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CN111032883A - 核酸测序方法 - Google Patents

核酸测序方法 Download PDF

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Publication number
CN111032883A
CN111032883A CN201780093472.8A CN201780093472A CN111032883A CN 111032883 A CN111032883 A CN 111032883A CN 201780093472 A CN201780093472 A CN 201780093472A CN 111032883 A CN111032883 A CN 111032883A
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China
Prior art keywords
sequencing
reaction
nucleic acid
solid support
solution
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CN201780093472.8A
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English (en)
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CN111032883B (zh
Inventor
杨晋
徐讯
王卉
谢槟
李卓坤
赵胜明
陈奥
徐崇钧
章文蔚
倪鸣
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MGI Tech Co Ltd
Complete Genomics Inc
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MGI Tech Co Ltd
Complete Genomics Inc
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Application filed by MGI Tech Co Ltd, Complete Genomics Inc filed Critical MGI Tech Co Ltd
Priority to CN202410439381.7A priority Critical patent/CN118086478A/zh
Priority to CN202410439618.1A priority patent/CN118086480A/zh
Priority to CN202410439616.2A priority patent/CN118109572A/zh
Priority to CN202410439435.XA priority patent/CN118086479A/zh
Publication of CN111032883A publication Critical patent/CN111032883A/zh
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Publication of CN111032883B publication Critical patent/CN111032883B/zh
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

本发明提供一种使用浸泡式反应方案对核酸进行测序的方法,所述浸泡式反应方案包括使其上固定有核酸分子的固体支持物依次浸泡在不同的反应容器中以实现核酸测序。

Description

PCT国内申请,说明书已公开。

Claims (22)

  1. PCT国内申请,权利要求书已公开。
CN201780093472.8A 2017-08-01 2017-08-01 核酸测序方法 Active CN111032883B (zh)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN202410439381.7A CN118086478A (zh) 2017-08-01 2017-08-01 核酸测序方法
CN202410439618.1A CN118086480A (zh) 2017-08-01 2017-08-01 核酸测序方法
CN202410439616.2A CN118109572A (zh) 2017-08-01 2017-08-01 核酸测序方法
CN202410439435.XA CN118086479A (zh) 2017-08-01 2017-08-01 核酸测序方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2017/095520 WO2019023951A1 (zh) 2017-08-01 2017-08-01 核酸测序方法

Related Child Applications (4)

Application Number Title Priority Date Filing Date
CN202410439435.XA Division CN118086479A (zh) 2017-08-01 2017-08-01 核酸测序方法
CN202410439381.7A Division CN118086478A (zh) 2017-08-01 2017-08-01 核酸测序方法
CN202410439616.2A Division CN118109572A (zh) 2017-08-01 2017-08-01 核酸测序方法
CN202410439618.1A Division CN118086480A (zh) 2017-08-01 2017-08-01 核酸测序方法

Publications (2)

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CN111032883A true CN111032883A (zh) 2020-04-17
CN111032883B CN111032883B (zh) 2024-05-03

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CN202410439618.1A Pending CN118086480A (zh) 2017-08-01 2017-08-01 核酸测序方法
CN202410439435.XA Pending CN118086479A (zh) 2017-08-01 2017-08-01 核酸测序方法
CN201780093472.8A Active CN111032883B (zh) 2017-08-01 2017-08-01 核酸测序方法
CN202410439616.2A Pending CN118109572A (zh) 2017-08-01 2017-08-01 核酸测序方法
CN202410439381.7A Pending CN118086478A (zh) 2017-08-01 2017-08-01 核酸测序方法

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CN202410439435.XA Pending CN118086479A (zh) 2017-08-01 2017-08-01 核酸测序方法

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CN202410439381.7A Pending CN118086478A (zh) 2017-08-01 2017-08-01 核酸测序方法

Country Status (11)

Country Link
US (3) US11692221B2 (zh)
EP (3) EP4209597B1 (zh)
CN (5) CN118086480A (zh)
DK (1) DK3663407T3 (zh)
ES (1) ES2941083T3 (zh)
FI (1) FI3663407T3 (zh)
HU (1) HUE061474T2 (zh)
PL (1) PL3663407T3 (zh)
PT (1) PT3663407T (zh)
RS (1) RS64086B1 (zh)
WO (1) WO2019023951A1 (zh)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022104138A1 (en) * 2020-11-14 2022-05-19 Life Technologies Corporation System and method for automated repeat sequencing
CN115433675B (zh) * 2022-08-03 2024-02-23 深圳赛陆医疗科技有限公司 核酸测序系统及测序控制方法

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CN1106459A (zh) * 1993-10-26 1995-08-09 株式会社日立制作所 核苷酸片段的分级分离方法
CN101189345A (zh) * 2005-02-01 2008-05-28 Ab先进基因分析公司 珠基测序的试剂、方法和文库
US20100111768A1 (en) * 2006-03-31 2010-05-06 Solexa, Inc. Systems and devices for sequence by synthesis analysis
CN103993100A (zh) * 2014-06-17 2014-08-20 东南大学 一种提高基因连接测序准确性的方法

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DK3363809T3 (da) 2002-08-23 2020-05-04 Illumina Cambridge Ltd Modificerede nukleotider til polynukleotidsekvensering
DE60327649D1 (de) 2002-08-23 2009-06-25 Illumina Cambridge Ltd Markierte nukleotide
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
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US20060246576A1 (en) * 2005-04-06 2006-11-02 Affymetrix, Inc. Fluidic system and method for processing biological microarrays in personal instrumentation
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Publication number Priority date Publication date Assignee Title
WO1991006678A1 (en) * 1989-10-26 1991-05-16 Sri International Dna sequencing
CN1106459A (zh) * 1993-10-26 1995-08-09 株式会社日立制作所 核苷酸片段的分级分离方法
CN101189345A (zh) * 2005-02-01 2008-05-28 Ab先进基因分析公司 珠基测序的试剂、方法和文库
US20100111768A1 (en) * 2006-03-31 2010-05-06 Solexa, Inc. Systems and devices for sequence by synthesis analysis
CN103993100A (zh) * 2014-06-17 2014-08-20 东南大学 一种提高基因连接测序准确性的方法

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Also Published As

Publication number Publication date
CN111032883B (zh) 2024-05-03
RS64086B1 (sr) 2023-04-28
CN118086480A (zh) 2024-05-28
EP4209597A1 (en) 2023-07-12
HUE061474T2 (hu) 2023-07-28
CN118109572A (zh) 2024-05-31
US20200318177A1 (en) 2020-10-08
FI3663407T3 (fi) 2023-04-18
PT3663407T (pt) 2023-03-27
DK3663407T3 (da) 2023-04-03
PL3663407T3 (pl) 2023-05-08
EP4212628A1 (en) 2023-07-19
WO2019023951A1 (zh) 2019-02-07
CN118086478A (zh) 2024-05-28
EP4212628C0 (en) 2025-01-29
US11692221B2 (en) 2023-07-04
EP3663407B1 (en) 2023-03-15
ES2941083T3 (es) 2023-05-16
US20230323445A1 (en) 2023-10-12
CN118086479A (zh) 2024-05-28
EP4209597C0 (en) 2025-02-26
US20230272467A1 (en) 2023-08-31
EP3663407A1 (en) 2020-06-10
EP3663407A4 (en) 2021-03-03
EP4209597B1 (en) 2025-02-26
EP4212628B1 (en) 2025-01-29

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Address after: 518083 the comprehensive building of Beishan industrial zone and 11 2 buildings in Yantian District, Shenzhen, Guangdong.

Patentee after: Shenzhen Huada Zhizao Technology Co.,Ltd.

Country or region after: China

Patentee after: COMPLETE GENOMICS Inc.

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Address before: 518083 the comprehensive building of Beishan industrial zone and 11 2 buildings in Yantian District, Shenzhen, Guangdong.

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