CN111012694A - Hyaluronic acid-containing spray for repairing and moisturizing soles and preparation method thereof - Google Patents
Hyaluronic acid-containing spray for repairing and moisturizing soles and preparation method thereof Download PDFInfo
- Publication number
- CN111012694A CN111012694A CN201911403388.9A CN201911403388A CN111012694A CN 111012694 A CN111012694 A CN 111012694A CN 201911403388 A CN201911403388 A CN 201911403388A CN 111012694 A CN111012694 A CN 111012694A
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- CN
- China
- Prior art keywords
- spray
- molecular weight
- vitamin
- moisturizing
- hyaluronic acid
- Prior art date
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- 229920002674 hyaluronan Polymers 0.000 title claims abstract description 67
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 title claims abstract description 65
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- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims abstract description 93
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Classifications
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Abstract
Discloses a sole repair moisturizing spray containing hyaluronic acid and a preparation method thereof. The sole repairing and moisturizing spray containing hyaluronic acid is composed of super-active sodium hyaluronate, low and medium molecular weight sodium hyaluronate, zinc hyaluronate, low molecular weight zinc hyaluronate, centella asiatica extract, vitamins, amino acids, preservatives and purified water. The preparation method of the spray comprises weighing the components, dissolving, filtering, and filling into spray bottle to obtain water soluble spray. The spray can be used for treating foot chapping commonly existing in life, and has effects of moistening foot, inhibiting bacteria, relieving inflammation and repairing foot. The super-active sodium hyaluronate, the sodium hyaluronate with medium and low molecular weight, the zinc hyaluronate with low molecular weight and the centella asiatica are combined according to a certain mass proportion to generate interaction, so that a synergistic effect is generated, and the bacteriostatic and anti-inflammatory effects of the spray for repairing and moisturizing the sole of a foot are remarkably improved.
Description
Technical Field
The invention belongs to the field of medicines and cosmetics, and particularly relates to a sole repairing and moisturizing spray composition containing hyaluronic acid and a preparation method thereof.
Background
In autumn and winter, many people have different degrees of dry and split heels, sides and soles. Generally, the heel peeling and chapping are more serious, even bleeding is caused, and the bleeding symptoms are mostly appeared in middle-aged and elderly people. The main causes of the foot chap are as follows: firstly, in autumn and winter, the weather and the air temperature are unstable, and the moisture in the air is very rare, so that the moisture of the human body can be greatly separated out, the moisture of the human body is lost, and the skin is easy to have water deficiency and roughness, such as 'dry foot' symptom. Secondly, when adults age to a certain extent, the functional activities of various parts in the body are reduced, and the symptoms of 'dry and cracked feet' are easy to appear. Third, the lack of certain nutrients, such as vitamins, in the human body also leads to the "dry foot" condition. Fourth, in the current global environment, air pollution is serious, various bacteria and fungi are bred, fungal infection is easy to occur, and the symptom of 'dry foot' is also caused.
Hyaluronic acid, also known as hyaluronic acid or uronic acid, is a substance naturally present in human tissues. It plays an important role as a matrix in the dermis layer of human skin, largely filling the spaces between the cells and collagen fibers of the human body. It is mostly present in the skin, vitreous eye, umbilical cord, cartilage, joints, etc. of human body, and plays the roles of moisturizing, nourishing, repairing, etc. in the skin, the content of hyaluronic acid in the skin of human body is gradually reduced along with the increase of the age of adults, and the reduction of hyaluronic acid makes the skin gradually lose elasticity, so that the skin is rough and dull. However, the lack of the endogenous hyaluronic acid in human bodies can be supplemented by the exogenous hyaluronic acid, so the hyaluronic acid has good effects of moisturizing, lubricating and the like, and is widely applied to the aspects of skin care, medical cosmetology, medicines and the like. The use of sodium hyaluronate to solve the problem of "dry foot" has natural advantages. Generally, hyaluronic acid, which is a product of the type known as its sodium salt, can be classified into high molecular weight, medium molecular weight, low molecular weight and low molecular weight hyaluronic acids according to their molecular weights, and the properties of hyaluronic acids with different molecular weights are different.
The zinc hyaluronate has the functions of moisturizing, oxidation resistance, repair and the like of sodium hyaluronate, also has good bacteriostatic and anti-inflammatory effects, and has good repair effect on foot problems caused by fungi and bacteria. In addition, the publication No. CN 106309251B discloses a centella asiatica extract with anti-inflammatory and allergy-relieving effects and application thereof, and proves that the centella asiatica extract has good anti-inflammatory, allergy-relieving and anti-oxidation effects, has an outstanding effect of removing free radicals, and is widely applied to medicines and skin care products.
At present, the sodium hyaluronate used in the market is mostly macromolecular sodium hyaluronate with the molecular weight of 1800kDa-2200kDa and medium molecular weight sodium hyaluronate with the molecular weight of 200kDa-400 kDa. The pure sodium hyaluronate with large molecular weight or low molecular weight can only form a layer of transparent hydrated film on the surface layer of the skin, can only achieve a small water locking function, and is not easy to be absorbed by the skin. As a foot care product containing hyaluronic acid, chinese patent No. CN105943445B discloses a foot care solution and a preparation method thereof, in which the extraction of aloe peel juice is cumbersome and the effective utilization rate is low, and the preparation method is unstable, and an emulsification solubilizer is required to stably disperse its oil-soluble components in the solution. Chinese patent application publication No. CN107648119A discloses an essence emulsion containing camellia oil and centella asiatica extract and a preparation method thereof, the essence emulsion has only a whitening effect and a single effect, and in the preparation method, propylene glycol, essence and other substances harmful to human bodies are used in a high proportion.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the plantar repair moisturizing spray containing hyaluronic acid, which has good foot moisturizing, antibacterial, anti-inflammatory and repairing effects, can be used for daily foot care and can be used for improving the problem of foot chapping.
The invention also provides a preparation method of the sole repairing and moisturizing spray containing hyaluronic acid, wherein the water-soluble spray is prepared by compounding and dissolving sodium hyaluronate, sodium hyaluronate with medium and low molecular weights, zinc hyaluronate with low molecular weights, centella asiatica extract, preservative, vitamins, amino acids and purified water according to a certain proportion, uniformly mixing, filtering, and filling into spray bottles.
The invention adopts the following specific technical scheme.
1. A sole repair moisturizing spray containing hyaluronic acid is characterized by comprising the following components in percentage by mass: 0.20 to 0.60 percent of super-active sodium hyaluronate, 0.04 to 0.12 percent of medium-low molecular weight sodium hyaluronate, 0.03 to 0.09 percent of zinc hyaluronate, 0.10 to 0.30 percent of low molecular weight zinc hyaluronate, 1.00 to 3.00 percent of vitamin, 0.50 to 2.00 percent of amino acid, 0.50 to 1.50 percent of centella asiatica extract, 0.50 to 1.50 percent of preservative, and the balance of purified water.
2. The plantar repair moisturizing spray containing hyaluronic acid according to item 1, which is characterized in that the average molecular weight of the super-active sodium hyaluronate is less than 5kDa, the average molecular weight of the medium and low molecular weight sodium hyaluronate is 20 kDa-50 kDa, the average molecular weight of the zinc hyaluronate is 10 kDa-30 kDa, and the average molecular weight of the low molecular weight zinc hyaluronate is less than 10 kDa.
3. The spray containing hyaluronic acid for repairing and moisturizing sole according to item 1 or 2, wherein the vitamin is 2.00% by mass.
4. The spray containing hyaluronic acid for repairing and moisturizing sole according to claim 1, wherein the vitamin is at least one of vitamin C, vitamin B2 and vitamin E.
5. The sole repair moisturizing spray containing hyaluronic acid according to item 4, wherein the vitamin is a vitamin complex comprising vitamin C, vitamin B and/or vitamin B2, and vitamin E.
6. The sole repair and moisture preservation spray containing hyaluronic acid according to item 4, wherein the percentage content of vitamin C in the spray is 0.50% -1.00%, the percentage content of vitamin B and/or vitamin B2 in the spray is 0.1% -1%, and the percentage content of vitamin E in the spray is 0.50% -1.00%.
7. The spray containing hyaluronic acid for repairing and moisturizing sole according to item 5, wherein the mass ratio of vitamin C, vitamin B2 and vitamin E in the vitamin complex is 10:3: 4.
8. The sole repair moisturizing spray containing hyaluronic acid according to item 1, wherein the amino acid is 1.50% by mass.
9. The hyaluronic acid-containing plantar repair moisturizing spray according to claim 1, wherein the amino acid component is at least one selected from glycine, proline and histidine.
10. The hyaluronic acid-containing plantar repair moisturizing spray according to claim 9, characterized in that the amino acid component is an amino acid complex consisting of glycine, proline and histidine.
11. The sole repair moisturizing spray containing hyaluronic acid according to item 1, characterized in that the mass ratio of the super-active sodium hyaluronate, the medium-low molecular weight sodium hyaluronate, the zinc hyaluronate, the low molecular weight zinc hyaluronate and the centella asiatica extract is 10-30: 2-6: 1.5-4.5: 5-15: 25-75, preferably 20: 4: 3: 10: 50.
12. The sole repair moisturizing spray containing hyaluronic acid according to item 1, wherein the preservative is 0.80% by mass.
13. The sole repair moisturizing spray containing hyaluronic acid according to claim 1, wherein the preservative is a combination of phenoxyethanol and pentanediol.
14. The sole repair moisturizing spray containing hyaluronic acid according to claim 13, wherein the mass ratio of pentanediol to phenoxyethanol in the preservative is 1: 3.
15. The hyaluronic acid-containing plantar repair moisturizing spray according to any one of claims 1-14, which is characterized in that the kinematic viscosity of the spray is 10-80 mpa.s.
16. The preparation method of the hyaluronic acid-containing plantar repair moisturizing spray according to any one of claims 1-15, characterized by comprising the following steps:
(1) weighing: accurately weighing the components according to the component proportion of the hyaluronic acid-containing foot sole repairing and moisturizing spray.
(2) Dissolving: adding the preservative into a proper amount of water, and fully stirring and dissolving; then adding the super-active sodium hyaluronate, the sodium hyaluronate with medium and low molecular weight, the zinc hyaluronate with low molecular weight, the zinc hyaluronate, the centella asiatica extract, the vitamins and the amino acid into the mixture, and stirring the mixture until the mixture is completely dissolved.
(3) And (3) filtering: filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
(4) Filling: packaging into spray bottle to obtain aqueous spray. The spray bottle is made of aluminum, and is sealed in vacuum.
17. The method for preparing a hyaluronic acid-containing spray for repairing and moisturizing sole according to item 16, wherein the water temperature for dissolution in step (2) is 20 ℃ to 38 ℃.
According to the invention, through compounding sodium hyaluronate with different molecular weights, particularly compounding the super-active sodium hyaluronate with medium and low molecular weights, the sole repairing and moisturizing spray containing hyaluronic acid has the functions of oxidation resistance, aging resistance, skin repairing and the like while achieving a better moisturizing effect. In addition, the zinc hyaluronate also has better moisturizing, anti-inflammatory and bacteriostatic effects, and the efficacy of the sole repair moisturizing spray containing hyaluronic acid is further improved. The super-active sodium hyaluronate, the sodium hyaluronate with medium and low molecular weight, the zinc hyaluronate with low molecular weight and the centella asiatica extract are combined according to a certain mass proportion to generate interaction, so that a synergistic effect is generated, and the foot sole repairing and moisturizing spray has better bacteriostatic and anti-inflammatory effects.
The sole repair moisturizing spray containing hyaluronic acid has the effects of moisturizing feet, resisting inflammation and inhibiting bacteria, promoting wound healing, repairing and the like, and can be suitable for symptoms of heel, foot side, sole chap and the like with different degrees.
Detailed description of the invention
The present invention will be described in detail below.
The invention provides a sole repair moisturizing spray containing hyaluronic acid, which is characterized by comprising the following components in percentage by mass: 0.20 to 0.60 percent of super-active sodium hyaluronate, 0.04 to 0.12 percent of medium-low molecular weight sodium hyaluronate, 0.03 to 0.09 percent of zinc hyaluronate, 0.10 to 0.30 percent of low molecular weight zinc hyaluronate, 1.00 to 3.00 percent of vitamin, 0.50 to 2.00 percent of amino acid, 0.50 to 1.50 percent of centella asiatica extract, 0.50 to 1.50 percent of preservative, and the balance of purified water.
Two kinds of sodium hyaluronate with different molecular weights are compounded and used in the invention, including super-active sodium hyaluronate with small molecular weight and sodium hyaluronate with medium and low molecular weight. The molecular weight of the sodium hyaluronate has great influence on the biological activity of the sodium hyaluronate, and the sodium hyaluronate with different molecular weight ranges has distinct physiological functions. The general hyaluronic acid cannot be absorbed by skin due to high molecular weight, for example, macromolecular sodium hyaluronate (average molecular weight is more than 1000kDa) can form a film, can prevent water from evaporating, can play a moisturizing role within a certain time, but loses the effect after about 8 hours, so the large molecular weight sodium hyaluronate cannot achieve a long-acting moisturizing effect. The invention selects sodium hyaluronate with medium and low molecular weight, and particularly preferably sodium hyaluronate with average molecular weight of 20 kDa-50 kDa. Preferred specific average molecular weights of the low or medium molecular weight sodium hyaluronate of the present invention are 20kDa, 30kD or 50 kDa. The water-based moisture-preserving cream has better permeability and long-acting moisture-preserving effect, and the moisture-preserving time generally exceeds 8 hours.
The sole repair moisture-keeping spray containing hyaluronic acid preferably contains sodium hyaluronate with medium and low molecular weight in the content of 0.04-0.12 wt%, such as 0.04 wt%.
The sole repairing and moisturizing spray containing hyaluronic acid simultaneously uses sodium hyaluronate with small molecular weight, preferably average molecular weight less than 5kDa as the super-active sodium hyaluronate. The preferred specific average molecular weight of the small molecular weight sodium hyaluronate of the present invention is 2kDa, 3kD or 4 kDa. The molecular size of the sodium hyaluronate reaches the nanometer level, the sodium hyaluronate is easy to permeate the skin and can permeate to the depth of the dermis, namely, the sodium hyaluronate has good transdermal absorbability, has the function similar to a signal molecule in the skin, can cause a series of biological reactions and has better capacities of resisting oxidation and scavenging free radicals. By using the super-active sodium hyaluronate, not only can more effective moisturizing effect be provided, but also certain skin repairing effect and anti-aging effect can be further provided for the foot sole repairing moisturizing spray.
The sole repair moisture-keeping spray containing hyaluronic acid preferably contains 0.20-0.60% of super-active sodium hyaluronate, such as 0.20%, 0.40% and the like in percentage by mass.
The sole repair moisturizing spray containing hyaluronic acid also comprises zinc hyaluronate, and the average molecular weight of the zinc hyaluronate is preferably 10 kDa-30 kDa. Zinc hyaluronate according to the invention preferably has a specific average molecular weight of 10kDa, 20kD or 30 kDa. The zinc hyaluronate has good biocompatibility and viscosity, and can form a protective film on the surface of a skin wound, repair wound tissues and promote wound healing. The mass percentage of the zinc hyaluronate in the spray for repairing and moisturizing sole is 0.03-0.09%, such as 0.04%, 0.05%, 0.06% and the like. In addition, the spray for repairing and moisturizing the sole of the foot also comprises low-molecular-weight zinc hyaluronate, so that the solubility and stability of zinc hyaluronate in water are improved, and the absorbability of zinc hyaluronate in the skin is improved. The average molecular weight of the low molecular weight zinc hyaluronate is preferably 10kDa or less, and the specific average molecular weight of the low molecular weight zinc hyaluronate of the present invention is preferably 6kDa, 8kD or 9 kDa. The mass percentage content of the foot bottom repairing and moisturizing spray is 0.10-0.30%, such as 0.2%.
The hyaluronic acid-containing spray for repairing and moisturizing sole contains vitamins, and the vitamins in the invention are not particularly limited, and can be single vitamins or compound vitamins consisting of multiple vitamins. The vitamin is preferably at least one of vitamin C, vitamin B2, and vitamin E. Particularly preferably, the vitamin is a compound vitamin containing vitamin C, vitamin B and/or vitamin B2, and vitamin E.
The mass percentage content of the vitamin in the sole repairing and moisturizing spray is 1.00-3.00%, and preferably 2.00%. In addition, the content of the vitamin C in the spray is preferably 0.50-1.00 percent by mass, the content of the vitamin B and/or vitamin B2 in the spray is preferably 0.10-1.00 percent by mass, and the content of the vitamin E in the spray is preferably 0.50-1.00 percent by mass. Preferably, the mass ratio of the vitamin C, the vitamin B2 and the vitamin E in the compound vitamin component is 10:3: 4.
The amino acid component contained in the hyaluronic acid-containing spray for repairing and moisturizing the sole of a foot of the present invention is not particularly limited, and may be a single amino acid or an amino acid complex containing a plurality of amino acids. The mass percentage content is 0.50-2.00%, preferably 1.50%. The amino acid component is preferably at least one of glycine, proline, and histidine. More preferably, the amino acid is an amino acid complex consisting of glycine, proline and histidine.
The hyaluronic acid-containing plantar repairing moisturizing spray contains the centella asiatica extract, and the component has important effects of promoting collagen synthesis in vivo, neovascularization and the like and is beneficial to wound healing. The mass percentage of the asiatic pennywort herb extract in the plantar repairing and moisturizing spray is 0.50-1.50%, and the applicant finds that the asiatic pennywort herb extract can well solve the problem of 'dry foot'.
The mass ratio of the super-active sodium hyaluronate, the sodium hyaluronate with medium and low molecular weight, the zinc hyaluronate with low molecular weight and the centella asiatica extract in the spray for repairing and moisturizing the sole containing the hyaluronic acid is 10-30: 2-6: 1.5-4.5: 5-15: 25-75, and the preferred ratio is 20: 4: 3: 10: 50.
The preservative contained in the hyaluronic acid-containing spray for repairing and moisturizing the sole of a foot of the present invention is not particularly limited as long as it can inhibit microorganisms such as bacteria, molds, yeasts and the like in the preparation, and may be a preservative antibacterial agent commonly used in medicines and cosmetics. The mass percentage of the preservative in the sole repairing and moisturizing spray is 0.50-1.50%, and the preferred mass percentage is 0.80%. The preferred preservative is a combination of phenoxyethanol and pentanediol, and more preferably the preservative has a mass ratio of pentanediol to phenoxyethanol of 1: 3.
In addition to the above components, the spray for repairing and moisturizing sole containing hyaluronic acid of the present invention also contains a large amount of purified water as a solvent. The sole repair moisturizing spray containing hyaluronic acid is liquid with good fluidity, and the kinematic viscosity of the liquid is 10-80 mPa.s.
The invention also provides a preparation method of the hyaluronic acid-containing plantar repairing moisturizing spray, and the specific process steps comprise weighing, dissolving, filtering, filling and the like.
The method comprises the following specific steps:
(1) weighing: accurately weighing the components according to the component proportion of the hyaluronic acid-containing foot sole repairing and moisturizing spray.
(2) Dissolving: adding antiseptic into appropriate amount of water, stirring for dissolving, adding super-active sodium hyaluronate, sodium hyaluronate with medium and low molecular weight, zinc hyaluronate with low molecular weight, zinc hyaluronate, herba Centellae extract, compound vitamins, and amino acid compound, and stirring for dissolving completely. The water temperature for the dissolution is 20 to 38 ℃.
(3) And (3) filtering: filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
(4) Filling: packaging into spray bottle to obtain aqueous spray. The spray bottle is an aluminum spray bottle, is sealed in vacuum, is light-proof and has good spraying effect.
The sole repair moisturizing spray containing hyaluronic acid provided by the invention is prepared by compounding multiple specific hyaluronate salts, wherein zinc hyaluronate, low molecular weight zinc hyaluronate and super-active sodium hyaluronate have an obvious synergistic effect in anti-inflammatory repair, so that the sole repair moisturizing spray has good moisturizing, antibacterial, anti-inflammatory and wound repairing effects on foot skin, and can be used for daily foot care and repairing problems of foot chapping.
The sole repair moisturizing spray containing hyaluronic acid has the following beneficial effects:
(1) contains sodium hyaluronate with different molecular weights, has the effects of relieving and moisturizing, inhibiting bacteria, diminishing inflammation, repairing wound and healing wound, and comprehensively takes care of feet.
(2) Contains high and low molecular weight zinc hyaluronate, has synergistic antiinflammatory and repairing effects, and can be used for repairing and caring foot dryness and chap.
(3) The super-active sodium hyaluronate, the sodium hyaluronate with medium and low molecular weight, the zinc hyaluronate with low molecular weight and the centella asiatica extract are combined according to a certain mass proportion and can interact with each other to generate a synergistic effect, so that the foot sole repairing and moisturizing spray has better bacteriostatic and anti-inflammatory effects.
(4) Contains nutritional components (such as amino acids and vitamins) which are lacked by human body, and can fully supplement skin nutrition.
(5) The centella asiatica has the effects of resisting inflammation, relieving allergy, resisting oxidation, solving the problems of dry and tight feet and relieving pain.
(6) The foot spray has the advantages of convenient carrying, directional administration, quick absorption, no bacterial contamination and the like.
Examples
The present invention is further illustrated by the following examples. The following examples of the invention are intended only to illustrate specific embodiments for carrying out the invention and these embodiments should not be construed as limiting the invention. Other changes, modifications, substitutions, combinations, and simplifications which may be made without departing from the spirit and principles of the invention are intended to be equivalents thereof and to fall within the scope of the invention.
The experimental methods used in the following examples are conventional methods unless otherwise specified, and materials, reagents and the like used therein are commercially available without further specification.
Example 1
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.60% of super-active sodium hyaluronate (average molecular weight of 4kDa), 0.12% of medium-low molecular weight sodium hyaluronate (average molecular weight of 20kDa), 0.09% of zinc hyaluronate (average molecular weight of 20kDa), 0.30% of low-molecular weight zinc hyaluronate (8kDa), 2.00% of vitamin complex (vitamin C1.00%, vitamin B0.30%, vitamin B20.30%, vitamin E0.40%), 1.50% of amino acid complex (glycine 0.50%, proline 0.50% and histidine 0.50%), 1.50% of centella asiatica extract, 0.80% of preservative (pentanediol 0.20% and phenoxyethanol 0.60%), and purified water to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
(4) Filling: packaging into spray bottle to obtain water soluble spray.
Example 2
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.40% of super-active sodium hyaluronate (average molecular weight of 4kDa), 0.08% of medium-low molecular weight sodium hyaluronate (average molecular weight of 30kDa), 0.06% of zinc hyaluronate (average molecular weight of 20kDa), 0.20% of low molecular weight zinc hyaluronate (average molecular weight of 6kDa), 1.00% of vitamin complex (vitamin C0.50%, vitamin B0.50%), 1.50% of amino acid complex (glycine 1.00%, proline 0.50%), 1.00% of centella asiatica extract, 0.80% of preservative (pentanediol 0.20% and phenoxyethanol 0.60%), and purified water to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
(4) Filling: packaging into spray bottle to obtain water soluble spray.
Example 3
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.30% of super-active sodium hyaluronate (average molecular weight of 3kDa), 0.06% of medium-low molecular weight sodium hyaluronate (average molecular weight of 20kDa), 0.50% of zinc hyaluronate (average molecular weight of 30kDa), 0.15% of low-molecular weight zinc hyaluronate (average molecular weight of 8kDa), 1.00% of vitamin complex (vitamin B20.40%, vitamin E0.60%), 1.50% of amino acid complex (glycine 0.50%, proline 0.50% and histidine 0.50%), 0.75% of centella asiatica extract, 0.80% of preservative (pentanediol 0.20% and phenoxyethanol 0.60%), and water supplement to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
(4) Filling: packaging into spray bottle to obtain water soluble spray.
Example 4
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.20% of super-active sodium hyaluronate (average molecular weight of 3kDa), 0.04% of medium-low molecular weight sodium hyaluronate (average molecular weight of 50kDa), 0.03% of zinc hyaluronate (average molecular weight of 30kDa), 0.10% of low-molecular weight zinc hyaluronate (average molecular weight of 9kDa), 1.00% of vitamin complex (vitamin B20.40%, vitamin E0.60%), 1.50% of amino acid complex (glycine 0.75%, proline 0.75%), 0.50% of centella asiatica extract, 0.80% of preservative (pentanediol 0.30% and phenoxyethanol 0.50%), and purified water is supplemented to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
(4) Filling: packaging into spray bottle to obtain water soluble spray.
Example 5
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.40% of super-active sodium hyaluronate (average molecular weight of 3kDa), 0.08% of medium-low molecular weight sodium hyaluronate (average molecular weight of 30kDa), 0.06% of zinc hyaluronate (average molecular weight of 20kDa), 0.10% of low molecular weight zinc hyaluronate (average molecular weight of 9kDa), 1.00% of vitamin complex (vitamin C0.50%, vitamin B0.50%), 1.50% of histidine, 1.00% of centella asiatica extract, 0.80% of preservative (0.50% of pentanediol and 0.30% of phenoxyethanol), and water is supplemented to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
(4) Filling: packaging into spray bottle to obtain water soluble spray.
Example 6
The foot repairing and moisturizing spray comprises the following components in percentage by mass based on the total amount of the foot repairing and moisturizing spray: 0.20% of super-active sodium hyaluronate (average molecular weight of 3kDa), 0.04% of medium-low molecular weight sodium hyaluronate (average molecular weight of 50kDa), 0.03% of zinc hyaluronate (average molecular weight of 10kDa), 0.10% of low-molecular weight zinc hyaluronate (average molecular weight of 8kDa), 2.00% of vitamin complex (vitamin C1.50%, vitamin B0.50%), 1.50% of proline and 0.50% of centella asiatica extract; 0.80% of preservative (0.50% of pentanediol and 0.30% of phenoxyethanol) and purified water added to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
(4) Filling: packaging into spray bottle to obtain water soluble spray.
Comparative example 1
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.08% of zinc hyaluronate (average molecular weight of 20kDa), 0.30% of low-molecular-weight zinc hyaluronate (average molecular weight of 6kDa), 2.00% of vitamin complex (vitamin C1.00%, vitamin B1.00%), 1.50% of histidine, 1.50% of centella asiatica extract, 0.80% of preservative (0.40% of pentanediol and 0.40% of phenoxyethanol), and purified water is added to reach 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
Comparative example 2
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.10% of super-active sodium hyaluronate (average molecular weight of 3kDa), 0.04% of medium-low molecular weight sodium hyaluronate (average molecular weight of 30kDa), 0.02% of zinc hyaluronate (average molecular weight of 30kDa), 0.08% of low-molecular weight zinc hyaluronate (average molecular weight of 6kDa), 2.00% of vitamin complex (vitamin B21.00%, vitamin E1.00%), 1.50% of proline, 1.50% of centella asiatica extract, 0.80% of preservative (0.30% of pentanediol and 0.50% of phenoxyethanol), and water is supplemented to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
Comparative example 3
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.60% of super-active sodium hyaluronate (average molecular weight of 4kDa), 0.02% of medium-low molecular weight sodium hyaluronate (average molecular weight of 30kDa), 0.05% of zinc hyaluronate (average molecular weight of 30kDa), 0.40% of low-molecular weight zinc hyaluronate (average molecular weight of 8kDa), 2.00% of vitamin complex (vitamin B21.00%, vitamin E1.00%), 1.50% of glycine, 0.50% of centella asiatica extract, 0.80% of preservative (0.50% of pentanediol and 0.30% of phenoxyethanol), and water is supplemented to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
Comparative example 4
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.05% of super-active sodium hyaluronate (average molecular weight of 4kDa), 0.02% of medium-low molecular weight sodium hyaluronate (average molecular weight of 50kDa), 0.01% of zinc hyaluronate (average molecular weight of 30kDa), 0.03% of low molecular weight zinc hyaluronate (average molecular weight of 8kDa), 2.00% of vitamin complex (vitamin C1.50%, vitamin B0.50%), 1.50% of amino acid complex (glycine 0.50%, proline 0.50% and histidine 0.50%), 1.50% of centella asiatica extract, 0.80% of preservative (pentanediol 0.50% and phenoxyethanol 0.30%), and water supplement to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering: filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
Comparative example 5
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.40% of super-active sodium hyaluronate (average molecular weight of 4kDa), 0.10% of medium-low molecular weight sodium hyaluronate (average molecular weight of 20kDa), 0.01% of zinc hyaluronate (average molecular weight of 30kDa), 0.20% of low molecular weight zinc hyaluronate (average molecular weight of 8kDa), 2.00% of vitamin complex (vitamin B21.50%, vitamin E0.50%), 1.50% of amino acid complex (glycine 0.75%, proline 0.75%), 1.50% of centella asiatica extract, 0.80% of preservative (pentanediol 0.40% and phenoxyethanol 0.40%), and purified water is supplemented to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
Comparative example 6
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.60% of super-active sodium hyaluronate (average molecular weight of 3kDa), 0.09% of medium-low molecular weight sodium hyaluronate (average molecular weight of 30kDa), 0.09% of zinc hyaluronate (average molecular weight of 20kDa), 0.40% of low molecular weight zinc hyaluronate (average molecular weight of 6kDa), 2.00% of multivitamins (vitamin B20.50% and vitamin E0.50%), 1.50% of amino acid complex (proline 0.75% and histidine 0.75%), 1.50% of centella asiatica extract, 0.80% of preservative (pentanediol 0.40% and phenoxyethanol 0.40%), and purified water is supplemented to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
Comparative example 7
The mass percentage of each component is as follows based on the total amount of the sole repairing moisturizing spray: 0.80% of super-active sodium hyaluronate (average molecular weight of 2kDa), 0.15% of medium-low molecular weight sodium hyaluronate (average molecular weight of 50kDa), 0.03% of zinc hyaluronate (average molecular weight of 20kDa), 0.40% of low-molecular weight zinc hyaluronate (average molecular weight of 6kDa), 2.00% of vitamin complex (vitamin C0.50%, vitamin B20.50%, vitamin E0.50%), 1.50% of amino acid complex (glycine 1.00%, histidine 0.50%), 1.50% of centella asiatica extract, 0.80% of preservative (0.40% of pentanediol and 0.40% of phenoxyethanol), and water purification to 100%. The spray is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. Adding other components required in the spray into the solution 1, and stirring to completely dissolve.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
Comparative example 8
An existing medical sodium hyaluronate repair patch essence. The essence formula comprises the following components in percentage by mass: 0.80% of macromolecular sodium hyaluronate (average molecular weight of 1800kDa), 0.20% of low molecular weight sodium hyaluronate (average molecular weight of 350kDa), 0.50% of micromolecular sodium hyaluronate (average molecular weight of 7kDa), 0.10% of acetylated hyaluronic acid (average molecular weight of 30kDa), 0.20% of macromolecular sodium polyglutamate (average molecular weight of 1000kDa), 0.20% of small molecular sodium polyglutamate (average molecular weight of 120kDa), 0.30% of micromolecular sodium polyglutamate (average molecular weight of 5kDa), 0.80% of preservatives (phenoxyethanol 0.40% and pentanediol 0.40%), and water is supplemented to 100%. The essence is prepared by the following steps:
(1) weighing: accurately weighing and preparing the materials according to the component proportion.
(2) Dissolving: dissolving phenoxyethanol and pentanediol with appropriate amount of purified water, and dispersing to obtain solution 1. The other components were added to the solution 1 and stirred until completely dissolved.
(3) And (3) filtering: and (3) filtering the solution obtained in the step (2) through a 0.22 mu m filter membrane or a filter element for sterilization, and simultaneously filtering insoluble impurities.
Test example 1:
1. efficacy test of moisturizing Effect
Selecting 56 volunteers (female 30-50 years old) with foot chapping symptoms, having no serious system diseases and no immune or allergic diseases, not using foot-related nursing liquid during the test period, and simultaneously completing the test according to the test. Samples of the above formulation were used for short-term moisturizing (1 day) efficacy experiments on volunteer feet.
The 56 volunteers were evenly divided into groups, the initial moisture content of the skin of the foot was measured for the volunteers of this test, and the volunteers with the initial moisture content of the foot close to that (deviation value within the range of 2-10%) were selected for the test. Each group of 4 persons was subjected to short-term moisturizing effect efficacy experiments using different samples. The assay environment was as follows: the environment is indoor, strong sunlight or direct light is avoided, the environment temperature is 20 ℃ (the temperature fluctuates by 2 ℃) and the environment humidity is 30-60%.
Foot moisturizing tests were performed: the method comprises the following steps of prohibiting a subject from using a product with a moisturizing effect 2-3 days before a test, uniformly cleaning foot skin before the subject uses a sample, measuring an initial value of foot skin moisture of a tested area by using a German Corneometer skin moisture measuring instrument, fixedly measuring 5 points on the foot of the subject according to a certain sequence, measuring skin moisture of each point, and obtaining an average value. Namely the initial value of the moisture content of the foot skin. The sample is divided into opaque and graduated sample bottles, and the tested sample is sprayed on a fixed 5-point area for measuring the initial moisture of feet by a test subject, wherein the spraying amount of the sample is about 0.3-0.5 ml each time. After the feet are dried for about 20min, the shoes and socks can be worn, the moisture of the skin of the tested feet is measured by a German Corneometer skin moisture meter after the samples are used for 1 hour, 2 hours, 3 hours and 6 hours respectively, and the conclusion is made after the data is collated. The determination principle is as follows: the water and other substances have a considerable variation in dielectric constant (< 7), and depending on the water content, a suitably shaped measuring capacitor changes with the capacitance of the skin, which is within the measurement range, so that the moisture content of the skin can be measured.
The average foot moisture value for each hour (measurement value 1+ measurement value 2+ measurement value 3+ measurement value 4)/4 measurement value 1 (test point 1+ test point 2+ test point 3+ test point 4+ test point 5)/5.
TABLE 2 foot moisture measurements
Remarking: comparative examples 6 to 8 were applied directly to the feet due to their high viscosity, and the moisture content of the feet was measured 1 hour, 2 hours, 3 hours, and 6 hours after application, respectively.
From the above experimental data, it can be seen that the spray formulations (examples 1-6) containing higher contents of the ultra-active sodium hyaluronate and the medium and low molecular weight sodium hyaluronate have better short-term moisturizing effect on patients with dry foot cracks, compared to comparative examples 1 and 2. The comparison of the moisturizing test data of the examples and the comparative examples fully proves that the sodium hyaluronate spray provided by the invention has a good moisturizing effect on dry cracks of feet.
Test example 2:
efficacy test for promoting wound repair and healing effects
The following typical cases:
1. for a woman in age 28, the heel had a slight chap on the outside of the foot, no pain was felt, and some of the chap was rolled up around the laceration. After the skin of the heel is cleaned every day, the foot spray is sprayed to the fissure part three times in the morning, at noon and at night, and socks are worn after the foot spray is used, so that the dry fissure part is not touched. After one week of continuous use, the subject's heel and plantar cleft healed significantly, and after two weeks of use, the cleft disappeared and the skin of the foot became soft and smooth.
2. 3-5 chapped wounds with different degrees appear at the heel part of a woman in age of 35, and the woman has slight pain, deeper wounds and slight blood streak. After the skin of the heel is cleaned every day, the foot spray is sprayed to the fissure part three times in the morning, at noon and at night, and socks are worn after the foot spray is used, so that the dry fissure part is not touched. After two weeks of continuous use, the laceration on the foot becomes shallow, no blood is generated, the pain is eliminated, and after one month of use, the laceration is eliminated and the skin on the foot becomes soft, fine and smooth.
3. For a male, 25 years old, there are gaps at the bottom of the heel and on the inner and outer sides of the heel, and the dry and cracked feet last for about 2 years, with shallow gaps and no pain. After the skin of the heel is cleaned every day, the foot spray is sprayed to the fissure part three times in the morning, at noon and at night, and socks are worn after the foot spray is used, so that the dry fissure part is not touched. After two weeks of continuous use, the cleft on the sole of the foot heals better, and slight clefts are still formed on the inner side and the outer side of the foot, so that after one month of use, the cleft disappears and the skin of the foot returns to normal.
4. In 48 years old, a male has dry and cracked feet all the year round, deep wound, red and swollen bleeding around the wound, and can not walk in the ground in severe cases. The symptoms are relieved in spring and summer, and are severe in autumn and winter. After the skin of the heel is cleaned every day, the foot spray is sprayed to the fissure part three times in the morning, at noon and at night, and socks are worn after the foot spray is used, so that the dry fissure part is not touched. After the foot is continuously used for one month, the cleft on the foot bottom is well healed, the symptoms of redness and swelling and bleeding of the cleft disappear, and after the foot is continuously used for two months, the cleft is completely healed and the skin of the foot returns to be normal.
Test example 3
Anti-inflammatory efficacy test
The Balbc3T3 mouse embryonic fibroblasts are used as model cells, the model cells are stimulated by LPS to secrete inflammatory factors, different samples are used for processing, the expression of the inflammatory factors in culture supernatant is quantitatively detected, the inhibition effect of the different samples on the release of the inflammatory factors is examined, the examined factors comprise IL-6 and TGF- β 1, and the detection method is an ELISA method.
Test samples: example 1, example 2, example 5, comparative example 1, comparative example 3, comparative example 5, comparative example 8.
LPS (sigma, 025M4040V) mother liquor with the concentration of 50 ten thousand units/mL is prepared by serum-free 1640(Gibco) culture solution, filtered and sterilized by a 0.22 mu M filter membrane, stored in a refrigerator at the temperature of-20 ℃, and diluted into action solution with 1 ten thousand units/mL before use. The test samples were prepared at a concentration of 0.4% with LPS working solution and sterilized by filtration through a 0.22 μm filter. 3T3 cells were seeded at 1X 105/ml in 24-well plates using 1640 medium plus 10% fetal bovine serum (Gibco) at 37 ℃ with 5% CO2Culturing for 24h under the condition, adding a test sample, simultaneously taking LPS as a model control, continuously culturing for 24h, and detecting inflammatory factors by using a Mouse IL-6 ELISA Kit (Beijing four-positive-cypress Biotech limited, 20160519) Mouse TGF- β 1 ELISA Kit (Beijing four-positive-cypress Biotech limited, 20160519) Kit.
TABLE 3 inhibition ratio (%), of different samples, on inflammatory factors
| Example 1 | Example 2 | Example 5 | Comparative example 1 | Comparative example 3 | Comparative example 5 | Comparative example 8 | |
| IL-6 | 71.86 | 69.89 | 62.76 | 16.79 | 20.58 | 24.68 | 50.16 |
| TGF-β1 | 30.78 | 23.66 | 18.18 | 7.32 | 9.13 | 10.26 | 16.78 |
The result shows that for IL-6 and TGF- β 1, the higher the content of the super-active sodium hyaluronate, the better the inhibition effect, and the obvious advantage of the super-active sodium hyaluronate in anti-inflammatory repair is achieved, while the combination of the low and medium molecular weight sodium hyaluronate, zinc hyaluronate, low molecular weight zinc hyaluronate and centella asiatica extract according to a certain mass ratio has an obvious synergistic effect with the super-active sodium hyaluronate in anti-inflammatory repair.
Claims (10)
1. A sole repair moisturizing spray containing hyaluronic acid is characterized by comprising the following components in percentage by mass: 0.20 to 0.60 percent of super-active sodium hyaluronate, 0.04 to 0.12 percent of medium-low molecular weight sodium hyaluronate, 0.03 to 0.09 percent of zinc hyaluronate, 0.10 to 0.30 percent of low molecular weight zinc hyaluronate, 1.00 to 3.00 percent of vitamin, 0.50 to 2.00 percent of amino acid, 0.50 to 1.50 percent of centella asiatica extract, 0.50 to 1.50 percent of preservative, and the balance of purified water.
2. The sole repair moisture-retention spray containing hyaluronic acid according to claim 1, wherein the average molecular weight of the super-active sodium hyaluronate is less than 5kDa, the average molecular weight of the medium and low molecular weight sodium hyaluronate is 20kDa to 50kDa, the average molecular weight of the zinc hyaluronate is 10kDa to 30kDa, and the average molecular weight of the low molecular weight zinc hyaluronate is less than 10 kDa.
3. The spray containing hyaluronic acid for repairing and moisturizing sole according to claim 1 or 2, characterized in that the content of vitamin is 2.00% by mass.
4. The sole repair moisture-retaining spray containing hyaluronic acid according to claim 1, wherein the vitamin is at least one of vitamin C, vitamin B2 and vitamin E.
5. The plantar repair moisturizing spray containing hyaluronic acid according to claim 4, wherein the vitamin is a vitamin complex comprising vitamin C, vitamin B and/or vitamin B2, and vitamin E.
6. The sole repair and moisture retention spray containing hyaluronic acid according to claim 4, wherein the percentage content of vitamin C in the spray is 0.50-1.00%, the percentage content of vitamin B and/or vitamin B2 in the spray is 0.10-1.00%, and the percentage content of vitamin E in the spray is 0.50-1.00%.
7. The sole repair and moisture retention spray containing hyaluronic acid according to claim 5, wherein the mass ratio of vitamin C, vitamin B2 and vitamin E in the vitamin complex is 10:3:3: 4.
8. The sole repair moisturizing spray containing hyaluronic acid as claimed in claim 1, wherein the amino acid is 1.50% by mass.
9. The hyaluronic acid-containing plantar repair moisturizing spray according to claim 1, wherein the amino acid component is at least one selected from glycine, proline and histidine.
10. The hyaluronic acid-containing plantar repair moisturizing spray according to claim 9, wherein the amino acid component is an amino acid complex consisting of glycine, proline and histidine.
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| CN112438905A (en) * | 2020-12-03 | 2021-03-05 | 澳思美日用化工（广州）有限公司 | Moisturizing and skin-brightening composition and application thereof |
| CN114652648A (en) * | 2022-04-01 | 2022-06-24 | 深圳津梁生活科技有限公司 | Novel freeze-dried mask and preparation method thereof |
| WO2024199347A1 (en) * | 2023-03-31 | 2024-10-03 | 华熙生物科技股份有限公司 | Anti-inflammatory composition and use thereof |
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