CN110878252B - Culture cylinder, culture system and culture method for microorganism culture - Google Patents

Abstract

The application relates to the technical field of microorganism culture, in particular to a culture cylinder, a culture system and a culture method for microorganism culture, wherein the culture cylinder comprises a cylinder body with an open upper end and a cover body matched with the open upper end of the cylinder body, a culture medium cavity and a gas cavity are formed in the inner space of the cylinder body from bottom to top, the culture medium cavity is used for containing culture medium suitable for microorganism growth, the gas cavity is used for containing suitable gas suitable for microorganism growth, and a gas replacement device is further arranged in the cylinder body and is used for replacing the gas in the gas cavity with new suitable gas. The culture cylinder reduces the risk of death or variation or pollution in the growth and development processes of microorganisms, ensures good development speed of the microorganisms and saves culture time.

Description

A culture tube, culture system and culture method for microorganism culture

technical field

The invention relates to the technical field of microorganism cultivation, in particular to a cultivation cylinder, a cultivation system and a cultivation method for microorganism cultivation.

Background technique

For microorganisms, it is a large group of organisms including bacteria, viruses, fungi, and some small protozoa, which widely exist in the biological world. Although the microorganisms are small, they are closely related to human life. It is widely involved in many fields such as medicine and health, industry and agriculture, and environmental protection.

Over the years, microbiologists have carried out scientific experiments in various aspects such as classification, physiology, and genetics, studied the physiological and biochemical characteristics of microorganisms, and summed up a set of microbial detection, screening and cultivation methods, so that microorganisms can be cultivated according to people's needs. People need services.

The current microbial cultivation usually adopts the following steps:

The first is to collect microbial specimens, and then pretreat the collected microbial specimen materials to reduce the impurity content in the specimen materials, then carry out enrichment culture to obtain a large number of microorganisms, and then select microorganisms to obtain primary strains , and then conduct a performance test on the primary strains to judge whether the performance of the selected strains can meet the requirements for inoculation and cultivation. If the strains meet the requirements for inoculation and cultivation, they are qualified strains. After the qualified strains are obtained, the strains are preserved, so that the life activities of the microorganisms are in a semi-permanent dormant state, and the corresponding strains are revived for use when needed.

Although the above-mentioned method has been widely used in the current field of microbial application technology, in further research, the inventor found that the current method still has deficiencies, as follows:

Although microbial preservation can achieve long-term preservation and ensure the convenience of use, there are still shortcomings: on the one hand, bacterial strain preservation requires a lot of cost. In the current microbial application field, the cost of bacterial strain preservation occupies a very large Large cost ratio; on the other hand, in the process of strain preservation, the preservation temperature, sealing quality and gas dryness all affect the preservation quality. When these conditions change, the strain may not die, mutate or be contaminated ; On the other hand, the recovery of the strains also requires a large cost, and in the process of recovery of the strains, there are also risks of bacterial death and contamination.

Therefore, it is currently necessary to design a microbial culture system that can avoid the high cost of strain preservation and recovery procedures, and the risk of strain death and contamination.

Contents of the invention

The purpose of the present invention is to provide a method that can avoid the cost caused by the preservation and recovery process of bacteria in view of the high cost caused by the preservation and recovery process of the bacteria in the current microbial culture, and the risk of death and contamination of the bacteria. Microbial culture systems with high risk of strain death and contamination.

In order to realize the above-mentioned purpose of the invention, the present invention provides the following technical solutions:

A culture cylinder for microbial cultivation, comprising a cylinder with a closed lower end and an open upper end and a cover that matches the open end of the upper end of the cylinder, and the internal space of the cylinder is divided into culture substrates from bottom to top chamber and gas chamber, the culture substrate chamber is used to accommodate the culture medium suitable for microbial growth, the gas chamber is used to accommodate suitable gas suitable for microbial growth, a gas replacement device is also arranged in the cylinder, and the gas replacement The device is used to replace the gas in the gas cavity with new suitable gas.

In the scheme of this application, the culture medium suitable for microorganism cultivation is the current conventional culture medium, which can be a liquid medium, or a solid medium or a colloidal medium. For different types of microorganisms, the formula of the medium Different from the morphology, for different types of microorganisms, the gas formula suitable for the reproduction and growth of microorganisms may also be different. The formula, shape of the medium, and the gas suitable for microorganisms are those skilled in the art based on the conventional techniques in the field mastered by those skilled in the art. Different types of microorganisms can be selected or produced to obtain suitable medium and gas suitable for such microorganisms, so details will not be repeated in this content.

In the current cultivation of microorganisms, the periodic gas composition is the key factor affecting the growth and development of microorganisms. When the surrounding gas composition changes greatly, the reproduction, growth and development of microorganisms will be affected, and even cause the death or mutation of microorganisms in severe cases. , or there may be problems such as pollution, which is also the key purpose of the current strain preservation. In actual work, the inventor found that during the growth and reproduction of microorganisms, whether they are aerobic microorganisms or anaerobic microorganisms, most of them will It affects the air around it. The growth and development of some microorganisms will absorb some components in the surrounding gas, and some microorganisms will release some gases. Some microorganisms absorb certain gases and emit some gases at the same time. Therefore, as As time goes by, the growth of microorganisms progresses, and the surrounding gas will also change accordingly, which leads to the risk of death, mutation and contamination due to changes in gas composition during the growth and development of microorganisms. Based on the above, in the scheme of the present application, the microorganisms are cultured in the culture medium in the cylinder, and the gas chamber contains suitable gas. The advanced gas replacement device replaces the gas in the gas chamber with a new suitable gas, thus reducing the risk of death or mutation or contamination during the growth and reproduction of microorganisms, and also ensuring a good development speed of microorganisms and saving cultivation time .

The present application also discloses a gas replacement device for the above-mentioned culture tube, the gas replacement device includes a replacement component and a gas discharge component, and the gas discharge component is used to communicate the gas cavity with the external space of the cylinder body, so The replacement component is used to push the gas in the gas cavity, so that the gas in the gas cavity is discharged out of the cylinder by the gas discharge component.

As a preferred technical solution, the gas discharge assembly includes a communication pipe, one end of which communicates with the gas cavity, and the other end passes through the cylinder body and communicates with the external space of the cylinder body.

As a preferred technical solution, a shut-off valve is also provided on the communicating pipe. The communication and blocking states of the communication pipe are controlled by opening and closing of the cut-off valve.

As another preferred technical solution, a one-way conducting device is also provided on the communicating pipe, so that the communicating pipe is conducted in one direction from the inside of the cylinder to the outside of the cylinder.

As a preferred technical solution, the one-way conduction device is arranged at an end of the communicating pipe close to the gas cavity.

As a preferred technical solution, the one-way passage device is a one-way valve.

As a preferred technical solution, the communication pipe is arranged vertically along the inner wall of the gas chamber, the lower end of the communication pipe is in clearance with the upper edge of the culture medium chamber, and the upper end of the communication pipe passes through the The upper end of the barrel forms an exhaust nozzle.

As another preferred technical solution, the communication pipe includes a passing section and a vertical section, the vertical section is vertically arranged along the outer wall of the cylinder, one end of the passing section is located in the cylinder, and The upper edge of the culture substrate cavity is clearance-fitted, and the other end passes through the outside of the cylinder and is connected to the lower end of the vertical section.

As a further preferred technical solution, the passage section is inclined downward in the direction from the inside of the cylinder to the outside of the cylinder. The exit section is inclined downward, as mentioned above, when the exhaust gas condenses into droplet particles in the communication pipe, these particles can be prevented from falling into the culture substrate cavity, thereby ensuring good culture quality of microorganisms.

As a further preferred technical solution, a downwardly bent U-bend is connected between the passing-out section and the vertical section.

As a further preferred technical solution, a drain valve is also provided at the bottom of the U-shaped elbow. When needed, the drain valve can be opened to discharge the liquid and/or solid particles in the U-bend.

As a preferred technical solution, the replacement assembly includes a push plate and a push rod arranged on the upper side of the push plate, the push rod is vertically arranged and passes through the cover, and the push rod is connected with the push rod The lids are in a sliding and sealing fit, and the push plate is in a sliding and sealing fit with the side wall of the gas chamber.

As a preferred technical solution, when the communication pipe is located in the cylinder, a notch is provided on the push plate corresponding to the communication pipe, and the notch of the push plate is in a sliding and sealing fit with the communication pipe. .

As a preferred technical solution, a through hole penetrating through the push rod from top to bottom is provided inside the push rod, and the through hole runs through the push plate, and a detachable device for closing the push rod is provided at the upper end of the push rod. Plugging caps for through holes.

As a preferred technical solution, the lower side of the push plate is in the shape of a cone with an upward convex middle, and the through hole of the push rod is located at the top of the lower tapered surface of the push plate. The lower side of the push plate is set in the shape of a cone that protrudes upward in the middle. When the liquid medium enters the through hole and flows down to the lower side of the push plate, it can flow radially downward along the tapered surface, thereby improving The uniformity of dispersion is added to the culture medium.

As a further preferred technical solution, a plurality of diversion grooves along the direction of the generatrix are provided on the lower side of the push plate, and the circumference of each diversion groove is evenly distributed. The dispersion uniformity of the culture medium in the circumferential direction is further improved through the setting of the diversion groove.

As a further preferred technical solution, a spray head connected to the through hole is also provided on the lower side of the push plate corresponding to the through hole, and the spray head is used to spray the liquid culture substrate into a mist. When the culture substrate is liquid, the supplementary culture medium is sprayed into mist by setting the nozzle, and these mist liquid particles gradually settle in the culture substrate chamber to ensure the uniformity of dispersion and further ensure the culture quality of microorganisms.

As a preferred technical solution, an observation window is also provided on the side wall of the cylinder, and the observation window is arranged on the side wall of the cylinder corresponding to the gas chamber, and the edge of the observation window is connected The edges are sealed and matched, and the observation window is made of transparent materials. There is an opening on the cylinder, and an observation window is set, which is convenient for visual observation of the microorganisms and the conditions of each component in the cylinder, and is convenient for the operator to operate.

As a preferred technical solution, a barometer for monitoring the air pressure in the gas cavity is also provided on the cylinder. Through the setting of the barometer, it is convenient to control the air pressure in the gas chamber, and at the same time, it is also convenient to monitor the amount of supplemented gas.

The present application also discloses a microbial culture system, which includes the above-mentioned microbial culture tube, and also includes a temperature control cabin, which has a temperature control chamber capable of maintaining a stable temperature in the cavity, and all or The lower part is inside the temperature control chamber. In microbial culture, the temperature of the medium and the surrounding environment of the microorganism is also one of the important factors. Whether the temperature is suitable determines the quality of the growth and development of the microorganism. In actual microbial cultivation, some microorganisms will absorb heat, resulting in the temperature of the culture substrate Some microorganisms will release heat, leading to an increase in the temperature of the culture substrate. These changes in temperature often inhibit the further growth and development of microorganisms, and in severe cases, mutations and death may even occur.

Therefore, in the scheme of the present application, by setting the temperature control cabin, the temperature in the temperature control chamber can be controlled and adjusted, and the temperature in the chamber can be kept stable. In this way, the temperature in the culture cylinder, especially the temperature in the culture substrate chamber They are all at a temperature suitable for the growth, development and reproduction of microorganisms, thereby ensuring the good cultivation quality of microorganisms.

As a preferred technical solution, the temperature control cabin has a closed cavity filled with heat transfer material, and the temperature control cabin is also equipped with a heating and/or Or a cooling thermostat, on the upper side plate of the temperature control cabin, there is also a sunken cavity sunken into the cabin, the culture tube is placed in the sunken cavity, and the side wall of the sunken cavity is in contact with the Compatible with the outer wall of the culture cylinder. In this way, by setting the temperature adjustment device to an appropriate temperature, by setting the heat transfer material in the closed cavity, the heat transfer material can be water, oil and other substances that can achieve uniform heating of the concave cavity, or it can be In this way, the temperature in the concave cavity can be controlled, and then the temperature in the microbial culture cylinder can be controlled.

As a preferred technical solution, a flexible sealing gasket is further provided on the upper edge of the concave cavity, and the sealing gasket is used to seal the gap between the outer wall of the culture cylinder and the concave cavity. The setting of the gasket, on the one hand, ensures good heat transfer between the concave cavity and the cylinder, and on the other hand, improves the stability of the cylinder.

As a preferred technical solution, the gasket is a rubber gasket.

As a preferred technical solution, several sunken cavities are arranged on the temperature control cabin, and one culture cylinder is set in each sunken cavity. It can greatly improve the culture efficiency, and when cultivating the same number of microorganisms, it can greatly reduce the number of microorganisms in a single cylinder, while improving the quality of culture, it also greatly reduces the risk of cross-infection between microorganisms, and also utilizes the ability to detect microbial mutations control.

As a preferred technical solution, there is a distance between adjacent concave cavities, so that after the culture tubes are arranged in the concave cavities, a passage for people to pass is formed between the culture tubes. It is convenient for staff to monitor and operate each culture tube.

As a preferred technical solution, the microorganism cultivation system further includes a box body, the temperature control cabin and the culture cylinder are arranged in the box body, and the temperature control cabin and the box body are detachably connected. By setting the box, the integrity of the culture system is improved, the pollution of the culture cylinder is reduced, and the transportation is also convenient.

As a preferred technical solution, the box body is a container.

As a preferred technical solution, a gas chamber is also provided in the box, and the gas chamber is used to store gas suitable for the microorganisms to be cultured. The gas filling pipe that matches the hole. The gas chamber has a closed cavity in which new unused gas is stored. The air filling pipe is provided with an air filling nozzle that is also compatible with the push through hole, and the air filling nozzle is in a detachable sealing fit with the push rod. After the air in the gas chamber in the cultivation cylinder is exhausted, the air supply pipe is matched with the through hole of the push rod to replenish new suitable gas suitable for the growth and development of microorganisms into the gas chamber.

As a preferred technical solution, the gas chamber is arranged on the top of the box.

As a preferred technical solution, the gas chamber includes a lower side plate, and the edge of the lower side plate is in sliding and sealing fit with the side wall of the box body, and between the lower side plate and the top plate of the box body The gas storage space of the gas chamber is formed between them. The gas filling pipe is arranged on the lower side plate and communicates with the gas storage space of the gas chamber. Drive device for moving the board vertically. In this application, the driving device is a driving motor, which drives the lower side plate to move, and presses the gas in the gas chamber into the gas chamber of the culture cylinder to realize the filling of suitable gas.

As a preferred technical proposal, a waste gas chamber is also provided in the box, and the waste gas chamber communicates with the communication pipes of each of the culture tubes. Corresponding to certain microbial cultivation, the appropriate gas in the gas chamber may cause serious pollution or poisonous and harmful. For such toxic and harmful gases, in the solution of this application, the waste gas chamber is collected and then concentrated for harmless treatment.

The present application also discloses a method for cultivating microorganisms using the above-mentioned microorganism cultivation system. After obtaining the microorganism strains, the strains are cultivated in the microorganism cultivation system, and then the microorganism cultivation system cultivated with the microorganism strains is transported to the site of use, The microorganisms in the culture system are then taken out for on-site use.

The microbial cultivation method of the present application, compared with the traditional method, because the microbial cultivation system of the present application is used in the transportation link, it directly avoids the microbial strain preservation step and the recovery step in the traditional method, so, first of all, The cost of preservation and recovery is eliminated, and more importantly, the risk of microbial death and mutation in the traditional preservation and recovery process is avoided. Moreover, with the method of this application, after the microbial strain is cultivated, it directly enters the culture system The cultivation of microorganisms in the environment reduces the risk of microbial variation and improves the purity of microorganisms. Moreover, when transported to the use site for use, the microorganisms have excellent activity, and they have also grown to a certain number, which greatly shortens the time spent on site. The time period of use is like this, and the cost of use is also greatly reduced. The microorganism cultivation method of the present application is especially suitable for the situation where the use site needs to be used immediately or in a short period of time.

As a preferred technical solution, after obtaining the microbial strains, before the strains are cultivated in the microbial culture system, the time node experiment of gas replacement and culture substrate replenishment in the microbial culture system is carried out,

The gas replacement and culture medium filling time node experiment of the microbial culture system: the temperature control cabin is set to a temperature suitable for the growth, development and reproduction of microorganisms, and the amount of culture medium loaded in the culture cylinder and the amount of microbial strains inserted are based on the actual transportation process. Put the culture medium and strains into the culture medium chamber of the culture cylinder, then fill the gas chamber of the culture cylinder with suitable gas, start timing, record the time reading h0, and select microorganisms according to the actual microbial species and the requirements of the field of use. Mutation rate threshold and mortality threshold, and then proceed to the following steps:

Step 1: Monitor the microorganisms in the culture medium, when either the variation rate or the death rate of the microorganisms in the culture cylinder reaches 90-95% of the threshold value, record the time reading h1,

Step 2: When detecting h1, the composition of the culture substrate and the ratio of each ingredient are compared, and the ratio of the ingredients is compared with the ratio of the original ingredients of the culture substrate, and the amount of components that need to be supplemented d1 is calculated.

Step 3: Add the added component amount d1 into the culture substrate cavity, and then replace the gas in the gas cavity with a new suitable gas,

Step 4: Continue to monitor the variation rate and mortality of microorganisms. When any of the variation rate and mortality of microorganisms in the culture cylinder reaches 90-95% of the threshold, record the time reading h2,

Step 5: When detecting h2, the composition of the culture substrate and the ratio of each ingredient are compared, and the ratio of the ingredients is compared with the ratio of the original composition of the culture substrate to calculate the amount of components that need to be supplemented d2,

Step 6: Add the added component amount d2 into the culture substrate cavity, and then replace the gas in the gas cavity with a new suitable gas,

Repeat steps 3-6 to obtain data h3-hn and d3-dn, n is an integer greater than three,

When cultivating the strains in the microbial culture system, according to the time h1-hn, when the time reaches a certain hx, the corresponding component amount dx is added to the culture medium cavity of the culture cylinder, and at the same time, the amount of components in the gas chamber of the culture cylinder is The gas is replaced with a new suitable gas, 1≤x≤n, x is an integer.

In the above-mentioned method of the present application, the monitoring of microorganisms adopts the current routine microbial monitoring methods. The specific monitoring process can be obtained without doubt by those skilled in the art using conventional experimental equipment and conventional technical means. Experiments are not described in this application. To repeat, in the actual cultivation process, the inventors found that, in the microbial environment gas, not only the gas discharged during the growth process of the microorganisms, but also the gas volatilized from the culture substrate, and the absorption and use of the culture substrate components by the microorganisms is usually less, In the same period of time, the change of gas composition is greater, and the change caused by microorganisms is more significant than that of culture substrate components. Therefore, in this plan, through the corresponding standards and specifications of different microbial species, the selected microorganism Variation rate threshold and mortality threshold, through each time node, in the culture system, gas replacement and corresponding culture substrate supplementation can be carried out according to the time node, so as to ensure that the microorganisms are in a suitable environment during the whole culture process, and ensure that the microorganisms are cultivated quality.

As a preferred technical solution, before transporting the microbial culture system, estimate the transport time, and calculate the appropriate gas volume and replenishment group required before transporting to the site of use according to the transport time and the number of culture cylinders in the microbial culture system Components, and arrange the required volume of appropriate gas and supplementary components in the tank of the microbial cultivation system.

As a preferred technical scheme, the amount of supplementary components required for the culture system will be grouped according to the actual data h, so that each group of supplementary components corresponds to the supplementary amount of the culture matrix that the entire microbial culture system needs to supplement at different time points. Then divide the amount of each group of components into equal parts according to the number of culture tubes, and pack them into supplementary component packages corresponding to the culture tubes.

As a preferred technical solution, after the culture system is transported to the site of use, the microorganisms in the culture cylinder are mixed together with the culture substrate on the product to be used.

Compared with prior art, the beneficial effect of the present invention:

1. In the culture cylinder of the present application, microorganisms are cultivated in the culture medium in the cylinder, and the gas chamber contains suitable gas. As time goes by, when the gas in the gas chamber is no longer suitable for the growth and development of microorganisms, the gas in the cylinder will The replacement device replaces the gas in the gas chamber with a new suitable gas, so that the risk of death or variation or contamination during the growth and reproduction of microorganisms is reduced, and it also ensures a good development speed of microorganisms and saves cultivation time;

2. The setting of the one-way conduction device makes the one-way one-way conduction device only used for exhausting, so that the sediment during exhausting is prevented from being brought into the cylinder again by the new suitable gas, which will cause adverse effects on microorganisms, and then provide microorganisms quality of cultivation;

3. By setting the U-shaped elbow, it is more conducive to the aggregation of condensed particles in the exhaust gas, reducing the turbulent gas formed by the connecting pipe during the exhaust process, which will bring the particles back to the culture substrate cavity;

4. A through hole is provided on the push rod. This way, after the gas in the gas chamber is discharged, new suitable gas can be replenished into the gas chamber through the through hole of the push rod. In this way, as mentioned above, the gas will be discharged Different channels are used for the gas and the incoming gas, which can avoid the condensed liquid and/or fixed particles in the exhaust gas from entering the culture substrate cavity, thereby further ensuring the culture quality of microorganisms;

On the other hand, the quality of the components of the culture medium is also an important factor affecting the quality of microbial culture. During the growth and reproduction of microorganisms, on the one hand, the absorption of microorganisms consumes part of the components in the culture medium. On the other hand, because The composition analysis of the culture medium, especially the liquid culture medium, after standing still for a long time, some components will settle in the lower part of the liquid. In the case of dormancy or mutation, in the scheme of this application, the corresponding components can be supplemented into the culture substrate cavity through the through hole of the push rod, so as to ensure that the culture substrate components are in a state suitable for the growth and development of microorganisms, and then reliable Ensure the quality of microbial culture;

5. In the microbial cultivation system of the present application, the temperature of the culture medium and the surrounding environment of the microorganisms is also one of the important factors in the cultivation of microorganisms. Whether the temperature is suitable determines the quality of the growth and development of the microorganisms. In the actual cultivation of microorganisms, some microorganisms will Absorbing heat will cause the temperature of the culture substrate to drop, and some microorganisms will release heat, causing the temperature of the culture substrate to rise. These changes in temperature often inhibit the further growth and development of microorganisms, and may even cause mutation and death in severe cases situation; therefore, in the scheme of this application, by setting the temperature control chamber, the temperature in the temperature control chamber can be controlled and adjusted, and the temperature in the chamber can be kept stable, so that the temperature in the culture cylinder, especially the culture substrate chamber The temperature is at a temperature suitable for the growth, development and reproduction of microorganisms, thereby ensuring the good cultivation quality of microorganisms;

6. Compared with the traditional method, the microbial cultivation method of the present application adopts the microbial cultivation system of the present application in the transportation link, so it directly avoids the preservation steps and recovery steps of the microbial strains in the traditional method, so, First of all, the cost of preservation and recovery is avoided, and more importantly, the risk of microbial death and mutation in the traditional preservation and recovery process is avoided. Moreover, with the method of this application, after the microbial strain is cultivated, it directly enters the The cultivation of microorganisms in the culture system reduces the risk of microbial variation and improves the purity of microorganisms. Moreover, when transported to the use site for use, the microorganisms have excellent activity, and they have also grown to a certain number, greatly shortening the production time. The time period of on-site use is shortened, so that the use cost is also greatly reduced. The microbial cultivation method of the present application is especially suitable for the situation that the use site needs to be used immediately or in a short period of time;

7. According to the corresponding standards and norms of different microbial varieties, select the microbial variation rate threshold and mortality threshold, and through each time node, in the culture system, it is enough to perform gas replacement and corresponding culture medium supplement according to the time node to ensure During the whole cultivation process, microorganisms are in a suitable environment to ensure the quality of microbial cultivation.

Description of drawings

Fig. 1 is the structural representation of cylinder body in the specific embodiment;

Fig. 2 is a structural schematic diagram of the cooperation between the temperature control cabin and the cylinder and the box in the specific embodiment,

Marked in the figure: 1-Cylinder body, 2-Cover body, 3-Cultivation substrate chamber, 4-Gas chamber, 5-Connecting pipe, 6-One-way conducting device, 7-U-shaped elbow, 8-Push plate, 9 -Push rod, 10-Through hole, 11-Blocking cover, 12-Guide, 13-Nozzle, 14-Observation window, 15-Temperature control cabin, 16-Temperature control chamber, 17-Sealing gasket, 18-Box Body, 19-gas chamber, 20-exhaust gas chamber.

Implementation

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are some, not all, embodiments of the present invention.

Therefore, the following detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely represents some embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

It should be noted that, in the case of no conflict, the embodiments of the present invention and the features and technical solutions in the embodiments can be combined with each other.

It should be noted that like numerals and letters denote similar items in the following figures, therefore, once an item is defined in one figure, it does not require further definition and explanation in subsequent figures.

In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", etc. is based on the orientation or positional relationship shown in the drawings, or the conventionally placed position when the product of the invention is used. Orientation or positional relationship, or the orientation or positional relationship commonly understood by those skilled in the art, such terms are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the referred device or element must have a specific orientation, Constructed and operative in a particular orientation and therefore are not to be construed as limitations of the invention. In addition, the terms "first", "second", etc. are only used for distinguishing descriptions, and should not be construed as indicating or implying relative importance.

Embodiment 1, as shown in Figures 1 and 2:

A cylinder for cultivating microorganisms, comprising a cylinder 1 with a closed lower end and an open upper end and a cover 2 matched with the open end of the upper end of the cylinder 1, and the inner space of the cylinder 1 is divided from bottom to top into Culture substrate chamber 3 and gas chamber 4, described culture substrate chamber 3 is used for containing the culture medium suitable for microbial growth, and described gas chamber 4 is used for containing the suitable gas suitable for microbial growth, also is provided with in described barrel 1 A gas replacement device, the gas replacement device is used to replace the gas in the gas chamber 4 with a new suitable gas.

In the scheme of this application, the culture medium suitable for microorganism cultivation is the current conventional culture medium, which can be a liquid medium, or a solid medium or a colloidal medium. For different types of microorganisms, the formula of the medium Different from the morphology, for different types of microorganisms, the gas formula suitable for the reproduction and growth of microorganisms may also be different. The formula, shape of the medium, and the gas suitable for microorganisms are those skilled in the art based on the conventional techniques in the field mastered by those skilled in the art. Different types of microorganisms can be selected or produced to obtain suitable medium and gas suitable for such microorganisms, so details will not be repeated in this content.

In the current cultivation of microorganisms, the periodic gas composition is the key factor affecting the growth and development of microorganisms. When the surrounding gas composition changes greatly, the reproduction, growth and development of microorganisms will be affected, and even cause the death or mutation of microorganisms in severe cases. , or there may be problems such as pollution, which is also the key purpose of the current strain preservation. In actual work, the inventor found that during the growth and reproduction of microorganisms, whether they are aerobic microorganisms or anaerobic microorganisms, most of them will It affects the air around it. The growth and development of some microorganisms will absorb some components in the surrounding gas, and some microorganisms will release some gases. Some microorganisms absorb certain gases and emit some gases at the same time. Therefore, as As time goes by, the growth of microorganisms progresses, and the surrounding gas will also change accordingly, which leads to the risk of death, mutation and contamination due to changes in gas composition during the growth and development of microorganisms. Based on the above, in the scheme of the present application, the microorganisms are cultured in the culture medium in the cylinder body 1, and the gas chamber 4 contains suitable gas. As time goes by, when the gas in the gas chamber 4 is no longer suitable for the growth and reproduction of microorganisms , the gas in the gas chamber 4 is replaced by a new suitable gas through the gas replacement device in the cylinder 1, so that the risk of death or variation or contamination during the growth and reproduction of microorganisms is reduced, and the good health of the microorganisms is also ensured. development speed and save training time.

Embodiment 2, as shown in Figures 1 and 2:

A gas replacement device for the culture cylinder of the above embodiment, the gas replacement device includes a replacement component and a gas discharge component, the gas discharge component is used to communicate the gas chamber 4 with the external space of the cylinder body 1, the The replacement component is used to push the gas in the gas cavity 4, so that the gas in the gas cavity 4 is discharged out of the barrel 1 by the gas discharge component.

As a preferred embodiment, the gas exhaust assembly includes a communication pipe 5, one end of which communicates with the gas chamber 4, and the other end communicates with the external space of the cylinder 1 through the cylinder 1 .

As a preferred embodiment, a cut-off valve is also provided on the communicating pipe 5 . The communication and blocking states of the communication pipe 5 are controlled by the opening and closing of the cut-off valve. By setting the shut-off valve, the shut-off valve is in a normally closed state. When the gas in the gas chamber 4 needs to be replaced, the shut-off valve is opened, and the gas in the gas chamber 4 is pushed through the replacement component, so that the gas in the gas chamber 4 is discharged from the connecting pipe 5. Then fill the gas chamber 4 with new suitable gas through the connecting pipe 5, so as to realize the replacement of the gas.

As another preferred embodiment, a one-way conducting device 6 is also provided on the communication pipe 5, so that the communication pipe 5 is unidirectional in the direction from the inside of the cylinder body 1 to the outside of the cylinder body 1. Pass. The air outside the cylinder body 1 cannot enter the gas cavity 4 through the sealing assembly. In actual use, the inventors found that since the gas in the gas chamber 4 is usually relatively humid, after the connecting pipe 5 is exhausted many times, it is very likely that the liquid droplets will gather in the connecting pipe 5, and even more After a long time, it dries into solid or dust particles. If the connecting pipe 5 is used to blow new suitable gas into the gas chamber 4, these particles may be sent back to the gas chamber 4 thereupon and fall onto the culture medium. Abnormal substances with extremely high concentrations are formed on these particles and the surrounding culture medium, which can easily cause local microorganisms to mutate and die, thereby affecting the cultivation effect of the entire microorganisms. Therefore, in the scheme of this application, the one-way conduction device 6 It is set so that the one-way and one-way conduction device 6 is only used for exhausting, so that the sediment during exhausting is prevented from being brought into the cylinder body 1 again by the newly introduced suitable gas to cause adverse effects on microorganisms, and then the cultivation quality of microorganisms is improved.

As a preferred embodiment, the one-way conduction device 6 is arranged on one end of the communication pipe 5 close to the gas cavity 4 . In this way, it is further ensured that the pollutants in the connecting pipe 5 do not enter the cylinder body 1 , and at the same time, it is also ensured that the gas in the gas chamber 4 is discharged as much as possible, so as to prevent the residual gas in the pipeline from returning to the gas chamber 4 .

As a preferred embodiment, the one-way passage device 6 is a one-way valve.

As a preferred embodiment, the communication pipe 5 is arranged vertically along the inner wall of the gas chamber 4, the lower end of the communication pipe 5 is in clearance fit with the upper edge of the culture substrate chamber 3, and the communication pipe 5 The upper end passes through the upper end part of the cylinder body 1 to form an exhaust nozzle. The connecting pipe 5 is vertically arranged on the side wall of the gas chamber 4, and the exhaust nozzle is located at the upper part of the cylinder body 1, so that the exhaust nozzle has a larger operating space, avoiding that when the exhaust nozzle is at a low position, in order to reserve the operation space between the plurality of cylinders 1; moreover, the connecting pipe 5 is arranged on the inner wall of the gas chamber 4, so that the temperature of the connecting pipe 5 is consistent with the temperature in the gas chamber 4, and the condensation when the gas enters the connecting pipe 5 is reduced. The possibility of forming liquid droplets, thereby reducing the risk of pollution of the exhaust gas to the communication pipe 5.

As another preferred embodiment, the communication pipe 5 includes a piercing section and a vertical section, the vertical section is vertically arranged along the outer wall of the cylinder 1, and one end of the piercing section is located at the cylinder body 1, which fits with the upper edge of the culture substrate cavity 3, and the other end passes through the cylinder body 1 and connects with the lower end of the vertical section. In this solution, the vertical section of the connecting pipe 5 is arranged outside the cylinder body 1, which can effectively avoid occupying the internal space of the cylinder body 1, and can also ensure the smoothness of the inside of the cylinder body 1, reducing the existing gaps in the cylinder body 1 Etc., reduce the problem that the culture substrate gathers in the gap and cause high-concentration culture substrate to appear in this part, and then avoid the adverse effects caused by these high-concentration culture substrates falling into the culture substrate below.

As a further preferred embodiment, the passage section is inclined downward from the inside of the cylinder 1 to the outside of the cylinder 1 . The passage section is inclined downward. As mentioned above, when the exhaust gas is condensed into droplet particles in the communication pipe 5, these particles can be prevented from falling into the culture substrate chamber 3, thereby ensuring good culture quality of microorganisms.

As a further preferred embodiment, a downwardly bent U-shaped elbow 7 is connected between the passing-through section and the vertical section. By setting the U-shaped elbow 7 , it is more conducive to the aggregation of condensed particles in the exhaust gas, and reduces the turbulent gas formed by the connecting pipe 5 to bring the particles back into the culture substrate chamber 3 during the exhaust process.

As a further preferred embodiment, a drain valve is also provided at the bottom of the U-shaped elbow 7 . When needed, the drain valve can be opened to discharge the liquid and/or solid particles in the U-shaped elbow 7 .

As a preferred embodiment, the replacement assembly includes a push plate and a push rod 9 arranged on the upper side of the push plate, the push rod 9 is vertically arranged, and passes through the cover body 2, and the push rod 9 The rod 9 is in a sliding and sealing fit with the cover body 2 , and the push plate is in a sliding and sealing fit with the side wall of the gas chamber 4 . In this solution, the push rod 9 can be driven by manpower or set to be driven by a motor. By setting the push plate and the push rod 9, in the initial position, the push plate is located above the gas chamber 4. The rod 9 pushes the push plate, so that the push plate moves down, pushes the gas in the gas chamber 4 into the gas discharge assembly, and then realizes the discharge of the gas. In this way, new gas can be replenished into the gas chamber 4 from the connecting pipe 5 .

As a preferred embodiment, when the communication pipe 5 is located in the cylinder body 1, a notch is provided on the push plate corresponding to the communication pipe 5, and the gap between the notch of the push plate and the communication pipe 5 There is a sliding seal fit between them. The gap provided on the push plate to cooperate with the connecting pipe 5 also makes the part of the connecting pipe 5 located in the cylinder 1 a guiding structure, avoiding the rotation of the push plate in the circumferential direction, reducing the wear on the edge of the push plate, and ensuring that the push plate The sealing reliability with the side wall of the gas chamber 4.

As a preferred embodiment, the push rod 9 is provided with a through hole 10 that runs through the push rod 9 from top to bottom, and the through hole 10 runs through the push plate, and the upper end of the push rod 9 is detachably set There is a closure cap 11 for closing said through hole 10 . A through hole 10 is provided on the push rod 9 in such a way that after the gas in the gas chamber 4 is discharged, new suitable gas can be replenished into the gas chamber 4 through the through hole 10 of the push rod 9. In this way, as mentioned above Yes, different channels are used for the exhaust gas and the inlet gas, which can prevent the condensed liquid and/or fixed particles in the exhaust gas from entering the culture substrate cavity 3, thereby further ensuring the culture quality of microorganisms;

On the other hand, the quality of the components of the culture medium is also an important factor affecting the quality of microbial culture. During the growth and reproduction of microorganisms, on the one hand, the absorption of microorganisms consumes part of the components in the culture medium. On the other hand, because The composition analysis of the culture medium, especially the liquid culture medium, after standing still for a long time, some components will settle in the lower part of the liquid. In the case of dormancy or variation, in the scheme of the present application, corresponding components can be supplemented into the culture substrate cavity 3 through the through hole 10 of the push rod 9, so as to ensure that the culture substrate components are in a state suitable for the growth and development of microorganisms, Thus, the quality of microbial culture can be reliably ensured.

As a preferred embodiment, the lower side of the push plate is in the shape of a cone with the middle part protruding upward, and the through hole 10 of the push rod 9 is located at the top of the lower tapered surface of the push plate. The lower side of the push plate is set as a conical shape with the middle part protruding upwards. When the liquid culture medium enters the through hole 10 and flows down to the underside of the push plate, it can flow radially downward along the tapered surface, and then Improve the uniformity of the dispersion of the added culture medium.

As a further preferred embodiment, a plurality of flow guide grooves 12 along the direction of the generatrix are provided on the lower side of the push plate, and each flow guide groove 12 is evenly distributed around the circumference. Through the setting of the guide groove 12, the uniformity of dispersion of the culture medium in the circumferential direction is further improved.

As a further preferred embodiment, a spray head 13 connected to the through hole 10 is also provided on the lower side of the push plate corresponding to the through hole 10, and the spray head 13 is used to spray the liquid culture substrate into a mist. When the culture substrate is liquid, by setting the nozzle 13, the supplemented culture medium is sprayed into a mist, and these mist liquid particles gradually settle in the culture substrate cavity 3 to ensure the uniformity of dispersion, and further ensure the cultivation of microorganisms quality.

As a preferred embodiment, an observation window 14 is also provided on the side wall of the cylinder 1, and the observation window 14 is arranged on the side wall of the cylinder 1 corresponding to the gas cavity 4, and the edge of the observation window 14 Fitting sealingly with the edge of the barrel 1, the observation window 14 is made of transparent material. There is an opening on the cylinder body 1, and an observation window is provided, which is convenient for visual observation of the microorganisms and the conditions of each component in the cylinder body 1, and is convenient for the operator to operate.

As a preferred embodiment, a barometer for monitoring the air pressure in the gas chamber 4 is also provided on the cylinder body 1 . The setting of the barometer facilitates the control of the air pressure in the gas chamber 4 and at the same time facilitates the monitoring of the amount of added gas.

Embodiment 3, as shown in Figures 1 and 2:

A microbial culture system, including the above-mentioned microbial culture tube, also includes a temperature control cabin 15, the temperature control cabin 15 has a temperature control chamber 16 capable of maintaining a stable temperature in the cavity, all or the lower part of the culture tube As for the inside of the temperature control chamber 16 . In microbial culture, the temperature of the medium and the surrounding environment of the microorganism is also one of the important factors. Whether the temperature is suitable determines the quality of the growth and development of the microorganism. In actual microbial cultivation, some microorganisms will absorb heat, resulting in the temperature of the culture substrate Some microorganisms will release heat, leading to an increase in the temperature of the culture substrate. These changes in temperature often inhibit the further growth and development of microorganisms, and in severe cases, mutations and death may even occur.

Therefore, in the scheme of the present application, by setting the temperature control chamber 15, the temperature in the temperature control chamber 16 can be controlled and adjusted, and the temperature in the chamber can be kept stable, so that the temperature in the culture cylinder, especially the culture substrate chamber 3 are all at a temperature suitable for the growth, development and reproduction of microorganisms, thereby ensuring the good cultivation quality of microorganisms.

As a preferred embodiment, the temperature control cabin 15 has a closed cavity filled with heat transfer material, and the temperature control cabin 15 is also provided with a heating device for heating the heat transfer material. And/or the temperature regulating device of refrigeration, on the upper side plate of described temperature control cabin 15, also be provided with the sunken cavity that is sunken in described cabin, described culture cylinder is placed in described sunken cavity, and described sunken cavity The side wall matches the outer wall of the culture cylinder. The recessed cavity is the temperature control cavity 16. In this way, by setting the temperature adjustment device to a suitable temperature, by setting the heat transfer material in the closed cavity, the heat transfer material can be water, oil, etc. The substance that evenly heats the concave cavity can also be air, so that the temperature in the concave cavity can be controlled, and then the temperature in the microbial cultivation cylinder can be controlled.

As a preferred embodiment, a flexible gasket 17 is also provided on the upper edge of the concave cavity, and the gasket 17 is used to seal the gap between the outer wall of the culture cylinder and the concave cavity. The arrangement of the sealing gasket 17 is to ensure good heat transfer between the concave cavity and the cylinder body 1 on the one hand, and to improve the stability of the cylinder body 1 on the other hand.

As a preferred embodiment, the gasket 17 is a rubber gasket 17 .

As a preferred embodiment, the temperature control cabin 15 is provided with a plurality of the recessed cavities, and each of the recessed cavities is provided with a culture tube. It can greatly improve the culture efficiency, and when cultivating the same number of microorganisms, it can greatly reduce the number of microorganisms in a single cylinder 1, while improving the culture quality, it also greatly reduces the risk of cross-infection between microorganisms, and also utilizes the Control of microbial mutations.

As a preferred embodiment, there is a distance between adjacent concave cavities, so that after the culture tubes are arranged in the concave cavities, a passage for people to pass is formed between the culture tubes. It is convenient for staff to monitor and operate each culture cylinder.

As a preferred embodiment, the microbial cultivation system also includes a box body 18, the temperature control cabin 15 and the culture cylinder are arranged in the box body 18, and the temperature control cabin 15 and the box body 18 are detachable Connection. By setting the box body 18, the integrity of the culture system is improved, the pollution of the culture cylinder is reduced, and the transportation is also convenient.

As a preferred embodiment, the box body 18 is a container.

As a preferred embodiment, a gas chamber 19 is also provided in the box body 18, and the gas chamber 19 is used to store a gas suitable for the cultured microorganisms. The aeration pipeline that the through hole 10 of culture tube push rod 9 is matched. The gas chamber 19 has a closed cavity in which new unused gas is stored. The air filling pipe is provided with an air filling nozzle that is also compatible with the push through hole 10, and the air filling nozzle is in a detachable sealing fit with the push rod 9. After the air in the gas cavity 4 in the cultivation cylinder is discharged, the through hole 10 of the push rod 9 is matched by the aeration pipeline to supplement new suitable gas suitable for the growth and development of microorganisms in the gas cavity 4 .

As a preferred embodiment, the gas chamber 19 is arranged on the top of the box body 18 .

As a preferred embodiment, the gas chamber 19 includes a lower side plate, and the edge of the lower side plate and the side wall of the box body 18 are in a sliding and sealing fit. The gas storage space of the gas chamber 19 is formed between the 18 top plates, the gas filling pipe is arranged on the lower side plate, and communicates with the gas storage space of the gas chamber 19, and in the box body 18 A drive device is provided to drive the lower side plate to move vertically. In this application, the driving device is a driving motor, which drives the lower side plate to move, and presses the gas in the gas chamber 19 into the gas chamber 4 of the culture cylinder to realize the filling of suitable gas.

As a preferred embodiment, an exhaust gas chamber 20 is also provided in the box body 18, and the exhaust gas chamber 20 communicates with the communication pipes 5 of each of the culture cylinders. Corresponding to the cultivation of certain microorganisms, the suitable gas in the gas chamber 4 may cause serious pollution or poisonous and harmful. For such toxic and harmful gases, in the solution of this application, the waste gas chamber 20 is used for collection, and then concentrated for harmless treatment deal with.

Embodiment 4, as shown in Figures 1 and 2:

A method for cultivating microorganisms using the above-mentioned microorganism cultivation system. After obtaining the microorganism strains, the strains are cultivated in the microorganism cultivation system, and then the microorganism cultivation system cultivated with the microorganism strains is transported to the use site, and then the microorganism strains are cultivated in the cultivation system. Microorganisms are taken out for on-site use.

The microbial cultivation method of the present application, compared with the traditional method, because the microbial cultivation system of the present application is used in the transportation link, it directly avoids the microbial strain preservation step and the recovery step in the traditional method, so, first of all, The cost of preservation and recovery is eliminated, and more importantly, the risk of microbial death and mutation in the traditional preservation and recovery process is avoided. Moreover, with the method of this application, after the microbial strain is cultivated, it directly enters the culture system The cultivation of microorganisms in the environment reduces the risk of microbial variation and improves the purity of microorganisms. Moreover, when transported to the use site for use, the microorganisms have excellent activity, and they have also grown to a certain number, which greatly shortens the time spent on site. The time period of use is like this, and the cost of use is also greatly reduced. The microorganism cultivation method of the present application is especially suitable for the situation where the use site needs to be used immediately or in a short period of time.

As a preferred embodiment, after the microbial strains are obtained, before the strains are cultivated in the microbial culture system, the time node experiment of gas replacement and culture substrate replenishment in the microbial culture system is carried out,

The gas replacement and culture substrate filling time node experiment of the microorganism culture system is as follows: the temperature control cabin 15 is set to a temperature suitable for the growth, development and reproduction of microorganisms, and according to the loading amount of the culture substrate in the culture cylinder and the setting of microorganism strains in the actual transportation process. Put the culture substrate and strains into the culture substrate chamber 3 of the culture cylinder, then fill the gas chamber 4 of the culture cylinder with suitable gas, start timing, record the time reading h0, according to the actual microbial species and the requirements of the field of use , select the microbial variation rate threshold and the mortality threshold, and then perform the following steps:

Step 1: Monitor the microorganisms in the culture medium, when either the variation rate or the death rate of the microorganisms in the culture cylinder reaches 90-95% of the threshold value, record the time reading h1,

Step 2: When detecting h1, the composition of the culture substrate and the ratio of each ingredient are compared, and the ratio of the ingredients is compared with the ratio of the original ingredients of the culture substrate, and the amount of components that need to be supplemented d1 is calculated.

Step 3: Add the added component amount d1 into the culture substrate cavity 3, and then replace the gas in the gas cavity 4 with a new suitable gas,

Step 4: Continue to monitor the variation rate and mortality of microorganisms. When any of the variation rate and mortality of microorganisms in the culture cylinder reaches 90-95% of the threshold, record the time reading h2,

Step 5: When detecting h2, the composition of the culture substrate and the ratio of each ingredient are compared, and the ratio of the ingredients is compared with the ratio of the original composition of the culture substrate to calculate the amount of components that need to be supplemented d2,

Step 6: Add the added component amount d2 into the culture substrate cavity 3, and then replace the gas in the gas cavity 4 with a new suitable gas,

Repeat steps 3-6 to obtain data h3-hn and d3-dn, n is an integer greater than three,

When cultivating the strains in the microbial culture system, according to the time h1-hn, when the time reaches a certain hx, the corresponding component amount dx is added to the culture medium chamber 3 of the culture cylinder, and at the same time, the gas chamber 4 of the culture cylinder The gas in is replaced with a new suitable gas, 1≤x≤n, x is an integer.

As a preferred embodiment, before the transportation of the microbial culture system, the transportation time is estimated, and according to the transportation time and the number of culture cylinders in the microbial culture system, the appropriate gas volume and replenishment group required before transportation to the use site are calculated Components, and the appropriate gas of the required volume and the amount of supplementary components are arranged in the casing 18 of the microorganism cultivation system. In this scheme, by estimating the culture time of microorganisms in the microbial culture system, and then combining the test data with the culture time, the appropriate gas volume and the amount of culture medium components are estimated, because these are those skilled in the art through conventional knowledge. Therefore, no further elaboration will be made in this application.

As a preferred embodiment, the amount of supplementary components required for the culture system will be grouped according to the actual data h, so that the amount of supplementary components in each group corresponds to the supplementary amount of the culture matrix that the entire microbial culture system needs to supplement at different time points. Then divide the amount of each group of components into equal parts according to the number of culture tubes, and pack them into supplementary component packages corresponding to the culture tubes. In actual use, at different time nodes, select the corresponding grouping, and then add the culture matrix replenishment component of one of the component packages into the culture matrix cavity 3 of the culture cylinder. In terms of operation process, and Good quantification ensures the accuracy of added ingredients.

As a preferred embodiment, after the culture system is transported to the site of use, the microorganisms in the culture cylinder are mixed with the culture substrate on the product for use. This method makes the culture substrate more fully utilized, especially can form a good microbial environment buffer, and avoid too much adverse effects on the growth and reproduction of microorganisms caused by excessive environmental gaps. This method is especially suitable for microorganisms that are lactic acid bacteria. Fermentation work on poultry feed.

The above embodiments are only used to illustrate the present invention and are not intended to limit the technical solutions described in the present invention. Although the specification has described the present invention in detail with reference to the above-mentioned embodiments, the present invention is not limited to the above-mentioned specific implementation methods, so Any modifications or equivalent replacements to the present invention; and all technical solutions and improvements that do not depart from the spirit and scope of the invention are covered by the scope of the claims of the present invention.

Claims (3)

1. A method of culturing a microorganism, characterized by: the culture cylinder is adopted to culture the microorganism,

the culture cylinder comprises a cylinder body with an upper end being opened and a lower end being closed, and a cover body matched with the upper end opening end of the cylinder body, wherein the inner space of the cylinder body is divided into a culture medium cavity and a gas cavity from bottom to top, the culture medium cavity is used for containing culture medium suitable for microorganism growth, the gas cavity is used for containing suitable gas suitable for microorganism growth, and a gas replacement device is also arranged in the cylinder body and is used for replacing the gas in the gas cavity with new suitable gas;

the gas displacement device comprises a displacement assembly and a gas discharge assembly, wherein the gas discharge assembly is used for communicating the gas cavity with the outer space of the cylinder, and the displacement assembly is used for pushing gas in the gas cavity so that the gas in the gas cavity is discharged out of the cylinder through the gas discharge assembly;

The gas discharge assembly comprises a communicating pipe, one end of the communicating pipe is communicated with the gas cavity, and the other end of the communicating pipe penetrates through the cylinder body to be communicated with the external space of the cylinder body;

the communicating pipe is also provided with a one-way conduction device, so that the communicating pipe is conducted unidirectionally in the direction from the inside of the cylinder to the outside of the cylinder;

the one-way conduction device is arranged at one end, close to the gas cavity, of the communicating pipe;

the communicating pipe comprises a penetrating section and a vertical section, the vertical section is vertically arranged along the outer wall of the cylinder, one end of the penetrating section is positioned in the cylinder and is in clearance fit with the upper edge of the culture medium cavity, the other end of the penetrating section penetrates out of the cylinder and is connected with the lower end of the vertical section, and the penetrating section is inclined downwards in the direction from the inside of the cylinder to the outside of the cylinder;

a U-shaped bent pipe which is bent downwards is connected between the penetrating section and the vertical section;

a drain valve is further arranged at the bottom of the U-shaped bent pipe, and can be opened to discharge liquid and/or solid particles in the bent pipe when needed;

the replacement assembly comprises a push plate and a push rod arranged on the upper side of the push plate, the push rod is vertically arranged and penetrates through the cover body, sliding sealing fit is formed between the push rod and the cover body, and the push plate is in sliding sealing fit with the side wall of the gas cavity;

When the communicating pipe is positioned in the cylinder body, a notch is arranged on the push plate corresponding to the communicating pipe, and the notch of the push plate is in sliding sealing fit with the communicating pipe;

a through hole penetrating through the push rod from top to bottom is formed in the push rod, the through hole penetrates through the push plate, and a plugging cover for closing the through hole is detachably arranged at the upper end of the push rod;

the lower side of the push plate is in a conical shape with an upward convex middle part, a through hole of the push rod is positioned at the top of the conical surface at the lower side of the push plate, a plurality of diversion trenches along the bus direction are arranged at the lower side of the push plate, the circumference of each diversion trench is uniformly distributed, a spray head connected with the through hole is also arranged at the lower side of the push plate corresponding to the through hole, and the spray head is used for spraying the liquid culture medium into mist;

the microorganism culture method further comprises a temperature control cabin, wherein a temperature control cavity capable of keeping the temperature in the cavity stable is arranged in the temperature control cabin, all or the lower part of the culture cylinder is arranged in the temperature control cavity, the temperature control cabin is provided with a closed cavity filled with a heat transfer substance, the temperature control cabin is also provided with a temperature regulating device for heating and/or refrigerating the heat transfer substance, the upper side plate of the temperature control cabin is also provided with a concave cavity recessed into the cabin body, the culture cylinder is arranged in the concave cavity, the side wall of the concave cavity is matched with the outer wall of the culture cylinder, the concave cavity is the temperature control cavity,

The upper edge of the concave cavity is also provided with a flexible sealing gasket which is used for sealing the gap between the outer wall of the culture cylinder and the concave cavity,

after the microbial strain is obtained, culturing the strain in a microbial culture system, transporting the microbial culture system cultured with the microbial strain to a use site, and taking out the microorganism in the culture system for use on site;

after the microbial strains are obtained, the gas replacement and culture medium feeding time node experiment of the microbial culture system is performed before the strains are cultured in the microbial culture system.

2. The method of claim 1, wherein: and a stop valve is also arranged on the communicating pipe.

3. The method for culturing microorganisms according to claim 1 or 2, wherein: the communicating pipe is vertically arranged along the inner wall of the gas cavity, the lower end of the communicating pipe is in clearance fit with the upper edge of the culture substrate cavity, and the upper end of the communicating pipe penetrates through the upper end part of the cylinder body to form an exhaust nozzle.