CN110833551A - Use of pyrazolopyrimidine derivatives for the treatment of acute pancreatitis - Google Patents
Use of pyrazolopyrimidine derivatives for the treatment of acute pancreatitis Download PDFInfo
- Publication number
- CN110833551A CN110833551A CN201810927819.0A CN201810927819A CN110833551A CN 110833551 A CN110833551 A CN 110833551A CN 201810927819 A CN201810927819 A CN 201810927819A CN 110833551 A CN110833551 A CN 110833551A
- Authority
- CN
- China
- Prior art keywords
- acute pancreatitis
- use according
- pancreatic
- manifested
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 206010033645 Pancreatitis Diseases 0.000 title claims abstract description 50
- 206010033647 Pancreatitis acute Diseases 0.000 title claims abstract description 50
- 201000003229 acute pancreatitis Diseases 0.000 title claims abstract description 50
- OGEBRHQLRGFBNV-RZDIXWSQSA-N chembl2036808 Chemical class C12=NC(NCCCC)=NC=C2C(C=2C=CC(F)=CC=2)=NN1C[C@H]1CC[C@H](N)CC1 OGEBRHQLRGFBNV-RZDIXWSQSA-N 0.000 title claims abstract description 23
- 210000004923 pancreatic tissue Anatomy 0.000 claims abstract description 30
- 239000003814 drug Substances 0.000 claims abstract description 29
- 206010030113 Oedema Diseases 0.000 claims abstract description 13
- 230000017074 necrotic cell death Effects 0.000 claims abstract description 13
- 230000008595 infiltration Effects 0.000 claims abstract description 9
- 238000001764 infiltration Methods 0.000 claims abstract description 9
- 210000004969 inflammatory cell Anatomy 0.000 claims abstract description 8
- 102000002045 Endothelin Human genes 0.000 claims description 14
- 108050009340 Endothelin Proteins 0.000 claims description 14
- ZUBDGKVDJUIMQQ-UBFCDGJISA-N endothelin-1 Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(O)=O)NC(=O)[C@H]1NC(=O)[C@H](CC=2C=CC=CC=2)NC(=O)[C@@H](CC=2C=CC(O)=CC=2)NC(=O)[C@H](C(C)C)NC(=O)[C@H]2CSSC[C@@H](C(N[C@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(=O)N2)=O)NC(=O)[C@@H](CO)NC(=O)[C@H](N)CSSC1)C1=CNC=N1 ZUBDGKVDJUIMQQ-UBFCDGJISA-N 0.000 claims description 14
- 238000002360 preparation method Methods 0.000 claims description 9
- 239000008194 pharmaceutical composition Substances 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 6
- 238000009472 formulation Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000003405 delayed action preparation Substances 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 3
- 238000002347 injection Methods 0.000 claims description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 2
- AFVFQIVMOAPDHO-UHFFFAOYSA-N Methanesulfonic acid Chemical compound CS(O)(=O)=O AFVFQIVMOAPDHO-UHFFFAOYSA-N 0.000 claims description 2
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 2
- 239000004480 active ingredient Substances 0.000 claims description 2
- 239000002775 capsule Substances 0.000 claims description 2
- 238000013270 controlled release Methods 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 239000012729 immediate-release (IR) formulation Substances 0.000 claims description 2
- 239000000546 pharmaceutical excipient Substances 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 238000013268 sustained release Methods 0.000 claims description 2
- 239000012730 sustained-release form Substances 0.000 claims description 2
- 239000003826 tablet Substances 0.000 claims description 2
- RZWIIPASKMUIAC-VQTJNVASSA-N thromboxane Chemical compound CCCCCCCC[C@H]1OCCC[C@@H]1CCCCCCC RZWIIPASKMUIAC-VQTJNVASSA-N 0.000 claims description 2
- 229940079593 drug Drugs 0.000 abstract description 23
- 210000004027 cell Anatomy 0.000 abstract description 17
- 230000000694 effects Effects 0.000 abstract description 9
- 101001033233 Homo sapiens Interleukin-10 Proteins 0.000 abstract description 6
- 102000052620 human IL10 Human genes 0.000 abstract description 6
- 208000024891 symptom Diseases 0.000 abstract description 3
- 230000001225 therapeutic effect Effects 0.000 abstract description 3
- 241000700159 Rattus Species 0.000 description 17
- 239000000243 solution Substances 0.000 description 10
- 238000012360 testing method Methods 0.000 description 7
- OIXLLKLZKCBCPS-RZVRUWJTSA-N (2s)-2-azanyl-5-[bis(azanyl)methylideneamino]pentanoic acid Chemical compound OC(=O)[C@@H](N)CCCNC(N)=N.OC(=O)[C@@H](N)CCCNC(N)=N OIXLLKLZKCBCPS-RZVRUWJTSA-N 0.000 description 5
- 210000000683 abdominal cavity Anatomy 0.000 description 5
- 208000032843 Hemorrhage Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 4
- 238000000465 moulding Methods 0.000 description 4
- 101000932478 Homo sapiens Receptor-type tyrosine-protein kinase FLT3 Proteins 0.000 description 3
- 102100020718 Receptor-type tyrosine-protein kinase FLT3 Human genes 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 239000003112 inhibitor Substances 0.000 description 3
- 230000003902 lesion Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 210000000440 neutrophil Anatomy 0.000 description 3
- 210000000496 pancreas Anatomy 0.000 description 3
- 230000001575 pathological effect Effects 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 102000003814 Interleukin-10 Human genes 0.000 description 2
- 108090000174 Interleukin-10 Proteins 0.000 description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-N L-arginine Chemical compound OC(=O)[C@@H](N)CCCN=C(N)N ODKSFYDXXFIFQN-BYPYZUCNSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 210000001772 blood platelet Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 230000000762 glandular Effects 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 229940076144 interleukin-10 Drugs 0.000 description 2
- 208000028867 ischemia Diseases 0.000 description 2
- 230000004089 microcirculation Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000036285 pathological change Effects 0.000 description 2
- 231100000915 pathological change Toxicity 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- DSNBHJFQCNUKMA-SCKDECHMSA-N thromboxane A2 Chemical compound OC(=O)CCC\C=C/C[C@@H]1[C@@H](/C=C/[C@@H](O)CCCCC)O[C@@H]2O[C@H]1C2 DSNBHJFQCNUKMA-SCKDECHMSA-N 0.000 description 2
- 208000031261 Acute myeloid leukaemia Diseases 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 208000033776 Myeloid Acute Leukemia Diseases 0.000 description 1
- 206010028851 Necrosis Diseases 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 201000004681 Psoriasis Diseases 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 206010047139 Vasoconstriction Diseases 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 206010060926 abdominal symptom Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002497 edematous effect Effects 0.000 description 1
- 210000003989 endothelium vascular Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 230000000004 hemodynamic effect Effects 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000002757 inflammatory effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 210000002464 muscle smooth vascular Anatomy 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 210000003200 peritoneal cavity Anatomy 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 208000010110 spontaneous platelet aggregation Diseases 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 208000037816 tissue injury Diseases 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 210000004509 vascular smooth muscle cell Anatomy 0.000 description 1
- 230000002227 vasoactive effect Effects 0.000 description 1
- 230000025033 vasoconstriction Effects 0.000 description 1
- 230000003639 vasoconstrictive effect Effects 0.000 description 1
- 239000005526 vasoconstrictor agent Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
- A61K31/519—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim ortho- or peri-condensed with heterocyclic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
Landscapes
- Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Epidemiology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to the technical field of medicines, in particular to application of pyrazolopyrimidine derivative in treatment of acute pancreatitis. The pyrazolopyrimidine derivative has a good treatment effect on acute pancreatitis, can obviously improve the edema degree, inflammatory cell infiltration, cell necrosis and other symptoms of pancreatic tissues, and obviously reduce ET and TXA in the pancreatic tissues2The therapeutic effect of the recombinant human interleukin-10 is equal to or even better than that of the recombinant human interleukin-10.
Description
Technical Field
The invention relates to the technical field of medicines, in particular to application of pyrazolopyrimidine derivative in treatment of acute pancreatitis.
Background
Acute Pancreatitis (AP) is one of the clinically common Acute abdominal symptoms, which is caused by excessive activation of pancreatin in pancreatic tissues due to various causes, thereby causing a series of inflammatory reactions such as self digestion, hemorrhage, edema, necrosis of the pancreatic tissues and the like, with or without function changes of multiple organs. The disease incidence rate is about 13-45/100000 in China, and if 20% of patients do not take timely and effective treatment measures, the disease condition can further progress to Severe Acute Pancreatitis (SAP), the SAP condition is complex, the disease course is dangerous, the treatment difficulty is high, and the death rate is as high as 30%.
The AP Atlanta diagnostic standard in 2012 further standardizes the clinical classification of the AP on the original basis, and experts and scholars consistently classify the AP into three categories, namely: three major categories, namely m ild acueperations (MAP), Moderate Severe Acute Pancreatitis (MSAP) and Severe Acute Pancreatitis (SAP), make the classification of AP more accurate, and have great guiding significance for clinical staging, treatment and prognosis improvement.
The pyrazolopyrimidine derivative (formula I) is a small molecule inhibitor targeted on FLT3 kinase, is a novel compound independently developed by the applicant, and the patent of the compound is granted in China, the United states, Japan and other areas at present.
Only the pyrazolopyrimidine derivative is reported to treat acute myelogenous leukemia and psoriasis at present, and the application of the pyrazolopyrimidine derivative in treating acute pancreatitis is not seen.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides the application of the pyrazolopyrimidine derivative in treating acute pancreatitis.
The purpose of the invention can be realized by the following technical scheme:
application of pyrazolopyrimidine derivative shown as formula I or pharmaceutically acceptable salt and hydrate thereof in preparation of medicine for treating acute pancreatitis
Preferably, the acute pancreatitis is manifested by an increase in endothelin levels in pancreatic tissue.
Preferably, the acute pancreatitis is manifested by thromboxane A in pancreatic tissue2The level increased.
Preferably, the acute pancreatitis is manifested by edema of pancreatic tissue.
Preferably, the acute pancreatitis is manifested by infiltration of inflammatory cells by pancreatic tissue.
Preferably, the acute pancreatitis is manifested by the phenomenon of cellular necrosis of pancreatic tissue.
Preferably, the acute pancreatitis comprises mild acute pancreatitis, moderate severe acute pancreatitis and severe acute pancreatitis.
Preferably, the pharmaceutically acceptable salts include hydrochloride, sulfate, mesylate, phosphate.
The invention also provides application of the pharmaceutical composition in preparing a medicine for treating acute pancreatitis, which is characterized in that the pharmaceutical composition comprises pyrazolopyrimidine derivative shown as formula I or pharmaceutically acceptable salt or hydrate thereof as an active ingredient and a pharmaceutically acceptable excipient.
Preferably, the pharmaceutical composition is an injection preparation, an oral preparation, an external preparation, a sustained release preparation, or a controlled release preparation.
Preferably, the oral preparation comprises tablets, granules and capsules.
Preferably, the pharmaceutical composition is a controlled release formulation, a sustained release formulation, an immediate release formulation.
Endothelin (ET) is one of the most potent vasoactive substances known at present, and its content in vascular smooth muscle cells is high, with a strong and long-lasting vasoconstrictive action after activation. The ET can participate in the whole process of generation and development of AP, the action mechanism of the ET can be that the ET acts on vascular smooth muscle, the permeability of pancreatic capillary vessels is increased, leakage is increased, blood flow is reduced, receptors are activated at the same time, calcium concentration overload occurs in cells through signal conduction, formed calcium salt can block microcirculation to a certain extent, pancreatic capillary blood vessels leak, blood concentration and viscosity increase in pancreas are caused to a certain extent, blood platelets aggregate to form tiny thrombus, the hemodynamics in pancreas is changed, microcirculation stasis is caused, pancreatic cell ischemia and hypoxia are caused, AP is induced or the original condition is aggravated, and AP is converted into SAP.
Thrombin A2(TXA2) Is an endogenous inflammatory factor, and the source in the body is mainly platelets (AP can be synthesized by pancreatic acinar cells). The body activates TXA after being stimulated by external or internal stimuli2The combination of its activity and corresponding substances on the vascular endothelium exerts a strong vasoconstriction effect, and also has a platelet aggregation promoting effect, which can lead to pancreatic tissue ischemia, edema and even necrosis, and the AP disease progresses to SAP.
The pyrazolopyrimidine derivative is originally an FLT3 inhibitor, and researches show that the pyrazolopyrimidine derivative has a good treatment effect on acute pancreatitis, can obviously improve the edema degree, inflammatory cell infiltration, cell necrosis and other symptoms of pancreatic tissues, and obviously reduces ET and TXA in the pancreatic tissues2The therapeutic effect of the recombinant human interleukin-10 is equal to or even better than that of the recombinant human interleukin-10.
Compared with the prior art, the method has the following advantages:
the pyrazolopyrimidine derivative provided by the invention is a novel drug for treating acute pancreatitis, not only expands the selectivity of the existing drug for treating acute pancreatitis, provides more drug choices for effective treatment of acute pancreatitis, but also further expands the application range of the pyrazolopyrimidine derivative as an FLT3 inhibitor.
Drawings
FIG. 1 is a graph of HE staining of rat pancreatic tissue at 12h in the blank control group.
FIG. 2 is a graph showing HE staining of rat pancreatic tissue at 12h in the model group.
FIG. 3 is a graph of HE staining of rat pancreatic tissue at 12h in the positive drug group.
FIG. 4 is a graph of HE staining of rat pancreatic tissue at 12h in the pyrazolopyrimidine derivative group.
Fig. 5 is the acute pancreatitis tissue injury scoring criteria.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Example 1 Effect on rats with severe acute pancreatitis
1. Experimental animals: clean SD rats, male, with a body mass of 200-250 g, purchased from Beijing vitamin Tonglihua laboratory animal technology GmbH.
2. Experimental materials: pyrazolopyrimidine derivative was synthesized by the inventors. Pyrazolopyrimidine derivative was synthesized by the inventors. Mixing castor oil with ethanol 1: 1, passing through a sterile filter membrane of 0.22 mu m to obtain an ELE solution, weighing a certain mass of pyrazolopyrimidine derivative powder, dissolving the powder by using the ELE solution, adding a certain volume of sterilized water after the powder is completely dissolved, and uniformly mixing for later use. The volume ratio of the ELE solution to the sterile water is 1: 3. l-arginine (l-arginine, sigma), interleukin 10(rhIL-10, Protech).
3. Animal grouping: rats were randomly divided into a blank control group, a model group, a positive drug group, and a pyrazolopyrimidine derivative drug group (hereinafter referred to as a test drug group), and 30 rats were administered to each group. Each large group was then randomly divided by time limit into three subgroups, 3h, 6h, 12h groups, 10 each.
4. Preparing an animal model: except for the blank control group, the rats (see leiqiu, methodology of pharmacological experiments on chinese medicine, shanghai scientific press, second edition) were fasted and freely drunk for 12h before molding, and a 6% L-arginine (L-arginine) solution was slowly injected intraperitoneally at a dose of 150mg/10g, with the injection frequency: 1 time per hour for a total of 3 times. The successful modeling inspection standard is that the pancreatic tissue is observed by naked eyes to show edema and hemorrhage, part of the tissue has necrosis, the calcification spots scattered in the pancreatic tissue can be seen, the abdominal cavity is dilated, a large amount of bloody ascites can be seen after the abdominal opening, the capillary vessels of the intestinal wall are obviously dilated, the pancreatic lobular edema can be seen under an optical microscope, a large amount of inflammatory cells are infiltrated, the range of the affected pancreatic cells is wide, and a plurality of necrotic pancreatic cells can be seen under the visual field.
5. The administration scheme is as follows: (1) normal control group, model group: and 3 days before molding, 10mL/kg of mixed solution of the ELE solution and distilled water in a volume ratio of 1:3 is administered by intragastric administration for 3 consecutive days. Model groups then inject L-arginine (L-arginine) solution into the peritoneal cavity as per "step 4".
(2) A positive drug group: 1h before molding, the diluted human recombinant interleukin 10 is injected into the abdominal cavity according to the dose of 10000 IU/mouse. Then, according to "step 4", L-arginine (L-arginine) solution is injected into the abdominal cavity.
(3) Test drug groups: 3 days before molding, the rats were gavaged with 12mg/kg/d (10mL/kg, 1.2 mg/mL) for 3 consecutive days. Then, according to "step 4", L-arginine (L-arginine) solution is injected into the abdominal cavity.
6. Collecting a specimen: the timing was started by the last intraperitoneal injection of 6% L-arginine (L-arginine) solution into the rat cavity. At three time points of 3h, 6h and 12h, the rats are sacrificed in batches, the abdominal cavity is fully opened, the visual field is exposed, the position of the pancreas is found, the pancreas tissues are quickly separated and cut under the aseptic condition, and part of the tissues are put into paraformaldehyde solution with the volume fraction of 4 percent for preparing pathological sections and observing under an optical microscope. The remaining part was put into an EP tube previously supplied with 1.5ml of physiological saline, sufficiently crushed and ground, and then centrifuged at 3000 rpm for 10 minutes in a low-temperature centrifuge, and the supernatant was taken. Subpackaging the supernatant, and freezing at-20 deg.C for detecting ET, NO and TXA2、PGI2And (4) content.
7. Detecting the index
(1) And (3) detecting the pathological histology: at the end of the experiment, rat pancreatic tissue was removed, routinely HE stained, 10 fields of view were observed per section, and histological scoring was performed with reference to the severity of tissue edema, inflammation, bleeding and cell necrosis according to Rongione et al (see figure 5 of the specification).
(2) Determination of ET and TXA in rat pancreatic tissue by enzyme-linked immunosorbent assay (ELISA)2The content of (a). The operation is referred to the reagent instructions.
8. The statistical method comprises the following steps: data were processed using SPSS17.0, and the measurement data were expressed as (mean ± standard deviation), with uniform variance analyzed using variance, and non-uniform variance analyzed using rank sum test, with P <0.05 being statistically significant.
9. Results of the experiment
(1) Histopathological examination results
As shown in figure 1, the sections of the blank control group can be seen to have clear pancreatic lobule structures, complete acinar structures and no obvious pathological changes, and only individual sections can be seen to have small amount of inflammatory cell infiltration, mild edema among lobules and limited range.
As shown in FIG. 2, the lesions of the model group are obvious, and it can be seen that the pancreatic lobular space is widened, the edema of the pancreatic lobules and the lobular space is obvious, a large amount of neutrophil infiltration is carried out around the glandular duct and in the acinar cells, the focal vacuole degeneration of the acinar cells in the lobules, and the punctate necrosis of part of the acinar cells appears. The most obvious change is the section of the 12h node, and the neutrophils are distributed and infiltrated in a large area under the visual field; many scattered bleeding and cell necrosis, incomplete structure of lobule, and different degrees.
As shown in fig. 3, under the light microscope of the positive drug group, it can be seen that the pancreatic leaflet gap is widened, the pancreatic leaflets and the leaflet gap are edematous, the neutrophil infiltration around the glandular duct and in the acinar cells is wide, the focal vacuolar degeneration of the acinar cells can be seen in the leaflets, and some acinar cells can be seen in punctate necrosis, but the pathological change is lighter than that of the model group in degree.
As shown in FIG. 4, the test drug group pancreatic section showed similar changes to the model group and the positive drug group, but the lesion extent was relatively reduced in the lesion coverage, the degree of edema of the pancreatic lobules, the infiltration of inflammatory cells, and the number of necrosis of cells, compared with the above two groups.
(2) Comparison of pancreatic histopathological scoring results at different time points
TABLE 1 comparison of pancreatic histopathology score results at different time points
P <0.05 compared to the blank control group and △ P <0.05 compared to the model group.
As can be seen from Table 1, compared with the model group, the tested drug group can significantly reduce pathological scores (P <0.05) of rat pancreatic tissues for 3h, 6h and 12h, and the effect is equivalent to or even superior to that of the positive drug group.
(3) Pancreatic tissue ET content level changes
TABLE 2 pancreatic tissue ET content variation at different time points
| Group of | 3h | 6h | 12h |
| Blank control group | 35.08±0.82 | 34.15±0.64 | 32.12±1.63 |
| Model set | 88.26±1.72* | 148.02±2.08* | 165.31±3.52* |
| Positive drug group | 80.15±1.24△ | 124.77±2.52△ | 142.25±2.12△ |
| Test drug group | 86.37±2.20 | 132.85±1.82△ | 140.73±2.36△ |
P <0.05 compared to the blank control group and △ P <0.05 compared to the model group.
As shown in Table 2, compared with the model group, the tested drug group can remarkably reduce the ET content in the pancreatic tissues of rats at 6h and 12h, (P is less than 0.05), and the effect is equal to or even better than that of the positive drug group. The test showed only a decreasing trend at 3h, and was not statistically significant.
(4) Pancreatic tissue TXA2Change in content level
TABLE 3 pancreatic tissue TXA at different time points2Content change
| Group of | 3h | 6h | 12h |
| Blank control group | 280.46±18.35 | 282.95±20.14 | 281.74±15.63 |
| Model set | 475.28±61.32* | 688.30±65.41* | 912.23±72.63* |
| Positive drug group | 314.76±29.36△ | 463.71±35.94△ | 662.45±58.31△ |
| Test drug group | 322.58±25.72△ | 428.59±42.15△ | 548.26±48.92△ |
P <0.05 compared to the blank control group and △ P <0.05 compared to the model group.
As shown in Table 3, the tested drug groups significantly reduced TXA at 6h and 12h in rat pancreatic tissue compared with the model group2(P) content of<0.05), the effect is equal to or even better than that of the positive medicine group.
Conclusion
The pyrazolopyrimidine derivative has a good treatment effect on acute pancreatitis, can obviously improve the edema degree, inflammatory cell infiltration, cell necrosis and other symptoms of pancreatic tissues, and obviously reduce ET and TXA in the pancreatic tissues2The therapeutic effect of the recombinant human interleukin-10 is equal to or even better than that of the recombinant human interleukin-10.
Although the invention has been described in detail hereinabove by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that many modifications and improvements can be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (12)
1. Application of pyrazolopyrimidine derivative shown as formula I or pharmaceutically acceptable salt and hydrate thereof in preparation of medicine for treating acute pancreatitis
2. The use according to claim 1, characterized in that said acute pancreatitis is manifested by an increase in the level of endothelin in the pancreatic tissues.
3. Use according to claim 1, characterized in that said acute pancreatitis is manifested by thromboxane A in the pancreatic tissue2The level increased.
4. The use according to claim 1, wherein the acute pancreatitis is manifested by edema in the pancreatic tissue.
5. The use according to claim 1, characterized in that said acute pancreatitis is manifested by infiltration of inflammatory cells in the pancreatic tissues.
6. Use according to claim 1, characterized in that said acute pancreatitis is manifested by the phenomenon of cellular necrosis of the pancreatic tissue.
7. The use according to claim 1, wherein the acute pancreatitis includes mild acute pancreatitis, moderate severe acute pancreatitis, severe acute pancreatitis.
8. Use according to claim 1, wherein the pharmaceutically acceptable salts comprise hydrochloride, sulfate, mesylate, phosphate.
9. Use of a pharmaceutical composition for preparing a medicament for treating acute pancreatitis, wherein the pharmaceutical composition comprises the pyrazolopyrimidine derivative represented by the formula i in claim 1, or a pharmaceutically acceptable salt or hydrate thereof, as an active ingredient, and a pharmaceutically acceptable excipient.
10. The use according to claim 9, wherein the pharmaceutical composition is an injection preparation, an oral preparation, an external preparation, a sustained-release preparation, or a controlled-release preparation.
11. The use according to claim 10, wherein the oral formulation comprises tablets, granules, capsules.
12. The use according to claim 9, wherein the pharmaceutical composition is a controlled release formulation, a sustained release formulation, an immediate release formulation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810927819.0A CN110833551B (en) | 2018-08-15 | 2018-08-15 | Use of pyrazolopyrimidine derivatives for the treatment of acute pancreatitis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810927819.0A CN110833551B (en) | 2018-08-15 | 2018-08-15 | Use of pyrazolopyrimidine derivatives for the treatment of acute pancreatitis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110833551A true CN110833551A (en) | 2020-02-25 |
| CN110833551B CN110833551B (en) | 2023-03-24 |
Family
ID=69573073
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810927819.0A Active CN110833551B (en) | 2018-08-15 | 2018-08-15 | Use of pyrazolopyrimidine derivatives for the treatment of acute pancreatitis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110833551B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200406388A (en) * | 2002-08-14 | 2004-05-01 | Vertex Pharma | Protein kinase inhibitors and uses thereof |
| WO2005007073A2 (en) * | 2003-07-17 | 2005-01-27 | Gamida-Cell Ltd. | Ex vivo progenitor and stem cell expansion for use in the treatment of disease of endodermally-derived organs |
| CN1863780A (en) * | 2002-08-14 | 2006-11-15 | 沃泰克斯药物股份有限公司 | Protein kinase inhibitors and uses thereof |
| CN101675051A (en) * | 2007-03-21 | 2010-03-17 | 惠氏公司 | Pyrazolopyrimidine analogs and their use as mtor kinase and pi3 kinase inhibitors |
| CN102018689A (en) * | 2009-09-16 | 2011-04-20 | 北京大学 | New application of curcumin |
| WO2011106168A1 (en) * | 2010-02-24 | 2011-09-01 | Dcam Pharma Inc | Purine compounds for treating autoimmune and demyelinating diseases |
| CN105769851A (en) * | 2016-03-11 | 2016-07-20 | 张雪燕 | Medicinal composition for treating severe acute pancreatitis and application thereof |
| CN107001283A (en) * | 2014-07-31 | 2017-08-01 | 国家健康与医学研究院 | Flt3 receptor antagonists |
-
2018
- 2018-08-15 CN CN201810927819.0A patent/CN110833551B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW200406388A (en) * | 2002-08-14 | 2004-05-01 | Vertex Pharma | Protein kinase inhibitors and uses thereof |
| CN1863780A (en) * | 2002-08-14 | 2006-11-15 | 沃泰克斯药物股份有限公司 | Protein kinase inhibitors and uses thereof |
| WO2005007073A2 (en) * | 2003-07-17 | 2005-01-27 | Gamida-Cell Ltd. | Ex vivo progenitor and stem cell expansion for use in the treatment of disease of endodermally-derived organs |
| CN101675051A (en) * | 2007-03-21 | 2010-03-17 | 惠氏公司 | Pyrazolopyrimidine analogs and their use as mtor kinase and pi3 kinase inhibitors |
| CN102018689A (en) * | 2009-09-16 | 2011-04-20 | 北京大学 | New application of curcumin |
| WO2011106168A1 (en) * | 2010-02-24 | 2011-09-01 | Dcam Pharma Inc | Purine compounds for treating autoimmune and demyelinating diseases |
| CN107001283A (en) * | 2014-07-31 | 2017-08-01 | 国家健康与医学研究院 | Flt3 receptor antagonists |
| CN105769851A (en) * | 2016-03-11 | 2016-07-20 | 张雪燕 | Medicinal composition for treating severe acute pancreatitis and application thereof |
Non-Patent Citations (6)
| Title |
|---|
| JING D,ET AL: "Expression of Reg family proteins in embryonic stem cells and its modulation by Wnt/beta-catenin signaling", 《STEM CELLS DEV》 * |
| MA S，ET AL: "SKLB-677, an FLT3 and Wnt/β-catenin signaling inhibitor, displays potent activity in models of FLT3-driven AML", 《SCIENTIFIC REPORTS》 * |
| SURYAWANSHI A，ET AL: "Modulation of Inflammatory Responses by Wnt/β-Catenin Signaling in Dendritic Cells: A Novel Immunotherapy Target for Autoimmunity and Cancer", 《FRONT IMMUNOL》 * |
| XIAO W，ET AL: "Retinoic Acid Ameliorates Pancreatic Fibrosis and Inhibits the Activation of Pancreatic Stellate Cells in Mice with Experimental Chronic Pancreatitis via Suppressing the Wnt/β-Catenin Signaling Pathway", 《PLOS ONE》 * |
| 朱芳丽等: "ICAM-1和SF检测在急性胰腺炎严重程度评价中的价值", 《中国中西医结合消化杂志》 * |
| 陈晓理，等: "常用免疫抑制剂对急性胰腺炎免疫异常的调节作用", 《中华肝胆外科杂志》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110833551B (en) | 2023-03-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI421083B (en) | Methods and compositions for inhibiting angiogenesis | |
| CN108289881A (en) | Sai Nikeweiluo conjoint therapies for treating fibrosis | |
| JP5999724B2 (en) | Pharmaceutical composition comprising an HDAC inhibitor and a steroid and use thereof | |
| CN108619490A (en) | A kind of new application of the people source fibroblast growth factor of long-actingization mutation | |
| Bastia et al. | NCX 667, a novel nitric oxide donor, lowers intraocular pressure in rabbits, dogs, and non-human primates and enhances TGFβ2-induced outflow in HTM/HSC constructs | |
| JP6626094B2 (en) | Use of ginsenoside M1 for inhibiting renal fibrosis | |
| CN110743016A (en) | Combined pharmaceutical composition for resisting colorectal cancer and application thereof | |
| CN109771411A (en) | Use of dihydroquercetin in preparing medicine for treating fatty liver | |
| CN110870858A (en) | Pharmaceutical compositions comprising organic acid acidifying agents and conventional ineffective compounds and uses thereof | |
| CN113244233A (en) | Application of piperlongumine in preparation of medicine for preventing and treating corneal transplantation immunological rejection | |
| CN110833551B (en) | Use of pyrazolopyrimidine derivatives for the treatment of acute pancreatitis | |
| CN111803488B (en) | Application of atractylenolide II in preparation of anti-renal fibrosis medicine and anti-renal fibrosis medicine | |
| CN103251585A (en) | Effects and application of artemisinin and its derivative in inhibition of platelet-derived growth factor receptor A | |
| CN111358780B (en) | Application of formononetin in preparation of medicine for preventing and treating severe acute pancreatitis, tablet, dripping pill and injection emulsion | |
| WO2017157248A1 (en) | Use of triacetyl-3-hydroxyphenyladenosine in preparing pharmaceuticals for treatment of atherosclerosis | |
| CN101550135A (en) | The preparation method of AS-605240 and its application in the preparation of medicines for treating inflammatory diseases | |
| CN101411705A (en) | Pharmaceutical composition for preventing and treating atherosclerosis | |
| Ji et al. | Stigmast-4-en-3-one from Euonymus alatus (Thunb.) Siebold. improves diabetic retinopathy and angiogenesis mediated by glucocorticoids receptor | |
| CN112438942A (en) | Pharmaceutical composition containing alkalizer and its synergist and its application | |
| WO2022221988A1 (en) | Pharmaceutical hydronidone formulations for diseases | |
| CN110870860A (en) | Pharmaceutical composition comprising amino acid nutrients and conventional ineffective compounds and use thereof | |
| CN115737661A (en) | Application of baicalin in preparation of medicine for inhibiting retinal neovascularization | |
| CN108969517B (en) | Application of ginkgolide composition in preparation of medicine for treating renal tissue fibrosis | |
| CN110833550B (en) | Application of pyrazolopyrimidine derivative in treatment of liver injury caused by acute pancreatitis | |
| CN112707806A (en) | EphB2 small-molecule inhibitor and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |