Disclosure of Invention
The invention mainly aims to provide automatic microorganism inspection equipment, which aims to solve the technical problem that the operation of taking out or placing a culture dish is long in time consumption in the automatic microorganism inspection equipment in the prior art.
In order to achieve the above object, the present invention provides an automatic microorganism testing apparatus comprising: the device comprises a cabin body, a sample injection area, a culture area, an observation area and a sample outlet area are formed in the cabin body, and the culture area is used for storing culture dishes; a first temperature adjusting device arranged in the culture zone and used for adjusting the temperature in the culture zone; the imaging device is arranged in the observation area and is used for acquiring images of the culture dish; the sample injection mechanism is arranged in the sample injection area and used for conveying the culture dish to the sample injection area; the sample outlet mechanism is arranged in the sample outlet area and used for taking the culture dish away from the sample outlet area; the lifting basket is movably arranged in the cabin body and is used for bearing the culture dish; the first dish taking mechanism is arranged in the cabin body and used for placing the culture dish in the sample entering area in the lifting basket in the sample entering area; the second dish taking mechanism is arranged in the cabin body and is used for moving the lifting basket at least in the sample introduction area, the culture area and the observation area; and the third dish taking mechanism is arranged in the cabin body and used for moving the culture dish in the basket of the observation area to the imaging device and moving the culture dish with the acquired image to the sample outlet area.
In one embodiment, the sample injection mechanism comprises: the sample introduction storage basket is used for stacking culture dishes, a sample introduction stacking port is formed in the top of the sample introduction storage basket, and a sample introduction port and an operation hole are formed in the bottom of the sample introduction storage basket; the sample injection operation push claw is movably arranged along the horizontal direction and pushes the culture dish positioned at the bottom of the sample injection storage basket out of the sample injection port through the operation hole; and the sample injection driving part is in driving connection with the sample injection operation push claw and is used for driving the sample injection operation push claw to move.
In one embodiment, the sample injection mechanism further comprises a sample injection slide plate connected to the sample injection port for transporting the culture dish from the sample injection port to the sample injection region.
In one embodiment, the sampling mechanism comprises: the sample outlet storage basket is used for stacking culture dishes, and the top of the sample outlet storage basket is provided with a sample outlet stacking opening; the liftable bottom plate is arranged in the sample storage basket in a liftable manner; the lifting driving piece drives the lifting bottom plate to lift; the sample outlet operation push claw is movably arranged in the horizontal direction and used for pushing the culture dish into the sample outlet storage basket from the sample outlet stacking port to form a sample outlet driving piece, is in driving connection with the sample outlet operation push claw and is used for driving the sample outlet operation push claw to move.
In one embodiment, the sample discharging mechanism further comprises a sample discharging sliding plate, and the sample discharging sliding plate is connected with the sample discharging and stacking port and used for conveying the culture dish from the sample discharging area to the sample discharging and stacking port.
In one embodiment, the sample outlet sliding plate is provided with an avoiding groove for avoiding the sample outlet operation pushing claw.
In one embodiment, the first dish taking mechanism comprises: the first Y-axis guide rail is arranged in the sample injection area; the first Z-axis guide rail is movably arranged on the first Y-axis guide rail; a first X-axis guide rail movably mounted on the first Z-axis guide rail; the first dish taking claw is movably arranged on the first X-axis guide rail and used for grabbing and placing a culture dish.
In one embodiment, the second dish taking mechanism comprises: the second X-axis guide rail is installed across the sample injection area, the culture area, the observation area and the culture area; the second Z-axis guide rail is movably arranged on the second X-axis guide rail; the second Y-axis guide rail is movably arranged on the second Z-axis guide rail and transversely spans the sample injection area and the observation area; and the second dish taking claw is movably arranged on the second Y-axis guide rail and is used for grabbing and placing a culture dish.
In one embodiment, the third dish taking mechanism comprises: the third X-axis guide rail is arranged in the observation area; a third Z-axis guide rail movably mounted on the third X-axis guide rail; the third Y-axis guide rail is movably arranged on the third Z-axis guide rail and spans the observation area and the sample outlet area; and the third dish taking claw is movably arranged on the third Y-axis guide rail and is used for grabbing and placing a culture dish.
In one embodiment, the automatic microorganism testing apparatus further comprises: the second temperature adjusting device is arranged in the observation area and used for adjusting the temperature in the observation area; and the controller is electrically connected with the first temperature regulating device and the second temperature regulating device respectively and is used for controlling the temperature of the observation area to be the same as that of the culture area.
By applying the technical scheme of the invention, when in use, a culture dish to be cultured and detected is directly placed into the sample introduction mechanism, the sample introduction mechanism can convey the culture dish to the sample introduction area, then the first dish taking mechanism can place the culture dish in the sample introduction area in the basket positioned in the sample introduction area, and the second dish taking mechanism can move the basket to the culture area for culture. After culturing for a period of time, the second dish taking mechanism moves the basket to the observation area, the third dish taking mechanism moves the culture dish in the basket of the observation area to the imaging device for image acquisition, if the culture dish needs to be cultured and image acquisition continuously, the culture dish is placed back into the basket of the observation area, and the basket is moved to the culture area by the second dish taking mechanism for continuous culture; if the culture dish has completed all the cultivation and image acquisition works, the culture dish in the basket of the observation area is moved to the sample outlet area by the third sample taking mechanism, and the sample outlet mechanism takes away the culture dish from the sample outlet area. Finally, the operator only needs to take all the detected culture dishes away from the sample outlet mechanism. Therefore, the automatic culture dish placing and taking out is realized, operators can conveniently and quickly take out or place the culture dish from the automatic microorganism inspection equipment, and the working efficiency is improved; in addition, operating personnel can also close the cabin body faster, reduce the influence to the internal temperature of cabin, guarantee experiment validity.
In addition to the objects, features and advantages described above, other objects, features and advantages of the present invention are also provided. The present invention will be described in further detail below with reference to the drawings.
Detailed Description
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict. The present invention will be described in detail below with reference to the embodiments with reference to the attached drawings.
In order to make the technical solutions of the present invention better understood, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the terms "first," "second," and the like in the description and claims of the present invention and in the drawings described above are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the terms so used are interchangeable under appropriate circumstances for describing embodiments of the invention herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
Fig. 1, 2 and 3 show an embodiment of the automatic microorganism testing apparatus of the present invention, which includes a chamber 10, an imaging device 20, a sample introduction mechanism 30, a sample discharge mechanism 40, a basket 50, a first dish taking mechanism 60, a second dish taking mechanism 70 and a third dish taking mechanism 80. A sample injection area a, a culture area b, an observation area c and a sample outlet area d are formed in the chamber body 10, and the culture area b is used for storing culture dishes. The first temperature adjusting means is provided in the culture section b for adjusting the temperature in the culture section b. The imaging device 20 is disposed in the observation area c for image acquisition of the culture dish. The sample injection mechanism 30 is arranged in the sample injection region a and is used for conveying the culture dish to the sample injection region a. The sample outlet mechanism 40 is arranged in the sample outlet area d and is used for removing the culture dish from the sample outlet area d. The basket 50 is movably disposed within the chamber 10 for carrying culture dishes. A first dish taking mechanism 60 is provided in the nacelle 10 for placing a culture dish of the sample introduction area a in the basket 50 located at the sample introduction area a. The second dish taking mechanism 70 is disposed in the chamber 10 for moving the basket 50 at least in the sample introduction region a, the culture region b and the observation region c. The third dish taking mechanism 80 is disposed in the cabin 10, and is configured to move the culture dish in the basket 50 of the observation area c to the imaging device 20, and also configured to move the culture dish with the captured image to the sample outlet area d.
By applying the technical scheme of the invention, when in use, a culture dish to be cultured and detected is directly placed into the sample injection mechanism 30, the sample injection mechanism 30 can convey the culture dish to the sample injection region a, then the first dish taking mechanism 60 can place the culture dish of the sample injection region a in the basket 50 positioned in the sample injection region a, and the second dish taking mechanism 70 can move the basket 50 to the culture region b for culture. After culturing for a period of time, the second dish taking mechanism 70 moves the basket 50 to the observation area c, the third dish taking mechanism 80 moves the culture dish in the basket 50 of the observation area c to the imaging device 20 for image acquisition, if the culture dish needs to continue culturing and image acquisition, the culture dish is put back into the basket 50 of the observation area c, and the second dish taking mechanism 70 moves the basket 50 to the culture area b for continuous culturing; if the culture dish completes all the culture and image collection work, the third dish taking mechanism 80 moves the culture dish in the basket 50 of the observation area c to the sample outlet area d, and the sample outlet mechanism 40 takes the culture dish from the sample outlet area d. Finally, the operator only needs to remove all the tested culture dishes from the sample outlet mechanism 40. Therefore, the automatic culture dish placing and taking out is realized, operators can conveniently and quickly take out or place the culture dish from the automatic microorganism inspection equipment, and the working efficiency is improved; in addition, the operator can also close the cabin 10 quickly, reducing the influence on the temperature in the cabin 10 and ensuring the effectiveness of the experiment.
As shown in the figure, in the technical solution of the present embodiment, the cabin 10 includes a bottom cabin and a cabin door openably disposed on the bottom cabin, the imaging device 20, the dish taking mechanism, the temperature adjusting device and the controller are disposed on the bottom cabin, and the cabin door can be opened to take and place the culture dish.
As shown in fig. 4 and fig. 5, in the technical scheme of this embodiment, the sample feeding mechanism 30 includes a sample feeding storage basket 31, a sample feeding operation push claw 32 and a sample feeding driving member 33, the sample feeding storage basket 31 is used for stacking culture dishes, a sample feeding stacking port 311 is disposed at the top of the sample feeding storage basket 31, a sample feeding port 312 and an operation hole 313 are disposed at the bottom of the sample feeding storage basket 31, the sample feeding operation push claw 32 is movably disposed along the horizontal direction, and the culture dish located at the bottom of the sample feeding storage basket 31 is pushed out from the sample feeding port 312 through the operation hole 313. The sample introduction driving part 33 is in driving connection with the sample introduction operation pushing claw 32 and is used for driving the sample introduction operation pushing claw 32 to move. When the sample feeding device is used, the culture dishes are sequentially stacked in the sample storage basket 31 from the sample stacking port 311, the sample feeding driving member 33 drives the sample feeding operation push claw 32 to move each time, the culture dish positioned at the bottommost part of the sample storage basket 31 can be pushed out from the sample inlet 312, and the culture dish positioned above the culture dish falls down to wait for the push claw 32 to push out the culture dish from the sample inlet 312 again.
Optionally, in the technical solution of this embodiment, the sample injection driving component 33 is composed of a motor, a screw and nut mechanism, the motor and the nut mechanism are connected and fixedly disposed, the screw is connected to the sample injection operation push claw 32, and the screw drives the sample injection operation push claw 32 to move under the driving of the nut mechanism.
More preferably, in the technical solution of this embodiment, the sample injection mechanism 30 further includes a sample injection slide 34, and the sample injection slide 34 is connected to the sample injection port 312 and is used for conveying the culture dish from the sample injection port 312 to the sample injection region a. The culture dish can be more smoothly conveyed to the sample introduction area a through the sample introduction sliding plate 34.
As shown in fig. 6 and 7, in the technical solution of this embodiment, the sample discharging mechanism 40 includes a sample discharging storage basket 41, a liftable bottom plate 42, a lifting driving member 43, a sample discharging operation pushing claw 44 and a sample discharging driving member 45, the sample discharging storage basket 41 is used for stacking culture dishes, a sample discharging stacking opening 411 is formed in the top of the sample discharging storage basket 41, the liftable bottom plate 42 is arranged in the sample discharging storage basket 41 in a liftable manner, the lifting driving member 43 drives the liftable bottom plate 42 to ascend and descend, the sample discharging operation pushing claw 44 is movably arranged along the horizontal direction and is used for pushing the culture dishes into the sample discharging storage basket 41 from the sample discharging stacking opening 411, and the sample discharging driving member 45 is in driving connection with the sample discharging operation pushing claw 44 and is used for driving the sample discharging operation pushing claw 44 to move. When the culture dish rack is used, as shown in fig. 3, when the third dish taking mechanism 80 moves the culture dish in the basket 50 in the observation area c to the circular dotted line part of the sample outlet area d, the sample outlet driving member 45 drives the sample outlet operation push claw 44 to move, so as to push the culture dish to move from the sample outlet stacking opening 411 to the liftable bottom plate 42 in the sample outlet storage basket 41, then the liftable bottom plate 42 is driven by the lifting driving member 43 to descend for a certain position, and the sample outlet driving member 45 drives the sample outlet operation push claw 44 to push another culture dish to stack on the culture dish until the sample outlet storage basket 41 is stacked with the culture dish.
Optionally, the sample output driving member 45 is also composed of a motor and a screw nut mechanism, the motor and the nut member are connected and fixedly arranged, the screw member is connected with the sample output operation push claw 44, and the screw member drives the sample output operation push claw 44 to move under the driving of the nut member. Alternatively, the elevation driving member 43 may employ an electric cylinder or a linear motor.
More preferably, the discharging mechanism 40 further comprises a discharging slide plate 46, and the discharging slide plate 46 is connected to the discharging stacking port 411 for conveying the culture dish from the discharging area d to the discharging stacking port 411. The petri dish can be moved from the sample outlet area d to the sample outlet storage basket 41 more smoothly by the sample outlet slide plate 46. Correspondingly, as shown in fig. 6, the sample discharging slide plate 46 is provided with an avoiding groove 461 for avoiding the sample discharging operation pushing claw 44, so as to prevent the sample discharging slide plate 46 from influencing the movement of the sample discharging operation pushing claw 44.
As shown in fig. 1 and 2, in the present embodiment, the first dish taking mechanism 60 includes a first Y-axis guide 61, a first Z-axis guide 62, a first X-axis guide 63, and a first dish taking claw 64, the first Y-axis guide 61 is installed in the sample introduction area a, the first Z-axis guide 62 is movably installed on the first Y-axis guide 61, the first X-axis guide 63 is movably installed on the first Z-axis guide 62, the first dish taking claw 64 is movably installed on the first X-axis guide 63, and the first dish taking claw 64 is used for holding a culture dish. When the dish taking mechanism is used, the first dish taking claw 64 moves on the first X-axis guide rail 63 along the X-axis direction, the first X-axis guide rail 63 moves on the first Z-axis guide rail 62 along the Z-axis direction, and the first Z-axis guide rail 62 moves on the first Y-axis guide rail 61 along the Y-axis direction, so that the first dish taking claw 64 can move freely in the space where the first dish taking mechanism 60 is located.
Optionally, the second dish taking mechanism 70 includes a second X-axis guide rail 71, a second Z-axis guide rail 72, a second Y-axis guide rail 73, and a second dish taking claw 74, the second X-axis guide rail 71 spans across the sample injection region a and the culture region b, and spans across the observation region c and the culture region b, the second Z-axis guide rail 72 is movably mounted on the second X-axis guide rail 71, the second Y-axis guide rail 73 is movably mounted on the second Z-axis guide rail 72, the second Y-axis guide rail 73 spans across the sample injection region a and the observation region c, the second dish taking claw 74 is movably mounted on the second Y-axis guide rail 73, and the second dish taking claw 74 is used for holding a culture dish. When the dish taking mechanism is used, the second dish taking claw 74 moves on the second Y-axis guide rail 73 along the Y axis, the second Y-axis guide rail 73 moves on the second Z-axis guide rail 72 along the Z axis, and the second Z-axis guide rail 72 moves on the second X-axis guide rail 71 along the X axis, so that the second dish taking claw 74 can freely move in the space where the second dish taking mechanism 70 is located. Preferably, as shown in fig. 2, in the technical solution of the present embodiment, two second X-axis guide rails 71 and two second Z-axis guide rails 72 are respectively arranged in parallel, and the second dish taking mechanism 70 is similar to a gantry crane structure, so that the basket 50 is more stable to be grabbed and placed.
Optionally, the third dish taking mechanism 80 includes a third X-axis guide rail 83, a third Z-axis guide rail 82, a third Y-axis guide rail 81 and a third dish taking claw 84, the third X-axis guide rail 83 is installed in the observation area c, the third Z-axis guide rail 82 is movably installed on the third X-axis guide rail 83, the third Y-axis guide rail 81 is movably installed on the third Z-axis guide rail 82 and spans the observation area c and the sample outlet area d, the third dish taking claw 84 is movably installed on the third Y-axis guide rail 81, and the third dish taking claw 84 is used for holding a culture dish. In use, the third dish taking claw 84 moves along the Y axis on the third Y-axis guide rail 81, the third Y-axis guide rail 81 moves along the Z axis on the third Z-axis guide rail 82, and the third Z-axis guide rail 82 moves along the X axis on the third X-axis guide rail 83, so that the third dish taking claw 84 can move freely in the space where the third dish taking mechanism 80 is located.
As an alternative, in the solution of this embodiment, the top of the basket 50 has a magnetic component, and the above-mentioned dish taking claw of the deployment basket 50 may be an electromagnet cooperating with the magnetic component.
More preferably, in the technical solution of this embodiment, the automatic microorganism testing apparatus further includes a second temperature adjusting device disposed in the observation area c for adjusting the temperature in the observation area c, and a controller electrically connected to the first temperature adjusting device and the second temperature adjusting device, respectively, for controlling the temperature of the observation area c to be the same as the temperature of the culture area b. Thus, the temperature of the observation area c can be controlled to be the same as the temperature of the culture area b, and the abnormal production of microorganisms in the culture dish caused by the abnormal temperature in the observation area c can be avoided. In addition, another important reason is that when the temperatures in the culture region b and the observation region c are different, once the culture dish at a low temperature takes the observation region c at a higher temperature, the culture dish may be fogged or even condensed with water drops, and the image information acquired by the imaging device 20 in the subsequent image calculation process easily calculates the water drops or water fog as bacterial colonies, so that errors are easily generated and the effectiveness of the experiment is reduced. According to the technical scheme, the temperature of the observation area c is controlled to be the same as that of the culture area b, and the culture dish is transferred in different areas with the same temperature, so that the phenomenon that the culture dish is fogged or condensed water drops can be avoided, and the experimental effectiveness is guaranteed.
In the technical scheme of this embodiment, temperature regulation apparatus includes cooling element and heating element, and cooling element is used for refrigerating, and heating element is used for heating. When the device is used, the culture space can be refrigerated through the refrigerating element so as to reduce the temperature of the culture space, the culture space can be heated through the heating element so as to increase the temperature of the culture space, and the temperature of the culture space can be maintained at the temperature meeting the requirement of microorganism culture through the two components. As an alternative, the refrigeration element is a semiconductor refrigeration element, and specifically, a peltier element can be selected to be matched with the fan. Optionally, the heating element is an electric heating element, and the electric heating element may select an electric heating tube or a heat radiation element to heat.
The relative arrangement of the components and steps, the numerical expressions and numerical values set forth in these embodiments do not limit the scope of the present invention unless specifically stated otherwise. Meanwhile, it should be understood that the sizes of the respective portions shown in the drawings are not drawn in an actual proportional relationship for the convenience of description. Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate. In all examples shown and discussed herein, any particular value should be construed as merely illustrative, and not limiting. Thus, other examples of the exemplary embodiments may have different values. It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, further discussion thereof is not required in subsequent figures.
Spatially relative terms, such as "above … …," "above … …," "above … …," "above," and the like, may be used herein for ease of description to describe one device or feature's spatial relationship to another device or feature as illustrated in the figures. It will be understood that the spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures. For example, if a device in the figures is turned over, devices described as "above" or "on" other devices or configurations would then be oriented "below" or "under" the other devices or configurations. Thus, the exemplary term "above … …" can include both an orientation of "above … …" and "below … …". The device may be otherwise variously oriented (rotated 90 degrees or at other orientations) and the spatially relative descriptors used herein interpreted accordingly.
In the description of the present invention, it is to be understood that the orientation or positional relationship indicated by the orientation words such as "front, rear, upper, lower, left, right", "lateral, vertical, horizontal" and "top, bottom", etc. are usually based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and in the case of not making a reverse description, these orientation words do not indicate and imply that the device or element being referred to must have a specific orientation or be constructed and operated in a specific orientation, and therefore, should not be considered as limiting the scope of the present invention; the terms "inner and outer" refer to the inner and outer relative to the profile of the respective component itself.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.