment further comprises an air pump 6, a negative pressure cavity is arranged in the detection disc 4, an air suction hole is formed in the top of the negative pressure cavity, an air outlet hole is formed in the side wall of the negative pressure cavity, and the air pump 6 is connected with the air outlet hole through an air pipe; negative pressure is formed in the negative pressure cavity inside the detection disc through the air pump, so that the sample disc and the detection disc are attached more tightly, better precision is guaranteed, and the accuracy of a detection result is improved.
In further concrete design, detect dish 4 including detecting seat 41 and sample platform 42, detect the seat 41 top and be equipped with the holding tank that is used for holding sample platform 42, the aspirating hole is located in the holding tank, the sample dish is placed in the holding tank that detects the dish, improves the cooperation precision.
To facilitate the mounting of the sample plate, further, the receiving groove extends from one end to the middle at the top of the test seat 41.
In order to realize the automatic Raman detection of multiple samples, the system also comprises a control system which is connected with the first stepping motor 22 and/or the second stepping motor 32 and is used for controlling the first stepping motor 22 and/or the second stepping motor 32 to work.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. A surface enhanced raman spectroscopy multi-sample detection system, comprising: the Raman detector comprises a base (1), an X-axis driving assembly, a Y-axis driving assembly, a mounting frame (5), a detection disc (4) and a Raman detector (7);
the X-axis driving assembly is positioned on the base (1) and comprises a first support (21), a first sliding rail, a first stepping motor (22) and a first spiral guide rod (23), the first support (21) is installed on the base (1), the first sliding rail is horizontally arranged on the base (1), a first sliding table (24) capable of being installed in a sliding mode is arranged on the first sliding rail, a first screw hole extending in a direction parallel to the first sliding rail is formed in the first sliding table (24), the first spiral guide rod (23) is rotatably installed on the first support (21) and is in threaded fit with the first screw hole, the first stepping motor (22) is positioned on one side of the first support (21), and a driving shaft of the first stepping motor (22) is coaxially arranged with the first spiral guide rod (23) and is connected with the first spiral guide rod;
the Y-axis driving assembly is installed on the first sliding table (24), the detection disc (4) is installed on the Y-axis driving assembly, the Y-axis driving assembly is used for driving the detection disc (4) to move in the direction perpendicular to the first sliding rail, and a plurality of sample detection positions are arranged on the detection disc (4);
mounting bracket (5) are installed on base (1) and are located detection dish (4) one side, and raman detector (7) are installed on mounting bracket (5) and are located detection dish (4) top and towards detection dish (4) setting.
2. The surface-enhanced Raman spectroscopy multi-sample detection system according to claim 1, wherein the Y-axis driving assembly comprises a second support (31), a second slide rail, a second stepping motor (32) and a second spiral guide rod (33), the second support (31) is mounted on the first slide table (24), the second slide rail is horizontally arranged on the first slide table (24) perpendicular to the first slide rail, the second slide rail is provided with a second slide table (34) which can be slidably mounted, the second slide table (34) is provided with a second screw hole which extends in parallel with the direction of the second slide rail, the second spiral guide rod (33) is rotatably mounted on the second support (31) and is in threaded fit with the second screw hole, the second stepping motor (32) is located on one side of the second support (31), and a driving shaft of the second stepping motor (32) is coaxially arranged with the second spiral guide rod (33) and is connected with the spiral guide rod, the detection disc (4) is arranged on the second sliding table (34).
3. The surface-enhanced Raman spectroscopy multi-sample detection system according to claim 2, further comprising an air pump (6), wherein a negative pressure cavity is arranged in the detection plate (4), an air suction hole is arranged at the top of the negative pressure cavity, an air outlet hole is arranged on the side wall of the negative pressure cavity, and the air pump (6) is connected with the air outlet hole through an air pipe.
4. The surface-enhanced Raman spectroscopy multi-sample detection system according to claim 3, wherein the detection tray (4) comprises a detection seat (41) and a sample stage (42), a receiving groove for receiving the sample stage (42) is formed at the top of the detection seat (41), and the suction hole is located in the receiving groove.
5. The surface-enhanced Raman spectroscopy multi-sample detection system according to claim 4, wherein the receiving groove extends from one end to a middle portion at a top of the detection seat (41).
6. The surface-enhanced raman spectroscopy multi-sample detection system according to claim 2, further comprising a control system connected to the first stepping motor (22) and/or the second stepping motor (32) for controlling the operation of the first stepping motor (22) and/or the second stepping motor (32).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911383821.7A CN111060493A (en) | 2019-12-28 | 2019-12-28 | Surface-enhanced Raman spectrum multi-sample detection system |
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| CN201911383821.7A CN111060493A (en) | 2019-12-28 | 2019-12-28 | Surface-enhanced Raman spectrum multi-sample detection system |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI796791B (en) * | 2021-09-16 | 2023-03-21 | 崇浩光電科技股份有限公司 | Two-dimensional scanning Raman spectroscopy detection system |
| TWI832092B (en) * | 2021-09-16 | 2024-02-11 | 國立臺北科技大學 | Two-dimensional scanning Raman spectroscopy detection system and detection method thereof |
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| TWI832092B (en) * | 2021-09-16 | 2024-02-11 | 國立臺北科技大學 | Two-dimensional scanning Raman spectroscopy detection system and detection method thereof |
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Application publication date: 20200424 |