CN110865416A - Method for measuring plant biochemical indication under radioactive stress - Google Patents

Abstract

A method for measuring plant biochemical indications under radioactive stress comprises culturing different plants in a laboratory environment, observing and measuring the physiological structure change of the plants under the uranium stress and the distribution rule of uranium at different parts of leaves from a biochemical perspective, and simultaneously detecting the enrichment content of uranium elements, carotenoid, soluble protein, malonaldehyde and chlorophyll content at the root, stem and leaf parts and the influence of uranium on the activities of superoxide dismutase, catalase and peroxidase, analyzing the influence of uranium on the plant biochemical indications in an ore exploration working area by the method, thereby obtaining uranium exploration information in the area and facilitating the further exploration and development of uranium resources, the method provides a new direction for uranium exploration by utilizing a biological biochemical indication research method, and has the characteristics of convenient material acquisition, low cost, simple and convenient processing steps, environmental friendliness, no destruction and the like, the obtained data is new uranium ore exploration and ore finding data, the data indicative is obvious, and the ore finding efficiency is improved.

Description

A method for measuring plant biochemical indicators under radioactive stress

technical field

The invention relates to a geological exploration measurement method, in particular to a plant biochemical indicator measurement method under radioactive stress for uranium exploration.

Background technique

Uranium is a radioactive metal element with active chemical properties, wide distribution and high crustal abundance (2.7×10 ^-6 g/g). It is the raw material for the manufacture of nuclear weapons and nuclear fuel, and the nuclear energy produced by it has remarkable characteristics such as cleanliness, safety and environmental protection. The vigorous development of my country's nuclear power industry has created a huge demand for uranium resources, while southern uranium mining companies generally face resource depletion and high refining costs, while northern uranium mining companies are mostly faced with in-situ leaching of sandstone uranium mines, and some sandstone-type uranium mines have just started to develop in-situ leaching. Due to unsatisfactory conditions and other problems, it is particularly urgent to propose new uranium exploration and measurement methods to help find new uranium resources.

At present, researchers have found that the technology of uranium exploration from the perspective of plants is feasible by using the stress effect of uranium on plants. The physiological and biochemical indicators of different standard plants cultured in different concentrations of uranium culture solution were measured in the laboratory, and compared with the physiological and biochemical indicators of the plants collected in the exploration area during the same period, it is easy to obtain the information of uranium mineralization in the exploration area. In terms of traditional data collection such as geology, geochemistry and geophysics, the data collection efficiency is high and the cost is low, which can lay a physiological and biochemical foundation for further remote sensing hyperspectral prospecting work.

However, there is no complete, mature and feasible solution for the screening, cultivation and biochemical index measurement of standard plants by this technology, and tracer information such as vegetation physiological and biochemical characteristics is not used enough in uranium prospecting work.

SUMMARY OF THE INVENTION

The purpose of the present invention is to solve the problems raised in the background technology, and to provide a plant biochemical indicator measurement that can reduce exploration cost, reduce exploration difficulty, is easy to operate, has a significant tracer effect, and is used for uranium exploration under radioactive stress method.

The technical solution of the present invention is, a method for measuring plant biochemical indicators under radioactive stress, which is characterized by comprising the following steps:

1. Material selection: Select plants with uniform shape and size and the same growth period for hydroponics, cultivate with uranium-free nutrient solution for three days, and then select plants with the same growth potential to continue cultivation;

2. Preparation of uranium-containing base solution: Take 1.1790g of standard U ₃ O ₈ in a beaker, add 10ml of concentrated hydrochloric acid and 3ml of hydrogen peroxide, then drop 0.1ml of nitric acid into the beaker, place it on a heating electric furnace and heat it on a small fire, stir to dissolve to a yellow translucent liquid, and then dilute to 1L with distilled water to prepare a uranium base solution with a mass concentration of 1g/L;

3. Hydroponics: use distilled water to make the basic solution into seven concentrations of uranium containing 1 mg/L, 2 mg/L, 10 mg/L, 50 mg/L, 100 mg/L and 200 mg/L. For the base culture medium, set 3 to 5 identical culture samples for each concentration for hydroponics, and then supplement the modified Hoagland's nutrient solution without uranium according to the consumption of the base culture medium;

4. Sampling and testing: Take fresh plant leaves cultured at different concentrations every 5 to 7 days, wash and remove thick veins, smash them, and measure and record parameters. The measurement parameters include chlorophyll a, chlorophyll b, carotenoids, and soluble protein. , malondialdehyde (MDA) and uranium enrichment content, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities.

A further scheme is: in the hydroponics step, the illumination time in the culture environment is 24 hours a day, which is beneficial to the growth of plants, shortens the experiment time, improves the experiment efficiency, and facilitates quantitative illumination.

A further scheme is: in the hydroponic step, the temperature in the culture environment is controlled at 25°C to 30°C, and the humidity is controlled at 60% to 80%, which is beneficial to the growth of plants, and is beneficial to improve measurement efficiency and control experiment accuracy.

A further scheme is: in the sampling and detection step, plants within the range of the 7th day to the 35th day in the hydroponics process are selected for detection, and the measurement results of each interval within this time range are obviously different and the effect is the most ideal.

The further scheme is: the test plants selected in the described material selection process are Manjianghong, water hyacinth, mangosteen, broccoli, Commelina officinalis, Commelina sinensis and Dieffenbachia, and these plants are common in uranium mining areas and nearby water bodies, absorb Uranium has strong ability, easy hydroponics and measurement data obtained from experiments can more accurately reflect the changes of biochemical indicators of plants under uranium radioactive stress.

The present invention adopts the above technical scheme, by cultivating different plants in a laboratory environment, from the perspective of biochemistry, observing and measuring the changes in the physiological structure of plants under uranium stress and the distribution law of uranium in different parts of leaves, and at the same time detecting the effect of uranium elements in roots, stems and leaves. The enrichment content, carotenoid, soluble protein, malondialdehyde (MDA) and chlorophyll a, b content and uranium to superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) ) activity, this method analyzes the influence of uranium on plant biochemical indicators in the prospecting work area, so as to obtain uranium prospecting information in the area, which is conducive to further exploration and development of uranium resources. The indication research method provides a new direction for uranium exploration. It has the characteristics of convenient material acquisition, low cost, simple processing steps, environmental friendliness and non-destructiveness. The obtained data is a new kind of uranium exploration and prospecting data. It has obvious properties and improves the ore prospecting efficiency.

Detailed ways

Example 1

A method for measuring plant biochemical indicators under radioactive stress, comprising the following steps:

1. Material selection: Select plants with uniform shape and size and the same growth period for hydroponics, cultivate with uranium-free nutrient solution for three days, and then select plants with the same growth potential to continue cultivation;

2. Preparation of uranium-containing base solution: Take 1.1790g of standard U ₃ O ₈ in a beaker, add 10ml of concentrated hydrochloric acid and 3ml of hydrogen peroxide, then drop 0.1ml of nitric acid into the beaker, place it on a heating electric furnace and heat it on a small fire, stir to dissolve to a yellow translucent liquid, and then dilute to 1L with distilled water to prepare a uranium base solution with a mass concentration of 1g/L;

3. Hydroponics: Use distilled water to make the base solution into seven concentrations of uranium with concentrations of 1 mg/L, 2 mg/L, 10 mg/L, 50 mg/L, 100 mg/L, and 200 mg/L. For the basal medium, set 3 identical culture samples for each concentration for hydroponics, and then supplement the modified Hoagland's nutrient solution without uranium according to the consumption of the basal medium;

4. Sampling and testing: Take fresh plant leaves cultured at different concentrations every 7 days, wash and remove thick veins and then smash them to measure and record parameters. The measurement parameters include chlorophyll a, chlorophyll b, carotenoids, soluble protein, C Dialdehyde (MDA) and uranium enrichment content, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities.

In the hydroponic step, the illumination time in the culture environment is 24 hours a day.

In the hydroponic step, the temperature in the culture environment is controlled at 27°C, and the humidity is controlled at 70%.

In the sampling and detection step, plants within the range of the 7th day to the 35th day in the hydroponics process are selected for detection.

The test plants selected in the described material selection process are Manjianghong, water hyacinth, mangosteen, broccoli, Commelina, purple Commelina and Evergreen.

Claims (8)

1. A method for measuring the biochemical indicators of plants under radioactive stress, which is characterized by comprising the following steps:

(1) selecting materials: selecting plants with uniform shapes and sizes and the same growth period for water culture, culturing for three days by using a nutrient solution without uranium, and selecting the plants with the same growth vigor for continuous culture;

(2) preparing a uranium-containing base liquid: 1.1790g of reference U are taken₃O₈Adding 10ml of concentrated hydrochloric acid and 3ml of hydrogen peroxide into a beaker, then dripping 0.1ml of nitric acid into the beaker, placing the beaker on a heating electric furnace to heat the nitric acid by a small fire, stirring and dissolving the nitric acid to yellow transparent liquid, and then using distilled water to fix the volume to 1L to prepare uranium basic solution with the mass concentration of 1 g/L;

(3) water culture: preparing the base solution into substrate culture solutions with seven concentrations of 1 mg/L, 2 mg/L, 10 mg/L, 50 mg/L, 100 mg/L and 200 mg/L of uranium by using distilled water, setting 3-5 identical culture samples for each concentration, carrying out water culture, and subsequently supplementing improved Hoagland nutrient solution without uranium according to the consumption of the substrate culture solutions;

(4) and sampling and detecting: taking fresh plant leaves cultured at different concentrations every 5-7 days, cleaning, removing coarse veins, mashing, and performing parameter determination and recording, wherein the measurement parameters comprise chlorophyll a, chlorophyll b, carotenoid, soluble protein, Malondialdehyde (MDA) and uranium enrichment content, and superoxide dismutase (SOD), Catalase (CAT) and Peroxidase (POD) activities.

2. The method for measuring biochemical indications of plants under radioactive stress as claimed in claim 1, wherein the illumination time in the culture environment in said step of water culture is 24 hours a day.

3. The method for measuring biochemical indications of plants under radioactive stress as claimed in claim 1 or 2, wherein the temperature of the culture environment in the step of water culture is controlled at 25-30 ℃ and the humidity is controlled at 60-80%.

4. The method for measuring biochemical indications of plants under radioactive stress as claimed in claim 1 or 2, wherein the step of sampling and detecting comprises selecting plants in the range of 7 days to 35 days during water culture.

5. The method for measuring biochemical indications of plants under radioactive stress as claimed in claim 3, wherein said sampling and detecting step comprises selecting plants from the range of 7 th day to 35 th day during water culture.

6. The method for measuring the biochemical indications of plants under radioactive stress as claimed in claim 1 or 2, wherein the test plants selected in the material selection process are red full of river, hyacinth, dicranopteris pedata, humifuse euphorbia herb, dayflower and rohdea japonica.

7. The method for measuring the biochemical indications of plants under radioactive stress as claimed in claim 3, wherein the test plants selected in the material selection process are Azurita indica, hyacinth, Osmunda japonica, Euphorbia humifusa, Commelina communis and Rohdea japonica.

8. The method for measuring the biochemical indications of plants under radioactive stress as claimed in claim 5, wherein the test plants selected in the material selection process are Azurita indica, hyacinth, Osmunda japonica, Euphorbia humifusa, Commelina communis and Rohdea japonica.