CN110973019B - Method for promoting survival and growth of takifugu rubripes from fertilized eggs to larvae and juvenile fish by using LED light source - Google Patents

Abstract

The invention relates to a method for promoting survival and growth of takifugu rubripes from fertilized eggs to larvae and juvenile fishes by using an LED light source, and belongs to the technical field of industrial culture. The method comprises the steps of arranging an LED light source on the water surface; in the cultivation process of fertilized eggs of the takifugu rubripes, the LED light source is full spectrum; in the process of cultivating the larvae and the young fishes of the takifugu rubripes, the LED light source is as follows: green at 525-530nm or yellow at 590-595 nm. According to the invention, the survival rate of the fries is improved and the growth of the fries is promoted by regulating and controlling the illumination parameters during the fry cultivation period, so that the production efficiency and the economic benefit of the industrial fry cultivation of the takifugu rubripes can be improved.

Description

A method for promoting the survival and growth of redfin puffer fish from fertilized eggs to juveniles using LED light sources

technical field

The invention relates to a method for promoting the survival and growth of red-finned pufferfish from fertilized eggs to juveniles by using an LED light source, and belongs to the technical field of factory farming.

Background technique

Identifying the influence of important environmental and biological factors on fish survival, growth and development is the core content of optimizing factory farming technology. Light is one of the important environmental factors, and it has an important impact on fish at all stages from embryonic development to sexual maturity. Illumination includes three elements: spectrum, light intensity and photoperiod. Studies have shown that most marine fish feed and communicate mainly through vision. Therefore, the lighting conditions used in aquaculture production should increase the visual range of fish, shorten the feeding time, and ultimately promote their growth and survival.

Due to the abundance of suspended particles and phytoplankton in nearshore waters, the spectral composition of incident light often changes dramatically. As the depth of the seawater increases, the incident light usually decays rapidly, with blue light at 470 nm having the strongest penetration in seawater, while red and ultraviolet light are quickly absorbed when penetrating seawater. The light environment of fish living at different depths is different, resulting in the preference for light environment in the process of fish factory farming.

The red-finned puffer belongs to the order Pufferfish and belongs to the genus Oriental puffer. It is mainly distributed in the coastal areas of my country, the Korean Peninsula and the coastal areas of Japan. It has delicious meat, tender taste and rich nutrition. It is very popular in the Japanese and Korean markets. It is the most valuable kind of pufferfish. As the largest exporter of pufferfish, China exports only 2,000-3,000 tons of redfin puffer to Japan and South Korea every year. Since the promulgation of the Notice on Conditional Opening of the Processing and Operation of Breeding Red-fin puffer and Breeding Dark-pattern Puffer in September 2016, the domestic puffer market has also gradually opened up. In northern my country, the red-finned puffer has become the first choice for industrial mariculture and an advantageous aquatic product for exporting foreign exchange. At present, the pufferfish fry are basically derived from artificial breeding in factories. In the current breeding process, the environmental factors that are mainly concerned include: ammonia nitrogen, nitrite, salinity and water temperature, while the lack of suitable light environment during the breeding process Research and Technology Application. In view of the low rate of membrane rupture of fertilized eggs and the common death of juveniles and juveniles in the breeding and production of redfin pufferfish, the establishment of a simple and convenient light environment regulation technology can improve the early hatching rate of seedlings and seedlings. Growth quality is important.

SUMMARY OF THE INVENTION

According to the preference of the red-finned puffer fish on the light environment in the early stage, the invention can promote the hatching of the fertilized eggs of the red-finned puffer fish and the survival and growth of the juvenile fish by adjusting the spectrum, photoperiod and light intensity of the LED light source, and solve the above problems. The problem.

The invention provides a method for promoting the survival and growth of red-finned puffer fish from fertilized eggs to juveniles by using an LED light source. The method is to set an LED light source on the water surface; , the LED light source is a full spectrum; in the process of cultivating juvenile red-fin puffer fish, the LED light source is: 525-530nm green light or 590-595nm yellow light.

In the present invention, the photoperiod is preferably 24 hours of light in the process of cultivating the fertilized eggs of the red-finned puffer fish; in the process of cultivating the juveniles of the red-finned puffer fish, the photoperiod is 12 hours of light, and the rest is darkness.

In the present invention, preferably, the light intensity of the LED light source is 0.5 W/m ² .

In the present invention, the LED light source is preferably arranged at a position of 60-100 cm on the water surface.

The beneficial effects of the present invention are:

The invention artificially creates a light environment suitable for the early life of the seedlings according to the preference of the fertilized eggs and the early juveniles of the red-finned puffer fish to a specific light environment, thereby improving the hatching rate of the fertilized eggs, the growth rate and the survival rate of the juveniles and juveniles. , increase the welfare of farmed fish, improve the efficiency and economic benefits of factory farming.

The present invention improves the survival rate of the seedlings and promotes their growth by regulating the light parameters during the seedling cultivation, thereby improving the production efficiency and economic benefit of the industrialized seedling cultivation of the red-finned pufferfish.

Detailed ways

The following non-limiting examples may enable those of ordinary skill in the art to more fully understand the present invention, but do not limit the present invention in any way.

Example 1

In the Zhuanghe Redfin puffer factory breeding base in Liaoning Province, during the breeding process of the fertilized eggs of the redfin puffer: the hatching water temperature of the fertilized eggs is 19-19.5℃, the water depth is 55cm, and a full-spectrum LED light source is hung 100cm above the water surface. , the photoperiod is light for 24h, the light intensity is 0.5W/m ² , fertilized eggs are obtained by wet artificial insemination, washed with sea water for many times to remove sperm and blood clots, and then 25g of washed fertilized eggs are taken out with a net bag, and cultivated as usual The method is placed in a breeding bucket, inflated until the fertilized eggs float in the water, and the water is changed every 2 hours during the day and every 4 hours at night. The amount of water exchange is 100-200% each time. After 5 days of incubation, the red-finned puffer began to hatch and break the membrane, and on the 4th day after hatching, the red-finned pufferfish began to hatch. The basic hatching of Oriental Pufferfish has been completed, the hatching rate has reached 45%, and the hatching volume has reached 42/L;

During the cultivation process of juvenile red-finned puffer fish, a green LED light source of 525-530nm was hung 100cm above the water surface, and the photoperiod was 12h. Light: 12h dark, light intensity was 0.5W/m ² After the opening of the larvae above /2, they were fed with rotifers fortified with fish oil and vitamins. The density of rotifers gradually increased with the growth of the red-finned pufferfish. On the 10th day after hatching, the same nutritionally fortified Artemia was started to be fed at the same time. Artemia nauplii, the density of Artemia nauplii also gradually increased with the growth of red-finned pufferfish. On the 18th day after hatching, the feeding of rotifers was stopped, and on the 19th day after hatching, the feeding of thawed large brine meat was started at the same time. The feeding amount and feeding frequency of brine meat also gradually increased with the growth of red-fin pufferfish. On the 24th day after hatching, feeding Artemia nauplii was stopped, and at the same time, crushed shrimp skin was started. The feeding amount of shrimp skin And the number of feedings also gradually increased with the growth of the redfin pufferfish. On the 26th day after hatching, the feeding of compound feed was started at the same time, and the feeding amount of large braised meat and shrimp skin was reduced, and the feeding was stopped on the 40th day after hatching. When the juvenile red-finned puffer fish reaches 6cm in length, the amount of daily water exchange gradually increases with the growth of the juvenile red-finned puffer fish, so as to maintain pH, salinity, and The total ammonia nitrogen and nitrite concentrations were maintained within the normal range. According to the above culture process, the survival rate of larvae and juveniles reached 63%, and the specific growth rate reached 6.5% (from 5mm larvae to 6cm juvenile stage).

Example 2

The difference from Example 1 is that the 525-530 nm green LED light source is replaced with a 590-595 nm yellow LED light source.

Claims (1)

1. a method for promoting the survival and growth of the red-finned puffer fish from fertilized egg to juvenile juvenile fish with LED light source, it is characterized in that: described method is water depth 55cm, and LED light source is set at 100cm place on water surface, and described LED The light intensity of the light source is 0.5W/m ² ; In the process of cultivating the fertilized eggs of the red-finned pufferfish, the LED light source is full spectrum, and the photoperiod is 24h of illumination; During the cultivation of juvenile red-finned puffer fish, the LED light source was green light at 525-530 nm, the photoperiod was light for 12 hours, and the rest was dark, until the full length of the juvenile red-finned puffer fish reached 6 cm.